CN103299963A - Manual expanding propagation method and special equipment thereof for worm grass host insects - Google Patents

Manual expanding propagation method and special equipment thereof for worm grass host insects Download PDF

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CN103299963A
CN103299963A CN2013102936149A CN201310293614A CN103299963A CN 103299963 A CN103299963 A CN 103299963A CN 2013102936149 A CN2013102936149 A CN 2013102936149A CN 201310293614 A CN201310293614 A CN 201310293614A CN 103299963 A CN103299963 A CN 103299963A
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caterpillar fungus
chinese caterpillar
stage
larva
fungus host
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CN103299963B (en
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刘飞
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Chongqing Academy of Chinese Materia Medica
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Chongqing Academy of Chinese Materia Medica
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Priority to PCT/CN2014/080065 priority patent/WO2015003545A1/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

Abstract

The invention provides a manual expanding propagation method and special equipment thereof for worm grass host insects. The manual expanding propagation method includes an egg stage, a larva stage, a pupa stage and an imago stage. The manual expanding propagation method is the method adopting single insect separating cultivating method in the larva stage and the pupa stage of the worm grass host insects. The condition that the host insects kill each other and spread diseases to each other is avoided; besides, a water leaking groove is arranged at the inner bottom of a box I, and water remaining in the water leaking groove is utilized to keep humidity of moisture-preserved materials. Structure of worm grass host insect cultivating plates is stable and can be overlapped during the process of host insect cultivation, so that space is effectively utilized and the manual expanding propagation method and the special equipment thereof for worm grass host insects can be utilized to produce in large scale.

Description

Artificial expanding propagation method and the special equipment thereof of Chinese caterpillar fungus host insect
Technical field
The present invention relates to a kind of insect raising method, relate to the artificial expanding propagation method of a kind of Chinese caterpillar fungus host insect and special equipment thereof in particular.
Technical background
Cordyceps has 507 kinds in the world, and wherein, the certified products Cordyceps sinensis of generally acknowledging is that section ergot fungus cordyceps sinensis Cordyceps SiI1cnsis (Berk.) sacc. colonizes in stroma on the Hepialidae insect larvae and the complex of larva corpse.
Also having some other Chinese caterpillar fungus in addition is respectively that cordyceps hawkesii gary has another name called the Huo Kesi Chinese caterpillar fungus.This product is that Clavicipitaceae fungi cordyceps hawkesii gary C.hawkesii Gray parasitizes stroma and the polypide on the lepidopterous insects larva.Liangshan cordyceps herb is for Clavicipitaceae fungi liangshan cordyceps herb Cliangshanensis Zang I Jut Hu parasitizes stroma and polypide on the lepidopterous insects larva.
The branch Chinese caterpillar fungus is for Clavicipitaceae fungi branch Chinese caterpillar fungus C.rarnosa Teng parasitizes stroma and polypide on the lepidopterous insects larva.The pupa grass has another name called northern Chinese caterpillar fungus, is Clavicipitaceae fungi pupa grass C.militaris(L.)I?ink。Parasitize stroma and polypide on the Noctuidae larva.Incense stick cordycep is for Clavicipitaceae fungi incense stick cordycep C.barnesis Thwaites parasitizes stroma and polypide on the lepidopterous insects larva.
Chinese patent application (publication number: CN19700734A) disclose a kind of production method of Cordyceps sinensis; Chinese patent application (publication number: CN1970733A) disclose a kind of method of cultivating Cordyceps sinensis at hepialus genus captive field; Chinese patent application (publication number: CN1948456A) disclose a kind of method that improves yield of Qinghai Tibet plateau cordyceps; Chinese patent application (publication number: CN19484757A) disclose a kind of method that improves yield of Qinghai Tibet plateau cordyceps; as if the disclosed just content of described four kinds of methods is to be transformed environment and sets up warm livestock shed and support the bat moth larvae under Qinghai-Tibet wild environment; increase the quantity of stable breeding zone adult or larva; to improve the survival rate of bat moth larvae; grow to 6-8 during age at the bat moth larvae; by use the mode of microbiological contamination spore liquid to whole stable breeding place; the bat moth larvae is infected, and the bat moth larvae infects fully and removes warm and humid protection setting to allow it return to nature warm and humid.The process of growth of whole Cordyceps sinensis is all carried out in same region.
Summary of the invention
For solving the bigger problem of host's larvae cultivation difficulty in the above-mentioned Chinese caterpillar fungus plantation, the invention provides the artificial expanding propagation method of a kind of Chinese caterpillar fungus host insect, comprise ovum stage, larval stage, pupa stage and adult stage; Described method is larval stage and the pupa stage of Chinese caterpillar fungus host insect to adopt wall scroll to isolate breeding method.
Described wall scroll is isolated the specification requirement of cultivating Chinese caterpillar fungus:
1) cultivating air purity requires to be not less than 100,000 grades;
2) larva is placed on mass water content on the material which can retain moisture of 50-500%, wherein flowing water flow can not occur on the material which can retain moisture.
Described Chinese caterpillar fungus host insect is Lepidoptera Hepialidae insect.
The temperature of described cultivation is 8-25 ℃.
The Chinese caterpillar fungus host insect is artificial to be expanded numerous special equipment and by a bottom box body, partition apparatus (2), material which can retain moisture of infiltration groove is set and forms with lid four parts that partition apparatus is complementary; Described material which can retain moisture is arranged on the bottom of box body, and partition apparatus arranges on the material which can retain moisture, and lid covers partition apparatus top.
The numerous special equipment of the artificial expansion of described Chinese caterpillar fungus host insect has the material of black or other dark lucifuges to make.
Useful technique effect of the present invention is: 1) avoided cutting one another's throats and the mutual situation that infects disease of host larva; 2) other inner bottom surface at box body 1 is provided with the infiltration groove, not only can avoid occurring on the material which can retain moisture ponding, also utilizes the residual water of infiltration groove to keep the humidity of material which can retain moisture simultaneously; 3) structure of Chinese caterpillar fungus host larvae cultivation dish is firm, can carry out overlapping stacking in the process of cultivating the host larva, has effectively utilized the space, is fit to large-scale production.
Description of drawings
The combination schematic diagram of Fig. 1 Chinese caterpillar fungus host larvae cultivation dish;
The structural representation of Fig. 2 box body 1;
The schematic diagram of Fig. 3 partition apparatus 2;
The FB(flow block) of the artificial expanding propagation method of Fig. 4 Chinese caterpillar fungus host insect;
The structural representation of Fig. 5 emergence dish.
Wherein, 1 box body, the 1-1 groove that seeps water, 2 partition apparatus, 3 lids, 4 barrel-like structures, 4-1 gauze, 4-2 fixed disk.
Embodiment
Structure and the using method of embodiment 1 Chinese caterpillar fungus host larvae cultivation dish and emergence dish
Artificial numerous special equipment, the called after Chinese caterpillar fungus host larvae cultivation dish in concrete production of expanding of Chinese caterpillar fungus host insect.Described Chinese caterpillar fungus host larvae cultivation dish is made up of box body 1, material which can retain moisture, partition apparatus 2 and 3 four parts of lid; Wherein, described box body 1 is the box-like or disk-like structure of a upper opening, and at the bottom level of box body 1, and inner bottom surface is provided with the infiltration groove 1-1 of strip groove, infiltration groove 1-1 can be that parallel strip groove is formed, and also can be that many grooves intersections are formed.
Be provided with one deck at inner bottom surface and filter material which can retain moisture, this material can be sponge, glass fiber material; Also can be cotton, the absorbent material made of suction foam, be that water absorbing capacity is between mass water content 50%-500% for the requirement of above-mentioned material which can retain moisture; Material which can retain moisture is covered on the whole inner bottom surface of box body 1 in use.
Also be provided with partition apparatus 2 at box body 1 inner bottom surface that covers material which can retain moisture, described partition apparatus 2 is that the barrel-like structure by one or more both ends opens rearranges, as shown in Figure 1, after being placed on partition apparatus 2 on the material which can retain moisture, and after adding a cover lid 3, the barrel-like structure in each partition apparatus 2 forms an obturator.
When using Chinese caterpillar fungus host larvae cultivation dish, earlier material which can retain moisture is covered on the whole inner bottom surface of box body 1, lay partition apparatus 2 then, water spray makes the mass water content of material which can retain moisture reach 50-500% on the material which can retain moisture, then Chinese caterpillar fungus host larva is rendered to respectively in the box body of the upper opening that the inner bottom surface of partition apparatus 2 and box body 1 forms, throw in one in each box body, and drop into the feed that Chinese caterpillar fungus host larva need eat, also can add some simultaneously for the matrix (humus soil or artificial substratum) that larva is hidden, add a cover lid 3 then; Each Chinese caterpillar fungus host larva is cultivated in the space of an independent sealing, when the row row checks at set intervals lid 3 is opened, when needs are changed the cultivation dish, taking out partition apparatus 2 puts Chinese caterpillar fungus host larva in the another one Chinese caterpillar fungus host larvae cultivation dish in a suitable place to breed, the method of putting in a suitable place to breed is identical, then used Chinese caterpillar fungus host larvae cultivation dish cleaning and sterilizing is reused.
It is best that whole Chinese caterpillar fungus host larvae cultivation dish adopts the material of black or other dark lucifuges to make effect.
The emergence dish is made of greater than the barrel-like structure 4 of lower ending opening side by side more than one upper end open; The lower end of barrel-like structure 4 places on the fixed disk 4-2, and upper end open is provided be used to the gauze 4-1 that prevents that the host insect adult from escaping.Place material which can retain moisture at fixed disk 4-2 in use, again barrel-like structure 4 is placed on the wet stock, pupa is placed in the barrel-like structure 4, cover then and go up gauze 4-1; Pupa is placed within the space of a sealing.
The artificial expanding propagation method of embodiment 2 Chinese caterpillar fungus host insects is investigated
1, the collection of worm's ovum
Being collected in of worm's ovum need not cleanliness factor, and to require cleanliness factor be to carry out under the environmental condition between 300,000 grades.Gather the ovum that Hunan rod bat (Napialus hunanensis Chu et Wang) adult and Gongga bat moth adult produce respectively.Concrete collecting method is, in the region of gathering, the adult that sprouted wings in each cultivates grid the same day (or the adult of collecting in kind of the property rejuvenation zone) is placed into a supported mosquito net mating bed in (by gauze or can be ventilated but can avoid the material of adult escape to make), the inner suspension of mosquito net or the thing that some adults of placement can adhere in containing the ovum frame, such as greenweed, the branch that green leaf is arranged or bamboo bifurcation etc., the end that mosquito net hangs places to be contained in the ovum frame.The mosquito net mating bed that is placed with adult is placed in the environment of natural daylight cycle and natural daylight intensity, can play natural environment weather sound and the image (main purpose is its natural environmental condition of imitation) of corresponding time period with large projector in the environment, and under 8-25 ℃ condition, leave female adult and male imago mating, adult can mating in the life cycle of 5-15d 1-3 time.Whole process is to carry out under the condition of 60-70% in humidity.Post-coitum just begins to lay eggs soon.The temperature of laying eggs control is controlled at 60-70% at 8-25 ℃, humidity; Under the condition of natural daylight cycle and natural daylight intensity, carry out.The ovum that adult gives birth in mosquito net is scattering into the bottom of mosquito net mating bed---and contain in the ovum frame, contain dry soft filter paper of ovum frame bottom placement or cotton or other fibrous material and be used for collecting the ovum of giving birth to.During collection with mosquito net from it and contain ovum frame junction and separate the back to collect mosquito net terminal and raise, move Sheng ovum frame then out, simultaneously the mosquito net end is placed in the Sheng ovum frame of new preparation and collects the ovum of newly giving birth to.Take out and contain the ovum frame collecting the dry filter paper of ovum or cotton or other fibrous material, remove impurity, and ovum is focused in the steel wire that can not spill.The time of ovum collecting is every day 3 times, is respectively morning, noon and evening.Being collected under 8-25 ℃ the condition of ovum finished.Whole process is to carry out under the condition of 60-70%, natural daylight cycle and natural daylight intensity in humidity.
After ovum collecting is intact, transfers under the environmental condition that the cleanliness factor requirement is not less than 100,000 grades and carry out the cleaning of ovum and the hatching of ovum; The staff requires to dress work clothes, mouth mask and cap.Submerge and wash about 10 seconds to flowing water in the cold water of the 60-100 ℃ of sterilization of heating just focusing on ovum in the steel wire, adopt same procedure to clean at interval after 1-2 minute the 2nd time, adopt same procedure to clean at interval after 1-2 minute the 3rd time, hatch stage by stage after having cleaned.Phase I is to have cleaned when just having begun to hatch to ovum to end, ovum places on filter paper with certain humidity or cotton or other fibrous material hatches, what standard of humidity is water breakthrough and lose the humidity degree of flowing water when tilting, with preserving moisture through the cold water of the 60-100 ℃ of sterilization of heating, incubation temperature is 8-25 ℃, and illumination condition is natural daylight intensity and photoperiod; Second stage be ovum begin hatching up to all ovum all hatching finish and end, all ovum are transferred under the anhydrous condition are hatched.Use the cold water flowing water through the 60-100 ℃ of sterilization of heating to wash about 10 seconds every 2-3 days during the phase I of ovum hatching, and change preserve moisture filter paper or cotton or other fibrous material of hatching, thereby reach the cleaning of ovum.Collect the larva that hatches respectively, obtain the larva of Hunan rod bat and the larva of Gongga bat moth.
2, the raising of Chinese caterpillar fungus host larva
The raising of Chinese caterpillar fungus host larva requires to be not less than at cleanliness factor under 100,000 grades the environmental condition to be carried out; The staff requires to dress work clothes, mouth mask and cap.1000 of the larvas of the Gongga bat moth that the collection said method obtains are given Gongga by name bat moth group; 1000 of the larvas of the Hunan rod bat that the collection said method obtains organize for Hunan by name rod bat moth.
1) processing of Gongga bat moth group comprises intensive farm stage and wall scroll isolated rearing stage;
Being treated to of intensive farm stage wherein: 1000 of the larvas that has just hatched under drying condition are divided into 10 rent, transfer to respectively in the plateau humus soil (also available artificial matrix replaces) of a small amount of rhizome that mainly is mixed with serpentgrass, chrysanthemum ginger, carrot and life fruit and other plateau plants and raise.Humus soil is firmly held with hand can be agglomerating, and hand is decontroled the humidity standard that can not loose.The raising temperature condition is 8-18 ℃.Stocking density is that unit raises the box (amount of no more than 30 larvas of input in the long 30cm * wide 20cm height * 15cm).Since the mobility of newly hatched larvae a little less than, scope of activities is little, a little less than the injury between the individuality, so newly hatched larvae is fit to intensive farm.The about 30-60 of newly hatched larvae intensive farm days time, (a situation arises determines the concrete time according to raising temperature and disease.In 8-18 ℃ raising temperature scope, raising temperature is more low, and the time of confinement rearing is longer relatively; Disease takes place more serious, and the time of confinement rearing is shorter relatively).During this time can be according to the rotten degree of feed and how many replacings or interpolation feed and the matrix of disease.When replacing or interpolation larvae feeds or matrix, the staff must wear the fingerstall of avoiding disease to infect except conventional antiforeign bacteria pollutes dressing, touched the instrument of disease polypide or pathogen in the operating process and will in time change.In the feed process, serpentgrass, chrysanthemum ginger and carrot are cleaned with ultrasonic washing instrument after cleaning with clear water again, and it is edible for newly hatched larvae to be processed into fritter afterwards in dust proof workshop; The life fruit is cleaned the back with clear water and cleans with ultrasonic, and is directly edible for larva then.Serpentgrass, chrysanthemum ginger, carrot and life fruit all will be with that rot or too small cleaning out after first being processed or processing.
Being treated to of wall scroll isolated rearing stage: the larva of raising the about 30-60d of confinement rearing in the box all taken out and larva is placed wall scroll raising in the Chinese caterpillar fungus host larvae cultivation dish that embodiment 1 provides.Every larva lives in independently in the space, can add the matrix (humus soil or artificial substratum) that some are hidden for larva in the space simultaneously, larva can't be met each other, makes humidity remain on mass water content at material which can retain moisture interpolation water and reaches 50-500%.Middle and later periods the larva carrot of mainly throwing something and feeding, chrysanthemum ginger is aided with serpentgrass, the rhizome of life fruit or other plateau plant, the single feed of can throwing something and feeding, the mixed feed of also can throwing something and feeding.Make regular check on the larva living condition, a situation arises that feed, material which can retain moisture and rearing tray are changed according to larval feeding situation and disease.Raising temperature is 8-18 ℃, and lucifuge is raised; Cultivate humidity and be water breakthrough and when tilting, lose the humidity degree of flowing water.When replacing or interpolation larvae feeds, the staff must wear the fingerstall of avoiding disease to infect except conventional antiforeign bacteria pollutes dressing, touched the instrument of disease polypide or pathogen in the operating process and will in time change.
2) processing of Hunan rod bat moth group comprises intensive farm stage and wall scroll isolated rearing stage;
Being treated to of intensive farm stage wherein: 1000 of the larvas that has just hatched under drying condition are divided into 10 rent, transfer to respectively and mainly be mixed with carrot, chrysanthemum ginger, serpentgrass and life fruit, be aided with interior raising of humus soil of the rhizome of spending skin camphor tree, orangewood, oil tea, tea tree etc.The humus soil hand is firmly held can be agglomerating, and hand is decontroled the humidity standard that can not loose.The raising temperature condition is 15-25 ℃.Stocking density is that unit raises the box (amount of no more than 30 larvas of input in the long 30cm * wide 20cm height * 15cm).Since the mobility of newly hatched larvae a little less than, scope of activities is little, a little less than the injury between the individuality, so newly hatched larvae is fit to intensive farm.The about 30-40 of newly hatched larvae intensive farm days time, (a situation arises determines the concrete time according to raising temperature and disease.In 15-25 ℃ raising temperature scope, raising temperature is more low, and the time of confinement rearing is longer relatively; Disease takes place more serious, and the time of confinement rearing is shorter relatively).During this time can be according to the rotten degree of feed and how many replacings or interpolation feed and the matrix of disease.When replacing or interpolation larvae feeds or matrix, the staff must wear the fingerstall of avoiding disease to infect except conventional antiforeign bacteria pollutes dressing.In the feed process, the rhizome of carrot, chrysanthemum ginger and serpentgrass etc. cleans with ultrasonic washing instrument after cleaning with clear water again, and it is edible for newly hatched larvae to be processed into fritter then in dust proof workshop; The life fruit is cleaned the back with clear water and cleans with ultrasonic, and is directly edible for larva then.Other the rhizome as spending skin camphor tree, orangewood, oil tea, tea tree etc. all will be with that rot or too small cleaning out after first being processed or processing.Clean the larva of directly throwing something and feeding then with clear water and ultrasonic washing instrument successively.
Being treated to of wall scroll isolated rearing stage: the larva of raising in the box the about 30-40 of confinement rearing days all taken out and larva is placed wall scroll raising in the Chinese caterpillar fungus host larvae cultivation dish that embodiment 1 provides, every larva lives in independently in the space, can add some in the space simultaneously for the matrix (humus soil or artificial substratum) that larva is hidden, larva can't be met each other.Make humidity remain on mass water content at material which can retain moisture interpolation water and reach 50-500%.Middle and later periods larva mainly throw something and feed carrot, chrysanthemum ginger, serpentgrass and life fruit, be aided with the rhizome of spending skin camphor tree, orangewood, oil tea, tea tree etc., the single feed of can throwing something and feeding, the mixed feed of also can throwing something and feeding.Make regular check on the larva living condition, a situation arises that feed, material which can retain moisture and rearing tray are changed according to larval feeding situation and disease.Raising temperature is 15-25 ℃, and lucifuge is raised; Cultivate humidity and be water breakthrough and when tilting, lose the humidity degree of flowing water.When replacing or interpolation larvae feeds, the staff must wear the fingerstall of avoiding disease to infect except conventional antiforeign bacteria pollutes dressing, touched the instrument of disease polypide or pathogen in the operating process and will in time change.
3, the emergence of Chinese caterpillar fungus host larva with lay eggs
Chinese caterpillar fungus host larva all required cleanliness factor to be not less than 100,000 grades environmental condition before forming pupa; The staff requires to dress work clothes, mouth mask and cap.Middle and later periods larva with Hunan rod bat moth group and Gongga bat moth group, in the cultivation dish, grow into propupa during the stage, it is transferred in the plastic culture dish in relatively large space, be lined with fibrous material or cotton or the filter paper that can preserve moisture in the culture dish, each culture dish is thrown in a propupa.Wherein the cultivation temperature of Gongga bat moth group propupa is 8-18 ℃, and the cultivation temperature of Hunan rod bat moth group is 15-25 ℃, and lucifuge is cultivated; Cultivate humidity and be water breakthrough and when tilting, lose the humidity degree of flowing water, preserve moisture with the 60-100 ℃ of cold water of sterilizing of heating.For avoiding disease spread, should have or not the replacing fingerstall according to disease in the operating process.The pupa that will become white continues to cultivate under the same conditions, when the color transition for the treatment of pupa is pitchy, pupa is transferred in the emergence dish in the emergence chamber prepare to sprout wings.
To require cleanliness factor be to carry out under the environmental condition between 300,000 grades need not cleanliness factor in the emergence of pupa.The emergence of pupa is finished in the emergence dish.Whole emergence dish has a plurality of emergence hole, and it is fibrous material or cotton or filter paper that fixed disk 4-2 go up to place material which can retain moisture, and its humidity is water breakthrough and lose the humidity degree of flowing water when tilting, and preserves moisture with the 60-100 ℃ of cold water of sterilizing of heating.Cover a gauze 4-1 above the emergence dish, the adult that not only can prevent from sprouting wings escapes, and can also become the attachment of adult.The fully-developed pupa is broken through in the emergence dish at first from the head, slowly shelling then, and the successful adult that shells is attached to the diastole work of finishing wing on the gauze 4-1 silently.The temperature that pupa is sprouted wings is 8-25 ℃, and illumination condition is natural daylight intensity and photoperiod; The air humidity of sprouting wings is 60-70%.The adult that sprouts wings is transferred to and carries out mating in the mosquito net mating bed and lay eggs.For avoiding disease spread, should have or not the replacing fingerstall according to disease in the operating process.
Respectively in the intensive farm stage of Hunan rod bat moth group and Gongga bat moth group (the statistics first time), wall scroll isolated rearing stage (statistics for the second time) and the end back of sprouting wings (statistics for the third time) statistics Chinese caterpillar fungus host insect survival rate.Statistics finds that after finishing in the intensive farm stage, the larva of Hunan rod bat moth group has survived 931, and the larva of Gongga bat moth group has survived 964.After the wall scroll isolated rearing stage finished, the larva of Hunan rod bat moth group had survived 872, and the larva of Gongga bat moth group has survived 857.After all pupas were sprouted wings end, the larva of Hunan rod bat moth group had survived 761, and the larva of Gongga bat moth group has survived 753.Specifically as shown in table 1.
The influence of the survival rate of table 1 difference (stage) Chinese caterpillar fungus host insect
Figure BDA00003502265300061
Intensive cultivation Chinese caterpillar fungus host insect and wall scroll are isolated the comparison of cultivating the Chinese caterpillar fungus host insect in embodiment 3 conventional chamber
Realize that total man worker's cultivation of Chinese caterpillar fungus and the key that semi-wild is cultivated just are the raising cultivation of host larva, 3000 of the Gongga bat moth larvaes that employing embodiment 2 collects are divided into 3 groups at random; The called after wall scroll is isolated cultivation group I, intensive cultivation group and the wild group of putting in a suitable place to breed respectively.
Wall scroll is isolated being treated to of cultivation group I; Require to be not less than at cleanliness factor under 100,000 grades the environmental condition, place in the Chinese caterpillar fungus host larvae cultivation dish that embodiment 1 provides wall scroll to raise larva.Every larva lives in independently in the space, and larva lives on the material which can retain moisture and can't meet each other, adds water in material which can retain moisture and makes humidity remain on mass water content to reach 50-500%.Middle and later periods the larva carrot of mainly throwing something and feeding, chrysanthemum ginger is aided with serpentgrass, the rhizome of life fruit or other plateau plant, the single feed of can throwing something and feeding, the mixed feed of also can throwing something and feeding.Make regular check on the larva living condition, a situation arises that feed, material which can retain moisture and rearing tray are changed according to larval feeding situation and disease.Raising temperature is 8-18 ℃, and lucifuge is raised; Cultivate humidity and be water breakthrough and when tilting, lose the humidity degree of flowing water.When changing larvae feeds or matrix, the staff must wear the fingerstall of avoiding disease to infect except conventional antiforeign bacteria pollutes dressing, and has or not the replacing fingerstall according to disease.All grow to the propupa stage to Gongga bat moth larvae, last 7 months.
Being treated to of intensive cultivation group: require to be not less than at cleanliness factor under 100,000 grades the environmental condition, Gongga bat moth larvae is divided into 10 groups, transfers to interior raising of plateau humus soil (also available artificial matrix replaces) of a small amount of rhizome that mainly is mixed with serpentgrass, chrysanthemum ginger, carrot and life fruit and other plateau plants.Humus soil is firmly held with hand can be agglomerating, and hand is decontroled the humidity standard that can not loose.The raising temperature condition is 8-18 ℃.Stocking density is that unit raises the box (amount of no more than 30 larvas of input in the long 30cm * wide 20cm height * 15cm).Since the mobility of newly hatched larvae a little less than, scope of activities is little, the injury between the individuality can be ignored, so newly hatched larvae is fit to intensive farm.The time of the about 30-60d of newly hatched larvae intensive farm, (a situation arises determines the concrete time according to raising temperature and disease.In 8-18 ℃ raising temperature scope, raising temperature is more low, and the time of confinement rearing is longer relatively; It is more serious that disease takes place, and the time of confinement rearing is shorter relatively) after, the larva raising density unit of reducing to raises the box (amount of no more than 25 larvas of input in the long 30cm * wide 20cm height * 15cm).After raising about 60 days under this density, the larva raising density unit of reducing to raises the box (amount of no more than 20 larvas of input in the long 30cm * wide 20cm height * 15cm).After raising about 60 days under this density, the larva raising density unit of reducing to raises the box (amount of no more than 10 larvas of input in the long 30cm * wide 20cm height * 15cm).Can change or interpolation feed and matrix according to rotten degree and the having or not of disease of feed during this time.When replacing or interpolation larvae feeds or matrix, the staff must wear the fingerstall of avoiding disease to infect except conventional antiforeign bacteria pollutes dressing.In the feed process, serpentgrass, chrysanthemum ginger and carrot are cleaned with ultrasonic washing instrument after cleaning with clear water again, and it is edible for newly hatched larvae to be processed into fritter afterwards in dust proof workshop; The life fruit is cleaned the back with clear water and cleans with ultrasonic, and is directly edible for larva then.Serpentgrass, chrysanthemum ginger, carrot and life fruit all will be with that rot or too small cleaning out after first being processed or processing.All grow to the propupa stage to Gongga bat moth larvae, last 7 months.
Wildly put being treated to of group in a suitable place to breed: just Gongga bat moth larvae is transferred to CORDYCEPS summer grassland and is given birth in the environment, carries out stable breeding.Avoid cattle and sheep that stable breeding ground is trampleed in the early stage of stable breeding, avoid rodent and birds catching and killing Gongga bat moth larvae simultaneously.All grow to the propupa stage to Gongga bat moth larvae, last 18 months.
Gongga bat moth group in the comparing embodiment 2 and the intensive cultivation group among the embodiment 3, the Gongga bat moth larvae survival rate that wall scroll is isolated cultivation group I and wild cultivation group, the survival rate of finding intensive cultivation group only is 34.2%, the survival rate of wild cultivation group only is 11.4%, and the survival rate of wall scroll isolation cultivation group I is 87.1%, and the survival rate of Gongga bat moth group is 85.7%.Analyze its reason and mainly contain the following aspects:
1) the Hepialus gonggaensis larva has the person's character that cuts one another's throat.When intensive farm, a large amount of larvas concentrate in together, and this massacres each other for larva prerequisite is provided; Simultaneously, when disease takes place a certain larva, other larva of intensive farm will be infected deadly by disease owing to be co-located in the same raising box very soon.So the survival rate of intensive experiment is well below isolating the cultivation group;
2) (namely hatch back 30-60d) at the 1-2 of Gongga bat moth larvae between the length of time, the relative density of newly hatched larvae in box is less, and a little less than the mobility, the chance of meeting cannibalisation between the individuality is little; In this period, various diseases do not take place or are rare simultaneously, and the possibility of the popular propagation of disease is little; Simultaneously and since 1-2 between the length of time polypide less, adopt intensive cultivation also can save management cost.So in actual production process, before isolated rearing, namely take intensive farm can not influence the raising of larva survival rate between the length of time at the 1-2 of larva.
3) the Chinese caterpillar fungus host larvae cultivation dish that adopts of the present invention not only can effectively avoid larva (between) cut one another's throat, the mutual partition between larva also can effectively be avoided the propagation of germ; Inner bottom surface at box body 1 is provided with infiltration groove 1-1 in addition, not only can avoid occurring on the material which can retain moisture ponding, also utilizes the residual water of infiltration groove 1-1 to keep the humidity of material which can retain moisture simultaneously.

Claims (6)

1. the artificial expanding propagation method of Chinese caterpillar fungus host insect comprises ovum stage, larval stage, pupa stage and adult stage; It is characterized in that: described method is larval stage and the pupa stage of Chinese caterpillar fungus host insect to adopt wall scroll to isolate breeding method.
2. the artificial expanding propagation method of Chinese caterpillar fungus host insect according to claim 1 is characterized in that: described wall scroll is isolated the specification requirement of cultivating Chinese caterpillar fungus and is:
1) cultivating air purity requires to be not less than 100,000 grades;
2) larva is placed on mass water content on the material which can retain moisture of 50-500%, wherein flowing water flow can not occur on the material which can retain moisture.
3. the artificial expanding propagation method of Chinese caterpillar fungus host insect according to claim 2, it is characterized in that: described Chinese caterpillar fungus host insect is Lepidoptera Lepidoptera Hepialidae Hepialidae insect.
4. the artificial expanding propagation method of Chinese caterpillar fungus host insect according to claim 3, it is characterized in that: the temperature of described cultivation is 8-25 ℃.
5. the Chinese caterpillar fungus host insect is artificial expands numerous special equipment, it is characterized in that: described equipment arranges the box body (1), partition apparatus (2), material which can retain moisture of infiltration groove (1-1) by a bottom and forms with lid (3) four parts that partition apparatus is complementary; Described material which can retain moisture is arranged on the bottom of box body (1), and partition apparatus (2) arranges on the material which can retain moisture, and lid (3) covers partition apparatus (2) top.
6. Chinese caterpillar fungus host insect according to claim 5 is manually expanded numerous special equipment, it is characterized in that: the numerous special equipment of the artificial expansion of described Chinese caterpillar fungus host insect has the material of black or other dark lucifuges to make.
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CN104285912A (en) * 2014-10-23 2015-01-21 中国农业科学院蔬菜花卉研究所 Indoor fast spider mite population propagation method
CN104585138B (en) * 2015-01-07 2017-01-25 杨琴 Soil layered rearing method for swift moth larvae
CN104585138A (en) * 2015-01-07 2015-05-06 杨琴 Soil layered rearing method for swift moth larvae
CN105766379B (en) * 2016-03-22 2017-05-03 广东东阳光药业有限公司 System and method for cultivating caterpillar fungus
CN105766379A (en) * 2016-03-22 2016-07-20 广东东阳光药业有限公司 System and method for cultivating caterpillar fungus
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CN106228457A (en) * 2016-07-27 2016-12-14 重庆市中药研究院 Unhealthy larva screening system and method
CN106719466A (en) * 2017-03-31 2017-05-31 广东东阳光药业有限公司 The pupa moth integrated nursing care device and care method of bat moth
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CN107549120A (en) * 2017-09-27 2018-01-09 湖南师范大学 The rearing method and its larvae feeds formula of a kind of bat moth
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CN115067281A (en) * 2022-06-13 2022-09-20 云南省林业和草原科学院 Method for interfering egg laying behavior of citrus fruit fly adults by using sound

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