CN102283177A - Method for recycling and treating feeding matrix of aweto parasitic larva - Google Patents

Method for recycling and treating feeding matrix of aweto parasitic larva Download PDF

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Publication number
CN102283177A
CN102283177A CN2011102063213A CN201110206321A CN102283177A CN 102283177 A CN102283177 A CN 102283177A CN 2011102063213 A CN2011102063213 A CN 2011102063213A CN 201110206321 A CN201110206321 A CN 201110206321A CN 102283177 A CN102283177 A CN 102283177A
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matrix
hours
temperature
cordyceps sinensis
dry
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CN2011102063213A
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CN102283177B (en
Inventor
陈仕江
尹定华
张德利
曾纬
涂永勤
刘飞
李黎
马开森
钟国跃
罗庆明
钱敏
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Chongqing Academy of Chinese Materia Medica
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Chongqing Academy of Chinese Materia Medica
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Priority to CN 201110206321 priority Critical patent/CN102283177B/en
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Publication of CN102283177B publication Critical patent/CN102283177B/en
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  • Mushroom Cultivation (AREA)

Abstract

The invention provides a method for recycling and treating a feeding matrix of an aweto parasitic larva by ultraviolet sterilizing, disinfecting and moisturizing again. The matrix processed by the processing method can be directly used for feeding the aweto parasitic larva, thus the recycling of the matrix is realized. The expense for treating the matrix by using the treating method disclosed by the invention is lower than that for re-preparing the matrix; and more importantly, the damage to alpine meadow is reduced.

Description

The recycling processing method of Cordyceps sinensis host larva raising matrix
Technical field
The present invention relates to a kind of caterpillar fungus cultivation technology, relate to the recycling processing method of a kind of Cordyceps sinensis host larva raising matrix in particular.
Technical background
Cordyceps sinensis, be that the section ergot fungus cordyceps sinensis bacterium colonizes in the stroma that forms on the Hepialidae insect larvae body and the complex of larva corpse, be a kind of traditional famous and precious tonic Chinese herbal medicine material, multiple efficacies such as regulating function of immune system, antitumor, antifatigue is arranged.The upper epidermis that the used high-quality matrix of host's larva raising is alpine meadow soil forms through processing and fabricating.Alpine meadow soil has tangible humus to gather, 8~20 centimetres of humus bed thickness, be greyish brown to pitchy granular-flat nutty structure.The content of organic matter 10~20%, based on fulvic acid, humic acid/fulvic acid (H/F) ratio is 0.6~1.0.The soil composite colloid belongs to the low multiplicity type of high organic, closes the attitude humus based on untwisting.The downward color of humus layer is thin out rapidly.But only 40~50 centimetres of alpine meadow soil thickness have tangible thawing micromorphological features, bottom have freeze season the layer or ever-frozen ground.The formation cycle is longer, in case large-scale destruction recovered very difficulty, can cause occurring the situation of plateau desertification.
Summary of the invention
At present in the artificial culture of Cordyceps sinensis,, because through after once using, have parasitic wasp in the matrix as Cordyceps sinensis host larva raising matrix, very insect such as living fly lays eggs in matrix in a large number; Produce a large amount of assorted bacterium simultaneously in the matrix, cause matrix not reuse once more, adopt conventional medicine dissipation or high temperature dissipation method all can cause matrix not reuse.The invention provides the recycling processing method of a kind of Cordyceps sinensis host larva raising matrix for addressing the above problem, may further comprise the steps:
1) used matrix is placed sunlight canopy environment thermal source, temperature adjustable, the fluffy thickness 2~5cm that is tiled into, 60~65 ℃ of heating-up temperatures, UV intensity of illumination 3000~4000Lux, dry 5~8 hours;
2) the matrix lucifuge in described with step 1), 10~13 ℃ of temperature were placed 24~72 hours;
3) repeating step 1) and step 2) be operated to matrix dehydration, serve as dehydration 80 ± 2% with the dry weight water content meter, do not have survival parasitic wasp, fly larva and ovum and part pathogeny bacterium, obtain dry matrices;
4) with the described dry matrices of step 3), cross 30 mesh sieves, obtain powdery matrix;
5) powdery matrix is added water and preserve moisture,, kept humidity 12 hours to being moisture 40~60% with the dry weight water content meter;
6) the powdery matrix of step 5) being preserved moisture after handling seals, and 60~80 ℃ of sterilizations obtained finished product in 3~5 hours.
The described matrix of step 1) is the upper epidermis of the above alpine meadow soil of height above sea level 2000m, pulverizes, sieves, and is prepared from.
The described heating-up temperature of step 1) is 60~65 ℃; Step 2) 10~13 ℃ of the temperature described in.
Useful technique effect of the present invention is: the matrix that adopts disposal methods of the present invention to cross can be directly used in Cordyceps sinensis host larva raising, realized the recycling of matrix, the expense of disposal methods matrix of the present invention is lower than the expense for preparing matrix again, the more important thing is the destruction of having reduced alpine meadow.
Embodiment
The place of carrying out the recycling processing of Cordyceps sinensis host larva raising matrix is typically chosen in the region of height above sea level dry and rainless more than 2500 meters, and the place should have the facility of moisture proof of taking shelter from rain and heating.
Used matrix is tiled in the enclosure space that is provided with the underground heat facility, the fluffy thickness 2~5cm that is tiled into, optimal thickness is 3cm, 60~65 ℃ of heating-up temperatures, UV intensity of illumination 3000~4000Lux, dry sterilization 6 hours; Be cooled to 10~13 ℃ of cooling processing then 24~72 hours, and repeated aforesaid operations 3 times, the parasitic wasp in matrix, very larva and the ovum of insect such as living fly are eliminated substantially; The protection against the tide of taking shelter from rain in the whole process, reaching with the dry weight water content meter is dehydration 80 ± 2%.
The matrix of handling is sieved with 30 purpose sieve apertures, mainly remove the foreign material harmful such as feed block remaining, that rot and weeds coring larva raising.To add water and preserve moisture through previous processed matrix later then, make its humidity reach 40%~60% back and stacked 12 hours, to the dry weight water content meter be moisture 40~60%.
At last matrix is packed in the plastic sack of 25 * 30cm, envelope ties sack; Putting into large-scale water-bath disinfects.Sterilization thermal source underground heat thermal water, water temperature remains in 60~65 ℃ of scopes and promptly reaches disinfective action after 3~5 hours, takes out promptly to can be used for going breeding grub again.
By the matrix that the method for the invention is handled out, the content of organic matter is stable, and humic acid/fulvic acid (H/F) ratio is 0.6~1.0 for appointing.Be suitable as Cordyceps sinensis host larva raising matrix.

Claims (3)

1. the recycling processing method of Cordyceps sinensis host larva raising matrix is characterized in that may further comprise the steps:
1) used matrix is placed sunlight canopy environment thermal source, temperature adjustable, the fluffy thickness 2~5cm that is tiled into, 60~65 ℃ of heating-up temperatures, UV intensity of illumination 3000~4000Lux, dry 5~8 hours;
2) the matrix lucifuge in described with step 1), 10~13 ℃ of temperature were placed 24~72 hours;
3) repeating step 1) and step 2) be operated to matrix dehydration, serve as dehydration 80 ± 2% with the dry weight water content meter, do not have survival parasitic wasp, fly larva and ovum, obtain dry matrices;
4) with the described dry matrices of step 3), cross 30 mesh sieves, obtain powdery matrix;
5) powdery matrix is added water and preserve moisture,, kept humidity 12 hours to being moisture 40~60% with the dry weight water content meter;
6) the powdery matrix of step 5) being preserved moisture after handling seals, and 60~80 ℃ of sterilizations obtained finished product in 3~5 hours.
2. the recycling processing method of Cordyceps sinensis host larva raising matrix according to claim 1, it is characterized in that: the described matrix of step 1) is the upper epidermis of the above alpine meadow soil of height above sea level 2000m, pulverizes, sieves, and is prepared from.
3. the recycling processing method of Cordyceps sinensis host larva raising matrix according to claim 1, it is characterized in that: the described heating-up temperature of step 1) is 60~65 ℃; Step 2) 10~13 ℃ of the temperature described in.
CN 201110206321 2011-07-22 2011-07-22 Method for recycling and treating feeding matrix of aweto parasitic larva Expired - Fee Related CN102283177B (en)

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CN102283177B CN102283177B (en) 2013-04-24

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103299963A (en) * 2013-07-12 2013-09-18 重庆市中药研究院 Manual expanding propagation method and special equipment thereof for worm grass host insects
CN103548780A (en) * 2013-11-15 2014-02-05 重庆市中药研究院 Method for preventing and controlling paecilomyces disease in artificially breeding ophiocordyceps sinensis larvae host process
CN104226670A (en) * 2013-06-21 2014-12-24 中国农业科学院植物保护研究所 Treatment and reutilization method for planting matrixes of host plants in manual and mass production of juice-absorbing injurious insects and natural enemies thereof
CN112586454A (en) * 2020-12-15 2021-04-02 青海珠峰冬虫夏草工程技术研究有限公司 Large-scale breeding method for cordyceps sinensis host hepialus armoricanus larvae

Citations (4)

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CN1297774A (en) * 1999-12-01 2001-06-06 上海国宝企业发展中心 Extraction of effective component in Northern cordyceps producing matrix
KR20040076506A (en) * 2003-02-26 2004-09-01 양사헌 Method for producing cordyceps sinensis using larvaes of fly
JP2004267004A (en) * 2003-03-04 2004-09-30 Oubiken:Kk Method for culturing cordyceps sinensis
CN101648029A (en) * 2008-08-14 2010-02-17 上海国宝企业发展中心 Method for curing and sterilizing culture media for large-scale cultivation of cordyceps militaris

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1297774A (en) * 1999-12-01 2001-06-06 上海国宝企业发展中心 Extraction of effective component in Northern cordyceps producing matrix
KR20040076506A (en) * 2003-02-26 2004-09-01 양사헌 Method for producing cordyceps sinensis using larvaes of fly
JP2004267004A (en) * 2003-03-04 2004-09-30 Oubiken:Kk Method for culturing cordyceps sinensis
CN101648029A (en) * 2008-08-14 2010-02-17 上海国宝企业发展中心 Method for curing and sterilizing culture media for large-scale cultivation of cordyceps militaris

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Title
刘飞等: "冬虫夏草寄主幼虫人工饲养初期的成活率研究", 《重庆中草药研究》 *
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104226670A (en) * 2013-06-21 2014-12-24 中国农业科学院植物保护研究所 Treatment and reutilization method for planting matrixes of host plants in manual and mass production of juice-absorbing injurious insects and natural enemies thereof
CN103299963A (en) * 2013-07-12 2013-09-18 重庆市中药研究院 Manual expanding propagation method and special equipment thereof for worm grass host insects
CN103299963B (en) * 2013-07-12 2016-01-20 重庆市中药研究院 The mass rearing method of Chinese caterpillar fungus host insect and special equipment thereof
CN103548780A (en) * 2013-11-15 2014-02-05 重庆市中药研究院 Method for preventing and controlling paecilomyces disease in artificially breeding ophiocordyceps sinensis larvae host process
CN103548780B (en) * 2013-11-15 2016-01-13 重庆市中药研究院 The host larva Paecilomyces varioti disease preventing control method of artificial feeding Cordyceps sinensis
CN112586454A (en) * 2020-12-15 2021-04-02 青海珠峰冬虫夏草工程技术研究有限公司 Large-scale breeding method for cordyceps sinensis host hepialus armoricanus larvae

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