CN103237809B - 一个集合及其使用方法 - Google Patents

一个集合及其使用方法 Download PDF

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Publication number
CN103237809B
CN103237809B CN201180055462.8A CN201180055462A CN103237809B CN 103237809 B CN103237809 B CN 103237809B CN 201180055462 A CN201180055462 A CN 201180055462A CN 103237809 B CN103237809 B CN 103237809B
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seq
antibodies
germline
pairs
light chain
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Chinese (zh)
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CN103237809A (zh
Inventor
斯特法妮·尤林格
托马斯·蒂勒
英格里德·舒斯特
伊温妮·斯塔克
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Morphosys AG
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Morphosys AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201180055462.8A 2010-11-19 2011-11-18 一个集合及其使用方法 Active CN103237809B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US41536710P 2010-11-19 2010-11-19
EP10191910 2010-11-19
EP10191910.8 2010-11-19
US61/415,367 2010-11-19
US201161494452P 2011-06-08 2011-06-08
US61/494,452 2011-06-08
PCT/EP2011/070473 WO2012066129A1 (en) 2010-11-19 2011-11-18 A collection and methods for its use

Publications (2)

Publication Number Publication Date
CN103237809A CN103237809A (zh) 2013-08-07
CN103237809B true CN103237809B (zh) 2016-09-07

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US (3) US8728981B2 (cg-RX-API-DMAC7.html)
EP (1) EP2640742B1 (cg-RX-API-DMAC7.html)
JP (1) JP6253986B2 (cg-RX-API-DMAC7.html)
KR (1) KR101938021B1 (cg-RX-API-DMAC7.html)
CN (1) CN103237809B (cg-RX-API-DMAC7.html)
AU (1) AU2011331129B2 (cg-RX-API-DMAC7.html)
CA (1) CA2816558C (cg-RX-API-DMAC7.html)
DK (1) DK2640742T3 (cg-RX-API-DMAC7.html)
ES (1) ES2696548T3 (cg-RX-API-DMAC7.html)
HR (1) HRP20181811T1 (cg-RX-API-DMAC7.html)
IL (1) IL226364B (cg-RX-API-DMAC7.html)
MX (1) MX353795B (cg-RX-API-DMAC7.html)
NZ (1) NZ609707A (cg-RX-API-DMAC7.html)
PL (1) PL2640742T3 (cg-RX-API-DMAC7.html)
RU (1) RU2603080C2 (cg-RX-API-DMAC7.html)
SG (2) SG10201509257VA (cg-RX-API-DMAC7.html)
WO (1) WO2012066129A1 (cg-RX-API-DMAC7.html)
ZA (1) ZA201303566B (cg-RX-API-DMAC7.html)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102993305B (zh) * 2012-11-16 2015-05-13 上海赛伦生物技术有限公司 人源抗人表皮生长因子受体抗体及其编码基因与应用
LT3046412T (lt) * 2013-09-18 2019-07-25 Regeneron Pharmaceuticals, Inc. Histidino pakeitimus lengvoje grandinėje turintys antikūnai ir genetiškai modifikuoti gyvūnai, išskyrus žmogų, išvesti jų gamybai
US10684289B2 (en) 2013-12-09 2020-06-16 Adimab, Llc Polyclonal mixtures of antibodies, and methods of making and using them
BR112016014824A2 (pt) * 2013-12-27 2017-09-19 Chugai Pharmaceutical Co Ltd Método para purificar anticorpo que tem ponto isoelétrico baixo
MA44227B1 (fr) 2016-02-19 2021-06-30 Galapagos Nv Anticorps pour il-17c
EP3443087B1 (en) * 2016-04-12 2024-09-18 Medimmune, LLC Immune repertoire mining
WO2018087349A1 (en) 2016-11-14 2018-05-17 Morphosys Ag Fab molecules with a rodent hinge region and a non-rodent ch1 region
WO2019099454A2 (en) 2017-11-15 2019-05-23 Philippe Valadon Highly functional antibody libraries
IL278323B2 (en) 2018-05-16 2025-05-01 Morphosys Ag Antibodies directed against glycoprotein IV
KR102115300B1 (ko) 2018-06-01 2020-05-26 재단법인 목암생명과학연구소 항체 라이브러리 및 이를 이용한 항체 스크리닝 방법
US20230183675A1 (en) 2020-05-20 2023-06-15 Institut Curie Synthetic Single Domain Library
WO2021250017A1 (en) 2020-06-08 2021-12-16 Immundiagnostik Ag Improved method and test system for in-vitro determination of drug antibodies in blood
EP4217391A1 (en) 2020-09-24 2023-08-02 MorphoSys AG Novel human antibodies binding to human cd3 epsilon
JP2025513714A (ja) 2022-03-22 2025-04-30 モルフォシス・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Ｃｄ３に特異的な脱免疫抗体
WO2024236163A1 (en) 2023-05-17 2024-11-21 Morphosys Ag T cell receptor beta constant region 2 (trbc2) antibodies
WO2025003511A1 (en) 2023-06-30 2025-01-02 Morphosys Ag Dual-targeting of flt3 and cd123 co-expressing tumor cells by functional complementation of cycat® halfbody molecules
WO2025082777A1 (en) 2023-10-17 2025-04-24 Morphosys Ag Dual-targeting of muc16 and mesothelin co-expressing tumor cells by functional complementation of cycat® halfbody molecules

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009036379A2 (en) * 2007-09-14 2009-03-19 Adimab, Inc. Rationally designed, synthetic antibody libraries and uses therefor

Family Cites Families (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618920A (en) 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5258498A (en) 1987-05-21 1993-11-02 Creative Biomolecules, Inc. Polypeptide linkers for production of biosynthetic proteins
US5091513A (en) 1987-05-21 1992-02-25 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
DE3853515T3 (de) 1987-05-21 2005-08-25 Micromet Ag Multifunktionelle proteine mit vorbestimmter zielsetzung.
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
DE68927933T2 (de) 1988-09-02 1997-08-14 Dyax Corp Herstellung und auswahl von rekombinantproteinen mit verschiedenen bindestellen
ATE102631T1 (de) 1988-11-11 1994-03-15 Medical Res Council Klonierung von immunglobulin sequenzen aus den variabelen domaenen.
US6291161B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
US6291158B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertoire
CA2016841C (en) 1989-05-16 1999-09-21 William D. Huse A method for producing polymers having a preselected activity
CA2057923A1 (en) 1989-05-16 1990-11-17 William D. Huse Co-expression of heteromeric receptors
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US6291160B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US6291159B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US5859205A (en) 1989-12-21 1999-01-12 Celltech Limited Humanised antibodies
AU7247191A (en) 1990-01-11 1991-08-05 Molecular Affinities Corporation Production of antibodies using gene libraries
US5780225A (en) 1990-01-12 1998-07-14 Stratagene Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
DE4002897A1 (de) 1990-02-01 1991-08-08 Behringwerke Ag Herstellung und verwendung von genbanken synthetischer menschlicher antikoerper ("synthetische human-antikoerper-bibliotheken")
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
EP0585287B1 (en) 1990-07-10 1999-10-13 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
AU1545692A (en) 1991-03-01 1992-10-06 Protein Engineering Corporation Process for the development of binding mini-proteins
WO1992015678A1 (en) 1991-03-01 1992-09-17 Stratagene Pcr generated dicistronic dna molecules for producing antibodies
ATE175997T1 (de) 1991-08-10 1999-02-15 Medical Res Council Behandlung von zellpopulationen
EP0605522B1 (en) 1991-09-23 1999-06-23 Medical Research Council Methods for the production of humanized antibodies
US5565332A (en) 1991-09-23 1996-10-15 Medical Research Council Production of chimeric antibodies - a combinatorial approach
WO1993011236A1 (en) 1991-12-02 1993-06-10 Medical Research Council Production of anti-self antibodies from antibody segment repertoires and displayed on phage
US5667988A (en) 1992-01-27 1997-09-16 The Scripps Research Institute Methods for producing antibody libraries using universal or randomized immunoglobulin light chains
US5395750A (en) 1992-02-28 1995-03-07 Hoffmann-La Roche Inc. Methods for producing proteins which bind to predetermined antigens
CA2131151A1 (en) 1992-03-24 1994-09-30 Kevin S. Johnson Methods for producing members of specific binding pairs
US5855885A (en) 1993-01-22 1999-01-05 Smith; Rodger Isolation and production of catalytic antibodies using phage technology
AU8091694A (en) 1993-10-26 1995-05-22 United Biomedical Inc. Structured synthetic antigen libraries as diagnostics, vaccines and therapeutics
US20010049107A1 (en) 1994-01-31 2001-12-06 Boston University Polyclonal antibody libraries
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5977322A (en) 1995-06-14 1999-11-02 The Regents Of The University Of California High affinity human antibodies to tumor antigens
US6828422B1 (en) 1995-08-18 2004-12-07 Morphosys Ag Protein/(poly)peptide libraries
EP0859841B1 (en) 1995-08-18 2002-06-19 MorphoSys AG Protein/(poly)peptide libraries
GB9603507D0 (en) 1996-02-20 1996-04-17 Isis Innovation Antibody variants
CA2297070A1 (en) 1997-08-01 1999-02-11 Morphosys Ag Novel method and phage for the identification of nucleic acid sequences encoding members of a multimeric (poly)peptide complex
GB9722131D0 (en) 1997-10-20 1997-12-17 Medical Res Council Method
ATE296839T1 (de) 1999-03-01 2005-06-15 Genentech Inc Antikörper zur krebsbehandlung und -diagnose
IL142025A0 (en) 1999-07-20 2002-03-10 Morphosys Ag Novel methods for displaying (poly) peptides/proteins on bacteriophage particles via disulfide bonds
IL136459A0 (en) 2000-05-30 2001-06-14 Galim Galil Immunology Ltd Antibody library
JP4860098B2 (ja) 2000-12-18 2012-01-25 ダイアックス、コープ 遺伝的パッケージの焦点を合わせたライブラリー
US7117096B2 (en) 2001-04-17 2006-10-03 Abmaxis, Inc. Structure-based selection and affinity maturation of antibody library
US20030186374A1 (en) 2001-10-01 2003-10-02 Hufton Simon E. Multi-chain eukaryotic display vectors and uses thereof
GB0130267D0 (en) 2001-12-19 2002-02-06 Neutec Pharma Plc Focussed antibody technology
AU2002360068B2 (en) 2001-12-21 2009-09-03 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Method for cloning of variable domain sequences
US7244592B2 (en) 2002-03-07 2007-07-17 Dyax Corp. Ligand screening and discovery
US8206977B2 (en) 2002-07-30 2012-06-26 Morphosys Ag Tricistronic vectors and uses therefor
US8557743B2 (en) 2002-09-05 2013-10-15 Dyax Corp. Display library process
GB0309126D0 (en) 2003-04-17 2003-05-28 Neutec Pharma Plc Clostridium difficile focussed antibodies
WO2005023993A2 (en) 2003-09-09 2005-03-17 Integrigen, Inc. Methods and compositions for generation of germline human antibody genes
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
NZ547260A (en) 2003-11-12 2009-07-31 Patrys Ltd Methods of identifying neoplasm-specific antibodies and uses thereof
US20080003566A1 (en) 2004-01-26 2008-01-03 David Vaux Molecular Analysis
ATE505727T1 (de) 2004-05-26 2011-04-15 Fraunhofer Ges Forschung Isolation allergen-spezifischer immunoglobulin- gene aus humanen b-zellen von atopikern
EP1781680B8 (en) 2004-07-06 2016-05-18 Bioren, LLC Universal antibody libraries
US7977050B2 (en) 2004-09-02 2011-07-12 Bioarray Solutions, Ltd. Nucleic acid amplification with integrated multiplex detection
US20060078898A1 (en) 2004-10-12 2006-04-13 Curry Bo U Methods and compositions for reducing label variation in array-based comparative genome hybridization assays II
US20080003617A1 (en) * 2004-12-20 2008-01-03 Xiaomin Fan Methods for the identification and the isolation of epitope specific antibodies
WO2006084050A2 (en) 2005-02-01 2006-08-10 Dyax Corp. Libraries and methods for isolating antibodies
RU2311927C2 (ru) * 2005-08-15 2007-12-10 Федеральное государственное унитарное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека" КОМБИНАТОРНАЯ ФАГМИДНАЯ БИБЛИОТЕКА ОДНОЦЕПОЧЕЧНЫХ АНТИТЕЛ ЧЕЛОВЕКА, ОБОГАЩЕННАЯ АНТИТЕЛАМИ ПРОТИВ ВИРУСА ОСПОВАКЦИНЫ, РЕКОМБИНАНТНАЯ ФАГМИДНАЯ ДНК pHEN-2A8, СОДЕРЖАЩАЯ УНИКАЛЬНЫЙ ГЕН ОДНОЦЕПОЧЕЧНОГО АНТИТЕЛА ЧЕЛОВЕКА, СПОСОБНОГО НЕЙТРАЛИЗОВАТЬ ВИРУС ОСПОВАКЦИНЫ И ВИРУС ОСПЫ КОРОВ, И ИСКУССТВЕННОЕ ОДНОЦЕПОЧЕЧНОЕ АНТИТЕЛО ЧЕЛОВЕКА 2A8, СПОСОБНОЕ НЕЙТРАЛИЗОВАТЬ ВИРУС ОСПОВАКЦИНЫ И ВИРУС ОСПЫ КОРОВ
EP2465870A1 (en) 2005-11-07 2012-06-20 Genentech, Inc. Binding polypeptides with diversified and consensus VH/VL hypervariable sequences
EP1979378B1 (en) 2005-12-20 2012-07-25 MorphoSys AG Novel collection of hcdr3 regions and uses therefor
EP2021795A4 (en) 2006-05-04 2009-05-13 Abmaxis Inc CROSS SPECIES AND MULTI-SPECIAL PRESENTATION SYSTEMS
RU2010102859A (ru) 2007-08-21 2011-09-27 Морфосис Аг (De) Улучшенные способы образования дисульфидных связей
CA2710373A1 (en) 2007-12-19 2009-07-09 Ping Tsui Design and generation of human de novo pix phage display libraries via fusion to pix or pvii, vectors, antibodies and methods
EP2088432A1 (en) 2008-02-11 2009-08-12 MorphoSys AG Methods for identification of an antibody or a target
EP2100970B1 (en) 2008-03-13 2017-05-10 National Institute of Immunology Ig genes specific oligonucleotides and uses thereof
WO2009114815A1 (en) 2008-03-13 2009-09-17 Dyax Corp Libraries of genetic packages comprising novel hc cdr3 designs
EP2331570B1 (en) 2008-09-10 2014-08-27 Philochem AG Display library for antibody selection
EP2356270B1 (en) 2008-11-07 2016-08-24 Fabrus Llc Combinatorial antibody libraries and uses thereof
WO2010130824A2 (en) * 2009-05-15 2010-11-18 Morphosys Ag Collections and uses thereof
CN102449149B (zh) * 2009-05-29 2014-04-23 莫佛塞斯公司 集合及其使用方法
US9650432B2 (en) 2010-01-29 2017-05-16 Morphosys Ag Rodent combinatorial antibody libraries

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009036379A2 (en) * 2007-09-14 2009-03-19 Adimab, Inc. Rationally designed, synthetic antibody libraries and uses therefor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Biophysical Properties of Human Antibody Variable Domains;Stefan Ewert，et al.;《J. Mol. Biol.》;20030117;第325卷(第3期);第531-553页 *
Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering;Stefan Ewert，et al.;《Methods》;20041031;第34卷(第2期);第184–199页 *

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AU2011331129B2 (en) 2016-10-06
MX2013005614A (es) 2013-11-21
PL2640742T3 (pl) 2019-01-31
DK2640742T3 (en) 2018-12-03
US8367586B2 (en) 2013-02-05
EP2640742A1 (en) 2013-09-25
CA2816558A1 (en) 2012-05-24
RU2013126025A (ru) 2014-12-27
NZ609707A (en) 2014-12-24
US20120129717A1 (en) 2012-05-24
CA2816558C (en) 2020-12-29
US20130236902A1 (en) 2013-09-12
JP2014500725A (ja) 2014-01-16
IL226364B (en) 2020-11-30
WO2012066129A1 (en) 2012-05-24
US20140206575A1 (en) 2014-07-24
KR20140015274A (ko) 2014-02-06
US9541559B2 (en) 2017-01-10
ZA201303566B (en) 2014-07-30
EP2640742B1 (en) 2018-08-15
US8728981B2 (en) 2014-05-20
KR101938021B1 (ko) 2019-01-11
RU2603080C2 (ru) 2016-11-20
SG189950A1 (en) 2013-06-28
CN103237809A (zh) 2013-08-07
JP6253986B2 (ja) 2017-12-27
SG10201509257VA (en) 2015-12-30
AU2011331129A1 (en) 2013-05-23
MX353795B (es) 2018-01-30
IL226364A0 (en) 2013-07-31
HRP20181811T1 (hr) 2018-12-28
ES2696548T3 (es) 2019-01-16

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