CN103190450A - Application of cynanchum glaucescens extract in controlling psylla chinensis - Google Patents
Application of cynanchum glaucescens extract in controlling psylla chinensis Download PDFInfo
- Publication number
- CN103190450A CN103190450A CN2013101534726A CN201310153472A CN103190450A CN 103190450 A CN103190450 A CN 103190450A CN 2013101534726 A CN2013101534726 A CN 2013101534726A CN 201310153472 A CN201310153472 A CN 201310153472A CN 103190450 A CN103190450 A CN 103190450A
- Authority
- CN
- China
- Prior art keywords
- extract
- cynanchum glaucescens
- pear sucker
- application according
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to an application of a cynanchum glaucescens extract in controlling psylla chinensis, belonging to the field of biological control of plant sources. Control effects of cynanchum glaucescens extract water aqua on overwintering psylla chinensis adults are preliminarily studied by using indoor toxicity measurement and field trial methods. Researches show that the cynanchum glaucescens extract water aqua in different concentrations has remarkable lethal effect on the overwintering psylla chinensis adults; after the overwintering psylla chinensis adults are treated for 24 hours, the field control effect of 0.07mg/mL of cynanchum glaucescens extract water aqua on psylla chinensis adults reaches 66.8%, and the indoor biological measurement effect reaches 100%; and the concentration of a formula is positively associated with the lethal effect on the overwintering psylla chinensis adults. Research results provide a new green control medicament and technology for control of the overwintering psylla chinensis adults in pear orchard.
Description
Technical field
The present invention relates to the method for biological control pear sucker, particularly relate to the application of Rhizoma Cynanchi Stauntonii extract in the control pear sucker.
Background technology
China pear sucker Psylla chinensis Yang et Li, belong to the Homoptera Psyllidae, be also referred to as pear sucker, distribute very extensive in the most of area of China, it is one of topmost insect in the theatre, in recent years in North China, northeast even theatre, the south of the lower reaches of the Yangtze River take place seriously, becomes one of key factor that influence the operatic circle production.Existing method for integrated control is generally taked:
(1) the dry branches and fallen leaves weeds of tree are namely thoroughly removed in cultural control, scrape bark from old tress, severe winter and water and freeze water, can reduce adult partial wintering, play certain preventive and therapeutic effect, but act on limited.
(2) the Sheng phase chemical control of coming out of hibernation, namely spray 1500～2000 times of liquid of pyrethroid chemical agent in adult Sheng phase of coming out of hibernation of surviving the winter mid-March, the control adult radix of coming out of hibernation, it is obvious to reduce the worm amount effect of surviving the winter, but also killed natural enemy when chemical pesticide kills off the insect pests, destroyed the orchard ecology balance; On the other hand, in organic theatre, forbid using the pyrethroid chemical pesticide, plant insecticide is allowed to use.
(3) fallen flowers phase chemical control, namely at pears fallen flowers about 95%, namely the-concentrate the incubation period most critical period of pear sucker control just for nymph.Select 4000～6000 times of liquid of 10% Imidacloprid for use, 1.8% likes clear (acetamiprid) 2000～4000 times of liquid of promise worm, 3.2% Avermectin (No. 4) 5000-8000 doubly waits medicament and concentration, serious theatre takes place, can be under above-mentioned medicament and concentration, add to help and kill or clear up 1000 times of liquid of spirit, auxiliary agents such as organosilicon, to improve drug effect, can obviously reduce orchard pear sucker population quantity, alleviate and cause harm, but this phase also is the peak period of theatre natural enemy insect, using of chemical pesticide killed and wounded a large amount of orchard natural enemies, can cause the rampant again of theatre insect; On the other hand, in organic theatre, forbidding using all kinds of chemical pesticides, must find the control of insect measure that can substitute, is class environmental friendliness medicament efficiently as plant insecticide.
Studies show that the control of overwinter generation and first generation adult is effectively to control the critical period that pear sucker is caused harm.In order to reduce the control of insect means to the lethal effect of orchard natural enemy, keep the orchard ecology balance, adapt to the development need in organic theatre, plant insecticide becomes one of important measures of effective biological control, also is the method for preventing and treating of more economical more environmental protection.
Summary of the invention
At the defective in the above-mentioned field, the invention provides the application technology of a kind of Rhizoma Cynanchi Stauntonii extract in the control pear sucker, has application promise in clinical practice at sustainable control pear sucker aspect causing harm, for the control of organic theatre sucking pest from now on provides new technology, has theory and practice meaning preferably.
The application of Rhizoma Cynanchi Stauntonii extract in the control pear sucker.
Described being applied as made water formulation with Rhizoma Cynanchi Stauntonii extract and killed pear sucker.
Described being applied as made the pear tree that the water formulation sprinkling is caused harm by pear sucker with Rhizoma Cynanchi Stauntonii extract.
Described pear sucker is overwinter generation pear sucker adult.
The dosage of described sprinkling is 1m
2The required Rhizoma Cynanchi Stauntonii extract amount of dry matter of fruit tree area is 0.0250g-0.0438g.
Described Rhizoma Cynanchi Stauntonii extract is the Alcoholic extraction thing of Cynanchum glaucescens.
Described extract is medicinal extract extract, crude extract or essence extract.
Described medicinal extract preparation method of extract is: Cynanchum glaucescens plant under ground portion is cleaned, dried in the shade, pulverize, at room temperature, hydrochloric acid industrial alcohol lixiviate plant powder with 0.1%, filter, filtrate decompression concentrate medicinal extract, filter residue continues the hydrochloric acid industrial alcohol lixiviate with 0.1%, so repeatedly, merge each time and get the medicinal extract extract;
The preparation method of described crude extract is: with the medicinal extract extract with 2% dissolve with hydrochloric acid solution, remove by filter insoluble matter, acid liquid is removed impurity with chloroform extraction, and sour water is transferred pH9-10 with sodium hydroxide, use chloroform extraction 3-5 time more successively, concentrated chloroform mutually Mongolian Cynanchum glaucescens crude extract;
The preparation method of described essence extract is: with crude extract with 2% dissolve with hydrochloric acid solution, remove by filter insoluble matter, acid liquid is removed impurity with chloroform extraction, sour water is transferred pH9-10 with sodium hydroxide, use chloroform extraction 3-5 time more successively, concentrated chloroform mutually Mongolian Cynanchum glaucescens crude extract, 2-3 time repeatedly, obtain the Cynanchum glaucescens essence extract.
Described Cynanchum glaucescens is Mongolian Cynanchum glaucescens.
The present invention adopts Toxicity Determination and field trial method, Primary Study the control effect of Rhizoma Cynanchi Stauntonii extract aqua to overwinter generation pear sucker adult.Discover that the Rhizoma Cynanchi Stauntonii extract aqua of variable concentrations has significant lethal effect to overwinter generation pear sucker adult; Handle back 24h, 0.07mg/mL Cynanchum glaucescens aqua reaches 66.8% to the field control effect of pear sucker adult, and the indoor bioassay effect reaches 100%, and the lethal effect of formulation concentrations and overwinter generation pear sucker adult is proportionate.
Result of study is for the control of theatre overwinter generation pear sucker adult provides new green prevention and control medicament and technology.
Description of drawings
Fig. 1 Mongolia Rhizoma Cynanchi Stauntonii extract aqua is to overwinter generation pear sucker adult 24h Toxicity Determination,
Fig. 2 Mongolia Rhizoma Cynanchi Stauntonii extract aqua is to the field efficacy of pear sucker adult,
Fig. 3 different regions overwinter generation pear sucker adult is to the susceptibility of Cynanchum glaucescens aqua.
Embodiment
The present invention is described in further detail below in conjunction with embodiment.
1 materials and methods
1.1 material
The overwinter generation pear sucker picks up from Beijing City Agriculture and Forestry Institute fruit tree research institute, organic theatre, the Wei Shan village, Daxing District, Beijing and scientific research garden, school district, China Agricultural University west respectively; Mongolia's Rhizoma Cynanchi Stauntonii extract is provided by China Agricultural University biological control The Study on Resources center, can prepare by following method equally:
Cynanchum glaucescens plant under ground portion is cleaned, dried in the shade, place small-sized plant sample cracker to pulverize, after crossing 40 mesh sieves, at room temperature, the hydrochloric acid industrial alcohol lixiviate plant powder with 0.1% filters, filtrate decompression concentrate medicinal extract, filter residue continues the hydrochloric acid industrial alcohol lixiviate with 0.1%, so repeatedly, merges each time and gets the medicinal extract extract.
With total medicinal extract extract with 2% dissolve with hydrochloric acid solution, remove by filter insoluble matter, acid liquid is removed impurity with chloroform extraction, and sour water transfers pH to the alkaline solution of 9-10 with 10% sodium hydroxide mutually, use chloroform extraction 3-5 time more successively, concentrated chloroform mutually Mongolian Cynanchum glaucescens crude extract; Use the same method again and make with extra care for 2-3 time repeatedly, obtain the Cynanchum glaucescens essence extract.
1.2 Cynanchum glaucescens aqua Toxicity Determination technology
Get 1mg/mL Mongolia Cynanchum glaucescens essence extract aqua difference thin up 0.04mg/mL, 0.05mg/mL, 0.07mg/mL, 0.1mg/mL, 0.2mg/mL totally 5 concentration gradients, each concentration gradient arranges 4-5 repetition, in being placed with the culture dish of filter paper (R=4.5cm), evenly inject dilution 2mL respectively with the disposable needle tubing of 1mL, left standstill one hour, putting into 10 pear suckers then in each culture dish respectively, is contrast with distilled water.Put into raising in the incubator (RXZ, south of the River instrument plant) after connecing worm, 27 ℃ ± 1 ℃ of temperature, relative moisture is 70% ± 5%, photoperiod L:D=14:10, record death toll amount is calculated survival rate behind the 24h.
1.3 Rhizoma Cynanchi Stauntonii extract aqua field control pear sucker technology
At overwinter generation pear sucker occurance peak in spring, spray the Rhizoma Cynanchi Stauntonii extract aqua of 0.04mg/mL or 0.07mg/mL concentration with the special-purpose pesticide sprayer in orchard, when if the average pear sucker quantity of annotinous branch is lower than 3, spray the 0.04mg/mL aqua, if pear sucker density 3/more than the new branch, spray the 0.07mg/mL aqua, spray in succession respectively 2 times.0h, 24h, 48h, 96h, the 120h survival quantity of pear sucker adult on the branch of investigating behind the spray medicine is calculated the survival rate of adult.
Conventional dispenser consumption calculates: be example with 15 years age of tree pear trees, seeding row spacing is 2m * 4m, 4 pear tree area 8m
2, the special-purpose pesticide sprayer of orchard backpack (30L) spraying sprays 5 liters in Rhizoma Cynanchi Stauntonii extract aqua (pears leaf positive and negative sprays, be sprayed onto just having drips is as the criterion), and amount of dry matter is 0.04mg/mL*5000ml=0.2g, 0.07mg/ml*5000ml=0.35g, 1m
2The required Rhizoma Cynanchi Stauntonii extract amount of dry matter of fruit tree area is 0.0250g-0.0438g.
2 results and analysis
2.1 the Rhizoma Cynanchi Stauntonii extract aqua is to the indoor virulence of overwinter generation pear sucker adult
Discover that the Mongolian Rhizoma Cynanchi Stauntonii extract aqua of variable concentrations has significant lethal effect to overwinter generation pear sucker adult.After handling 24h, 0.07mg/mL Cynanchum glaucescens aqua reaches 100% to the lethal effect of overwinter generation pear sucker adult, and along with the reduction of concentration, preparation reduces the lethal effect of overwinter generation pear sucker adult, and the two correlation is (Fig. 1) obviously.The Cynanchum glaucescens aqua is 0.0375mg/mL to pear sucker adult 24h lethal concentration of 50 LC50 value.
2.2 the Rhizoma Cynanchi Stauntonii extract aqua is to the field control effect of pear sucker adult
Field trial finds that the Cynanchum glaucescens aqua has the pear sucker adult controls effect preferably, and behind the spray medicine 24h, the Cynanchum glaucescens aqua is 66.8% to pear sucker adult control efficiency, handles back 120h, and said preparation is 72.8%(Fig. 2 to the control effect of pear sucker adult).2.3 the Cynanchum glaucescens aqua is to the control effect of separate sources overwinter generation pear sucker adult
Desk research is found, the Mongolian Rhizoma Cynanchi Stauntonii extract aqua of variable concentrations has notable difference to the overwinter generation pear sucker lethal effect of different regions, Ma Lianwa theatre, Haidian (N39 ° of 57'52.84 "; the theatre, the Wei Shan village, pear sucker adult and Beijing Daxing of surviving the winter of E116 ° of 17'52.84 ") (N39 ° of 51'48.70 "; E116 ° of 29'51.20 ") the pear sucker adult that survives the winter is compared, the former is higher to the susceptibility of Cynanchum glaucescens aqua, under the middle low concentration to the lethal effect of pear sucker apparently higher than the latter (Fig. 3).
3 conclusions
Field and indoor pharmacodynamic test show that tentatively the Rhizoma Cynanchi Stauntonii extract aqua is obvious to the control action of overwinter generation pear sucker adult, namely show the significant control effect to the overwinter generation pear sucker behind the medication 24h.The pear sucker adult that survives the winter of finding different regions there are differences the susceptibility of Cynanchum glaucescens aqua, and this may be relevant to the resistance of medicament with the pear sucker adult that survives the winter, and needs further research.
This result of study is that further the research Rhizoma Cynanchi Stauntonii extract and is explored its corresponding mechanism of action and applied the Cynanchum glaucescens biologic product foundation is provided the mode of action of insect.
Claims (9)
1. the application of Rhizoma Cynanchi Stauntonii extract in the control pear sucker.
2. application according to claim 1 is killed pear sucker for Rhizoma Cynanchi Stauntonii extract is made water formulation.
3. application according to claim 1, described being applied as made the pear tree that the water formulation sprinkling is caused harm by pear sucker with Rhizoma Cynanchi Stauntonii extract.
4. application according to claim 3, the dosage of described sprinkling are 1m
2The required Rhizoma Cynanchi Stauntonii extract amount of dry matter of fruit tree area is 0.0250g-0.0438g.
5. application according to claim 1, described Rhizoma Cynanchi Stauntonii extract are the Alcoholic extraction thing of Cynanchum glaucescens.
6. application according to claim 1, described extract are medicinal extract extract, crude extract or essence extract.
7. application according to claim 6, described medicinal extract preparation method of extract is: Cynanchum glaucescens plant under ground portion is cleaned, dried in the shade, pulverize, at room temperature, the hydrochloric acid industrial alcohol lixiviate plant powder with 0.1% filters, filtrate decompression concentrate medicinal extract, filter residue continues to use the industrial alcohol lixiviate, so repeatedly, merges each time and gets the medicinal extract extract;
The preparation method of described crude extract is: with the medicinal extract extract with 2% dissolve with hydrochloric acid solution, remove by filter insoluble matter, acid liquid is removed impurity with chloroform extraction, and sour water is transferred pH9-10 with sodium hydroxide, use chloroform extraction 3-5 time more successively, concentrated chloroform mutually Mongolian Cynanchum glaucescens crude extract;
The preparation method of described essence extract is: with crude extract with 2% dissolve with hydrochloric acid solution, remove by filter insoluble matter, acid liquid is removed impurity with chloroform extraction, sour water is transferred pH9-10 with sodium hydroxide, use chloroform extraction 3-5 time more successively, concentrated chloroform mutually Mongolian Cynanchum glaucescens crude extract, 2-3 time repeatedly, obtain the Cynanchum glaucescens essence extract.
8. application according to claim 1, described pear sucker are overwinter generation pear sucker adult.
9. application according to claim 1, described Cynanchum glaucescens are Mongolian Cynanchum glaucescens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310153472.6A CN103190450B (en) | 2013-04-27 | 2013-04-27 | Application of cynanchum glaucescens extract in controlling psylla chinensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310153472.6A CN103190450B (en) | 2013-04-27 | 2013-04-27 | Application of cynanchum glaucescens extract in controlling psylla chinensis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103190450A true CN103190450A (en) | 2013-07-10 |
| CN103190450B CN103190450B (en) | 2014-04-16 |
Family
ID=48713417
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310153472.6A Expired - Fee Related CN103190450B (en) | 2013-04-27 | 2013-04-27 | Application of cynanchum glaucescens extract in controlling psylla chinensis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103190450B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010400A (en) * | 2015-07-06 | 2015-11-04 | 中国农业大学 | Method for trapping psylla chinensis nymphs |
| CN105660714A (en) * | 2014-11-20 | 2016-06-15 | 中国农业大学 | Pear psylla control medicament and application thereof in control of pear psylla |
| CN106034861A (en) * | 2016-06-01 | 2016-10-26 | 蚌埠市宝煦家庭农场 | Hebei pear tree pear psylla damage controlling method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156604A (en) * | 2007-11-12 | 2008-04-09 | 中国农业大学 | Cynanchum glaucescens extractive as well as preparing method and application |
-
2013
- 2013-04-27 CN CN201310153472.6A patent/CN103190450B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156604A (en) * | 2007-11-12 | 2008-04-09 | 中国农业大学 | Cynanchum glaucescens extractive as well as preparing method and application |
Non-Patent Citations (2)
| Title |
|---|
| 王洪平: "果树主要害虫测报方法（续）", 《北方果树》 * |
| 陈旭等: "白前提取物对斜纹夜蛾拒食活性的研究", 《中国生物防治》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660714A (en) * | 2014-11-20 | 2016-06-15 | 中国农业大学 | Pear psylla control medicament and application thereof in control of pear psylla |
| CN105660714B (en) * | 2014-11-20 | 2019-01-25 | 中国农业大学 | The medicament for preventing and treating pear sucker and its application in prevention and treatment pear sucker |
| CN105010400A (en) * | 2015-07-06 | 2015-11-04 | 中国农业大学 | Method for trapping psylla chinensis nymphs |
| CN105010400B (en) * | 2015-07-06 | 2018-04-10 | 中国农业大学 | A kind of method for trapping pear sucker nymph |
| CN106034861A (en) * | 2016-06-01 | 2016-10-26 | 蚌埠市宝煦家庭农场 | Hebei pear tree pear psylla damage controlling method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103190450B (en) | 2014-04-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Amadioha | Controlling rice blast in vitro and in vivo with extracts of Azadirachta indica | |
| CN100382707C (en) | A botanical pesticide | |
| CN103636660B (en) | A kind of for preventing and treating the Environmental-protection particle agent of Sugarcane Pests | |
| CN105284864A (en) | Forest fruit trunk-injection insecticide and preparation method and application thereof | |
| CN108041083B (en) | Plant source pesticide synergist and application thereof | |
| CN102283257B (en) | Spend the application of elephants-foot on control tobacco bacterial wilt in vain | |
| CN103190450B (en) | Application of cynanchum glaucescens extract in controlling psylla chinensis | |
| CN105613589A (en) | Herbicide as well as preparation method and use method thereof | |
| CN104886168A (en) | Biopesticide for preventing and treating tomato bacterial wilt and preparation method thereof | |
| CN104920487A (en) | Traditional Chinese medicine extract, preparation method thereof and method of utilizing same to prevent and control wheat aphid | |
| Idris et al. | Influence of argel (Solenostemma argel Del. Hayne) soil applications on flowering and yield of date palm (Phoenix dactylifera L.) | |
| CN103734117A (en) | Method for preventing empoasca vitis nymphs in organic ecological tea garden | |
| CN102487966B (en) | Botanical pesticide for effectively controlling Dioryctria splendidella Herrich-Schaeffer, preparation thereof and application thereof | |
| CN103004854A (en) | Allyl isothiocyanate and nicotine compound insecticide and application thereof | |
| CN112106789B (en) | Application of delphinium ethyl acetate extract in preventing and treating diamond back moth | |
| Sarra et al. | Histopathology of Culex pipiens (Linée, 1753)(Diptera, Culicidae) larvae exposed to the aqueous extract of Eucalyptus globulus l'Hér, 1789 (Myrtaceae) | |
| KR100976033B1 (en) | Use of plant extracts as antifungicides and insecticides | |
| Mekonnen et al. | Screening of botanical extracts for the control of Artemisia rehan (Artemisia absinthium L.) Aphid (Coloradoa absinthii Li.) in green house and field condition | |
| CN106490077A (en) | A kind of botanical pesticide for urban greening project and preparation method thereof | |
| CN103444794A (en) | Bactericide prepared from plant extract liquid | |
| CN102498984B (en) | Method for preventing and treating plutella xylostella by applying lemongrass essential oil | |
| CN102485002A (en) | Application of burdock extract in harmful citrus mite controlling | |
| CN105165902A (en) | Plant source herbicide and preparation method thereof | |
| CN101396024A (en) | Dendrolimus punctatus cytoplasmic polyhedrosis virus(dpcpv) bacillus thuringiensis wettable powder and preparation method thereof | |
| Khatun et al. | Insecticidal activity of Acacia catechu bark extract against four stored product pests |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140416 Termination date: 20160427 |