CN105010400B - A kind of method for trapping pear sucker nymph - Google Patents
A kind of method for trapping pear sucker nymph Download PDFInfo
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- CN105010400B CN105010400B CN201510390875.1A CN201510390875A CN105010400B CN 105010400 B CN105010400 B CN 105010400B CN 201510390875 A CN201510390875 A CN 201510390875A CN 105010400 B CN105010400 B CN 105010400B
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- nymph
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Abstract
The present invention relates to a kind of method for trapping pear sucker nymph.Present invention firstly discovers that and propose to trap a kind of method of its nymph using pear sucker honeydew.The result is shown：Pear sucker honeydew has obvious attractive effect to its nymph, and it traps degree and age without significant correlation, but can be limited by distance, and their rates of luring to nymph at 15cm and 25cm are respectively：It is 67.86% and 60.82% in 12h；It is 74.56% and 63.30% during 24h.So pear sucker nymph only has taxis to the honeydew on neighbouring blade, after more than certain distance, taxis is no longer obvious.Honeydew can mix administration with chemical pesticide in the future, play trapping effect, this measure, which can not only improve prevention effect, can also reduce the usage amount of chemical pesticide；This finds that the research for the aggregation of multiple of pear sucker nymph also in terms of certain provides theoretical foundation simultaneously.
Description
Technical field
The invention belongs to field of biological control, more particularly, to the secretion of pear sucker nymph honeydew to Psylla chinensis nymph
Attractive effect.
Background technology
Psylla Chinesis Yang Et is a kind of specially feeding habits insect, is distributed widely in each pears producing region in China, mainly with Pears and
Fruit is main harm object.Hazard approach mainly has two kinds：A kind of caused by piercing the juice of phyllidium, the spray tip and fruit
Directly harm or indirect hazard (transmitted virus)；Another kind is the honeydew of its secretion, a large amount of germs can be caused to grow, so as to cause
A large amount of mould dirty generations, and cause early leaf fall, shedding, influence tree vigo(u)r.It drastically influence the pears yield and quality in China.
At present to the prevention and control measure of pear sucker mainly based on chemical prevention, but the use of substantial amounts of chemical pesticide has been led
Cause the pear sucker resistance to the action of a drug, the problems such as a large amount of natural enemies are manslaughtered, residues of pesticides are exceeded occurs.In order to reduce these problems, it is badly in need of seeking
The use for looking for some green prevention and control technologies to replace or reduce chemical pesticide.Wherein pheromones have obtained in many pest controls
Accreditation and widely apply.The research of pear sucker pheromones is still in the elementary step, especially in terms of aggregation pheromone, although
If a large amount of reports prove pear sucker Eimeria Clustering pattern type, but its aggregation of multiple or specific influence factor are also not known (with reference to text
Offer：Zhao Jian, light intelligent beautiful, Zhao Shenghua, wait Spatial Distribution Pattern of Nymph of Psylla pyrisuga Forster Inner Mongol forest-science, and 2001, supplementary issue：
35-36. and Wang Liru, Xu Shaoqing, Xu Yongjiang, wait spatial distribution and the sampling techniques plant protection of Psylla Chinesis Yang Ets, and 2004,
30(1)：69-71.).Before this, research speculates that its aggregation habit may (bibliography relevant with spawning：The color quick pear suckers of king
Nymph Spatial distribution and sampling techniques research Henan Foresties science and technology, 2010,30 (4)：31-33.).Existing a large amount of report insects
Honeydew ant can be attracted come and supply its feeding, and ant can protect them from the attack (bibliography of natural enemy：Wang Si
The interaction and its Ecology Action applied entomology reports of inscription and old and clear ants with the Homoptera insect of excretion honeydew,
2011,48 (1)：183-190.).So, the honeydew of pear sucker is in addition to ant, if can also have aggtegation to similar, from
And the threat that common defence is external, it yet there are no report.
Because on the one hand aggtegation (Gao Changqi, Xu Guilian, Zhang Xiaojun, can wait should for carrying out insect dynamic monitoring
Monitored with aggregation pheromone with preventing and treating vertical pit cutting pin small moth Chinese forest disease pests, 2004,23 (2)：31-32.), on the other hand also
Concentration poisoning (bibliography can be carried out to insect as bait formulation：Jiang Yong, Lei Chaoliang, Zhang Zhongning insect aggregation pheromone elder brothers
Worm journal, 2002,45 (6)：822-832.).
The content of the invention
The present inventor has invented the method that nymph is trapped using the honeydew of pear sucker first, and is studied in detail, this
One invention will open up a new biological and ecological methods to prevent plant disease, pests, and erosion approach for the preventing and treating of China's pear sucker.
A kind of method for trapping pear sucker nymph, the thing that traps is pear sucker honeydew.
The thing that traps is positioned on Pears.
The thing that traps is from being no more than 25cm with a distance from pear sucker nymph.
The pear sucker honeydew is to be trapped pear sucker nymph itself secretion.I.e.：Pear sucker adult and/or nymph are put
With speckling with the Pears of medical cotton stick wiped clean of distilled water in test tube, it is placed in 25 DEG C of growth cabinets and raises afterwards
Support, being cut after 24 hours on blade has the part of a large amount of honeydews standby.
Low instar nymphs or the advanced age nymph in 4-5 age of the nymph for 1-3 ages.
Present invention firstly discovers that and proposition pear sucker honeydew attract and trap pear sucker nymph.
The result is shown：Pear sucker nymph has obvious taxis to honeydew, its taxis and age without significant correlation,
But it can be limited by distance, so pear sucker nymph only has taxis to the honeydew on neighbouring blade, when more than certain distance
Afterwards, taxis is no longer obvious.
Meaning of the present invention is：Honeydew can be applied in chemical prevention and control to the aggtegation of pear sucker nymph, can pole
The raising control effect of big degree simultaneously reduces the usage amount of chemical pesticide；Also pear sucker is carried out using the aggtegation of honeydew to lure
Catch preventing and treating.
Brief description of the drawings
Fig. 1 Psylla chinensis nymphs are illustrated to the selection test device in different taste sources；
The taxis test chart of Fig. 2 Psylla Chinesis Yang Et low instar nymphs；
The taxis test of Fig. 3 Psylla Chinesis Yang Et advanced age nymphs；
Fig. 4 different larval instars Psylla chinensis nymph contrasts to the taxis rate of honeydew；
Fig. 5 Psylla chinensis nymphs are tested the taxis of blade；
Response test schematic device of Fig. 6 Psylla chinensis nymphs to honeydew at different distance；
The influence that Psylla chinensis nymph is reacted honeydew taxis at Fig. 7 different distances.
Embodiment
The present invention is that experiment condition is completed indoors, tentatively specify that attractive effect of the pear sucker honeydew to its nymph,
Also provide a new thinking for the aggregation of multiple research of other similar insects simultaneously.The present invention is made in conjunction with embodiment into
The detailed description of one step.
Embodiment 1, Psylla chinensis nymph are to the selectivity in different taste sources
1 materials and methods
1.1 test material
Psylla chinensis nymph field acquisition method：The Pears for having Psylla chinensis nymph are adopted back in laboratory,
Raised.Collecting location is stabilized in Daxing district, Beijing, China in the Shahe village theatre of town, and main Pear Trees with Various Cultivars has gold, circle Huang Hefeng
Water.
Indoor feeding method：By the Psylla Chinesis Yang Et imago breeding that field collects on the high pears seedlings of 20-30cm, make it
Lay eggs and hatch above, so as to obtain Psylla chinensis nymph.
The acquisition of honeydew：After the Pears for having Psylla chinensis nymph are adopted back into laboratory, raise artificial in 25 DEG C
In climate box, the position for containing a large amount of honeydews on blade was cut with scissors in second day standby.
Mould dirty acquisition：The new blade adopted back will directly be cut standby with scissors containing mould dirty position.
Compare the acquisition of blade：Control is taken from handling the middle arteries position on same blade with blade, and is put into
With the medical cotton stick wiped clean for speckling with distilled water before test tube.
1.2 test method
Experiment is located at one and is covered with moistening filter paper, a diameter of 9cm culture dish, and processing is respectively that blade+honeydew is housed
In two vials of (mould dirt) and clean blade, and it is close to culture dish inwall and is placed in using culture dish center as symmetrical centre
Position, test worm (10/ware) is positioned over the center of culture dish, finally covered tightly culture dish with the preservative film of venting
(Fig. 1).Every 10 wares set 5 repetitions respectively as a repetition by low age (1-3 ages) and advanced age (4-5 ages).After 12 hours
The quantity of nymph in two test tubes is observed respectively.
1.3 computational methods
Result of the test is calculated it by following equation to the trend rate for handling and compareing, gained trend rate SPSS respectively
20.0 carry out T inspections or Duncan one-way analysis of variances.
Trend rate=Q/ (QT+QC) × 10
Chinese pear noise made in coughing or vomiting wood louse nymph quantity in Q=processing or contrast test tube；
Pear sucker nymph quantity in QT=processing test tubes；Pear sucker nymph quantity in QC=contrast test tubes
2. result and analysis
Psylla Chinesis Yang Et low instar nymphs show the Choice tests in different odor source：Psylla Chinesis Yang Et low instar nymphs select
Blade+mould dirty quantity and the quantity there was no significant difference (t=11.320 of control；Df=19；P=0.531>0.05)；Equally
Select the quantity also there was no significant difference (t=6.571 of blade and blank；Df=19；P=0.449>0.05)；But they are selected
Get rid of leaves piece+honeydew quantity be significantly higher than selection control quantity (t=5.295；Df=19；P=0.000<0.05) (Fig. 2).
Equally, Psylla Chinesis Yang Et advanced age nymph selection blade and blade+mould dirty quantity are compared with the control also without significantly
Sex differernce (t=-13.424；Df=19；P=0.136>0.05, t=-14.583；Df=19；P=0.260>0.05)；And select
Get rid of leaves piece+honeydew quantity be significantly more than select control quantity (t=-6.164；Df=19；P=0.000<0.05) (Fig. 3).
Result is shown simultaneously：Although Psylla Chinesis Yang Et advanced age nymph is slightly below low to the taxis (75.82%) of blade+honeydew
Age nymph (84.73%), but there is no significant difference (F=3.490 between them；Df=1,38；P=0.069>0.05) (figure
4)。
And contrast selection space management and quantity that only blade is handled, it can be seen that there was no significant difference between them (t=
8.361；Df=19；P=0.302>0.05, t=8.104；Df=19；P=0.662>0.05, t=8.699；Df=19；P=
0.861>0.05), illustrate that they do not have obvious taxis to blade, so as to eliminate influence (Fig. 5) of the blade to nymph.
Embodiment 2, Psylla chinensis nymph are evaluated the taxis of honeydew
1 materials and methods
1.1 test material
Field collection, raising and the acquisition method of honeydew and blade of Psylla chinensis nymph are the same as example one.
1.2 test method
Experimental rig connects two long teat glass (15cm) by a hard chart drum and form, wherein blade+honeydew with
Clean blade is respectively placed in the bottom of two test tubes, and the centre bit of device puts test worm (10/device), and measuring distance passes through
Pull two test tubes control test worm and the distance of honeydew (Fig. 6).Measuring distance is 15cm and 25cm, and observing time is respectively
After 12h and 24h, observed content is the quantity in two test tube bottom nymphs.Every 10 devices set 5 altogether as a repetition
Individual repetition.
1.3 computational methods
Computational methods of the interpretation of result with example one.
2 results and analysis
Taxis of the Psylla chinensis nymph to honeydew is specify that, further experiment is found：Whether 12h or 24h, China
Pear sucker nymph is significantly greater than the (F=11.410 at 25cm to the taxis of honeydew at 15cm；Df=1,4；P=0.028<
0.05, F=9.081；Df=1,4；P=0.039<0.05).But when distance between the two reach 25cm it is remote when, pear sucker
The extension no longer over time of the taxis of nymph and dramatically increase (F=2.217；Df=1,4；P=0.211>0.05) (Fig. 7).
It can thus be appreciated that response distance of the Psylla chinensis nymph to honeydew is limited, i.e., honeydew only has to the nymph on neighbouring blade
Attractive effect, but after certain distance is exceeded, effect of the honeydew to pear sucker nymph is gradually reduced, this result is that field exists
Pear sucker nymph is trapped using honeydew provide reference in coverage.
Claims (3)
1. a kind of method for trapping pear sucker nymph, the thing that traps is pear sucker honeydew, and the pear sucker honeydew is to be trapped
Itself secretion of pear sucker nymph, the thing that traps is from being no more than 25cm with a distance from pear sucker nymph.
2. according to the method for claim 1, the thing that traps is positioned on Pears.
3. according to the method for claim 1, low instar nymphs or the advanced age nymph in 4-5 age of the nymph for 1-3 ages.
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| CN110896927A (en) * | 2019-11-29 | 2020-03-24 | 中国农业大学 | Indoor artificial propagation method for psylla chinensis |
| CN116098136A (en) * | 2023-03-09 | 2023-05-12 | 河北省农林科学院植物保护研究所 | Method for attracting pear psyllids in pear garden by combining different-color insect attracting plates |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648703A (en) * | 2011-02-28 | 2012-08-29 | 汪鹏丞 | Artificial aphid breeding and honeydew preparation technique |
| CN103190450A (en) * | 2013-04-27 | 2013-07-10 | 中国农业大学 | Application of cynanchum glaucescens extract in controlling psylla chinensis |
| CN104222078A (en) * | 2014-09-03 | 2014-12-24 | 华南农业大学 | Composition for trapping diaphorina citri kuwayama and application method of composition |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648703A (en) * | 2011-02-28 | 2012-08-29 | 汪鹏丞 | Artificial aphid breeding and honeydew preparation technique |
| CN103190450A (en) * | 2013-04-27 | 2013-07-10 | 中国农业大学 | Application of cynanchum glaucescens extract in controlling psylla chinensis |
| CN104222078A (en) * | 2014-09-03 | 2014-12-24 | 华南农业大学 | Composition for trapping diaphorina citri kuwayama and application method of composition |
Non-Patent Citations (1)
| Title |
|---|
| "Sex-specific responses of Asian citrus psyllid to volatiles of conspecific and host-plant origin";A. Moghbeli Gharaei.etal;《J. Appl. Entomol》;20141231(第138期);摘要 * |
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