CN103184210B - 一种定点突变改造的基因工程腈水解酶 - Google Patents
一种定点突变改造的基因工程腈水解酶 Download PDFInfo
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- CN103184210B CN103184210B CN201310069190.8A CN201310069190A CN103184210B CN 103184210 B CN103184210 B CN 103184210B CN 201310069190 A CN201310069190 A CN 201310069190A CN 103184210 B CN103184210 B CN 103184210B
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- nitrilase
- genetically engineered
- aminoacid sequence
- seqidno
- fungal
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Pfu enzyme | 0.5 μL |
10×buffer | 5 μL |
Template DNA | 1 μL |
dd H2O | 38.5 μL |
dNTP | 3 μL |
Forward primer | 1 μL |
Reverse primer | 1 μL |
Total | 50 μL |
DpnI | 1 μL |
10×buffer | 2 μL |
DNA | 10 μL |
dd H2O | 7 μL |
Total | 20 μL |
Claims (9)
Priority Applications (1)
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CN201310069190.8A CN103184210B (zh) | 2013-03-05 | 2013-03-05 | 一种定点突变改造的基因工程腈水解酶 |
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CN201310069190.8A CN103184210B (zh) | 2013-03-05 | 2013-03-05 | 一种定点突变改造的基因工程腈水解酶 |
Publications (2)
Publication Number | Publication Date |
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CN103184210A CN103184210A (zh) | 2013-07-03 |
CN103184210B true CN103184210B (zh) | 2016-04-06 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103667228B (zh) * | 2013-12-14 | 2016-08-17 | 江南大学 | 一种催化活力及热稳定性提高的真菌腈水解酶突变体及其构建方法 |
CN104212784B (zh) * | 2014-08-12 | 2017-06-23 | 浙江工业大学 | 重组腈水解酶、编码基因、突变体、工程菌及应用 |
CN108486088B (zh) * | 2018-02-14 | 2021-02-02 | 浙江工业大学 | 腈水解酶突变体及其应用 |
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2013
- 2013-03-05 CN CN201310069190.8A patent/CN103184210B/zh active Active
Non-Patent Citations (2)
Title |
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Fungal His-Tagged Nitrilase from Gibberella intermedia:Gene Cloning,Heterologous Expression and Biochemical Properties;Jin-Song Gong,et al;《PLOS ONE》;20121130;第7卷(第11期);e50622,第1页摘要,第2页左栏第3段、右栏第2-3段,第3页图2,第4页第3段 * |
Identification of Amino Acide Residues Responsible for the enantioselectivity and Amide Formation Capacity of the Arylacetonitrilase from Pseudomonas fluorescens EBC191;Christoph Kiziak,et al;《Applied and Environmental Microbiology》;20090930;第75卷(第17期);第5592页摘要,第5593-5597页结果 * |
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CN103184210A (zh) | 2013-07-03 |
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Inventor after: Gong Jinsong Inventor after: Xiong Lei Inventor after: Xu Zhenghong Inventor after: Shi Jinsong Inventor after: Li Heng Inventor after: Sun Wenjing Inventor after: Zhou Qiang Inventor after: Rong Qinghong Inventor before: Gong Jinsong Inventor before: Xiong Lei Inventor before: Xu Zhenghong Inventor before: Shi Jinsong Inventor before: Li Heng Inventor before: Sun Wenjing Inventor before: Zhou Qiang |
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Effective date of registration: 20160120 Address after: No. 1800 road 214122 Jiangsu Lihu Binhu District City of Wuxi Province Applicant after: Jiangnan University Applicant after: Jiangxi Dexing PARCHN Sodium VC Co., Ltd. Applicant after: Gunther bio tech ltd, Shandong Address before: No. 1800 road 214122 Jiangsu Lihu Binhu District City of Wuxi Province Applicant before: Jiangnan University Applicant before: Jiangxi Dexing PARCHN Sodium VC Co., Ltd. |
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