CN103141311A - Method for reducing burley tobacco nicotine - Google Patents
Method for reducing burley tobacco nicotine Download PDFInfo
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- CN103141311A CN103141311A CN2013100613951A CN201310061395A CN103141311A CN 103141311 A CN103141311 A CN 103141311A CN 2013100613951 A CN2013100613951 A CN 2013100613951A CN 201310061395 A CN201310061395 A CN 201310061395A CN 103141311 A CN103141311 A CN 103141311A
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- Prior art keywords
- grafting
- burley tobacco
- nicotine
- stock
- tomato
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- 241000208125 Nicotiana Species 0.000 title claims abstract description 53
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 52
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 title claims abstract description 33
- 229960002715 nicotine Drugs 0.000 title claims abstract description 33
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 14
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims abstract description 21
- 240000003768 Solanum lycopersicum Species 0.000 claims description 17
- 235000019504 cigarettes Nutrition 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 claims description 9
- 230000035876 healing Effects 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 2
- 238000013022 venting Methods 0.000 claims description 2
- 241000208292 Solanaceae Species 0.000 abstract description 2
- 239000002689 soil Substances 0.000 abstract description 2
- 241000227653 Lycopersicon Species 0.000 abstract 4
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000004581 coalescence Methods 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 239000000796 flavoring agent Substances 0.000 description 7
- 235000019634 flavors Nutrition 0.000 description 7
- 239000003205 fragrance Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 238000001035 drying Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 241001002356 Valeriana edulis Species 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000195940 Bryophyta Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
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- Cigarettes, Filters, And Manufacturing Of Filters (AREA)
Abstract
The invention discloses a method for reducing burley tobacco nicotine, which comprises the following steps: selecting a tomato seedling as a stock and a burley tobacco seedling as a cion, grafting by adopting a cleft graft method, horizontally cutting off the upper half part of the stock that is 8 cm to 10 cm from the soil surface, and vertically downwards cutting a cut with the length of 1 cm; cutting off the upper half part of the cion from a parent, grinding two sides of a stem into wedge-shaped structures with the length of 1 cm, vertically inserting the wedge-shaped structure into the stock, fixing by a grafting clip, and moving into a coalescence curing chamber. The synthesis of the burley tobacco rooted nicotine is controlled manually through a grafting manner, the burley tobacco nicotine is reduced remarkably, and the problem that the content of the burley tobacco nicotine is too high is solved effectively. As burley tobacco and tomatoes are classified as solanaceae, the burley tobacco and the tomato have good affinities, and the grafting success rate reaches more than 70%. The content of nicotine of the burley tobacco and tomato combination is reduced remarkably, the field growth condition is basically consistent with the cion own-rooted seedling, and the acre yield remains almost the same.
Description
Technical field
The present invention relates to burley tobaccos culture technique field, refer to particularly a kind of method that reduces burley tobaccos nicotine.
Background technology
Burley tobaccos are primary raw materials of making blended type cigarette, have the features such as blade is thin and light, institutional framework is loosened and not coarse, and elasticity is strong, and the filling value is high, and the absorption fragrance performance is good, are bringing into play unique effect in cigarette composition.At present, domestic burley tobaccos nicotine content of tobacco leaves is too high, has had a strong impact on the use of burley tobaccos in cigarette composition, and its availability and resource utilization all reduce, and nicotine content is too high is also the main factor that affects its use value.For the too high difficult problem of burley tobaccos nicotine content, domestic research is more, and result shows: can reduce to a certain extent burley tobaccos nicotine by reducing amount of nitrogen per mu, but the also corresponding reduction of its per mu yield, the per mu yield benefit reduces; Stay the number of sheets to reduce burley tobaccos nicotine effect by increase not remarkable, and easily reduce burley tobaccos tobacco leaf interior quality, the quality of especially smokeing panel test reduces obviously.Therefore, by reducing amount of nitrogen per mu, increasing the method that measures such as staying the number of sheets reduces burley tobaccos nicotine and be difficult to solve preferably the too high difficult problem of burley tobaccos nicotine.Method provided by the present invention is a kind ofly can significantly reduce nicotine content, guarantees its per mu yield, and has improved the method that tobacco leaf is inhaled flavor.
Summary of the invention
The purpose of this invention is to provide a kind of method that reduces burley tobaccos nicotine, synthetic by grafting method manual control nicotine, thus reduce the burley tobaccos nicotine content.
Technical scheme of the present invention: a kind of method that reduces burley tobaccos nicotine comprises the following steps:
(1) choose plant height 12cm-14cm, the tomato seedling of stem girth 0.5cm-0.6cm is stock; More than choosing plant height 4.0 cm, the burley tobaccos cigarette seedling of 5 leaf 1 hearts is scion;
(2) cut away the first half at stock apart from native face 8cm-10cm level, then cut vertically downward the otch of long 1cm; Scion the first half is downcut from parent, the two sides of stem is whittled into wedge shape, long 1cm vertically inserts stock, fixes with Grafting clip, moves into the healing fog room;
(3) front 3 days lucifuges after grafting, sealing is controlled air humidity more than 90%, 25 ~ 28 ℃ of temperature, 3 days rear ventings and see light are controlled air humidity and are not wilted as benchmark take scion, to healing fully, shift out fog room after grafting healing fully after 10 days to 12 days.
Carry out in the environment that grafting should be suitable in temperature, light is weak, cause the grafting wilting to avoid strong illumination.Grafting tool need disinfect in alcohol, and grafting personnel's hand need clean up.
The invention has the advantages that: the present invention artificially promotes the material of burley tobaccos and two kinds of different plant species of tomato to carry out natural fusion by grafting method, utilize Tomato Root System displacement burley tobaccos root system, synthesizing of manual control burley tobaccos root system nicotine, obviously reduce burley tobaccos nicotine, efficiently solved the too high problem of burley tobaccos nicotine content.Because burley tobaccos and tomato all belong to Solanaceae, have affinity preferably, its graft survival rate reaches more than 70%.The nicotine content of burley tobaccos/Tomato Grafting combination obviously reduces, and its field growing way and scion own-rooted tree are basically identical, and per mu yield remains basically stable.The quality of smokeing panel test shows that also the peculiar aromatic odour of grafting combination burley tobaccos decreases, but excitant obviously reduces, strength reduces, especially tabacco fragrance has the uniqueness suction flavor feature like tomato flavor, industrial applicability obviously strengthens, and can be applied in the Virginian-type cigarette formula, widen the burley tobaccos scope of application, improve resource utilization.
Embodiment:
The present invention is described in further detail below in conjunction with specific embodiment.
The embodiment test is carried out in Swan Lake tobacco leaf test garden, Wuhan City, Hubei Province, and concrete operation step is as follows:
(1) seedling medium: be the 2:1 preparation by the peat composed of rotten mosses and perlite volume ratio, every m
3Matrix adds 50% carbendazol wettable powder 200g, and nitrogen, phosphorus, potassium content are 15:15:15 Nitrogen, Phosphorus and Potassium 3kg, and fertile medicine mixes.
(2) scion and stock are cultivated: tomato variety is No. 8, Chongqing powder, and the burley tobaccos kind is E'yan 1.Carry out in steelframe temperature canopy, burley tobaccos sowing time adopts lukewarm water that tomato seeds is soaked seed than early 15 days sowing time of tomato, and the sowing again that shows money or valuables one carries unintentionally after vernalization is to control the growth potential of two seedlings, standby grafting.No. 8 quantity of tomato seedling Chongqing powder are about 500 strains, and E'yan 1 cigarette seedling quantity is about 1000 strains.
(3) scion and stock are selected: choose plant height 12cm-14cm, the tomato seedling of stem girth 0.5cm-0.6cm is stock; More than choosing plant height 4.0 cm, the burley tobaccos cigarette seedling of 5 leaf 1 hearts is scion.
(4) grafting tool: blade, use double-edged razor blade, it vertically is converted into 2 gets final product, in time change during edges dull.Grafting clip: fit tightly in order to make stock and scion tangent plane, fix with the plastics Grafting clip in grafting part.
(5) grafting: adopt the cleft graft grafting.Cut away the first half tomato apart from native face 8cm-10cm level, then cut vertically downward the otch of long 1cm; Burley tobaccos scion the first half is downcut from parent, stem is whittled into wedge shape, long 1cm vertically inserts stock, fixes with Grafting clip, moves into rapidly the chamber of healing.
(6) managing after grafting: loseed light in front 3 days after grafting, stuffy, air humidity is more than 90%, temperature 25-28 ℃.After 3 days, increase gradually the time of ventilating and seeing light, reduce air humidity, to healing fully.Shift out fog room after 12 days.
(7) grafting points for attention: carry out in the environment that grafting should be suitable in temperature, light is weak, cause the grafting wilting to avoid strong illumination.Grafting tool need disinfect in alcohol, and grafting personnel's hand need clean up.
(8) transplant: embodiment grafting combination and E'yan 1 own-rooted tree are transplanted to the land for growing field crops, and seeding row spacing is 110cm * 55cm, and 321 strains are transplanted in embodiment grafting combination, and the E'yan 1 own-rooted tree is transplanted 500 strains.
(9) transplant after field management and the drying in the air system of gathering: according to the management of E'yan 1 variety culture and gather and dry in the air measure requirement processed to embodiment grafting combination and the E'yan 1 own-rooted tree is transplanted rear field management and the system of drying in the air of gathering.
Result of the test is as follows:
(1) the embodiment grafting is combined into the motility rate result
The grafting combination that No. 8, experimental example burley tobaccos E'yan 1 and tomato Chongqing powder amounts to 450 strains, removes fog room after 12 days, when transplanting to the land for growing field crops, finally survives 321 strains, and survival rate is 71.3%.
(2) embodiment grafting combination economical character result
The plant height of experimental example grafting combination and burley tobaccos own-rooted tree, effectively stay the number of sheets and the leaf economical character no significant difference such as wide that comes into leaves.From the field actual conditions, embodiment grafting combination and burley tobaccos own-rooted tree growing way appearance are basically identical, no significant difference.
Table 1 economical character contrast table (after pinching)
(3) embodiment grafting combination per mu yield result
Gross yield such as the table 1 of embodiment grafting combination, after planting cigarette 1100 strain conversions according to mu, embodiment grafting combination per mu yield is 168.1Kg/ mu, E'yan 1 own-rooted tree per mu yield is 167.5Kg.Result of the test shows: embodiment grafting combination and contrast per mu yield no significant difference can guarantee its output.
Table 2 per mu yield is contrast table as a result
(4) embodiment grafting combination nicotine content result
The nicotine content comparing result of embodiment grafting combination shows: the grafting combination with compare, the nicotine of upper, middle and lower position tobacco leaf obviously reduces, the reduction amplitude reaches more than 72%, is up to 82.2%.Synthetic in theory by tobacco root from nicotine, after the present embodiment grafting combination is replaced as Tomato Root System by tobacco root, its nicotine content should be 0, but in the reality cultivation, because the grafting callus place of grafting combination also is coated with soil, a lot of lateral roots have been sent out at the tobacco stem place, and this may be also to cause grafting to make up the reason that tobacco leaf contains nicotine, but this does not affect the result of the test that the grafting combination can reduce nicotine content.
Table 3 nicotine content result contrast (%)
(5) embodiment grafting combination smoking result
Embodiment tests plant and gathers after the system of drying in the air, and gets the middle part tobacco sample and compares and smoke panel test, and smokes panel test by the cigarette composition expert appraisal.The embodiment result shows: the peculiar aromatic odour of burley tobaccos of embodiment grafting combination weakens to some extent, but excitant and strength reduce, especially tabacco fragrance has the uniqueness suction flavor feature like tomato flavor, industrial applicability strengthens, may be used in the Virginian-type cigarette formula, widen the burley tobaccos scope of application, improved resource utilization.
The quality contrast table of smokeing panel test is made up in table 2 grafting
Overall merit: comparatively speaking, the perfume quantity of grafting combination is slightly owed, and the peculiar fragrant breath of burley tobaccos weakens to some extent, but excitant and strength are all less, especially tabacco fragrance has the uniqueness suction flavor feature like tomato flavor, and industrial applicability obviously strengthens, and may be used on the Virginian-type cigarette formula.
Claims (1)
1. method that reduces burley tobaccos nicotine comprises the following steps:
(1) choose plant height 12cm-14cm, the tomato seedling of stem girth 0.5cm-0.6cm is stock; More than choosing plant height 4.0 cm, the burley tobaccos cigarette seedling of 5 leaf 1 hearts is scion;
(2) cut away the first half at stock apart from native face 8cm-10cm level, then cut vertically downward the otch of long 1cm; Scion the first half is downcut from parent, the two sides of stem is whittled into wedge shape, long 1cm vertically inserts stock, fixes with Grafting clip, moves into the healing fog room;
(3) front 3 days lucifuges after grafting, sealing is controlled air humidity more than 90%, 25 ~ 28 ℃ of temperature, 3 days rear ventings and see light are controlled air humidity and are not wilted as benchmark take scion, to healing fully, shift out fog room after grafting healing fully after 10 days to 12 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100613951A CN103141311A (en) | 2013-02-27 | 2013-02-27 | Method for reducing burley tobacco nicotine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100613951A CN103141311A (en) | 2013-02-27 | 2013-02-27 | Method for reducing burley tobacco nicotine |
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| Publication Number | Publication Date |
|---|---|
| CN103141311A true CN103141311A (en) | 2013-06-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100613951A Pending CN103141311A (en) | 2013-02-27 | 2013-02-27 | Method for reducing burley tobacco nicotine |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532269A (en) * | 2015-12-31 | 2016-05-04 | 湖南农业大学 | Method for adjusting and controlling nicotine content of tobacco |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101133705A (en) * | 2007-09-27 | 2008-03-05 | 王旭强 | Serpent melon grafting method |
| CN102487731A (en) * | 2011-11-29 | 2012-06-13 | 红云红河烟草(集团)有限责任公司 | Method for improving aromatic tobacco SAM variety |
-
2013
- 2013-02-27 CN CN2013100613951A patent/CN103141311A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101133705A (en) * | 2007-09-27 | 2008-03-05 | 王旭强 | Serpent melon grafting method |
| CN102487731A (en) * | 2011-11-29 | 2012-06-13 | 红云红河烟草(集团)有限责任公司 | Method for improving aromatic tobacco SAM variety |
Non-Patent Citations (3)
| Title |
|---|
| JUAN M等: "nicotine-free and salt-tolerant tobacco plants obtained by grafting to salinity-resistant rootstocks of tomato", 《PHYSIOLOGIA PLANTARUM》 * |
| 戴冕等: "烟草马铃薯嫁接体中的烟碱积累", 《植物生理学报》 * |
| 时向东等: "稳定氮同位素示踪技术在烟草研究中的应用", 《中国烟草学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532269A (en) * | 2015-12-31 | 2016-05-04 | 湖南农业大学 | Method for adjusting and controlling nicotine content of tobacco |
| CN105532269B (en) * | 2015-12-31 | 2018-06-01 | 湖南农业大学 | A kind of method of regulation and control Nicotine Content in Tobacco Leaf |
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Application publication date: 20130612 |