CN105532269A - Method for adjusting and controlling nicotine content of tobacco - Google Patents
Method for adjusting and controlling nicotine content of tobacco Download PDFInfo
- Publication number
- CN105532269A CN105532269A CN201511030523.1A CN201511030523A CN105532269A CN 105532269 A CN105532269 A CN 105532269A CN 201511030523 A CN201511030523 A CN 201511030523A CN 105532269 A CN105532269 A CN 105532269A
- Authority
- CN
- China
- Prior art keywords
- tobacco
- grafting
- nicotine content
- eggplant
- ridging
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Manufacture Of Tobacco Products (AREA)
Abstract
The invention relates to the field of tobacco planting, and particularly discloses a method for adjusting and controlling nicotine content of tobacco. The nicotine content of the tobacco is adjusted and controlled by combining grafting with the means of adjusting and controlling transplanting time and the ridging height. The overground part of the tobacco is grafted to a Solanum torvum rootstock with no nicotine synthesis ability, tobacco plants with reconstructed root systems are obtained, the occurrence amount of adventitious roots is artificially controlled according to different ridging heights, and the purpose of adjusting and controlling the nicotine synthesis amount and flue-cured tobacco nicotine content is achieved by controlling nicotine synthesis sources (root systems). The grafting combination affinity is good, the grafting survival rate is high and reaches 95% or above, the grafted tobacco plants normally grow and have no remarkable difference compared with own-rooted tobacco plants, nutrient absorption is normal, and other quality indexes of flue-cured tobacco leaves are reserved while low nicotine content is achieved.
Description
Technical field
The present invention relates to tobacco planting field, specifically, relate to a kind of regulate and control the method for Nicotine Content in Tobacco Leaf.
Background technology
Flue-cured tobacco is the primary raw material of Chinese-style cigarette.The object of people's smoking sucks nicotine, thus obtain physiological satisfied, and nicotine single index shared weight in Chemical Compositions of Flue-cured Tobacco assessment indicator system reaches 0.17, and nicotine is the key factor determining Flue-cured tobacco Quality.At present, the problem that Flue-cured Tobacco alkali content is higher, seriously governs the industrial applicability of China's flue-cured tobacco.For this problem, domestic scholars has carried out large quantifier elimination.As, reduce nitrogen fertilizer amount and can reduce Flue-cured Tobacco alkali content to a certain extent, but can yield of tobacco be reduced, and can have a negative impact to the perfume quantity of tobacco leaf; Adjustment Topping Time and increase leaves remained, also can regulate and control Flue-cured Tobacco alkali content, but the former Topping Time is not easily held, increasing leaves remained then can have harmful effect to other index of quality of flue-cured tobacco, and such as middle and upper part leaf hacks and tobacco sensory quality; Also have the report by using plant growth regulator regulation and control nicotine content, but result also in plant growth regulator remaining in tobacco leaf, plant growth regulator consumption is less in addition, and consumption slightly loses use value with quality of tobacco accidentally then can be caused to become bad; In addition, also have by girdling, cut the report that the field measures such as root reduce nicotine content, but workload is comparatively large, and field operation easier is high, does not thus put it over.Domestic and international research confirms, nicotine in tobacco synthesizes in root, the present invention is based on this basic theory, adopt the way that grafting and ridging technology combine, reconstruct nicotine synthetic source---flue-cured tobacco root system, and then have developed a kind of nicotine content quantitatively regulating and controlling technology, not only can quantitatively regulating and controlling Flue-cured Tobacco alkali content and yield of tobacco and quality can be ensured to a certain extent.Through retrieval, the report of technology of the quantitatively regulating and controlling Flue-cured Tobacco alkali content that do not find to combine with grafting and ridging technology.
Summary of the invention
In order to solve problems of the prior art, the object of this invention is to provide a kind of regulate and control the method for Nicotine Content in Tobacco Leaf.
In order to realize the object of the invention, technical scheme of the present invention is as follows:
The invention provides a kind of regulate and control the method for Nicotine Content in Tobacco Leaf, cross and grafting combined with regulation and control transplanting time and means highly of earth up, regulate and control the nicotine content of tobacco.
Further, described method comprises the steps:
S1, utilize eggplant as stock, carry out the scion grafting of tobacco;
S2, grafting scion and stock heal completely, during newborn blade 1-2 sheet, by the transplantation of seedlings of grafting cigarette to land for growing field crops, and carry out plastic mulching, keep grafting mouth more than ground;
When S3, grafting plant height reach 70-80cm, carry out the ridging of differing heights, ridging height is not to cover grafting mouth or to cover grafting mouth 2cm ~ 10cm for degree.
Described ridging height can adjust according to the demand of tobacco leaf to nicotine content, to meet target needs.
When obtaining nicotine content in advance and being the tobacco leaf of 0.002% ~ 0.005%, ridging is highly that gained tobacco leaf can be used as the inserts without nicotine or micro-nicotine not cover grafting mouth for degree.
When obtaining nicotine content in advance and being the tobacco leaf of 1.1% ~ 1.3%, ridging height is to cover grafting mouth 4 ~ 6cm for degree, and gained tobacco leaf can be used as the inserts of low nicotine.
When obtaining nicotine content in advance and being the tobacco leaf of 1.4% ~ 1.6%, ridging height is to cover grafting mouth 6 ~ 8cm for degree, and gained tobacco leaf can be used as the main material cigarette of having reduced nicotine content.
When obtaining nicotine content in advance and being the tobacco leaf of 1.9% ~ 2.1%, ridging height is to cover grafting mouth 8 ~ 10cm for degree, and gained tobacco leaf can be used as the main material cigarette of medium grade cigarette alkali content.
Further, in order to better realize the scion grafting of tobacco, described S1 comprises the steps:
(1) nursery: by eggplant seed and tobacco seed seeding and seedling raising;
(2) grafting: eggplant rootstock root is retained together with a part of stem stalk, and guarantee to leave 2 blades, remainder excises, by dark to incision 1 ~ 2cm for the stem top pars intermedia of eggplant rootstock; Scion root is excised, retains scion 4 leaf 1 heart, stem is whittled into long wedge, the long 1.5cm ~ 1.8cm of bevel; Tobacco scion is inserted the otch of eggplant rootstock, flue-cured tobacco scion and Solanum Torvum stock intimate surface are fitted, fixes with Grafting clip;
(3) managing after grafting: first 3 days after grafting, the shade net whole day of sheltering from heat or light rate with 85% was sheltered from heat or light, and at noon 10 ~ 14 time spraying to keep air humidity; Within 4th day, rise, before morning 9, after when afternoon 14, can shade net be opened, and between increasing gradually when ventilating and see light; Field run plant period management is proceeded to after 12 days.
Described eggplant seed can be any, and commercially available eggplant seed all can realize technical solution of the present invention.In order to ensure grafting and the growth quality of tobacco, in the specific embodiment of the present invention, select wild species Solanum Torvum seed as eggplant kind.
More specifically, described step (1), by eggplant seed and tobacco seed seeding and seedling raising, is grown to 7 ~ 8 true leaves until eggplant seedling, tobacco seedling is when growing to 5 leaf 1 heart, eggplant seedling and tobacco seedling are taken out and prepare grafting.
Beneficial effect of the present invention is:
The present invention by the acrial part grafting of tobacco on the Solanum Torvum stock not possessing synthesis nicotine ability, obtain the cigarette strain after root system reconstruct, after field-transplanting, again by the generating capacity of different ridgings height manual control adventive root, by controlling the method for nicotine synthetic source (root system), realize the object of regulation and control nicotine synthetic amount and Flue-cured Tobacco alkali content; Grafting combination affinity of the present invention is better, and graft survival rate is higher, reaches more than 95%; Grafting cigarette strain growing way is normal, compares without significant difference with the strain of own-rooted tree cigarette, and Nutrient Absorption is normal, and cured tobacco leaf is while realizing having reduced nicotine content, and other index of quality are retained.
Embodiment
Below in conjunction with embodiment, the preferred embodiment of the present invention is described in detail.It will be appreciated that providing of following examples is only object in order to play explanation, being not used to limit scope of the present invention.Those skilled in the art, when not deviating from aim of the present invention and spirit, can carry out various amendment and replacement to the present invention.
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
The acquisition of embodiment 1 grafting
(1) stock is cultivated
March 9, be that the Gibberellins solution of 100mg/L is soaked after 48h by Solanum Torvum seed concentration, clean, and put into climate box March 11 and carry out change temperature-accelerated germination (daytime 25 DEG C, 12h; Night 16 DEG C of 12h), March 17,50% seed showed money or valuables one carries unintentionally, and adopted tray seedling growth method to be sown into (Friendly tobacco seedling raising medium) in matrix, 1, every cave.After broadcasting, seedling-cultivating tray is placed in green house, and strengthens water and fertilizer management.
(2) scion cultivated
March 23, when the rootstock seedling two panels cotyledon of more than 50% launches, carry out flue-cured tobacco seedling-cultivating, kind is K326 (capsuled seed), and adopt floating seedlings method, matrix selects commercially available Friendly tobacco seedling raising medium.After planting seedling-cultivating tray is placed in green house, and strengthens water and fertilizer management.
(3) grafting
May 2, choose the Solanum Torvum seedling of plant height about 15cm, thick more than the 0.5cm of stem, the blade excision stock top of sterilizing in the position apart from matrix 10cm, retains 2 leaves, Vertical dimension incision 1.5cm; Choose the K326 cigarette seedling of 5 leaves wholeheartedly growing way value, excision root, basal part of stem is whittled into wedge, vertical insertion stock otch, guarantee that scion bevel and stock tangent plane fit tightly, fix with Grafting clip, grafting puts into the Small plastic shed being stamped the curtain that shelters from heat or light, shelter from heat or light with the sunshade net of the rate of sheltering from heat or light 85% after grafting, throw off shade net gradually depending on wilting degree.Strengthen water and fertilizer management, note watering employing infusion method, avoid grafting Wound contact water, May 13, stock scion healing cigarette seedling grafting success, reaches 98% through statistics graft survival rate.
The field trial of embodiment 2 grafting
May 20, carry out the transplanting of flue-cured tobacco grafting, grafting and K326 own-rooted tree are transplanted to land for growing field crops, seeding row spacing 0.5m*1.2m, and make grafting mouth at above about the 2cm in face, ridge.Grafting is transplanted to land for growing field crops, notes during transplanting grafting wound not being planted burying, transplanting when within latter 30 days, to take off film ridging, by arranging different ridging height, the inducing amount of manual control adventive root, and then reaching the object controlling nicotine synthetic amount.
4 process are established in test, and concrete processing method is as follows:
Table 1 is earthed up and is highly processed full edition
| process | processing method |
| process 1 | grafting plant height reaches 70-80cm and takes off film ridging, and ridging height is not to cover grafting mouth for degree. |
| process 2 | grafting plant height reaches 70-80cm and takes off film ridging, and ridging height is to cover grafting mouth 4-6cm for degree. |
| process 3 | grafting plant height reaches 70-80cm and takes off film ridging, and ridging height is to cover grafting mouth about 6-8cm for degree. |
| process 4 | grafting plant height reaches 70-80cm and takes off film ridging, and ridging height is to cover grafting mouth about 8-10cm for degree. |
| contrast | own-rooted tree plant height reaches 70-80cm and takes off film, and ridging height is not to cover grafting mouth for degree. |
After flue-cured tobacco maturation, technology carries out picking and roasting routinely, statistics output, chooses upper leaf and measures nicotine content of tobacco leaves.
Table 2 grafting yield of flue-cured tobacco and nicotine content
Above-mentioned result of the test shows, grafting flue-cured tobacco difference ridging height, and its output is compared with conventional flue-cured tobacco, and difference is not remarkable; Nicotine content is with the increase of ridging height, and nicotine content also increases thereupon, thus can to a certain extent by changing ridging height, regulation and control nicotine content.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (9)
1. regulate and control a method for Nicotine Content in Tobacco Leaf, it is characterized in that, by the means of grafting with regulation and control transplanting time and height of earthing up are combined, the nicotine content of regulation and control tobacco.
2. method according to claim 1, is characterized in that, described method comprises the steps:
S1, utilize eggplant as stock, carry out the scion grafting of tobacco;
S2, grafting scion and stock heal completely, during newborn blade 1-2 sheet, by the transplantation of seedlings of grafting cigarette to land for growing field crops, and carry out plastic mulching, keep grafting mouth more than ground;
When S3, grafting plant height reach 70-80cm, carry out the ridging of differing heights, ridging height is not to cover grafting mouth or to cover grafting mouth 2cm ~ 10cm for degree.
3. method according to claim 2, is characterized in that, when obtaining nicotine content in advance and being the tobacco leaf of 0.002% ~ 0.005%, ridging is highly not cover grafting mouth for degree.
4. method according to claim 2, is characterized in that, when obtaining nicotine content in advance and being the tobacco leaf of 1.1% ~ 1.3%, ridging height is to cover grafting mouth 4 ~ 6cm for degree.
5. method according to claim 2, is characterized in that, when obtaining nicotine content in advance and being the tobacco leaf of 1.4% ~ 1.6%, ridging height is to cover grafting mouth 6 ~ 8cm for degree.
6. method according to claim 2, is characterized in that, when obtaining nicotine content in advance and being the tobacco leaf of 1.9% ~ 2.1%, ridging height is to cover grafting mouth 8 ~ 10cm for degree.
7. the method according to any one of claim 2 ~ 6, is characterized in that, described S1 comprises the steps:
(1) nursery: by eggplant seed and tobacco seed seeding and seedling raising;
(2) grafting: eggplant rootstock root is retained together with a part of stem stalk, and guarantee to leave 2 blades, remainder excises, by dark to incision 1 ~ 2cm for the stem top pars intermedia of eggplant rootstock; Scion root is excised, retains scion 4 leaf 1 heart, stem is whittled into long wedge, the long 1.5cm ~ 1.8cm of bevel; Tobacco scion is inserted the otch of eggplant rootstock, flue-cured tobacco scion and Solanum Torvum stock intimate surface are fitted, fixes with Grafting clip;
(3) managing after grafting: first 3 days after grafting, the shade net whole day of sheltering from heat or light rate with 85% was sheltered from heat or light, and at noon 10 ~ 14 time spraying to keep air humidity; Within 4th day, rise, before morning 9, after when afternoon 14, can shade net be opened, and between increasing gradually when ventilating and see light; Field run plant period management is proceeded to after 12 days.
8. method according to claim 7, is characterized in that, described eggplant seed is wild species Solanum Torvum seed.
9. method according to claim 7, is characterized in that, described step (1), by eggplant seed and tobacco seed seeding and seedling raising, is grown to 7 ~ 8 true leaves until eggplant seedling, tobacco seedling is when growing to 5 leaf 1 heart, eggplant seedling and tobacco seedling are taken out and prepare grafting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511030523.1A CN105532269B (en) | 2015-12-31 | 2015-12-31 | A kind of method of regulation and control Nicotine Content in Tobacco Leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511030523.1A CN105532269B (en) | 2015-12-31 | 2015-12-31 | A kind of method of regulation and control Nicotine Content in Tobacco Leaf |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105532269A true CN105532269A (en) | 2016-05-04 |
| CN105532269B CN105532269B (en) | 2018-06-01 |
Family
ID=55812279
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511030523.1A Active CN105532269B (en) | 2015-12-31 | 2015-12-31 | A kind of method of regulation and control Nicotine Content in Tobacco Leaf |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105532269B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171377A (en) * | 2016-06-30 | 2016-12-07 | 百色阳生农业开发有限公司 | A kind of cultural method improving outdoor cropping Tomato Grafting Seedling yield |
| CN109169124A (en) * | 2018-10-20 | 2019-01-11 | 河南农业大学 | The method for reducing content of nitrosamines in flue-cured tobacco |
| CN111713364A (en) * | 2019-03-20 | 2020-09-29 | 山东棉花研究中心 | Ridging method for relieving waterlogging damage of cotton |
| CN113141897A (en) * | 2021-04-29 | 2021-07-23 | 河南农业大学 | Tobacco grafting planting method for reducing nitrosamine content in tobacco |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030037986A (en) * | 2001-11-08 | 2003-05-16 | 전윤선 | A method for reducing amount of nicotin among components of leaf of Nicotiana Tobacum |
| CN102986439A (en) * | 2012-12-13 | 2013-03-27 | 湖北中烟工业有限责任公司 | Cultivating method for nicotine-reduced burley tobacco |
| CN103141311A (en) * | 2013-02-27 | 2013-06-12 | 湖北中烟工业有限责任公司 | Method for reducing burley tobacco nicotine |
| CN103782808A (en) * | 2014-01-16 | 2014-05-14 | 湖南省烟草公司湘西自治州公司 | Solanum melongena L. bacterial-wilt-resisting solanum melongena stock and grafting and cultivation method for grafting solanum melongena onto nicotiana |
-
2015
- 2015-12-31 CN CN201511030523.1A patent/CN105532269B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030037986A (en) * | 2001-11-08 | 2003-05-16 | 전윤선 | A method for reducing amount of nicotin among components of leaf of Nicotiana Tobacum |
| CN102986439A (en) * | 2012-12-13 | 2013-03-27 | 湖北中烟工业有限责任公司 | Cultivating method for nicotine-reduced burley tobacco |
| CN103141311A (en) * | 2013-02-27 | 2013-06-12 | 湖北中烟工业有限责任公司 | Method for reducing burley tobacco nicotine |
| CN103782808A (en) * | 2014-01-16 | 2014-05-14 | 湖南省烟草公司湘西自治州公司 | Solanum melongena L. bacterial-wilt-resisting solanum melongena stock and grafting and cultivation method for grafting solanum melongena onto nicotiana |
Non-Patent Citations (1)
| Title |
|---|
| 张春: "不同氮肥形态及培土对烤烟生长和烟碱积累的影响", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171377A (en) * | 2016-06-30 | 2016-12-07 | 百色阳生农业开发有限公司 | A kind of cultural method improving outdoor cropping Tomato Grafting Seedling yield |
| CN109169124A (en) * | 2018-10-20 | 2019-01-11 | 河南农业大学 | The method for reducing content of nitrosamines in flue-cured tobacco |
| CN111713364A (en) * | 2019-03-20 | 2020-09-29 | 山东棉花研究中心 | Ridging method for relieving waterlogging damage of cotton |
| CN113141897A (en) * | 2021-04-29 | 2021-07-23 | 河南农业大学 | Tobacco grafting planting method for reducing nitrosamine content in tobacco |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105532269B (en) | 2018-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101473747B (en) | Micro-graft and fast propagation method of tree | |
| Hemming et al. | The effect of diffuse light on crops | |
| CN102113445B (en) | Method for raising seedlings of Pinus kesiya var.langbianensis by efficient twig cuttage propagation | |
| CN102960174A (en) | Method for breeding and cultivating Taxus chinensis var mairei through asexual cuttage | |
| CN107135927B (en) | Cuttage and rapid propagation method for alum roots | |
| CN103875435B (en) | A kind of annual softwood cutting propagation method of plant leaf bud | |
| CN104542000A (en) | Cultivation method for camellia oleifera tree seedlings | |
| CN104756738A (en) | Cutting seedling method for twigs of elm of solar greenhouse | |
| CN105746224A (en) | Greenhouse seedling cultivating method for kalopanax septemlobus seeds | |
| CN105532269B (en) | A kind of method of regulation and control Nicotine Content in Tobacco Leaf | |
| CN104303788B (en) | It is a kind of that cane seedlings is floating heels in method | |
| CN110506620A (en) | A kind of greenhouse vegetable rock wool recycling method based on crop rotation | |
| KR101175895B1 (en) | Indoor cultivation germinating method of seeding of wood-cultivated ginseng | |
| CN100484392C (en) | Pregermination and seedling breeding method for blackberry with root | |
| CN104969776A (en) | Intensification root-breaking grafting and seedling cultivating method for thin-skinned melons | |
| CN102870579A (en) | Cutting propagation method of mono maple in seedling bags | |
| CN104067916A (en) | Greenhouse cuttage method of European yews | |
| CN106818225A (en) | A kind of tender anvil grafting rapid breeding method of Kiwi berry | |
| KR101870813B1 (en) | Method for enhancing rooting and growth of 'Maehyang' strawberry rooted cutting using over head irrigation or fog irrigation in summer season | |
| CN109729854A (en) | A kind of silk ball Jia Opulus (V. macrocephalum) cuttage breeding method | |
| CN111727753A (en) | Method for quickly growing seedlings and cultivating golden camellia | |
| CN105993522A (en) | Method for improving tulip tree cutting rooting survival rate | |
| CN109392648A (en) | A kind of high-quality strong seedling cultivation method of deer horn cuckoo | |
| CN101243759B (en) | Trunk-burying germination-forcing epicormic branch cuttage raise seedling technique | |
| Sandri et al. | High density of defoliated tomato plants in protected cultivation and its effects on development of trusses and fruits |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |