KR101555985B1 - Method for vegetative propagation of Populus species using micro-cutting - Google Patents
Method for vegetative propagation of Populus species using micro-cutting Download PDFInfo
- Publication number
- KR101555985B1 KR101555985B1 KR1020130102255A KR20130102255A KR101555985B1 KR 101555985 B1 KR101555985 B1 KR 101555985B1 KR 1020130102255 A KR1020130102255 A KR 1020130102255A KR 20130102255 A KR20130102255 A KR 20130102255A KR 101555985 B1 KR101555985 B1 KR 101555985B1
- Authority
- KR
- South Korea
- Prior art keywords
- cutting
- poplar
- micro
- roots
- propagation
- Prior art date
Links
- 238000005520 cutting process Methods 0.000 title claims abstract description 58
- 241000219000 Populus Species 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 239000010455 vermiculite Substances 0.000 claims description 4
- 235000019354 vermiculite Nutrition 0.000 claims description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 230000006698 induction Effects 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims description 2
- 229910001562 pearlite Inorganic materials 0.000 claims description 2
- 238000009395 breeding Methods 0.000 description 10
- 230000001488 breeding effect Effects 0.000 description 8
- 230000012010 growth Effects 0.000 description 7
- 241000255789 Bombyx mori Species 0.000 description 5
- 241000183024 Populus tremula Species 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 230000001850 reproductive effect Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000009313 farming Methods 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 239000003630 growth substance Substances 0.000 description 2
- 239000010451 perlite Substances 0.000 description 2
- 235000019362 perlite Nutrition 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000005082 stem growth Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 235000004035 Cryptotaenia japonica Nutrition 0.000 description 1
- 244000022759 Dacryodes edulis Species 0.000 description 1
- 235000004311 Dacryodes edulis Nutrition 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000037433 Pongamia pinnata Species 0.000 description 1
- 241000217825 Populus tremula x Populus tremuloides Species 0.000 description 1
- 240000004923 Populus tremuloides Species 0.000 description 1
- 241000218981 Populus x canadensis Species 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 102000007641 Trefoil Factors Human genes 0.000 description 1
- 235000015724 Trifolium pratense Nutrition 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000035584 blastogenesis Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 150000002013 dioxins Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000009894 physiological stress Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- OGFYIDCVDSATDC-UHFFFAOYSA-N silver silver Chemical compound [Ag].[Ag] OGFYIDCVDSATDC-UHFFFAOYSA-N 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G3/00—Cutting implements specially adapted for horticultural purposes; Delimbing standing trees
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S47/00—Plant husbandry
- Y10S47/03—Propagation of plant by cuttings
Abstract
본 발명은 휴면기(12월~3월)의 가지를 절취하여 미세 삽목(micro-cutting)의 방법으로 묘목을 육성하는, 미세 삽목을 이용한 포플러류 클론의 무성번식(vegetative propagation) 방법에 관한 것이다. The present invention relates to a vegetative propagation method of a poplar clone using a micro-cutting method in which a branch of a dormant period (December to March) is cut and a seedling is cultivated by a micro-cutting method.
Description
본 발명은 미세 삽목을 이용한 포플러류의 무성번식 방법에 관한 것으로, 보다 상세하게는 휴면기(12월~3월)의 가지를 절취하여 미세 삽목(micro-cutting)의 방법으로 묘목을 육성하는 무성번식(vegetative propagation) 방법에 관한 것이다.The present invention relates to a non-reproductive propagation method of poplar using micro-cutting, and more particularly, to a method of micro-cutting a seedling by cutting a branch of a dormant (December to March) (vegetative propagation) method.
국내에서의 포플러류 삽목발근 연구는 1970년대부터 시작되어 2000년 초반에 이르기 까지 다양한 연구가 수행되었다. Studies on the rooting of poplar cuttings in Korea have been carried out since the 1970s to the early 2000s.
朴貫圭는 은수원사시의 양묘법에서 삽목 시기는 해빙 후 일찍 시작하는 것이 좋으며 3월중에는 완료되어야 함을 밝히면서, 삽수길이는 17~20cm, 직경은 10~15mm의 것이 좋음을 제안하였다.Park suggested that the cutting time should be completed early in March and should be completed in March, while the cutting length should be 17 ~ 20cm and the diameter should be 10 ~ 15mm.
노의래(1982)는 현사시 품종과 새로운 양묘법을 소개하면서 11월 15일 전후에 실시하는 동계삽목법을 제안하였다. 구영본 등(1986)은 수원포플러의 삽목시험에서 삽수의 길이는 20cm, 직경은 11~20mm의 것을 사용하면 주간이나 측간을 삽수로 하여 삽목 증식에 문제가 없을 것으로 제안하였다. Noe Rae (1982) introduced a new type of oriental varieties and a new Yangmyeongmyeong, and suggested a new method of oriental sorting, which will be conducted around November 15. In Gyeongbyeongbuk et al. (1986), it was suggested that when the cutting length of 20cm and the diameter of 11 ~ 20mm were used in the cutting test of Suwon Poplar, cutting and cutting of the daytime and side were not problematic.
그 후 노의래 등(1988)은 사시나무의 뿌리 유래 맹아신초를 이용한 조직배양 대량증식법을 개발하였고, 그 후 1990년에는 사시나무의 여러 선발개체를 대상으로 근삽목을 실시하여 뿌리 길이는 25~30cm, 직경은 3~6cm의 근삽수를 사용하여 근매아 유도를 통해 3월에서 8월까지 연속적인 삽목이 가능하였고, 평균 삽목 발근율은 92% 이상 보였음을 보고하였다. Noh et al. (1988) developed a method for mass propagation of tissue culture using roots derived from roots of aspen trees. Then, in 1990, The average root cutting rate was more than 92%. The average root length was 30cm and the diameter was 3 ~ 6cm.
구영본 등(2001)은 미류남절 교잡종 포플러의 삽목에서 3월말 포지에 삽목을 통해 이태리포플러 I-214, Eco 28과 수원포플러 등의 생장과 내병성이 우수한 신품종을 선발 육성하였다. Gouyoungbong et al. (2001) selected and cultivated new varieties with excellent growth and tolerance, such as Poplar I-214, Eco 28 and Suwon Poplar from Italy, by cutting the Poplar at the end of March at the end of March.
외국에서의 포플러류 삽목증식 연구도 비교적 최근에 이르기까지 이루어지고 있다. Dolezelova 등(1996)은 Populus x canadensis M의 삽목시험에서 여러 가지 생장조절제 처리 후 발근에 미치는 내생 IAA의 농도변화를 조사하였다. Studies on the growth of poplar cuttings in foreign countries have been carried out relatively recently. Dolezelova et al. (1996) reported that Populus x canadensis M in the rooting root after treatment with various growth regulators.
Haapala 등 (2004)은 잡종사시나무(P. tremula x P. tremuloides)의 무성번식에서 녹지삽목법과 조직배양 기술적용이 효과적임을 보고하면서 삽수의 생장과 새로운 삽수의 생산력은 클론간 유이적 차이가 있음을 밝혔다. Stenvall 등(2006)은 5개의 잡종 사시나무의 삽목에서 삽수의 직경과 근삽수의 위치에 따라 맹아발생 및 발근율에 차이가 있음을 보고하였다. Haapala et al. (2004) reported that the application of green leaf-cutting and tissue culture techniques to silkworm propagation of P. tremula x P. tremuloides was effective, while the productivity of cuttings and the production of new cuttings varied between clones . Stenvall et al. (2006) reported that there was a difference in blastogenesis and rooting rate depending on the diameter of the cuttings and the position of the root cuttings in the cuttings of five hybrid aspen trees.
포플러 외의 다른 목본 수종에서도 삽목이나 조직배양에 의한 무성번식 기술이 최근까지 보고되고 있다. Mateja 등(2007)은 벚나무와 밤나무류의 엽삽목을 통한 생리적 연구에서 자동화 fogging system 하에서 삽수의 생리적 스트레스를 줄이는 것이 발근율 증진에 효과적임을 보고하였다. In other species of woody species other than poplar, silkworm breeding technique by cutting or tissue culture has been reported until recently. Mateja et al. (2007) reported that physiological studies using chopped and chestnut-leaf cuttings are effective in reducing the physiological stress of cutting under an automated fogging system.
Peternel 등(2009)은 유럽사시나무 (P. tremula L.)의 조직배양을 통한 효율적인 무성번식법을 발표하였다. Kesari 등(2009)는 바이오연료 생산수종으로 유망한 Pongamia pinnata (L.)의 우량목을 대상으로 줄기의 삽목에 미치는 몇 가지 오옥신의 종류 및 농도를 구명하였다. Peternel et al. (2009) published an efficient silent breeding method through tissue culture of European trefoil (P. tremula L.). Kesari et al. (2009) report that the promising biofuel producing species Pongamia Pinnata (L.) was used to investigate the types and concentrations of some dioxins on cuttings of the stem.
Ragonezi 등(2010)은 최근의 리뷰에서 침엽수종의 발근력 증진을 위해 일반삽수 및 조직배양묘의 미세 근(micro-shoot)을 재료로 발근에 미치는 생장조절제, 탄수화물, 빛의 종류, 온도, 발근촉진제, 배양토 등의 효과를 기술하였다. In a recent review, Ragonezi et al. (2010) reported that micro-shoots of seedlings in general cuttings and tissue cultures were used as growth regulators, carbohydrates, light type, temperature, rooting accelerator, And the effect of culture soil was described.
이상의 국내외 연구결과에서 보여준 것처럼 포플러의 삽목번식은 일반적으로 사용하는 효율적인 무성번식의 수단이 되어 왔으며, 수종에 따라 삽수의 크기, 발근제 처리, 삽목시기, 조직배양의 이용 등 다양한 방법이 개발되어 있다. As shown in the above domestic and overseas research results, the propagation of poplar has been an effective means of silent breeding generally used. Various methods have been developed depending on the species, such as the size of cutting, roots treatment, cutting time, .
현재까지 관행적으로 사용해 온 포플러류의 삽목 방법은 주로 휴면지를 이용한 노지삽목으로 삽목 시기는 3월 중순∼4월초(수원 기준)이었다. 그리고 삽목에 사용하는 삽수의 규격(크기)이 제한적이어서 가지의 직경이 1~1.5cm, 길이는 20~40cm 것을 사용하였고, 기타 잔가지는 발근이 어려워 사용하지 못하고 폐기하는 실정이었다.The cutting method of poplar tree which was used conventionally until now was mainly the use of dormant paddy and the cutting time was from mid March to early April (based on Suwon). The diameter of the branch was 1 ~ 1.5cm and the length was 20 ~ 40cm, because the size of the cutting used in the cutting was limited, and other twigs were discarded because they were difficult to roots.
상기한 바와 같이, 본 발명에서 제시된 것과 같은 미세 절단(micro-cutting)을 이용한 삽목번식법은 아직까지 개발되지 못하고 있다.
As described above, the method of breeding using micro-cutting as disclosed in the present invention has not yet been developed.
[참고문헌] [references]
박관규. 1975. 은수원사시의 양묘법. 포플러지 2권 pp 24Park, Kwan - Kyu. 1975. Yang-Hyeon-Seong's method of silver-silver radishes. Poplar paper 2 pp 24
노의래. 1982. 현사시 품종과 새로운 양묘법. 포플러지 5권 pp 41-46No answer. 1982. The varieties and the new Yangmyeongmyeon in the prefecture. Poplar paper 5 pp 41-46
구영본, 노의래, 이성규, 변광옥. 1986. 수원포플러 삽수형태 및 삽수 직경이 삽목묘의 생장과 Topophysis 형상에 미치는 영향. 임육연보 22: 15~20.Gyeongbyeong, Noe - rae, Lee, Sung - gyu, and Gwang - Ok. 1986. Influence of the shape and cutting diameter of Suwon Poplar on the growth and topophysis shape of Seedlings. 22: 15-20.
노의래, 이성규, 구영본, 정경호. 1988. 조직배양 및 삽목에 위한 우리나라 사시나무의 대량증식 방법. 임육연보 24: 20~27.Noe - Rae, Lee, Sung - Kyu, Koo, Young - Bong, and Jeong, Kyung - Ho. 1988. Mass proliferation method of Korean sashes for tissue culture and cutting. Annual Report of the Firms 24: 20-27.
노의래, 구영본, 이성규, 심상영, 심종섭. 1989. 우리나라 사시나무 유전자 보존 및 무성 대량증식에 관한 연구. 한국포플러위원회 pp 1~18.Noe - Rae, Koo Young - bong, Lee Sung - Kyu, Sang - Young Shim, Jong - Sup Shim. 1989. A Study on the Preservation and Silkworm Growth of Sash Tree in Korea. Korean Poplar Commission pp 1 ~ 18.
노의래, 구영본, 이성규, 심상영, 심종섭. 1990. 우리나라 사시나무 유전자 보존 및 무성대량 증식에 관한 연구. 포플러지 8권 pp 32-41. Noe - Rae, Koo Young - bong, Lee Sung - Kyu, Sang - Young Shim, Jong - Sup Shim. 1990. A Study on the Preservation and Silkworm Growth of the Sash Tree in Korea. Poplar paper 8 pp 32-41.
구영본, 김인식, 여진기, 이재천. 2001. 미루나무절(Aigeiros section) 교잡종 포플러 삽목묘의 생장특성.Koo Young Bong, Kim In Sik, Jin Ji Kee, Lee Jae Chun. 2001. Growth characteristics of poplar Seedlings of Aigeiros section hybrids.
DOLEZELOV T, PSOTA V and FEIGLOV Z. 1996. Endogenous indole-3-acetic acid during adventitious root formation in Populus canadensis Moench. BIOLOGIA PLANTARUM 38 (4): 617-619, 1996.DOLEZELOV T, PSOTA V and FEIGLOV Z. 1996. Endogenous indole-3-acetic acid during adventitious root formation in Populus canadensis Moench. BIOLOGIA PLANTARUM 38 (4): 617-619, 1996.
Stewart B. Rooda, Andrea R. Kalischuka, Mary Louise Polzina,and Jeffrey H. Braatneb. 2003. Branch propagation, not cladoptosis, permits dispersive, clonal reproduction of riparian cottonwoods. Forest Ecology and Management 186 (2003) 227242.Stewart B. Rooda, Andrea R. Kalischuka, Mary Louise Polzina, and Jeffrey H. Braatneb. 2003. Branch propagation, not cladoptosis, permits dispersive, clonal reproduction of riparian cottonwoods. Forest Ecology and Management 186 (2003) 227242.
Haapalaa T, Pakkanenb A, Pulkkinenb P. 2004. Variation in survival and growth of cuttings in two clonal propagation methods for hybrid aspen (Populus tremula , P. tremuloides). Forest Ecology and Management 193 (2004) 345354.Haapalaa T, Pakkanenba, Pulkkinenb P. 2004. Variation in survival and growth of two clonal propagation methods for hybrid aspen ( Populus tremula , P. tremuloides ). Forest Ecology and Management 193 (2004) 345354.
Stenvall N, Haapala T, Pulkkinen P. 2006. The role of a root cutting’s diameter and location on the regeneration ability of hybrid aspen Forest Ecology and Management 237: 150155.Stenvall N, Haapala T, and Pulkkinen P. 2006. The role of a root-cutting-diameter and location on the regeneration ability of hybrid aspen Forest Ecology and Management 237: 150155.
Mateja A., Dominik V., Franci S., Grefor O. 2007. The effects of a fogging system on the physiological status and rooting capacity of leafy cuttings of woody species. Trees 21: 491-496.Mateja A., Dominik V., Franci S., Grefor O. 2007. The effects of a fogging system on the physiological status and rooting capacity of leafy cuttings of woody species. Trees 21: 491-496.
Peternel S. et al. 2009. In vitro propagation of european aspen (Populus tremula L.) from axillary buds via organogenesis. Scientia Horticulturae. 121: 109-112.Peternel S. et al. 2009. In in vitro propagation of european aspen ( Populus tremula L.) from axillary buds via organogenesis. Scientia Horticulturae. 121: 109-112.
Kesari V., Krishnamachari A., Rangan L. 2009. Effect of auxins on adventitious rooting from cuttings of candidate plus tree Pongamia pinnata (L.), a potential biodiesel plant. Trees 23: 597-604.Kesari V., Krishnamachari A., Rangan L. 2009. Effect of auxins on adventitious rooting from the candidate plus tree Pongamia pinnata (L.), a potential biodiesel plant. Trees 23: 597-604.
Ragonezi C. et al. 2010. Adventitious rooting of conifers: influence of physical and chemical factors. Trees 24: 975-992.Ragonezi C. et al. 2010. Adventitious rooting of conifers: influence of physical and chemical factors. Trees 24: 975-992.
Asaah EK et al. 2012. Do propagation methods affect the fine root architecture of african plum (Dacryodes edulis) Trees 26: 1461-1469.Asaah EK et al. 2012. Do propagation methods affect the fine root architecture of african plum ( Dacryodes edulis ) Trees 26: 1461-1469.
Ziauka J. et al. 2013. Fast growing aspens in the development of a plant micropropagation system based on plant-produced ethylene action. Biomass and Bioenergy 53: 20-28.Ziauka J. et al. 2013. Fast growing aspens in the development of a plant micropropagation system based on plant-produced ethylene action. Biomass and Bioenergy 53: 20-28.
본 발명자들은 상기와 같은 종래기술의 한계를 해결하고자 지속적인 연구를 수행하여, 잔가지(직경 0.3~0.7cm, 길이 12~20cm)를 사용하여 포플러류의 새로운 삽목증식 기술을 개발하고, 삽목 시기도 12월부터 시작하여 이듬해 3월까지 실시하여 삽목묘 생산기간을 기존의 방법보다 2~4개월 앞당길 수 있음을 알아내고 본 발명을 완성하였다. The inventors of the present invention conducted a continuous study to solve the limitations of the prior art as described above, and developed a new cutting and propagating technology of poplar using twigs (diameter 0.3 to 0.7 cm, length 12 to 20 cm) Month from March to March of the following year, the seedling production period can be 2 to 4 months earlier than the conventional method, and the present invention has been completed.
따라서, 본 발명의 목적은 잔가지를 이용하여, 지금까지 개발되지 않은 미세 삽목(micro-cutting)을 이용한 포플러류의 무성번식 방법을 제공하는 것이다. Accordingly, it is an object of the present invention to provide a silent propagation method of poplar using micro-cutting which has not been developed so far using twigs.
본 발명은 상기와 같은 과제를 해결하기 위하여, (a) 삽수로서 휴면기에 있는 포플러류 클론의 당년생 줄기를 절단하는 단계, 및 (b) 상기 절단된 삽수를 삽목하는 단계를 포함하는, 잔가지로 삽목하는 미세 삽목(micro-cutting)을 이용한 포플러류의 무성번식 방법을 제공한다.In order to solve the above-mentioned problems, the present invention provides a method for producing a twig, comprising the steps of (a) cutting a perennial stem of a poplar clone in a dormant as an insert, and (b) The present invention provides a method for silent breeding of poplar using micro-cutting for cutting.
본 발명에 의한 미세 삽목을 이용한 포플러류의 무성번식 방법은 포플러류의 관행적인 삽목번식에서 그동안 사용하지 않았던 잔가지(직경 0.3~0.7cm)를 이용하는 장점이 있으며, 삽목 시기도 12월~3월에 실시할 수 있어 농한기에 유휴 인력을 활용할 수 있고, 시업 시기를 앞당길 수 있는 효과가 있다.The method of silkworm breeding of poplar using fine cutting according to the present invention is advantageous in that twigs (0.3-0.7 cm in diameter) which have not been used in conventional breeding of poplar are used, and the cutting time is also from December to March It is possible to utilize the idle manpower in the farming season, and it is effective to hasten the start time.
도 1은 순화실에서 미세 삽목(micro-cutting)된 포플러 발근 개체의 줄기생장 모습을 나타낸 것이다.
도 2는 대량으로 미세 삽목된 포플러류의 온실 생장 모습을 나타낸 것진이다. 미세 삽목된 포플러류는 삽목후 2개월이 되면 줄기가 20~50cm 로 자란다.
도 3은 발근된 개체의 줄기생장 및 뿌리의 상태를 나타낸 것이다.
도 4는 상기 도 3의 빌근된 뿌리 부분을 확대한 것이다.
도 5는 미세삽목으로 발근된 개체를 봄에 포지에 이식하여 3개월 정도 자란 묘목을 나타낸 것이다. Figure 1 shows the stem growth of micro-cut poplar roots in a purification chamber.
Fig. 2 is a graph showing the growth of a greenhouse of a large amount of micro-scaled poplar. Micro-scored poplar grows 20 ~ 50cm at 2 months after cutting.
Figure 3 shows stem growth and roots of rooted individuals.
FIG. 4 is an enlarged view of the root of the root of FIG. 3.
FIG. 5 shows seedlings grown for about 3 months after transplanting micro-germinated individuals into the forage in spring.
본 발명은 (a) 삽수로서 휴면기에 있는 포플러류 클론의 당년생 줄기를 절단하는 단계, 및 (b) 상기 절단된 삽수를 삽목하는 단계를 포함하는, 미세 삽목(micro-cutting)을 이용한 포플러류의 무성번식 방법에 관한 것이다.The present invention is directed to a method of making micro-cutting poplar products using micro-cutting, comprising the steps of: (a) cutting the perennial stem of a poplar clone in a dormant machine as an insert, and (b) Of the present invention.
본 발명에 의한 무성번식 방법은 상기 (a) 단계에서, 절단된 삽수에 발근제를 처리하는 단계를 더 포함할 수 있다. 상기 발근제 처리는 절단된 삽수의 기부에 처리하는 것이 바람직한데, 상기 발근제로는 예컨대, 1%(w/v)의 루톤등을 사용할 수 있다.The non-reproductive propagation method according to the present invention may further include the step of treating the rooting agent in the cutting operation in the step (a). The rooting agent treatment is preferably applied to the root of the cut end. As the rooting agent, for example, 1% (w / v) ruton may be used.
본 발명에 의한 무성번식 방법에서, 상기 (a) 단계의 절단은 상기 삽수의 기부를 사면으로 절단하고, 상기 (b) 단계의 삽목은 삽수를 수직으로 삽목하는 것이 바람직하지만, 상기 절단 및 삽수의 방향은 필요에 따라 변형될 수 있다.In the aseptic breeding method according to the present invention, it is preferable that cutting of the step (a) is performed by cutting the base portion of the above-mentioned cutting into slopes, and cutting of the step (b) The direction can be modified as needed.
본 발명에 의한 무성번식 방법에서, 상기 휴면기는 12월~3월이고, 상기 줄기의 직경은 0.3~0.7cm이며, 상기 절단하는 줄기의 길이는 12~20cm인 것이 바람직하지만, 상기 줄기의 직경 및 크기는 필요에 따라 변형될 수 있다. In the silent propagation method according to the present invention, the dormant is from December to March, the diameter of the stem is 0.3 to 0.7 cm, and the length of the cutting stalk is preferably 12 to 20 cm. However, The size can be modified as needed.
본 발명에 의한 무성번식 방법에서, 상기 삽목은 삽수를 피트모쓰, 펄라이트 및 버미큘라이트가 등량 용적비로 혼합되어 있는 인공혼합 상토에 삽목하는 것이 바람직하지만, 피트모쓰, 펄라이트 및 버미큘라이트의 혼합비는 필요에 따라 변형될 수 있다. In the silent propagation method according to the present invention, it is preferable that the cutting is carried out in an artificial mixed soil in which the pit moth, pearlite and vermiculite are mixed at an equivalent volume ratio, but the mixing ratio of pit moss, perlite and vermiculite .
본 발명에 의한 무성번식 방법은 상기 (b) 단계 후에 삽목된 삽수에 관수하고, 15~25℃의 온도로 유지되는 순화 온실에서 상토가 건조하지 않도록 관수하여 발근을 유도하는 단계를 더 포함할 수 있다. The non-reproductive propagation method according to the present invention may further include the step of irrigating the roots in the roasting greenhouse kept at a temperature of 15 to 25 ° C by watering the roots after the step (b) have.
본 발명에 의한 무성번식 방법은 발근이 유도된 개체를 포지로 이식하여 묘목으로 육성하는 단계를 더 포함할 수 있다. 이때, 상기 발근이 유도된 개체는 삽목후 2개월 정도 경과한 것을 사용할 수 있고, 상기 발근된 개체는 봄에 포지로 이식하여 정상적인 묘목으로 육성시킬 수 있다. The aseptic propagation method according to the present invention may further include a step of transplanting the root induction-inducing body into a plant and cultivating it as a seedling. At this time, the root-induced root can be used for about 2 months after the rooting, and the rooted root can be transplanted into the root in the spring and can be grown as a normal seedling.
이하 본 발명을 실시예를 통하여 보다 상세하게 설명한다. 그러나, 이들 실시예는 본 발명을 예시적으로 실시하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.
Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.
<실시예 1>≪ Example 1 >
포플러류 양버들 등 20종의 클론을 대상으로 수원의 산림과학원 산림유전자원부 포플러류 채수포에서 2013년 1월에, 휴면기에 당년생의 직경 0.5cm의 줄기를 취하여, 상기 줄기의 길이가 16cm이고, 기부가 사면으로 되도록 절단하여 삽수를 제조하였다.A total of 20 clones, such as poplar, were harvested in January 2013 from Poplar flowering in Suweon Forestry and Forestry Ministry of Forestry and Forestry in Suwon. The length of the stalk was 16cm, The base was cut to make a slope, and the cutting was made.
상기 20종의 클론은 62-7, 97-18, 97-19, 97-12, 양버들, Ay48, 벨로또(Bellotto), 보깔라리(Boccalari), 카파세이오(Carpaceio), 도스캠프(Dorskamp), Eco28, 구아디(Guardi), I-214, I-476, 엘. 아방조(L. Avanzo), 미루나무, 산마르티노(San Martino), V-211, V-435 및 벤지아노(Venziano)이었다. The 20 clones were identified as 62-7, 97-18, 97-19, 97-12, soya beans, Ay48, Bellotto, Boccalari, Carpaceio, Dorskamp, , Eco28, Guardi, I-214, I-476, EL. L. Avanzo, Cottonwood, San Martino, V-211, V-435 and Venziano.
상기 절단된 삽수의 기부에 발근제로 1%(w/v)의 루톤(동부한농(주))을 분의처리(seed dressing)한 후, 상기 발근제 처리된 삽수를 피트모쓰, 펄라이트 및 버미큘라이트가 등량 용적비로 혼합되어 있는 인공혼합 상토에 수직으로 삽목하였다.After the seed dressing of 1% (w / v) of ruton (Dongbu Hanon Co., Ltd.) as a rooting agent was applied to the base of the cutter, the rooting agent treated pit moth, perlite and vermiculite The slices were cut vertically into an artificial mixed soil mixed at an equivalent volume ratio.
상기 삽목된 삽수에 관수하고, 15~25℃의 온도로 유지되는 순화 온실에서 상토가 건조되지 않도록 매일 1회씩 간헐적인 관수를 하고, 배양 3주 후에는 2~3일에 한 번씩 관수하여 발근을 유도하였다.In the cleaned greenhouse maintained at a temperature of 15 to 25 캜, water was sprinkled intermittently once a day to prevent the soil from drying, and after 3 weeks of cultivation, water was irrigated once every 2 to 3 days. Respectively.
삽목 후 2개월 정도 경과하여 20cm 이상의 길이로 자란 발근된 개체를 굴취하여 봄에 포지로 이식하고 육성시켜, 정상적인 클론묘목으로 육성하였다. Two months after cutting, the rooted individuals grown to a length of 20 cm or longer were transplanted into the forage in spring and cultivated in the spring, and cultivated as normal clone seedlings.
본 발명에 의한 무성번식 방법은 포플러류의 관행적인 삽목번식에서 그동안 사용하지 않았던 잔가지를 이용하는 장점이 있으며, 삽목 시기도 12월~3월에 실시할 수 있어 농한기에 유휴 인력을 활용할 수 있고, 시업 시기를 앞당길 수 있기 때문에, 산업적으로 유용하게 사용될 수 있다.The method of silent breeding according to the present invention is advantageous in that the twigs that have not been used in conventional breeding of poplar have been used, and the cutting time can also be performed from December to March, so that idle manpower can be utilized in the farming period, It can be used industrially because it can advance the time.
Claims (7)
(b). 상기 절단된 삽수를 삽목하되, 상기 삽목은 상기 절단된 삽수에 1%(w/v)의 발근제를 처리한 후, 상기 발근제가 처리된 삽수를 피트모쓰, 펄라이트 및 버미큘라이트가 등량 용적비로 혼합되어 있는 인공혼합 상토에 삽목하는 단계; 및
(c). 상기 삽목된 삽수에 관수하고, 15~25℃의 온도로 유지되는 순화 온실에서 상토가 건조하지 않도록 관수하여 발근을 유도하는 단계를 포함하는, 잔가지로 삽목하는 미세 삽목(micro-cutting)을 이용한 포플러류 클론의 무성번식 방법.(a). Cutting a perennial stem of a poplar clone in a dormant cavity into a twig with a diameter of 0.5 cm and a length of 16 cm in January as a cutting, cutting the base of the cutting into slopes; And
(b). The cuts were cut and the cuts were treated with 1% (w / v) rooting agent in the cuts, and then the rooting agent treated was mixed with equivalent amounts of pitmoss, pearlite and vermiculite ≪ / RTI > And
(c). Cutting the roots with a twig, comprising the step of irrigating the roots of the roots so as to prevent the roots from drying out in a purifying greenhouse maintained at a temperature of 15 to 25 캜, The method of silent propagation of the clone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130102255A KR101555985B1 (en) | 2013-08-28 | 2013-08-28 | Method for vegetative propagation of Populus species using micro-cutting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130102255A KR101555985B1 (en) | 2013-08-28 | 2013-08-28 | Method for vegetative propagation of Populus species using micro-cutting |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20150025092A KR20150025092A (en) | 2015-03-10 |
| KR101555985B1 true KR101555985B1 (en) | 2015-09-30 |
Family
ID=53021402
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020130102255A KR101555985B1 (en) | 2013-08-28 | 2013-08-28 | Method for vegetative propagation of Populus species using micro-cutting |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR101555985B1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180105983A (en) | 2017-03-16 | 2018-10-01 | 대한민국(산림청 국립산림과학원장) | Highly sensitive determining method of Populus trees for stress tolerance and its use |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134806B (en) * | 2016-08-22 | 2019-12-10 | 新疆林科院造林治沙研究所 | Grafting propagation method for natural hybrid populus grey in whole growing season |
| CN106717975B (en) * | 2017-01-05 | 2018-06-29 | 任泽楷 | A kind of cuttage breeding method of Dunnia sinensis |
| KR102183725B1 (en) * | 2019-01-15 | 2020-11-27 | 철원군 | Plant propagation method of larch seedlings by planting |
| CN111226704B (en) * | 2020-03-12 | 2022-03-18 | 惠州市九惠制药股份有限公司 | Cutting seedling raising method for savatier shawense |
| WO2023192474A1 (en) * | 2022-04-01 | 2023-10-05 | Benson Hill Company | Method to produce seeds rapidly through asexual propagation of cuttings in legumes |
-
2013
- 2013-08-28 KR KR1020130102255A patent/KR101555985B1/en active IP Right Grant
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180105983A (en) | 2017-03-16 | 2018-10-01 | 대한민국(산림청 국립산림과학원장) | Highly sensitive determining method of Populus trees for stress tolerance and its use |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20150025092A (en) | 2015-03-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101555985B1 (en) | Method for vegetative propagation of Populus species using micro-cutting | |
| CN105613062B (en) | A kind of rubber select tree tender tip grafting childrenization method for culturing seedlings | |
| Banik | A manual for vegetative propagation of bamboos | |
| CN100571503C (en) | A kind of elaeagnus mollis asexual reproduction method | |
| CN101563996A (en) | Jiuqian plum tree engrafting and cultivating method | |
| CN106879369B (en) | A kind of tea tree Special germplasm resources grafting quick-breeding method | |
| CN101911899A (en) | Method for grafting various trees onto fingered citron stocks | |
| CN103609295A (en) | Method for large-scale cultivation of jatropha curcas seedlings | |
| CN108967014A (en) | A kind of fast rapid-result garden method for downgrading apple | |
| WO2015131751A1 (en) | Method of establishing cutting nursery for large-scale propagation of american sequoia seedling | |
| CN101828485A (en) | Method for improving cold-resistant property of Korla fragrant pears by high-position grafting | |
| CN104798606A (en) | Method for grafting michelia champaca in top grafting manner by using michelia chapensis dandies as parental stocks | |
| CN106034722A (en) | Cutting propagation method of golden leaf plant namely weigela florida variegata | |
| CN105746282B (en) | Rubber tree budling nursery proliferation and rejuvenation thereafter and method for changing | |
| CN105493906A (en) | Orange seedling grafting method | |
| CN104429480A (en) | Taxodium zhongshansha 406 hard wood cutting simple culture method | |
| CN107173083A (en) | A kind of Apples Dwarf Stocks grafting seedlings-growing method | |
| CN107027627A (en) | A kind of micro-tuber propagation method of David's-harp IMMATURE EMBRYOS CULTURE | |
| CN106234110A (en) | A kind of Phoebe bournei grafting seedlings-growing method | |
| CN110622815B (en) | Method for cultivating camellia miniascape by two-step grafting method of camellia oleifera tree root | |
| Dhillon et al. | Development of efficient techniques for clonal multiplication of Jatropha curcas L., a potential biodiesel plant | |
| CN106508386A (en) | Interplanting method of taxus chinensis var.mairei and prunella vulgaris L | |
| CN109997538A (en) | A kind of wax plum layering seedling growth method | |
| CN107494168A (en) | A kind of ligustrum lucidum ait shoot buds connect propagation method | |
| CN111357510B (en) | Cuttage propagation method for carpinus tientaiensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| AMND | Amendment | ||
| E601 | Decision to refuse application | ||
| AMND | Amendment | ||
| X701 | Decision to grant (after re-examination) | ||
| GRNT | Written decision to grant |