CN103080337B - 利用回文序列生产闭合线性dna - Google Patents
利用回文序列生产闭合线性dna Download PDFInfo
- Publication number
- CN103080337B CN103080337B CN201180038378.5A CN201180038378A CN103080337B CN 103080337 B CN103080337 B CN 103080337B CN 201180038378 A CN201180038378 A CN 201180038378A CN 103080337 B CN103080337 B CN 103080337B
- Authority
- CN
- China
- Prior art keywords
- dna
- seq
- protelomerase
- template
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1013153.0A GB201013153D0 (en) | 2010-08-04 | 2010-08-04 | Primer for production of closed linear DNA |
| GB1013153.0 | 2010-08-04 | ||
| PCT/GB2011/001175 WO2012017210A1 (en) | 2010-08-04 | 2011-08-04 | Production of closed linear dna using a palindromic sequence |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103080337A CN103080337A (zh) | 2013-05-01 |
| CN103080337B true CN103080337B (zh) | 2016-08-17 |
Family
ID=42931209
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180038378.5A Active CN103080337B (zh) | 2010-08-04 | 2011-08-04 | 利用回文序列生产闭合线性dna |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9499847B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2601312B1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP2013535210A (cg-RX-API-DMAC7.html) |
| CN (1) | CN103080337B (cg-RX-API-DMAC7.html) |
| GB (1) | GB201013153D0 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2012017210A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (79)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0901593D0 (en) * | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
| EP2692870A1 (en) * | 2012-08-03 | 2014-02-05 | Alacris Theranostics GmbH | Method for nucleic acid amplification |
| AU2014233150A1 (en) | 2013-03-15 | 2015-09-17 | Theranos Ip Company, Llc | Nucleic acid amplification |
| WO2014145298A1 (en) | 2013-03-15 | 2014-09-18 | Theranos, Inc. | Nucleic acid amplification |
| ES2738602T3 (es) | 2013-03-15 | 2020-01-24 | Theranos Ip Co Llc | Amplificación de ácidos nucleicos |
| US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
| US9290800B2 (en) | 2013-03-15 | 2016-03-22 | Pacific Biosciences Of California, Inc. | Targeted rolling circle amplification |
| MX356055B (es) | 2013-09-06 | 2018-05-11 | Theranos Ip Co Llc | Sistemas y métodos para detectar enfermedades infecciosas. |
| EP3957735A1 (en) | 2014-03-05 | 2022-02-23 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating usher syndrome and retinitis pigmentosa |
| US11339437B2 (en) | 2014-03-10 | 2022-05-24 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
| EP3116997B1 (en) | 2014-03-10 | 2019-05-15 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating leber's congenital amaurosis 10 (lca10) |
| US11141493B2 (en) | 2014-03-10 | 2021-10-12 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
| WO2015148863A2 (en) | 2014-03-26 | 2015-10-01 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating sickle cell disease |
| EP3540061A1 (en) | 2014-04-02 | 2019-09-18 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating primary open angle glaucoma |
| GB201415789D0 (en) * | 2014-09-05 | 2014-10-22 | Touchlight Genetics Ltd | Synthesis of DNA |
| GB201502645D0 (en) * | 2015-02-17 | 2015-04-01 | Touchlight Genetics Ltd | Method |
| EP4019975A1 (en) | 2015-04-24 | 2022-06-29 | Editas Medicine, Inc. | Evaluation of cas9 molecule/guide rna molecule complexes |
| FR3042121A1 (fr) * | 2015-10-08 | 2017-04-14 | Jean-Marc Limacher | Composition anti-tumorale |
| US11512311B2 (en) | 2016-03-25 | 2022-11-29 | Editas Medicine, Inc. | Systems and methods for treating alpha 1-antitrypsin (A1AT) deficiency |
| JP7004651B2 (ja) * | 2016-07-19 | 2022-02-04 | 株式会社バイオダイナミクス研究所 | 長鎖一本鎖ｄｎａを調製する方法 |
| US11566263B2 (en) | 2016-08-02 | 2023-01-31 | Editas Medicine, Inc. | Compositions and methods for treating CEP290 associated disease |
| ES2813324T3 (es) * | 2016-08-16 | 2021-03-23 | Touchlight Ip Ltd | Producción de ADN lineal cerrado |
| WO2018170184A1 (en) | 2017-03-14 | 2018-09-20 | Editas Medicine, Inc. | Systems and methods for the treatment of hemoglobinopathies |
| EP3622070A2 (en) | 2017-05-10 | 2020-03-18 | Editas Medicine, Inc. | Crispr/rna-guided nuclease systems and methods |
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| US10350307B2 (en) * | 2017-09-18 | 2019-07-16 | General Electric Company | In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides |
| EP3701028B8 (en) | 2017-10-24 | 2024-10-23 | Editas Medicine, Inc. | Systems and methods for treating hyper-igm syndrome |
| EP3707266A1 (en) | 2017-11-07 | 2020-09-16 | Editas Medicine, Inc. | Targeted integration systems and methods for the treatment of hemoglobinopathies |
| AU2018383712B2 (en) | 2017-12-11 | 2025-09-04 | Editas Medicine, Inc. | Cpf1-related methods and compositions for gene editing |
| WO2019118806A1 (en) * | 2017-12-14 | 2019-06-20 | Solid Biosciences Inc. | Non-viral production and delivery of genes |
| SG11202008956XA (en) | 2018-03-14 | 2020-10-29 | Editas Medicine Inc | Systems and methods for the treatment of hemoglobinopathies |
| CN108531496B (zh) * | 2018-04-04 | 2020-11-06 | 江南大学 | 一种提高外源基因mRNA数量的DNA及其应用 |
| GB201805683D0 (en) | 2018-04-05 | 2018-05-23 | Touchlight Ip Ltd | Reprogramming vectors |
| EP3810782A2 (en) * | 2018-06-22 | 2021-04-28 | Asklepios Biopharmaceutical, Inc. | Vectors for gene delivery that persist within cells |
| WO2020254876A1 (en) | 2019-06-18 | 2020-12-24 | Janssen Sciences Ireland Unlimited Company | Virus-like particle delivery of hepatitis b virus (hbv) vaccines |
| US20220305107A1 (en) | 2019-06-18 | 2022-09-29 | Janssen Sciences Ireland Unlimited Company | COMBINATION OF HEPATITIS B VIRUS (HBV) VACCINES AND HBV-TARGETING RNAi |
| WO2020255010A1 (en) | 2019-06-18 | 2020-12-24 | Janssen Sciences Ireland Unlimited Company | Combination of recombinant interleukin 12 construct and hepatitis b virus (hbv) vaccines |
| KR20220042116A (ko) | 2019-06-18 | 2022-04-04 | 얀센 사이언시즈 아일랜드 언리미티드 컴퍼니 | B형 간염 바이러스(HBV) 백신 및 HBV-타케팅 RNAi의 조합 |
| EP3986915A1 (en) | 2019-06-18 | 2022-04-27 | Janssen Sciences Ireland Unlimited Company | Recombinant interleukin 12 construct and uses thereof |
| CA3143634A1 (en) | 2019-06-18 | 2020-12-24 | Janssen Sciences Ireland Unlimited Company | Combination of hepatitis b virus (hbv) vaccines and anti-pd-1 antibody |
| JP2022536850A (ja) | 2019-06-18 | 2022-08-19 | ヤンセン・サイエンシズ・アイルランド・アンリミテッド・カンパニー | Ｂ型肝炎ウイルス（ｈｂｖ）ワクチンおよび抗ｐｄ－１または抗ｐｄ－ｌ１抗体の組合せ |
| US20220305108A1 (en) | 2019-06-20 | 2022-09-29 | Janssen Sciences Ireland Unlimited Company | Lipid nanoparticle or liposome delivery of hepatitis b virus (hbv) vaccines |
| AU2020297019A1 (en) | 2019-06-20 | 2022-02-17 | Janssen Sciences Ireland Unlimited Company | Carbohydrate nanocarrier delivery of hepatitis B virus (HBV) vaccines |
| EP3792367A1 (en) | 2019-09-11 | 2021-03-17 | Universität Bielefeld | Method for the production of raav and method for the in vitro generation of genetically engineered, linear, single-stranded nucleic acid fragments containing itr sequences flanking a gene of interest |
| CA3151464A1 (en) | 2019-09-18 | 2021-03-25 | Bruce C. SCHNEPP | Synthetic dna vectors and methods of use |
| GB201915163D0 (en) | 2019-10-18 | 2019-12-04 | Univ Southampton | Cancer vaccine |
| AU2021212319A1 (en) * | 2020-01-31 | 2022-07-28 | Tyris Therapeutics, S.L. | Process for the production of closed linear DNA |
| GB202007428D0 (en) | 2020-05-19 | 2020-07-01 | Fabricnano Ltd | Polynucleotide synthesis |
| AU2021289600A1 (en) * | 2020-06-12 | 2023-01-19 | University Of Rochester | Encoding and expression of ACE-tRNAs |
| CN112111544B (zh) * | 2020-09-23 | 2022-04-01 | 复旦大学附属肿瘤医院 | 提高单链dna连接效率的方法 |
| WO2022156188A1 (en) * | 2021-01-22 | 2022-07-28 | Nanjing GenScript Biotech Co., Ltd. | Method for producing target dna sequence and cloning vector |
| ES2960777T3 (es) * | 2021-03-18 | 2024-03-06 | Genomill Health Oy | Métodos para la cuantificación paralela precisa de ácidos nucleicos en muestras diluidas o no purificadas |
| GB202108176D0 (en) | 2021-06-08 | 2021-07-21 | Touchlight Ip Ltd | Vector |
| JP2024520797A (ja) | 2021-06-08 | 2024-05-24 | タッチライト・アイピー・リミテッド | レンチウイルスベクター |
| US20230055381A1 (en) | 2021-06-25 | 2023-02-23 | Oxford Biomedica Solutions Llc | Adeno-associated virus packaging systems |
| EP4370151A1 (en) | 2021-07-13 | 2024-05-22 | Stonehaven Incubate AG | Composition and uses thereof |
| GB202114105D0 (en) | 2021-10-01 | 2021-11-17 | Fabricnano Ltd | Nucleotide synthesis |
| WO2023069948A1 (en) | 2021-10-18 | 2023-04-27 | Flagship Pioneering Innovations Vii, Llc | Dna compositions and related methods |
| US20230323395A1 (en) | 2021-12-15 | 2023-10-12 | Homology Medicines, Inc. | Methods and compositions for the production of adeno-associated virus |
| JP2025503470A (ja) | 2021-12-20 | 2025-02-04 | ゾエティス・サービシーズ・エルエルシー | 細胞膜上に抗原を発現させるための組成物及び方法 |
| EP4453008A1 (en) | 2021-12-20 | 2024-10-30 | Zoetis Services LLC | Compositions and methods for prevention of piscine myocarditis |
| MX2024007696A (es) | 2021-12-20 | 2024-07-01 | Zoetis Services Llc | Uso del interferon como adyuvante en vacunas. |
| CN114561372B (zh) * | 2022-04-27 | 2022-07-26 | 南京巨匠生物科技有限公司 | Bst DNA聚合酶突变体及其应用和产品、基因、重组质粒和基因工程菌 |
| EP4293101A1 (en) | 2022-06-14 | 2023-12-20 | Asklepios Biopharmaceutical, Inc. | Reactor with temperature control and method of using the same |
| CN119698477A (zh) * | 2022-08-10 | 2025-03-25 | 镇江蓬勃生物科技有限公司 | 线性闭合dna的制备方法及该方法所用质粒 |
| WO2024058155A1 (ja) * | 2022-09-12 | 2024-03-21 | 株式会社カネカ | 二本鎖環状ｄｎａベクター、直鎖状共有結合閉鎖ｄｎａの作製方法、並びにプロテロメラーゼ及びエンドヌクレアーゼを含む融合ポリペプチド |
| JP2025530305A (ja) | 2022-09-12 | 2025-09-11 | ゾエティス・サービシーズ・エルエルシー | 水産養殖用ワクチンの投与方法 |
| CN117778498A (zh) * | 2022-09-26 | 2024-03-29 | 深圳瑞吉生物科技有限公司 | 一种无细胞制备mRNA转录模板的方法 |
| EP4593877A1 (en) | 2022-09-29 | 2025-08-06 | Ceva Hampton Limited | Vaccine construct and uses thereof |
| WO2024121354A1 (en) * | 2022-12-08 | 2024-06-13 | Keygene N.V. | Duplex sequencing with covalently closed dna ends |
| DK202370610A1 (en) | 2022-12-13 | 2024-07-12 | Zoetis Services Llc | Methods of vaccine administration to salmonids |
| WO2024197081A1 (en) | 2023-03-20 | 2024-09-26 | Hc Bioscience, Inc. | Minimally-sized dna threads for trna therapy |
| CN116606834A (zh) * | 2023-04-10 | 2023-08-18 | 天津中合基因科技有限公司 | 多种具有切割连接活性的原端酶及其应用 |
| WO2024256842A1 (en) | 2023-06-16 | 2024-12-19 | Touchlight IP Limited | Therapeutic transfection |
| US11981936B1 (en) | 2023-09-29 | 2024-05-14 | New England Biolabs, Inc. | TelA variants, compositions, and methods |
| GB202319998D0 (en) | 2023-12-22 | 2024-02-07 | Touchlight Ip Ltd | duplexing method |
| FR3160186A1 (fr) | 2024-03-13 | 2025-09-19 | Odimma Therapeutics | Vecteur non-viral pour transcription augmentee |
| WO2025202929A1 (en) * | 2024-03-29 | 2025-10-02 | Pfizer Inc. | Methods for producing nucleic acids |
| WO2025250742A1 (en) * | 2024-05-30 | 2025-12-04 | Aclarity Genomics, Inc. | Amplification of macromolecules |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001077384A2 (de) * | 2000-04-07 | 2001-10-18 | Epigenomics Ag | DETEKTION VON SNPs UND CYTOSIN-METHYLIERUNGEN |
| CN101103122A (zh) * | 2004-12-11 | 2008-01-09 | 西托吉尼克斯公司 | 高质量核酸的无细胞生物合成及其用途 |
Family Cites Families (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5703055A (en) | 1989-03-21 | 1997-12-30 | Wisconsin Alumni Research Foundation | Generation of antibodies through lipid mediated DNA delivery |
| JPH03236781A (ja) * | 1990-02-13 | 1991-10-22 | Takara Shuzo Co Ltd | ランダムプライマーキット及びその使用方法 |
| AU649066B2 (en) | 1990-07-25 | 1994-05-12 | Syngene, Inc. | Circular extension for generating multiple nucleic acid complements |
| FR2675803B1 (fr) | 1991-04-25 | 1996-09-06 | Genset Sa | Oligonucleotides fermes, antisens et sens et leurs applications. |
| US6953676B1 (en) | 1992-05-01 | 2005-10-11 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US5714320A (en) | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
| EP0636413B1 (en) | 1993-07-28 | 2001-11-14 | PE Corporation (NY) | Nucleic acid amplification reaction apparatus and method |
| CA2175380A1 (en) * | 1993-10-29 | 1995-05-04 | Lan Bo Chen | A novel tumor marker and novel method of isolating same |
| FR2732971B1 (fr) | 1995-04-13 | 1997-07-04 | Genset Sa | Oligonucleotide sens inhibiteur de virus herpes simplex (hsv) a structure en haltere |
| US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| JP3974941B2 (ja) | 1995-11-21 | 2007-09-12 | イェール ユニバーシティ | 単分子セグメントの増幅および検出 |
| DE19648625A1 (de) | 1996-11-13 | 1998-05-14 | Soft Gene Gmbh | Mikroprojektil für das Einbringen von Substanzen in Zellen durch ballistischen Transfer |
| JPH10234399A (ja) * | 1997-02-28 | 1998-09-08 | Shinkinrui Kinou Kaihatsu Kenkyusho:Kk | ピシウム属菌検出用の核酸配列 |
| US6054274A (en) * | 1997-11-12 | 2000-04-25 | Hewlett-Packard Company | Method of amplifying the signal of target nucleic acid sequence analyte |
| AU758407B2 (en) | 1997-12-24 | 2003-03-20 | Cepheid | Integrated fluid manipulation cartridge |
| CA2317896C (en) | 1998-01-09 | 2004-08-10 | University Of Utah Research Foundation | Method for in vitro amplification of circular dna |
| DE19826758C1 (de) | 1998-06-15 | 1999-10-21 | Soft Gene Gmbh | Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte |
| WO2000015779A2 (en) | 1998-09-15 | 2000-03-23 | Yale University | Molecular cloning using rolling circle amplification |
| AU6683400A (en) | 1999-07-08 | 2001-01-30 | Mologen Forschungs-, Entwicklungs- Und Vertriebs Gmbh | Vaccine against lentiviral infections, such as the feline immune deficiency virus of the cat |
| GB0018120D0 (en) | 2000-07-24 | 2000-09-13 | Fermentas Ab | Nuclease |
| JP2002051784A (ja) * | 2000-08-09 | 2002-02-19 | Toyobo Co Ltd | 核酸の増幅方法及び標識核酸の調製方法 |
| US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
| US20030077611A1 (en) | 2001-10-24 | 2003-04-24 | Sention | Methods and systems for dynamic gene expression profiling |
| US20030170693A1 (en) * | 2001-12-21 | 2003-09-11 | George Chaconas | Assay for identifying modulators of Borrelia telomere resolvase |
| US7081339B2 (en) | 2002-04-12 | 2006-07-25 | Primera Biosystems, Inc. | Methods for variation detection |
| AU2003251905A1 (en) | 2002-07-12 | 2004-02-02 | Affymetrix, Inc. | Synthetic tag genes |
| WO2004028562A2 (de) | 2002-09-23 | 2004-04-08 | Mologen Ag | Impfstoff gegen infektionen mit onkoviren, wie dem felinen leukosevirus der katze |
| US7955795B2 (en) | 2003-06-06 | 2011-06-07 | Qiagen Gmbh | Method of whole genome amplification with reduced artifact production |
| US7452699B2 (en) | 2003-01-15 | 2008-11-18 | Dana-Farber Cancer Institute, Inc. | Amplification of DNA in a hairpin structure, and applications |
| EP1594987B1 (en) | 2003-02-21 | 2009-05-27 | GeneForm Technologies Limited | Nucleic acid sequencing methods, kits and reagents |
| JP2007512020A (ja) | 2003-11-26 | 2007-05-17 | エッペンドルフ アクチェンゲゼルシャフト | 染色体外核酸のインビトロ増幅のための方法及び組成物 |
| JP3972106B2 (ja) * | 2004-03-03 | 2007-09-05 | 大学共同利用機関法人情報・システム研究機構 | ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー |
| EP1819828A2 (en) * | 2004-12-11 | 2007-08-22 | CytoGenix, Inc. | Cell free biosynthesis of high-quality nucleic acid and uses thereof |
| EP1871897B1 (en) | 2005-04-12 | 2010-09-29 | Olink AB | Methods for production of oligonucleotides |
| DE602006015633D1 (de) | 2005-04-29 | 2010-09-02 | Synthetic Genomics Inc | Amplifikation und klonierung einzelner dna-moleküle mittels rolling-circle-amplifikation |
| WO2007087478A2 (en) | 2006-01-12 | 2007-08-02 | Lucigen Corporation | Linear vectors, host cells and cloning methods |
| WO2008045288A2 (en) | 2006-10-06 | 2008-04-17 | Vandalia Research, Inc. | Method for a continuous rapid thermo cycle system |
| US20080118917A1 (en) * | 2006-11-21 | 2008-05-22 | Applera Corporation | Isothermal SNP Detection Method |
| US8921072B2 (en) * | 2008-09-02 | 2014-12-30 | General Electric Compnay | Methods to generate DNA mini-circles |
| GB0901593D0 (en) * | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
| JP5182949B2 (ja) * | 2009-03-31 | 2013-04-17 | 浜名部品工業株式会社 | ヨークの製造方法及び製造装置 |
| US9114399B2 (en) | 2010-08-31 | 2015-08-25 | Canon U.S. Life Sciences, Inc. | System and method for serial processing of multiple nucleic acid assays |
| EP2692870A1 (en) | 2012-08-03 | 2014-02-05 | Alacris Theranostics GmbH | Method for nucleic acid amplification |
| KR102014989B1 (ko) | 2013-04-15 | 2019-08-27 | 삼성전자주식회사 | 폴리뉴클레오티드 및 그의 용도 |
-
2010
- 2010-08-04 GB GBGB1013153.0A patent/GB201013153D0/en not_active Ceased
-
2011
- 2011-08-04 CN CN201180038378.5A patent/CN103080337B/zh active Active
- 2011-08-04 JP JP2013522292A patent/JP2013535210A/ja active Pending
- 2011-08-04 US US13/814,106 patent/US9499847B2/en active Active
- 2011-08-04 WO PCT/GB2011/001175 patent/WO2012017210A1/en not_active Ceased
- 2011-08-04 EP EP11746284.6A patent/EP2601312B1/en active Active
-
2016
- 2016-10-14 JP JP2016202277A patent/JP6689726B2/ja active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001077384A2 (de) * | 2000-04-07 | 2001-10-18 | Epigenomics Ag | DETEKTION VON SNPs UND CYTOSIN-METHYLIERUNGEN |
| CN101103122A (zh) * | 2004-12-11 | 2008-01-09 | 西托吉尼克斯公司 | 高质量核酸的无细胞生物合成及其用途 |
Non-Patent Citations (1)
| Title |
|---|
| Linear closed mini DNA generated by the prokaryotic cleaving-joining enzyme TelN is functional in mammalian cells;Jochen Heinrich et al.;《Journal of Molecular Medicine》;20080828;第80卷(第10期);摘要，第649页第2-6段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| GB201013153D0 (en) | 2010-09-22 |
| WO2012017210A1 (en) | 2012-02-09 |
| JP2013535210A (ja) | 2013-09-12 |
| US20130216562A1 (en) | 2013-08-22 |
| JP2017035102A (ja) | 2017-02-16 |
| JP6689726B2 (ja) | 2020-04-28 |
| EP2601312B1 (en) | 2017-04-12 |
| EP2601312A1 (en) | 2013-06-12 |
| CN103080337A (zh) | 2013-05-01 |
| US9499847B2 (en) | 2016-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103080337B (zh) | 利用回文序列生产闭合线性dna | |
| JP6454243B2 (ja) | 閉鎖型直鎖状ｄｎａの製造 | |
| US11149302B2 (en) | Method of DNA synthesis | |
| JP2022530476A (ja) | 等温ｄｎａ増幅のための方法及び組成物 | |
| US12509718B2 (en) | Method of DNA synthesis | |
| HK1215045A1 (zh) | 用於制备闭合线状dna的体外无细胞方法的试剂盒 | |
| HK1159693B (en) | Production of closed linear dna |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220120 Address after: London Patentee after: TOUCHLIGHT GENETICS LTD. Address before: London Patentee before: TOUCHLIGHT GENETICS Ltd. |
|
| TR01 | Transfer of patent right |