CN103074293A - 一种mdck细胞制备流感疫苗的培养基及其使用方法 - Google Patents
一种mdck细胞制备流感疫苗的培养基及其使用方法 Download PDFInfo
- Publication number
- CN103074293A CN103074293A CN2012105950942A CN201210595094A CN103074293A CN 103074293 A CN103074293 A CN 103074293A CN 2012105950942 A CN2012105950942 A CN 2012105950942A CN 201210595094 A CN201210595094 A CN 201210595094A CN 103074293 A CN103074293 A CN 103074293A
- Authority
- CN
- China
- Prior art keywords
- substratum
- large scale
- high density
- mdck cell
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229960003971 influenza vaccine Drugs 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 title claims description 19
- 239000001963 growth medium Substances 0.000 title abstract 2
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 241000700605 Viruses Species 0.000 claims description 25
- 210000002966 serum Anatomy 0.000 claims description 17
- 241000712461 unidentified influenza virus Species 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 244000309466 calf Species 0.000 claims description 11
- 238000011081 inoculation Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 230000010412 perfusion Effects 0.000 claims description 7
- 239000002435 venom Substances 0.000 claims description 5
- 210000001048 venom Anatomy 0.000 claims description 5
- 231100000611 venom Toxicity 0.000 claims description 5
- 229940124873 Influenza virus vaccine Drugs 0.000 claims description 4
- 238000005374 membrane filtration Methods 0.000 claims description 2
- 230000009385 viral infection Effects 0.000 claims description 2
- 210000003734 kidney Anatomy 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 31
- 239000000243 solution Substances 0.000 description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 239000001301 oxygen Substances 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- 230000010261 cell growth Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 239000003570 air Substances 0.000 description 4
- 235000011089 carbon dioxide Nutrition 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 210000004102 animal cell Anatomy 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 229920002307 Dextran Polymers 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- VEZXCJBBBCKRPI-UHFFFAOYSA-N beta-propiolactone Chemical compound O=C1CCO1 VEZXCJBBBCKRPI-UHFFFAOYSA-N 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920001606 poly(lactic acid-co-glycolic acid) Polymers 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229910021426 porous silicon Inorganic materials 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 229960000380 propiolactone Drugs 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
0天 | 1天 | 2天 | 3天 | 4天 | 5天 | |
实施例1 | 5 | 45.1 | 100.5 | 178.3 | 294 | 525.5 |
实施例2 | 5 | 40.5 | 98.9 | 167.5 | 268.4 | 511.3 |
实施例3 | 5 | 38.2 | 83.1 | 150.8 | 273.6 | 486 |
实施例4 | 5 | 31.1 | 76.2 | 135.3 | 250.2 | 455.6 |
实施例5 | 5 | 35.9 | 91.2 | 173.9 | 288.1 | 503.2 |
MEM+15%FBS | 5 | 10.3 | 30.4 | 63.5 | 89.1 | 105.1 |
DMEM+15%FBS | 5 | 11.5 | 35.1 | 72.2 | 103.7 | 122.8 |
1天 | 2天 | 3天 | 4天 | 5天 | 6天 | 7天 | 8天 | 9天 | 10天 | |
实施例1 | 5.5 | 6.8 | 7.3 | 8.4 | 8.8 | 9.0 | 9.3 | 9.2 | 8.9 | 8.5 |
实施例2 | 5.8 | 6.4 | 7.7 | 8.2 | 8.5 | 8.9 | 9.5 | 9.0 | 8.5 | 8.1 |
实施例3 | 5.4 | 6.1 | 7.2 | 8.1 | 8.6 | 8.8 | 9.1 | 9.2 | 8.6 | 8.0 |
实施例4 | 5.2 | 5.9 | 7.5 | 8.2 | 8.4 | 8.6 | 9.3 | 9.0 | 8.8 | 7.8 |
实施例5 | 5.9 | 6.6 | 7.4 | 8.0 | 8.6 | 9.2 | 9.8 | 9.6 | 9.0 | 8.5 |
DMEM/F12 | 4.2 | 5.1 | 5.9 | 6.5 | 7.1 | 7.5 | 7.0 | 6.7 | 6.2 | 5.6 |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210595094.2A CN103074293B (zh) | 2012-12-28 | 2012-12-28 | 一种mdck细胞制备流感疫苗的培养基及其使用方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210595094.2A CN103074293B (zh) | 2012-12-28 | 2012-12-28 | 一种mdck细胞制备流感疫苗的培养基及其使用方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103074293A true CN103074293A (zh) | 2013-05-01 |
CN103074293B CN103074293B (zh) | 2015-07-22 |
Family
ID=48150992
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201210595094.2A Active CN103074293B (zh) | 2012-12-28 | 2012-12-28 | 一种mdck细胞制备流感疫苗的培养基及其使用方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103074293B (zh) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104232564A (zh) * | 2014-05-12 | 2014-12-24 | 杭州国牧生物科技有限公司 | 一种st细胞制备猪瘟疫苗的培养基及其使用方法 |
CN104232565A (zh) * | 2014-05-12 | 2014-12-24 | 杭州国牧生物科技有限公司 | 一种mpk细胞制备猪瘟疫苗的培养基及其使用方法 |
CN104278009A (zh) * | 2014-09-12 | 2015-01-14 | 江苏省农业科学院 | 无血清培养基及其用途和一种猪瘟病毒的培养方法 |
CN104894057A (zh) * | 2015-06-25 | 2015-09-09 | 江苏太一生物科技有限公司 | 一种mrc-5细胞制备狂犬病疫苗的培养基及其使用方法 |
CN111304169A (zh) * | 2019-12-03 | 2020-06-19 | 北京亚东生物制药有限公司 | 一种培养基添加物、含有该添加物的细胞培养基及其应用 |
CN114561364A (zh) * | 2022-03-02 | 2022-05-31 | 上海荣盛生物药业股份有限公司 | 高效扩增人流感病毒的方法和试剂 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101245335A (zh) * | 2008-03-21 | 2008-08-20 | 乐能生物工程股份有限公司 | 无血清植物蛋白肽通用细胞培养基 |
CN101914485A (zh) * | 2010-08-05 | 2010-12-15 | 乐能生物工程股份有限公司 | 一种无血清大豆蛋白肽动物细胞培养基的配制方法 |
-
2012
- 2012-12-28 CN CN201210595094.2A patent/CN103074293B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101245335A (zh) * | 2008-03-21 | 2008-08-20 | 乐能生物工程股份有限公司 | 无血清植物蛋白肽通用细胞培养基 |
CN101914485A (zh) * | 2010-08-05 | 2010-12-15 | 乐能生物工程股份有限公司 | 一种无血清大豆蛋白肽动物细胞培养基的配制方法 |
Non-Patent Citations (2)
Title |
---|
张建伟: "不同培养基对H9亚型禽流感病毒在MDCK细胞上的增殖效果研究", 《安徽农业科学》, 31 August 2012 (2012-08-31), pages 4609 - 4611 * |
网友: "5种MEM细胞培养基成分", 《生物帮》, 13 April 2012 (2012-04-13), pages 1 - 3 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104232564A (zh) * | 2014-05-12 | 2014-12-24 | 杭州国牧生物科技有限公司 | 一种st细胞制备猪瘟疫苗的培养基及其使用方法 |
CN104232565A (zh) * | 2014-05-12 | 2014-12-24 | 杭州国牧生物科技有限公司 | 一种mpk细胞制备猪瘟疫苗的培养基及其使用方法 |
CN104278009A (zh) * | 2014-09-12 | 2015-01-14 | 江苏省农业科学院 | 无血清培养基及其用途和一种猪瘟病毒的培养方法 |
CN104278009B (zh) * | 2014-09-12 | 2017-11-17 | 江苏省农业科学院 | 无血清培养基及其用途和一种猪瘟病毒的培养方法 |
CN104894057A (zh) * | 2015-06-25 | 2015-09-09 | 江苏太一生物科技有限公司 | 一种mrc-5细胞制备狂犬病疫苗的培养基及其使用方法 |
CN111304169A (zh) * | 2019-12-03 | 2020-06-19 | 北京亚东生物制药有限公司 | 一种培养基添加物、含有该添加物的细胞培养基及其应用 |
CN111304169B (zh) * | 2019-12-03 | 2022-03-18 | 北京亚东生物制药有限公司 | 一种培养基添加物、含有该添加物的细胞培养基及其应用 |
CN114561364A (zh) * | 2022-03-02 | 2022-05-31 | 上海荣盛生物药业股份有限公司 | 高效扩增人流感病毒的方法和试剂 |
Also Published As
Publication number | Publication date |
---|---|
CN103074293B (zh) | 2015-07-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103074293B (zh) | 一种mdck细胞制备流感疫苗的培养基及其使用方法 | |
CN102816732B (zh) | 适应全悬浮无血清培养的mdck细胞系及其在培养流感病毒、生产流感病毒疫苗中的应用 | |
CN101979518B (zh) | 一种制备伪狂犬病病毒的方法 | |
CN104232564A (zh) | 一种st细胞制备猪瘟疫苗的培养基及其使用方法 | |
CN101851610A (zh) | 一种大规模生产动物用狂犬病毒抗原的方法 | |
CN101979517B (zh) | 一种利用生物反应器大规模生产流感病毒的方法 | |
CN101869702B (zh) | 以悬浮微载体细胞培养系统产制的疫苗及其方法 | |
CN101716341B (zh) | 一种人用二倍体细胞狂犬灭活疫苗及其制备方法 | |
CN101979514B (zh) | 猪蓝耳病病毒与疫苗及二者的生产方法 | |
CN107460156A (zh) | 无血清全悬浮mdck细胞株及其在生产流感病毒中的应用 | |
CN103394081B (zh) | 利用wave波浪生物反应器生产禽流感疫苗的方法 | |
CN108220227A (zh) | 一种通过全悬浮传代细胞系悬浮培养新城疫病毒的方法 | |
CN107904215A (zh) | 一种禽流感病毒的全悬浮培养方法 | |
CN104338127A (zh) | 一种h9n2亚型禽流感灭活疫苗的生产方法及其制品 | |
CN103087995B (zh) | 一种使用bhk-21贴壁细胞制备口蹄疫疫苗的方法 | |
CN102807964A (zh) | 一种动物细胞放大培养的方法 | |
CN103520715B (zh) | 利用wave波浪生物反应器生产猪圆环病毒2型灭活疫苗的方法 | |
CN103409364A (zh) | 一种pk15细胞制备猪圆环ⅱ型病毒疫苗的培养基及其使用方法 | |
CN102886043B (zh) | 猪乙型脑炎病毒与猪细小病毒二联灭活疫苗及其制备方法 | |
CN103285385B (zh) | 一种制备猪圆环病毒2型灭活疫苗的方法 | |
CN107794248A (zh) | 一种用微载体悬浮培养犬瘟热病毒的方法 | |
CN104109652A (zh) | 人工诱导四倍体鲫鱼细胞系及其培养方法和应用 | |
Katinger et al. | Status and developments of animal cell technology using suspension culture techniques | |
CN107432931B (zh) | 一种微载体培养水痘病毒制备疫苗的方法 | |
CN102260650A (zh) | 鸡马立克氏病毒的大规模生产方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
DD01 | Delivery of document by public notice |
Addressee: Jia Xindan Document name: the First Notification of an Office Action |
|
DD01 | Delivery of document by public notice | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20180601 Address after: 230601 Hefei Chuang Hua Industrial Park, Anhui, China, 2F218 351 Patentee after: Hefei Level Biotechnology Co., Ltd. Address before: 310053 2 floor B2216 room, six and 368 Road, Binjiang District, Hangzhou, Zhejiang. Patentee before: Hangzhou Guomu Bio-Technology Co., Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20191108 Address after: 214000 no.8410, zone B, science and technology entrepreneurship Park, No.16, Changjiang Road, Xinwu District, Wuxi City, Jiangsu Province Patentee after: Jiangsu Taiyi Biotechnology Co., Ltd. Address before: 230601 Hefei Chuang Hua Industrial Park, Anhui, China, 2F218 351 Patentee before: Hefei Level Biotechnology Co., Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20200813 Address after: Room 501, building 1, No. 619, BINKANG Road, Binjiang District, Hangzhou City, Zhejiang Province Patentee after: HANGZHOU GUOMU BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. Address before: 214000 no.8410, zone B, science and technology entrepreneurship Park, No.16, Changjiang Road, Xinwu District, Wuxi City, Jiangsu Province Patentee before: JIANGSU TAIYI BIOTECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right |