CN103069009B - 环介导等温扩增(lamp)的序列特异性实时监测 - Google Patents

环介导等温扩增(lamp)的序列特异性实时监测 Download PDF

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CN103069009B
CN103069009B CN201180040896.0A CN201180040896A CN103069009B CN 103069009 B CN103069009 B CN 103069009B CN 201180040896 A CN201180040896 A CN 201180040896A CN 103069009 B CN103069009 B CN 103069009B
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probe
lamp
oligonucleotide chain
reaction
reaction mixture
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CN103069009A (zh
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久保田亮
丹尼尔·M·詹金斯
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University of Hawaii at Hilo
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201180040896.0A 2010-06-22 2011-06-22 环介导等温扩增(lamp)的序列特异性实时监测 Expired - Fee Related CN103069009B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35742810P 2010-06-22 2010-06-22
US61/357,428 2010-06-22
PCT/US2011/041540 WO2011163425A1 (en) 2010-06-22 2011-06-22 Sequence specific real-time monitoring of loop-mediated isothermal amplification (lamp)

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CN103069009A CN103069009A (zh) 2013-04-24
CN103069009B true CN103069009B (zh) 2015-01-14

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Country Link
US (1) US20130171643A1 (enrdf_load_stackoverflow)
EP (1) EP2585617B1 (enrdf_load_stackoverflow)
JP (1) JP5984802B2 (enrdf_load_stackoverflow)
KR (1) KR101832011B1 (enrdf_load_stackoverflow)
CN (1) CN103069009B (enrdf_load_stackoverflow)
AU (1) AU2011270885B2 (enrdf_load_stackoverflow)
BR (1) BR112012032519A2 (enrdf_load_stackoverflow)
CA (1) CA2803161C (enrdf_load_stackoverflow)
MX (1) MX2012014917A (enrdf_load_stackoverflow)
WO (1) WO2011163425A1 (enrdf_load_stackoverflow)

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US9074249B2 (en) 2012-06-04 2015-07-07 New England Biolabs, Inc. Detection of amplification products
US9074243B2 (en) 2012-07-27 2015-07-07 New England Biolabs, Inc. Detection of amplification products
CN103540660B (zh) * 2013-10-10 2015-10-28 中国人民解放军疾病预防控制所 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒
CN103602727B (zh) * 2013-10-25 2015-03-11 中国林业科学研究院热带林业研究所 一种快速检测青枯菌的检测引物组、检测试剂盒和检测方法
US10106837B1 (en) 2014-02-05 2018-10-23 Steven A Benner Processes for point of care detection of DNA and RNA
KR101496835B1 (ko) 2014-03-04 2015-02-27 서울대학교산학협력단 살모넬라균 검출을 위한 등온증폭반응용 프라이머 조성물 및 이를 이용한 살모넬라균의 검출 방법
US10913973B2 (en) 2014-09-17 2021-02-09 Board Of Regents, The University Texas System Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification
US10724091B1 (en) 2015-02-10 2020-07-28 National Technology & Engineering Solutions Of Sandia, Llc Endpoint detection of amplified nucleic acids
KR101552159B1 (ko) 2015-03-10 2015-09-10 경기대학교 산학협력단 고리-매개 등온 증폭법을 이용한 진균성 질병 검출 방법
CN104745694A (zh) * 2015-03-17 2015-07-01 苏州华麦生物科技有限公司 一种转基因水稻科丰6的恒温探针法检测引物组、检测试剂盒及检测方法
CN104946760B (zh) * 2015-06-16 2017-12-08 河南省农业科学院 一种检测肿囊腐霉的方法及专用试剂盒
KR101875662B1 (ko) * 2015-12-01 2018-08-02 에스디 바이오센서 주식회사 등온 핵산 증폭을 위한 리포터 염료, 퀀처가 포함되는 등온 기반 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭과 측정 방법
ITUB20159332A1 (it) 2015-12-22 2017-06-22 Diasorin S P A Procedimento di rivelazione in fluorescenza dell?amplificazione isotermica mediata da loop (LAMP) di un acido nucleico bersaglio, relativi oligonucleotidi e kit.
WO2018027236A1 (en) * 2016-08-05 2018-02-08 Arizona Board Of Regents On Behalf Of Arizona State University Methods for digital readout quantification of nucleic acids
EP3290527B1 (en) * 2016-08-29 2019-06-19 MetaSystems Indigo GmbH Method for detecting microorganisms in a sample
US20210395810A1 (en) * 2016-11-30 2021-12-23 Steven A Benner Processes to Detect Coronaviruses
US11366116B1 (en) 2017-04-12 2022-06-21 National Technology & Engineering Solutions Of Sandia, Llc Real time autonomous surveillance of pathogens
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
SG10201910254YA (en) * 2019-11-04 2021-06-29 Denka Company Ltd Nucleic Acid Detection Method Using Lamp And Probe Detection
KR102687405B1 (ko) * 2020-10-16 2024-07-23 경북대학교 산학협력단 돼지 써코바이러스 3형 검출용 조성물 및 이를 이용한 돼지 써코바이러스 3형 검출 방법
KR20240104144A (ko) 2021-12-10 2024-07-04 주식회사 씨젠 단일 가닥 상호작용적 이중 표지 시스템을 포함하는 태그된 루프프라이머 및 이를 이용한 핵산 등온 증폭 방법
CN114196769B (zh) * 2021-12-27 2024-03-08 中国科学院长春应用化学研究所 一种用于检测沙门氏菌的引物、试剂盒及其使用方法
WO2024005458A1 (ko) 2022-06-27 2024-01-04 주식회사 씨젠 베타-하이드록시산을 이용한 타겟 핵산의 증폭 방법
CN117867152B (zh) * 2024-03-08 2024-05-24 云南英茂现代农业有限公司 一种用于检测青枯菌的核酸引物组、包含其的产品及应用

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KR101832011B1 (ko) 2018-02-23
EP2585617A4 (en) 2013-11-27
MX2012014917A (es) 2013-03-18
JP2013530705A (ja) 2013-08-01
CA2803161C (en) 2019-09-24
JP5984802B2 (ja) 2016-09-07
CN103069009A (zh) 2013-04-24
CA2803161A1 (en) 2011-12-29
AU2011270885B2 (en) 2016-05-19
HK1184830A1 (zh) 2014-01-30
US20130171643A1 (en) 2013-07-04
WO2011163425A1 (en) 2011-12-29
BR112012032519A2 (pt) 2017-07-18
KR20130044303A (ko) 2013-05-02
AU2011270885A1 (en) 2013-01-10
EP2585617A1 (en) 2013-05-01
EP2585617B1 (en) 2016-05-11

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