CN103005210A - Pig feed additive capable of replacing antibiotics, and preparation method and application thereof - Google Patents

Pig feed additive capable of replacing antibiotics, and preparation method and application thereof Download PDF

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CN103005210A
CN103005210A CN2012105697554A CN201210569755A CN103005210A CN 103005210 A CN103005210 A CN 103005210A CN 2012105697554 A CN2012105697554 A CN 2012105697554A CN 201210569755 A CN201210569755 A CN 201210569755A CN 103005210 A CN103005210 A CN 103005210A
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parts
phosphoric acid
feed additive
pig feed
carrier
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CN103005210B (en
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姚继明
范文君
罗士津
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Guangdong Wangda Group Co ltd
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Guangzhou Wangda Biological Technology Co Ltdluogang Branch
JIANGXI WANGDA ANIMAL TECHNOLOGY Co Ltd
GUANGZHOU WANGDA FEED TECHNOLOGY Co Ltd
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Abstract

The invention relates to a pig feed additive capable of replacing antibiotics, and a preparation method and an application thereof. Components and weight part ratio thereof in a formula are as follows: 1-5 parts of medicated leaven, 1-5 parts of angelica sinensis, 1-5 parts of radix astragali, 1-5 parts of hawthorn, 1-5 parts of radix codonopsis, 10-20 parts of citric acid, 10-20 parts of lactic acid, 20-30 parts of coated phosphoric acid, 5-10 parts of a micro-ecological preparation, 5-10 parts of yeast cell wall polysaccharide, 1-5 parts of a composite plant essential oil and 5-10 parts of a carrier. The pig feed additive carries out combination collocation of traditional Chinese herbs and functional polysaccharide, the micro-ecological preparation, acidifying agents, plant essential oil and the like, so that synergic effect of the traditional Chinese herbs and other components are maximized; the pig feed additive can replace the antibiotics completely; problems of drug resistance, drug residues, overcommitment and the like of the antibiotics which trouble animal husbandry for a long time is reduced; and at the same time, the pig feed additive plays effects of improving culture environment, reducing feed safety hidden dangers, increasing culture benefits and the like.

Description

A kind of alternative antibiotic pig feed additive and its preparation method and application
Technical field
The present invention designs a kind of additive of pig feed, more specifically refers to a kind of alternative antibiotic pig feed additive and its preparation method and application.
Background technology
Begin from the forties, aquaculture begins progressively to be widely used antibiotic, for huge impetus has been played in the animal husbandry fast development, but along with long-term, the antibiotic use of high dose, has also produced following problem:
The long-term antibiotic that uses makes animal body produce a large amount of antibody-resistant bacterium, has strengthened the treatment difficulty, even can't treat, and causes mortality of animals high, and the cultivation loss is larger, more is unfavorable for the development that animal husbandry is healthy, optimum; One period residence time is arranged in animal body after the antibiotic metabolism, if eaten by the people this moment, then can affect body immunity and premunition, long-term taking can cause immune function of human body disorderly, is burying huge hidden danger aspect the epidemic disease control; Aquaculture still has a large amount of casual households and the intensive producer on a small scale, and the supervision difficulty is large, and supervision exists leak and blank, even strengthen regulatory efforts, can not fundamentally solve the residual problem such as higher of abuse of antibiotics, medicine.
For this reason, European Union, the U.S., Japan and other countries a series of compulsory measures that release one after another limit or forbid adding antibiotic in feeds.Fast development along with China's animal husbandry, the antibiotic problem progressively causes extensive attention, China begins progressively antibiotic interpolation kind and use amount in the limiting feed already, " nonreactive " feed has become the main trend of following animal husbandry development, for this reason, the research and development of Substitutes For Antibiotic have become the focus of animal husbandry benign development.
There is at present the technology of some productions " nonreactive " feed addictive mostly to be the combination of full Chinese herbal medicine or complete non-Chinese herbal medicine, number be 200410084602.6 such as Chinese patent application, patent name is pure Chinese medicine pig's feed additive, it is to be ground into respectively powder by the Radix Astragali, spina date seed, rhizome of cyrtomium, wilsonii, the root of herbaceous peony, Fructus Hordei Germinatus, Divine Comedy and dried orange peel, then mix and make, be full Chinese herbal medicine combination in this technical scheme, do not take full advantage of the synergy between Chinese herbal medicine and the non-Chinese herbal medicine.
Number be 201110361980.4 such as Chinese patent application, invention and created name is feed addictive of a kind of substitute antibiotics and its preparation method and application, this application discloses a kind of by the microbial ecological preparation, functional oligosaccharide, the feed addictive of the substitute antibiotics that organic acid and carrier form, the multiplex Bacillus acidi lactici of probiotics and bacillus subtilis in the product mix of the substitute antibiotics in this application, Bacillus acidi lactici non-refractory wherein, loss late is at 30%-60% in the mixed feed pelletization, and Bacillus acidi lactici resistance is poor, in culture presevation, all brought certain trouble in the transportation; Employed bacillus subtilis is comparatively ripe in the prior art, but can not lactic acid producing, and its producing bacillus subtilis product gemma rate of the producer of Market reaction 30%-40% is lower, has greatly affected result of use; Simultaneously, what acidulant adopted in the product mix of above-mentioned substitute antibiotics is lactic acid and citric acid, and the effect that full organic acid combination reduces the pig stomach inner pH value is slightly poor; Simple organic acid does not have the effects such as slowly-releasing in the stomach, lasting reduction pig stomach inner pH value without what be coated with.
Summary of the invention
Main purpose of the present invention is the problems such as a series of residual such as drug resistance, medicine, the excess that causes for antibiotic in the pig feed use procedure, a kind of pig feed additive of complete substitute antibiotics is provided.
To achieve these goals, the present invention has adopted following technical scheme:
A kind of alternative antibiotic pig feed additive, component and parts by weight thereof in its prescription are:
Divine Comedy 1-5 part, Radix Angelicae Sinensis 1-5 part, Radix Astragali 1-5 part, hawthorn 1-5 part, Radix Codonopsis 1-5 part; Citric acid 10-20 part, lactic acid 10-20 part, coated phosphoric acid 20-30 part, probiotics 5-10 part, yeast cell wall polysaccharide 5-10 part, composite plant essential oil 1-5 part and carrier 5-10 part.
Wherein, probiotics comprises following composition: 1.0 * 10 8～1.0 * 10 9The bafillus natto of CFU/g, 1.0 * 10 8～1.0 * 10 9The CFU/g bacillus that condenses, bafillus natto is 1:1-10:1 with the weight ratio of the bacillus that condenses.
Wherein, the composite plant essential oil comprises following composition and mass percent: Thymol: 1-2%, and geraniol: 0.5-1%, bergamot oil: 9-11%, surplus is vegetable oil and absorbent-type carrier.
Coated phosphoric acid is the mixture of list/bi-tristearin and phosphoric acid, and the weight ratio of described list/bi-tristearin and described phosphoric acid is 0.5-0.65:1.
Described carrier is inorganic or organic carrier, and described organic carrier comprises maize cob meal or maltodextrin, and inorganic carrier comprises white carbon or medical stone.
Wherein, Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis are Chinese herbal medicine, have antiviral, enhancing immunoregulation effect; Citric acid, lactic acid, coated phosphoric acid then have the effect of regulating hydrochloric acid in gastric juice, reducing the gastric juice pH value; Bafillus natto, these the two kinds of probioticses of bacillus that condense have regulating intestinal canal health, promote the intestinal mucosa reparation, suppress the harmful bacteria growth, improve the effect of feeding environment, and the yeast cell wall polysaccharide then can the stimulating animal immune system, improve animal health condition, reduce the Animal stress reaction; The composite plant essential oil can promote pig search for food, antibiotic, pig immunity, anti-body oxidative function be provided.
A kind of preparation method of alternative antibiotic pig feed additive comprises the steps, wherein umber is parts by weight:
1), 20-80 purpose Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis are carried out respectively 300 order ultramicro grinding broken wall treatment, sporoderm-broken rate is more than 80%, Divine Comedy 1-5 part, Radix Angelicae Sinensis 1-5 part, Radix Astragali 1-5 part, hawthorn 1-5 part, Radix Codonopsis 1-5 part of taking by weighing by weight after the broken wall treatment fully mix 140-160 second again, and it is for subsequent use to make composite Chinese herbal;
2) list of a certain amount of solid sheet/bi-tristearin is added in the stainless steel cauldron, insulation after being heated to 82-87 ℃, again 85% food-class liquid phosphoric acid is added in the stainless steel cauldron, keep reaction temperature at 82-87 ℃, speed of agitator 480-520r/min, the reaction time is 110-130min, make coated phosphoric acid, and place the heatproof Plastic Drum interior for subsequent use, wherein, the weight ratio of list/bi-tristearin and described phosphoric acid is 0.5-0.65:1;
3) take by weighing citric acid 10-20 part, lactic acid 10-20 part, coated phosphoric acid 20-30 part and carrier 1-2 part, use double helix cone-type mixer mixing 9-11min, put into baking car pallet after fully mixing, change in the heated-air circulation oven and dry, bake out temperature is 46-50 ℃, time 5.5-6.5h, the coated phosphoric acid that adopts again broken vibrations sieving machine to dry, lactic acid, citric acid and carrier mixture are pulverized, it is for subsequent use to make compound acidulant after sieving, wherein, and coated phosphoric acid, lactic acid, granularity after citric acid and carrier mixture are pulverized is greater than 80 orders;
4) with probiotics 5-10 part, yeast cell wall polysaccharide 5-10 part, composite plant essential oil 1-5 part and carrier 1-2 part stir fully that to make functional raw material behind the mixed 170-190S for subsequent use;
5) composite Chinese herbal, compound acidulant, functional raw material and the 3-6 part carrier that makes placed in the three-dimensional motion mixer mix, incorporation time is that 200-220S namely makes alternative antibiotic pig feed additive.
Wherein, probiotics comprises following composition: 1.0 * 10 8～1.0 * 10 9The bafillus natto of CFU/g, 1.0 * 10 8～1.0 * 10 9The CFU/g bacillus that condenses, the weight ratio of bafillus natto and the bacillus that condenses is 1:1-10:1.
A kind of application of alternative antibiotic pig feed additive is used for alternative antibiotic pig feed additive is added into swine feed by the addition of 3-4kg/T.
Employed bafillus natto is high temperature resistant, acidproof, alkaline-resisting in the technical program, by loss late after the high temperature granulating only in 5%, guaranteed to the full extent the product effect, secondly this bacterium is aerobic bacteria, can reduce oxygen density in the animal intestinal, thereby the Growth and Reproduction that suppresses pernicious bacteria also has the functions such as activity of repairing intestinal mucosa, promoting the various digestive ferments of intestines and stomach; The employed bacillus that condenses can lactic acid producing can produce gemma again in the technical program, have acidproof, heat-resisting, salt tolerant, cultivate and the characteristics such as preservation easily, and the advantage that combines Bacillus acidi lactici and bacillus subtilis.
The technical program is that acidulant has adopted citric acid, lactic acid and coated phosphoric acid to be used, wherein, coated phosphoric acid can reach the effect of rapid reduction pig stomach inner pH value, can keep again good shock-absorbing capacity and biological property, citric acid, lactic acid and coated phosphoric acid are used with adopting pure organic acid combination and compare simultaneously, can also reduce the interpolation cost.
Among the present invention Chinese traditional herbs and functional polysaccharide, probiotics, acidulant, plants essential oil etc. are carried out combination collocation, the cooperative effect performance of Chinese traditional herbs and other component is extremely maximum, energy is substitute antibiotics fully, reduce and perplex for a long time the problems such as animal husbandry antibiotic resistance, medicine for a long time is residual, Use out of range, play simultaneously effects such as improving breeding environment, reduction feed safety hidden danger, raising culture benefit.
The specific embodiment
Now in conjunction with specific embodiments, describe the present invention in detail.
Embodiment one
A kind of alternative antibiotic pig feed additive, component and parts by weight thereof in its prescription are: 1 part of Divine Comedy, 1 part of Radix Angelicae Sinensis, 1 part of the Radix Astragali, 1 part of hawthorn, 1 part of Radix Codonopsis; 10 parts of citric acids, 10 parts of lactic acid, 20 parts of coated phosphoric acid, 5 parts of probioticses, 5 parts of yeast cell wall polysaccharide, 5 parts of 15 parts of composite plant essential oils and white carbons.
Wherein, probiotics comprises following composition: 1.0 * 10 9The bafillus natto of CFU/g, 1.0 * 10 9The CFU/g bacillus that condenses, the weight ratio of bafillus natto and the bacillus that condenses is 1:1; The composite plant essential oil comprises following composition and mass percent: Thymol: 1%, and geraniol: 0.5%, bergamot oil: 9%, surplus is vegetable oil and absorbent-type carrier; Coated phosphoric acid is the mixture of list/bi-tristearin and phosphoric acid, and the weight ratio of described list/bi-tristearin and described phosphoric acid is 0.5:1; Carrier is white carbon.
A kind of preparation method of alternative antibiotic pig feed additive comprises the steps, wherein umber is parts by weight:
1), 20 purpose Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis are carried out respectively 300 order ultramicro grinding broken wall treatment, sporoderm-broken rate is more than 80%, 1 part of 1 part of 1 part of 1 part of 1 part of Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis taking by weighing after the broken wall treatment fully mix 140 seconds again, and it is for subsequent use to make composite Chinese herbal;
2) list of a certain amount of solid sheet/bi-tristearin is added in the stainless steel cauldron, insulation after being heated to 82 ℃, again 85% food-class liquid phosphoric acid is added in the reactor, keep reaction temperature at 82 ℃, speed of agitator 480 r/min, the reaction time is 110min, make coated phosphoric acid, to be coated with phosphoric acid after question response is complete and place the heatproof Plastic Drum interior for subsequent use, wherein, the weight ratio of list/bi-tristearin and described phosphoric acid is 0.5:1;
3) take by weighing 10 parts of citric acids, 10 parts of lactic acid, 1 part of 20 parts of coated phosphoric acid and white carbon, use double helix cone-type mixer mixing 10min, put into baking car pallet after fully mixing, change in the heated-air circulation oven and dry, bake out temperature is 46 ℃, time 5.5h, adopt again coated phosphoric acid, lactic acid, citric acid and the carrier mixture that broken vibrations sieving machine will be dried to pulverize, it is for subsequent use to make compound acidulant after sieving, and wherein, the granularity after coated phosphoric acid, lactic acid, citric acid and carrier mixture are pulverized is greater than 80 orders;
4) with 2.5 part 1.0 * 10 9The bafillus natto of CFU/g, 2.5 part 1.0 * 10 9CFU/g condense 5 parts of bacillus, yeast cell wall polysaccharide, 1 part of 1 part of composite plant essential oil and white carbon stir fully that to make functional raw material behind the mixed 170S for subsequent use;
5) composite Chinese herbal, compound acidulant, functional raw material and the 3 parts of white carbons that make are placed in the three-dimensional motion mixer mix, incorporation time is that 200S namely makes alternative antibiotic pig feed additive.
The alternative antibiotic pig feed additive that makes can be added into swine feed and use by the amount of the interpolation of 3kg/T.
In order to prove the performance of present embodiment, done following test:
Test feed addictive: the pig that the embodiment of the invention 1 provides substitute antibiotics additive;
Test site: Guangdong Jiangmen scale pig farm (2000 of basic sows);
Experimental animal: select 160 of the outer three way cross piglets (26-28 age in days) of healthy neat uniformly DLY;
Test method: the principle close with body weight, that sex ratio is close is divided into 2 processing (control group and test group) at random, and each processes 80, minute 5 repetitions in every processing, and each repeats 16 piglets.Control group uses the general goods feed (to contain antibiotic colistine sulfate 20mg/kg, kitasamycin 50 mg/kg, Bacitracin Zinc 100 mg/kg, meet national drug and add standard), test group does not contain antibiotic, for better checking product effect of the present invention, only add 0.3% pig substitute antibiotics additive.Whole feeding process is fed by same poultry raiser, Manual material feeding, free choice feeding, freely drinks water, and each group all gives identical feeding and management and environmental condition;
Test period: raise in advance 7 days phases, since formal test in the 8th day, 30 days by a definite date;
Weighing method: the 8:00-10:00 in morning that formal test begins the same day and finishes the same day carries out empty stomach to all pigs and weighs, and the front activity 10 minutes of first pig fool of weighing is discharged feces until it and to be carried out that individuality is weighed, record after urinating;
Average weight and average daily gain method of testing: experimental period, weigh by head when beginning and finishing, and calculates every pig
Weightening finish, daily gain are calculated the average daily ingestion amount, average daily gain of two processed group, average feedstuff-meat ratio again;
Result of the test: feeding experiment the results are shown in Table shown in 1.
Table 1 adds 0.3% impact on weaned piglets
Process Control group Test group
Repeat number 5 5
Process a number 80 80
The average age in days of formal test 33-35 33-35
The test fate 30 30
Initial weight (kg) 10.18±1.39 10.09±1.54
End heavy (kg) 25.93±2.41 26.32±2.25
Average daily gain 525±78 541±65
Average daily ingestion amount 841±76 843±83
Feedstuff-meat ratio 1.64±0.06 1.61±0.05
Full phase diarrhea rate (%) 5.98±2.86 4.63±1.53
As can be seen from Table 1: initial weight there is no significant difference (P〉0.05) between two groups; Daily ration adds 0.3% pig with behind the substitute antibiotics additive, and the end is heavy improves 2% than control group, without significant difference (P〉0.05); Average daily gain improves 3.05% than control group, without significant difference (P〉0.05); Daily ingestion amount improves 1.39% than control group, without significant difference (P〉0.05); Feedstuff-meat ratio is low by 1.6% than control group, without significant difference (P〉0.05); Diarrhea rate reduces by 22.58% than control group, without significant difference (P〉0.05).The pig that the result of the test explanation embodiment of the invention 1 provides can substitute fully with substitute antibiotics additive (0.3% adds) and have antibiotic combinations now, and the integral production performance all slightly is improved, but without significant difference.
Embodiment two
A kind of alternative antibiotic pig feed additive, component and parts by weight thereof in its prescription are:
2 parts of Divine Comedy, 2 parts of Radix Angelicae Sinensis, 2 parts of the Radixs Astragali, 3 parts of hawthorn, 1 part of Radix Codonopsis; 15 parts of citric acids, 15 parts of lactic acid, 25 parts of coated phosphoric acid, 8 part 1.0 * 10 9The bafillus natto of CFU/g, 0.8 part 1.0 * 10 8The bacillus that condenses of CFU/g, 8 parts of yeast cell wall polysaccharide, 2 parts of composite plant essential oils, and 9 parts of medical stones.
Wherein, the composite plant essential oil comprises following composition and mass percent: Thymol: 2%, and geraniol: 1%, bergamot oil: 10%, surplus is vegetable oil and absorbent-type carrier; Coated phosphoric acid is the mixture of list/bi-tristearin and phosphoric acid, and the weight ratio of described list/bi-tristearin and described phosphoric acid is 0.65:1.
A kind of preparation method of alternative antibiotic pig feed additive comprises the steps, wherein umber is parts by weight:
1), 60 purpose Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis are carried out respectively 300 order ultramicro grinding broken wall treatment, sporoderm-broken rate is more than 80%, the Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, the Radix Codonopsis that take by weighing in proportion after the broken wall treatment fully mix 150 seconds again, and it is for subsequent use to make composite Chinese herbal;
2) list of a certain amount of solid sheet/bi-tristearin is added in the stainless steel cauldron, insulation after being heated to 85 ℃, to in proportion 85% food-class liquid phosphoric acid be added in the reactor again, keep reaction temperature at 85 ℃, speed of agitator 500r/min, reaction time is 120min, makes coated phosphoric acid, will be coated with phosphoric acid after question response is complete and place in the heatproof Plastic Drum for subsequent use;
3) take by weighing in proportion citric acid, lactic acid and coated phosphoric acid, take by weighing simultaneously 2 parts of medical stones, use double helix cone-type mixer mixing 10min, put into baking car pallet after fully mixing, change in the heated-air circulation oven and dry, bake out temperature is 50 ℃, time 6h, adopt again coated phosphoric acid, lactic acid, citric acid and the carrier mixture that broken vibrations sieving machine will be dried to pulverize, it is for subsequent use to make compound acidulant after sieving, wherein, the granularity after coated phosphoric acid, lactic acid, citric acid and carrier mixture are pulverized is greater than 80 orders;
4) with 8 part 1.0 * 10 9The bafillus natto of CFU/g and 0.8 part 1.0 * 10 8The bacillus that condenses of CFU/g, 8 parts of yeast cell wall polysaccharide, it is for subsequent use to make functional raw material behind 2 parts of mixed 180S of abundant stirring of 2 parts of composite plant essential oils and medical stone;
5) composite Chinese herbal, compound acidulant, functional raw material and the 5 parts of carriers that make are placed in the three-dimensional motion mixer mix, incorporation time is that 210S namely makes alternative antibiotic pig feed additive.
The alternative antibiotic pig feed additive that makes can be added into swine feed and use by the amount of the interpolation of 4kg/T.
In order to prove the performance of present embodiment, done following test:
Test feed addictive: the pig that the embodiment of the invention 2 provides substitute antibiotics additive;
Test site: scale pig farm, Ganzhou (3000 of basic sows);
Experimental animal: select 120 of healthy neat uniformly DLY three way cross piglets (29 age in days).The weak malicious seedling vaccine of the pseudo-mad dog gE gene delection of collunarium immunity Pfizer Inc. during piglet 3 age in days; The swine fever cell freeze dried vaccine of musculi colli injection Pfizer Inc. during piglet 14 age in days;
Test method: 120 three way cross weanling pigs are divided into 2 processing (control group and test group) at random, and 5 repetitions are established in every processing, 12 piglets of every repetition.Control group uses general goods feed (contain antibiotic colistine sulfate 20mg/kg, flavomycoin 20mg/kg, meet national drug and add standard), and test group does not contain antibiotic, adds 0.4% pig substitute antibiotics additive.The raising column home is sealing, slatted floor formula pig house, the pig free choice feeding, and automatic drinking bowl supplies water, and pig is fed according to routine raising operational procedure with the grouping feed after entering the hurdle;
Test period: tested 30 days;
Weigh and blood-sampling method: the 8:00-10:00 in morning that formal test begins the same day and finishes the same day carries out empty stomach to all pigs and weighs, and the front activity 10 minutes of first pig fool of weighing is discharged feces until it and to be carried out that individuality is weighed, record after urinating; Piglet the 28th, 58 ages in days (testing the 0th, 30 day), repeat to get totally 20 of the blood sampling 3ml(every processing of lying on the back of 4 piglet vena cava anteriors from every), place 30 min in 37 ℃, then low-speed centrifugal is with separation of serum, place-20 ℃ of Refrigerator stores, wait until the mensuration of blood parameters.Morning, noon and afternoon every day are observed piglet ight soil situation, record each and repeat piglet total diarrhoea every day number, calculate diarrhea frequency, and then calculate each and process full phase diarrhea rate.
Average weight and average daily gain method of testing: experimental period, weigh by head when beginning and finishing, and calculates every pig weightening finish, daily gain, calculates average daily ingestion amount, average daily gain, the average feedstuff-meat ratio of two processed group again;
Blood parameters detection method: the activity that adopts alkali phosphorus enzyme, superoxide dismutase in the kit measurement serum of buying on the market; Adopt the kit of buying on thunder Du's RT-9600 semi-automatic biochemical analyzer and the market to detect endotoxic content in the serum; Use German Europe despot XL-200 import automatic clinical chemistry analyzer to measure respectively the content of glutamic-oxalacetic transaminease in the serum, glutamic-pyruvic transaminase, urea nitrogen, creatinine, T-CHOL, triglycerides, blood sugar, total protein, albumin and blood ammonia;
Result of the test: the production performance result of the test sees Table shown in 2, and physiochemical indice the results are shown in Table shown in 3.
Table 2 adds 0.4% impact on weaned piglets
Process Control group Test group
Repeat number 5 5
Process a number 60 60
The average age in days of formal test 29 29
The test fate 30 30
Initial weight (kg) 7.31±1.19 7.24±1.23
End heavy (kg) 20.57±2.15 21.28±2.34
Average daily gain 442±68 468±57
Average daily ingestion amount 720±96 741±83
Feedstuff-meat ratio 1.63±0.11 1.58±0.08
Full phase diarrhea rate (%) 6.35±1.94 4.24±1.03
As can be seen from Table 2: initial weight there is no significant difference (P〉0.05) between two groups; After daily ration added 0.4% pig usefulness substitute antibiotics additive, the end is heavy improved 3.45% than control group, significant difference (P＜0.05); Daily gain improves 5.88% than control group, significant difference (P＜0.05); Daily ingestion amount improves 2.92% than control group, without significant difference (P〉0.05); Feedstuff-meat ratio is low by 2.80% than control group, significant difference (P＜0.05); Full phase diarrhea rate reduces by 33.23% than control group, and difference is (P＜0.01) extremely significantly.The pig that the result of the test explanation embodiment of the invention 1 provides not only can substitute existing antibiotic combinations fully with substitute antibiotics additive (0.4% adds), and the integral production performance is improved, and feedstuff-meat ratio reduces situation, daily gain improves and the equal difference of full phase diarrhea rate reduction situation is remarkable.
Table 3 adds 0.4% impact on the piglet blood index
As can be seen from Table 3, control group and each index of test group blood test the 0th day with different variations was all arranged on the 30th day, control group and test group the 30th day were compared with the 0th day, and glutamic-oxalacetic transaminease improves respectively 10.45% and 27.85%, and difference is (P＜0.01) extremely significantly; Glutamic-pyruvic transaminase improves respectively 86.99% and 99.53%, and difference is (P＜0.01) extremely significantly; Alkali phosphorus enzyme reduces respectively 15.27% and 16.35%, and difference is (P＜0.01) extremely significantly; Superoxide dismutase improves respectively 3.61% and 12.42%, significant difference (P＜0.05); Total protein improves respectively 32.12% and 54.25%, and difference is (P＜0.01) extremely significantly; Albumin improves respectively 26.46% and 31.49%, and difference is (P＜0.01) extremely significantly; Endotoxin improves respectively 67.95% and 34.55%, and difference is (P＜0.01) extremely significantly; Urea nitrogen improves respectively 16.48% and 24.69%, and difference is (P＜0.01) extremely significantly; Creatinine improves respectively 25.28% and 34.43%, and difference is (P＜0.01) extremely significantly; T-CHOL improves respectively 47.32% and 56.94%, and difference is (P＜0.01) extremely significantly; Triglycerides improves respectively 25.49% and 21.05%, and difference is (P＜0.01) extremely significantly; Blood sugar improves respectively 7.91% and 28.80%, significant difference (P＜0.05); Blood ammonia reduces respectively 16.89% and 22.54%, significant difference (P＜0.05);
Test group the 30th day was compared with control group on the 30th day, and glutamic-oxalacetic transaminease improves 11.32%, and difference is (P＜0.01) extremely significantly; Glutamic-pyruvic transaminase improves 5.86%, significant difference (P＜0.05); Alkali phosphorus enzyme reduces by 1.72%, without significant difference (P＞0.05); Superoxide dismutase improves 11.00%, and difference is (P＜0.01) extremely significantly; Total protein improves 13.76%, significant difference (P＜0.05); Albumin improves 5.29%, significant difference (P＜0.05); Endotoxin reduces by 43.51%, and difference is (P＜0.01) extremely significantly; Urea nitrogen reduces by 2.57%, without significant difference (P＞0.05); Creatinine improves 5.10%, without significant difference (P＞0.05); T-CHOL improves 8.61%, significant difference (P＜0.05); Triglycerides improves 7.81%, significant difference (P＜0.05); Blood sugar improves 24.08%, and difference is (P＜0.01) extremely significantly; Blood ammonia reduces by 3.90%, without significant difference (P＞0.05);
Above test data statistics shows, compare with the antibiotic processing of interpolation, but add 0.4% pig and significantly improve piglet blood-sugar content and superoxide dismutase activity with the substitute antibiotics additive utmost point, the blood-sugar content increase shows that intestine of young pigs strengthens to some extent to the absorption of nutriment, and superoxide dismutase activity raises and shows that piglet body antiradical activities, anti-stress ability strengthen to some extent; Control group piglet blood endotoxin content exceeds 77.03% than test group, difference is extremely remarkable, illustrate and use antibiotic may cause the interior flora of intestine of young pigs unbalance, enteron aisle is got muddled, make the not high pathogenic bacteria of content own produce the resistance materials such as a large amount of endotoxins, cause other infection, as diarrhoea etc.
Embodiment three
A kind of alternative antibiotic pig feed additive, component and parts by weight thereof in its prescription are:
5 parts of Divine Comedy, 5 parts of Radix Angelicae Sinensis, 5 parts of the Radixs Astragali, 5 parts of hawthorn, 5 parts of Radix Codonopsis; 20 parts of citric acids, 20 parts of lactic acid, 30 parts of coated phosphoric acid, 6 parts of probioticses, 10 parts of yeast cell wall polysaccharide, 10 parts of 5 parts of composite plant essential oils and maize cob meals.
Wherein, probiotics comprises following composition: 1.0 * 10 8The bafillus natto of CFU/g, 1.0 * 10 8The CFU/g bacillus that condenses, the weight ratio of bafillus natto and the bacillus that condenses is 2:1; The composite plant essential oil comprises following composition and mass percent: Thymol: 2%, and geraniol: 1%, bergamot oil: 11%, surplus is vegetable oil and absorbent-type carrier; Coated phosphoric acid is the mixture of list/bi-tristearin and phosphoric acid, and the weight ratio of described list/bi-tristearin and described phosphoric acid is 0.6:1.
A kind of preparation method of alternative antibiotic pig feed additive comprises the steps, wherein umber is parts by weight:
1), 80 purpose Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis are carried out respectively 300 order ultramicro grinding broken wall treatment, sporoderm-broken rate is more than 80%, the Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn and the Radix Codonopsis that take by weighing in proportion after the broken wall treatment fully mix 160 seconds again, and it is for subsequent use to make composite Chinese herbal;
2) list of a certain amount of solid sheet/bi-tristearin is added in the stainless steel cauldron, insulation after being heated to 87 ℃, to take by weighing in proportion 85% food-class liquid phosphoric acid adds in the reactor again, keep reaction temperature at 87 ℃, speed of agitator 520r/min, reaction time is 130min, makes coated phosphoric acid, and places in the heatproof Plastic Drum for subsequent use;
3) take by weighing 20 parts of citric acids, 20 parts of lactic acid, 2 parts of 30 parts of coated phosphoric acid and maize cob meals, use double helix cone-type mixer mixing 11min, put into baking car pallet after fully mixing, change in the heated-air circulation oven and dry, bake out temperature is 50 ℃, time 6.5h, adopt again coated phosphoric acid, lactic acid, citric acid and the carrier mixture that broken vibrations sieving machine will be dried to pulverize, it is for subsequent use to make compound acidulant after sieving, and wherein, the granularity after coated phosphoric acid, lactic acid, citric acid and carrier mixture are pulverized is greater than 80 orders;
4) with 1.0 * 10 84 parts of the bafillus nattos of CFU/g, 1.0 * 10 8Condense 2 parts of bacillus of CFU/g, 10 parts of yeast cell wall polysaccharide, 2 parts of 5 parts of composite plant essential oils and maize cob meals fully stir mixed 190S, and it is for subsequent use to make functional raw material;
5) composite Chinese herbal, compound acidulant, functional raw material and the 6 parts of maize cob meals that make are placed in the three-dimensional motion mixer mix, incorporation time is that 220S namely makes alternative antibiotic pig feed additive.
This alternative antibiotic pig feed additive is added into the swine feed use by the amount of the interpolation of 4kg/T.
There are basic sow 400 remainders on Guangxi province Hechi City pig farm, rear to the 60kg stage in the wean of piglet 28-33 age in days, long-term use product of the present invention and be added in the swine feed with 4kg/T use, this obviously raising of reaction swinery health status, effect stability, epidemic situation control are normally, diarrhea rate reduces about 8%, 60 age in days child care piglets are delivered for sale and all focus on 21.16kg the whole years, use antibiotic pig farm to compare with local great majority and on average exceed 1-2kg.
Embodiment four
A kind of alternative antibiotic pig feed additive, component and parts by weight thereof in its prescription are:
2 parts of Divine Comedy, 4 parts of Radix Angelicae Sinensis, 3 parts of the Radixs Astragali, 2 parts of hawthorn, 2 parts of Radix Codonopsis; 13 parts of citric acids, 16 parts of lactic acid, 25 parts of coated phosphoric acid, 10 parts of probioticses, 6 parts of yeast cell wall polysaccharide, 8 parts of 4 parts of composite plant essential oils and maltodextrins.
Wherein, probiotics comprises following composition: 1.0 * 10 8The bafillus natto of CFU/g, 1.0 * 10 8～1.0 * 10 9The CFU/g bacillus that condenses, the weight ratio of bafillus natto and the bacillus that condenses is 4:1;
Wherein, the composite plant essential oil comprises following composition and mass percent: Thymol: 1.5%, and geraniol: 0.8%, bergamot oil: 10%, surplus is vegetable oil and absorbent-type carrier.
Coated phosphoric acid is the mixture of list/bi-tristearin and phosphoric acid, and the weight ratio of described list/bi-tristearin and described phosphoric acid is 0.6:1.
A kind of preparation method of alternative antibiotic pig feed additive comprises the steps, wherein umber is parts by weight:
1), 60 purpose Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis are carried out respectively 300 order ultramicro grinding broken wall treatment, sporoderm-broken rate is more than 80%, the Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, the Radix Codonopsis that take by weighing in proportion after the broken wall treatment fully mix 150 seconds again, and it is for subsequent use to make composite Chinese herbal;
2) list of a certain amount of solid sheet/bi-tristearin is added in the stainless steel cauldron, insulation after being heated to 85 ℃, to in proportion 85% food-class liquid phosphoric acid be added in the reactor again, keep reaction temperature at 85 ℃, speed of agitator 500r/min, reaction time is 120min, makes coated phosphoric acid, and places in the heatproof Plastic Drum for subsequent use;
3) take by weighing 13 parts of citric acids, 16 parts of lactic acid, 2 parts of 25 parts of coated phosphoric acid and maltodextrins, use double helix cone-type mixer mixing 10min, put into baking car pallet after fully mixing, change in the heated-air circulation oven and dry, bake out temperature is 48 ℃, time 6h adopts coated phosphoric acid, lactic acid, citric acid and the carrier mixture that broken vibrations sieving machine will be dried to pulverize again, and it is for subsequent use to make compound acidulant after sieving;
4) with 1.0 * 10 88 parts of the bafillus nattos of CFU/g, 1.0 * 10 8～1.0 * 10 9Condense 2 parts of bacillus of CFU/g, 6 parts of yeast cell wall polysaccharide, 1 part of 4 parts of composite plant essential oils and maltodextrin stir fully that to make functional raw material behind the mixed 170-190S for subsequent use;
5) composite Chinese herbal, compound acidulant, functional raw material and the 5 parts of carriers that make are placed in the three-dimensional motion mixer mix, incorporation time is that 210S namely makes alternative antibiotic pig feed additive.
During the application of this alternative antibiotic pig feed additive, can be added into swine feed by the amount of the interpolation of 3.3kg/T and use.
There are basic sow 600 remainders on the pig farm on Yiyang City high mountain, Hunan Province, long-term use products substitution Antibiotic Additive of the present invention, its using method is, use pig to substitute whole antibiotic with substitute antibiotics additive 0.4% in piglet 30 ages in days wean to the 63 age in days child care stage of delivering for sale, diarrhea rate hangs down about 5%, the child care piglet is delivered for sale and all focuses on 21.35kg, and local other use antibiotic pig farm on average to exceed 1.5-2.5kg; Use to piglet 100 ages in days with substitute antibiotics additive substitute antibiotics with 0.3% pig after the child care piglet is delivered for sale, counterpoise reaches about 50-52kg again, and epidemic situation control is normal, the pig health condition is better.
Embodiment five
A kind of alternative antibiotic pig feed additive, component and parts by weight thereof in its prescription are:
4 parts of Divine Comedy, 4 parts of Radix Angelicae Sinensis, 4 parts of the Radixs Astragali, 4 parts of hawthorn, 4 parts of Radix Codonopsis; 15 parts of citric acids, 15 parts of lactic acid, 25 parts of coated phosphoric acid, 6 parts of probioticses, 8 parts of yeast cell wall polysaccharide, 7 parts of 3 parts of composite plant essential oils and maize cob meals.
Wherein, probiotics comprises following composition: 1.0 * 10 8The bafillus natto of CFU/g, 1.0 * 10 8The CFU/g bacillus that condenses, the weight ratio of bafillus natto and the bacillus that condenses is 2:1; The composite plant essential oil comprises following composition and mass percent: Thymol: 1.6%, and geraniol: 0.7%, bergamot oil: 10%, surplus is vegetable oil and absorbent-type carrier; Coated phosphoric acid is the mixture of list/bi-tristearin and phosphoric acid, and the weight ratio of described list/bi-tristearin and described phosphoric acid is 0.6:1.
A kind of preparation method of alternative antibiotic pig feed additive comprises the steps, wherein umber is parts by weight:
1), 80 purpose Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis are carried out respectively 300 order ultramicro grinding broken wall treatment, sporoderm-broken rate is more than 80%, the Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn and the Radix Codonopsis that take by weighing in proportion after the broken wall treatment fully mix 160 seconds again, and it is for subsequent use to make composite Chinese herbal;
2) list of a certain amount of solid sheet/bi-tristearin is added in the stainless steel cauldron, insulation after being heated to 86 ℃, to take by weighing in proportion 85% food-class liquid phosphoric acid adds in the reactor again, keep reaction temperature at 86 ℃, speed of agitator 520r/min, reaction time is 130min, makes coated phosphoric acid, and places in the heatproof Plastic Drum for subsequent use;
3) take by weighing 15 parts of citric acids, 15 parts of lactic acid, 2 parts of 25 parts of coated phosphoric acid and maize cob meals, use double helix cone-type mixer mixing 11min, put into baking car pallet after fully mixing, change in the heated-air circulation oven and dry, bake out temperature is 50 ℃, time 6.5h, adopt again coated phosphoric acid, lactic acid, citric acid and the carrier mixture that broken vibrations sieving machine will be dried to pulverize, it is for subsequent use to make compound acidulant after sieving, and wherein, the granularity after coated phosphoric acid, lactic acid, citric acid and carrier mixture are pulverized is greater than 80 orders;
4) with 1.0 * 10 84 parts of the bafillus nattos of CFU/g, 1.0 * 10 8Condense 2 parts of bacillus of CFU/g, 8 parts of yeast cell wall polysaccharide, 2 parts of 3 parts of composite plant essential oils and maize cob meals fully stir mixed 190S, and it is for subsequent use to make functional raw material;
5) composite Chinese herbal, compound acidulant, functional raw material and the 3 parts of maize cob meals that make are placed in the three-dimensional motion mixer mix, incorporation time is that 220S namely makes alternative antibiotic pig feed additive.
This alternative antibiotic pig feed additive is added into the swine feed use by the amount of the interpolation of 4kg/T.
In order to prove the performance of present embodiment, done following test:
Test feed addictive: blank group; The pig that the embodiment of the invention 1 provides substitute antibiotics additive; Contrast groups 1: additive comprises following weight portion, by the Radix Astragali 200, spina date seed 200, rhizome of cyrtomium 150, wilsonii 100, the root of herbaceous peony 100, Fructus Hordei Germinatus 100, Divine Comedy 100 and dried orange peel 50, is ground into respectively powder, then mixes to get final product, and use amount is for using 4kg/T);
Test site: Zhanjiang scale pig farm (2000 of basic sows);
Experimental animal: select 180 of healthy neat uniformly two-way cross (long/large) piggys (60 age in days);
Test method: be divided at random 3 processing (blank group, contrast patent group, test group) so that body weight is close as principle, each processes 60, minute 5 repetitions in every processing, and each repeats 12 piglets.The blank group is used general goods feed (contain antibiotic colistine sulfate 20mg/kg and phenalgin arsenic acid 100mg/kg, meet national drug and add standard); Contrast groups 1 does not contain antibiotic, for better verifying product effect of the present invention, adds pure Chinese medicine pig's feed additive with 1% content in the contrast groups 1; Test group does not contain antibiotic, adds product pig substitute antibiotics additive of the present invention with 0.4% content.Whole feeding process is fattened house at same and is fed by same poultry raiser, guarantees pig energy free choice feeding, freely drinks water, and each group all gives identical feeding and management and environmental condition;
Test period: 30 days;
Weighing method: the 7:00 to 10:00 in morning on the same day of on-test and end carries out, and the front pig of weighing needs on an empty stomach, before electronic scale limit hurdle is being caught up with on whole hurdle first, again by head catch up with into the limit hurdle weigh, record;
Test data: record diarrhea rate every day and then calculate full phase diarrhea rate, initial weight, end weight, average daily gain, on average daily ingestion amount, feedstuff-meat ratio;
Result of the test: feeding experiment the results are shown in Table shown in 8.
Table 4 adds 0.4% the present invention and control group 1 adds 1% impact on the piggy production performance
Process The blank group Contrast groups 1 Test group
Repeat number 5 5 5
Process a number 60 60 60
Initial weight (kg) 20.38±1.79 20.65±1.67 20.29±1.71
End heavy (kg) 40.03±3.23 40.86±2.74 41.11±2.99
Average daily gain 655±89 667±75 694±94
Average daily ingestion amount 1269±142 1280±156 1305±169
Feedstuff-meat ratio 1.94±0.18 1.92±0.17 1.88±0.15
Full phase diarrhea rate (%) 7.24±1.88 5.65±1.51 4.49±0.72
As can be seen from Table 4: initial weight there is no significant difference (P〉0.05) between three groups; The pure Chinese herbal medicine of contrast groups 1() improves 1.83% than the average daily gain of blank group, without significant difference (P〉0.05), average daily ingestion amount improves 0.87%, without significant difference (P〉0.05), feedstuff-meat ratio reduces by 0.95%, without significant difference (P〉0.05), diarrhea rate reduces by 21.96, significant difference (P＜0.05); Test group (product of the present invention) improves 5.95% than the average daily gain of blank group, (P＜0.05), average daily ingestion amount improves 2.84%, without significant difference (P〉0.05), feedstuff-meat ratio reduces by 2.94%, significant difference (P＜0.05), diarrhea rate reduces by 37.98%, and difference is (P＜0.01) extremely significantly; Test group (product of the present invention) is than the pure Chinese herbal medicine of contrast groups 1() average daily gain raising 4.05%, significant difference (P＜0.05), average daily ingestion amount improves 1.95%, without significant difference (P〉0.05), feedstuff-meat ratio reduces by 2.01%, without significant difference (P〉0.05), diarrhea rate reduces by 20.53%, and difference is (P＜0.01) extremely significantly.The pig that the result of the test explanation embodiment of the invention 1 provides can substitute existing antibiotic combinations fully with substitute antibiotics additive (0.4% adds), and it number is embodiment 1 described product (1% adds) in 200410084602.6 that the integral production performance all is better than Chinese patent application, it is extremely remarkable to find expression in diarrhea rate reduction difference, average daily gain improves significant difference, but average daily ingestion amount improves and feedstuff-meat ratio reduction difference is not remarkable.
Embodiment six
A kind of alternative antibiotic pig feed additive, component and parts by weight thereof in its prescription are:
5 parts of Divine Comedy, 5 parts of Radix Angelicae Sinensis, 5 parts of the Radixs Astragali, 5 parts of hawthorn, 5 parts of Radix Codonopsis; 17 parts of citric acids, 17 parts of lactic acid, 23 parts of coated phosphoric acid, 8 parts of probioticses, 9 parts of yeast cell wall polysaccharide, 10 parts of 4 parts of composite plant essential oils and maize cob meals.
Wherein, probiotics comprises following composition: 1.0 * 10 8The bafillus natto of CFU/g, 1.0 * 10 8The CFU/g bacillus that condenses, the weight ratio of bafillus natto and the bacillus that condenses is 5:1; The composite plant essential oil comprises following composition and mass percent: Thymol: 1.2%, and geraniol: 0.8%, bergamot oil: 9%, surplus is vegetable oil and absorbent-type carrier; Coated phosphoric acid is the mixture of list/bi-tristearin and phosphoric acid, and the weight ratio of described list/bi-tristearin and described phosphoric acid is 0.6:1.
A kind of preparation method of alternative antibiotic pig feed additive comprises the steps, wherein umber is parts by weight:
1), 80 purpose Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis are carried out respectively 300 order ultramicro grinding broken wall treatment, sporoderm-broken rate is more than 80%, the Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn and the Radix Codonopsis that take by weighing in proportion after the broken wall treatment fully mix 160 seconds again, and it is for subsequent use to make composite Chinese herbal;
2) list of a certain amount of solid sheet/bi-tristearin is added in the stainless steel cauldron, insulation after being heated to 86 ℃, to take by weighing in proportion 85% food-class liquid phosphoric acid adds in the reactor again, keep reaction temperature at 86 ℃, speed of agitator 520r/min, reaction time is 130min, makes coated phosphoric acid, and places in the heatproof Plastic Drum for subsequent use;
3) take by weighing 17 parts of citric acids, 17 parts of lactic acid, 3 parts of 23 parts of coated phosphoric acid and maize cob meals, use double helix cone-type mixer mixing 11min, put into baking car pallet after fully mixing, change in the heated-air circulation oven and dry, bake out temperature is 50 ℃, time 6.5h, adopt again coated phosphoric acid, lactic acid, citric acid and the carrier mixture that broken vibrations sieving machine will be dried to pulverize, it is for subsequent use to make compound acidulant after sieving, and wherein, the granularity after coated phosphoric acid, lactic acid, citric acid and carrier mixture are pulverized is greater than 80 orders;
4) with 1.0 * 10 85 parts of the bafillus nattos of CFU/g, 1.0 * 10 8Condense 1 part of bacillus of CFU/g, 9 parts of yeast cell wall polysaccharide, 2 parts of 4 parts of composite plant essential oils and maize cob meals fully stir mixed 190S, and it is for subsequent use to make functional raw material;
5) composite Chinese herbal, compound acidulant, functional raw material and the 4 parts of maize cob meals that make are placed in the three-dimensional motion mixer mix, incorporation time is that 220S namely makes alternative antibiotic pig feed additive.
This alternative antibiotic pig feed additive is added into the swine feed use by the amount of the interpolation of 3kg/T.
In order to prove the performance of present embodiment, done following test:
Test feed addictive: the pig that the embodiment of the invention 1 provides substitute antibiotics additive; Contrast groups 2: lactic acid bacillus 1.0 * 10 9CFU/g, bacillus subtilis: 1.0 * 10 9CFU/g; Manna oligosacchride: 0.3 % by weight; Lactic acid: 10 % by weight, citric acid: 5 % by weight; Surplus is white carbon, takes by weighing first lactic acid and carries out carrier adsorption, is mixed with lactic acid bacillus, bacillus subtilis, manna oligosacchride, citric acid again, stirs abundant mixing, and the addition in mixed feed is 3kg/T.
Test site: Mianyang, Sichuan scale pig farm (3000 of basic sows);
Experimental animal: select 165 of three way cross (Du/long/large) piglets (28 age in days);
Test method:, sex ratio close with body weight is close to principle and is divided at random 3 processing (antibiotic control group, contrast 2 groups, test group of the present invention), and each processes 55, minute 5 repetitions in every processing, and each repeats 11 piglets.The antibiotic control group uses general goods feed (contain antibiotic colistine sulfate 20mg/kg and Nosiheptide 5mg/kg, meet national drug and add standard); Contrast patent group does not contain antibiotic, adds 0.3% embodiment, 2 described products; Test group does not contain antibiotic, adds 0.3% embodiment of the invention, 1 product.Raising whole process all carries out under the operating condition of standard gauge modelling pig farm; the nursery house neat and tidy; the omnidistance free choice feeding of pig, drinking-water; for better reduce environment on the impact of test (as sunshine length, humidity, stress wait factor); 15 repeating groups are the nursing cote of at random layout, and eye-catching nameplate is all arranged before every hurdle, except being undertaken by same poultry raiser whole process feeds; also send a track record person, prevent wrong to feed, mixed feed etc.
Test period: 33 days;
Weighing method: the 8:00 to 10:00 in morning on the same day of on-test and end carries out, and the front pig of weighing need be moved 5 minutes, and keeps on an empty stomach, again by head weigh, record;
Result of the test: feeding experiment the results are shown in Table shown in 5.
Table 5 adds 0.3% the present invention and adds 0.3% impact on weaned piglets with the contrast patent
Process The antibiotic control group Contrast groups 2 Test group of the present invention
Repeat number 5 5 5
Process a number 55 55 55
Initial weight (kg) 7.61±0.79 7.53±0.96 7.46±0.91
End heavy (kg) 22.72±1.57 23.24±1.74 23.76±1.87
Average daily gain 458±54 476±48 494±63
Average daily ingestion amount 768±86 792±74 799±107
Feedstuff-meat ratio 1.68±0.23 1.66±0.18 1.62±0.19
Full phase diarrhea rate (%) 7.97±2.17 6.36±1.38 5.49±0.86
As can be seen from Table 5: initial weight there is no significant difference (P〉0.05) between three groups; Contrast groups 2 improves 3.93% than the average daily gain of antibiotic control group, significant difference (P＜0.05), average daily ingestion amount improves 3.13%, without significant difference (P〉0.05), feedstuff-meat ratio reduces by 0.77%, without significant difference (P〉0.05), diarrhea rate reduces by 20.20, and difference is (P＜0.01) extremely significantly; Test group of the present invention improves 7.86% than the average daily gain of antibiotic control group, significant difference (P＜0.05), average daily ingestion amount improves 4.04%, significant difference (P＜0.05), feedstuff-meat ratio reduces by 3.55%, significant difference (P＜0.05), diarrhea rate reduces by 31.12%, and difference is (P＜0.01) extremely significantly; Test group of the present invention improves 3.78% than contrast groups 2 average daily gains, significant difference (P＜0.05), average daily ingestion amount improves 0.88%, without significant difference (P〉0.05), feedstuff-meat ratio reduces by 2.79%, significant difference (P＜0.05), diarrhea rate reduces by 13.68%, significant difference (P＜0.05).Result of the test illustrates that pig provided by the invention can substitute existing antibiotic combinations fully with substitute antibiotics additive (0.3% adds), and it number is embodiment 2 described products (0.3% adds) in 201110361980.4 that the integral production performance all is better than Chinese patent application, find expression in feedstuff-meat ratio reduction, average daily gain raising and diarrhea rate and reduce significant difference, but average daily ingestion amount raising difference is not remarkable.
More than technical scheme that the embodiment of the invention is provided be described in detail, used specific case herein principle and the embodiment of the embodiment of the invention are set forth, the explanation of above embodiment is only applicable to help to understand the principle of the embodiment of the invention; Simultaneously, for one of ordinary skill in the art, according to the embodiment of the invention, all will change on the specific embodiment and range of application, in sum, this description should not be construed as limitation of the present invention.

Claims (7)

1. an alternative antibiotic pig feed additive is characterized in that, component and parts by weight thereof in its prescription are:
Divine Comedy 1-5 part, Radix Angelicae Sinensis 1-5 part, Radix Astragali 1-5 part, hawthorn 1-5 part, Radix Codonopsis 1-5 part; Citric acid 10-20 part, lactic acid 10-20 part, coated phosphoric acid 20-30 part, probiotics 5-10 part, yeast cell wall polysaccharide 5-10 part, composite plant essential oil 1-5 part and carrier 5-10 part.
2. alternative antibiotic pig feed additive according to claim 1 is characterized in that probiotics comprises following composition: 1.0 * 10 8～1.0 * 10 9The bafillus natto of CFU/g, 1.0 * 10 8～1.0 * 10 9The CFU/g bacillus that condenses, bafillus natto is 1:1-10:1 with the weight ratio of the bacillus that condenses.
3. alternative antibiotic pig feed additive according to claim 1, it is characterized in that the composite plant essential oil comprises following composition and mass percent: Thymol: 1-2%, geraniol: 0.5-1%, bergamot oil: 9-11%, surplus is for being vegetable oil and absorbent-type carrier.
4. alternative antibiotic pig feed additive according to claim 1 is characterized in that, coated phosphoric acid is the mixture of list/bi-tristearin and phosphoric acid, and the weight ratio of described list/bi-tristearin and described phosphoric acid is 0.5-0.65:1.
5. alternative antibiotic pig feed additive according to claim 1 is characterized in that, described carrier is inorganic or organic carrier, and described organic carrier comprises maize cob meal or maltodextrin, and inorganic carrier comprises white carbon or medical stone.
6. the preparation method of each described alternative antibiotic pig feed additive of claim 1-5 is characterized in that, comprise the steps, wherein umber is parts by weight:
1), 20-80 purpose Divine Comedy, Radix Angelicae Sinensis, the Radix Astragali, hawthorn, Radix Codonopsis are carried out respectively 300 order ultramicro grinding broken wall treatment, sporoderm-broken rate is more than 80%, Divine Comedy 1-5 part, Radix Angelicae Sinensis 1-5 part, Radix Astragali 1-5 part, hawthorn 1-5 part, Radix Codonopsis 1-5 part of taking by weighing by weight after the broken wall treatment fully mix 140-160 second again, and it is for subsequent use to make composite Chinese herbal;
2) list of a certain amount of solid sheet/bi-tristearin is added in the stainless steel cauldron, insulation after being heated to 82-87 ℃, again 85% food-class liquid phosphoric acid is added in the stainless steel cauldron, keep reaction temperature at 82-87 ℃, speed of agitator 480-520r/min, the reaction time is 110-130min, make coated phosphoric acid, and place the heatproof Plastic Drum interior for subsequent use, wherein, the weight ratio of list/bi-tristearin and described phosphoric acid is 0.5-0.65:1;
3) take by weighing citric acid 10-20 part, lactic acid 10-20 part, coated phosphoric acid 20-30 part and carrier 1-2 part, use double helix cone-type mixer mixing 9-11min, put into baking car pallet after fully mixing, change in the heated-air circulation oven and dry, bake out temperature is 46-50 ℃, time 5.5-6.5h, the coated phosphoric acid that adopts again broken vibrations sieving machine to dry, lactic acid, citric acid and carrier mixture are pulverized, it is for subsequent use to make compound acidulant after sieving, wherein, and coated phosphoric acid, lactic acid, granularity after citric acid and carrier mixture are pulverized is greater than 80 orders;
4) with probiotics 5-10 part, yeast cell wall polysaccharide 5-10 part, composite plant essential oil 1-5 part and carrier 1-2 part stir fully that to make functional raw material behind the mixed 170-190S for subsequent use;
5) composite Chinese herbal, compound acidulant, functional raw material and the 3-6 part carrier that makes placed in the three-dimensional motion mixer mix, incorporation time is that 200-220S namely makes alternative antibiotic pig feed additive.
7. the application of each described alternative antibiotic pig feed additive of claim 1-6 is characterized in that, described alternative antibiotic pig feed additive is added into swine feed by the addition of 3-4kg/T uses.
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CN105767532A (en) * 2016-03-22 2016-07-20 江西天佳动物药业有限公司 Compound plant essential oil-type feed additive capable of replacing antibiotics
CN105941902A (en) * 2016-04-29 2016-09-21 湖南九鼎科技（集团）有限公司 Antibiotic-free feed for fattening-stage pigs and preparation method thereof
CN106343155A (en) * 2016-04-14 2017-01-25 安徽华瑞医药技术开发有限公司 Preparation method for functional feed additive
CN106359902A (en) * 2016-09-22 2017-02-01 广西大学 Traditional Chinese medicine feed additive capable of replacing antibiotics
CN106578386A (en) * 2016-12-20 2017-04-26 河南科技学院 Thymol new application
CN106889328A (en) * 2017-02-22 2017-06-27 湖南九鼎科技（集团）有限公司 Child care pig antibiotic-free feed and preparation method thereof
CN107156483A (en) * 2017-05-26 2017-09-15 深圳市金新农科技股份有限公司 It is a kind of to be used to improve the fodder compound, compound premix and functional feed of Intestinal Microflora of Weanling Piglets structure
CN107173546A (en) * 2017-06-12 2017-09-19 揭阳市润达肠衣有限公司 A kind of taste masking controlled release granule containing galic essential oil and its preparation method and application
CN107410729A (en) * 2017-05-18 2017-12-01 钱信美 A kind of pig feed additive
CN107691789A (en) * 2017-12-04 2018-02-16 广州农宝饲料有限公司 A kind of in-pig compound premixed feed and preparation method thereof
CN107950774A (en) * 2017-04-26 2018-04-24 潍坊柯能生物科技有限公司 A kind of feeding composite functional plants essential oil, preparation method and applications
CN108029854A (en) * 2017-12-10 2018-05-15 济南诺能生物工程有限公司 A kind of compound formulation of alternative feeding antibiotic
CN108419916A (en) * 2018-04-08 2018-08-21 俞小峰 A kind of preparation method of compound coating acidulant
CN108541820A (en) * 2018-05-19 2018-09-18 双胞胎（集团）股份有限公司 A kind of nursery-stage pig feed of antibiotic-free
CN108651709A (en) * 2018-03-26 2018-10-16 味氏（广东）生物科技股份有限公司 Antibacterial bacteriostatic growth accelerator and its preparation method and application
CN108740419A (en) * 2018-05-18 2018-11-06 雷山县方祥新科野猪林特种养殖专业合作社 A kind of boar feedstuff additive and preparation method thereof
CN109418540A (en) * 2017-08-21 2019-03-05 湖南现代资源生物科技有限公司 A kind of livestock and poultry growth promotion composition and preparation method thereof and purposes
CN110150494A (en) * 2019-06-25 2019-08-23 黑龙江大牧人牧业有限公司 A kind of piglet strong feed addictive of fruity rush and preparation method thereof
CN110301542A (en) * 2019-07-18 2019-10-08 张丽丽 A kind of feedstuff flavouring agent and its application
CN110771729A (en) * 2019-11-01 2020-02-11 广西壮族自治区林业科学研究院 Feed additive containing plant essential oil and preparation method thereof
CN110771730A (en) * 2019-11-01 2020-02-11 广西壮族自治区林业科学研究院 Livestock feed containing plant essential oil and preparation method thereof
CN110973357A (en) * 2019-12-31 2020-04-10 宜兴市天石饲料有限公司 Slow-release type compound acidifier and preparation method thereof
CN111631310A (en) * 2020-07-13 2020-09-08 四川大想生物科技有限公司 Anti-substitution additive for animal feed and preparation method thereof
CN112617030A (en) * 2020-12-10 2021-04-09 湖北雅琪生物科技有限公司 Compound traditional Chinese medicine extract feed additive and preparation method thereof
CN113951373A (en) * 2021-10-21 2022-01-21 成都师范学院 Preparation method and application of fermentation type compound acidifier
CN114073733A (en) * 2021-09-09 2022-02-22 潘义泰(广州)生物科技有限公司 Nano traditional Chinese medicine for replacing antibiotics

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CN103315155A (en) * 2013-06-21 2013-09-25 华南农业大学 Application of phytol to preparation of pig feed additive and pig feed
CN103392922A (en) * 2013-07-19 2013-11-20 安佑生物科技集团有限公司 Compound feed additive capable of improving health level of animal
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CN104206707A (en) * 2014-07-24 2014-12-17 河南雄峰科技有限公司 Core material of antibiotic-free feed and feed prepared by employing same
CN104222630A (en) * 2014-09-09 2014-12-24 上海邦成生物工程有限公司 Coated acidifying agent for feed and preparation method thereof
CN104397409A (en) * 2014-11-24 2015-03-11 山东龙海生物科技有限公司 Biological antimicrobial-free complete feed for medium and big pigs and preparation method of biological antimicrobial-free complete feed
CN104397351A (en) * 2014-11-24 2015-03-11 山东龙海生物科技有限公司 Biological antimicrobial-free feed additive and preparation method thereof
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CN105104786A (en) * 2015-07-24 2015-12-02 湖南优久农牧有限公司 Pigling Chinese herbal medicine probiotics anti-diarrhoea complete formula feed and preparation method therefor
CN105767532A (en) * 2016-03-22 2016-07-20 江西天佳动物药业有限公司 Compound plant essential oil-type feed additive capable of replacing antibiotics
CN106343155A (en) * 2016-04-14 2017-01-25 安徽华瑞医药技术开发有限公司 Preparation method for functional feed additive
CN105941902A (en) * 2016-04-29 2016-09-21 湖南九鼎科技（集团）有限公司 Antibiotic-free feed for fattening-stage pigs and preparation method thereof
CN105941902B (en) * 2016-04-29 2019-11-05 湖南九鼎科技（集团）有限公司 A kind of nonreactive feed in growing and fattening pigs stage and preparation method thereof
CN106359902A (en) * 2016-09-22 2017-02-01 广西大学 Traditional Chinese medicine feed additive capable of replacing antibiotics
CN106578386A (en) * 2016-12-20 2017-04-26 河南科技学院 Thymol new application
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CN110973357A (en) * 2019-12-31 2020-04-10 宜兴市天石饲料有限公司 Slow-release type compound acidifier and preparation method thereof
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