CN106359849A - Fermented feed containing humic acid chelated trace elements and preparation method thereof - Google Patents
Fermented feed containing humic acid chelated trace elements and preparation method thereof Download PDFInfo
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- CN106359849A CN106359849A CN201610739906.4A CN201610739906A CN106359849A CN 106359849 A CN106359849 A CN 106359849A CN 201610739906 A CN201610739906 A CN 201610739906A CN 106359849 A CN106359849 A CN 106359849A
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- fermented feed
- trace element
- humic acid
- humic
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Links
- 239000011573 trace mineral Substances 0.000 title claims abstract description 71
- 235000013619 trace mineral Nutrition 0.000 title claims abstract description 71
- 239000004021 humic acid Substances 0.000 title claims abstract description 66
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 title claims abstract description 64
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 82
- 230000004151 fermentation Effects 0.000 claims abstract description 82
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 21
- 239000000463 material Substances 0.000 claims abstract description 20
- 241000446281 Eschenbachia blinii Species 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 239000004575 stone Substances 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 238000007792 addition Methods 0.000 claims abstract description 5
- 239000003814 drug Substances 0.000 claims description 37
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 22
- 239000011701 zinc Substances 0.000 claims description 22
- 229910052725 zinc Inorganic materials 0.000 claims description 22
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 21
- 229910052802 copper Inorganic materials 0.000 claims description 21
- 239000010949 copper Substances 0.000 claims description 21
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 16
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 16
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 16
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 15
- 229910052748 manganese Inorganic materials 0.000 claims description 15
- 239000011572 manganese Substances 0.000 claims description 15
- 239000000047 product Substances 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 15
- 241000721156 Chloranthus spicatus Species 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 13
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- 239000012467 final product Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
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- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 239000013522 chelant Substances 0.000 abstract description 19
- 230000009920 chelation Effects 0.000 abstract description 10
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- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 150000001413 amino acids Chemical class 0.000 abstract description 4
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- 239000004455 soybean meal Substances 0.000 abstract 1
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- 238000011160 research Methods 0.000 description 5
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 4
- 235000019750 Crude protein Nutrition 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
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- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 description 3
- 241000721047 Danaus plexippus Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 description 3
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- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
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- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 2
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000066076 Calliurichthys japonicus Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 206010027514 Metrorrhagia Diseases 0.000 description 1
- 208000010359 Newcastle Disease Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010030302 Oliguria Diseases 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 206010053476 Traumatic haemorrhage Diseases 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- 239000012675 alcoholic extract Substances 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
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- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
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- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
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- 208000001780 epistaxis Diseases 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
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- 230000002550 fecal effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
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- 229910052738 indium Inorganic materials 0.000 description 1
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- 206010022437 insomnia Diseases 0.000 description 1
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- 231100000566 intoxication Toxicity 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 150000002611 lead compounds Chemical class 0.000 description 1
- 239000003077 lignite Substances 0.000 description 1
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- 210000004072 lung Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
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- 229910052749 magnesium Inorganic materials 0.000 description 1
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- 230000027939 micturition Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
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- 210000003205 muscle Anatomy 0.000 description 1
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- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 125000004151 quinonyl group Chemical group 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000009781 safety test method Methods 0.000 description 1
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- 230000028327 secretion Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
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- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The present invention discloses a fermented feed containing humic acid chelated trace elements. Bacterium strains are inoculated into a fermentation medium containing the humic acid chelated trace elements and the mixture is fermented to obtain the fermented feed containing the humic acid chelated trace elements. The fermentation medium is composed of fermentation materials and water. The raw materials of the fermentation materials consist of 10-20 parts of soybean meal, 20-45 parts of bran, 5-10 parts of humic acids, 40-50 parts of stone powder, 5-10 parts of chloranthus fortunei, 5-10 parts of chloranthus japonicus, 4-8 parts of apocynum venetum leaves, 4-8 parts of conyza blinii, 4-8 parts of folium microcotis and 0.7-1.4 parts of the humic acid chelated trace elements. The present invention also discloses a preparation method of the fermented feed. The fermented feed is prepared by the chelation of the humic acids and trace elements, helps alleviate the problems of resource wastes and environmental pollutions caused by the additions of the inorganic trace elements, and solves the problems that the amino acid trace element chelate is high in production costs and not conducive to wide uses.
Description
Technical field
The present invention relates to a kind of fermented feed containing humic acid chelating trace element and preparation method thereof, belong to fermented feed
Technical field.
Background technology
Mineral element is that animal sustains life and produces one of requisite nutrient of product, is included according to animal body
The difference of amount, mineral is generally divided into two classes: macroelement refers to account for the inorganic elementss of animal body total amount ten thousand/above,
As calcium, phosphorus, sodium, potassium, magnesium, sulfur etc.；Trace element refer to those in animal body content not as good as body weight ten thousand/ chemistry
Element, such as ferrum, copper, cobalt, manganese, zinc etc..Though trace element content in animal feed is few, they directly or indirectly participate in machine
The nearly all physiology of body and biochemical process, in the digestion, the growth of breeding, immune system and skeleton, muscle and tooth of poultry
All play an important role, closely related with growth of animal and health, therefore Animal nutrition and feedstuff industry are it is widely recognized that moving
In thing daily ration, trace element supplement is necessary.
Trace element makes an addition to, with what chemical state, the emphasis class always studied in recent decades both at home and abroad in feedstuff
One of topic.The initial period of application, trace element is all to be added in feedstuff in the form of inorganic salt, but research shows, inorganic
Salt trace element unstable chemcial property itself, having catalysis and oxidation catalysiss effect, thus destroying vitamin, reducing some medicines
The potency of thing, directly results in quality of the fodder and safety declines；And the biological value of inorganic salt microelement is relatively low, with sulfur
As a example sour copper, ablactational baby pig is only 5%～20% to its absorbance, Adult Pig not higher than 5%～10%, and major part passes through feces
Discharge, cause the wasting of resources, also pollute environment simultaneously.Due to the many disadvantages of inorganic salt microelement, external expert early in
The sixties in 20th century has started the research of organic trace element product, and the such product of studies in China then started from the eighties in 20th century
Phase.
Aminoacid chelating microelement is third generation product, has good absorbing effect, is the 4 of inorganic microelement absorbance
Times, steady chemical structure, the advantages of biological value is high, but aminoacid chelating microelement is expensive, is unfavorable for extensively making
With.
Humic acidss are the macromolecular compounds that a class is extracted from peat, brown coal or weathered coal, according to its dissolubility, color
And molecular size range, humic acid, ulmic acid and fulvic acid can be classified as.The essential element of humic acidss consist of c, h, o, n and
S, relative density is between 1.330-1.448.Humic acidss have many active groups, such as carbonyl, phenolic hydroxyl group, alcoholic extract hydroxyl group, carboxyl, alkene
Alcohol radical, sulfonic group, amido, quinonyl and methoxyl group etc., have faintly acid, hydrophilic, cation exchange, complexing power and higher
Absorbability, can be with k+、na+、ca2+、mg2+And nh4+In conjunction with the corresponding humate of generation.Humic acidss are as fertilizer production profit
With existing history for many years, at home and abroad it has been widely popularized use；But, because humic acidss molecular weight is big, biological active matter
Matter is few, and nutritional labeling is low, and discomfort is fit to do the culture matrix of biological feedstuff, and the therefore research for humic acid fermentation feedstuff is very few.
Content of the invention
For above-mentioned prior art, it is an object of the invention to provide a kind of fermentation containing humic acid chelating trace element is raised
Material, this fermented feed passes through the chelating of humic acidss and trace element, contributes to alleviating the money that the interpolation of inorganic microelement causes
Source wastes and problem of environmental pollution, and solves that Chelates of Amino Acids And Trace Elements producing cost is high, it is widely used to be unfavorable for
Problem.
It is a further object of the present invention to provide this contains the preparation method of the fermented feed of humic acid chelating trace element.
For achieving the above object, the present invention adopts following technical proposals:
It is an object of the present invention to provide a kind of fermented feed containing humic acid chelating trace element.
The fermented feed that the present invention provides, is inoculation bacterium in the fermentation medium containing humic acid chelating trace element
Kind, fermentation, obtain the fermented feed containing humic acid chelating trace element.
In above-mentioned fermented feed, described humic acid chelating trace element is zinc humic acid, copper humate and humic acidss manganese
One or more of.Preferably, described humic acid chelating trace element be by zinc humic acid, copper humate and humic acidss manganese by
Mass ratio (1.4-3.6): (0.086-0.215): (6-10) mixes.
In above-mentioned fermented feed, described fermentation medium is made up of fermentation material and water；The raw material group of described fermentation material
Become:
Bean cake 10-20 part, wheat bran 20-45 part, humic acidss 5-10 part, stone powder 40-50 part, silk fringe chu lan tree 5-10 part, silver
Line grass 5-10 part, Folium Apocyni Veneti 4-8 part, conyza blinii 4-8 part, Folium Microcoris paniculatae 4-8 part, humic acid chelating trace element 0.7-1.4
Part.
In above-mentioned fermented feed, the mass percent that described fermentation material accounts for fermentation medium is 50-60%, and water accounts for fermentation
The mass percent of culture medium is 40-50%.
In above-mentioned fermented feed, described strain is Lactobacillus plantarum.Preferably, described Lactobacillus plantarum is plant breast bar
Bacterium lp (lactobacillus plantarum), described Lactobacillus plantarum lp (lactobacillus plantarum), in
It is preserved in China typical culture collection center within 06 21st, 2010, its deposit number is: cctcc m 2010150；Record
Another patent " one plant of lactic acid bacteria that can be used for biological preservation and its application " (cn 101914475 a) in applicant
In.
It is a further object to provide a kind of prepare the above-mentioned fermented feed containing humic acid chelating trace element
Method.
The method that the present invention provides, comprises the steps:
(1) silk fringe chu lan tree, Herba chloranthi japonici, Folium Apocyni Veneti, conyza blinii and Folium Microcoris paniculatae be dry, pulverize, mixing, in making
Medicated powder；
(2) Chinese medicine powder of step (1) is mixed with bean cake, wheat bran, humic acidss, stone powder and humic acid chelating trace element,
Add water, stir, prepare fermentation medium, and to adjust the water content of fermentation medium be 40-50%, ph is 6.0-
7.0；
(3) in fermentation medium inoculate Lactobacillus plantarum, fermented at 30-42 DEG C, when fermented product ph be down to 4.0 with
When lower, terminate fermentation, obtain final product fermented feed.
In step (2), the preparation method of described humic acid chelating trace element is:
By the inorganic ion of humic acidss and trace element in molar ratio (1-3): (1-2) is added to the water, adjusting ph is
4-6, at 60-80 DEG C, under stirring condition, reacts 1-2h, obtains the trace element chelated liquid of humic acidss；Then by micro for humic acidss unit
Element chelating liquid concentrates, and adds the absolute ethyl alcohol and stirring of 2-3 times of volume uniformly, washing remove the inorganic salt of free trace element from
Son, centrifugation discards supernatant, drying precipitate, obtains final product.
The present invention is to the ratio of inorganic ion addition of humic acidss and trace element during chelating, ph condition and instead
The Parameter Conditions such as temperature are answered to be optimized investigation, result shows the chelating side of the humic acidss using the present invention and trace element
Method, can greatly improve the chelation percent of trace element.
In step (3), the addition of Lactobacillus plantarum is 5 × 105-5×106Cfu/g, adds in every gram of fermentation material
The viable count of Lactobacillus plantarum is 5 × 105-5×106cfu.
The fermented feed containing humic acid chelating trace element of the present invention is that fermented production prepares, and therefore ferments
Process conditions for fermented feed performance impact it is critical that.Specifically:
Fermentation medium is to be used for thalli growth, breeding and synthetic product, contains corruption in the fermentation medium of the present invention
Phytic acid chelated microelement, as one of matrix components, after Lactobacillus plantarum fermentation, on the one hand can promote the growth of thalline,
Improve protein content and nutritive value in tunning；On the other hand, improve the content of fulvo acid in fermented feed,
It is more beneficial for the absorption to trace element for the body.
Also herbal component is contained, this herbal component one side can be plant breast bar in the fermentation medium of the present invention
The growth and breeding of bacterium provides carbon source and nutrient substance；On the other hand, Lactobacillus plantarum can carry out biological to Chinese herbal medicine
Ferment converts, and sweat completes a series of reaction that chemosynthesis are difficult to, and fermentation produces new compound or activity is higher
Lead compound, and multiple secondary metabolites can be produced, the change property of medicine by a larger margin, by the macromolecular complex in Chinese herbal medicine
Matter is converted into the small-molecule substance that can be absorbed rapidly by body intestinal, and both interact, and enhances answering of Chinese herbal medicine
Use effect.
Herbal component in fermentation medium of the present invention contains multi-flavor crude drug, and the compatibility of Chinese medicine is particular about mutual reinforcement between, phase
Make, mutual restraint between two drugs, the mutual-detoxication, the mutual inhibition conversely, because there are different compatibility relationships between different Chinese medicine, therefore using two tastes with
It is necessary to be allowed a choice during upper Chinese medicine, give full play to the compatibility relationship that between medicine, mutual reinforcement between phase makes.In the herbal component of the present invention
By kinds of traditional Chinese medicines is carried out compatibility, between medicine, jointly play the effect of promoting blood circulation to remove blood stasis, heat-clearing and toxic substances removing, enhancing immunity,
There is obvious potentiation, given full play to the compatibility relationship that mutual reinforcement between phase makes.In medicament composing prescription, the pharmacology analysis of each taste medicine are such as
Under:
Silk fringe chu lan tree c.fortune (a.gray) solms: perennial herb, herb is medicinal, energy promoting blood circulation to remove blood stasis, heat clearing away
Removing toxic substances.
Herba chloranthi japonici c.japonicas sieb: perennial herb, herb is medicinal, can qi-regulating, promoting blood circulation, dissipating blood stasis, removing toxic substances.
Folium Apocyni Veneti: sweet, bitter, cold.Return liver warp.Suppressing the hyperactive liver is calmed the nerves, clearing away heat and promoting diuresiss.For dizziness due to hyperactivity of liver-YANG, palpitation and insomnia, edema
Oliguria.
Conyza blinii: bitter, tremble with fear.Return lung, Liver Channel.Removing heat-phlegm, relieving cough and asthma, detoxification and promoting urination, cooling blood for hemostasis.For lung-heat
Cough, abundant expectoration is panted, pharyngalgia, aphtha, jaundice due to damp-heat, and epistaxis is had blood in stool, metrorrhagia, traumatic hemorrhage.
Folium Microcoris paniculatae: micro- acid, cool.Returns spleen, stomach.Relieving dyspepsia, clearing away heat-damp and promoting diuresis.For eating accumulation, cold, fever is damp and hot
Jaundice.
In said herbal medicine component, with silk fringe chu lan tree, Herba chloranthi japonici as monarch drug, Folium Apocyni Veneti, conyza blinii are ministerial drug, respectively
Taste Chinese medicine synergism, plays anti-bacteria and anti-virus, effect of enhancing human body immunity power jointly.
In the herbal component of the present invention, each crude drug is an organic whole, indispensable.Inventor was researching and developing
Find in journey, reduce any one of said herbal medicine component crude drug, or there is the crude drug of the similar property of medicine to this
The raw material components of bright Chinese medicine composition are replaced, then the overall action effect of herbal component significantly reduces；The present invention's
It is further added by other raw material components, the whole structure of fermented feed does not improve significantly, very on the basis of herbal component
Occur to the situation that the whole structure having fermented feed reduces.
In sweat, the ph value of fermentation liquid is the aggregative indicator of microorganism metabolic activity under the conditions of certain environment, is one
The important fermentation parameter of item, it has a great impact to the growth of thalline and the accumulation of product.The present invention is to ph in sweat
Value is optimized investigation, and using the ph value of fermented product as the key index controlling fermentation reaction.Result shows, using this
Bright ph condition carries out fermentation reaction, can significantly improve the effective ingredient in fermented feed.
The synthesis of the growth of microorganism and product is all to carry out under the catalysis of various enzymes, and temperature is to ensure that enzymatic activity
Essential condition, therefore fermentation system must assure that stable and suitable temperature environment.The present invention has carried out excellent to the temperature of fermentation
Change investigate, result shows, using the temperature conditionss of the present invention, on the one hand can ensure that the activity of enzyme, be unlikely to because temperature too high
Lead to the inactivation of enzyme；On the other hand, the growth of thalline and the synthesis of product can farthest be improved.
Beneficial effects of the present invention:
The present invention utilizes probiotics fermention technology, adds humic acid chelating trace element in the fermentation medium, can promote
Enter the growth of thalline, improve protein content and the nutritive value of tunning.Contain humic acid chelating using prepared by the present invention
The fermented feed feeding dorking of trace element, can significantly reduce the feedstuff-meat ratio of feedstuff, reduce aquaculture cost；Can also improve
Index and spleen index and bursal index, enhance the immunity of body.
Specific embodiment
The present invention is further illustrated in conjunction with the embodiments it should explanation, and following embodiments are merely to explain
The present invention, is not defined to its content.
Embodiment 1: the chelating technical study of trace element and humic acidss
1. the chelating of humic acidss and zinc
Specific process for sequestration is as follows:
By humic acidss (fulvic acid, commercially available prod) and zn2+According to mol ratio 1:1 (1kg humic acidss: 50g znso4·
7h2O) ratio adds in 50l water, and adjusting ph is 5, under 80 DEG C, stirring condition, reacts 1h, obtains zinc humic acid chelating liquid.
Then zinc humic acid chelating liquid is concentrated under the conditions of 60 DEG C, take out when chelating liquid and be sticky and stiff, add and (divide many
Secondary) absolute ethyl alcohol and stirring of 100l mixes, and removes free state zinc for washing, centrifugation discards supernatant, and precipitate is complete in baking oven
Full drying, obtains zinc humic acid chelate.
Using the chelation percent of atomic absorption spectroscopy determination chelate, method particularly includes:
Atomic absorption spectrophotometry: weigh 0.5g and dry chelate in Muffle furnace ashing 5h, be settled to 50% hydrochloric acid
50 milliliters, the total zinc amount in detection solution；0.5g is separately taken to dry chelate with, after 50 milliliters of absolute ethanol washings, being centrifuged, precipitation
Thing Muffle furnace is ashed 5h, is settled to 50 milliliters with 50% hydrochloric acid, the pure chelated microelements in detection chelate.After testing,
The chelation percent of zinc humic acid chelate is 93.79%, and in chelate, the content of zinc is 9.72mg/g, and the response rate is 63.29%.
2. the chelating of humic acidss and manganese
Specific process for sequestration is as follows:
By humic acidss and mn2+It is 3:1 (3kg humic acidss: 30g mnso according to mol ratio4·h2O) ratio adds 50l water
In, adjusting ph is 5, under 80 DEG C, stirring condition, reacts 1h, obtains humic acidss manganese chelating liquid.Then liquid will be chelated at 60 DEG C
Concentrate, take out when chelating liquid and be sticky and stiff, add the absolute ethyl alcohol and stirring of (several times) 100l to mix, for washing
Remove free state manganese, centrifugation discards supernatant, precipitate dried completely in baking oven, obtains humic acid manganic chelates, using above-mentioned
The chelation percent of atomic absorption spectroscopy determination chelate.After testing, the chelation percent of humic acidss manganic chelates is 92.79%,
In chelate, manganese content is 2.44mg/g, and the response rate is 53.38%.
3. the chelating of humic acidss and copper
Specific process for sequestration is as follows:
By humic acidss sample and cu2+It is 1:2 (1kg humic acidss: 100g cuso according to mol ratio4·5h2O) ratio adds
Enter in 50l water, adjusting ph is 5, at 70 DEG C, under stirring condition, react 1h, obtain copper humate chelating liquid.Then liquid will be chelated
Concentrate at 60 DEG C, take out when chelating liquid and be sticky and stiff, add (several times) 100l absolute ethyl alcohol and stirring to mix, use
Remove free state copper to wash, centrifugation discards supernatant, precipitate dried completely in baking oven, obtains copper humate chelate, adopt
Chelation percent with above-mentioned atomic absorption spectroscopy determination chelate.After testing, the chelation percent of copper humate chelate is
96.29%, the copper content of chelate products is 12mg/g, and the response rate is 69.66%.
Comparative example 1: the chelating of trace element and humic acidss
1. the chelating of humic acidss and zinc
By humic acidss (fulvic acid, commercially available prod) and zn2+The mol ratio adding is adjusted to 1:2, and ph value is adjusted to 3, at 50 DEG C
Under the conditions of be stirred, remaining operation, with the chelating of zinc " humic acidss with " in embodiment 1, prepares zinc humic acid chelate,
After testing, the chelation percent of chelate is 81.32%.
2. the chelating of humic acidss and manganese
By humic acidss and mn2+The mol ratio adding is adjusted to 1:1, and ph value is adjusted to 6, is stirred under the conditions of 90 DEG C, its
Remaining operation, with " chelating of humic acidss and manganese " in embodiment 1, prepares humic acidss manganic chelates, after testing, the chela of chelate
Conjunction rate is 78.69%.
3. the chelating of humic acidss and copper
By humic acidss and cu2+The mol ratio adding is adjusted to 1:3, and ph value is adjusted to 6, is stirred under the conditions of 85 DEG C, its
Remaining operation, with " chelating of humic acidss and copper " in embodiment 1, prepares copper humate chelate, after testing, the chela of chelate
Conjunction rate is 84.16%.
By embodiment 1 and comparative example 1 as can be seen that adopting the humic acidss of the present invention and trace element chelated technique, permissible
Significantly improve chelation percent.
Embodiment 2: the preparation of the fermented feed containing humic acid chelating trace element
1. the composition of fermentation medium:
55% fermentation material, 45% water；It is mass percent.
The consisting of of fermentation material:
15 parts of bean cake, 35 parts of wheat bran, 8 parts of humic acidss, 8 parts of part, thread fringe chu lan tree of stone powder 45,6 parts of Herba chloranthi japonici, Folium Apocyni Veneti
6 parts, 5 parts of conyza blinii, 5 parts of Folium Microcoris paniculatae, 1 part of humic acid chelating trace element.
Wherein, humic acid chelating trace element is by zinc humic acid, copper humate and humic acidss manganese 2:0.1:8 in mass ratio
Mix.
Above-mentioned zinc humic acid, copper humate and humic acidss manganese are to prepare according to the process for sequestration of embodiment 1.
2. preparation method is as follows:
(1) silk fringe chu lan tree, Herba chloranthi japonici, Folium Apocyni Veneti, conyza blinii and Folium Microcoris paniculatae be dry, pulverize, mixing, in making
Medicated powder；
(2) Chinese medicine powder of step (1) is mixed with bean cake, wheat bran, humic acidss, stone powder and humic acid chelating trace element,
Add water, stir, prepare fermentation medium, and adjust the water content of fermentation medium be 45%, ph be 6.5；
(3) add Lactobacillus plantarum lp (deposit number is: cctcc m 2010150) mycopowder in fermentation medium, make
Its viable count in fermentation material is 1 × 106Cfu/g, is fermented at 37 DEG C, when fermented product ph is down to below 4.0, terminates
Fermentation, obtains final product fermented feed.
Embodiment 3: the preparation of the fermented feed containing humic acid chelating trace element
1. the composition of fermentation medium:
60% fermentation material, 40% water；It is mass percent.
The consisting of of fermentation material:
10 parts of bean cake, 45 parts of wheat bran, 5 parts of humic acidss, 5 parts of part, thread fringe chu lan tree of stone powder 50,10 parts of Herba chloranthi japonici, Herba Apocyni veneti
4 parts of leaf, 8 parts of conyza blinii, 4 parts of Folium Microcoris paniculatae, 0.7 part of humic acid chelating trace element.
Wherein, described humic acid chelating trace element is by zinc humic acid, copper humate and humic acidss manganese in mass ratio
3.6:0.215:10 mixing.
Above-mentioned zinc humic acid, copper humate and humic acidss manganese are to prepare according to the process for sequestration of embodiment 1.
2. preparation method is as follows:
(1) silk fringe chu lan tree, Herba chloranthi japonici, Folium Apocyni Veneti, conyza blinii and Folium Microcoris paniculatae be dry, pulverize, mixing, in making
Medicated powder；
(2) Chinese medicine powder of step (1) is mixed with bean cake, wheat bran, humic acidss, stone powder and humic acid chelating trace element,
Add water, stir, prepare fermentation medium, and adjust the water content of fermentation medium be 40%, ph be 6.0；
(3) add Lactobacillus plantarum lp (deposit number is: cctcc m 2010150) mycopowder in fermentation medium, make
Its viable count in fermentation material is 5 × 105Cfu/g, is fermented at 30 DEG C, when fermented product ph is down to below 4.0, terminates
Fermentation, obtains final product fermented feed.
Embodiment 4: the preparation of the fermented feed containing humic acid chelating trace element
1. the composition of fermentation medium:
50% fermentation material, 50% water；It is mass percent.
The consisting of of fermentation material:
20 parts of bean cake, 20 parts of wheat bran, 10 parts of humic acidss, 10 parts of part, thread fringe chu lan tree of stone powder 40,5 parts of Herba chloranthi japonici, Herba Apocyni veneti
8 parts of leaf, 4 parts of conyza blinii, 8 parts of Folium Microcoris paniculatae, 1.4 parts of humic acid chelating trace element.
Wherein, described humic acid chelating trace element is by zinc humic acid, copper humate and humic acidss manganese in mass ratio
1.4:0.086:6 mixing.
Above-mentioned zinc humic acid, copper humate and humic acidss manganese are to prepare according to the process for sequestration of embodiment 1.
2. preparation method is as follows:
(1) silk fringe chu lan tree, Herba chloranthi japonici, Folium Apocyni Veneti, conyza blinii and Folium Microcoris paniculatae be dry, pulverize, mixing, in making
Medicated powder；
(2) Chinese medicine powder of step (1) is mixed with bean cake, wheat bran, humic acidss, stone powder and humic acid chelating trace element,
Add water, stir, prepare fermentation medium, and adjust the water content of fermentation medium be 50%, ph be 7.0；
(3) add Lactobacillus plantarum lp (deposit number is: cctcc m 2010150) mycopowder in fermentation medium, make
Its viable count in fermentation material is 5 × 106Cfu/g, is fermented at 42 DEG C, when fermented product ph is down to below 4.0, terminates
Fermentation, obtains final product fermented feed.
Embodiment 5: the nutritional labeling detection of fermented feed:
1. detection method:
Crude protein content measures: using crude protein determining method in gb/t 6432-1994 feedstuff.
Crude fiber content measures: using crude fibre assay method in gb/t 6434-1994 feedstuff.
Viable count measures: adopts the method for plate culture count.
2. testing result:
In fermentation medium before fermentation and after fermentation, the testing result of nutritional labeling is as shown in table 1:
Crude protein, crude fibre, viable count changes of contents before and after table 1 fermentation
| Crude protein content (%) | Crude fiber content (%) | Viable count cfu/g | |
| Before fermentation | 13.6 | 14.3 | 1×106 |
| After fermentation | 28.4 | 8.2 | 3.2×108 |
Embodiment 6: safety testing:
1. acute toxicity test:
With about 20 grams of body weight, male and female half and half kunming mice 40 only as experimental animal, if three treatment groups and a blank
Matched group.Every group 10, male and female half and half.Blank control group feeds basal diet, and treatment group adds the present invention in basal diet
The fermented feed containing humic acid chelating trace element of embodiment 2 preparation, fermented feed the adding in daily ration of three treatment groups
Dosage is respectively 4.0g/kg, 8.0g/kg, 12.0g/kg.Successive administration 7 days, the daily spirit observing mice, appetite, drinking-water and
Intoxication conditions.
Observe situations such as discovery in 7 days, the spirit of the mice of three treatment groups, appetite, drinking-water equal compared with blank control group
Without exception, the situation of mice poisoning does not all occur.Acute toxicity test shows, the fermented feed Clinical practice safety of the present invention.
2. long term toxicity test:
With about 20 grams of body weight, male and female half and half kunming mice 32 only as experimental animal, if three treatment groups and a blank
Matched group.Every group 8, male and female half and half.Blank control group feeds basal diet, and it is real that treatment group adds the present invention in basal diet
Apply the fermented feed containing humic acid chelating trace element of example 2 preparation, the interpolation in daily ration of the fermented feed of three treatment groups
Amount is respectively 1.0g/kg, 2.0g/kg, 3.0g/kg.After successive administration 2 months, each group is stayed 1/4 mice to do convalescent period respectively and is seen
Examine outer, remaining is all put to death, measure indices.Observe and mensure project include as appearance sign and behavioral activity, secretions,
Fecal character；The conventional index of blood and biochemical indicator；The content such as system postmortem and organ coefficient and histological examination.
Result shows: after the fermented feed of feeding Different adding amount continuous to mice, the food-intake to mice and body weight increase
Length all no affects.Toxic action is not all found to hepatic and renal function；The fermented feed safety non-toxic of the present invention is described.
Embodiment 7: effect test: the application in broiler production
1. EXPERIMENTAL DESIGN: after 1 age in days white meat-type chickens chow diet is brooded 7 days, selection body weight is uniform, the basic phase of health status
Near 300 are randomly divided into 3 groups, every group 5 repetitions, and each repeats 20.Specifically it is grouped as follows: organize as matched group, feeding base
Plinth daily ration；Group, adds the fermentation containing humic acid chelating trace element of 2 ‰ embodiment of the present invention 2 preparation in basal diet
Feedstuff；Group, in basal diet add 2 ‰ the fermented feed without humic acid chelating trace element (this fermented feed
Preparation method is identical with the preparation method of the embodiment of the present invention 2, simply chelates micro in the fermentation medium without humic acid
Element).
2. feeding and management:
Each repeating groups test chicken is only with sub-cage rearing, free choice feeding and drinking-water.Before test, hen house, chicken coop are carried out thoroughly
Clean, fumigation.Inoculate newcastle disease vaccine, 28 age in days inoculation bursa of fabricius vaccine in the 7th and the 21st age in days.Formal from the 7th day
Start to test, feed corresponding diet.Temperature control at 22-29 DEG C, relative humidity 55%-60%.Feeding period is 35 days.
3. Testing index:
Growth indexes 21 age in days and 42 ages in days weigh chicken weight and material weight respectively, calculate feedstuff-meat ratio and average daily gain.
Immune indexes 21 age in days and 42 ages in days weigh live chickens body weight respectively, and chicken are massacred and dissects, and weigh spleen
Dirty heavy and fabricius bursa weight, calculates index and spleen index and bursal index.
4. result of the test:
The testing result of growth indexes is as shown in table 2:
Table 2 feedstuff-meat ratio and average daily gain
Represent that difference is not notable (p > 0.05) with column data shoulder mark no letter or same letter, different lowercase letters are poor
Different notable (p < 0.05).
Following table is same.
As can be seen from Table 2,7-21 age in days and 21-42 age in days feedstuff-meat ratio are all minimum to organize, lower than matched group respectively
4.41% (p ＞ 0.05) and 10.91% (p < 0.05)；Ratio organizes low by 3.70% (p ＞ 0.05) and 6.22% (p < 0.05) respectively.
7-21 age in days and 21-42 age in days average daily gain are all to organize highest, higher by 4.28% than matched group respectively (p ＞
0.05) and 8.19% (p < 0.05)；Ratio organizes high by 3.32% (p ＞ 0.05) and 6.47% (p < 0.05) respectively.
The testing result of immune indexes is as shown in table 3:
Table 3 index and spleen index and bursal index
From table 3 it is observed that during 21 age in days, organizing index and spleen index highest, higher by 9.26% than matched group (p ＞ 0.05)；
Group bursal index highest, higher by 4.95% than matched group (p ＞ 0.05).During 42 age in days, group index and spleen index is significantly higher than it
He is two groups, higher by 14.10% than matched group (p < 0.05)；Group bursal index is significantly higher than other two groups, higher than matched group
12.26% (p < 0.05).
In process of the test, all untoward reaction during feeding in the test chicken of group, and containing of the present invention is described
The fermented feed of humic acid chelating trace element is safe and free of toxic and side effects.
Comparative example 2:
The present inventor in R＆D process, the herbal component in fermentation medium has been carried out compatibility relationship investigation and
The research of drug effect, even if it was found that the property of medicine between a few taste crude drug is similar, but the crude drug compatibility different from other makes
With rear, its effect significant difference.For example: the silk fringe chu lan tree in the herbal component of embodiment 2 fermentation medium is replaced
It is changed to Radix Isatidis；Herba chloranthi japonici is replaced with Herba Sarcandrae；Folium Apocyni Veneti is replaced with Caulis Perillae, prepares as described in Example 2
Fermented feed；
Comparative example 3:
In Chinese medicine composition, the dose of each crude drug is the mark efficacy of a drug, even if the medicine composition of prescription is identical, if but medicine
The consumption of thing is different, its efficacy of a drug then have size point, compatibility relationship then has the change of monarch, thus its work(cure mainly then each
Different.The present inventor is carried out to the content of each raw material in the herbal component in fermentation medium in R＆D process
Investigate the research with drug effect, it was found that different proportioning raw materials relations, the especially difference of monarch drug and deal shared by ministerial drug,
Its effect significant difference.For example: by the consumption adjustment of silk fringe chu lan tree in the herbal component of embodiment 2 fermentation medium
For 3 parts, the consumption of Herba chloranthi japonici is adjusted to 15 parts, and the consumption of Folium Apocyni Veneti is adjusted to 15 parts, and the consumption of conyza blinii is adjusted to 15
Part, the consumption of other raw materials is with embodiment 2, and prepares fermented feed as described in Example 2.
Fermented feed prepared by comparative example 2 and comparative example 3 investigates it in broiler production according to the method for " effect test "
In application effect, result shows, the feedstuff-meat ratio of the chicken 21-42 age in days of fermented feed of the feeding embodiment of the present invention 2 preparation
Compared with comparative example 2, reduce 8.4%；Compared with comparative example 3, reduce 6.2%.
The index and spleen index of chicken 42 age in days of fermented feed of the feeding embodiment of the present invention 2 preparation, compared with comparative example 2, carries
High by 11.8%；Compared with comparative example 3, improve 9.7%.
Feeding the embodiment of the present invention 2 preparation chicken 42 age in days of fermented feed bursal index compared with comparative example 2,
Improve 10.4%；Compared with comparative example 3, improve 8.9%.
Illustrate to have between each crude drug collaborative facilitation in the herbal component in fermentation medium of the present invention, not
It is simple replacement or the combination of the raw material with similar quality.The adjustment that the consumption of each raw material is also not conventional can obtain
, in the herbal component in the fermentation medium of the present invention, the consumption proportion of each raw material is according to its property of raw material, Jun Chenzuo
The various Consideration such as compatibility is made to determine, the whole structure for fermented feed plays very important effect.
Claims (9)
1. a kind of fermented feed containing humic acid chelating trace element is it is characterised in that be containing the micro unit of humic acid chelating
Inoculate strain, fermentation in the fermentation medium of element, obtain the fermented feed containing humic acid chelating trace element.
2. fermented feed as claimed in claim 1 it is characterised in that described humic acid chelating trace element be zinc humic acid,
One or more of copper humate and humic acidss manganese.
3. fermented feed as claimed in claim 2 is it is characterised in that described humic acid chelating trace element is by humic acidss
Zinc, copper humate and humic acidss manganese (1.4-3.6) in mass ratio: (0.086-0.215): (6-10) mixes.
4. the fermented feed as described in any one of claims 1 to 3 is it is characterised in that described fermentation medium is by fermentation material
With water composition；The raw material of described fermentation material consists of:
Bean cake 10-20 part, wheat bran 20-45 part, humic acidss 5-10 part, stone powder 40-50 part, silk fringe chu lan tree 5-10 part, Herba chloranthi japonici
5-10 part, Folium Apocyni Veneti 4-8 part, conyza blinii 4-8 part, Folium Microcoris paniculatae 4-8 part, humic acid chelating trace element 0.7-1.4 part.
5. fermented feed as claimed in claim 4 is it is characterised in that described fermentation material accounts for the mass percent of fermentation medium
For 50-60%, the mass percent that water accounts for fermentation medium is 40-50%.
6. fermented feed as claimed in claim 1 is it is characterised in that described strain is Lactobacillus plantarum；It is preferably plant breast
Bacillus lp, deposit number is: cctcc m 2010150.
7. the preparation method of the fermented feed described in any one of claim 1-6 is it is characterised in that step is as follows:
(1) silk fringe chu lan tree, Herba chloranthi japonici, Folium Apocyni Veneti, conyza blinii and Folium Microcoris paniculatae be dry, pulverize, mixing, make Chinese medicine
Powder；
(2) Chinese medicine powder of step (1) is mixed with bean cake, wheat bran, humic acidss, stone powder and humic acid chelating trace element, add
Water, stirs, and prepares fermentation medium, and to adjust the water content of fermentation medium be 40-50%, and ph is 6.0-7.0；
(3) inoculate Lactobacillus plantarum in fermentation medium, fermented at 30-42 DEG C, when fermented product ph is down to less than 4.0
When, terminate fermentation, obtain final product fermented feed.
8. preparation method as claimed in claim 7 is it is characterised in that in step (2), described humic acid chelating trace element
Preparation method is:
By the inorganic ion of humic acidss and trace element in molar ratio (1-3): (1-2) is added to the water, regulation ph is 4-6,
At 60-80 DEG C, under stirring condition, react 1-2h, obtain the trace element chelated liquid of humic acidss；Then by humic acidss trace element chela
Close liquid to concentrate, add the absolute ethyl alcohol and stirring of 2-3 times of volume uniformly, washing removes the inorganic ion of free trace element, from
The heart discards supernatant, drying precipitate, obtains final product.
9. preparation method as claimed in claim 7 is it is characterised in that in step (3), and the addition of Lactobacillus plantarum is 5 ×
105-5×106cfu/g.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108651758A (en) * | 2018-07-25 | 2018-10-16 | 石阡县毅峰农牧开发有限公司 | One kind chicken feed containing phytase and preparation method thereof |
| CN112515044A (en) * | 2020-12-01 | 2021-03-19 | 北京挑战农业科技有限公司 | Preparation method and application of fermentation premix capable of enhancing vitamin stability |
| CN113180151A (en) * | 2021-05-25 | 2021-07-30 | 北京博思汇科生物技术有限公司 | Method for producing organic trace zinc humate as feed additive |
| CN113439802A (en) * | 2021-05-25 | 2021-09-28 | 北京博思汇科生物技术有限公司 | Method for producing feed additive organic trace humic acid copper |
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| CN1135846A (en) * | 1995-05-11 | 1996-11-20 | 湖南化工研究院 | Preparation and application of pre-mixture for An, Mn, Cu and Fe sulfate humic acid |
| CN104335910A (en) * | 2014-10-10 | 2015-02-11 | 安徽万利生态园林景观有限公司 | Novel edible pig house pad materials and preparation method of pig house pad bed |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1135846A (en) * | 1995-05-11 | 1996-11-20 | 湖南化工研究院 | Preparation and application of pre-mixture for An, Mn, Cu and Fe sulfate humic acid |
| CN104335910A (en) * | 2014-10-10 | 2015-02-11 | 安徽万利生态园林景观有限公司 | Novel edible pig house pad materials and preparation method of pig house pad bed |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108651758A (en) * | 2018-07-25 | 2018-10-16 | 石阡县毅峰农牧开发有限公司 | One kind chicken feed containing phytase and preparation method thereof |
| CN112515044A (en) * | 2020-12-01 | 2021-03-19 | 北京挑战农业科技有限公司 | Preparation method and application of fermentation premix capable of enhancing vitamin stability |
| CN113180151A (en) * | 2021-05-25 | 2021-07-30 | 北京博思汇科生物技术有限公司 | Method for producing organic trace zinc humate as feed additive |
| CN113439802A (en) * | 2021-05-25 | 2021-09-28 | 北京博思汇科生物技术有限公司 | Method for producing feed additive organic trace humic acid copper |
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