CN106819515A - A kind of weanling pig composite probiotics feed additive and application - Google Patents
A kind of weanling pig composite probiotics feed additive and application Download PDFInfo
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- CN106819515A CN106819515A CN201710139855.6A CN201710139855A CN106819515A CN 106819515 A CN106819515 A CN 106819515A CN 201710139855 A CN201710139855 A CN 201710139855A CN 106819515 A CN106819515 A CN 106819515A
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- cfu
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- lactobacillus acidophilus
- bacillus subtilis
- weanling pig
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- 239000006041 probiotic Substances 0.000 title claims abstract description 21
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 21
- 239000003674 animal food additive Substances 0.000 title claims abstract description 15
- 239000002131 composite material Substances 0.000 title claims abstract description 15
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 41
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 41
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 41
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 34
- 239000000654 additive Substances 0.000 claims abstract description 32
- 230000000996 additive effect Effects 0.000 claims abstract description 32
- 235000005911 diet Nutrition 0.000 claims abstract description 27
- 230000037213 diet Effects 0.000 claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 17
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- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 16
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- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 15
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- 241000894006 Bacteria Species 0.000 claims abstract description 13
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- 230000000903 blocking effect Effects 0.000 claims description 12
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- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 6
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 6
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- 150000001615 biotins Chemical class 0.000 description 6
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- 229910052742 iron Inorganic materials 0.000 description 6
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 6
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- 229910052725 zinc Inorganic materials 0.000 description 6
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- 238000007792 addition Methods 0.000 description 5
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- 206010059866 Drug resistance Diseases 0.000 description 3
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- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
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- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 206010008631 Cholera Diseases 0.000 description 1
- 241000710777 Classical swine fever virus Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 108060003951 Immunoglobulin Proteins 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
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- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
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- 239000001530 fumaric acid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
Abstract
The present invention discloses a kind of weanling pig composite probiotics feed additive and application.Including corn 58 65, dregs of beans 16 24, whey powder 25, fish meal 48, wheat bran 15, glucose 13, acidulant 0.1 0.5, calcium monohydrogen phosphate 0.5 1.5, stone flour 0.5 1.2, salt 0.1 0.6, L FE-5s 0.1 0.8, DL methionine 0.1 0.7, threonine 0.1 0.7, piglet premix 14, lactobacillus acidophilus 0.001 0.01, bacillus subtilis 0.001 0.01, viable bacteria content is 1 × 10 in two kinds of freeze-dried powders9CFU/g（Concentration is 1 × 10 in diet4 CFU/g~1×105CFU/g）.Result shows：The lactobacillus acidophilus and bacillus subtilis compound probiotic additive that proper proportion is added in Weaned Growing Pig Diets have the effect for significantly improving Growth Performance of Weaning Piglets and immunity.
Description
Technical field
The invention belongs to farm animals nutrition technique research field, it is related to one kind with lactobacillus acidophilus and bacillus subtilis
Bacterium is the composite probiotics feed formula of additive, is in particular a kind of raising Growth Performance of Weaning Piglets and immunity etc.
The application of aspect.
Background technology
Antibioticses growth promoter is the biosynthesis by the structural material peptide glycan of interference cell wall so as to suppress
The breeding of bacterium.At present, under the bacterial drug resistance, medicament residue, the animal immunizing power that are caused due to abuse of antibiotics in aquaculture
The problems such as drop, has had a strong impact on the Safety of Livestock Products ' Quality of China, also brings extreme loss to economic growth.In recent years, protect
Barrier piglet is healthy, improve focus and difficult point that the aspects such as its immunity are increasingly becoming pig nutrition and feed research field, and feed adds
Plus the research of agent also towards noresidue, have no drug resistance and the direction of human health and environment non-hazardous developed.Feed adds
Plus agent lactobacillus acidophilus and bacillus subtilis are because its distinctive multi-functional, prebiotic effect and to be not likely to produce drug resistance etc. excellent
Point is widely used in animal productiong.
Lactobacillus acidophilus belongs to Strains of lactobacillus, is primarily present in small intestine, release lactic acid, acetic acid and some are right
The antibiotic that harmful bacteria works, by competing nutriment, suppresses enteron aisle undesirable microorganism propagation；With being carried for animal body
For nutriment and various enzymes, animal digestion is promoted to absorb, the function such as enhancing immunity of organisms.Bacillus subtilis has to be adjusted
Section intestinal microecology balance, promotion are digested and assimilated, improve the functions such as efficiency of feed utilization, enhancing animal immunizing power.Lactobacillus acidophilus
With bacillus subtilis as external source probio have it is pollution-free, without hazard residue, be adapted to enteron aisle existence etc. characteristic, and the two
With similar biological characteristics, thus animal husbandry is gradually applied to more and more widely as one of Substitutes For Antibiotic is supported
Grow in producing.
Consult data in recent years and combine actual conditions, show：Weanling pig nutrition is not only the focus of pig industry,
It is difficult point, the novelty in piglet growth performance and this field of immunity awaits improving.By lactobacillus acidophilus and withered grass bud
Digesting and assimilating and strengthen the functions such as immunoglobulin expression for the nutriment of spore bacillus regulation body, breaks we have studied a kind of
Milk piglet composite probiotics feed additive, piglet growth performance and immunity are improved with this, improve support to a certain extent
Grow the economic benefit of industry.
The content of the invention
It is an object of the invention to disclose a kind of weanling pig composite probiotics feed additive and application.
Another object of the present invention is that to disclose prepared diet thin in Growth Performance of Weaning Piglets, blood T lymphs
Application effect in terms of born of the same parents' subgroup and serum antibody against swine fever virus.
To achieve the above object, the invention discloses following technology contents
A kind of weanling pig composite probiotics feed additive, it is characterised in that using lactobacillus acidophilus and withered in feedstuff
Careless bacillus feed additive, carries out reasonable preparation daily ration, is made up of the raw material of following portions by weight：
Corn 58-65 dregs of beans 16-24 whey powders 2-5
Fish meal 4-8 wheat bran 1-5 glucose 1-3
Acidulant 0.1-0.5 calcium monohydrogen phosphate 0.5-1.5 stone flours 0.5-1.2
Salt 0.1-0.6 L FE-5 0.1-0.8 DL- METHIONINEs 0.1-0.7
Threonine 0.1-0.7 piglet premixes 1-4
Lactobacillus acidophilus additive 0.001-0.01（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g, in diet concentration be 1 ×
104~1×105CFU/g）
Bacillus subtilis bacteria additive 0.001-0.01（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g, concentration is 1 in diet
×104~1×105CFU/g）.
Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper, 410
Mg manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100 mg
VE, 30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
Acidulant of the present invention refers to Anhui Hua Yang agriculture and animal husbandries Science and Technology Ltd. product, and main component is lactic acid, just
Phosphoric acid, citric acid, fumaric acid, formic acid.
The preparation method of Weanling pig compound feed of the present invention：Corn, two kinds of raw materials of dregs of beans are crushed, other are former
Material need not be crushed, and be weighed respectively according to part by weight, be mixed in multifunctional mini-mixer.
The preferred formula of the present invention one（Parts by weight）：
The whey powder 4.5 of 62.698 dregs of beans of corn 18
The glucose 3 of 4 wheat bran of fish meal 4
The stone flour 0.65 of 0.4 calcium monohydrogen phosphate of acidulant 1.1
The DL- METHIONINE 0.1 of 0.3 L lysine HCL of salt 0.15
The piglet premix 1 of threonine 0.1
Lactobacillus acidophilus additive 0.001（Lactobacillus acidophilus concentration is 1 × 10 in diet4CFU/g）
Bacillus subtilis bacteria additive 0.001（Bacillus subtilis bacteria concentration is 1 × 10 in diet4CFU/g）；
Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper, 410 mg
Manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100 mg VE,
30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
Presently preferred formula（Parts by weight）：
The whey powder 4.5 of 62.23 dregs of beans of corn 17
The glucose 3.5 of 4 wheat bran of fish meal 5
The stone flour 0.65 of 0.4 calcium monohydrogen phosphate of acidulant 1.1
The DL- METHIONINE 0.1 of 0.3 L lysine HCL of salt 0.1
The piglet premix 1 of threonine 0.1
Lactobacillus acidophilus additive 0.01（Lactobacillus acidophilus concentration is 1 × 10 in diet5CFU/g）
Bacillus subtilis bacteria additive 0.01（Bacillus subtilis bacteria concentration is 1 × 10 in diet5CFU/g）；
Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper, 410 mg
Manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100 mg VE,
30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
Grown as raising weanling pig the present invention further discloses weanling pig composite probiotics feed additive
Application in performance and immunity.Test index：The average daily gain of weanling pig（ADG）, average daily gain（ADFI）With
Feed-weight ratio（F/G）, t lymphocyte subset group CD4 in whole blood+、CD8+Ratio and CD4+/CD8+Ratio, serum in hog cholera
Poison（CSFV）Antibody blocking rate, so as to illustrate the influence to pigling immunity.
Weanling pig composite probiotics feed additive result of the test of the invention shows：
（1）Compared with control group, a certain amount of lactobacillus acidophilus and bacillus subtilis are added in 6 groups of daily rations of experiment, significantly（P
＜ 0.05）ADG, ADFI of piglet are improve, significantly（P ＜ 0.05）F/G is reduced, shows that the feed formula can promote wean young
The absorption of pig nutriment, effectively improves the growth performance of weanling pig；
（2）Compared with control group, lactobacillus acidophilus and bacillus subtilis are added in 6 groups of daily rations of experiment, in significantly improving whole blood
CD4+/CD8+Ratio, extremely significantly（P ＜ 0.01）CSFV antibody blocking rates in serum are improved, shows that this feed addictive can have
Effect improves weanling pig health status, improves weanling pig immunity.
In order to illustrate further the positive effect of the present invention, there is provided following experiment：
1. experimental animal and packet
Designed using single factor experiment, choose health " Du × long × big " three way cross wean of the kg of body weight 7.47 ± 0.62
Piglet 168, clinical health, development are normal, are randomly divided into 7 treatment, and each treatment is divided into 4 repetitions, and each repeats 6
Pig.7 treatment groups feed respectively：
1）Control group：Basic drawing（Without bacterium）；
2）Test 2 groups：The lactobacillus acidophilus additive of Basic drawing+0.001（Concentration is 1 × 104CFU/g）；
3）Test 3 groups：The bacillus subtilis bacteria additive of Basic drawing+0.001（Concentration is 1 × 104CFU/g）；
4）Test 4 groups：The lactobacillus acidophilus additive of Basic drawing+0.0005（Concentration is 0.5 × 104CFU/g）+
0.0005 bacillus subtilis bacteria additive（Concentration is 0.5 × 104CFU/g）；
5）Test 5 groups：The lactobacillus acidophilus additive of Basic drawing+0.001（Concentration is 1 × 104CFU/g）+ withered grass gemma
Bacillus additive 0.001（Concentration is 1 × 104CFU/g）；
6）Test 6 groups：The lactobacillus acidophilus additive of Basic drawing+0.01（Concentration is 1 × 105CFU/g）+ 0.01 withered grass
Bacillus additive（Concentration is 1 × 105CFU/g）；
7）Test 7 groups：The lactobacillus acidophilus additive of Basic drawing+0.02（Concentration is 2 × 105CFU/g）+ 0.02 withered grass
Bacillus additive（Concentration is 2 × 105CFU/g）.Poisoning manifestations and death condition are observed in experimental period at any time.
Lactobacillus acidophilus and bacillus subtilis are commercially available prod.
2. Screening Experiment of Diet and trophic level
According to NRC（1998）Prepare pig basal diet formula and trophic level is as shown in table 1；
The Basic drawing of table 1 is constituted and trophic level（Feeding basis）
Contain in every kilogram of premixes of a：14,600 mg iron, 630 mg copper, 410 mg manganese, 10,400 mg zinc, 15,000 μ g
Iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3,1,100 mg VE, 30 mg VK3,700 mg VB2,
200 mg VB6,1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
B is according to NRC（1998）Feed ingredient meter is calculated.
3. testing regulations
Experiment is carried out on a large scale commercial product pig farm, 14 days experimental periods.Feeding, management, immune carried out by pig farm established procedure.Raise
After feeding 2 weeks, 3 weanling pig blood samplings are randomly selected from each repeating groups of the optimal treatment group of growth performance, carry out blood
Index（CD4+、CD8+、CD4+/CD8+With Blocking rate）Analysis.The healthy shape of piglet is observed in whole process of the test
Condition, record is dead, wash in a pan piglet and diarrhoea, medicining condition.
4. testing index
Physiochemical indice is determined：After off-test, piglet adopts two pipe blood by vena cava anterior, and after blood sampling, a pipe uses Wuhan doctor
The SABC immunohistochemical staining kit methods of bio-engineering corporation are obtained, t lymphocyte subset group CD4 in whole blood is determined+、
CD8+Ratio and CD4+/CD8+Ratio；After blood is stood 1h by another pipe at once, 3000r/min centrifugation 10min draw
Supernatant, -20 DEG C of preservations are to be checked.Antibody blocking rate is determined using U.S. IDEXX CSFV ELISA antibody assay kits
（Blocking rate）.
5. statistical analysis
Variance analysis is carried out using the One-Way ANOVA in the Compare Means modules under SAS statistical softwares, and is used
Duncan ' s Multiple range tests carry out group difference significance test, P<0.05 is significant difference.
Application effect of the weanling pig composite probiotics feed additive disclosed by the invention in weanling pig feed, it is main
Including：
（1）Growth performance：
Influence of the lactobacillus acidophilus and bacillus subtilis of the various dose of table 2 to Growth Performance of Weaning Piglets
Note：Shoulder note between colleague, it is not notable without the identical expression difference of letter or lowercase（P ＞ 0.05）, lowercase difference
Represent significant difference（P ＜ 0.05）.Following table is same.
As a result：
1. it follows that the ADG of 3 groups of 2 groups of experiment and experiment is notable（P ＜ 0.05）Higher than control group；The ADG for testing 4 groups is notable
（P ＜ 0.05）Higher than 3 groups of 2 groups of experiment and experiment；Test 2 groups, test 3 groups and the ADFI and F/G and control group phase of 4 groups of experiment
Than difference is not notable（P ＞ 0.05）.
Therefore, individually addition lactobacillus acidophilus or bacillus subtilis can promote the average daily gain of weanling pig, and
After testing 4 groups of compound additions, the influence to ADG becomes apparent from；
2. the ADG for testing 6 groups is notable（P ＜ 0.05）Higher than 7 groups of 4 groups of experiment, 5 groups of experiment and experiment；The ADFI for testing 6 groups is notable
（P ＜ 0.05）Higher than 7 groups of 5 groups of experiment and experiment；The F/G for testing 6 groups is notable（P ＜ 0.05）Less than 7 groups of 5 groups of experiment and experiment；
The equal difference of ADG, ADFI and F/G for testing 5 groups and 7 groups of experiment is not notable（P ＞ 0.05）.
Compared with control group, 5 groups of experiment can have significantly to the growth performance of piglet（P ＜ 0.05）Improve, consider
Feeding effect and cost factor, 6 groups of experiment are optimum addition.
Conclusion：Therefore, lactobacillus acidophilus and bacillus subtilis addition scope are in this probiotics compound additive
0.001~0.01（Concentration is 1 × 10 in diet4 CFU/g~1 × 105CFU/g）.
（2）Physiochemical indice：
1. control group：Basic drawing（Without probiotic additive）；
2. 6 groups are tested：The lactobacillus acidophilus additive of Basic drawing+0.01（Concentration is 1 × 105CFU/g）+ 0.01 withered grass
Bacillus additive（Concentration is 1 × 105CFU/g）；
Influence of the table 3 to physiochemical indice
Note：Colleague's shoulder note lowercase is different to represent significant differenceP<0.05, shoulder note capitalization is different to represent that difference is extremely notableP
<0.01, represent that difference is not notable without shoulder note letterP>0.05.* is representedP<0.01。
Shown by the test data result of table 3：
（1）T lymphocyte subset group is the important indicator for reflecting that whether normal body inner cell immunologic function is, works as CD4+/CD8+Than
When value maintains higher level, illustrate that body is in immune state higher, the CD4 of 6 groups of experiment+/CD8+Ratio is significantly higher than
Control group, therefore, the composite probiotics feed additive can improve pigling immunity.
（2）Compared with control group, lactobacillus acidophilus and bacillus subtilis are added in 6 groups of daily rations of experiment, extremely significantly（P ＜
0.01）Improve the antibody blocking rate of CSFV.Therefore, the composite probiotics feed additive contributes to weanling pig virus
The generation of antibody, so as to improve pigling immunity.
The present invention has carried out security to the feed formula with lactobacillus acidophilus and bacillus subtilis as feed addictive
Experiment, its method is as follows：
（1）Step：
Material and method：Select 28 ages in days, " Du × long × big " piglet 6, clinical health, hair of 7.52 ± 0.47 kg wean
Educate normal, raise in special metabolic cage, 2 weeks experimental periods.Poisoning manifestations and death condition are observed in experimental period at any time.
（2）The piglet basal feed raw material composition of feeding：
Including corn 62.66, dregs of beans 19, whey powder 4.7, fish meal 4, wheat bran 3.45, glucose 3, acidulant 0.3, phosphoric acid hydrogen
Calcium 0.6, stone flour 0.6, salt 0.3, L lysine HCL 0.1, DL- METHIONINE 0.1, threonine 0.15, piglet premix 1,
Lactobacillus acidophilus 0.02（Viable bacteria concentration is 2 × 10 in diet5CFU/g）, bacillus subtilis 0.02（Viable bacteria is dense in diet
Spend is 2 × 105CFU/g）.
As a result：Above-mentioned experiment diet is fed to weanling pig, continuous feeding 14 days has no obvious intoxicating phenomenon, observation period
It is interior without death, wherein one piglet of random postmortem, each main organs show no obvious abnormalities.
（3）Conclusion：
The lactobacillus acidophilus of addition 0.02% in weanling pig daily ration（Concentration is 2 × 10 in diet5CFU/g）With 0.02%
Bacillus subtilis（Concentration is 2 × 10 in diet5CFU/g）Feeding piglet, feeding effect safety.
Specific embodiment
Make a step to the present invention by following examples to illustrate, but not as limitation of the invention.Embodiments of the invention
Described in parts by weight raw material refer to gram, kilogram or ton, can be according to being adjusted the need for produce reality.It is of the invention used
The various raw materials for arriving are for example：Corn, dregs of beans, whey powder, wheat bran, calcium monohydrogen phosphate, stone flour, salt, L lysine HCL,
DL- METHIONINE, threonine, acidulant, premix, lactobacillus acidophilus, bacillus subtilis etc. are externally on sale；It is wherein following
Product is commercially available：
Acidulant refers to Anhui Hua Yang agriculture and animal husbandries Science and Technology Ltd. product；
Whey powder refers to Tianjin ascension feed corporation,Ltd product；
Piglet premix refers to honest premix（Tianjin）Co., Ltd；
Dregs of beans refers to Cofco Group Co., Ltd.'s product；
Lactobacillus acidophilus additive refers to Wuhan Yuancheng Technology Development Co., Ltd.'s product；
Bacillus subtilis bacteria additive refers to Cangzhou City Yi Hong animal health-care products Co., Ltd；
Corn refers to Tianjin corn Centrix Technology Ltd. product；
Fish meal refers to import peru fish meal；
Wheat bran and glucose refer to Tianjin Ding Sheng feed corporation,Ltds product.
Embodiment 1
Weanling pig compound probiotic additive mixed feed raw material is constituted：
Including corn 61.589, dregs of beans 20, whey powder 3.95, fish meal 4, wheat bran 4, glucose 3, acidulant 0.4, calcium monohydrogen phosphate
0.7th, stone flour 0.7, salt 0.3, L lysine HCL 0.1, DL- METHIONINE 0.1, threonine 0.15, piglet premix 1, thermophilic
Lactobacillus lactis 0.01（Concentration is 1 × 10 in diet5CFU/g）, bacillus subtilis 0.001（In diet concentration be 1 ×
104CFU/g）.Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper,
410 mg manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100
Mg VE, 30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
Preparation method：Corn, two kinds of raw materials of dregs of beans are crushed, other raw materials need not be crushed, according to part by weight point
Do not weighed, mixed in multifunctional mini-mixer.
Embodiment 2
Weanling pig compound probiotic additive mixed feed raw material is constituted：
Including corn 62.589, dregs of beans 19, whey powder 3.95, fish meal 4.5, wheat bran 4, glucose 2.5, acidulant 0.4, phosphoric acid
Hydrogen calcium 0.7, stone flour 0.7, salt 0.3, L lysine HCL 0.1, DL- METHIONINE 0.1, threonine 0.15, piglet premix
1st, lactobacillus acidophilus 0.001（Concentration is 1 × 10 in diet4CFU/g）, bacillus subtilis 0.01（In diet concentration be 1 ×
105CFU/g）.
Wherein described piglet premix refers to contain in every kilogram of premix：14,600 mg iron, 630 mg copper, 410
Mg manganese, 10,400 mg zinc, 15,000 μ g iodine, 17,000 μ g selenium, 227,600 IU VA, 22,800 IU VD3, 1,100 mg
VE, 30 mg VK3, 700 mg VB2, 200 mg VB6, 1,340 mg pantothenic acid, 2,300 mg nicotinic acid, 8 mg biotins.
Preparation method：Corn, two kinds of raw materials of dregs of beans are crushed, other raw materials need not be crushed, according to part by weight point
Do not weighed, mixed in multifunctional mini-mixer.
Embodiment 3
A kind of weanling pig compound probiotic additive mixed feed is made up of the raw material of following portions by weight：
Including corn 63.08, dregs of beans 18.5, whey powder 3.95, fish meal 4, wheat bran 4.5, glucose 2.5, acidulant 0.4, phosphorus
Sour hydrogen calcium 0.7, stone flour 0.7, salt 0.3, L lysine HCL 0.1, DL- METHIONINE 0.1, threonine 0.15, piglet premix
Material 1, lactobacillus acidophilus 0.01（Concentration is 1 × 10 in diet5CFU/g）, bacillus subtilis 0.01（In diet concentration be 1 ×
105CFU/g）.
Preparation method：Corn, two kinds of raw materials of dregs of beans are crushed, other raw materials need not be crushed, according to part by weight point
Do not weighed, mixed in multifunctional mini-mixer.
Embodiment 4
The method that weanling pig is fed using the mixed feed of weanling pig：
This series formula is applicable weanling pig, and working life is 30 ~ 45 days, according to circumstances allows piglet free choice feeding, it is necessary to carry
For sufficient drinking-water, other are carried out by normal feeding and management.
Embodiment 5
Comparative test
Conventional pig starter feed：
Method：Corn-soybean meal Piglet Diets are prepared, without lactobacillus acidophilus and bacillus subtilis.Selection 6 first 7.28 ±
0.54 kg piglets, 14 days experimental periods.6 piglets are taken a blood sample after off-test.
Step：After off-test, piglet adopts two pipe blood by vena cava anterior, and after blood sampling, a pipe is using Wuhan doctor get Sheng
The SABC immunohistochemical staining kit methods of thing engineering company, determine t lymphocyte subset group CD4 in whole blood+、CD8+'s
Ratio and CD4+/CD8+Ratio；After blood is stood 1h by another pipe at once, 3000r/min centrifugation 10min draw supernatant
Liquid, -20 DEG C of preservations are to be checked, and the antibody blocking of CSFV is determined using U.S. IDEXX CSFV ELISA antibody assay kits
Rate.
As a result：CD4 in whole blood+、CD8+And CD4+/CD8+Respectively 28.51%, 27.92% and 1.02, CSFV in serum
Antibody blocking rate is 56.34%.
Feed after lactobacillus acidophilus and bacillus subtilis is added in daily ration：
Method：Corn-soybean meal piglet diet is prepared, 0.01% lactobacillus acidophilus and bacillus subtilis 0.01% is added.Selection 6
First 7.31 ± 0.48kg piglets, 14 days experimental periods.6 piglets are taken a blood sample after off-test.
Step：After off-test, piglet adopts two pipe blood by vena cava anterior, and after blood sampling, a pipe is using Wuhan doctor get Sheng
The SABC immunohistochemical staining kit methods of thing engineering company, determine t lymphocyte subset group CD4 in whole blood+、CD8+'s
Ratio and CD4+/CD8+Ratio；After blood is stood 1h by another pipe at once, 3000r/min centrifugation 10min draw supernatant
Liquid, -20 DEG C of preservations are to be checked, and the antibody blocking of CSFV is determined using U.S. IDEXX CSFV ELISA antibody assay kits
Rate.
As a result：CD4 in whole blood+、CD8+And CD4+/CD8+Respectively 29.74%, 24.83% and 1.20, CSFV in serum
Antibody blocking rate is 78.79%.
Conclusion：Lactobacillus acidophilus and bacillus subtilis are added in daily ration can significantly improve CD4 in whole blood+/CD8+Ratio
Value, significantly improves the antibody blocking rate of change of serum C SFV.Illustrated by comparative test：Lactobacillus acidophilus and withered grass bud are added in daily ration
Spore bacillus can adjust pigling immunity.
Claims (4)
1. a kind of weanling pig composite probiotics feed additive, it is characterised in that in feedstuff using lactobacillus acidophilus and
Bacillus subtilis bacteria additive, carries out reasonable preparation daily ration, is made up of the raw material of following portions by weight：
Corn 58-65 dregs of beans 16-24 whey powders 2-5
Fish meal 4-8 wheat bran 1-5 glucose 1-3
Acidulant 0.1-0.5 calcium monohydrogen phosphate 0.5-1.5 stone flours 0.5-1.2
Salt 0.1-0.6 L FE-5 0.1-0.8 DL- METHIONINEs 0.1-0.7
Threonine 0.1-0.7 piglet premixes 1-4
Lactobacillus acidophilus additive 0.001-0.01（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g, in diet concentration be 1 ×
104~1×105CFU/g）
Bacillus subtilis bacteria additive 0.001-0.01（Viable bacteria content is 1 × 10 in freeze-dried powder9CFU/g, in diet concentration be 1 ×
104~1×105CFU/g）.
2. the weanling pig composite probiotics feed additive described in claim 1, wherein lactobacillus acidophilus additive and withered grass
Viable bacteria content is 1 × 10 in the lyophilized bacterium powder of bacillus additive9CFU/g, the content of two kinds of viable bacterias is equal in diet prescription
It is 1 × 105CFU/g。
3. weanling pig composite probiotics feed additive described in claim 1 Growth Performance of Weaning Piglets and is being exempted from as improving
Application in epidemic disease power.
4. the application described in claim 2, wherein described raising Growth Performance of Weaning Piglets and immunity are referred to：Wean is young
The average daily gain of pig（ADG）, average daily gain（ADFI）And feed-weight ratio（F/G）, t lymphocyte subset group CD4 in whole blood+、
CD8+Ratio and CD4+/CD8+Ratio, serum in CSFV（CSFV）Antibody blocking rate.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107348189A (en) * | 2017-07-20 | 2017-11-17 | 贵阳富源饲料有限公司 | A kind of sucking pig mixed feed |
| CN108967693A (en) * | 2018-07-09 | 2018-12-11 | 中国农业科学院北京畜牧兽医研究所 | A kind of weanling pig mixed feed and its application containing compound probiotic |
| CN111134235A (en) * | 2020-03-05 | 2020-05-12 | 润盈生物工程（上海）有限公司 | Lactobacillus acidophilus microbial agent capable of preventing African swine fever and application thereof |
| CN111357710A (en) * | 2018-12-26 | 2020-07-03 | 河南银发牧业有限公司 | Feeding method of IUGR weaned piglets |
| CN113287680A (en) * | 2021-05-28 | 2021-08-24 | 新疆天康饲料有限公司 | Winter low-temperature anti-freezing fermented feed wet material and preparation method and application thereof |
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| CN113287680A (en) * | 2021-05-28 | 2021-08-24 | 新疆天康饲料有限公司 | Winter low-temperature anti-freezing fermented feed wet material and preparation method and application thereof |
| CN113287680B (en) * | 2021-05-28 | 2023-09-22 | 新疆天康饲料有限公司 | Winter low-temperature anti-freezing fermented feed wet material and preparation method and application thereof |
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