CN102893855A - Production method of environment-friendly economic cotton hybrid - Google Patents
Production method of environment-friendly economic cotton hybrid Download PDFInfo
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- CN102893855A CN102893855A CN2012104084153A CN201210408415A CN102893855A CN 102893855 A CN102893855 A CN 102893855A CN 2012104084153 A CN2012104084153 A CN 2012104084153A CN 201210408415 A CN201210408415 A CN 201210408415A CN 102893855 A CN102893855 A CN 102893855A
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Abstract
The invention discloses a production method of an environment-friendly economic cotton hybrid. As the production method adopts a special parent, the hybrid can be subjected to forge identification indoors during a main leaf period, so that the rapidness and the accuracy of the identification are improved, the purity of seeds is guaranteed, the stability of a seed market is ensured, the seed quality is guaranteed, and the phenomenon of forging and faking is effectively attacked. The production method can greatly reduce acid content during a process of removing woolen by sulfuric acid before sowing, reduce the damage to the seeds by the acid, guarantee the integrity of the seeds and protect environment, is beneficial to the growth of the seeds, is simple and practical, can be used for identifying the purity of the hybrid well for multiple times, avoids the phenomenon of forging and faking well, and protects the benefits of cotton planters.
Description
Technical field
The invention belongs to the cotton planting field, relate in particular to a kind of method of producing environmental protection and economy type cotton hybrid kind.
Background technology
The high yield improved seeds are volume increase bases of crop, each crop large tracts of land volume increase all is the result of good replacement kind spread, cultivating good replacement kind is the direction that each large agricultural country of the world endeavours to study all the time, and it is exactly a good example that hybrid cotton is promoted.But pay attention to usually causing the grower to underestimate mistakenly the yield potential of improved seeds not for existing problems in the improved seeds popularization, delay and make improved seeds promote time, even hesitate to move forward.For crossbreed, purity of hybrid height and yield potential size are mutually to echo in the production, and good Hybrid purity descends, and extremely affects the performance of kind yield potential even causes the underproduction.Cotton hybrid production of hybrid seeds majority is the artificial emasculation pollination, and whether thoroughly artificial emasculation directly affects the quality of seed, and artificial emasculation not is a major reason of impact hybridization cotton seed purity thoroughly or leak and spent the maternal selfing Cheng Ling that hero causes; More have or not good production of hybrid seeds family to pay seed inferior.And for cotton seed purity Morphological Identification, almost be that naked eyes can not be distinguished, for the strain Purity of cotton seed, mend the fold after the sheep is lost often late, do not reach the set goal.
Summary of the invention
Purpose of the present invention provides a kind of method of producing environmental protection and economy type cotton hybrid kind, is intended to solve existing Purity to cotton seed, the problem that falls flat.
The object of the present invention is to provide a kind of method of producing environmental protection and economy type cotton hybrid kind, said method comprising the steps of:
Non-transgenic light seed female parent is cultivated screening, and the light seed female parent of high-combining ability is cultivated in screening;
Choose high-combining ability transgenic pest-resistant material and do male parent, with high-purity light seed hybridization of female parent, obtain transformation transgenic pest-resistant crossbreed;
Crossbreed for high yield carries out Purity.
Further, described female parent is cultivated screening, guarantees that maternal high-purity method is:
Seed selection non-transgenic light unginned cotton flower parent is maternal, directly the hair seed that mixes can be rejected after cotton ginning.In the field planting management process, use the kanamycin evaluation and screening seedling stage, the transgenosis hybrid strain is pulled out, guarantee maternal purity.
Further, the described male parent of choosing, with the high-purity hybridization of female parent, the method for obtaining crossbreed is:
Seed selection high-combining ability transgene cotton is made male parent, hybridizes assembly with light seed female parent, obtains a collection of transgenosis photon cotton crossbreed F
1By randomized block experiment design colored crossbreed F that plantation is distributed cotton
1, require to test according to People's Republic of China's agricultural industry criteria (NY/T1302-2007) " variety of crops experimental technique rules-cotton ", therefrom select high yield, high-quality and many anti-F
1Strong advantage combination.
Further, described crossbreed for high yield carries out the Purity method and is;
The first step is to the F of results
1Seed carries out Morphological Identification, rejects the Westerner and keeps the light seeds, the seed that is on the female parent to be tied with the seed that guarantees results; Second step is to above-mentioned smooth seed F
1The sample seed sprays or smears kanamycin at indoor culture to leaf period, identifies non-transgenic male parent pollination ratio in the sample; Identify by secondary, crossbreed F guarantees to be up to the standards
1It is highly purified seed.
Further, described method adopts special parent that crossbreed is carried out Purity, identifies the maternal fraud of crossbreed doping.
Further, sulfuric acid lint process had been protected environment with the acid amount before described method had greatly reduced sowing.
The present invention is a kind of method of production environmental protection and economy type cotton hybrid kind, the method is utilized special parent, improve the accuracy to Hybrid seed purity test, can whether add female parent to crossbreed in indoor seedling stage and make evaluation, well stopped fake and forged phenomenon; Protected environment, sulfuric acid lint process greatly reduces with the acid amount before the sowing, has alleviated sulfuric acid lint process and salt acid vapour, the concentrated sulfuric acid, dilute sulfuric acid, foamed acid etc. to the pollution of environment; Save cost; Reduced the seed mechanical damage, the growing way of emerging is good, and the seed suction is fast,, be beneficial to and early send out strong sprout; Improved the seed disease resistance.
Description of drawings
Fig. 1 is the method realization flow figure of the production environmental protection and economy type cotton hybrid kind that provides of the embodiment of the invention.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, is not intended to limit the present invention.
The embodiment of the invention provides a kind of method of producing environmental protection and economy type cotton hybrid kind, and the method may further comprise the steps:
Female parent is cultivated screening, guarantee maternal high-purity;
Choose male parent, with the high-purity hybridization of female parent, obtain crossbreed;
Crossbreed for high yield carries out Purity.
As a prioritization scheme of the embodiment of the invention, method adopts special parent that crossbreed is carried out Purity, identifies the maternal fraud of crossbreed doping.
As a prioritization scheme of the embodiment of the invention, sulfuric acid lint process had been protected environment with the acid amount before method had greatly reduced sowing.
Referring to accompanying drawing 1, the method for the embodiment of the invention being produced environmental protection and economy type cotton hybrid kind is described in further detail.
As shown in Figure 1, the method for production environmental protection and economy type cotton hybrid kind of the present invention;
Step S101: seed selection light seed is maternal, cultivates screening; Seed selection high-combining ability non-transgenic photon cotton parent is maternal.In the field planting management process, use in early days the kanamycin evaluation and screening, the transgenic pest-resistant hybrid strain is pulled out, guarantee maternal purity.
Step S102: seed selection high-combining ability transgenic pest-resistant material is done male parent, hybridizes assembly with light seed female parent, obtains the pest-resistant cotton crossbreed F of a collection of transgenosis light seed
1By randomized block experiment design colored Hybrid F1 that plantation is distributed cotton, require to test according to People's Republic of China's agricultural industry criteria (NY/T1302-2007) " variety of crops experimental technique rules one cotton ".Therefrom select high yield, high-quality and many anti-F
1Strong advantage combination.
Step S103: Purity; The first step is to the F of results
1Seed carries out Morphological Identification, rejects the Westerner and keeps the light seeds, the seed that is on the female parent to be tied with the seed that guarantees results.Second step is to above-mentioned photon F
1The sample seed sprays or smears kanamycin at indoor culture to leaf period, identifies non-transgenic male parent pollination ratio in the sample.Identify by secondary, crossbreed F guarantees to be up to the standards
1It is highly purified transgenic pest-resistant seed.
The method of a kind of production economy environment-friendly type cotton crossbreed of the present invention mainly may further comprise the steps:
The first step, seed selection female parent are cultivated screening; Seed selection high-combining ability non-transgenic light unginned cotton flower parent is maternal, in the field planting management process, uses in early days the kanamycin evaluation and screening, and the transgenic Bt hybrid strain is pulled out, and guarantees maternal purity.
Second step, seed selection high-combining ability transgenic pest-resistant material are done male parent, hybridize assembly with light seed female parent, obtain the pest-resistant cotton crossbreed F of a collection of transgenosis photon
1By randomized block experiment design colored Hybrid F1 that plantation is distributed cotton, require to test according to People's Republic of China's agricultural industry criteria (NY/T1302-2007) " variety of crops experimental technique rules-cotton ".Therefrom select high yield, high-quality and many anti-F
1Strong advantage combination.
The 3rd step, Purity; The first step is to the F of results
1Seed carries out Morphological Identification, rejects the Westerner and keeps the light seeds, the seed that is on the female parent to be tied with the seed that guarantees results.Second step is to above-mentioned photon F
1The sample seed is in indoor seedling spraying or smear kanamycin, identifies non-transgenic male parent pollination ratio in the sample.Identify by secondary, crossbreed F guarantees to be up to the standards
1It is highly purified seed.
The method of production environmental protection and economy type cotton hybrid kind of the present invention, the method is utilized special parent, improve the accuracy to Hybrid seed purity test, can whether add female parent to crossbreed and make evaluation leaf period is indoor, well stopped fake and forged phenomenon; Protected environment, sulfuric acid lint process greatly reduces with the acid amount before the sowing, has alleviated sulfuric acid lint process and salt acid vapour, the concentrated sulfuric acid, dilute sulfuric acid, foamed acid etc. to the pollution of environment; Save cost; Reduced the seed mechanical damage, the growing way of emerging is good, and the seed suction is fast,, be beneficial to and early send out strong sprout; Improved the seed disease resistance.
The above only is preferred embodiment of the present invention, not in order to limiting the present invention, all any modifications of doing within the spirit and principles in the present invention, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. a method of producing environmental protection and economy type cotton hybrid kind is characterized in that, said method comprising the steps of:
Female parent is cultivated screening, guarantee maternal high-purity;
Choose male parent, with the high-purity hybridization of female parent, obtain crossbreed;
Crossbreed for high yield carries out Purity.
2. the method for production environmental protection and economy type cotton hybrid kind as claimed in claim 1 is characterized in that, described female parent is cultivated screening, guarantees that maternal high-purity method is:
Seed selection non-transgenic light unginned cotton flower parent is maternal, directly the hair seed that mixes can be rejected after cotton ginning.In the field planting management process, use the kanamycin evaluation and screening seedling stage, the transgenosis hybrid strain is pulled out, guarantee maternal purity.
3. the method for production environmental protection and economy type cotton hybrid kind as claimed in claim 1 is characterized in that, described seed selection male parent, and with the high-purity hybridization of female parent, the method for obtaining crossbreed is:
Seed selection high-combining ability transgene cotton is made male parent, hybridizes assembly with light seed female parent, obtains a collection of transgenosis photon cotton crossbreed F
1By randomized block experiment design colored crossbreed F that plantation is distributed cotton
1, require to test according to People's Republic of China's agricultural industry criteria " variety of crops experimental technique rules one cotton ", therefrom select high yield, high-quality and many anti-F
1Strong advantage combination.
4. the method for production environmental protection and economy type cotton hybrid kind as claimed in claim 1 is characterized in that, described crossbreed for high yield carries out the Purity method and is;
The first step is to the F of results
1Seed carries out Morphological Identification, rejects the Westerner and keeps the light seeds, the seed that is on the female parent to be tied with the seed that guarantees results; Second step is to above-mentioned smooth seed F
1The sample seed sprays or smears kanamycin at indoor culture to leaf period, identifies non-transgenic male parent pollination ratio in the sample; Identify by secondary, crossbreed F guarantees to be up to the standards
1It is highly purified seed.
5. the method for production environmental protection and economy type cotton hybrid kind as claimed in claim 1 is characterized in that, described method adopts special parent that crossbreed is carried out Purity, identifies the maternal fraud of crossbreed doping.
6. the method for production environmental protection and economy type cotton hybrid kind as claimed in claim 1 is characterized in that, sulfuric acid lint process had been protected environment with the acid amount before described method had greatly reduced sowing.
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| CN201210408415.3A CN102893855B (en) | 2012-10-14 | 2012-10-14 | Production method of environment-friendly economic cotton hybrid |
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| CN201210408415.3A CN102893855B (en) | 2012-10-14 | 2012-10-14 | Production method of environment-friendly economic cotton hybrid |
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| CN102893855A true CN102893855A (en) | 2013-01-30 |
| CN102893855B CN102893855B (en) | 2014-03-19 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113498734A (en) * | 2021-06-29 | 2021-10-15 | 辽宁省经济作物研究所 | Non-transgenic cotton breeding method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1237329A (en) * | 1999-05-28 | 1999-12-08 | 孙长法 | Breeding method of bare seed cotton variety |
| CN1823579A (en) * | 2005-02-23 | 2006-08-30 | 朱安灿 | Transgene crop pollen competitive agent and method for cotten seed preparation |
| CN101564003A (en) * | 2009-04-02 | 2009-10-28 | 王留明 | Method for preparing and identifying cotton crossbreed |
| EP2258856A1 (en) * | 1998-02-20 | 2010-12-08 | Syngenta Limited | Hybrid seed production |
| CN102613067A (en) * | 2012-02-29 | 2012-08-01 | 河南省农业科学院 | Cotton hybrid breeding method utilizing double labeling method |
-
2012
- 2012-10-14 CN CN201210408415.3A patent/CN102893855B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2258856A1 (en) * | 1998-02-20 | 2010-12-08 | Syngenta Limited | Hybrid seed production |
| CN1237329A (en) * | 1999-05-28 | 1999-12-08 | 孙长法 | Breeding method of bare seed cotton variety |
| CN1823579A (en) * | 2005-02-23 | 2006-08-30 | 朱安灿 | Transgene crop pollen competitive agent and method for cotten seed preparation |
| CN101564003A (en) * | 2009-04-02 | 2009-10-28 | 王留明 | Method for preparing and identifying cotton crossbreed |
| CN102613067A (en) * | 2012-02-29 | 2012-08-01 | 河南省农业科学院 | Cotton hybrid breeding method utilizing double labeling method |
Non-Patent Citations (3)
| Title |
|---|
| K.R.KRANTHI: "Temporal and intra-plant variability of Cry1Ac expression in Bt-cotton and its influence on the survival of the cotton bollworm, Helicoverpa armigera (Hübner) (Noctuidae: Lepidoptera)", 《CURRENT SCIENCE》 * |
| 叶鹏盛等: "利用卡那霉素间接鉴定转基因抗虫棉的研究", 《四川农业大学学报》 * |
| 郭承君等: "标记转基因杂交棉育种研究进展初报", 《中国棉花学会2007年年会论文汇编》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113498734A (en) * | 2021-06-29 | 2021-10-15 | 辽宁省经济作物研究所 | Non-transgenic cotton breeding method |
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