CN102893855B - Production method of environment-friendly economic cotton hybrid - Google Patents
Production method of environment-friendly economic cotton hybrid Download PDFInfo
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- CN102893855B CN102893855B CN201210408415.3A CN201210408415A CN102893855B CN 102893855 B CN102893855 B CN 102893855B CN 201210408415 A CN201210408415 A CN 201210408415A CN 102893855 B CN102893855 B CN 102893855B
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Abstract
The invention discloses a production method of an environment-friendly economic cotton hybrid. As the production method adopts a special parent, the hybrid can be subjected to forge identification indoors during a main leaf period, so that the rapidness and the accuracy of the identification are improved, the purity of seeds is guaranteed, the stability of a seed market is ensured, the seed quality is guaranteed, and the phenomenon of forging and faking is effectively attacked. The production method can greatly reduce acid content during a process of removing woolen by sulfuric acid before sowing, reduce the damage to the seeds by the acid, guarantee the integrity of the seeds and protect environment, is beneficial to the growth of the seeds, is simple and practical, can be used for identifying the purity of the hybrid well for multiple times, avoids the phenomenon of forging and faking well, and protects the benefits of cotton planters.
Description
Technical field
The invention belongs to cotton planting field, relate in particular to a kind of method of producing environmental protection and economy type cotton hybrid kind.
Background technology
High yield improved seeds are volume increase bases of crop, each crop large area volume increase is all the result of good replacement kind spread, cultivating good replacement kind is the direction that each large agricultural country of the world endeavours research all the time, and it is exactly a good example that hybrid cotton is promoted.But in promoting for improved seeds, existing problems are paid attention to not, usually causing grower to underestimate mistakenly the yield potential of improved seeds, and make improved seeds promote delay in time, even hesitate to move forward.For crossbreed, in production, purity of hybrid height and yield potential size are mutually to echo, and good Hybrid purity declines, and the performance that extremely affects kind yield potential even causes the underproduction.Cotton hybrid production of hybrid seeds majority is artificial emasculation pollination, and whether thoroughly artificial emasculation directly affects the quality of seed, and artificial emasculation not thoroughly or leak and spent the maternal selfing Cheng Ling that hero causes, is a major reason of impact hybridization cotton seed purity; More have or not good production of hybrid seeds family to pay seed inferior.And for cotton seed purity Morphological Identification, be almost that naked eyes can not be distinguished, for the strain Purity of cotton seed, mend the fold after the sheep is lost often late, do not reach the set goal.
Summary of the invention
Object of the present invention provides a kind of method of producing environmental protection and economy type cotton hybrid kind, is intended to solve the existing Purity to cotton seed, the problem falling flat.
The object of the present invention is to provide a kind of method of producing environmental protection and economy type cotton hybrid kind, said method comprising the steps of:
Non-transgenic light seed female parent is cultivated to screening, and the light seed female parent of high-combining ability is cultivated in screening;
Choose high-combining ability transgenic pest-resistant material and do male parent, with high-purity light seed hybridization of female parent, obtain transformation transgenic pest-resistant crossbreed;
Crossbreed for high yield carries out Purity.
Further, described female parent is cultivated to screening, guarantees that maternal high-purity method is:
Seed selection non-transgenic light unginned cotton flower parent is maternal, after cotton ginning, directly the hair seed mixing can be rejected.In field planting management process, use kanamycin evaluation and screening seedling stage, transgenosis hybrid strain is pulled out, guarantee maternal purity.
Further, described in choose male parent, with high-purity hybridization of female parent, the method for obtaining crossbreed is:
Seed selection high-combining ability transgene cotton is made male parent, hybridizes assembly with light seed female parent, obtains a collection of transgenosis photon cotton crossbreed F
1; By randomized block experiment design cotton crossbreed F that plantation is joined
1according to People's Republic of China's agricultural industry criteria (NY/T1302-2007) < < variety of crops experimental technique rules-cotton > >, require to test, therefrom select high yield, high-quality and how anti-F
1strong advantage combination.
Further, the described crossbreed for high yield carries out Purity method and is;
The F of the first step to results
1seed carries out Morphological Identification, rejects Westerner and retains light seeds, with the seed that guarantees that the seed of results is on female parent to be tied; Second step is to above-mentioned smooth seed F
1sample seed sprays or smears kanamycin at indoor culture to leaf period, identifies non-transgenic male parent pollination ratio in sample; By secondary, identify, crossbreed F guarantees to be up to the standards
1it is highly purified seed.
Further, described method adopts special parent to carry out Purity to crossbreed, identifies the crossbreed maternal fraud of adulterating.
Further, before described method has greatly reduced sowing, sulfuric acid lint process is measured with acid, has protected environment.
The present invention is a kind of method of production environmental protection and economy type cotton hybrid kind, the method is utilized special parent, improve the accuracy to Hybrid seed purity test, in indoor seedling stage, can whether add female parent to crossbreed and make evaluation, well stopped fake and forged phenomenon; Protected environment, before sowing, sulfuric acid lint process greatly reduces by acid amount, has alleviated the pollution to environment such as sulfuric acid lint process and salt acid vapour, the concentrated sulfuric acid, dilute sulfuric acid, foamed acid; Cost-saving; Reduced seed mechanical damage, the growing way of emerging is good, and seed water suction is fast,, be beneficial to and early send out strong sprout; Improved seed disease resistance.
Accompanying drawing explanation
Fig. 1 is the method realization flow figure of the production environmental protection and economy type cotton hybrid kind that provides of the embodiment of the present invention.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
The embodiment of the present invention provides a kind of method of producing environmental protection and economy type cotton hybrid kind, and the method comprises the following steps:
Female parent is cultivated to screening, guarantee maternal high-purity;
Choose male parent, with high-purity hybridization of female parent, obtain crossbreed;
Crossbreed for high yield carries out Purity.
As a prioritization scheme of the embodiment of the present invention, method adopts special parent to carry out Purity to crossbreed, identifies the crossbreed maternal fraud of adulterating.
As a prioritization scheme of the embodiment of the present invention, before method has greatly reduced sowing, sulfuric acid lint process is measured with acid, has protected environment.
Referring to accompanying drawing 1, the method for the embodiment of the present invention being produced to environmental protection and economy type cotton hybrid kind is described in further detail.
As shown in Figure 1, the method for production environmental protection and economy type cotton hybrid kind of the present invention;
Step S101: seed selection light seed is maternal, cultivates screening; Seed selection high-combining ability non-transgenic photon cotton parent is maternal.In field planting management process, use in early days kanamycin evaluation and screening, transgenic pest-resistant hybrid strain is pulled out, guarantee maternal purity.
Step S102: seed selection high-combining ability transgenic pest-resistant material is done male parent, hybridizes assembly with light seed female parent, obtains the pest-resistant cotton crossbreed F of a collection of transgenosis light seed
1.By randomized block experiment design colored Hybrid F1 that plantation is distributed cotton, according to People's Republic of China's agricultural industry criteria (NY/T1302-2007) < < variety of crops experimental technique rules one cotton > >, require to test.Therefrom select high yield, high-quality and how anti-F
1strong advantage combination.
Step S103: Purity; The F of the first step to results
1seed carries out Morphological Identification, rejects Westerner and retains light seeds, with the seed that guarantees that the seed of results is on female parent to be tied.Second step is to above-mentioned photon F
1sample seed sprays or smears kanamycin at indoor culture to leaf period, identifies non-transgenic male parent pollination ratio in sample.By secondary, identify, crossbreed F guarantees to be up to the standards
1it is highly purified transgenic pest-resistant seed.
The method of a kind of production economy environment-friendly type cotton crossbreed of the present invention, mainly comprises the following steps:
The first step, seed selection female parent, cultivate screening; Seed selection high-combining ability non-transgenic light unginned cotton flower parent is maternal, in field planting management process, uses in early days kanamycin evaluation and screening, and transgenic Bt hybrid strain is pulled out, and guarantees maternal purity.
Second step, seed selection high-combining ability transgenic pest-resistant material are done male parent, hybridize assembly with light seed female parent, obtain the pest-resistant cotton crossbreed F of a collection of transgenosis photon
1.By randomized block experiment design colored Hybrid F1 that plantation is distributed cotton, according to People's Republic of China's agricultural industry criteria (NY/T1302-2007) < < variety of crops experimental technique rules-cotton > >, require to test.Therefrom select high yield, high-quality and how anti-F
1strong advantage combination.
The 3rd step, Purity; The F of the first step to results
1seed carries out Morphological Identification, rejects Westerner and retains light seeds, with the seed that guarantees that the seed of results is on female parent to be tied.Second step is to above-mentioned photon F
1sample seed is in indoor seedling spraying or smear kanamycin, identifies non-transgenic male parent pollination ratio in sample.By secondary, identify, crossbreed F guarantees to be up to the standards
1it is highly purified seed.
The method of production environmental protection and economy type cotton hybrid kind of the present invention, the method is utilized special parent, improve the accuracy to Hybrid seed purity test, can whether add female parent to crossbreed and make evaluation leaf period is indoor, well stopped fake and forged phenomenon; Protected environment, before sowing, sulfuric acid lint process greatly reduces by acid amount, has alleviated the pollution to environment such as sulfuric acid lint process and salt acid vapour, the concentrated sulfuric acid, dilute sulfuric acid, foamed acid; Cost-saving; Reduced seed mechanical damage, the growing way of emerging is good, and seed water suction is fast,, be beneficial to and early send out strong sprout; Improved seed disease resistance.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (1)
1. a method of producing environmental protection and economy type cotton hybrid kind, is characterized in that, said method comprising the steps of:
Female parent is cultivated to screening, guarantee maternal high-purity;
Choose male parent, with high-purity hybridization of female parent, obtain crossbreed;
Crossbreed for high yield carries out Purity;
Described female parent is cultivated to screening, guarantees that maternal high-purity method is:
Seed selection non-transgenic light unginned cotton flower parent is maternal, after cotton ginning, directly the hair seed mixing can be rejected; In field planting management process, use kanamycin evaluation and screening seedling stage, transgenosis hybrid strain is pulled out, guarantee maternal purity;
Described seed selection male parent, with high-purity hybridization of female parent, the method for obtaining crossbreed is:
Seed selection high-combining ability transgene cotton is made male parent, hybridizes assembly with light seed female parent, obtains a collection of transgenosis photon cotton crossbreed F1; By randomized block experiment design colored Hybrid F1 that plantation is distributed cotton, according to the agricultural industry criteria < < of People's Republic of China (PRC) variety of crops experimental technique rules one cotton > >, require to test, therefrom select high yield, high-quality and the how anti-strong advantage combination of F1;
The method of described production environmental protection and economy type cotton hybrid kind adopts special parent to carry out Purity to crossbreed, identifies the crossbreed maternal fraud of adulterating;
The described crossbreed for high yield carries out Purity method;
The first step is carried out Morphological Identification to the F1 seed of results, rejects Westerner and retains light seeds, with the seed that guarantees that the seed of results is on female parent to be tied; Second step sprays or smears kanamycin at indoor culture to leaf period to above-mentioned smooth seed F1 sample seed, identifies non-transgenic male parent pollination ratio in sample; By secondary, identify, the Hybrid F1 of guaranteeing to be up to the standards is highly purified seed.
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| CN201210408415.3A CN102893855B (en) | 2012-10-14 | 2012-10-14 | Production method of environment-friendly economic cotton hybrid |
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| CN201210408415.3A CN102893855B (en) | 2012-10-14 | 2012-10-14 | Production method of environment-friendly economic cotton hybrid |
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| CN102893855A CN102893855A (en) | 2013-01-30 |
| CN102893855B true CN102893855B (en) | 2014-03-19 |
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| CN113498734A (en) * | 2021-06-29 | 2021-10-15 | 辽宁省经济作物研究所 | Non-transgenic cotton breeding method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1237329A (en) * | 1999-05-28 | 1999-12-08 | 孙长法 | Breeding method of bare seed cotton variety |
| CN1823579A (en) * | 2005-02-23 | 2006-08-30 | 朱安灿 | Transgene crop pollen competitive agent and method for cotten seed preparation |
| CN101564003A (en) * | 2009-04-02 | 2009-10-28 | 王留明 | Method for preparing and identifying cotton crossbreed |
| EP2258856A1 (en) * | 1998-02-20 | 2010-12-08 | Syngenta Limited | Hybrid seed production |
| CN102613067A (en) * | 2012-02-29 | 2012-08-01 | 河南省农业科学院 | Cotton hybrid breeding method utilizing double labeling method |
-
2012
- 2012-10-14 CN CN201210408415.3A patent/CN102893855B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2258856A1 (en) * | 1998-02-20 | 2010-12-08 | Syngenta Limited | Hybrid seed production |
| CN1237329A (en) * | 1999-05-28 | 1999-12-08 | 孙长法 | Breeding method of bare seed cotton variety |
| CN1823579A (en) * | 2005-02-23 | 2006-08-30 | 朱安灿 | Transgene crop pollen competitive agent and method for cotten seed preparation |
| CN101564003A (en) * | 2009-04-02 | 2009-10-28 | 王留明 | Method for preparing and identifying cotton crossbreed |
| CN102613067A (en) * | 2012-02-29 | 2012-08-01 | 河南省农业科学院 | Cotton hybrid breeding method utilizing double labeling method |
Non-Patent Citations (2)
| Title |
|---|
| 标记转基因杂交棉育种研究进展初报;郭承君等;《中国棉花学会2007年年会论文汇编》;20071231;第252-253页，尤其是第252页第2段、第5段 * |
| 郭承君等.标记转基因杂交棉育种研究进展初报.《中国棉花学会2007年年会论文汇编》.2007,第252-253页，尤其是第252页第2段，第5段. |
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