CN1138467C - Method for preparing disease-resistant nuclear-sterile dual-purpose line and hybrid of cotton - Google Patents

Abstract

The present invention provides a production method of a disease resistant double-function genic male sterile line GA10 of cotton. The method adopts A1 resistant sterile plants and CRI (Cotton Research Institute) No. 12 for hybridization, and then the A1 resistant sterile plants are adopted as a female parent for back cross; sterile plants produced after the back cross are hybridized with a high-quality cotton strain B17, obtained descendants are continuously mixed twice for sib cross, and then sib cross between opposite plants is carried out; the sterile line is bred by screening disease resistance by artificial bacterium inoculation and screening fiber quality. The present invention also provides a production method of a high-advantage genic male sterile hybrid VH3 of cotton with insect resistance, high quality and disease resistance, which utilizes the female parent of the sterile plants of the disease resistant double-function genic male sterile line GA10 of cotton, and the male parent of a strain HB of insect resistant cotton, and the seeds of the F1 generation of insect resistant hybrid VH3F1 are produced by hybridization by artificial supplementary pollination and adopting the method for using the genic male sterile line for both functions.

Description

The production method of the sterile hybrid of the disease-resistant nuclear of bt-cotton

Technical field:

What the present invention relates to is a kind of crossbreeding field, the special and sterile hybrid VH of the disease-resistant nuclear of bt-cotton ₃Production method relevant.

Background technology:

Cotton bollworm, pink bollworm etc. are increased the weight of day by day to the harm of output of cotton and quality, and the control efficiency of chemical agent reduces year by year, and the pollution of environment is on the rise, and therefore, the seed selection pest-resistant cultivar is best solution.Use in the production large tracts of land for cotton No. 15 at present domestic No. 29, pest-resistant hybrid middle cotton having cultivated 2 artificial emasculations and Shandong, and the shortcoming of these two pest-resistant hybrids is that artificial emasculation produces F ₁Too high for seed costs, F ₂For the not pest-resistant plant that will isolate 25% again the resistance to insects of colony is descended, and then influence yield and quality.

Summary of the invention:

In view of above reason, the objective of the invention is provides a kind of production cost to reduce significantly in order to overcome above deficiency, the output height, and increasing degree is big, and fiber quality is good, and pest-resistant disease resistance is good, the sterile hybrid VH of the disease-resistant nuclear of adaptable bt-cotton ₃Production method.

The object of the present invention is achieved like this:

The production method of cotton disease resistance kernel sterility dual serial GA10 of the present invention is to use anti-A ₁Anti-A is used in No. 12 hybridization of sterile strain and middle cotton institute then ₁Sterile strain is backcrossed as female parent, the sterile strain that the back of backcrossing produces again with high quality cotton strain B ₁₇Hybridization, the gained offspring hands over the strain brother and sister after continuous secondary mixes brother and sister's friendship sterile involving in a criminal case again, and forms through screening of artificial inoculation's disease resistance and fiber quality screening cultivation.

In the above-mentioned production method, kernel sterility dual serial GA10 has the recessive cytoblast sterile by sterile gene mscl control, and sterile strain ratio reaches .46-48% (theoretical ratio is 50%); The characteristics that also have the anti-verticillium wilt of the good and anti-yellows of fiber quality, fiber 2.5% is striden 31.3mm, specific strength 22.7gf/tex, micron value 4.4, fusarium wilt refers to 12.4, the verticillium wilt disease refers to 34.2.

The sterile hybrid VH of the disease-resistant nuclear of bt-cotton of the present invention ₃Production method be to utilize the sterile strain among the cotton disease resistance kernel sterility dual serial GA10 to be female parent, Insect Resistant Cotton strain HB is a male parent, adopts nuclear sterile " one is two usages ", by the hybridization of workman's supplementary pollination, produces the pest-resistant disease-resistant hybrid VH for the large tracts of land use ₃F ₁For seed.

The sterile hybrid VH of the disease-resistant nuclear of above-mentioned bt-cotton ₃Production method in the fiber quality of Insect Resistant Cotton strain HB male parent measure: 2.5% span length 30.35mm, specific strength 21.52gf/tex, Mike solemnly are worth 4.5; Good flower and two flower seed evil rates are respectively 0.8% and 4.0%, with the crossbreed VH of its production ₃Characteristics with production of hybrid seeds program simplification, disease-resistant pest-resistant, high grade producing.

The sterile hybrid VH of the disease-resistant nuclear of above-mentioned bt-cotton ₃Production method in, the propagation seed making technology main points are:

(1), mutual isolation distance is at least 100 meters between breeding field, seed farm and the Cotton Production growing area;

When (2), breeding kernel sterility dual serial GA10, make father and mother's kernel sterility dual serial originally and all need 4000～4500 strain/mus, pull out can educate strain after, guarantee that sterile strain is at 1900～2100 strain/mus, this effective strain ratio of counting of father and mother is 1: 10～12, the kernel sterility dual serial GA10 that uses as male parent requires to arrange to be planted in and plants 1 fritter on the sunny side on the ground separately behind rand or the room anterior chamber, pulls out sterile strain and stay and can educate strain and make male parent when blooming.

(3), when utilizing the kernel sterility dual serial GA10 production of hybrid seeds, make kernel sterility dual serial plantation 4000～4500 strain/mus of maternal usefulness, pull out can educate strain after, guarantee sterile strain 1900～2100 strain/mus, male parent plantation 2300～2500 strain/mus, father and mother this effectively strain to count ratio be 1: 10～12, the HB that makes male parent require before rand or room behind the house plant 1 fritter on the sunny side on the ground adopt powder and prevent to mix so that concentrate.

(4), mulch film protection cultivation, promote cotton seedling early to send out, to improve crossbreeding output.

(5), identification fertility, pull out the educated strain among the maternal kernel sterility dual serial GA10; , can educate strain and pull out to flower bud phase and florescence in growth rate of cotton plant.

(6), pollination hybridization in time, improve the genetic purity of crossbreeding output and seed: in time win the hybridization of pollinating on the column cap of the sterile strain of female parent of male parent flower or pollen after father and mother are originally bloomed, morning every day, 8 points-2 pm was a pollination time.

VH ₃The female parent of hybrid is examined sterile GA10 and is derived from ([anti-A ₁Sterile strain * (anti-A ₁No. 12, sterile strain * middle cotton institute)] F * B17) ₂Generation is sterile involves in a criminal case and continuous mix brother and sister for 2 times and hand over the strain brother and sister are handed over, and forms through screening of artificial inoculation's disease resistance and fiber quality screening cultivation.Fabric instruction of fine quality is good, 2.5 span length 29.9-31.3mm, specific strength 22.4-22.7gf/tex, micron value 4.1-46.The anti-verticillium wilt of anti-blight, the fusarium wilt disease refers to 12.4, the verticillium wilt disease refers to 34.2, lint percentage 42.1-44.5%.The characteristics of the dual-purpose GA10 of being of caryon sterile line are high-qualitys, disease-resistant.

It is Insect Resistant Cotton new lines HB that the male parent of this hybrid is recovered.Recovery is HB plant height 73.4cm, and plant is sturdy, strain shape compactness, and the medium blade size, boll per plant is strong, and single bell unginned cotton weighs 4.96 grams, lint percentage 37.3-38.3%; Fiber quality is measured, 2.5% span length 31.35mm, specific strength 21.5gf/tex, micron value 4.5; Resistance to insects good (bollworm resisting and pink bollworm); Insect pest strain on the 20th in June rate 1.6%, July 25, the fruit abscission rate rate was 3.6%, the seed evil rate of good flower and two flowers is respectively 0.8% and 4.0%.Recovery is that the characteristics of HB are pest-resistant (bollworm resisting and earworms), and high-quality, lint percentage is on the low side.

Adopt the inventive method to do a series of tests:

Winter in 1997 was done maternal with kernel sterility dual serial GA10 in Hainan, pest-resistant recovery is that HB makes male parent and surveys combo and close, and produces VH ₃(the F of GA10 * HB) ₁Seed, positive season in 1998 was carried out new combined sorting evaluation test in Sichuan, rose up into senior variety comparative test in 1999, and did not control worm and with the conventional Da Qu that controls under the worm condition analogy is tested.

In combined sorting evaluation test in 1998, do not control under the condition of worm VH in the time of infertility ₃Real 64.8 kilograms of the gined cottons of receiving, comparison are cultivated the fusarium wilt of identifying as the artificial inoculation in sick garden the plant protection chamber through this institute and are referred to be 8.0 according to river cotton 56 (Sichuan Province promotes mainly kind) volume increase 39.6%, and the verticillium wilt disease refers to be 30.0, is listed in anti-withered anti-yellow type.Fiber quality through this institute examination of fibers chamber with 900 serial HV ₁Fine inspection instrument is measured 2.5% span length 31.6mm, specific strength 22.0gf/tex, micron value 4.0.

In the senior variety comparative tests in 1999, do not control under the condition of cotton bollworm, pink bollworm VH in the time of infertility equally ₃Real receive 96.71 kilograms of gined cotton per mu yields, comparison according to the river cotton 56 every mu increase income 29.32 kilograms of gined cottons, rate of growth 43.5% reaches extremely significantly volume increase level.Cultivate the fusarium wilt disease of identifying as the artificial inoculation in sick garden the plant protection chamber through this institute and refer to be 6.8, the verticillium wilt disease refers to be 34.0, still classifies anti-withered anti-yellow type as.The gined cotton sample send Ministry of Agriculture fiber test verification center (middle cotton institute) to measure fiber 2.5% span length 29.9mm, specific strength 22.2CN/tex (being 22.7gf/tex), micron value 4.2.Insect pest strain on the 20th in June rate 2.0%, July 25, flower bud children bell expulsion rate was 4.5%, comparison is 4.5% according to river the cotton 56 94.8% and 83.0% flower bud children bell expulsion rate that goes down respectively, comparison is gone down 94.8% and 83.00% according to river cotton 56 (cotton 56 insect pest strain rates in river and two flower seeds evil rates is respectively 0.87% and 3.7% respectively, go down 96.6% and 88.2% (river cotton 56 good flowers and the harmful rates of two flower seeds are respectively 25.5% and 31.35%) according to river cotton 56 respectively, reach high anti-level.In wide field trial, just do not control cotton bollworm (pink bollworm) respectively and control worm (controlling altogether the time of infertility 11 times) with routine and made comparative trial, the result is: do not controlling under the worm condition VH ₃100.7 kilograms of real receipts gined cotton per mu yields, comparison is according to 78.25 kilograms of volume increase 28.77% of the cotton 56 per mu yield gined cottons in river; Normally controlling under the worm condition, 129.67 kilograms of real receipts gined cotton per mu yields, comparison is according to 99.72 kilograms of volume increase 30.03% of cotton 56 per mu yields in river.The result shows, is not controlling worm and is controlling under the worm condition VH ₃All extremely significantly volume increase significantly.

The present invention utilizes " hole A " type recessive nuclear gene (mscl) to derive kernel sterility dual serial GA10 for maternal, and Insect Resistant Cotton new lines HB is a male parent, and employing cotton nuclear sterile " one is two usages " breeding parent and hybridization F1 are for producing with planting.VH ₃Have following characteristics:

1, resistance to insects: this hybrid high resistance helicoverpa armigera (pink bollworm), aspect control cotton bollworm and pink bollworm, can reduce medication 70-80%.The investigation of insect pest strain on the 20th in June rate is 2.0%, July 25, flower bud children bell expulsion rate was an institute 4.5%, comparison goes down 94.8% and 83.0% according to river cotton 56 respectively, the seed evil rate of good flower and two flowers is respectively 0.87% and 3.7%, and comparison was gone down for 96.6% and 88.2% (contrast river cotton 56 good flowers and two flower seed evil rates are divided into 25.5% and 31.3%) according to river cotton 56 respectively.

2, disease resistance: the fusarium wilt disease that connects the dientification of bacteria in sick garden refers to be 6.8-8.0, and the verticillium wilt disease refers to be 30.0-34.0.Be listed in anti-withered anti-yellow type.

3, yielding ability: in 1998 and variety evaluation in 1999 test, do not control in the time of infertility under the condition of cotton bollworm and pink bollworm, the real lint yield of receiving is respectively 64.8 kilograms of per mu yields and 96.71 kilograms, and comparison is according to the cotton 56 volume increase gined cottons 39.6% and 43.5% in river respectively, increases production to reach utmost point significance level.In the wide field trials in 1999, normally controlling under the worm condition, 129.67 kilograms of real receipts gined cotton per mu yields, comparison is according to 99.72 kilograms of volume increase 30.03% of the cotton 56 per mu yield gined cottons in river; Do not controlling under worm (cotton bollworm and the pink bollworm) condition, real 100.7 kilograms of the gined cottons of receiving, comparison is according to 78.25 kilograms of volume increase 28.77% of the cotton 56 per mu yield gined cottons in river.The result shows, is controlling worm and is not controlling under the worm condition, and VH3 all shows significantly extremely significantly volume increase.

4, economic characters: fiber is neat, and color and luster is good, lint percentage 39.6-41.0%, and average single bell seed rate is cotton to be weighed about 5.7 grams.Fiber quality, 2.5% span length 29.9-31.6mm, specific strength 22.0-22.7gf/tex, micron value 4.0-4.2.Receive the flower amount before September 20 and account for total receipts flower amount 50%.

5, tree characteristics: plant height 90.6-95.0cm, plant type is tower, the plant compactness, the stem branch is sturdy, dark green leaf color, the medium blade size, light transmission is good, knot bell extra-heavy, cotton boll ovum garden is point slightly, and blow-of-cottons are smooth.

6, the time of infertility: from emerging by blow-of-cottons 137-140 days.

VH ₃Have following advantage:

1, VH ₃Collect maternal kernel sterility dual serial GA10 high-quality, disease-resistant (but not pest-resistant), male parent HB is pest-resistant, the overall merit of high-quality (but output is not high), F ₁In generation, is the output height not only, and amount of increase in production is big, and fiber quality is good, and pest-resistant disease resistance is good, and adaptability is strong.

2, VH ₃System adopts the pest-resistant F of kernel sterility dual serial preparation ₁For hybrid, compare with the hybrid of artificial emasculation preparation, production of hybrid seeds program simplification, the hybrid seed production cost reduces significantly, pest-resistant hybrid VH ₃In quality, the uniformity of resistance to insects aspect is much better than the F of artificial emasculation preparation between the individuality ₂For hybrid, genetic purity is good, and advantage is more obvious, helps standardization, the industrialization production of pest-resistant hybrid seed.

3, VH ₃Cotton bollworm, pink bollworm there are outstanding resistance, can make control worm number of times and the pesticide dosage of Cotton Production reduce 70-80%.This is to reducing the Cotton Production cost, the practice significance that increases the income of cotton grower and cotton industry and reduce effectively or avoid the pollution in cotton field to have weight very to want.

VH ₃Be applicable to the cotton regions such as Hubei, Hunan, Anhui and Jiangsu in cotton region, Sichuan and middle reaches, the Changjiang river.

Description of drawings:

Fig. 1 is GA10, VH ₃The seed selection programme diagram.

Fig. 2 is pest-resistant hybrid VH ₃Adopt nuclear sterile " one is two usages " to carry out the programme diagram that seed is produced.

Embodiment:

Referring to Fig. 1, the maternal kernel sterility dual serial GA10 that the bt-cotton hybrid produces derives from and uses earlier anti-A ₁No. 12 hybridization of sterile strain and middle cotton institute, and then use anti-A ₁Sterile strain is backcrossed as female parent, the sterile strain that backcross progeny produces again with high-quality strain B ₁₇Hybridization, F ₂, F ₃Double mixed brother and sister hand over F ₄Roguing is handed over the strain brother and sister, F ₅Plant the plant of down the strain brother and sister being handed over respectively, carry out anti-withered, the verticillium wilt evaluation of artificial inoculation seedling stage, do in the plant then to mix and brother and sister's friendship, indoor species test, fiber quality test: 2.5% strides 31.3mm, specific strength 22.7Cgf/tex, Mike swells 4.4, and fusarium wilt refers to 12.4, and the verticillium wilt disease refers to 34.2.Promptly select good brother and sister to hand over strain be kernel sterility dual serial GA10, be female parent with kernel sterility dual serial GA10 at last, with Insect Resistant Cotton strain HB serves as to recover system's preparation pest-resistant hybrid combination " VH3 ", the fiber quality of Insect Resistant Cotton strain HB male parent is measured 2.5% span length 31.35mm, specific strength 21.52gf/tex, micron value 4.5; Good flower and two flower seed evil rates are respectively 0.8% and 4.0%.

Fig. 2 is pest-resistant hybrid VH ₃It is maternal adopting caryon sterile line GA10, and pest-resistant recovery is that HB is the seed flow sheet of male parent.The left part of Fig. 2 is that isolated area 1 is the continuous reproductive process of caryon sterile line GA10, annual educate the sterile strain pollination of strain (50%) in to it being with kernel sterility dual serial GA10 is isolated, the seed of being gathered in the crops in the sterile strain is the kernel sterility dual serial GA10 that next year uses.The middle part of Fig. 2 is that isolated area II is hybrid VH ₃Production of hybrid seeds process, promptly annually kernel sterility dual serial GA10 isolated 50% can be educated strain all pull out in the flower bud phase with when blooming, make male parent to the sterile strain that keeps in the maternal district of the GA10 hybridization of pollinating with Insect Resistant Cotton strain HB, the seed of being gathered in the crops in its sterile strain is the hybrid VH of confession large tracts of land production usefulness ₃Hybrid F ₁In generation, plant.The right part of Fig. 2 is that isolated area III is pest-resistant hybrid VH ₃The land for growing field crops production process.Annual crossbreed of using all is the first generation of hybrid (F of last one year of preparation ₁) seed.Between breeding isolated area I and the seed farm isolated area II, and and the land for growing field crops produce between the growing area, isolation distance must not be less than 100 meters.

Pest-resistant hybrid VH ₃The culture technique main points:

1, suitable zone: this hybrid suits in the cotton region, Sichuan and other cotton region, Yangtze river basin demonstration plant and popularization.

2, date of seeding and sowing quantity: this hybrid be in ripe pest-resistant hybrid variety, in order to give full play to its yield potential, on sowing time, require suitably to do sth. in advance than other cotton seed, the cotton region, Sichuan of the long trip in the Changjiang river is best nursery stage at the beginning of with 4 months so far on the 20th March.Mu sowing quantity 0.5-0.75 kilogram.

3, planting density: this hybrid individual growth and knot bell advantage are stronger, and it is rare to require planting density suitably to put, and are advisable with 2200-2500 strain/mu in cotton region, Sichuan and cotton region, middle reaches, the Changjiang river.

4, fertilising: many organic fertilizers, notice that N, P, K cooperate.Suggestion mu is executed pure N amount 18-20 kilogram, and base fertilizer accounts for 15%, and fertilizers for potted flowers accounts for 45%, protects peach fertilizer and accounts for 40%; Mu is executed P ₂O ₅Amount 12-18 kilogram, base fertilizer accounts for 60%, and fertilizers for potted flowers accounts for 40%; Mu is executed K ₂O amount 12-17 kilogram, base fertilizer accounts for 70%, and fertilizers for potted flowers accounts for 30%.

5, chemical regulation: flower bud phase mu restrains with casing coupling peace 1, and visual weather and growing way spray at twice, 10-15 days at interval, converts 30 kilograms in water at every turn; Flowering stage, mu restrained with casing coupling 2.0-2.5, sprayed at twice, 15 days at interval, converted water 35-40 kilogram at every turn.Spray 920 after pinching once, every mu of medication 1 gram is converted water 15-30 kilogram, to prolong functional period of leaf.

6, pest control: note preventing and treating two spotted spider mite and cotten aphid in the seedling flower bud phase, in the control of cotton bollworm and pink bollworm, can reduce medication 70-80%, suggestion cotton bollworm (pink bollworm) take place the heavy especially time with pyrethrin pesticide time etc. the late July-early August control 1-2 time.

Pest-resistant hybrid VH ₃The propagation seed making technology main points:

1, select secure isolation zone: require to have certain distance to isolate between breeding field, seed farm and the Cotton Production growing area, isolation distance must not be less than 100 meters each other.

2, planting density and this ratio of father and mother: when breeding kernel sterility dual serial GA10, make father and mother kernel sterility dual serial GA10 originally and all need every mu of plantation 4000-4500 strain/mu, pull out can educate strain after, guarantee that sterile strain is about 2000 strain/mus, the kernel sterility dual serial GA10 that uses as male parent requires to be arranged in and plants a fritter on the sunny side on the ground separately behind rand or the room anterior chamber, pulls out sterile strain and make male parent when blooming.Ratio 1: 10-12 is counted in this effective strain of father and mother.

When utilizing the kernel sterility dual serial GA10 production of hybrid seeds, the kernel sterility dual serial GA10 that makes maternal usefulness requires to plant 4000-4500 strain/mu equally, dialling except that after can be strain, guarantees that every mu of sterile strain is about 2000 strains, male parent HB should fit to be put rarely, plants the 2300-2500 strain with mu and is advisable.Male parent HB require equally before rand or room behind the house plant a fritter on the sunny side on the ground adopt powder and prevent to mix so that concentrate.It is 1: 10～12 that ratio is counted in this effective strain of father and mother.

3, mulch film protection cultivation promotes cotton seedling early to send out, to improve crossbreeding output.

4, identification fertility property is pulled out the educated strain among the maternal kernel sterility dual serial GA10.Grow flower bud during the phase in growth rate of cotton plant, adopt flower bud phase identification fertility, promptly grow into 9-10mm when the plant bud, twist with the fingers broken flower pesticide, can educate strain and sterile strain, can educate strain and dial and remove, sterile strain is marked according to having or not pollen grain to judge with hand.Experienced also can be when bud grows into 9-10mm, judge with the circularity of hand thumb and forefinger sensation bud and can educate and sterile strain, pull out and can educate strain, sterile strain is marked, all plant are identified and finish up to full field.In order to guarantee the accuracy of flower bud phase fertility identification, also should be in the back of blooming by strain check fertility.

5, pollination hybridization in time, the genetic purity of raising crossbreeding output and seed.After father and mother are originally bloomed, in time win male parent flower (or pollen) hybridization of on the column cap of the sterile strain of female parent, pollinating.Morning every day, 8:00-2:00 in afternoon was the suitable time of pollination, and it is higher to pollinate into the bell rate around here, and seed development is good, and high efficiency, and labour intensity is low.Experiment and demonstration shows for many years, and the dwell time of the hybridization pollination of Cotton in Sichuan crossbreeding is to be advisable August 20.In the cotton region production of hybrid seeds of middle reaches, the Changjiang river, the dwell time of pollination hybridization is not later than September 5.After pollination stops, also still unopened bud in the sterile strain of female parent and empty branch all should being destroyed, cotton boll is grown so that the nutrient centralizedly supply is given pollination, and pulls out the whole plant that make male parent, thereby guarantees that a family is a kind of, guarantees the genetic purity of seed.

6, the pure and breeding of parent's's (kernel sterility dual serial GA10 and recovery are HB) guarantor.Kernel sterility dual serial GA10 and recovery are that HB should breed, protect pure in isolated area respectively, simultaneously, kernel sterility dual serial GA10 is maternal to adopt pairing individual plant brother and sister to hand over, recovery is a male parent HB individual plant selfing, screening has the brother and sister's presentate strain offspring or the selfing individual plant offspring of female parent or male parent typical case proterties, again according to the program of plant, strain system, 3 years three-field systems of original seed protect pure, expand numerous.

7, other field management is carried out routinely.

Claims (3)

1, the production method of the sterile hybrid of the disease-resistant nuclear of bt-cotton, it is characterized in that this method is to select for use the sterile strain among the cotton disease resistance kernel sterility dual serial GA10 to be female parent, Insect Resistant Cotton strain HB is a male parent, adopt nuclear sterile " one is two usages ", by artificial supplementary pollination hybridization, produce the pest-resistant disease-resistant hybrid F that uses for large tracts of land ₁For seed.

2, the production method of the sterile hybrid of the disease-resistant nuclear of bt-cotton according to claim 1 is characterized in that the fiber quality of Insect Resistant Cotton strain HB male parent is measured: 2.5% span length 31.35mm, specific strength 21.52gf/tex, micron value 4.5; Good flower and two flower seed evil rates are respectively 0.8% and 4.0%.

3, the production method of the sterile hybrid of the disease-resistant nuclear of bt-cotton according to claim 1 is characterized in that the main propagation technique that adopts is:

(1), mutual isolation distance is at least 100 meters between breeding field, seed farm and the Cotton Production growing area;

(2), during breeding kernel sterility dual serial GA10, make this kernel sterility dual serial of father and mother and all need 4000～4500 strain/mus, pull out can educate strain after, guarantee sterile strain at 1900～2100 strain/mus, the originally effective strain ratio of counting of father and mother is 1: 10～12;

(3), when utilizing the kernel sterility dual serial GA10 production of hybrid seeds, make kernel sterility dual serial plantation 4000～4500 strain/mus of maternal usefulness, pull out can educate strain after, guarantee sterile strain 1900～2100 strain/mus, male parent plantation 2300～2500 strain/mus, it is 1: 10～12 that ratio is counted in this effective strain of father and mother;

(4), mulch film protection cultivation, promote cotton seedling early to send out, to improve crossbreeding output;

(5), identification fertility, pull out the educated strain among the maternal kernel sterility dual serial GA10; , can educate strain and pull out to flower bud phase and florescence in growth rate of cotton plant;

(6), pollination hybridization in time, improve the genetic purity of crossbreeding output and seed: in time win the hybridization of pollinating on the column cap of the sterile strain of female parent of male parent flower or pollen after father and mother are originally bloomed, morning every day, 8 points-2 pm was a pollination time.

Images