CN1264401C - Hybrid seed producing method for cotton sterile line - Google Patents
Hybrid seed producing method for cotton sterile line Download PDFInfo
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- CN1264401C CN1264401C CN 200310113447 CN200310113447A CN1264401C CN 1264401 C CN1264401 C CN 1264401C CN 200310113447 CN200310113447 CN 200310113447 CN 200310113447 A CN200310113447 A CN 200310113447A CN 1264401 C CN1264401 C CN 1264401C
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- sterile line
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Abstract
The present invention relates to a hybridization seed production method of the sterile lines of cotton, which is characterized in that the seeds of the sterile lines are obtained by a hybridization method in which the pollination is completed by the assistance of insects, and basic materials comprise a recessive low phenol insect-resistant cotton strain Zhongwu R228 as a male parent and a transgenic insect-resistant genic double recessive sterile line Zhongkang A as a female parent; a transgenic insect-resistant genic double recessive sterile line with low phenol marker characters is cultivated, and the seed breeding assisted by the pollination of insects is carried out; not only is the seed production process simplified, but also the workloads of the seed breeding are reduced; simultaneously, false hybrids are easily identified, thereby avoiding economic losses. The method can be used for the large-scale production of hybridization seeds.
Description
(1) technical field
The present invention relates to a producing method for seed that grows cotton male sterile line, is a kind of producing method for seed of the bt-cotton nuclear double recessive male sterile line with low phenol mark property specifically, belongs to the plant breeding technique field.
(2) background technology
Cotton is most important in the world economic crops, and crops such as same corn, paddy rice are the same, and cotton also exists tangible hybrid vigour.The cotton crossbreed position on Cotton Production in recent years is more and more important, has become to improve the cotton per unit area yield one of main path of improvement cotton fibre quality.The mode of production of cotton hybrid seed mainly is artificial emasculation pollination's cross breeding method at present, and the hybrid seed of planting on the Cotton Production is all produced more than 90% in this way.Facts have proved that this method has time-consuming taking a lot of work, working strength is big, shortcomings such as seed farm complex management, seed production cost height.
By The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute's years of researches, successfully developing at present can be than artificial emasculation pollination's cross breeding method, and this method reduces breeding cost and labour intensity greatly, i.e. the auxiliary pollination of insect male sterile line cross breeding method.This method utilizes the transgenic pest-resistant male sterile line to do female parent, without artificial emasculation, finish pollination by insect, thereby overcome the cotton hybrid seed mode of production of present extensive use--time-consuming the taking a lot of work that-artificial emasculation pollination cross breeding method exists, working strength is big, shortcomings such as seed farm complex management, seed production cost height, and labourer's consumption can be than reducing more than 50%, and can further improve the purity of hybrid seed, be to produce the ideal approach of cotton hybrid seed.
The basis of the auxiliary pollination of insect male sterile line cross breeding method success is a transgenic pest-resistant nuclear double recessive male sterile line--in the breeding of anti-A, this material is one of cotton breeding material related to the present invention.
Anti-A is the method that The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute educates by backcrossing in the transgenic pest-resistant nuclear double recessive male sterile line, pest-resistant Bt gene transfer is bred in cotton double recessive genic male sterile line, and this achievement has been passed through colleague's expert appraisal No. 125, word (98 agricultural sections really reflect) of Ministry of Agriculture's tissue in August, 1998.The main purpose of anti-A in the seed selection is exactly when hybrid seeding, utilizes its resistance to insects and sterility, is equipped with pest-resistant male parent, realizes the auxiliary pollination simplification of the insect production of hybrid seeds.Because with in anti-A when being the maternal production of hybrid seeds, do not need artificial emasculation; Father, female parent are all pest-resistant, so between seed farm without the chemical pesticide control cotton bollworm, created nontoxic, safe, as not spray chemical pesticide environment, cotton has nectary, it is again entomophilous flower, so can replace artificial pollination by manually putting the various insects free movable insect pollination that realizes between seed farm that exists naturally in insect and the cotton field in a suitable place to breed, reach the purpose of producing hybrid seed.
In the principal character characteristic of anti-A: the plant turriform, blade is moderate, the knot bell is strong, the bell shell is thin, blow-of-cottons are smooth; The double recessive genic male sterile line, sterile plant rate about 50%, sterility is stable, thorough, sterile degree 100%; The body of gland The Characters is conventional cotton (polyphenol), can see fully aware ofly that with the naked eye there is the black body of gland of pinprick shape at positions such as its plants stems stalk, branches and leaves, bract base portion.
In the effect used of anti-A, through a large amount of evidences, the resistance to insects of this material and sterility can satisfy the requirement that the production of hybrid seeds is simplified in the auxiliary pollination of insect fully, promptly during the production of hybrid seeds without spraying pesticide, without artificial emasculation, realize insect pollination.
But because in anti-A be genic male sterile line, so educated strain about 50% can be isolated in its plantation field, be mixed in randomly, at random between the sterile strain, must before the production of hybrid seeds begins, thoroughly pull out, otherwise will produce pseudostationary, reduce seed production purity.Can to educate strain be very difficult and will pull out these fully, up hill and dale, then almost is impossible during the extensive production of hybrid seeds.Because in order to guarantee the output of the production of hybrid seeds, must guarantee the sterile strain of certain density, so in the thickness of sowing of anti-A seed farm be 2 times of conventional seed farm, and can educate strain and only budding and young flower bud could be differentiated out when growing to a certain degree in cotton, so having caused the fertility of all maternal plants can differentiate before the production of hybrid seeds begins, seed farm unbalanced be difficult to realize; Even can differentiate, because plant is dense, artificial careless omission also is difficult to avoid.Can educate strain can't be fully, pull out up hill and dale, its pollen will be given male sterile line by insect pollination, also can natural Cheng Ling, caused certainly leading to and can't differentiating of pseudostationary, after next year plants these crossbreed that contain pseudostationary, can isolate a large amount of sterile strains, cause unnecessary economic loss.
The present invention utilizes the auxiliary pollination of insect male sterile line cross breeding method, with anti-A in the transgenic pest-resistant nuclear double recessive male sterile line is basic material, cultivate the transgenic pest-resistant nuclear double recessive male sterile line that has low phenol mark property and carry out the auxiliary pollination of insect breeding, not only simplify production of hybrid seeds program, reduced the breeding work amount, simultaneously on the seedbed or cutting kind of benevolence range estimation having or not of pigment gland can distinguish true and false hybrid, what pigment gland was arranged then is true crossbreed, otherwise be false crossbreed, thereby avoided economic loss, can be used for the large-scale production hybrid seed.
(3) summary of the invention
[goal of the invention]
The objective of the invention is to cultivate the transgenic pest-resistant nuclear double recessive male sterile line that has recessive low phenol mark property and carry out the auxiliary pollination of the insect production of hybrid seeds, take a lot of work to solve the time-consuming of present cotton hybrid seeding technique, working strength is big, and causes a series of problems such as pseudostationary is many.
[technical scheme]
Technical scheme of the present invention is through sexual hybridization, during recessive non-gland gene is transferred among the anti-A, in its offspring's fertility segregation population, do strain to strain hybridization (sterile * as can to educate), divide the contracture kind, investigate having or not of its offspring's fertility separating ratio and body of gland, and observe in conjunction with economical character, select through multiple bearing for many years, that finishing screen is selected is pest-resistant, (low phenol standard: the gossypol content in the low-phenol cotton kind benevolence of WHO and the World Food Programme's regulation is lower than 0.04% to low phenol, and the low phenol standard of China's regulation is that gossypol content is lower than 0.02%.Low phenol content in the low phenol material kind benevolence involved in the present invention all is lower than state-set standard), sterile plant rate is stable shows as 50% sterile line material, and is specific as follows:
1, the transformation of recessive low phenol half maintenance line.Utilize in the low phenol Insect Resistant Cotton strain of known recessiveness no R228 do male parent with in anti-A hybridize F for female parent
1Take Hainan Island and add generation, from F
2Choose low phenol in generation and can educate strain and work as male parent, do the hybridization of strain, divide by strain and receive strain with the sterile strain that separates, plantation in 1 year, the investigation fertility, the reservation sterile plant rate is 50% plant.
2, low phenol double recessive genic male sterile line purifying.To sterile plant rate is that 50% plant carries out body of gland observation, and the sterile strain of choosing the educated strain of low phenol and low phenol is carried out strain strain is hybridized, and divides by strain and receives crossbreed and test cross kind.Utilize Hainan Island to add generation winter, 1 year two season, manually put honeybee pollination in a suitable place to breed, to strain hybridization and test cross, select the stable pest-resistant low phenol double recessive genic male sterile line of proterties through continuous 3 years strain, this male sterile line comprehensive agronomy proterties is good, high resistance helicoverpa armigera, plant turriform, blade are moderate, the knot bell is strong, the bell shell is thin, blow-of-cottons are smooth, sterile degree 100%, and sterility is stable, thoroughly.
[beneficial effect]
The present invention compared with prior art has following advantage:
1 compares with existing artificial emasculation pollination's cross breeding method, and the present invention can significantly reduce the labour intensity between seed farm, reduces workload, improves production of hybrid seeds work efficiency, thereby reduces breeding cost significantly.
2, with of the present invention sterile be maternal, be easy to distinguish true and false crossbreed, and on the seedbed, can pull out easily, can large tracts of land, on a large scale by the field production of hybrid seeds of the auxiliary pollination of insect male sterile line.
3, it is extensive that male sterile line of the present invention recovers the source, and general upland cotton kind (being) all can be used as it and recovers system, and hybrid vigour is obvious, and competitive advantage is 115-136%, and pest-resistant effective.
4, with conventional variety relatively, hybrid vigour situation of the present invention relatively: utilizing material of the present invention is assorted A49-668 in the crossbreed of female parent preparation, integrates high yield, pest-resistant, the simplification production of hybrid seeds, the output height, resistance to insects is good.In 2001-2002 participates in institute of middle cotton institute in each strain of participating in the experiment of strain rating test, in assorted A49-668 two annual lint yields and pre-frost lint yield be respectively 94.6 kilograms/mu and 99.8 kilograms/mu, increase production 13.9% and 15.3% respectively than check variety middle cotton institute 38, equal tool first place, volume increase reaches utmost point significance level, and bollworm resisting reaches the secondary resistance level.Disease resistance: fusarium wilt refers to be 2.5, and verticillium wilt refers to be 26.8, and response type is anti-withered anti-Huang.Fiber quality: through the test in 2002 of Ministry of Agriculture cotton quality supervision center, in the assorted average 2.5% span length 30.2mm of A49-668, specific strength 31.4cN/tex, micron value 4.8.
And because in the female parent of assorted A49-668 be the transgenic pest-resistant nuclear double recessive male sterile line that has recessive low phenol mark property, really realized the auxiliary pollination of insect male sterile line cross breeding method large-scale production hybrid seed, can significantly reduce cost of cotton plantation and improve the purity of crossbreed greatly than the recruitment of general cross cotton variety minimizing more than 60% during the production of hybrid seeds.
(4) description of drawings
Accompanying drawing 1 is a breeding line spectrogram of the present invention
In the accompanying drawing
I: in the cultivating process of anti-A
II: the cultivating process of the recessive low pest-resistant caryon sterile line of phenol
III: the guarantor of the recessive low pest-resistant caryon sterile line of phenol plants breeding
IV: the hybrid seeding of the recessive low pest-resistant caryon sterile line of phenol
★: manually put honeybee pollination in a suitable place to breed
Ms
5Ms
5Ms
6Ms
6: double recessive kernel male sterile genotype
RR: anti insect gene
Gl
2Gl
2: dominance has gland gene
Gl
2Gl
2: recessive non-gland gene
RRms
5Ms
5Ms
6Ms
6Gl
2Gl
2: the genotype of the recessive low pest-resistant caryon sterile line of phenol
RRMs
5Ms
5Ms
6Ms
6Gl
2Gl
2(RRms
5Ms
5Ms
6Ms
6Gl
2Gl
2): the genotype of recessive low pest-resistant caryon sterile line half maintenance line of phenol
(5) embodiment
1, seed selection process:
With ms
5Ms
6The sterile gene transformation has been arrived in the good kind of high yield, high-quality, comprehensive agronomy proterties, cultivating sterile plant rate is 50%, the two latent caryon sterile line of heterogeneous heteronuclear that economical character is good, be female parent with it again, with the transgenic cotton against pests product is paternal hybrid, to its offspring through backcrossing, test cross, it is good finally to have bred existing resistance to insects, sterility is stable, anti-A in the transgenic pest-resistant male sterile line completely.
Utilize in the recessive low phenol Insect Resistant Cotton strain no R228 do male parent and in anti-A hybridize F
1Take Hainan Island and add generation, from F
2Choose the educated strain of low phenol (promptly estimating the non-pigment body of gland) in generation and work as male parent, do the hybridization of strain, press strain and divide receipts strain with the sterile strain that separates, plantation in 1 year, the investigation fertility, the reservation sterile plant rate is 50% plant.
To sterile plant rate is that 50% plant carries out body of gland observation, and the sterile strain of choosing the educated strain of low phenol and low phenol is carried out strain strain is hybridized, and divides by strain and receives crossbreed and test cross kind.Utilize Hainan Island to add generation winter, 1 year two season, manually put honeybee pollination in a suitable place to breed, pass through continuous 3 years strain to strain hybridization and test cross, select the stable pest-resistant low phenol double recessive genic male sterile line of proterties, this male sterile line comprehensive agronomy proterties good (bollworm resisting, plant turriform, blade are moderate, the bell shell thin, blow-of-cottons are smooth, sterile degree 100%, sterility is stable, thorough).
2, the present invention has the major physiological fertility feature of the transgenic pest-resistant nuclear double recessive male sterile line of recessive low phenol mark property: bollworm resisting reaches the secondary resistance, plant turriform, blade are moderate, the bell shell thin, blow-of-cottons are smooth, sterile degree 100%, sterility is stable, thorough; Test cross strain ginning outturn about 40%, single bell weigh about 5.5 grams, about 20 of boll per plants.
3, the hybrid seeding of the recessive low pest-resistant caryon sterile line of phenol
With the recessive low pest-resistant caryon sterile line of phenol of the present invention is female parent, and conventional cotton variety is a paternal hybrid, manually puts honeybee pollination in a suitable place to breed, F
1For the true crossbreed that is of body of gland performance consistent with male parent (polyphenol), the body of gland performance is false crossbreed just with maternal consistent (low phenol).
Though above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, not departing from the these modifications or improvements basically of spirit of the present invention, all belong to the scope of protection of present invention.
Claims (1)
1. a cross-breeding method that grows cotton male sterile line is characterized in that:
A. make anti-A hybridization in male parent and the transgenic pest-resistant male sterile line, F with no R228 in the recessive low-phenol cotton strain
1Hainan adds generation, from F
2Choosing low phenol in generation, can to educate strain be male parent, does strain with the sterile strain that separates strain is hybridized, and divides by strain and receive, and the plantation in next year keeps sterile plant rate and be 50% plant;
B. be that 50% plant carries out body of gland observation to sterile plant rate, the sterile strain of choosing the educated strain of low phenol and low phenol is carried out strain strain is hybridized, and divides by strain and receives crossbreed and test cross kind; Utilize Hainan Island to add generation in winter, 1 year two season, manually put honeybee pollination in a suitable place to breed, to strain hybridization and test cross, select the stable pest-resistant low phenol double recessive genic male sterile line of proterties through continuous 3 years strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310113447 CN1264401C (en) | 2003-11-10 | 2003-11-10 | Hybrid seed producing method for cotton sterile line |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310113447 CN1264401C (en) | 2003-11-10 | 2003-11-10 | Hybrid seed producing method for cotton sterile line |
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| CN1615688A CN1615688A (en) | 2005-05-18 |
| CN1264401C true CN1264401C (en) | 2006-07-19 |
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| CN 200310113447 Expired - Fee Related CN1264401C (en) | 2003-11-10 | 2003-11-10 | Hybrid seed producing method for cotton sterile line |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415332B (en) * | 2011-09-29 | 2013-08-28 | 中国农业科学院棉花研究所 | Breeding method of yellow bud marker photosensitive male sterile line |
| CN103493728B (en) * | 2013-08-28 | 2016-04-06 | 山东农兴种业有限责任公司 | The color cotton sterile line breeding of a kind of pest-resistant low phenol and hybrid breeding method |
| CN103493724B (en) * | 2013-08-28 | 2016-04-27 | 山东农兴种业有限责任公司 | The cotton seed selection of a kind of pest-resistant low phenol stigma appearing double-line hybrid and cross-breeding method |
| CN113207681B (en) * | 2021-05-31 | 2022-10-28 | 河北省农林科学院棉花研究所（河北省农林科学院特种经济作物研究所） | Breeding method of recessive glandless cotton |
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