CN102858952B - 改进的细胞培养基 - Google Patents

改进的细胞培养基 Download PDF

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Publication number
CN102858952B
CN102858952B CN201180021024.XA CN201180021024A CN102858952B CN 102858952 B CN102858952 B CN 102858952B CN 201180021024 A CN201180021024 A CN 201180021024A CN 102858952 B CN102858952 B CN 102858952B
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cell
culture medium
cell culture
substratum
ion
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Chinese (zh)
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CN102858952A (zh
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C·莱斯特
P·迈斯纳
J·施密特
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Novartis AG
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Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
CN201180021024.XA 2010-04-26 2011-04-25 改进的细胞培养基 Active CN102858952B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783610P 2010-04-26 2010-04-26
US61/327,836 2010-04-26
PCT/EP2011/056508 WO2011134920A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Publications (2)

Publication Number Publication Date
CN102858952A CN102858952A (zh) 2013-01-02
CN102858952B true CN102858952B (zh) 2015-10-14

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Family Applications (1)

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CN201180021024.XA Active CN102858952B (zh) 2010-04-26 2011-04-25 改进的细胞培养基

Country Status (17)

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US (2) USRE48864E1 (e)
EP (1) EP2563904B1 (e)
JP (3) JP6347949B2 (e)
KR (1) KR101828623B1 (e)
CN (1) CN102858952B (e)
AU (1) AU2011246503B2 (e)
BR (1) BR112012027282B1 (e)
CA (1) CA2797356C (e)
DK (1) DK2563904T3 (e)
ES (1) ES2533074T3 (e)
IL (1) IL222452B (e)
MX (1) MX2012012526A (e)
PL (1) PL2563904T3 (e)
PT (1) PT2563904E (e)
RU (1) RU2644651C2 (e)
SG (1) SG185038A1 (e)
WO (1) WO2011134920A1 (e)

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US10493151B2 (en) 2011-10-18 2019-12-03 Coherus Biosciences, Inc. Etanercept formulations stabilized with sodium chloride
WO2013059406A1 (en) 2011-10-18 2013-04-25 Coherus Biosciences, Inc. Etanercept formulations stabilized with metal ions
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US20130281355A1 (en) 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
EP2869817A4 (en) 2012-07-09 2016-04-06 Coherus Biosciences Inc STABLE AQUEOUS FORMULATIONS OF ETANERCEPT
KR20150043523A (ko) 2012-09-02 2015-04-22 애브비 인코포레이티드 단백질 불균일성의 제어 방법
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
BR112015005161A2 (pt) 2012-09-11 2017-07-04 Coherus Biosciences Inc etanercepte corretamente dobrado em alta pureza e excelente rendimento
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014158231A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. Low acidic species compositions and methods for producing and using the same
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
BR112016002622B1 (pt) 2013-08-20 2023-04-11 Lek Pharmaceuticals D.D Meio de cultura celular e uso de selênio
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
KR102510238B1 (ko) * 2013-10-11 2023-03-16 리제너론 파마슈티칼스 인코포레이티드 대사적으로 최적화된 세포 배양
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
JP6749838B2 (ja) * 2014-01-30 2020-09-02 コヒラス・バイオサイエンシズ・インコーポレイテッド かん流培地
LT3137595T (lt) 2014-04-29 2019-06-25 Novartis Ag Naujos stuburinių ląstelės ir dominančio polipeptido rekombinantinės raiškos būdai
EP2975050B1 (en) 2014-07-18 2019-05-29 Sandoz Ag Quantification of misfolded TNFR2:Fc
WO2017106783A1 (en) * 2015-12-17 2017-06-22 Life Technologies Corporation Pellets used in cell culture and methods of making thereof
IL261999B2 (en) * 2016-04-05 2023-04-01 Pfizer Cell culture process
US11702628B2 (en) 2017-03-31 2023-07-18 Boehringer Ingelheim International Gmbh Perfusion medium for culturing mammalian cells and reducing cell bleed in a perfusion cell culture
WO2018178063A1 (en) * 2017-03-31 2018-10-04 Boehringer Ingelheim International Gmbh Perfusion medium
JPWO2021090888A1 (e) * 2019-11-05 2021-05-14
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UY39484A (es) 2020-11-02 2022-06-30 Novartis Ag Inhibidores de interleucina-17

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Also Published As

Publication number Publication date
US9428727B2 (en) 2016-08-30
AU2011246503B2 (en) 2013-09-26
JP7034991B2 (ja) 2022-03-14
ES2533074T3 (es) 2015-04-07
RU2018101201A3 (e) 2021-05-27
USRE48864E1 (en) 2021-12-28
RU2012150346A (ru) 2014-06-10
EP2563904B1 (en) 2015-01-21
IL222452B (en) 2019-03-31
PT2563904E (pt) 2015-05-12
WO2011134920A1 (en) 2011-11-03
IL222452A0 (en) 2012-12-31
CA2797356C (en) 2020-12-29
US20130122543A1 (en) 2013-05-16
RU2644651C2 (ru) 2018-02-13
CN102858952A (zh) 2013-01-02
AU2011246503A1 (en) 2012-11-22
JP2019216728A (ja) 2019-12-26
CA2797356A1 (en) 2011-11-03
BR112012027282B1 (pt) 2020-09-29
JP2013524825A (ja) 2013-06-20
EP2563904A1 (en) 2013-03-06
KR20130058699A (ko) 2013-06-04
MX2012012526A (es) 2012-11-23
SG185038A1 (en) 2012-11-29
JP6603628B2 (ja) 2019-11-06
JP6347949B2 (ja) 2018-06-27
BR112012027282A2 (pt) 2015-09-22
DK2563904T3 (en) 2015-04-07
KR101828623B1 (ko) 2018-02-12
RU2018101201A (ru) 2019-02-21
JP2017012180A (ja) 2017-01-19
PL2563904T3 (pl) 2015-06-30

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