IL222452B - A process for preparing a recombinant polypeptide by growing mammalian cells in a serum-free and protein-free cell culture medium - Google Patents
A process for preparing a recombinant polypeptide by growing mammalian cells in a serum-free and protein-free cell culture mediumInfo
- Publication number
- IL222452B IL222452B IL222452A IL22245212A IL222452B IL 222452 B IL222452 B IL 222452B IL 222452 A IL222452 A IL 222452A IL 22245212 A IL22245212 A IL 22245212A IL 222452 B IL222452 B IL 222452B
- Authority
- IL
- Israel
- Prior art keywords
- production
- culture medium
- cell culture
- mammalian cells
- recombinant polypeptide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0031—Serum-free culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/005—Protein-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32783610P | 2010-04-26 | 2010-04-26 | |
| PCT/EP2011/056508 WO2011134920A1 (en) | 2010-04-26 | 2011-04-25 | Improved cell culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL222452A0 IL222452A0 (en) | 2012-12-31 |
| IL222452B true IL222452B (en) | 2019-03-31 |
Family
ID=44074260
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL222452A IL222452B (en) | 2010-04-26 | 2012-10-15 | A process for preparing a recombinant polypeptide by growing mammalian cells in a serum-free and protein-free cell culture medium |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US9428727B2 (e) |
| EP (1) | EP2563904B1 (e) |
| JP (3) | JP6347949B2 (e) |
| KR (1) | KR101828623B1 (e) |
| CN (1) | CN102858952B (e) |
| AU (1) | AU2011246503B2 (e) |
| BR (1) | BR112012027282B1 (e) |
| CA (1) | CA2797356C (e) |
| DK (1) | DK2563904T3 (e) |
| ES (1) | ES2533074T3 (e) |
| IL (1) | IL222452B (e) |
| MX (1) | MX2012012526A (e) |
| PL (1) | PL2563904T3 (e) |
| PT (1) | PT2563904E (e) |
| RU (1) | RU2644651C2 (e) |
| SG (1) | SG185038A1 (e) |
| WO (1) | WO2011134920A1 (e) |
Families Citing this family (41)
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| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| NZ575328A (en) | 2006-09-13 | 2012-06-29 | Abbott Lab | Cell culture improvements |
| JP5808249B2 (ja) | 2008-10-20 | 2015-11-10 | アッヴィ・インコーポレイテッド | プロテインａアフィニティークロマトグラフィーを用いる抗体の単離および精製 |
| AU2009307737B2 (en) | 2008-10-20 | 2015-07-23 | Abbvie Inc. | Viral inactivation during purification of antibodies |
| MX2012012525A (es) | 2010-04-26 | 2012-11-23 | Novartis Ag | Medio de cultivo mejorado. |
| DK2563904T3 (en) * | 2010-04-26 | 2015-04-07 | Novartis Ag | Improved cell culture medium |
| BR112013009934A2 (pt) * | 2010-12-07 | 2016-07-05 | Hoffmann La Roche | dispositivo para adicionar pelo menos duas soluções de alimentação, aparelho de cultivo celular, uso de um dispositivo e método para obter um polipeptídeo |
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| US10493151B2 (en) | 2011-10-18 | 2019-12-03 | Coherus Biosciences, Inc. | Etanercept formulations stabilized with sodium chloride |
| AU2012326171B2 (en) | 2011-10-18 | 2017-03-09 | Coherus Biosciences, Inc. | Etanercept formulations stabilized with sodium chloride |
| US9181572B2 (en) | 2012-04-20 | 2015-11-10 | Abbvie, Inc. | Methods to modulate lysine variant distribution |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US20130281355A1 (en) | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
| US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
| EP2869816A4 (en) | 2012-07-09 | 2016-04-20 | Coherus Biosciences Inc | FORMANTS OF ETANERCEPT HAVING A REDUCTION MARKED IN INVISIBLE PARTICLES IN THE NU |
| CA2883272A1 (en) | 2012-09-02 | 2014-03-06 | Abbvie Inc. | Methods to control protein heterogeneity |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| DK2895188T3 (en) | 2012-09-11 | 2018-02-26 | Coherus Biosciences Inc | CORRECTLY FOLDED ETHNECCEPT IN HIGH PURITY AND EXCELLENT YIELD |
| CA2905010A1 (en) | 2013-03-12 | 2014-09-18 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
| EP2836515A1 (en) | 2013-03-14 | 2015-02-18 | AbbVie Inc. | Low acidic species compositions and methods for producing and using the same |
| US10196601B2 (en) | 2013-08-20 | 2019-02-05 | Lek Pharmaceuticals D.D. | Cell culture medium and process for controlling α-amidation and/or C-terminal amino acid cleavage of polypeptides |
| WO2015051293A2 (en) | 2013-10-04 | 2015-04-09 | Abbvie, Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| MX2016004564A (es) * | 2013-10-11 | 2016-07-12 | Regeneron Pharma | Cultivo celular metabolicamente optimizado. |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| WO2015116820A1 (en) | 2014-01-30 | 2015-08-06 | Coherus Biosciences, Inc. | Perfusion media |
| DK3536776T3 (da) | 2014-04-29 | 2024-01-15 | Novartis Ag | Hidtil ukendte vertebratceller og fremgangsmåder til rekombinant ekspression af et polypeptid af interesse |
| EP2975050B1 (en) | 2014-07-18 | 2019-05-29 | Sandoz Ag | Quantification of misfolded TNFR2:Fc |
| US10822584B2 (en) * | 2015-12-17 | 2020-11-03 | Life Technologies Corporation | Pellets used in cell culture and methods of making thereof |
| EP3440190A1 (en) * | 2016-04-05 | 2019-02-13 | Pfizer Inc | Cell culture process |
| CN110520538B (zh) * | 2017-03-31 | 2024-02-20 | 勃林格殷格翰国际公司 | 灌注培养基 |
| WO2018178063A1 (en) * | 2017-03-31 | 2018-10-04 | Boehringer Ingelheim International Gmbh | Perfusion medium |
| JPWO2021090888A1 (e) * | 2019-11-05 | 2021-05-14 | ||
| WO2022036275A2 (en) * | 2020-08-14 | 2022-02-17 | Bristol-Myers Squibb Company | Manufacturing process for protein |
| UY39484A (es) | 2020-11-02 | 2022-06-30 | Novartis Ag | Inhibidores de interleucina-17 |
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| US5672502A (en) | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
| EP0283942B1 (en) | 1987-03-24 | 1992-05-20 | W.R. Grace & Co.-Conn. | Basal nutrient medium for cell culture |
| US6048728A (en) * | 1988-09-23 | 2000-04-11 | Chiron Corporation | Cell culture medium for enhanced cell growth, culture longevity, and product expression |
| US5135866A (en) | 1989-03-03 | 1992-08-04 | W. R. Grace & Co.-Conn. | Very low protein nutrient medium for cell culture |
| CA2001550A1 (en) | 1989-03-03 | 1990-09-03 | Aaron H. Heifetz | Very low protein nutrient medium for cell culture |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| JPH0789908B2 (ja) | 1991-02-28 | 1995-10-04 | 倉敷紡績株式会社 | 動物細胞培養用無血清培地 |
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| US5856179A (en) | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
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| US5804420A (en) | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
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| GB0304799D0 (en) * | 2003-03-03 | 2003-04-09 | Glaxosmithkline Biolog Sa | Novel method |
| DE10338536A1 (de) | 2003-08-19 | 2005-04-07 | Bircher Reglomat Ag | Verfahren zum Betreiben eines Radarsensors |
| TWI384069B (zh) * | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
| TWI374935B (en) | 2004-08-27 | 2012-10-21 | Pfizer Ireland Pharmaceuticals | Production of α-abeta |
| EP1707634A1 (en) | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
| EP1937798A4 (en) | 2005-04-15 | 2009-05-13 | Univ Columbia | CHEMICALLY DEFINED CULTURAL MEDIA FOR THE EXPANSION AND DIFFERENTIATION OF EPIDERMIC CELLS AND USES THEREOF FOR THE IN VITRO FARMING OF HAIRFOLLICLES |
| US20070231895A1 (en) | 2005-11-02 | 2007-10-04 | Lee Gene W | Methods for adapting mammalian cells |
| US20070190057A1 (en) | 2006-01-23 | 2007-08-16 | Jian Wu | Methods for modulating mannose content of recombinant proteins |
| JP5564259B2 (ja) * | 2006-11-08 | 2014-07-30 | ワイス・エルエルシー | 細胞培養のための合理的に設計された培地 |
| DK2563904T3 (en) * | 2010-04-26 | 2015-04-07 | Novartis Ag | Improved cell culture medium |
-
2011
- 2011-04-25 DK DK11717240T patent/DK2563904T3/en active
- 2011-04-25 WO PCT/EP2011/056508 patent/WO2011134920A1/en active Application Filing
- 2011-04-25 US US13/643,998 patent/US9428727B2/en not_active Ceased
- 2011-04-25 PL PL11717240T patent/PL2563904T3/pl unknown
- 2011-04-25 AU AU2011246503A patent/AU2011246503B2/en active Active
- 2011-04-25 PT PT117172403T patent/PT2563904E/pt unknown
- 2011-04-25 EP EP11717240.3A patent/EP2563904B1/en not_active Revoked
- 2011-04-25 CA CA2797356A patent/CA2797356C/en active Active
- 2011-04-25 CN CN201180021024.XA patent/CN102858952B/zh active Active
- 2011-04-25 KR KR1020127030709A patent/KR101828623B1/ko active IP Right Grant
- 2011-04-25 JP JP2013506612A patent/JP6347949B2/ja active Active
- 2011-04-25 ES ES11717240.3T patent/ES2533074T3/es active Active
- 2011-04-25 BR BR112012027282-9A patent/BR112012027282B1/pt active IP Right Grant
- 2011-04-25 SG SG2012079174A patent/SG185038A1/en unknown
- 2011-04-25 RU RU2012150346A patent/RU2644651C2/ru active
- 2011-04-25 MX MX2012012526A patent/MX2012012526A/es active IP Right Grant
- 2011-04-25 US US16/114,731 patent/USRE48864E1/en active Active
-
2012
- 2012-10-15 IL IL222452A patent/IL222452B/en active IP Right Grant
-
2016
- 2016-08-03 JP JP2016152747A patent/JP6603628B2/ja active Active
-
2019
- 2019-08-01 JP JP2019142124A patent/JP7034991B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| BR112012027282B1 (pt) | 2020-09-29 |
| JP7034991B2 (ja) | 2022-03-14 |
| EP2563904A1 (en) | 2013-03-06 |
| CA2797356C (en) | 2020-12-29 |
| EP2563904B1 (en) | 2015-01-21 |
| CN102858952B (zh) | 2015-10-14 |
| SG185038A1 (en) | 2012-11-29 |
| KR101828623B1 (ko) | 2018-02-12 |
| RU2018101201A (ru) | 2019-02-21 |
| US20130122543A1 (en) | 2013-05-16 |
| JP2013524825A (ja) | 2013-06-20 |
| AU2011246503A1 (en) | 2012-11-22 |
| DK2563904T3 (en) | 2015-04-07 |
| WO2011134920A1 (en) | 2011-11-03 |
| JP2019216728A (ja) | 2019-12-26 |
| JP6347949B2 (ja) | 2018-06-27 |
| CA2797356A1 (en) | 2011-11-03 |
| AU2011246503B2 (en) | 2013-09-26 |
| JP6603628B2 (ja) | 2019-11-06 |
| PL2563904T3 (pl) | 2015-06-30 |
| JP2017012180A (ja) | 2017-01-19 |
| BR112012027282A2 (pt) | 2015-09-22 |
| RU2644651C2 (ru) | 2018-02-13 |
| US9428727B2 (en) | 2016-08-30 |
| USRE48864E1 (en) | 2021-12-28 |
| CN102858952A (zh) | 2013-01-02 |
| PT2563904E (pt) | 2015-05-12 |
| RU2012150346A (ru) | 2014-06-10 |
| MX2012012526A (es) | 2012-11-23 |
| IL222452A0 (en) | 2012-12-31 |
| RU2018101201A3 (e) | 2021-05-27 |
| ES2533074T3 (es) | 2015-04-07 |
| KR20130058699A (ko) | 2013-06-04 |
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Legal Events
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|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed |