RU2644651C2 - Среда для культивирования клеток - Google Patents

Среда для культивирования клеток Download PDF

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Publication number
RU2644651C2
RU2644651C2 RU2012150346A RU2012150346A RU2644651C2 RU 2644651 C2 RU2644651 C2 RU 2644651C2 RU 2012150346 A RU2012150346 A RU 2012150346A RU 2012150346 A RU2012150346 A RU 2012150346A RU 2644651 C2 RU2644651 C2 RU 2644651C2
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cells
medium
concentration
ions
cell culture
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RU2012150346A
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English (en)
Russian (ru)
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RU2012150346A (ru
Inventor
Кристиан ЛЯЙСТ
Петра МАЙССНЕР
Йорг ШМИДТ
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Новартис Аг
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Application filed by Новартис Аг filed Critical Новартис Аг
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
RU2012150346A 2010-04-26 2011-04-25 Среда для культивирования клеток RU2644651C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783610P 2010-04-26 2010-04-26
US61/327,836 2010-04-26
PCT/EP2011/056508 WO2011134920A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Related Child Applications (1)

Application Number Title Priority Date Filing Date
RU2018101201A Division RU2798069C2 (ru) 2010-04-26 2011-04-25 Улучшенная среда для культивирования клеток

Publications (2)

Publication Number Publication Date
RU2012150346A RU2012150346A (ru) 2014-06-10
RU2644651C2 true RU2644651C2 (ru) 2018-02-13

Family

ID=44074260

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2012150346A RU2644651C2 (ru) 2010-04-26 2011-04-25 Среда для культивирования клеток

Country Status (17)

Country Link
US (2) US9428727B2 (e)
EP (1) EP2563904B1 (e)
JP (3) JP6347949B2 (e)
KR (1) KR101828623B1 (e)
CN (1) CN102858952B (e)
AU (1) AU2011246503B2 (e)
BR (1) BR112012027282B1 (e)
CA (1) CA2797356C (e)
DK (1) DK2563904T3 (e)
ES (1) ES2533074T3 (e)
IL (1) IL222452B (e)
MX (1) MX2012012526A (e)
PL (1) PL2563904T3 (e)
PT (1) PT2563904E (e)
RU (1) RU2644651C2 (e)
SG (1) SG185038A1 (e)
WO (1) WO2011134920A1 (e)

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EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US10493151B2 (en) 2011-10-18 2019-12-03 Coherus Biosciences, Inc. Etanercept formulations stabilized with sodium chloride
AU2012326171B2 (en) 2011-10-18 2017-03-09 Coherus Biosciences, Inc. Etanercept formulations stabilized with sodium chloride
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US20130281355A1 (en) 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
EP2869816A4 (en) 2012-07-09 2016-04-20 Coherus Biosciences Inc FORMANTS OF ETANERCEPT HAVING A REDUCTION MARKED IN INVISIBLE PARTICLES IN THE NU
CA2883272A1 (en) 2012-09-02 2014-03-06 Abbvie Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
DK2895188T3 (en) 2012-09-11 2018-02-26 Coherus Biosciences Inc CORRECTLY FOLDED ETHNECCEPT IN HIGH PURITY AND EXCELLENT YIELD
CA2905010A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
EP2836515A1 (en) 2013-03-14 2015-02-18 AbbVie Inc. Low acidic species compositions and methods for producing and using the same
US10196601B2 (en) 2013-08-20 2019-02-05 Lek Pharmaceuticals D.D. Cell culture medium and process for controlling α-amidation and/or C-terminal amino acid cleavage of polypeptides
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
MX2016004564A (es) * 2013-10-11 2016-07-12 Regeneron Pharma Cultivo celular metabolicamente optimizado.
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
WO2015116820A1 (en) 2014-01-30 2015-08-06 Coherus Biosciences, Inc. Perfusion media
DK3536776T3 (da) 2014-04-29 2024-01-15 Novartis Ag Hidtil ukendte vertebratceller og fremgangsmåder til rekombinant ekspression af et polypeptid af interesse
EP2975050B1 (en) 2014-07-18 2019-05-29 Sandoz Ag Quantification of misfolded TNFR2:Fc
US10822584B2 (en) * 2015-12-17 2020-11-03 Life Technologies Corporation Pellets used in cell culture and methods of making thereof
EP3440190A1 (en) * 2016-04-05 2019-02-13 Pfizer Inc Cell culture process
CN110520538B (zh) * 2017-03-31 2024-02-20 勃林格殷格翰国际公司 灌注培养基
WO2018178063A1 (en) * 2017-03-31 2018-10-04 Boehringer Ingelheim International Gmbh Perfusion medium
JPWO2021090888A1 (e) * 2019-11-05 2021-05-14
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Also Published As

Publication number Publication date
BR112012027282B1 (pt) 2020-09-29
JP7034991B2 (ja) 2022-03-14
EP2563904A1 (en) 2013-03-06
CA2797356C (en) 2020-12-29
EP2563904B1 (en) 2015-01-21
CN102858952B (zh) 2015-10-14
SG185038A1 (en) 2012-11-29
KR101828623B1 (ko) 2018-02-12
RU2018101201A (ru) 2019-02-21
US20130122543A1 (en) 2013-05-16
JP2013524825A (ja) 2013-06-20
AU2011246503A1 (en) 2012-11-22
DK2563904T3 (en) 2015-04-07
WO2011134920A1 (en) 2011-11-03
JP2019216728A (ja) 2019-12-26
JP6347949B2 (ja) 2018-06-27
CA2797356A1 (en) 2011-11-03
AU2011246503B2 (en) 2013-09-26
JP6603628B2 (ja) 2019-11-06
PL2563904T3 (pl) 2015-06-30
JP2017012180A (ja) 2017-01-19
BR112012027282A2 (pt) 2015-09-22
IL222452B (en) 2019-03-31
US9428727B2 (en) 2016-08-30
USRE48864E1 (en) 2021-12-28
CN102858952A (zh) 2013-01-02
PT2563904E (pt) 2015-05-12
RU2012150346A (ru) 2014-06-10
MX2012012526A (es) 2012-11-23
IL222452A0 (en) 2012-12-31
RU2018101201A3 (e) 2021-05-27
ES2533074T3 (es) 2015-04-07
KR20130058699A (ko) 2013-06-04

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