CN102836368A - Chinese medicine composite for treating bee diseases and preparation method thereof - Google Patents
Chinese medicine composite for treating bee diseases and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a Chinese medicine composite for treating bee diseases and a preparation method of the Chinese medicine composite. The Chinese medicine composite for treating bee diseases comprises the following raw materials in parts by weight: 3-4 parts of sculellaria barbata, 3-4 parts of rheum officinale, 3-4 parts of golden cypress, 3-4 parts of fructus forsythiae, 3-4 parts of radix stemonae, 4-6 parts of polygonum cuspidatum, 4-6 parts of scutellaria baicalensis and 2-3 parts of liquorice. The preparation method of the Chinese medicine composite comprises the following steps: soaking the raw materials with 5-6 times by volume of water for 22-26 hours; decocting for 40-80min; filtering and separating medicine liquid from medicine dregs; adding water to the medicine dregs and decocting, filtering and separating the medicine liquid from medicine dregs; mixing the medicine liquid obtained in two decoction steps, standing for 22-26 hours, heating and concentrating to obtain an extract with specific gravity being 1.30-1.35; and adding dextrin into the extract, stirring uniformly, pelleting, drying at 60 DEG C and packaging finally. With the adoption of the Chinese medicine composite, the functions of preventing and treating diseases and enhancing immunity of bees are organically combined; and the Chinese medicine composite has the advantages of strong broad-spectrum antibacterial function, less side effects and high safety.
Description
One, technical field
The present invention relates to a kind of medicine and preparation method thereof, specifically a kind of sick Chinese medicine composition of honeybee and preparation method thereof of preventing and treating.
Two, background technology
At the beginning of 2002, some countries such as European Union and U.S.A, day borrow the interior chloromycetin content of Chinese Mel (European Union be decided to be the be no more than 0.10ppb) taboo that exceeds standard to advance and suspend sale of China's Mel, cause the Mel trade to be absorbed in low ebb, and economy receives heavy losses, and it is very big that the beekeeper takes in influence.
China reduces and pollutes in order to prevent that " medicine is residual " exceeds standard in the Mel, and antibiotic and some chemicals such as chloromycetin are ordered to ban use of by the Ministry of Agriculture, causes the sick medicine of usefulness that needs of control honeybee to reduce suddenly, brings the difficulty in the sick control of honeybee.Be challenge in the reply international trade and the difficulty of preventing and treating the honeybee disease, apiculture circle is tackled problems, and one of them key technology is the instant sick source new drugs of control honeybee of opening up, to replace antibiotic and the chemicals that is prone to pollution.
Characteristics according to the gregarious biological characteristics of Apis, the sick mixed infection frequently-occurring disease of honeybee; And the reality that endangers the most serious main infectious disease of Apis now; The new honeybee medicine of development will possess good, fast, the side Zhiduo County disease of producing effects of prevention effect; And do not pollute, do not produce " medicine is residual ", to performances such as Apis safety, and adapt to the beekeeper produce mobile big, the mode of production of many field works.New honeybee medicine must be easy to carry, be convenient to preserve and characteristics such as easy to use.
Three, summary of the invention
The present invention aims to provide a kind of sick Chinese medicine composition of honeybee and preparation method thereof of preventing and treating; Technical problem to be solved is to select suitable Chinese medicine preparation to make it have the effect of curative effect height, the multiple Apis infectious disease of control; And polluted product and environment do not substitute big antibiotic and the chemicals of the sick negative interaction of control honeybee.
Technical solution problem of the present invention adopts following technical scheme:
The present invention prevents and treats the sick Chinese medicine composition of honeybee, it is characterized in that its raw material constitutes by mass fraction:
Herba Scutellariae Barbatae 3-4 part, Radix Et Rhizoma Rhei 3-4 part, Cortex Phellodendri 3-4 part, Fructus Forsythiae 3-4 part, Radix Stemonae 3-4 part, Rhizoma Polygoni Cuspidati 4-6 part, Radix Scutellariae 4-6 part, Radix Glycyrrhizae 2-3 part.
The present invention prevents and treats the method for preparing of the sick Chinese medicine composition of honeybee, comprises immersions, decoction, filtration, concentrated, pelletize and each unit process of post processing, it is characterized in that:
Take by weighing each raw material by proportional quantity, the water logging that in raw material, adds 5-6 times of volume was steeped 22-26 hour, decocted 40-80min isolated by filtration medicinal liquid and medicinal residues; The decocting that in medicinal residues, adds 4 times of volumes boils 30-50min; Isolated by filtration medicinal liquid and medicinal residues merge filtered medicine liquid twice, leave standstill 22-26 hour; Heating concentrates and obtains the extractum that proportion is 1.30-1.35 (25 ℃) then; In said extractum, add dextrin, the back pelletize and of stirring in 60 ℃ of dry encapsulation, the addition of dextrin is the 50-100% of raw materials quality.
Every pouch 20g contains crude drug in whole 18-19g during encapsulation.
Chinese medicine composition composition granule of the present invention is little yellow or grey leucorrhea yellowish-brown, and stronger Chinese herbal medicine special odor is arranged, and is little sweet, the slightly bitter sense of aftertaste.Soluble in water, solution is brownish red, and is slightly transparent, pH value 5.5-6.0.
Chinese medicine composition composition granule pharmacological properties of the present invention is stable, and it is constant to preserve more than 2 years its matter under the room temperature, and if any a small amount of adhesion, its curative effect does not subtract.
The clinical method for using of Chinese medicine composition of the present invention:
One bag of Chinese medicine composition of the present invention (20g) is dissolved in the 50-60mL clear water, is used to spray spleen 22-26 and opens (about two groups of honeybees), also can be mixed with the medical syrup 300-400mL of sugar content 50%, every crowd of each 150-200mL that feed.Preventive administration 3-4 days 1 time, feed 2-3 time continuously; Therapeutic administration 2 days 1 time, feed continuously 3-5 time or more than.Join existing feeding, medicinal liquid can not be put for a long time, in order to avoid influence drug effect at present.(cryopreservation is better) preserved in the room temperature dark place, anti-Exposure to Sunlight, moisture proof.
[pharmacological effect]
The present invention is directed to the main communicate illness characteristics of Apis, the Chinese herbal medicine of optional bitter in the mouth in the Chinese medicine preparation, cold and heat-clearing and toxic substances removing, antiinflammatory, antibacterial functions is on the high side.8 flavors have in the Chinese herbal medicine 7 flavors to have the function of stronger heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, anti-inflammation detumescence, have 6 flavors to be rich in antibacterium, antiviral, and suppress some funguses, spiral shell substance, protozoon etc., and what have has a stronger lethal effect:
Herba Scutellariae Barbatae.Scutellaria barbata DDon. nature and flavor are hot, little hardship.Detoxify, invigorate blood circulation, antiinflammatory, detumescence, anti-multiple pathogen etc.
Radix Et Rhizoma Rhei.R·officinale?Baill。Nature and flavor are bitter, cold.Clearing away heat-fire is forgived the blood detoxifcation, blood circulation promoting and blood stasis dispelling, and anti-multiple malignant bacteria, virus suppress protozoacide etc.
Cortex Phellodendri.Pchinese Schneid (Cortex Phellodendri).Nature and flavor are bitter, cold.Heat-clearing and toxic substances removing, dampness, anti-multiple malignant bacteria, virus and some funguses, protozoon, leptospira etc.
Fructus Forsythiae.Forsythia?Suspense(Thunbr)Vahl。Nature and flavor are bitter, be slightly cold.Heat-clearing and toxic substances removing, antiinflammatory eliminating carbuncle eliminating stagnation, has a broad antifungal spectrum also all has certain inhibition or killing action to some viruses, fungus and leptospira etc.
The Radix Stemonae.Stemona?Sessilifolia(Miq)Franch.et.sav。Nature and flavor are sweet, bitter, tepor.Protozoon infusorian etc. there is stronger killing action, some antibacterials are had certain inhibitory action.
Rhizoma Polygoni Cuspidati.Polygonum?Cuspidatum?Sieb.et.zuec。Nature and flavor are bitter, cold.Heat-clearing and toxic substances removing, invigorate blood circulation, dampness removing, anti-various bacteria and virus have certain killing action to leptospira etc.
Radix Scutellariae.Scutellaria?Baicalensis?Greorgi。Nature and flavor are bitter, cold.Heat clearing and damp drying, removing pathogenic heat from blood and toxic substance from the body, antiinflammatory.Has a broad antifungal spectrum suppresses more than 10 kind of pathogenic bacterias such as Bacillus proteus.
Radix Glycyrrhizae.Glycyrrhiza?uralensis?Fisch。Nature and flavor are sweet, flat.The function that stronger detoxifcation, antiinflammatory, invigorating the spleen and replenishing QI are arranged.
The beneficial effect of Chinese medicine composition of the present invention is embodied in:
1, strong broad-spectrum antibacterial functions
Chinese medicine composition of the present invention is formed by 8 flavor Chinese herbal medicine compatibilities, shows pathogenic microorganism functions such as stronger, anti-various bacteria, virus, fungus, protozoon.Its pharmacological action possibly realize through following two approach and form.
The one,, efficiency factor is various in the Chinese herbal medicine, the material that is rich in stronger inhibition even kills and wounds cause of disease own.Chinese medicine composition solution of the present invention with 30% shows that in the laboratory test data its antibacterial circle diameter that suppresses many antibacterials such as Bacillus proteus, escherichia coli reaches 10 ~ 11d/mm.One of the composition that Chinese herbal medicine includes alkaloid (berberine, coptisine, 13-methyl-.psi.-coptisine. etc.); The effect that suppresses various bacteria, fungus and ameba is all arranged; In addition 8 flavor Chinese herbal medicine compatibilities, active ingredient is various, the pharmacological effect wide coverage; Anti-microbial pathogen not only function is strong, and kind is many.
Second approach of anti-microbial pathogen is that Chinese medicine of the present invention shows special pharmacodynamics effect especially.It is through regulating non-specific antibacterium, antiviral activity (cytophagy, bacteriocidin, bacteriolysin etc.) and agents enhance overall immunity in the body, suppresses and strangles pathogenic microorganism.Many Chinese herbal medicine with heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, anti-inflammation detumescence, the effect of external antibacterium, virus is not too obvious, but sick clinically the control honeybee, shows comparatively ideal curative effect.This is Chinese medicine curative effect of the present invention intrinsic factor preferably.
2, has multi-functional and wider pharmacological action
Very complicated based on medicinal herb components, add 8 flavor Chinese herbal medicine compatibilities, make composition comprehensive and abundant more; Function is many; Pharmacological action is wider, and this is basis and advantage that Chinese medicine of the present invention can " a side Zhiduo County sick ", be antibiotic and chemicals the most outstanding characteristics that can not reach.
The present invention 8 flavor Chinese herbal medicine formulas are pharmacology " mutual reinforcement between compatibility " of taking effect identical or approaching, show preferable collaborative, stack, potentiation clinically, strengthen pharmacodynamics effect greatly, have promoted the sick curative effect of control honeybee.
3, organically combine one to disease preventing and treating and enhancing honeybee body immunity
Chinese medicine composition of the present invention is through strengthening vital QI to eliminate pathogenic factors, equilibrium between yin and yang; Improve the Apis immunity of organisms; Starting with from regulating allomeric function, add collaborative, superposition in the treatment, is Chinese medicine composition of the present invention is obtained significant curative effect on slight Apis body outstanding feature." a side Zhiduo County is sick " of Chinese medicine composition of the present invention meets Chinese medicine " many sick sides " treatment principle.
4, few side effects, safe
Show that at first Chinese medicine composition control honeybee of the present invention is sick, to beneficial microbe group side effect in the body seldom, protected the defense system of useful bacterium colony, exempt antibiotic and chemicals harm beneficial microorganism drawback body.Using Chinese medicine composition of the present invention can not produce " medicine is residual ", Drug resistance and some drug-induced disease basically in addition, also is the event of another kind of intrinsic factor that Chinese medicine composition control honeybee disease of the present invention obtains more satisfactory curative effect.
2003-2004 is through nearly 10 toxicity tests, and 2004 with later clinical practice, confirm that all Chinese medicine composition of the present invention is grown Apis safety, queen to lay eggs, young honeybee and to become honeybee to interview all normal, do not find any abnormal phenomena.Accept the sick Apis of medical treatment honeybee, Chinese medicine composition high safety of the present invention all is described for 1145 groups.
Four, the specific embodiment
The raw material of Chinese medicine composition of the present invention constitutes by mass fraction:
3.5 parts of Herba Scutellariae Barbataes, 3.5 parts of Radix Et Rhizoma Rhei, 3.5 parts of Cortex Phellodendris, 3.5 parts of Fructus Forsythiaes, 3.5 parts of the Radixs Stemonae, 5.0 parts of Rhizoma Polygoni Cuspidati, 5.0 parts of Radix Scutellariaes, 2.5 parts in Radix Glycyrrhizae.
Method for preparing:
Take by weighing each raw material by proportional quantity, the water logging that in raw material, adds 5-6 times of volume was steeped 22-26 hour, decocted 40-80min isolated by filtration medicinal liquid and medicinal residues; The decocting that in medicinal residues, adds 4 times of volumes boils 30-50min, and isolated by filtration medicinal liquid and medicinal residues merge filtered medicine liquid twice; Left standstill 22-26 hour; Heating concentrates and to obtain the extractum that proportion is 1.30-1.35 (25 ℃) then, in said extractum, adds the dextrin with raw material equivalent, and back pelletize and in 60 ℃ of dry encapsulation stirs.Every pouch 20g contains crude drug 18-19g during encapsulation.
The particulate trade name of Chinese medicine composition of the present invention is " a honeybee children health ".
Method for using:
One bag of honeybee children's health (20g) is dissolved in the 50-60mL clear water, is used to spray spleen 22-26 and opens (about two groups of honeybees), also can be mixed with the medical syrup 300-400mL (20g honeybee children health) of sugar content 50%, every crowd of each 150-200mL that feed.Preventive administration 3-4 days 1 time, feed 2-3 time continuously; Therapeutic administration 2 days 1 time, feed continuously 3-5 time or more than.Join existing feeding, medicinal liquid can not be put for a long time, in order to avoid influence drug effect at present.
[" honeybee children health " clinical effectiveness]
The sick result of the control honeybee in 2004 ~ 2007 4 years proves evident in efficacy.
1, preventive administration effect
Select 649 groups in 2003,2004 ill bee colonies, 2005 with " honeybee children health " prevention, 2005 to 2006 former harm do not take place every year infectious disease, preventive effect 100%.
2, therapeutic administering effect
1095 groups of 10 counties (city) such as Xuancheng, Anhui Province, Guangde treatments beekeeper's the ill Apiss of 39 bee farms, total effective rate 98.01%, wherein cure rate 74.29%, obvious effective rate 14.17%, effective percentage 9.55%, inefficiency is merely 1.99%.
In therapeutic process, the European foul brood that runs into and treat, the sick therapeutic effect of honeybees such as American foul brood, Apis bee paralysis and bee spirochaetosis syndrome is best, and the ascospherosis of bee curative effect is taken second place.The Apis amoebiasis only run into 11 groups of honeybee morbidities of 1 bee farm, and curative effect is better, because of receiving treatment the disease crowd very little, remain further to be studied.Several kinds of main infectious disease therapeutic effect records of Apis see the following form.
" honeybee children health " sick effect recording of treatment honeybee
3, curative effect for example
Case l, European foul brood
160 groups of the township beekeeper Sun Hao bee-keeping that face south of Xuancheng City, Anhui Province; In April, 2005,42 groups of honeybee elder generation future trouble European foul broods, the large tracts of land larva is ill, rancid death, the polypide atrophy is withered, occurs " beggar's spleen ".Be made into medical syrup with " honeybee children health " and feed, medication in 2-3 days 1 time, two back 42 groups of healings of week.
Contain new people and raise bees 100 groups in this township, on May 10th, 2004, have 30 groups ill, be made into the treatment of medicinal liquid spray spleen with " honeybee children health " May 15 and recover after 10 days or cure basically.
Case 2, bee spirochaetosis
60 groups of the strong bee-keeping of Anhui Xiao Xian Li Fan, on April 20th, 2006, the crowd suffered from bee spirochaetosis, and adult honeybee constantly climbs out of, and can not fly up, and creeping in ground, dies of exhaustion soon, and crowd's gesture descends rapidly.Be made into medical syrup with " honeybee children health " and feed honeybee, fed 1 time in 2 days, each 500-600ml, 20 groups of sick honeybees are fully recovered successively after 10 days.110 groups of town beekeeper Chen Shouquan bee-keeping are scooped up in the Feidong County, Anhui, March in 2005 30 groups of performance bee spirochaetosis symptoms are arranged, and with " honeybee children health ", after the treatment, symptom alleviated until disappearance gradually in 4-5 days.Recover 8 groups, 13 groups of produce effects, effective 9 groups have effectively been controlled PD.
Case 3, ascospherosis of bee
Xiuzhou City, Anhui Province beekeeper Li Bingshu, Yushan, business section, Chuzhou is raised bees 120 groups and 60 groups respectively.Ascospherosis of bee successively appears in June, 2005, and the bee larvae flavescence is brown and dead, and polypide is drying shrinkage gradually, and is stiff, becomes white lime color or brown lump.With " honeybee children health " the spray spleen or the treatment of feeding, the 38 groups of sick honeybees healings are 18 groups after 20 days,, effective 20 groups, the state of an illness is controlled.
Case 4, Apis bee paralysis
Huizhou District Xi Xi South Asia, Huangshan, Anhui Province beekeeper Ruan Guangming raises bees 100 groups, and in February, 2004,20 groups of trouble " Apis bee paralysis " cause large quantities of adult honeybees dead, have a strong impact on bee product production.Sick honeybee wing opens, can not fly, and the glossy blackout of body, the performance abdominal part that has expands (bulky belly), is slow in action, and bradykinesia, metapedes are the paralysis shape, the difficulty of creeping, what have falls within the bottom of the case, dies at last.Feed honeybee continuously with " honeybee children health " syrup, recover 2 groups of produce effects, effective percentage 100% for 18 groups.
Can find out that from above data the present invention's " honeybee children health " clinical application effect shows to have outstanding curative effect characteristics and clear superiority.At first; " honeybee children health " with the Chinese herbal medicine development; Replace several kinds of main infectious disease of antibiotic and chemicals control Apis, this prevents and treats on the history in the honeybee disease, can be described as a bigger breakthrough; Finish to rely on antibiotic and the sick situation of chemicals control honeybee, for apiculture produces bigger economic worth and benefit.
" a side Zhiduo County is sick " has multi-functional control honeybee characteristic of disease ability, and ' this is " honeybee children health " the most outstanding characteristics.Clinical practice proves: the American foul brood that pair cell causes, European foul brood; The Apis bee paralysis that virus causes, Apis sac brood; " bee spirochaetosis " syndrome that spiral shell substance, full sub-worm etc. cause; And the Apis amoebiasis that cause of fungus-caused ascospherosis of bee, Malpighian tube amebicide, significant curative effect or better curative effect are all arranged.Succeeding in developing of " honeybee children health ", limitation (side controls a disease) that its change past Chinese herbal medicine is cured the disease and the hysteresis quality on the treatment honeybee disease.
" mutual reinforcement between compatibility ", the curative effect stack.This is " honeybee children health " the 3rd outstanding feature and advantage.In " honeybee children health " prescription, the Chinese herbal medicine pharmacological effect of large percentage roughly the same or close, treatment honeybee disease shows out collaborative, synergic superposition especially.Add treatment and improve honeybee body immunity and integrally combine the curative effect ideal.
Reducing and pollute, stop " medicine is residual ", is " honeybee children health " another important value.Apply " honeybee children health "; Replaced antibiotic and chemicals, reduced pollution, stopped " medicine residual " quality and the safety of assurance bee product bee product and environment; Improve Competition in International Trade power greatly; Can prevent effectively that European Union in 2002 from borrowing Mel to contain the chloromycetin amount and exceeding standard, prohibit to advance and stop to sell similar incidents such as China's Mel protection apiculture and beekeeper's interests take place.
" honeybee children health " and other Chinese herbal and crude drugs preparations; Replace antibiotic and chemicals; Get into the sick prevention and control field of honeybee, not only can changing in the past, bee-keeping relies on antibiotic and chemicals, the not situation of science medication; And realize standardized production and obtain the target of green bee product, make China's apiculture stride forward the sick new period of non-environmental pollution control honeybee.
In addition, " honeybee children health " is easy to use, the medication science.Seldom used the herbal control honeybee sick in the past, and promptly be to use and take decocting method acquisition decoction to cure the disease, and increased much troubles, particularly beekeeper's field mobile operation is more difficult.Thick medicine purified " honeybee children health " dissolved granule, 20 grams, one bag, speed is water-soluble, and spray spleen or feed is all suitablely used the dose easy master.Be easy to carry again, be convenient to preserve, satisfied beekeeper's requirement.
Claims (2)
1. prevent and treat the sick Chinese medicine composition of honeybee for one kind, it is characterized in that its raw material constitutes by mass fraction:
Herba Scutellariae Barbatae 3-4 part, Radix Et Rhizoma Rhei 3-4 part, Cortex Phellodendri 3-4 part, Fructus Forsythiae 3-4 part, Radix Stemonae 3-4 part, Rhizoma Polygoni Cuspidati 4-6 part, Radix Scutellariae 4-6 part, Radix Glycyrrhizae 2-3 part.
2. the method for preparing of the Chinese medicine composition of the described control of claim 1 a honeybee disease is characterized in that
Take by weighing each raw material by proportional quantity, the water logging that in raw material, adds 5-6 times of volume was steeped 22-26 hour, decocted 40-80min isolated by filtration medicinal liquid and medicinal residues; The decocting that in medicinal residues, adds 4 times of volumes boils 30-50min, and isolated by filtration medicinal liquid and medicinal residues merge filtered medicine liquid twice; Left standstill 22-26 hour; Heating concentrates and to obtain the extractum that proportion is 1.30-1.35 (25 ℃) then, in said extractum, adds dextrin, and back pelletize and in 60 ℃ of dry encapsulation stirs; The addition of dextrin is the 50-100% of raw materials quality.
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| CN104127505A (en) * | 2014-07-06 | 2014-11-05 | 湖北天吉蜜业科技有限公司 | Chalkbrood eliminating granule Chinese patent medicine for treating bee chalkbrood disease and production technology thereof |
| CN108815286A (en) * | 2018-09-05 | 2018-11-16 | 博罗县柏塘丰养蜂专业合作社 | It is a kind of to prevent and treat sick Chinese herbal and crude drugs preparations of the rotten son of honeybee and preparation method thereof and method of administration |
| CN110638871A (en) * | 2019-10-12 | 2020-01-03 | 中国农业科学院蜜蜂研究所 | Anti-bee paralysis virus extract and preparation method thereof |
| CN112843153A (en) * | 2021-03-22 | 2021-05-28 | 达州市农业科学研究院 | Nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity, and preparation method and application thereof |
| CN113197950A (en) * | 2021-04-22 | 2021-08-03 | 吴学刚 | Traditional Chinese medicine prescription for treating European rotten seed disease and sacbrood disease |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104127505A (en) * | 2014-07-06 | 2014-11-05 | 湖北天吉蜜业科技有限公司 | Chalkbrood eliminating granule Chinese patent medicine for treating bee chalkbrood disease and production technology thereof |
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| CN108815286B (en) * | 2018-09-05 | 2021-05-25 | 博罗县柏塘丰养蜂专业合作社 | Chinese herbal medicine preparation for preventing and treating bee brood and preparation method and application method thereof |
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| CN110638871B (en) * | 2019-10-12 | 2021-07-13 | 中国农业科学院蜜蜂研究所 | Anti-bee paralysis virus extract and preparation method thereof |
| CN112843153A (en) * | 2021-03-22 | 2021-05-28 | 达州市农业科学研究院 | Nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity, and preparation method and application thereof |
| CN113197950A (en) * | 2021-04-22 | 2021-08-03 | 吴学刚 | Traditional Chinese medicine prescription for treating European rotten seed disease and sacbrood disease |
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Application publication date: 20121226 |