CN108815286B

CN108815286B - Chinese herbal medicine preparation for preventing and treating bee brood and preparation method and application method thereof

Info

Publication number: CN108815286B
Application number: CN201811030285.8A
Authority: CN
Inventors: 陈海塘
Original assignee: Boluo Baitangfeng Beekeeping Professional Cooperative
Current assignee: Boluo Baitangfeng Beekeeping Professional Cooperative
Priority date: 2018-09-05
Filing date: 2018-09-05
Publication date: 2021-05-25
Anticipated expiration: 2038-09-05
Also published as: CN108815286A

Abstract

The invention discloses a Chinese herbal medicine preparation for preventing and treating bee rotten son disease and a preparation method and an application method thereof, wherein the raw materials of the Chinese herbal medicine preparation comprise 20-30 parts of rhizoma corydalis; 15-20 parts of barbed skullcap herb; 10-15 parts of sweet wormwood herb; 8-12 parts of honeysuckle; 8-12 parts of phellodendron; 8-12 parts of astragalus; 8-12 parts of desmodium; 8-12 parts of liquorice; and 30-40 parts of honey. The honey is used for soaking the effective components of the Chinese herbal medicines, the honey is eaten by sick bee colonies, the bee rotten son disease is effectively treated, the effective components in the Chinese herbal medicines can be stored for a long time, no medicine residue is left on bee products, and the safety requirement of pollution-free food production is met.

Description

Chinese herbal medicine preparation for preventing and treating bee brood and preparation method and application method thereof

Technical Field

The invention relates to the field of bee breeding, in particular to a Chinese herbal medicine preparation for preventing and treating bee brood, a preparation method and a use method thereof.

Background

Bee brood is a bee viral infectious disease which is commonly generated in the world, is caused by the bacillus larvae, is bred in rotten larvae, and is spread by nurturing other larvae by worker bees. The bee colony is weakened day by day due to strong infection and high death rate of pathogenic bacteria until the bee colony flies away or dies. Causing destructive loss, no specific medicine is radically cured at present, and antibiotic medicines such as chloramphenicol and the like are generally adopted for treatment. Antibiotics can not be decomposed by self and remain in bee products, so that the problem of food safety is increasingly serious.

Disclosure of Invention

Because the prior art mostly uses antibiotics to treat the rotten son disease, the antibiotics are remained in honey and other products, so that the honey and bee products do not meet the food safety requirements, and the food safety problem is caused.

The invention aims at the technical problems and provides a Chinese herbal medicine preparation for preventing and treating bee brood, a preparation method and an application method thereof.

The invention aims to solve the technical problems and provides a Chinese herbal medicine preparation for preventing and treating bee rotten son disease, which comprises raw materials of rhizoma corydalis, barbed skullcap herb, sweet wormwood herb, honeysuckle flower, amur corktree bark, astragalus root, longhairy antenoron herb, liquorice and honey.

Further, 20-30 parts of rhizoma corydalis; 15-20 parts of barbed skullcap herb; 10-15 parts of sweet wormwood herb; 8-12 parts of honeysuckle; 8-12 parts of phellodendron; 8-12 parts of astragalus; 8-12 parts of desmodium; 8-12 parts of liquorice; and 30-40 parts of honey.

Specifically, the Chinese herbal medicine preparation for preventing and treating bee rotten son disease is prepared by crushing and mixing rhizoma corydalis, barbed skullcap herb, sweet wormwood herb, honeysuckle flower, golden cypress, astragalus mongholicus, desmodium and liquorice root, and soaking the mixture in honey to prepare a paste.

Rhizoma corydalis, also known as corydalis tuber and corydalis tuber, is a perennial herb of corydalis genus of Papaveraceae. Corydalis Tuber is recorded in KAI BAO Ben Cao, warm in nature, pungent and bitter in flavor, entering heart, spleen, liver and lung. Corydalis tuber contains various alkaloids: d-corydaline (d-corydaline), dl-tetrahydropalmatine (dltetrahydropalmatine), protopine (protopine), l-tetrahydrocoptisine (1-tetrahydrocoptisine), d 1-tetrahydrocoptisine (dltetrahydrocoptisine), coptisine (coptisine), and the like.

Barbat skullcap, academic name: scutellaria barbata D.Don (plant material of Jiangsu province), which is called as: stringy exterior syndrome (Sichuan Chengdu), thin baikal skullcap root (Sichuan Puge), tooth-brush grass (Jiangsu Suzhou), Tianji grass (Jiangsu Yixing), Shuichu baikal skullcap root (Jiangsu Nanjing), and Han Xin grass (Guangzhou plant). Is a perennial herb of the genus Scutellaria of the family Labiatae. The herba Scutellariae Barbatae has effects in clearing away heat and toxic materials, promoting blood circulation, dispelling blood stasis, relieving swelling and pain, and resisting cancer. Is cold in nature and sour in taste, and the whole herb contains various vitamins, trace elements, amino acids and other components. Has the effects of cooling blood, removing toxic substances, removing blood stasis, relieving pain, relieving swelling, clearing heat and promoting diuresis. The barbed skullcap herb contains: carthamin (carthamidin), isocarthamin (iso-carthamidin), scutellarin (scutellarein), kainic (scutellarin-larin), beta-sitosterol (beta-sitosterol), stearic acid (stearic acid), alkaloid polysaccharide, and the like.

Artemisia annua (Artemisia carvifolia) is a annually grown herb. The stem is upright, the upper part is multi-branched and has a longitudinal ridge line. Leaves grow with each other, leaves in the middle of the stem return to pinnate division, and linear lobes. In summer, the head-shaped inflorescence is hemispherical, mostly conical, tubular, female outside and amphoteric inside. The sweet wormwood has the function of resisting pathogenic microorganisms: the decoction of herba Artemisiae Annuae has effect in inhibiting various bacteria. The sweet wormwood contains the following chemical components: sesquiterpene lactones: artemisinin compounds such as artemisinin, artesunic acid A, artesunic acid B, and artesunic lactone. And (3) flavonoids: kaempferol (kaempferol), quercetin.

Honeysuckle, known as Lonicera japonica Thunb. Honeysuckle is named from compendium of materia Medica, and is obtained because honeysuckle flowers turn white at first and then turn yellow. The flos Lonicerae is dried bud or flower with initial blossom of Lonicera Japonica of Lonicera of Caprifoliaceae and plants of the same genus. Honeysuckle is known as a good medicine for clearing heat and removing toxicity from old times. It is sweet and cold in nature and fragrant, sweet and cold in nature and clearing heat without hurting stomach, and the fragrance is thorough and can eliminate pathogens. Honeysuckle flower can disperse wind-heat and clear blood toxin.

Phellodendron amurense, a dry bark of Phellodendron chinense schneid, a plant of the rutaceae family. It is commonly called Chuan Huang Bai. Peeling the bark, removing the coarse bark, and drying in the sun. Has effects of clearing heat, eliminating dampness, purging pathogenic fire, removing steam, removing toxic substance, and treating sore.

Radix astragali is root of Astragalus membranaceus bge of Leguminosae. Digging in spring and autumn, removing soil, fibrous root and root head, drying in the sun to six or seven percent, straightening, bundling and drying in the sun. The main functional indications are as follows: tonify qi, strengthen superficies, expel toxin, expel pus, induce diuresis, and promote granulation. Can be used for treating deficiency of vital energy, asthenia, chronic diarrhea, proctoptosis, spontaneous perspiration, edema, prolapse of nasal mucosa and nasal mucosa, chronic nephritis, albuminuria, diabetes, and unhealed wound. Contains calycosin (calycosin) and 3-hydroxy-9, 10-dimethoxy rosewood alkane as flavone components, and also contains astragalosides I, V and III.

Lysimachia christinae, latin literature name Lysimachia christinae Hance, alias: lysimachia christinae hance, specular grass, aleppo stephania, lotus bud grass, meat wonton grass, gold key, longtube ground ivy herb, sedum sarmentosum, lysimachia christinae hance, qian ye grass, qian jin and the like. Is a variety of plants which are called as creeping small herbs in many years of angiosperms, tubular florales, primula families and pearl vegetable genera. The leaf kidney is shaped to be round, the diameter is 4-25 mm, the flower is single-grown, the axilla is axillary, the flower stalk is shorter than the leaf stalk, and the flower stalk is filiform; green leaves, yellow florets. The longhairy antenoron herb contains flavonoid components: quercetin (quercetin), isoquercitrin, i.e., quercetin-3-O-glucoside (isoquercitrin, quercetin-3-O-gluco-side), kaempferol (kaempferol), trifolioside, i.e., kaempferol-3-O-galactoside (trifolin, kaempferol-3-O-galactosides), p-hydroxybenzoic acid (p-hydroxy benzoic acid), uracil (uridine), sodium chloride; potassium chloride, nitrite, cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) like substances, polysaccharide and 9 elements such as calcium, magnesium, iron, zinc, copper, manganese, cadmium, nickel, cobalt and the like.

RADIX Glycyrrhizae Preparata (RADIX Glycyrrhizae Preparata) is prepared by decocting RADIX Glycyrrhizae baked with honey in a pan, parching with slow fire, adding sliced RADIX Glycyrrhizae, parching uniformly for 3-5 min, baking in a baking room or oven 60-C until it is not sticky, and cooling. The honey-fried licorice root is a round-like or oval slice, the surface is reddish brown or grayish brown, the honey-fried licorice root is slightly glossy, the section is yellow to dark yellow, the cambium ring is obvious, and the rays are radial. The radix Glycyrrhizae Preparata contains glycyrrhizin, glycyrrhetinic acid, and Glycyrrhiza polysaccharide, and has antiinflammatory, antiallergic, immunity regulating, antitumor, and analgesic effects.

Compared with the prior art, the method has the following positive effects:

1. the Chinese herbal medicine preparation for preventing and treating bee rotten son disease provided by the invention is prepared by crushing the Chinese herbal medicines according to a ratio by utilizing the life habit that bees like to eat honey and skillfully utilizing the technology of refining honey into pills by ancient people, mixing the crushed Chinese herbal medicines with honey into paste, soaking the effective components of the Chinese herbal medicines by using honey, and eating the effective components for sick bee colonies, so that the bee rotten son disease is effectively treated, the effective components in the Chinese herbal medicines can be stored for a long time, no medicine residue is left on bee products, and the safety requirement of production of pollution-free food is met;

2. the Chinese herbal medicine preparation utilizes pure natural Chinese herbal medicines and honey, has no chemical composition and antibiotic components, has no residue and no public nuisance, effectively inhibits the development and propagation of pathogenic bacteria, and solves the problems that the root breaking is difficult, the root breaking is easy to relapse, the antibiotic remains in bee products and the like when chemical antibiotic medicines are applied to treat bee rot in the prior art;

3. the Chinese herbal medicine preparation of the invention effectively enhances the immunity of bee colonies;

4. the Chinese herbal medicine preparation uses natural environment-friendly raw materials, is green and environment-friendly, and ensures the safety and health of eaters.

The invention also provides a preparation method of the Chinese herbal medicine preparation for preventing and treating bee rotten son disease, which comprises the following steps:

1) selecting the following raw materials in parts by weight: 20-30 parts of rhizoma corydalis, 15-20 parts of sculellaria barbata, 10-15 parts of sweet wormwood herb, 8-12 parts of honeysuckle flower, 8-12 parts of golden cypress, 8-12 parts of astragalus membranaceus, 8-12 parts of desmodium, 8-12 parts of liquorice and 30-40 parts of honey;

2) cleaning rhizoma corydalis, herba Scutellariae Barbatae, herba Artemisiae Annuae, flos Lonicerae, cortex Phellodendri, radix astragali, herba Lysimachiae Christinae and Glycyrrhrizae radix, drying, and pulverizing;

3) mixing the pulverized rhizoma corydalis, herba Scutellariae Barbatae, herba Artemisiae Annuae, flos Lonicerae, cortex Phellodendri, radix astragali, herba Lysimachiae Christinae and Glycyrrhrizae radix, adding into the Mel, soaking, and concocting into paste.

Wherein the rhizoma corydalis, the barbed skullcap herb, the sweet wormwood herb, the honeysuckle, the amur corktree bark, the astragalus root, the desmodium and the liquorice are crushed into 100 meshes.

Compared with the prior art, the preparation method of the Chinese herbal medicine preparation for preventing and treating bee rotten son disease is simple and convenient, uses natural environment-friendly raw materials, is green and environment-friendly, and ensures safety and health of eaters.

Another technical scheme for solving the technical problems of the invention is that an application method of a Chinese herbal medicine preparation for preventing and treating bee brood disease for treating bee brood disease comprises the following steps: the Chinese herbal medicine preparation for bee brood is put on the upper beam of the nest of the sick bee colony, the amount of each bee frame corresponding to the Chinese herbal medicine preparation is 5g, the feed is carried out for 1 time every other day, and the feed is continuously carried out for 3-5 times.

The other technical scheme for solving the technical problems of the invention is that the application method of the Chinese herbal medicine preparation for preventing and treating the bee brood disease, which is used for preventing the bee brood disease, comprises the following steps: the Chinese herbal medicine preparation for bee brood is put on the upper beam of the hive frame of the bee colony, the amount of each frame of bees corresponding to the Chinese herbal medicine preparation is 5g, and the Chinese herbal medicine preparation is used for 1 time in one month.

Compared with the prior art, the application method of the Chinese herbal medicine preparation for preventing and treating bee rotten son disease is simple and convenient, uses natural environment-friendly raw materials, is green and environment-friendly, and ensures safety and health of eaters.

The invention is further illustrated by the following figures and examples.

Drawings

Fig. 1 is a process for preparing the Chinese herbal medicine preparation for preventing and treating bee brood in the first embodiment.

Detailed Description

Example one

As shown in fig. 1, the Chinese herbal medicine preparation for preventing and treating bee rotten son disease of the first embodiment is prepared by the following steps:

1) selecting raw materials: 30g of rhizoma corydalis, 20g of sculellaria barbata, 15g of sweet wormwood herb, 12g of honeysuckle flower, 8g of golden cypress, 12g of astragalus membranaceus, 12g of desmodium, 8g of liquorice and 40g of honey;

2) the Chinese herbal medicine raw materials are: the rhizoma corydalis, the barbed skullcap herb, the sweet wormwood herb, the honeysuckle flower, the amur corktree bark, the astragalus root, the desmodium and the liquorice are cleaned, dried and crushed into 100 meshes;

3) mixing the pulverized rhizoma corydalis, herba Scutellariae Barbatae, herba Artemisiae Annuae, flos Lonicerae, cortex Phellodendri, radix astragali, herba Lysimachiae Christinae and Glycyrrhrizae radix, adding into the Mel, stirring, soaking, and concocting into paste;

4) and (5) subpackaging for later use after the inspection is qualified.

When the Chinese herbal medicine preparation for preventing and treating bee brood disease in the first embodiment is used for treating bee brood disease, the Chinese herbal medicine preparation in the first embodiment is placed on the upper beam of a hive frame of a sick bee colony, the amount of each frame of bee corresponding to the Chinese herbal medicine preparation in the first embodiment is 5g, and the feeding is carried out for 1 time every other day and is carried out for 3-5 times continuously.

When the Chinese herbal medicine preparation for preventing bee brood in the first embodiment is used for preventing bee brood, the Chinese herbal medicine preparation in the first embodiment is placed on the upper beam of the hive frame of a bee colony, the amount of each frame of bee corresponding to the Chinese herbal medicine preparation in the first embodiment is 5g, and the Chinese herbal medicine preparation is used for 1 time in a month.

Example two

The Chinese herbal medicine preparation for preventing and treating bee brood of the second embodiment comprises the following raw materials: 20g of rhizoma corydalis, 15g of barbed skullcap herb, 10g of sweet wormwood herb, 8g of honeysuckle flower, 12g of golden cypress, 8g of astragalus root, 8g of desmodium, 12g of liquorice and 30g of honey.

The manufacturing method of the second embodiment is the same as that of the first embodiment.

The method of administration of the rotten son's disease treatment of example two is the same as that of example one.

The method of administration for the prophylaxis of rotten son's disease of example two is the same as that of example one.

EXAMPLE III

The Chinese herbal medicine preparation for preventing and treating bee brood in the third embodiment comprises the following raw materials: 24g of rhizoma corydalis, 18g of barbed skullcap herb, 12g of sweet wormwood herb, 11g of honeysuckle flower, 10g of golden cypress, 9g of astragalus root, 11g of desmodium, 9g of liquorice and 33g of honey.

The manufacturing method of the third embodiment is the same as that of the first embodiment.

The method of administration of the treatment of rotten son's disease of example three is the same as that of example one.

The method of administration for the prophylaxis of rotten son's disease in example three is the same as in example one.

Example four

The Chinese herbal medicine preparation for preventing and treating bee brood in the fourth embodiment comprises the following raw materials: 28g of rhizoma corydalis, 16g of barbed skullcap herb, 14g of sweet wormwood herb, 9g of honeysuckle flower, 9g of golden cypress, 11g of astragalus mongholicus, 9g of desmodium, 11g of liquorice and 38g of honey.

The manufacturing method of the fourth embodiment is the same as that of the first embodiment.

The method of administration of the rotten son's disease treatment of example four is the same as that of example one.

The method of administration for the prophylaxis of rotten son's disease of example four is the same as that of example one.

Comparative test

The Chinese herbal medicine preparation for preventing and treating bee brood in the first embodiment is adopted for comparison test.

Test No.)

And (5) performing an effect test for treating bee brood.

The Chinese bees suffering from the rotten son disease are divided into three groups according to the number of the bees, and the groups are far enough from each other so as not to be infected mutually.

Example set: the first group of bee colonies are fed with the Chinese herbal medicine preparation for preventing and treating bee brood disease of the first embodiment, and the feeding method comprises the following steps: the Chinese herbal medicine preparation of the first embodiment is placed on the upper beam of the hive frame of the sick bee colony, the amount of each frame of bees corresponding to the Chinese herbal medicine preparation of the first embodiment is 5g, and the feeding is carried out for 1 time every other day and for 3-5 times continuously.

Comparative example group: the second group of bee colony is fed with chloramphenicol by the following feeding method: the chloramphenicol is added into white sugar water to attract bees to eat. 100 ten thousand units of chloramphenicol were fed to 20 swarm bees.

Natural control group: the third group of swarms did not receive any drug.

After continuously observing for 15 days, the death number of worker bees is recorded, and the statistical data of the table 1 are obtained.

TABLE 1

As can be seen from Table 1, the number of deaths in the three groups was comparable at the beginning of the drug administration (day 2 of the test), indicating that no drug effect was apparent.

In the middle stage of drug use (test day 5, test day 10), the drug effects of the example group and the comparative example group are shown, the death number of the example group is reduced from 80 to 25, and the drug effects are excellent and remarkably effective compared with the comparative example group and the natural control group. The number of deaths of the comparative example group was slightly reduced, but by the 10 th day of the test, the number of deaths increased again, indicating that chloramphenicol was used, and the symptoms were treated but not the root causes, and the affected bee colony developed drug resistance.

By the end of the drug application (day 15 of the trial), no dead worker bees were produced in the example group, indicating a complete cure of the rotten son disease of the colony. The Chinese herbal medicine preparation of the invention has fast and good curative effect. Compared with the comparative example group, the death number of the comparative example group is greatly increased, the death number of the comparative example group is 3/5 of the natural control group, and the treatment effect is poor.

The data in the example group show that from day 2 of the test, the number of worker bees died continues to decrease without rebound, and by day 10, the number of died is the first 1/8, indicating that the threat of the rotten son disease to the diseased bee colony has been relieved, and from treatment to cure, only day 15, the faster the rotten son disease is treated, the better the rotten son disease is preserved, the loss is saved, and the cost is reduced.

In conclusion, the Chinese herbal medicine preparation for treating bee rotten son disease has the advantages of quick response, stable and continuous drug effect and short curing time, adopts natural environment-friendly raw materials, has no drug residue on bee products, and meets the production safety requirement of pollution-free food.

Test No. two

And (5) performing an effect test for preventing bee brood.

Three healthy Chinese bee fields are selected and divided into three groups, and the groups have enough distance without mutual infection.

Example set: the bee colony of the first group of bee farms comprises 55 bee colonies, and the bee colonies are fed with the Chinese herbal medicine preparation for preventing and treating bee brood diseases in the embodiment I, and the feeding method comprises the following steps: the Chinese herbal medicine preparation of the first embodiment is placed on the upper beam of the hive frame of the bee colony, the amount of each frame of bees corresponding to the Chinese herbal medicine preparation of the first embodiment is 5g, and the Chinese herbal medicine preparation is used for 1 time in one month.

Comparison group: the bee field of the second group has 60 bee colonies, and the bee colonies are fed with chloramphenicol by the feeding method: the chloramphenicol is added into white sugar water to attract bees to eat. 100 ten thousand units of chloramphenicol were fed to 20 swarm bees.

Control group: the third group of bee farms had 50 colonies, which were not fed any drug, as a natural control.

After continuously observing for 15 days, the number of the bee colonies suffering from the rotten son disease is recorded, and the statistical data in the table 2 are obtained.

TABLE 1

As can be seen from Table 2, only one diseased colony appeared in the control group at the initial stage of the test (test day 2), indicating that both the herbal formulation of example one and chloramphenicol of comparative example have the effect of preventing the rotten son disease.

In the middle period of the test (test day 5, test day 10), the diseased bee colonies of the control group gradually expanded and were difficult to control, and the control group also produced a small amount of diseased bee colonies and had an increasing trend, indicating that chloramphenicol was not effective in suppressing the occurrence of the rotten son disease. By contrast, the group of examples still did not show diseased bee colonies, indicating that the herbal formulation of example one can provide a sustained and effective prophylaxis against rotten son's disease.

By the end of the test (day 15 of the test), the example group still has no diseased bee colony, which shows that the Chinese herbal medicine preparation of the first embodiment can continuously and effectively prevent the rotten son disease. In contrast to the control group, the diseased bee colonies have risen dramatically to 10 and 23 colonies, respectively, indicating that chloramphenicol is not effective in preventing and curing the diseased bee colonies.

In conclusion, the Chinese herbal medicine preparation provided by the invention is used for preventing bee rotten son disease, the medicine effect is continuous and stable, and the natural environment-friendly raw materials are adopted, so that the Chinese herbal medicine preparation has no medicine residue on bee products, and meets the safety requirements of pollution-free food production.

The present invention is not limited to the above-described embodiments, and various changes and modifications of the present invention are intended to be included within the scope of the claims and the equivalent technology of the present invention if they do not depart from the spirit and scope of the present invention.

Claims

1. A Chinese herbal medicine preparation for preventing and treating bee rotten son disease is characterized in that: the raw materials comprise rhizoma corydalis, herba Scutellariae Barbatae, herba Artemisiae Annuae, flos Lonicerae, cortex Phellodendri, radix astragali, herba Lysimachiae Christinae, Glycyrrhrizae radix and Mel;

the raw materials comprise, by weight, 20-30 parts of rhizoma corydalis; 15-20 parts of barbed skullcap herb; 10-15 parts of sweet wormwood herb;

8-12 parts of honeysuckle; 8-12 parts of phellodendron; 8-12 parts of astragalus; 8-12 parts of desmodium; 8-12 parts of liquorice; and 30-40 parts of honey.

2. The herbal formulation for the prevention and treatment of bee brood as claimed in claim 1, wherein: the rhizoma corydalis, the barbed skullcap herb, the sweet wormwood herb, the honeysuckle flower, the amur corktree bark, the astragalus root, the desmodium and the liquorice are crushed, mixed and soaked in the honey to be prepared into paste.

3. A preparation method of a Chinese herbal medicine preparation for preventing and treating bee rotten son disease is characterized by comprising the following steps:

4. The method for preparing a Chinese herbal medicine preparation for preventing and treating bee rotten son disease as claimed in claim 3, wherein: the rhizoma corydalis, the barbed skullcap herb, the sweet wormwood herb, the honeysuckle flower, the amur corktree bark, the astragalus root, the desmodium and the liquorice are crushed into 100 meshes.

5. A Chinese herbal medicine preparation for preventing and treating bee rotten son disease is characterized in that the application method of the Chinese herbal medicine preparation comprises the following steps: the Chinese herbal medicine preparation for treating bee rotten son disease of the claim 1 or 2 is put on the upper beam of a hive frame of a bee colony, the amount of the Chinese herbal medicine preparation corresponding to each frame of bees is 5 grams, and the Chinese herbal medicine preparation is fed for 1 time every other day and is continuously fed for 3-5 times.

6. A Chinese herbal medicine preparation for preventing and treating bee rotten son disease is characterized in that the application method of the Chinese herbal medicine preparation comprises the following steps: the Chinese herbal medicine preparation for bee brood according to claim 1 or 2 is put on the upper beam of the hive frame of a bee colony, the amount of the Chinese herbal medicine preparation corresponding to each frame bee is 5g, and the Chinese herbal medicine preparation is used for 1 time in one month.