KR100806294B1 - Tradional oriental additive using eel - Google Patents

Abstract

A herb medicine additive used in the feed for cultivating an eel is provided to improve the preference to an eel and to enhance the growth and immunity of the eel with cultivated with the additive. A herb medicine additive comprise 10-12 wt% of Cnidium officinale; 15-18 wt% of Hordeum vulgare var.; 15-18 wt% of Massa medicata fermentata; 5-8 wt% of Atractylodis Rhizoma alba; 20-25 wt% of Atractyodis Rhizoma; 10-15 wt% of licorice root; and 15-20 wt% of Acanthopanacis Cortex.

Description

Herbal additives for eel {TRADIONAL ORIENTAL ADDITIVE USING EEL}

The present invention relates to an herbal additive for eel, and more specifically to digestion by adding a herbal additive consisting of seven herbal medicines, including cheongung, malt, new song, baekchul, creation, licorice, and ogapi to the nutrients contained in the feed. And it relates to an herbal additive for eel that can improve the productivity of eel farming by promoting absorption.

In recent years, the production of fish farming has increased due to the rapid development of breeding technology, but the deterioration of the breeding environment and the increase of stress have led to the deterioration of the activity of the fish, deterioration of growth, deterioration of meat quality and antibiotics. Facing misuse and abuse of drugs.

In particular, seafood products such as eel, which is a representative freshwater fish species, are increasing qualitatively and quantitatively every day due to an increase in national income and interest in health.

There are 16 kinds of eel and three subspecies of eel, and 19 kinds of eel are widely distributed all over the world. There are two kinds of eel and eel in Korea, but one species of eel is one species.

The eel is a descending fish, and the eels that have come up to the river grow into 300 ~ 1,000g for 4 ~ 12 years and become a sea urchin.It is a habitful species that spawns 7 ~ 12 million in early spring and summer. Since 1960, the government has been promoting the eel farming industry.

Although eel farming accounts for a high proportion of inland water production as the export volume of eel has increased, the development of eel-specific farm feed additives has not been studied at all. In view of this, there is a need for developing an eel cultured feed that can prevent disease while having better feed efficiency than a high-performance herbal additive or imported feed.

Recently, however, the national diet has been threatened by malachite green waves of Chinese eel, and the aftermath of domestic eel farming industry and freshwater farming industry has been shaken.

Despite the increase in eel consumption, the development of eel-specific feed additives has not been studied at all.

In view of this point, there is a need for developing an eel-specific culture feed composition capable of preventing diseases while having better feed efficiency than high-performance feed additives or imported feeds.

Seven or more of the main components of the present invention, such as cheongung, malt, new song, baekryeok, creation, licorice, ogapi are not only widely known as herbal medicines, but also have fewer side effects such as toxicity.

The characteristics and specific actions of each of these herbs are as follows.

Cnidium (Cnidium officinale Makino) is a root of perennial herb that belongs to the mountain and genus.

Malt (麥芽) is a medicine that has an effect on promoting digestion, and is a medicine that improves growth by facilitating digestion and absorption.

Singok (新 穀) is an herbal medicine that is effective in nourishment, digestion, and loss of appetite in oriental medicine, and facilitates digestion and absorption.

Baekchul (白 朮) is a herbal medicine, lumps of roots of the herb to say, as a medicine, it is an medicinal herb that helps the taste buds and is effective in digestion.

Creation (蒼朮) in the herbal medicine, the roots of the roots of the lumps of herbs, as a medicinal herb, to aid the taste and digestive effect, and to increase the immunity by fungi.

Licorice (Glycyrrhiza gabra Linne), Manchurian licorice (Glycyrrhiza uralensis Fischer) of the legume (Leguminosae), and the roots and gourds of the same plants as it is or removed the bark, blending Chinese herbs The drug is well represented, detoxification, immune cell activation, anti-inflammatory, immune function has an action.

Ogapi (五加皮) is the root of the Acanthopanax gracilistylus W.W.SMITH of the Araliaceae family, and is generally called South Ogapi. In oriental medicine, it is well known for its energy-boosting effects, geunhap, and Kang Geun-gol.

Each of these feeds is composed of the herbal medicines that are commonly used, each of the herbal medicines has a unique pharmacological action and main efficacy.

In the cultured fish feed using the same, "Korean herbal additive composition for promoting growth of cultured fish" containing malt and new grain is disclosed in Korean Patent Publication No. 1998-100, and Korean Patent Publication No. 1999-0011558 include Baekchul and Cheongung. "Bangami flounder and flat flounder feed and a manufacturing method thereof" are disclosed, and Japanese Patent Laid-Open No. 2005-0123002 discloses "Fish for farming fish supplemented with thorns." However, these technologies are not focused on developing new growth-promoting feeds suitable for eel, and the development of aquaculture feeds has been carried out through long-term breeding experiments to accurately identify the nutrient types and needs of each fish species. Since the selection of economical raw materials is required, the composition of aquaculture feeds for eel from feed compositions limited to conventional cicada and flounder could never be easily derived without accurate experimental results. Add medicinal herbs such as soybean paste, jujube, licorice, and Angelica, and use the functional herbal medicine that limits the content of thorny opi extract in fish feed for fish farming. There was a problem that could not be.

Therefore, the present inventors selected 44 candidate herbal medicines based on the principle of herbal medicine to maximize the effect on the eel and maximize the action and the characteristics of each of the herbal medicines from the herbal additives based on this The present invention was completed by finding a combination of herbal materials having remarkable effects through an experimental experiment on herbal aquaculture feed.

Therefore, the present invention has been made in order to solve the above problems, and after selecting a particular herbal medicine having a particularly antibacterial activity against diseases occurring in farmed eel among a number of herbal medicines, the eel characterized in that it contains a predetermined amount of the herbal medicine The purpose is to provide a Chinese herbal additive.

In addition, the present invention is another object to provide an herbal additives for eel that can maximize the profitability of aquaculture producers by exhibiting excellent efficacy and growth rate compared to the general feed feed.

Herbal additives for eel according to a preferred embodiment of the present invention in order to achieve the above object, 10 to 12% by weight, malt 15 to 18% by weight, 15 to 18% by weight, 5 to 8% by weight, It is characterized by consisting of 20 to 25% by weight, 10 to 15% by weight licorice, 15 to 20% by weight.

Hereinafter, the configuration of the present invention will be described in detail.

Herbal additives for eel according to the present invention is characterized in that the herbal additives are added to general aquaculture feeds such as fish meal, starch, vitamins, minerals, etc. The present invention is antimicrobial to the eel from the natural herbal medicines that can be obtained in Korea This can be done at the stage of selecting a specific natural herbal medicine with excellent history and growth potential.

1. Extraction of active ingredients of herbal medicines

1) Selection of herbal medicines

In the present invention, the antimicrobial measurement test for fish proceeds from the step of selecting 44 kinds of herbal medicines through domestic and foreign literature.

In the present invention, the 44 kinds of herbal medicines can be obtained in Korea as a herbal medicine in each herbal medicine by selecting a specific site such as root, stem, branch, fruit, etc. Extract 44 kinds of herbal medicines selected as natural herbal medicines are shown in Table 1 below.

Name of herbal medicine Used area Korean name Scholarship Licorice Glycyrrhiza uralensis Root Lacquer Rhus trichocarpa skin Wolfberry Lycium chinense Fruit Blood clots Mucunae Caulis Branch Party ear Angelica gigasNakai Root Only three Codonopsis pilosula Root Rhubarb Elsenia bicyclis Root Two tooth Eucommiae ulmoides leaf Macmundong Liriopis tuber Root Malt Peppermint Mentha arvensis leaf Earl Paeonia japonica Root Backout Atractylodes japonica Root Wealthy Aconitum carmichaeli Root Casualties Torilis japonica seed Cornus Cornus officinalis Fruit Three hundred seconds Saururus chinensis leaf Trilobite vinegar Epimedium koreanum leaf White skin Mori cotex radicis Root Heartache Morus alba Fruit Sun hat Glandular hair Leaf, stem Chain sheep Cynomorium songaricum leaf Hungjiwang Rehmannia glutinosa Root new song Lobe Artemisiae asiaticae Leaf, stem Journey Room Ligustrum lucidum Fruit Bridge Forsythia koreana seed Territory Ganoderma lucidum Leaf, stem Ogapi canthopanax gracilistylus W.W.SMITH stem Schisandra Schizandra chinensisis seed Broiler Cinnamomum cassia skin Breeding Boschniakia rossica Leaf, stem Yulmu Coix lachrymajobi Root Ginseng Panax ginseng Root Yu Yu Sanguisoba officinalis Root, stem Creation Atractycodis Rhizoma Root Astronomical building Asparagus cochinchinensis Root Tosa Cuscuta japonica seed Pagokcheon Morindae radix Root Shouao Polygonum multiflorumthunberg Root Nasturtium Eclipta prostrata Leaf, stem Hyun Sam Scrophularia ningpoensis Root Yellow ear Astragalus membranaceus Fruit Gold Scutellaria baicalensis Root

2) Preparation of herbal medicine extract

The present invention proceeds by a method of measuring the antimicrobial activity of each of the herbal medicines by the hydrothermal extraction and alcohol extraction, and then concentrated and lyophilized each of the 44 herbal medicines.

Hot water extraction method is a method of adding 50 g of each herbal medicine and 1500 ml of distilled water described in Table 1 in a water bath (Daewoong electric DWT 2800T ^TM ), and then continue for 3 hours, alcohol extraction method is shown in Table 1 40 g of each herbal medicine described and 800 ml of ethyl alcohol are added, followed by shaking for 24 hours in a shaking incubator. After hot water extraction and alcohol extraction in the same manner as described above, the measurement results of converting the yield (g) of each medicinal herb to the gallnut dry weight 100g are shown in Table 2 below.

Herbal Medicine Hydrothermal extraction Alcohol extraction Herbal Medicine Hydrothermal extraction Alcohol extraction Licorice 32.71 28.72 Sun hat 15.97 11.49 Lacquer 9.61 7.28 Chain sheep 22.95 22.65 Wolfberry 44.37 31.07 Hungjiwang 66.07 37.62 Blood clots 20.6 16.90 new song 35.26 45.39 Party ear 44.55 43.40 Lobe 15.60 12.35 Only three 46.93 20.67 Journey Room 19.53 15.44 Rhubarb 31.90 27.70 Bridge 12.48 10.75 Two of 73.09 23.49 Territory 6.80 4.87 Macmundong 73.09 23.49 Schisandra 38.87 41.48 Malt 79.26 53.01 Broiler 8.23 6.59 Peppermint 29.42 18.83 Breeding 49.69 49.74 Earl 27.07 20.76 Yulmu 11.33 4.94 Backout 72.08 23.42 Ginseng 36.93 15.83 Wealthy 19.86 4.66 Astronomical building 38.82 52.36 Casualties 12.42 5.17 Tosa 62.77 5.85 Cornus 32.01 2.36 Pagokcheon 57.73 38.06 Three hundred seconds 23.35 12.06 Shouao 24.01 23.85 Trilobite vinegar 20.34 13.84 Nasturtium 12.78 8.98 White skin 10.67 5.46 Hyun Sam 53.44 47.03 Heartache 10.48 8.32 Yellow ear 28.85 8.08 Ogapi 85.36 45.96 Gold 56.34 24.13 Yu Yu 32.36 63.25 Creation 86.34 23.46

3) Concentration and lyophilization of herbal extracts

The hot water extraction and the alcohol extracted extract were vacuum-filtered, and the extract component was concentrated under reduced pressure in a 60 ° C. hot water bath using a rotary vacuum evaporator (Buchi RE 121 ^™ , Switzerland), and concentrated as much as possible. Thereafter, lyophilized with a freeze dryer (FD EYELA ^TM ) to powderize, the powdered dry sample is stored in a deep freezer of -78 ℃, measuring the dry weight of each dry sample to use in each experiment desirable.

2. Antimicrobial Activity of Herbal Medicines

1) Selection of Fish Bacteria for Antimicrobial Activity Test

Experimental strains used in the antimicrobial assays of the present invention are the major causes of mortality in freshwater eel culture, Aeromonas hydrophila, Flexibacter columnaris, Edwardsiella tarda and Pseudomonas. strains of anguilliseptica) are used.

2) Preparation of Paper Disk for Antimicrobial Activity Test

The lyophilized powder by hot water extraction was dissolved in sterile distilled water, and the lyophilized powder by alcohol extraction was dissolved in ethyl alcohol.

First, 0.1 g of dry powder is dissolved in 2 ml of sterile distilled water in the case of lyophilized powder by hot water extraction, and 0.1 g of dry powder is dissolved (50,000 ppm) in 2 ml of ethyl alcohol in the case of alcohol extraction. Each of the dissolved solutions was filtered with a thin film filter (0.45 μm), and then it was absorbed by 50 μl in paper discs (ADVANTEC D0811606 ^™ , 8 mm), respectively. Thereafter, immediately placed in a drier adjusted to 50 ° C and sufficiently dried, it is used for antimicrobial experiments. At this time, the drug content of the paper disk is 2,500 µg.

3) Antimicrobial activity test for fish disease bacteria

The strains used for the antimicrobial activity test were four selected strains of Aeromonas hydrophila, Bacterial gill disease (Flexibacter columnaris), Edwardsiella tarda, and Pseudomonas anguilliseptica.

Strain bacteria for the antimicrobial activity test MacFaland No. After adjusting to 0.5, evenly spread 200 μl of the bacterial solution on Muller Hinton plate medium and incubate at 25 ° C. for about 48 hours. Thereafter, each herbal medicine paper disc prepared above is placed and incubated in a 25 ° C. incubator for 24 hours, and then lowlands are measured with a vernier caliper.

As a result of measuring the antibacterial activity of each herbal medicine by the above method, the hot water extract was as shown in Table 3 below, and in the case of alcohol extracts as shown in Table 4 below.

Herbal Medicine Aeromonas hydrophila Flexibacter columnaris Edwardsiella tarda Pseudomonas anguilliseptica Licorice - - - - Lacquer - ++ ++ +++ Wolfberry - - - - Blood clots - + + - Party ear - - - - Only three - - + - Rhubarb - - - - Two tooth - - - - Macmundong - - - - Malt + +++ ++ ++ Peppermint - - - - Earl - + - - Backout ++ +++ - ++ Wealthy - - - - Casualties - - - - Cornus - - - - Three hundred seconds - - - - Trilobite vinegar - - - + White skin - + + + Heartache - - - - Sun hat - + + - Chain sheep - - - - Hungjiwang - - - - new song ++ - - ++ Lobe + ++ - - Journey Room - - - - Bridge + + - - Territory - - - - Ogapi ++ ++ + + Schisandra - + + - Broiler - + + + Breeding - - - - Yulmu - + - - Ginseng + - + - Yu Yu + + - - Creation ++ +++ + ++ Celestial palace ++ + ++ + Astronomical building - - - - Tosa - - - - Pagokcheon - - - - Shouao - - - - Nasturtium - - - - Hyun Sam - - - - Yellow ear - - - - Gold + + + -

Herbal Medicine Aeromonas hydrophila Flexibacter columnaris Edwardsiella tarda Pseudomonas anguilliseptica Licorice - - - - Lacquer ++ +++ - Wolfberry - - - - Blood clots - + + - Party ear - - - - Only three - - + - Rhubarb - - - - Two tooth - - - - Macmundong - - - - Malt + ++ + + Peppermint - - - - Earl - - - - Backout ++ ++ ++ + Wealthy - - - - Casualties - - - - Cornus - - - - Three hundred seconds - - - - Trilobite vinegar - - - + White skin - + - - Heartache - - - - Sun hat - - - - Chain sheep - - - - Hungjiwang - - - - new song +++ + ++ + Lobe + ++ - - Journey Room - - - - Bridge + + - - Territory - - - - Ogapi ++ +++ + + Schisandra - + + - Broiler - - + + Breeding - - - - Yulmu - - - - Ginseng - - + - Yu Yu + ++ - ++ Creation + ++ + + Celestial palace +++ + ++ + Astronomical building - - - - Tosa - - - - Pagokcheon - - - - Shouao - - - - Nasturtium - - - - Hyun Sam - - - - Yellow ear - - - - Gold + - + -

(However, in the above table, + and-are the diameters of the clean zone, which are respectively-, 8 mm or less; +, 8-15 mm; ++, 15-20 mm; +++, 20 mm or more)

3. Specific of herbal medicine

1) Analysis of Experiment Results

According to the results of the experiment, the hot water extract showed the highest antimicrobial activity of the rake, malt, whitening and creation, and the alcohol extract, the new song, Ogapi, fat milk and cheongung extract was determined to have a relatively good antibacterial activity. However, in the above case, the lacquer bark had very low antibacterial activity against the Aeromonas hydrophila strain, and the fat milk had a disadvantage in that it could hardly exhibit the antibacterial activity against the Edwardsiella tarda strain.

On the contrary, the cheongung, malt, singok, baekryeok, creation and organ extracts were confirmed to have excellent antimicrobial activity in both hot water extracts and alcohol extracts.

2) Identification of Herbal Herbs for Eel Feed

Therefore, the present inventors have concluded that it is most preferable to use cheongung, malt, new song, baekchul, creation, and agapi as a natural antimicrobial agent for fish bottles of eel. By adding licorice to neutralize the drug, it was decided to add the herbal additives to the eel feed.

Looking at the ingredients added as an herbal additive, as a herbal medicine, Cheonggung, malt, new song, baekchul, white licorice, licorice, ogapi ) Is used.

Hereinafter, to describe the pharmacological action of the herbal medicine described above.

-The arch

Cnidium (川芎, Cnidium officinale Makino) is a root of a perennial plant belonging to the mountain-shaped and Cnidium, Cnidium (Cnidium officinale MAKINO), Chinese Ligusticum chuanxiong HORT, Ligusticum wallichii FRANCH var. officinale YOOK, Ligusticum monnieri ), Ligusticum japonicum and Ligusticum lucidum roots. The cheongung is a medicinal herb that improves the feeding power of the eel with a strong scent as a medicinal herb that has the effect of releasing neovascularization and congestion. Therefore, it is preferable that the content of cheongung in the total herbal additive composition is 10 to 12% by weight. If the content is less than 10% by weight, the effect of addition is insignificant. have.

-malt-

Malt is important as a raw material for beer brewing, and is also widely used for preparing microorganism incubators. Sugars such as starch (비타민), vitamins are contained, and the amylase is strong to improve the digestive function of the eel and to turn the blood well. Therefore, the content of malt in the whole herbal additive composition is preferably 15 to 18% by weight. If the content is less than 15% by weight, the effect of addition is insignificant, and when the content exceeds 18% by weight, there is a problem of excessive digestion function. .

-new song-

Singok (新 穀) is a medicine that is effective for nourishment, digestion, and loss of appetite, and facilitates digestion and absorption to improve eel growth. Therefore, it is preferable that the content of baekchul in the total herbal additive composition is 15 to 18% by weight. If the content is less than 15% by weight, the effect of addition is insignificant, and if it exceeds 18% by weight, there may be a problem in palatability.

Creation, Whiteness

Meanwhile, Atractylodes lancea De Candolle is a perennial plant belonging to the Asteraceae. The stem is relatively hard, and the upper part of the stem is branched and the height is about 35cm to 80cm. This difference in height depends on the conditions of the land of the shovel. Leaves are alternate, petioles are long and hard, usually split into idols, oval ellipsoid, with rough and pointed teeth. Fruits are aquatic and young leaves are fragrant wild vegetables and have been common sense in Korea for a long time. Root stock is called Atractycodis Rhizoma and is used for medicinal purposes.

The essential ingredient contains more than 1.5% of essential oils, and the main components are atractylodin, atractylodin, vitamins A and D, and the like. Creation is favored as diuretic (利尿) and old wind medicine (軀 風 藥).

In Korea, the roots of the same plant are well developed and enlarged, picked and peeled and medicated, which is called baekchul (白 朮). Baekchul contains 1.4% of essential oils and its main components include atractylol, atractylone, vitamin A and the like. However, atractylone is resinized in air to produce two crystals of eudesmol and hynesol by oxidation, and when contacted and reduced, tetrahydroalantolactone Generate a crystal. This crystal has been shown to have a side effect of reducing the body's water content, which has been shown to interfere with growth. Therefore, the content of Baekchul in the total herbal additive composition is preferably 5 to 8% by weight. If the content is less than 5% by weight, the effect of addition is insignificant, and if it exceeds 8% by weight, there is a problem of the above side effects.

However, unlike the main component of the production, the creation is atlactylodin (atractylodin), and there is little or no atactic lactone (atractylone) that is the main component of the extraction. Therefore, creation is made by supplying blood and related to the stomach, so it helps the eel's taste buds and is effective in digestion, and improves immunity by fungi. In addition, the content of the creation of the total herbal additive composition is preferably 20 to 25% by weight, the content is less than 20% by weight, the effect is negligible, if it exceeds 25% by weight may have a problem in palatability.

-licorice-

The licorice (Glycyrrhiza uralensis Fischer et.De Candolle) (Manchurian Mongolia) uses a perennial herb, a perennial herb, belonging to the legume family. Licorice grows from spring roots, grows to about 1m, and has hairs attached to the entire stem. The leaves are regenerated, and the pterygium is formed in the shape of a round shape, and in early autumn, light red leaves are formed, and after flowering, conical fruits are deleted. Usually the length is about 1m, the thickness reaches 2cm and generally does not branch. The outer surface is reddish brown. The main ingredient contains sweetener, Clycyrrhizin (C ₄₂ H ₆₆ O ₁₈ ), and other linquiritin (C ₂₁ H ₂₂ O ₉ ), sucrose, glucose, mannite, asparagine ( Asparagin), resin, etc., is non-toxic and tastes sweet. Licorice neutralizes medicine and is effective in improving eel by detoxifying habaekbaek (解 百 藥 毒). The content of licorice in the total herbal additive composition is preferably 10 to 15% by weight. If the content is less than 10% by weight, the effect of addition is insignificant, and if it exceeds 15% by weight, there may be a problem of hypokalemia. have.

Ogapi

Ogapi is composed of seven types of triterpenoid glycosides (Ereuterosides A, B, C, D, E, F, G) as main components, and other sugars such as glucose and galactose. And abundant amounts of carotene, vitamins B1, B2, C, and minerals, and glycosides such as sterols, coumarins, ligunin, flavones, and futalein. In particular, coumarin has a remarkable calming effect, which helps the eel in the laying season to maintain a stable state, and flavones expand the coronary artery and increase the amount of blood to prevent eel oxygen deficiency.

In addition, Ogapi contains a component that inhibits the production of lipid peroxide. One of these is tannins, which are called `` Gloroic Acid '' and `` Gigapeoirkilic Acid ''. Glogenic Acid inhibits the rise of serum triglycerides and lipid peroxides, improves lipid metabolism, and secretes gastric juice. It has a promoting effect and has a powerful antioxidant. In particular, the antimicrobial component of the organo is one of the main components, ereuteroside E, which is responsible for many physiological activities such as antitumor, antiallergic as well as antibacterial activity. Therefore, Ogapi was found to be highly resistant by enhancing the eel's motility, strengthening the conditional reflex action, and reducing the basic metabolism and blood sugar levels to enhance immunity to the disease. Clinical trials have a radioprotective effect, inhibiting tumor cell adhesion and metastasis to other tissue cells, and its effects and efficacy have been scientifically proved, making it highly applicable as a natural substance. It is preferable that the content of Ogapi in the whole herbal additive composition is 15 to 20% by weight. If the content is less than 15% by weight, the effect of addition is insignificant, and if it exceeds 20% by weight, there may be a problem in palatability.

The preparation method of the herbal additives according to the present invention is 10 to 12% by weight, malt 15 to 18% by weight, 15 to 18% by weight of new songs, 5 to 8% by weight, 20 to 25% by weight, licorice 10 to 15% by weight. %, Ogapi 15 to 20% by weight to mix in proportion of each weight percent. The preparation of the herbal additives may be mixed after pulverizing each raw material as described above, but may also be subjected to a process of mixing each raw material which has been carried out before the grinding process, and then crushing them together in a batch.

Then, the herbal additives are added to commercially available fish meal, starch, vitamins, minerals, etc. in the range of 0.5 to 5.0 parts by weight based on 100 parts by weight of feed. In the application of the herbal additives of the present invention to the feed content of about 0.5 to 5.0 parts by weight based on 100 parts by weight of the feed composition is sufficient, if the addition amount of the herbal additives is too small compared to the general feed it serves as a herbal additives It is difficult to do so, and when the amount is excessive, the eel cannot maintain sufficient nutrition, and there may be a problem in economy and palatability.

At this time, the feed composition feed amount of the eel is most preferably 20g ~ 30g, when the eel is less than 20g intake of the feed composition is very poor nutrition.

In addition, the feed composition of the feed to the eel is preferably divided into several times at regular intervals during the day, it is usually divided into 2 to 4 times in management.

3) Experimental results by mixing conditions of herbal medicines

In the preparation of 1kg of a typical eel herbal additive according to the present invention, it was tested as shown in Table 5 to select the appropriate blending conditions for cheongung, malt, new song, white rice, produce, licorice, scabies raw material.

Example 1 Preparation of Herbal Additives for Eels

The herbal medicine powder having a particle size of 60 to 100 mesh was prepared by washing and drying the selected Cheongung, malt, new song, white rice, produce, licorice, and organza according to the ingredient ratios of Table 5 below.

10 kg of herbal additives prepared as described above and 1,000kg of conventional eel cultured feed consisting of fishmeal, starch, vitamins, minerals, etc. were prepared in the form of powder, to prepare the eel herbal dietary feed of the present invention.

Herbal Medicine Contents Table (Unit Weight%) to Determine Proper Blend Ratio Compound treatment Cheongung malt new song Whiteness Creation licorice Ogapi Treatment Zone 1 10 25 25 5 5 15 15 Treatment 2 10 10 10 10 20 20 20 Treatment Zone 3 15 15 15 15 30 5 5 Treatment Zone 4 10 15 15 5 20 10 20 Treatment Zone 5 12 18 18 5 22 10 15 Treatment 6 10 18 15 5 15 12 25

As shown in Table 5, the survival test results of the eel feeding the herbal culture feed composition for each formulation are shown in Table 6 below.

The tank for eel farming is a horizontal × vertical × height = 1.8m × 1.8m × 1m sized aquarium in a greenhouse equipped with a shade screen to maintain automatic temperature (28 ~ 29 ℃) control, oxygen supply, direct sunlight shading, and quietness. The dogs were installed on the same scale.

The water level of the culture tank was set to about 50cm, and a circulation pump using a drop method was installed to supply oxygen smoothly, and 36,400 rice eel of 4.4g in each culture tank was started.

According to the presence or absence of the addition of the herbal additives for eel prepared in Example 1 was classified as follows.

* Control group: Group administered only commercial eel farmed feed containing no herbal additives for eel according to the present invention .

* Herbal experimental group (mixture treatment 1 ~ 6): the group added the herbal additives for eel according to the present invention to the control feed .

The feeder was placed just below the water surface with a basket of the appropriate size attached to the wall of each culture tank. As shown in Table 5, after adding the ingredients of each formulation to the herbal test group feed, it was made into a dough form using a feed maker to supply the amount to eat all within one hour within 3% of the weight of the eel per day. Sixty days of comparative breeding with experimental diets were performed to count the total number of eel surviving in each tank and the number of eel that died during the comparative breeding period.

Hereinafter, the survival test results of the eel according to the comparative breeding are shown in Table 6 below.

Survival rate division Control Herbal Experiment Group Treatment tool 1 Treatment 2 Processing Unit 3 Processing Unit 4 Treatment Tool 5 Treatment Tool 6 Total breeding number (US) 36,400 36,400 36,400 36,400 36,400 36,400 36,400 Total Number of Deaths (US) 230 120 85 136 5 12 64 Final survival attempt (US) 36,170 36,280 36,315 36,264 36,395 36,388 36,336 Survival rate 99.36% 99.67% 99.76 99.62% 99.99% 99.99% 99.82%

As can be seen from Table 6, the survival rate of the eel showed a 99.99% survival rate in the treatment groups 4 and 5 of the herbal experiment group compared to the control group (99.36%) only 60 days after the experimental feed tour herbal medicine group compared to the control group In the treatment groups 4 and 5 of the mortality was shown that there is almost no improvement in the immune power of the eel and the farm environment.

Thus, 10% by weight of the hemunggi of treatment 4, 15% by weight of malt, 15% by weight of new grains, 5% by weight of oak, 20% by weight, licorice 10% by weight and 20% by weight of scabies and 12% by weight of uterus of treatment 5, 18% by weight of malt, 18% by weight of new song, 5% by weight of white rice, 22% by weight of produce, 10% by weight of licorice and 15% by weight of ogarium were found to be the most dead.

< Experimental Example  2: weight measurement experiment according to dietary intake>

The present inventors prepared herbal additives based on the results obtained in Example 1 and Table 6. That is, the herbal additives 10 to 12% by weight, malt 15 to 18% by weight, 15-15% by weight of new songs, 5 to 8% by weight, creation 20 to 25% by weight, licorice 10 to 15% by weight, Ogapi 15 ~ 20 weight percent.

In addition, the measurement results of the weight gain of the surviving eel of Table 6 are shown in Table 7 below.

Results of weight measurement according to dietary intake division Control Herbal Experiment Group Treatment Zone 4 Treatment Zone 5 Total breeding number (US) 36,400 36,400 36,400 U.S. sugar starting weight (g) 4.4 4.4 4.4 Total Starting Weight (kg) 160 160 160 Total feed grade (kg) 1,039 1,039 1,039 End of body weight (g) 23.8 29.7 28.3 Total ending weight (kg) 861 1,081 1,072 Total weight gain (kg) 701 921 896 Feed efficiency (%) 69.4 88.6 86.2 Feed factor 1.44 1.13 1.18

As can be seen from Table 7, the weight of the eel was the largest in weight gain (growth) in the treatment group 4 (29.7g) of the herbal test group compared to the control (23.8g) after 60 days of the experimental feed tour.

In addition, feed efficiency increased by 19.2% in treatment group 4 (88.6%) and 16.8% in treatment group 5 (86.2%) compared to control group (69.4%). appear.

< Experimental Example  3: Immunity and Anemia Related Index Measurement Experiments>

Hereinafter, the experimental results of the index of measurement of immunity and anemia of the eel according to the comparative breeding are shown in Table 8 below.

Immunity and Anemia Index Test Results division Control Herbal Experiment Group Treatment Zone 4 Treatment Zone 5 White blood cell (WBC) number (10 * 3 / μL) 153.26 ± 2.06 ab 166.32 ± 3.21 ab 162.14 ± 2.81 ab Lymphocyte count (%) 38.98 ± 4.34 a 48.69 ± 6.12 a 46.45 ± 5.43 a RBC count (10 × 6 / μL) 2.34 ± 0.02 a 2.46 ± 0.13 a 2.42 ± 0.08 a Platelets (10 × 3 / μL) 12.67 ± 1.35 a 13.95 ± 2.56 a 13.74 ± 1.86 a

Each value is the mean ± standard deviation of 10 US per group.

For each group, the experimental results were analyzed by one-way analysis of variance. The significance test between the means was statistically processed at the level of p <0.05 by Duncan's multiple range test.

As can be seen from Table 8, the number of white blood cells (WBC) can be seen in the statistically significant trend of increase in the treatment group 4 and 5 of the experimental group compared to the control group.

In addition, the lymphocyte number (Lymphocyte) number showed a significant increase of 24.9% and 19.2% compared to the control group, it can be seen that the natural immunity enhancer action by the herbal additives.

In addition, the essential red blood cell (RBC) count and platelet test associated with anemia were statistically significant increase of 5.1%, 10.1%, and 3.4% and 8.4%, respectively, in the herbal treatment group 4 compared to the control group. It shows a tendency to show an anemia prevention effect.

Therefore, it can be seen that the culture feed for eel containing the herbal additives has improved the natural immune system.

That is, in the above breeding experiments, an improvement in the feed force, growth rate and immunity was recognized for the eel herbal additives containing herbal medicines of cheonggung, malt, new song, baekchul, creation, licorice, and ogapi.

It is considered that this is because the digestive absorption rate of the feed is improved by adding the herbal additive according to the present invention.

Therefore, from the above, the herbal additive for eel according to the present invention promotes digestion and absorption after feed intake, alleviates contamination of breeding water by those excreted by eel, and promotes a healthy environment for raising healthy eel. It is to make it.

According to the present invention, when a continuous amount of eel is added to the eel continuously, a certain amount of a special compounding ratio of the seven herbal medicines including cheonggung, malt, new song, white peach, creation, licorice, and ogapi as an active ingredient The eel of the sphere can be seen that the immunity is greatly increased compared to the eel of the control. Therefore, when the herbal additive for eel according to the present invention is fed to the eel, it is possible to sufficiently secure the immunity against fish disease without administering the antibiotics currently used, thereby effectively preventing the infection of the eel by bacteria. It has the effect of replacing antibiotics.

In addition, when the herbal additive for eel according to the present invention is administered to the eel, by using a natural herbal medicine as an antibiotic, it is possible to supply a safe and healthy eel without using antibiotics, thereby reducing the side effects of the residual antibiotics currently in question Since it can be solved fundamentally, there is an advantage that can contribute to the promotion of consumption of fish by bringing about the change of recognition as national favorite food.

In addition, when the herbal additive for eel according to the present invention is administered to the eel, the fat content, serum lysozyme, and macrophage free radicals are greater than that of the general eel over time, and thus, the effect of preventing the disease is excellent and the natural immune system is enhanced. There is an effect of improving fish disease resistance.

In addition, when the herbal additives for eel according to the present invention are administered to the eel, the productivity and taste are improved by feeding force and growth promoting action, compared to the general feed feed, it is effective to maximize the profitability of aquaculture producers.

Claims (1)

10 to 12% by weight, 15 to 18% by weight of malt, 15 to 18% by weight of new song, 5 to 8% by weight of white rice, 20 to 25% by weight, 10 to 15% by weight of licorice, 15 to 20% by weight of scallop Herbal additives for eel, characterized in that.