KR101413283B1 - The method for manufacturing panax ginseng composite by using the complex extract for the enhancement of immunity, and the panax ginseng composite made by the method - Google Patents

Abstract

The present invention relates to a method for manufacturing a ginseng composite and a ginseng composite by using a complex for immunity enhancement. The immunity enhancement complex uses peony, cinnamomum cassia shoot, licorice root, ginger, jujube, and black taffy. The present invention relates to a method for manufacturing a ginseng composite including the steps of generating an immunity enhancement complex by mixing and extracting 60-90 parts by weight of peony, 50-80 parts by weight of cinnamomum cassia shoot, 10-30 parts by weight of licorice root, 40-70 parts by weight of ginger, and 40-70 parts by weight of jujube based on the 100 parts by weight of black taffy; and mixing 80-120 parts by weight of a ginseng extract based on 100 parts by weight by the immunity enhancement complex. The present invention relates to the method for manufacturing the ginseng composite including the steps of generating the immunity enhancement extract by mixing and extracting 60-90 parts by weight of peony, 50-80 parts by weight of cinnamomum cassia shoot, 10-30 parts by weight of licorice root, 40-70 parts by weight of ginger, and 40-70 parts by weight of jujube based on the 100 parts by weight of black taffy; manufacturing a ginseng inclusion compound by making a ginseng extract or ginseng powder and β-cyclodextrin react as 1:2.5-3; and mixing 80-120 parts by weight of the ginseng extract based on 100 parts by weight of the immunity enhancement complex. The present invention relates to the ginseng composite manufactured by the manufacturing method.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a ginseng composition and a method of manufacturing the ginseng composition using the immune enhancing complex for improving immunity,

The present invention relates to a method for manufacturing a ginseng composition and a ginseng composition using the immunostimulating complex for enhancing immunity, wherein the immune enhancing complex utilizes peony root, clam, licorice, ginger, jujube, and black peper.

The present invention is based on 100 parts by weight of black sugar, mixing 60 to 90 parts by weight of peanuts, 50 to 80 parts by weight of loam, 10 to 30 parts by weight of licorice, 40 to 70 parts by weight of ginger, and 40 to 70 parts by weight of jujube, Generating a complex; And mixing 80-120 parts by weight of the ginseng extract with respect to 100 parts by weight of the immunostimulating complex.

The present invention is based on 100 parts by weight of black sugar, mixing 60 to 90 parts by weight of peanuts, 50 to 80 parts by weight of loam, 10 to 30 parts by weight of licorice, 40 to 70 parts by weight of ginger, and 40 to 70 parts by weight of jujube, Generating a complex; Preparing a ginseng inclusion compound by reacting a ginseng concentrate or powder with? -Cyclodextrin in a ratio of 1: 2.5 to 3.5; And mixing 80-120 parts by weight of the ginseng extract with 100 parts by weight of the immunostimulating complex.

The present invention relates to a ginseng composition prepared using the above-described method.

Immune refers to a mechanism for physiologically recognizing and excluding exogenous and endogenous foreign substances in humans and animals and for maintaining homeostasis. Immunity can be categorized into two broad categories: innate immunity from birth, and acquired immunity acquired through adaptation to life and the like.

Congenital immunity is also called natural immunity. It responds nonspecifically to the antigen and has no special memory effect. Congenital immune systems include skin and mucous membranes that block the entry of antigens, acidic gastric acid, and complement in blood. Cells include macrophages, polymorphonuclear leukocytes, and K cells that can kill infected cells. In fact, most infections are defended by this congenital immune system.

Acquired immunity is also called acquired immunity. You can remember about the first attacked antigen and react specifically when you re-enter it, effectively removing the antigen. It plays a role in reinforcing innate immunity.

Acquired immunity includes T cells and B cells. In the epithelial cells of the thymus, a cell that differentiates into a lymphocyte by a specific internal environment and a thymus liquefying factor is called a T cell, and a cell that is differentiated from the thymus and differentiates into a bone marrow to a lymphocyte is called a B cell. The role of T cells is to induce antibody production to B cells and to directly attack antigens such as tumor cells. The role of B cells matures into antibody-producing cells following antigen stimulation and T cell-mediated stimulation, and IgM, IgG , IgA, and IgE antibodies.

 "Health" refers to a state in which there is no abnormality in the body, that is, a state without disease or injury. A high immunity does not usually lead to disease, and even if you get sick, your recovery is fast. It is obvious that maintaining this healthy state and lengthening the life span by getting older. Therefore, immunity is directly linked to human health and longevity. In order to maintain health and extend life span, immunity management is essential.

However, if you look at the lifestyle of modern people, stress, lack of exercise, irregular life rhythm, unbalanced eating habits, smoking and immunity are the factors that will decrease many. There are many pollution factors in the environment and the immunity is also deteriorated. Therefore, modern people should strive to increase immunity.

There is a way to boost the immune system, such as Chimodulin, Bronchocobsum, is taking immunosuppressive drugs. The immunomodulatory agent contains calf thymus or purified bacterial body. The mechanism of action of the immunostimulating agent containing the purified bacteria is to train the immune system by administering the weakened bacterium to the body, and the action mechanism of the immunosuppressive agent containing the calf's thymus gland replenishes the T cell by ingesting the calf's thymus gland . However, the immune enhancer has very little effect, and there are side effects such as decreased appetite.

On the other hand, Panax ginseng CA Meyer, which is a traditional medicinal herb, has been widely known in Korea, China, and Japan, and has been used as medicines in many countries for many thousands of years.

Saponin, which represents the main effect of ginseng, is a constant compound called glycoside, which is found in roots, stems and leaves of ginseng. Saponin is composed of more than 30 kinds of ginsenosides. Ginsenoside is effective for health promotion throughout human body including geriatric diseases, aging prevention, alcohol detoxification and liver protection, anti-inflammation, improvement of hematopoietic function, .

However, due to the bitter taste of ginseng, the preference of young consumers and foreigners is lowered, which is a stumbling block to the high added value of ginseng products and their entry into overseas markets.

Accordingly, Applicant has proposed a method of manufacturing an immune enhancing complex which enhances the proliferation rate of macrophages, increases the NO production rate, and supplies a large amount of polyphenols and flavonoids to the body, thereby enhancing the immunity without using side effects Respectively.

In addition, the present applicant has not conducted a method of reacting cyclodextrin with ginseng to generate an inclusion compound so as to maintain the efficacy while eliminating the bitter taste of ginseng, and further increasing the immunity by mixing the immunoconjugate complex and the inclusion compound Respectively.

In the prior art related to the composition for enhancing immunity and the manufacturing method thereof, Korean Patent Laid-Open Publication No. 10-20009-0126681 discloses a herbal medicine extract of red ginseng, acacia, and ginseng cultured muscle and a health supplement containing immunoglobulin .

The present invention relates to a composition containing as an active ingredient a concentrated liquid obtained by extracting red ginseng, oogi, ginseng cultivating muscle, broiler chick, Angelica gigas, Astragalus membranaceus,

It is an object of the present invention to provide an immune enhancing complex for enhancing immunity without adverse effects by utilizing a substance derived from a natural source, and a ginseng composition using the complex.

It is an object of the present invention to provide an immune enhancing complex for enhancing immunity by increasing the proliferation rate of macrophages in the body and a ginseng composition using the complex.

It is an object of the present invention to provide an immune enhancing complex for enhancing immunity by increasing the amount of NO produced in the body and a ginseng composition using the complex.

It is an object of the present invention to provide an immune enhancing complex which enhances immunity by supplying a large amount of polyphenols and flavonoids into the body and a ginseng composition using the complex.

It is an object of the present invention to provide a ginseng composition which generates a ginseng inclusion compound that maintains its efficacy while eliminating the bitter taste of ginseng and further enhances immunity by mixing the immunoconjugate complex and the inclusion compound.

In order to solve the above-described problems, the present invention provides a method for producing a black liquor, comprising 60 to 90 parts by weight of peanut, 50 to 80 parts by weight of loam, 10 to 30 parts by weight of licorice, 40 to 70 parts by weight of ginger, Mixing parts by weight to produce an immunostimulating complex; And mixing 80-120 parts by weight of the ginseng extract with respect to 100 parts by weight of the immunostimulating complex.

The mixture of 60 to 90 parts by weight of peony fungus, 50 to 80 parts by weight of loquat, 10 to 30 parts by weight of licorice, 40 to 70 parts by weight of ginger and 40 to 70 parts by weight of jujube are mixed and extracted based on 100 parts by weight of black sugar, ; Preparing a ginseng inclusion compound by reacting a ginseng concentrate or powder with? -Cyclodextrin in a ratio of 1: 2.5 to 3.5; And mixing 80-120 parts by weight of the ginseng extract with respect to 100 parts by weight of the immunostimulating complex.

The present invention also provides a ginseng composition prepared using the above method.

Also, it is intended to provide a health functional food in which a food acceptable additive is added to the ginseng composition prepared using the above method.

The health functional food using the composition according to the present invention may be provided in various forms such as a functional beverage, a health supplement, tea, confectionery, and the like.

In order to use the present invention as a pharmaceutical composition, it may be prepared by a known method in the pharmaceutical field, and may be mixed with the carrier itself, or a pharmaceutically acceptable carrier, excipient, diluent or the like to prepare a powder, granule, Or injections, and the like. They may also be administered orally or parenterally.

The effective dose of the composition according to the present invention can be appropriately selected depending on the degree of absorption of the active ingredient in the body, the rate of water activation and excretion, the age, sex and condition of the patient, severity of the disease to be treated,

The composition according to the invention can be used in feed for livestock and in each case can be prepared using additives or additives which are acceptable in livestock feed.

Formulations such as pills, granules, beverages, tablets, capsules and the like may be prepared using the composition according to the present invention, in which case additives may be added to prepare each formulation.

The immunoconjugate complex and ginseng composition according to the present invention have the effect of not causing side effects by utilizing a substance derived from a natural origin.

The immunoconjugate complex and ginseng composition according to the present invention have the effect of enhancing the immunity by increasing the proliferation rate of macrophages in the body.

The immunostimulating complex according to the present invention and the ginseng composition have the effect of enhancing the immunity by increasing the amount of NO produced in the body.

The immunostimulating complex and the ginseng composition according to the present invention have the effect of increasing the immunity by supplying large amounts of polyphenols and flavonoids into the body.

The ginseng composition according to the present invention has the effect of improving immunity and eliminating the bitter taste and improving the preference degree.

1 is a graph showing an absorbance standard curve at 540 nm prepared using NaNO ₃ .
FIG. 2 is a graph showing the results of measurement of the amount of NO produced when RAW 264.7 cells were treated with the immunostimulating complex. FIG.
FIG. 3 is a graph showing the results of measuring the amount of NO produced when ginseng extract was treated with RAW 264.7 cells.
FIG. 4 is a graph showing the results of measurement of NO production when RAW 264.7 cells were treated with a ginseng composition prepared by mixing an immunostimulating complex and a ginseng extract. FIG.
5 is a graph showing a standard curve of total polyphenol contents prepared using gallic acid.
6 is a graph showing a standard curve of the total flavonoid content prepared using quercetin.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the embodiments described in the present specification, the reference examples, and the drawings are merely the most preferred examples of the present invention, and not all of the technical ideas of the present invention are described. Therefore, It should be understood that various equivalents and modifications may be present.

The immunostimulating complex according to the present invention uses peanut, glue, licorice, ginger, jujube, and black sugar as main natural materials, and will be briefly described below.

Paeonia lactiflora belongs to the family Ranunculaceae and is distributed in Korea, Mongolia and East Siberia. It is 40 ~ 50 cm in height. Its roots are thick and fleshy. Its bottom is covered with scaly leaves. It is split into several branches, thin and long and pointed cylindrical shape thick and long. Leaves and stems have no hairs. Leaves are alternate phyllotaxis and alternate phyllotaxis, and the lower part is a compound leaf with three small leaves. The veins and petiole are red. The leaf on the upper part is a leaf or a leaf which is simple in shape and has three small leaves. The surface of the leaf is glossy, the back is light green and the edge is flat. Flowers bloom one at the end of main stem and bloom in June. There are several operations and three or four ovaries. When the fruit is ripened, the inside becomes red, less grown red seed and mature black seed appear. Roots are used for analgesia, gynecology, and women's diseases, and hypertension, encephalitis, leukemia, gout, and rheumatism.

The lock Belonging to the camphor family It is a young branch of the broiler tree ( Cinnamomum cassia ). The country of origin of the broiler tree is China, and it lives in China, Sri Lanka and Indochina, and it is cultivated in Jeju in Korea. It grows up to 8 m in height. The small branch is green and has no hairs. Leaves are alternate phyllotaxis or opposite phyllotaxis, about 10 cm long, ovate long oval. The front face of the leaf is dark green and glossy, and the back side is indigo or white, with fine hairs. The flowers bloom in June and bloom in light yellowish green. Fruits ripen black in December. The stomach opens the pores of the skin at the initial cold, sweating, and relieves pain in the shoulders and back, and pain in the limbs. It also promotes blood circulation and cures ovarian failure. Other pharmacological actions include antibacterial and diuretic.

Licorice ( Glycyrrhiza uralensis ) belongs to the soybean family, and is distributed in Russia's Siberia, Iran, Afghanistan, Pakistan and China, in Gansu Province, Shingang Province, and Mongolia. The height is 30 to 80 cm, and the base is ligneous and has many branches. Young stems have hairs. The roots extend deeper into the rectus, and the phallus extends sideways. Leaves are alternate phyllotaxis, and petiole has cilia. The flowers bloom in purple, white and yellow, and the diameter of the flower is 1.0 ~ 2.4 cm. Licorice crust is reddish brown or dark brown with vertical wrinkles and occasionally shrubs, buds and scales. The outer skin of the peeled licorice is pale yellow and fibrous.

Licorice harmonizes the toxicity of all medicines so that they are effective, improve blood circulation, and strengthen muscle and bone components. It is effective for hepatitis, urticaria, dermatitis, eczema, peptic ulcer, etc. Other pharmacological actions include Jinhae genome, muscle relaxation, diuretic, anti-inflammation, and detoxification.

The ginger ( Zingiber officinale ) belongs to the ginger family, and its origin is Southeast Asia. It is distributed in East Asia, India and Malaysia. In Korea, it is cultivated mainly in Jeonbuk and Chungnam. At the root of the ginger, the fake stem made of leaf sheath grows at each node and its height reaches 30 ~ 50 cm. Leaves are alternate phyllotaxis. It does not bloom in Korea, but blooms in August in the tropics. Rootstocks are used for edible and medicinal purposes. Ginger is effective against colds, fever, headache, vomiting, seawater and sputum caused by cold. It also works for abdominal pain caused by food poisoning, diarrhea and complications. Other pharmacological actions to promote gastric secretion and digestive power, cardiac excitement, promote blood circulation, and antibacterial.

Jujube is the fruit of the jujube ( Zizyphus jujuba ) belonging to the family Seagull. It is distributed in Korea, China, southern Europe and Japan. It grows up to 7 ~ 8m in height, and the bark is gray brown. The young branches come out from one place and have a little hair at the end of the branch. Leaves are alternate phyllotaxis and ovate. The veins are clear and there are sawtooths on the edges. Flowers bloom in light green and bloom in June-July. Petals are smaller than calyx pieces, each 5 pieces. The fruit is oval-shaped nucleus with 2 ~ 3cm in length, ripened in green or reddish brown in September-October. Normally called 'jujube'. Jujube is used for edible and medicinal purposes. Diuretic, tonic, and emollient.

Black sugar syrup is made of glutinous rice. After cooling, the mixture is mixed with malt powder. After a certain period of time, when the clear water and sediment are separated, the precipitate is separated to make amber light. It promotes energy, promotes blood circulation, and provides moisture to the lungs. It also has an effect on cough, skin disease, and hemoptysis.

Hereinafter, the cyclodextrin will be described as follows. Cyclodextrin is a cyclic oligosaccharide produced by enzymatic reaction with starch as a raw material. The inside of the cyclodextrin molecule is composed of hydrogen and oxygen, and the outside is composed of a hydroxyl group. It is a white crystal or crystalline powder with no odor and a slight sweet taste. It has heat resistance against various acids and alkali, and is also resistant to heating and humidity.

The greatest characteristic of cyclodextrin is its inclusion. The inclusion is a phenomenon in which, when two molecules are paired, a relatively larger molecule spreads in a tubular, layered or net shape, and a smaller molecule is stuck into the gap to join the two molecules. The molecular structure of cyclodextrin is ring-shaped so that it can cover other molecules. The inside of the cyclodextrin molecule is hydrophobic and consists of hydrogen and oxygen, and the outside is hydrophilic.

The types of cyclodextrins can be classified into three types, i.e.,? -Cyclodextrin,? -Cyclodextrin and? -Cyclodextrin depending on classification items such as molecular weight, degree of polymerization and diameter. All categories have the lowest α-cyclodextrin in cyclodextrin, and the highest in γ-cyclodextrin.

Specifically, the degree of polymerization of? -Cyclodextrin is 6 and the molecular weight is 973. The diameter of the intracellular cavity of α-cyclodextrin is 0.47-0.53 nm, the inner cavity height is 0.79 nm, and the internal cavity area is 17.4 nm. The degree of polymerization of? -cyclodextrin is 7, and the molecular weight is 1135. The intramolecular cavity of β-cyclodextrin has a diameter of 0.60 to 0.65 nm, an internal cavity height of 0.79 nm and an internal cavity area of 26.2 nm. The degree of polymerization of? -cyclodextrin is 8, and the molecular weight is 1297. The diameter of the intramolecular cavity of γ-cyclodextrin is 0.75 to 0.83 nm, the internal cavity height is 0.79 nm, and the internal cavity area is 42.7 nm.

Manufacturing example  1. Ginseng Inclusion compound  Preparation: Acetic acid dissolution and pH  control

1-1. Ginseng concentrate manufacturing process

The ginseng used in this experiment was purchased in the wholesale market of Geumsan ginseng in Chungnam Province for 4 years after production in September, 2011. The ginseng was selected, washed, extracted 3 times with 7 ~ 10 times of the weight of ginseng. Extracts of ginseng extracts were prepared at concentrations of 60 ~ 70 brix at 70 ~ 90 ℃ and stored in refrigerator.

1-2. Ginseng powder manufacturing process

Ginseng of the same source as that of Production Example 1-1 was used. After removing the water of ginseng and freeze-drying, it was finely pulverized at 90 to 110 μm to prepare ginseng inclusion compound of β-cyclodextrin and ginseng.

1-3. Acetic acid Ginseng inclusion compound  Produce

3-7 g of ginseng concentrate or powder and 10-20 g of β-cyclodextrin are dissolved in 300-700 ml of 0.1 N acetic acid. 1.0 N sodium hydroxide (NaOH) was gradually added dropwise so that the pH of the solution became 10.0-12.0, and 0.1N hydrochloric acid (HCl) was added dropwise to the solution so that the pH of the solution became 7.0-8.0. The solution was reacted by filtration and freezing Dried or spray-dried to prepare a ginseng inclusion compound.

Preferably, the weight ratio of the ginseng concentrate or powder to? -Cyclodextrin is about 1: 3.

Manufacturing example  2. Ginseng Inclusion compound  Manufacturing: Ethanol dissolution

2-1. Manufacture of ginseng concentrate

Was prepared in the same manner as in Production Example 1-1.

2-2. Manufacture of ginseng powder

Was prepared in the same manner as in Production Example 1-2.

2-3. Dissolved in ethanol Ginseng inclusion compound  Produce

3 ~ 7g of ginseng concentrate or powder was added to 300 ~ 700ml of 40 ~ 50% ethanol solution and dissolved by stirring, then 10 ~ 20g of? -Cyclodextrin was added. The mixture was heated to 70-90 ° C and allowed to react for 2-5 hours, then the heating was stopped and stirring was continued at room temperature for 24 hours. After stirring was stopped, the reaction solution was filtrated under reduced pressure and freeze-dried or spray-dried to prepare ginsenoside inclusion compound.

Preferably, the weight ratio of the ginseng concentrate or powder to? -Cyclodextrin is about 1: 3.

Manufacturing example  3. Ginseng Inclusion compound  Manufacturing: dissolving in distilled water

3-1. Ginseng concentrate manufacturing process

Was prepared in the same manner as in Production Example 1-1.

3-2. Ginseng powder manufacturing process

Was prepared in the same manner as in Production Example 1-2.

3-3. Dissolved in distilled water Ginseng inclusion compound  Produce

10 ~ 20 g of β-cyclodextrin and 3 ~ 7 g of ginseng concentrate or powder were added to 200 ~ 600 ml of distilled water and dissolved by stirring at 35 ~ 40 ℃, followed by lyophilization or spray drying to prepare gangrene inclusion compound.

Preferably, the weight ratio of the ginseng concentrate or powder to? -Cyclodextrin is about 1: 3.

Manufacturing example  4. Of the immune enhancing complex  Produce

(1) 60 to 90 parts by weight of peanut, 50 to 80 parts by weight of licorice, 10 to 30 parts by weight of licorice, 40 to 70 parts by weight of ginger and 40 to 70 parts by weight of jujube were prepared and washed, based on 100 parts by weight of blackcurrant. Preferably, the peony is designed to use a vinegar.

(2) The washed black syrup, peanut, ginger, licorice, ginger and jujube were put into a container and mixed, and 700 to 900 parts by weight of purified water was injected relative to 100 parts by weight of the mixture.

③ The hot water extract of black peach, peony, licorice, licorice, ginger and jujube was prepared by heating the mixture of black peper, peony root, licorice, licorice, ginger, jujube and purified water to 75 ~ 85 ℃ and repeating this three times .

④ An immune enhancing complex was prepared by concentrating hot water extracts of black sugar, peony, licorice, licorice, ginger and jujube prepared at 35 ~ 45 brix.

Manufacturing example  5. Preparation of ginseng composition: Mix of ginseng extract and immunostimulating complex

5-1. Manufacture of ginseng extract

① 100 ~ 120 g of ginseng was selected and washed.

② The washed ginseng was put into a container and 8 ~ 10 L of purified water was injected.

③ Ginseng extract was prepared by heating ginseng and purified water mixture at 85 ~ 95 ℃.

5-2. Ginseng extract and Of the immune enhancing complex  mix

The ginseng extract prepared by the process of Production Example 5-1 and the immunostimulating complex prepared by the process of Production Example 4 were mixed.

Ginseng composition was prepared by mixing 80-120 parts by weight of ginseng extract on the basis of 100 parts by weight of the immune enhancing compound.

Depending on the conditions, the ginseng composition can be prepared using various formulations. For example, the ginseng extract may be prepared as a powder, the immune enhancing complex may be prepared as a powder, and the ginseng extract may be concentrated to prepare a ginseng concentrate and then mixed with the immune enhancing complex. Also, the ginseng concentrate may be prepared as a powder and the immune enhancing complex may be prepared as powder and mixed.

Manufacturing example  6. Preparation of ginseng composition : Ginseng inclusion compound and immunity enhancing complex  mix

6-1. Ginseng inclusion compound and Immune Enhancement Concentrate  mix

The gangue inclusion compound prepared by the process of Production Example 1, Production Example 2, Production Example 3 and the immunity enhancing composite prepared by the process of Production Example 4 were mixed.

Ginseng composition was prepared by mixing 80-120 parts by weight of ginseng extract on the basis of 100 parts by weight of the immune enhancing compound.

Depending on the conditions, the ginseng composition can be prepared using various formulations. For example, the ginseng extract may be prepared as a powder, the immune enhancing complex may be prepared as a powder, and the ginseng extract may be concentrated to prepare a ginseng concentrate and then mixed with the immune enhancing complex. Also, the ginseng concentrate may be prepared as a powder and the immune enhancing complex may be prepared as powder and mixed.

Reference Example  1. Ginseng Inclusion compound  Chromaticity analysis

1-1. Chromaticity analysis process

The Hunter's L value (whiteness), a value (redness), and b value (yellowness) of the ginseng inclusion compound were measured by using a colorimeter (CM-2500d. Visco) And repeatedly measured.

1-2. Chromaticity analysis result

Table 1 below shows the chromaticity of the ginseng inclusion compound 1 prepared by the process of preparation example 1, the phosphorus compound 2 prepared by the process of preparation example 2, and the ginseng inclusion compound 3 prepared by the process of preparation example 3 Table 1 shows the results. Each experimental group was reacted for 6 hours, 12 hours, and 24 hours.

sample Ginseng inclusion compound 1 Ginseng inclusion compound 2 Ginseng inclusion compound 3 6 hours 12 hours 24 hours 6 hours 12 hours 24 hours 6 hours 12 hours 24 hours L value 25.22 + - 0.33 24.68 ± 0.22 23.72 + - 0.55 54.92 + 0.79 52.14 ± 0.79 59.73 ± 0.79 71.54 + 1.05 73.29 ± 0.23 72.13 + - 0.01 a value 9.68 ±
0.94 9.02
0.97 9.43 ±
0.97 2.47 ±
0.99 2.73 ±
1.14 3.03 ±
1.17 5.45 ±
0.74 4.71 ±
0.74 4.86 ±
0.95 b value 21.95 ± 0.88 20.27 ± 0.95 21.59 ± 0.88 13.25 ± 1.29 13.24 0.94 15.42 ± 0.92 21.71 ± 0.92 18.52 + - 0.74 21.88 ± 0.88

As a result, whiteness of ginseng inclusion compound 3 prepared by distilled water was the highest, and ginseng inclusion compound 1 prepared by acetic acid and adjusted pH was generally lowest.

In addition, the red ginseng inclusion compound 3 was the highest in general and the ginseng inclusion compound 2 was the lowest in general.

Yellowness of ginseng inclusion compound 1 was the highest and ginsenoside inclusion compound 2 was the lowest. There was significant difference according to the composition of each ginseng inclusion compound, but there was no significant difference according to the production time of each ginseng inclusion compound.

As a result, the inclusion compound 3 of ginseng inclusion compound and the inclusion compound 2 of ginseng ethanol are generally applicable to food materials because of high whiteness degree. In addition, a method of controlling the chromaticity of ginseng inclusion compound 1 is additionally required.

Reference Example  2. Ginseng Inclusion compound  Sensory evaluation

Table 2 below is a table showing sensory evaluation of ginseng powder samples in which the bitter taste was removed by inversion with? -Cyclodextrin.

characteristic
(Charac -teristics)
Sample control 1 Ginseng inclusion compound 1 Ginseng inclusion compound 2 Ginseng inclusion compound 3 6h 12h 24h 6h 12h 24h 6h 12h 24h color
(Color) 3.70 ±
0.95 2.50
0.97 2.90
0.99 2.90
0.99 2.80
0.79 2.80
0.79 2.80
0.79 3.30 ±
1.34 3.20
1.32 3.30 ±
1.34 bitter
(Bitterness) 2.20
1.03 3.00 ±
1.41 2.80
1.23 2.60
1.27 2.70 ±
1.34 3.10
1.20 3.10
1.20 4.00 ±
0.94 4.00 ±
1.05 3.90
1.10 incense
(Flavor) 3.40 ± 0.97 2.00 0.94 2.50 + - 0.97 2.60 ± 0.97 3.10 ± 0.99 3.20 ± 1.14 3.40 ± 1.17 3.90 + - 0.74 3.90 + - 0.74 3.70 + - 0.95 flavor
(Taste) 2.50
1.27 2.00
1.25 2.40 ±
1.17 2.20
1.03 2.70 ±
0.95 2.50
1.18 2.50
1.18 3.80
1.03 4.00 ±
1.05 3.50
1.08 Overall likelihood
(Overall
acceptance) 2.70 ±
1.42 2.10
0.88 2.70 ±
0.95 2.10
0.88 2.90
1.29 3.00 ±
0.94 2.80
0.92 3.80
0.92 4.10 ±
0.74 3.90
0.88

(Control 1), ginseng inclusion compound 1 prepared by the process of preparation example 1, ginseng inclusion compound 2 prepared by the process of preparation example 2, ginseng inclusion compound 2 prepared by the process of preparation example 3 3 was evaluated. Each ginseng inclusion compound was reacted for 6 hours, 12 hours and 24 hours respectively.

Five items of color, bitterness, flavor, taste and overall acceptance of the powder were evaluated. The higher the score, the more the intensity of bitterness The evaluation method was formulated by weakening.

The preference of the untreated powder with no treatment was 3.70 ± 0.95, and the preference of ginseng inclusion compound 1 which was reacted for 6 hours was the lowest at 2.50 ± 0.97. The preference of ginseng inclusion compound 3, which was only reacted with distilled water, was generally high.

In the bitter taste item, the preference of ginseng inclusion compound 3 was higher than that of non - ginseng inclusion compound 3, and the preference of untreated powder was lowest.

In the fragrance items, the preference of ginseng inclusion compound 3 was high and the preference of ginseng inclusion compound 1 was the lowest.

In the overall preference item, the preference of the ginseng inclusion compound 3 was the highest as in the bitter taste item.

As a result, the bitter taste of ginseng inclusion compounds 1, 2 and 3 was all decreased. Among them, the ginseng inclusion compound 3 powder reacted only with water showed the highest bitter taste reduction efficiency of ginseng. Therefore, in case of ginseng inclusion compounds 1 and 2, an additional method of increasing the fragrance or preference degree is required.

Experimental Example  One. Ginseng inclusion compound Gin Senocide  Content measurement

1-1. Ginseng inclusion compound Gin Senocide  Content measurement process

The ginsenosides of all ginseng inclusion compounds were analyzed by the following method.

① 0.2 ~ 0.7g of ginseng inclusion compound powder sample was injected into a concentrating flask.

② Add 30 to 40 ml of water-saturated butanol to the concentrate flask and extract for 1 to 2 hours at 70 to 90 ° C on a reflux condenser.

③ A mixture of ginseng inclusion compound and water saturated butanol was placed for 7 ~ 15 minutes and cooled. The extract was filtered.

④ The same amount of saturated butanol was added to the remaining residue and extracted twice more.

⑤ Transfer the extract to a separating funnel, add 100 ~ 200 ml of distilled water, and shake.

⑥ Butanol layer and water layer were separated and the water layer was removed. The remaining butanol layer was collected in a concentrated flask and concentrated in vacuo using a rotary evaporator in a 40-50 water bath.

⑦ After concentration, the residue in the flask was dissolved in 10 ~ 30 ml of 40 ~ 60% MeOH and filtered with 0.45 membrane filter.

⑧ Gensenoside content was measured using Agilent 2690 HPLC system (Agilent Technologies, USA).

The HPLC analysis was carried out using a Bondapak C18 (3.9 mm 150 mm, 5) column. The flow rate and column temperature of the mobile phase as shown in Table 2 were set to 0.6 ml / min and 43, respectively, and the detection wavelength of the UV detector was determined to be 203 nm .

[Table 3] below is a table showing the solvent concentration gradient of the HPLC analysis program.

time (min) Acetonitrile (%) Water (%) 0 18 82 42 24 76 46 29 71 75 40 60 100 65 35 135 85 15 150 85 15 180 18 82

1-2. Ginseng Inclusion compound Gin Senocide  Content measurement result

Table 4 below shows the results of ginsenoside content measurement of the ginsenoside inclusion compound. Ginseng inclusion compound 1 was prepared by the process of Production example 1, and ginseng inclusion compound 2 was manufactured by the process of production example 2. In addition, the inclusion compound 3 of ginseng was prepared by the process of Production Example 3. As a control, ginseng concentrate was used.

Types of Gin Senocides Rg1 Re Rf Rb1 Rc Rb2 Rb3 Rd Rg6 F4 Rg3 Rk1 Rg5 Total amount Control group 0.86 1.01 1.92 2.56 6.53 2.73 0.24 2.96 1.57 1.16 3.78 1.98 3.98 31.71 Ginseng
Enclosure
Compound 1 - - 0.70 1.26 2.02 0.91 0.27 0.83 1.75 0.46 - - 0.24 8.45 Ginseng
Enclosure
Compound 2 0.64 0.64 1.32 1.81 4.44 1.94 0.50 2.12 1.14 0.84 1.33 0.17 0.32 17.35 Ginseng
Enclosure
Compound 3 0.49 0.49 0.99 1.08 2.61 1.05 0.31 0.82 0.74 0.63 0.18 - - 10.85

(Unit: mg / mL)

Total ginsenoside content of ginseng concentrate was 31.71 mg / mL, and it was 8.45 mg / mL in ginseng inclusion compound 1 with acetic acid. It was measured as 17.35 mg / mL in the ginseng inclusion compound 2 test group using ethanol as a solvent and 10.85 mg / mL in the ginseng inclusion compound 3 test group using distilled water as a solvent.

In addition, the product using ginseng has a higher ginsenoside content of the inclusion compound using the alcohol as the solvent in which the sum of Rg1 and Rb1 is measured to be as high as possible considering that the sum of ginsenosides Rg1 and Rb1 should contain 3 mg / mL or more Was measured at 2.45 mg / mL.

That is, when using the ginseng inclusion compound, it is preferable to adjust the concentration to satisfy the ginsenoside content standard.

Experimental Example  2. Of the immune enhancing complex Immune activity rate  Measure

2-1. Cell viability measurement

1) Cell viability measurement process

Macrophage is a tissue cell differentiated from blood mononuclear cells, and plays a protective role in the inflammation reaction. Macrophages are involved in the maintenance of immune homeostasis and play an important role in early infectious defense by producing cytokines such as nitric oxide (NO), tumor necrosis factor- (TNF-) and interleukin (IL-6)

One method for measuring the activity of macrophages is to quantitate the amount of NO (nitrogen monoxide) dissolved in the supernatant of cells in vitro. Quantification of the amount of NO is one of the important indirect functions of macrophages, which is indirectly related to phagocytosis. It is mainly used in conjunction with the MTT assay, and is often used to determine the initial optimal concentration of the immunologically active substance or to search for the initial active substance fraction.

To measure cell viability, RAW 264.7 cells were cultured and used for experiments. The RAW cell is a cell line that has been stabilized to grow and differentiate in a medium supplemented with appropriate serum. It is extracted from mouse laboratory animals such as Rat and Mouse. RAW 264.7 cells belonging to RAW cells are mouse macrophages.

The medium used for cell culture was DMEM (high glucose) medium supplemented with 10% FBS, penicillin (10, 000 IU / ml) and streptomycin (10, 000 g / ml) and subcultured every 4 days.

In addition, ELISA (Enzyme-Linked ImmunoSpecific Assay) is a method of measuring the amount of an antigen or an antibody by using an enzyme as a marker and using a serum immune response. EZ- Cell viability was measured using the cytox cell Viability Assay Kit. The measurement of cell viability is as follows.

① 96-well plate and then preparing six injected into the medium in each plate was inoculated 100 uL to be 5 × 10 ⁴ cell / well of RAW 264.7 cell, 4 ~ 6% CO 2, for 24 hours in a 37 incubator Lt; / RTI > One of the six plates was set as a control group and separated from the other plate.

(2) Immunostimulating concentrates were added to 5 plates except for the plate set as a control group in each of the cultured plates at a concentration of 25 ug / ml, 50 ug / lm, 100 ug / ml, 200 ug / ml and 400 ug / Lt; / RTI >

③ The absorbance of the control plate and the experimental plate was measured at 450 nm using the EZ-cytox cell Viability Assay Kit, and the cell viability was measured by comparing the OD of the control plate and the experimental plate. The cell survival rate was calculated based on the following formula (1).

2) Results of cell viability measurement

[Table 5] shows the result of measuring the cell survival rate by treating the RAW 264.7 cell with the immunoconjugate complex and the ginseng extract at different concentrations.

0 (ug / ml)
(Control group) 25 (ug / ml) 50 (ug / ml) 100 (ug / ml) 200 (ug / ml) 400 (ug / ml) Immune enhancement
Complex 100.00 ± 0.05 157.46 + 0.14 161.32 ± 0.08 154.22 0.08 142.80 + - 0.10 134.84 ± 0.06134.84 ± 0.06 Ginseng extract 100.00 0.08 167.32 + 0.07 163.47 ± 0.09 160.97 ± 0.16 164.99 + 0.11 172.80 ± 0.17

(unit : %)

 The absorbance of the control group without any treatment was set at 100% and compared with the absorbance of the experimental group. The cell viability was measured by adjusting the concentrations of the treated immune complexes to 25 μg / ml, 50 μg / ml, 100 μg / ml, 200 μg / ml and 400 μg / ml. The concentration of ginseng extract was treated in the same manner as the immune enhancing complex.

As a result, cell survival rate was higher in the immunocompromised composite test group and the ginseng extract test group than in the control group. The cell survival rate of the 50 ug / ml treated group was the highest at 161.32%, followed by 25 ug / ml, 100 ug / ml, 200 ug / ml and 400 ug / Respectively. Cell viability was found to be 157.46% at 25 ug / ml, 154.22% at 100 ug / ml, 142.80% at 200 ug / ml and 134.84% at 400 ug / ml. This can lead to the conclusion that the immune enhancing complex is cytotoxic regardless of concentration.

In the ginseng extract group, the cell survival rate of the treated group of 400 ug / ml was the highest at 172.80%, followed by the cell viability of 25 ug / ml, 200 ug / ml, 50 ug / ml and 100 ug / Respectively. The survival rate of 25 ug / ml was 167.32%, 200 ug / ml was 164.99%, 50 ug / ml was 163.47% and 100 ug / ml was 160.97%. Thus, it can be concluded that ginseng extract is not cytotoxic regardless of its concentration.

2-2. Macrophage NO  Production amount measurement

1) The process of measuring NO production of macrophages

1 is a graph showing an absorbance standard curve at 540 nm prepared using NaNO ₃ .

Nitric oxide (NO) produced by macrophages acts as an oxidative mechanism to kill external invading microorganisms and cancer cells, and to damage tissue damaged from infection. Therefore, it can be said that the amount of NO produced is proportional to the activity of macrophages.

NO ₂ ^- (nitrite), a stable NO oxide, was isolated from the monocytic cell line RAW 264.7 cell and measured by Griess reaction in culture supernatant. The Griess reagent is prepared with a composition of 0.05 to 0.2% N-1-naphthyl-ethylendiamine / H ₂ O: 0.5 to 2% sulfanilamide / 4 to 6% H ₃ PO ₄ = 1: 1. The process of measuring NO production is as follows.

① RAW 264.7 cell tissue was perfused with 50 mM potassium phosphate buffer containing EDTA (ethylenediamine tetra acetic acid).

(2) The supernatant was prepared by centrifuging the milled RAW 264.7 cell tissue at 3 ~ 5 ° C and 10, 000 × g for 15 minutes.

③ Twenty-four 96-well plates were prepared and divided into 4 immunoenhancement concentrate assay plates, 4 ginseng extract assay plates, and 10 immunoprecipitated complex assay plates. 100 L of centrifuged RAW 264.7 cell supernatant was applied to all plates Respectively.

④ Add 100 L of Griess reagent to the plate injected with supernatant and incubate for 10 minutes. Absorbance was measured at 540 nm with a microplate reader. The concentration of nitrite was calculated by comparing with the standard curve obtained using NaNO ₃ .

④ RAW 264.7 cells were reacted with the immunopotentiating concentrate for 24 hours, centrifuged at 2,500 rpm for 5 minutes, and the amount of NO was measured by taking 50 μl of the supernatant.

2) Results of NO production of macrophages

FIG. 2 shows the result of measurement of the amount of NO produced when the immune enhancing complex was treated with RAW 264.7 cells. FIG. 3 shows the results of measurement of the amount of NO produced when ginseng extract was treated with RAW 264.7 cells. FIG. 4 shows the results of measurement of NO production when RAW 264.7 cells were treated with the ginseng composition prepared by mixing the immunoconjugate complex and the ginseng extract.

As a result of the measurement, the amount of NO production was increased by increasing the concentration of the immunopotentiating complex to 50 μg / ml, 100 μg / ml, 200 μg / ml and 400 μg / ml. The NO production amount at all concentrations was significantly higher than the NO production amount in the control group. The NO production of the immunopotentiating complex increased from 50 μg / ml to 5.63 μM, from 100 μg / ml to 8.50 μM, from 200 μg / ml to 13.44 μM, and from 400 μg / ml to 16.14 μM. It can be seen that the immune enhancing complex increases the amount of NO produced in a concentration-dependent manner.

As the concentration of ginseng extract was also increased to 50 ug / ml, 100 ug / ml, 200 ug / ml and 400 ug / ml, the production of NO increased. At the concentration of 50 ug / ml, the value was lower than that of the control group. The NO production of all concentrations of ginseng extract was increased from 50 μg / ml to 0.66 μM, from 100 μg / ml to 0.91 μM, from 200 μg / ml to 1.69 μM and from 400 μg / ml to 1.78 μM. Ginseng extract increased the amount of NO production in a concentration dependent manner, but the amount of NO production was smaller than that of the immune enhancing complex.

In the ginseng composition prepared by mixing the immune enhancing complex and the ginseng extract, the amount of NO was different depending on the composition ratio of the immunopotentiating compound and the ginseng extract. NO production was the lowest at 0.82 uM when the composition of the immunopotentiating complex and ginseng extract was 0: 0, and the NO production was highest at 16.01 uM when the composition ratio of the immunopotentiating complex and ginseng extract was 200: 200.

That is, when the ginseng extract was reacted together with the immune enhancing complex alone, it was confirmed that NO production was more specifically increased at some concentrations. NO production of 15.25 uM was produced by treatment with 200 ug / ml of the immunoconjugate complex alone, but when treated with 200 ug / ml and 100 ug / ml of ginseng extract, 16.01 uM and 15.39 uM, respectively, resulted in NO production of 5% and 1 Respectively. Overall, it can be seen that NO production is promoted by the immune enhancing complex.

2-3. Immune cell Proliferative ability  Measure

1) Immunocyte proliferative activity measurement process

Jurkat cells, one type of T cells, and Jiyoye cells, one type of B cells, were used as immune cells for the measurement of immune cell proliferative capacity. Jurkat cells are infiltrating T lymphocytes and Jiyoye cells are Burkitt's lymphoma cells. All of them were distributed in Daejeon Biological Resource Center and used for experiments.

The medium used for cell culture was RPMI 1640 medium supplemented with 10% FBS, 1% penicillin or streptomycin, and subcultured every 4 days.

The MTT assay is a representative cell growth assay. MTT formazan (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide), which is a water-insoluble substrate of yellow color due to the dehydrogenase action of living cell mitochondria, This phenomenon does not occur in the cytoplasm.

The process of measuring immune cell proliferative activity is as follows.

① Five 96-well plates were prepared and RPMI 1640 medium was injected into all plates.

T cells and B cells were treated with 1 × 10 ⁵ cells / well in all plates.

③ Immunostimulating concentrates were treated with 25 ug / ml, 50 ug / ml, 100 ug / ml, 200 ug / ml and 400 ug / ml on four plates except for one plate set as a control group.

④ The plate treated with the immune enhancement concentrate was reacted for 48 hours and measured by MTT assay.

2) Results of measurement of immune cell proliferative capacity

Table 6 below shows the results of measuring the growth of immune cells by treating immune enhancing complexes and ginseng extracts on Jurkat cells and Jiyoye cells at different concentrations.

0 (ug / ml)
(Control group) 25 (ug / ml) 50 (ug / ml) 100 (ug / ml) 200 (ug / ml) 400 (ug / ml) Jurkat
Immune enhancement
Complex 100.0 + - 0.01 91.49 + - 0.01 89.67 ± 0.01 85.34 + - 0.01 87.32 ± 0.01 77.93 + 0.03 Ginseng
extract 100.0 ± 0.05 85.69 ± 0.01 81.25 ± 0.01 76.62 ± 0.00 73.68 ± 0.02 74.92 + 0.03 Jiyoye
Immune enhancement
Complex 100.0 + - 0.01 119.13 ± 0.00 105.03 + 0.04 101.76 ± 0.05 94.44 + 0.04 89.97 0.02 Ginseng
extract 100.0 + - 0.01 127.64 + 0.07 119.63 ± 0.02 131.37 ± 0.09 134.93 + 0.04 100.77 + 0.02

(unit : %)

The absorbance of the control group without any treatment was set at 100% and compared with the absorbance of the experimental group. The concentrations of immune enhancing complexes were adjusted to 25 ug / ml, 50 ug / ml, 100 ug / ml, 200 ug / ml and 400 ug / ml, and the concentration of ginseng extract was also treated in the same manner as the immune enhancing complex.

The results showed that the immune - enhancing complex and ginseng extract decreased the growth of Jurkat cells but increased the growth of Jiyoye cells. When the immune enhancing complex was treated with Jiyoye cells, the concentration of 25 ug / ml was 119.13%, 50 ug / ml was 105.03%, 100 ug / ml was 101.76%, 200 ug / ml was 94.44% and 400 ug / ml was 89.97% Respectively. It was confirmed that all groups except 200 ug / ml and 400 ug / ml increased the growth rate by at least 1%.

When the extract of Ginseng was treated with Jiyoye cells, the concentration of 25 ug / ml was 127.64%, 50 ug / ml was 119.63%, 100 ug / ml was 131.37%, 200 ug / ml was 134.93% and 400 ug / ml was 100.77% Respectively. It can be confirmed that the growth rate was increased by at least 0.7% in all experimental groups.

Thus, it can be concluded that ginseng extract and immune-enhancing complexes generally promote the growth of B cells.

2-4. Total polyphenol content and total flavonoid content

5 is a graph showing a standard curve of total polyphenol contents prepared using gallic acid. 6 is a graph showing a standard curve of the total flavonoid content prepared using quercetin.

1) Total polyphenol content measurement

Benzene (C ₆ H ₆₎ one of the hydrogens of the ring is a hydroxyl group (-OH) of the group, hydroxy is called phenolics 2 '(多價) a polyhydric phenol, at least the material that has substituted with, i.e. poly It is called a polyphenol.

Polyphenol is a biodegradable material possessed by plants to protect themselves from external harmful factors such as pigment, bitter taste, and bitter component contained in the skin, epidermis, and seeds of plants, and active oxygen generated by ultraviolet rays. Examples of polyphenols are catechins contained in green tea, chlorogenic acid contained in coffee, red and purple anthocyanin pigments such as strawberry, eggplant, grape, black bean, and red bean.

Polyphenols also act as antioxidants in the human body. It is also known to enhance immune function and to be effective for skin whitening and fatigue recovery.

Total polyphenol content measurements were performed according to the Singleton-Rossi (1965) method. Folin-Ciocalteu reagent was reduced by molybdenum blue color by the phenolic compound of the sample, and the total polyphenol content was calculated by a standard curve prepared using gallic acid as a standard material. The method is as follows.

① One 96-microwell plate was prepared, 20 μl of the immune enhancing complex was injected, 100 μl of 15-25% sodium carbonate was mixed and the mixture was allowed to stand at room temperature for 5 minutes.

② 100 μl of 1.0 N Folin-Ciocalteu reagent was added, reacted at room temperature for 1 hour, and absorbance was measured at 720 nm using a microplate reader. Total polyphenol content was calculated based on the standard curve prepared using gallic acid.

2) Total flavonoid content measurement

Flavonoids are widely distributed in food, and have a flavonoid as a basic structure. The flavonoids have two phenyl groups, C ₃ It is composed of a carbon skeleton bonded through a chain. It is safe in acidity, and the color becomes clearer. In strong alkalis, however, its structure changes to dark yellow or brown. It has antibacterial, anticancer, antiviral, antiallergic and anti-inflammatory effects, and it is reported that there is almost no toxicity. It is also known as an antioxidant like polyphenol, and it is said to enhance the immune function and to be effective for skin whitening and fatigue recovery.

Total flavonoid content was measured by spectrophotometer in Bao et al. (2005). The method is as follows.

① 70 ~ 80% methanol was added to 0.1 g of the immune enhancing complex, and the mixture was extracted at room temperature for 24 hours.

② 1.0 ml of the extract of the immune enhancing complex was taken in a test tube and 10 ml of diethylene glycol was mixed.

③ 0.1 ml of 1 N NaOH was mixed with the mixture solution of immune enhancing complex and diethylene glycol, reacted for 1 hour in a water bath at 35 ~ 40 ℃, and the absorbance was measured at 420 nm. For comparison, a 40-60% methanol solution was treated identically instead of the immune enhancing complex, and a standard curve was generated using quercetin.

3) Total polyphenol and total flavonoid content

Table 7 below shows the results of measuring the total polyphenol content and total flavonoid content of the immune enhancing compound and the ginseng extract.

Total polyphenol content
Total flavonoid content Immune enhancing complex 39.03 + - 1.72 7.11 + - 0.45 Ginseng extract 37.97 ± 1.48 10.33 + - 0.35

(Unit: mg / g)

The total polyphenol content of the ginseng extract was 37.07 mg / g, the total flavonoid content was 10.33 mg / g, and the total polyphenol content and total flavonoid content of the immune enhancing complex were 39.03 mg / g and 7.11 mg / g, respectively. g.

The total polyphenol content of the immunopotentiating complex is lower than the total polyphenol content of the ginseng extract and the total flavonoid content of the immunopotentiating complex is higher than the total flavonoid content of the ginseng extract, but both contain a high content of polyphenols and flavonoids.

This suggests that the immune-enhancing complex and ginseng extract contain large amounts of polyphenols and flavonoids.

Claims (5)

delete 60 to 90 parts by weight of peanut, 50 to 80 parts by weight of licorice, 10 to 30 parts by weight of licorice, 40 to 70 parts by weight of ginger and 40 to 70 parts by weight of jujube are mixed and extracted to prepare an immune enhancing complex ;
Preparing a ginseng inclusion compound by reacting? -Cyclodextrin in an amount of 2.5 to 3.5 parts by weight based on 1 part by weight of ginseng concentrate or ginseng powder;
And mixing 80 to 120 parts by weight of the ginseng inclusion compound on the basis of 100 parts by weight of the immunostimulating complex.
The method of claim 2,
Wherein the concentration of the immunostimulating complex is 25 to 400 ug / ml.
A ginseng composition prepared by the method of claim 2.
A food functional additive added to a ginseng composition prepared by the method of claim 2,

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