CN112843153A - Nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity, and preparation method and application thereof - Google Patents
Nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity, and preparation method and application thereof Download PDFInfo
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- CN112843153A CN112843153A CN202110303677.2A CN202110303677A CN112843153A CN 112843153 A CN112843153 A CN 112843153A CN 202110303677 A CN202110303677 A CN 202110303677A CN 112843153 A CN112843153 A CN 112843153A
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Abstract
The invention provides a nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity, which comprises the following components in parts by weight: 75-85 parts of honey, 1-10 parts of royal jelly and 10-15 parts of a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 30-40 parts of acanthopanax senticosus, 10-30 parts of polygonum cuspidatum, 10-20 parts of folium isatidis, 5-15 parts of scutellaria baicalensis, 5-15 parts of fructus forsythiae, 5-15 parts of sculellaria barbata and 5-15 parts of liquorice, and can effectively inhibit the development and propagation of pathogenic bacteria, enhance the disease resistance of bees, supplement nutrition required by bee breeding, effectively treat diseases such as bee rotten son disease and the like, and enhance the breeding and feeding capacity of the bees. The invention also provides a preparation method, application and application method of the traditional Chinese medicine nutrient solution, which can exert the drug effect to the maximum extent, improve the curative effect of treating bee rotten son disease and enhance the breeding and feeding capacity of bees.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicines, in particular to a nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity, and a preparation method and application thereof.
Background
Bee brood is also called bee sacbrood, is a bee viral infectious disease which is commonly generated in the world, is commonly serious in Chinese bees in China, is caused by larval bacillus, is bred in rotten larvae, is difficult to treat once suffering from the disease, and is spread by nurturing other larvae through worker bees. Generally, the disease is serious in spring and autumn, and once the disease is infected, the disease is easy to form epidemic because of strong infectivity of pathogenic bacteria, the disease is fast to happen, so that bee colonies cross the colony, fly away or die in the whole colony, and destructive loss is caused. At present, no specific medicine is used for radical treatment, antibiotic medicines such as chloramphenicol and the like are generally used for treatment at present, the symptoms are treated but the root causes are not treated, the rotten son disease is easy to repeatedly attack, and the antibiotic can not be decomposed automatically and is easy to remain in bee products, so that the quality and the safety of the bee products are influenced.
Chinese patent with application number CN201210549180.X discloses a pure traditional Chinese medicine composition for treating bee sacbrood, which is prepared from the following raw material medicines in parts by weight: 25-45 parts of cyrtomium rhizome, 25-35 parts of honeysuckle, 15-35 parts of liquorice, 15-35 parts of cortex acanthopanacis, 25-35 parts of sculellaria barbata, 15-35 parts of fructus forsythiae and 15-35 parts of polygonum cuspidatum. Firstly, although the technical scheme has a certain treatment effect, the root is difficult to break, the process is easy to repeat, and the effect of preventing the rotten son disease of the bees in advance cannot be achieved, so that economic loss is brought to beekeepers to a certain extent; in addition, in the treatment process, the technical scheme cannot improve the favorite food ability of the bees, so that the treatment effect cannot be well achieved, and the nutrition supply to bee colonies cannot be met, so that on one hand, the technical scheme cannot rapidly achieve the effects of preventing and treating bee brood, and on the other hand, the technical scheme ignores the breeding and feeding conditions of the bees, and cannot achieve the effect of improving the breeding and feeding capacity of the bees.
Disclosure of Invention
The first purpose of the invention is to provide a traditional Chinese medicine nutrient solution for preventing and treating bee brood, unifying group taste and enhancing feeding capacity, which can effectively inhibit the development and propagation of pathogenic bacteria, enhance the disease resistance of bees, supplement nutrition required by bee breeding, effectively treat diseases such as bee brood and the like, and enhance the breeding and feeding capacity of the bees.
The second purpose of the invention is to provide a preparation method, application and application method of the traditional Chinese medicine nutrient solution for preventing and treating bee brood, unifying group taste and enhancing feeding capacity, which can exert the drug effect to the maximum extent, improve the curative effect of treating bee brood and enhance the bee breeding and feeding capacity.
The embodiment of the invention is realized by the following technical scheme:
a traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity comprises the following components in parts by weight: 75-85 parts of honey, 1-10 parts of royal jelly and 10-15 parts of a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 30-40 parts of acanthopanax senticosus, 10-30 parts of polygonum cuspidatum, 10-20 parts of folium isatidis, 5-15 parts of scutellaria baicalensis, 5-15 parts of fructus forsythiae, 5-15 parts of sculellaria barbata and 5-15 parts of liquorice.
Application of a Chinese medicinal nutritional liquid in preparing medicine for preventing and treating bee brood, unifying colony taste, safely merging bee colony and queen bee, and enhancing bee breeding and feeding ability is provided.
A preparation method of a traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity comprises the following steps:
1) weighing the components according to the proportion, cleaning, crushing into medium-coarse powder, adding water and decocting for at least 2 times;
2) mixing the liquid medicines obtained after multiple times of decoction, filtering, standing and concentrating to obtain a traditional Chinese medicine preparation;
3) cooling the decocted Chinese medicinal preparation, adding honey and royal jelly, uniformly stirring, and freezing and storing at the temperature of between 15 ℃ below zero and 20 ℃ below zero.
A method for applying a traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity comprises the following steps: feeding or spraying the spleen with 40-50 g of Chinese medicinal nutrient solution every evening, feeding or spraying the spleen once every other day, and repeatedly feeding or spraying the spleen for 4-5 times.
The technical scheme of the embodiment of the invention at least has the following advantages and beneficial effects:
1. the traditional Chinese medicine nutrient solution of the invention combines various medicines, and has the effects of promoting qi circulation, consolidating constitution, clearing away heat and toxic material, and promoting bee reproduction and larva growth and development; the Chinese medicinal nutrient solution can effectively inhibit the development and propagation of pathogenic bacteria, enhance the disease resistance of bees, supplement nutrients required by bee breeding, effectively treat bee brood and other diseases, and most importantly, the Chinese medicinal nutrient solution can greatly enhance the breeding and feeding capacity of the bees, can be safely and effectively used for mutual combination of bee colonies and intervention of queen loss colonies in queen bees, and keeps the bee colonies quiet and stable.
2. The traditional Chinese medicine nutrient solution disclosed by the invention utilizes pure natural Chinese herbal medicines, honey and royal jelly, has no chemical composition and antibiotic components, has no residues, is pollution-free, green and environment-friendly, has no drug residues on bee products, meets the production safety requirements of pollution-free foods, and can solve the problems that the root breaking, the repetition and the antibiotic residue in the bee products are difficult and the like when the chemical antibiotic medicines are applied to treat bee rotten son diseases in the prior art.
3. The ointment is decocted for three times during decoction, and is decocted in a gradient manner from high temperature to low temperature, so that on one hand, the decocted medicine is prevented from being scorched, and the medicine effect is exerted most quickly; on the other hand, the Chinese herbal medicines are boiled by small fire, the royal jelly and the honey are added, the mixture is uniformly stirred, and the mixture is placed into a refrigerator for freezing storage, so that the Chinese herbal medicines and the effective components in the royal jelly can be effectively stored for a long time, the medicinal components are highly matched with the honey and the royal jelly, higher medicinal effect is exerted, the bee is easy to absorb and digest, the nutrition is rich, the bee feeding capacity is greatly enhanced, the characteristics of the bee like eating are met, the production process is simple and reasonable, and the large-scale industrial production is easy.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity, the preparation method, the use method and the application thereof provided by the embodiment of the invention are specifically described below.
A traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity comprises the following components in parts by weight: 75-85 parts of honey, 1-10 parts of apis mellifera royal jelly and 10-15 parts of a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 30-40 parts of acanthopanax senticosus, 10-30 parts of polygonum cuspidatum, 10-20 parts of folium isatidis, 5-15 parts of scutellaria baicalensis, 5-15 parts of fructus forsythiae, 5-15 parts of sculellaria barbata and 5-15 parts of liquorice.
Preferably, the paint comprises the following components in parts by weight: 80-85 parts of honey, 4-8 parts of royal jelly and 10-15 parts of a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 35-40 parts of acanthopanax senticosus, 15-25 parts of polygonum cuspidatum, 15-20 parts of folium isatidis, 5-10 parts of scutellaria baicalensis, 5-10 parts of fructus forsythiae, 5-10 parts of sculellaria barbata and 10-15 parts of liquorice.
Preferably, the paint comprises the following components in parts by weight: 80 parts of honey, 5 parts of apis mellifera royal jelly and 15 parts of a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 35 parts of acanthopanax senticosus, 20 parts of polygonum cuspidatum, 15 parts of folium isatidis, 10 parts of scutellaria baicalensis, 10 parts of fructus forsythiae, 10 parts of sculellaria barbata and 10 parts of liquorice.
Preferably, the paint comprises the following components in parts by weight: 80 parts of honey, 10 parts of apis mellifera royal jelly and 10 parts of a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 30 parts of acanthopanax senticosus, 15 parts of polygonum cuspidatum, 20 parts of folium isatidis, 15 parts of scutellaria baicalensis, 5 parts of fructus forsythiae, 10 parts of sculellaria barbata and 10 parts of liquorice.
Acanthopanax senticosus, named as follows: radix Et caulis Acanthopanacis Senticosi, KANGZHULING, and WUJISHEN. Pungent, slightly bitter and warm in nature; spleen, kidney and heart meridians entered; contains 4-methyl salicylaldehyde, tannin, palmitic acid, linolenic acid, vitamin A, vitamin B and the like, has better adaptability, can enhance the body resistance, regulate the pathological process and the like, and has the functions of resisting inflammation, resisting bacteria and resisting viruses; the functions are mainly used for replenishing qi to invigorate the spleen, tonifying the kidney and soothing the nerves.
Giant knotweed rhizome: polygonaceae, a medicinal plant of Asclepiadaceae, with root-like stem, slightly bitter and cold in nature and taste; it enters liver, gallbladder and lung meridians. Has effects of promoting blood circulation, removing blood stasis, regulating qi-flowing, and relieving pain. Contains emodin, glucoside and several polysaccharides, and has heat-clearing, detoxicating, antibacterial, and antivirus effects.
Folium isatidis: is dried leaf of Isatis tinctoria L of Isatis of Brassicaceae. Bitter taste and cold nature; it enters liver, heart, stomach and spleen meridians. Folium Isatidis contains tryptophan, indirubin B, glucose brassin, new glucose brassin, glucose brassin-1-sulfonate, and indigo. Has the effects of clearing away heat and toxic materials, cooling blood and removing ecchymoses. Mainly treats heat entering nutrient blood, warm poison and macula, sore throat and aphtha, mumps and erysipelas.
Radix scutellariae: labiatae, bitter in taste and cold in nature. It enters lung, gallbladder, spleen, large intestine and small intestine meridians. Has effects of clearing heat, eliminating dampness, clearing pathogenic fire, removing toxic substances, stopping bleeding, preventing miscarriage, relieving tension and uneasiness, and stabilizing physical condition, and can be used for treating chest distress, nausea, damp-warm syndrome, summer-warm syndrome, abdominal distention, dysentery, jaundice, cough due to lung heat, hyperpyrexia, polydipsia, blood heat, hematemesis, carbuncle, suppurative sore, and threatened abortion; the pharmacological action of the baicalin is as follows: antiinflammatory, antibacterial, antiviral, tranquilizing, and heat and toxic materials clearing away effects. The radix Scutellariae contains baicalein, baicalin, wogonin and baicalein, and also contains benzoic acid, beta-sitosterol, etc. The stem and leaf contain baicalin. The main components are as follows: flavones and tannins.
Fructus forsythiae: bitter in nature and cool; it enters heart, liver and gallbladder meridians. The functional indications are as follows: clear heat, remove toxicity, dissipate nodulation and resolve swelling. It is indicated for warm heat, erysipelas, macula, superficial infection, abscess, swelling, scrofula and stranguria.
Barbed skullcap herb: pungent, bitter and cold in nature; clearing away heat and toxic material, dispersing blood stasis and promoting urination. The herba Scutellariae Barbatae has effects in clearing away heat and toxic materials, promoting blood circulation, dispelling blood stasis, relieving swelling and pain, and resisting cancer. The whole herb contains various vitamins, microelements and amino acids. Has the effects of cooling blood, removing toxic substances, removing blood stasis, relieving pain, relieving swelling, clearing heat and promoting diuresis. The barbed skullcap herb contains: carthamin, isocarthamin, alpine baicalein, alpine chalasin, beta-sitosterol, stearic acid, alkaloid polysaccharide and the like.
Licorice root: sweet and mild in nature and taste; it enters heart, lung, spleen and stomach meridians. Invigorating spleen and replenishing qi, clearing away heat and toxic material, eliminating phlegm and relieving cough, relieving spasm and pain, and harmonizing the drugs. Can be used for treating weakness of spleen and stomach, asthenia, palpitation, short breath, cough, excessive phlegm, abdominal and limb spasm, pain, carbuncle, swelling, sore, and drug toxicity and strong nature.
Honey: the bee nutrition liquid is rich in glucose, fructose, protein, amino acid, vitamin, trace elements, active enzyme and the like, has the functions of promoting gastrointestinal digestion, strengthening physique, promoting bee reproduction, improving body resistance, improving the taste of Chinese herbal medicine nutrient solution and increasing the favorite characteristics of bees.
Apis mellifera royal jelly: contains a large amount of proteins, sugars, fats, lipid substances and inorganic compounds. The chemical composition is roughly as follows: 65% of water, 12% of protein, 6% of fat, 13% of carbohydrate and 1% of ash. The royal jelly contains amino acids required by bee growth, vitamins mainly including B vitamins, 7-9 sterol compounds, acetylcholine, sphingomyelin, phosphatidylethanolamine, glucose oxidase, amylase, cholinesterase, epinephrine and trace elements, and can promote the growth of bee larva.
In the Chinese herbal medicine nutrient solution composition, acanthopanax senticosus and scutellaria baicalensis are mainly used for promoting the circulation of qi to clear away heat and toxic materials, consolidate the constitution, enhance the body resistance, prevent miscarriage and improve the fertility and are used as monarch drugs; the giant knotweed rhizome and the dyers woad leaf are used as ministerial drugs to treat principal symptoms and concurrent symptoms, so that the immunity and the antiviral effect of the bee organism can be greatly improved, and the honeybee colony can be rejuvenated and rapidly propagated after diseases, and has a good promoting effect; fructus forsythiae and herba Scutellariae Barbatae are used as adjuvant drugs for clearing away heat and toxic materials, and assisting the monarch and minister drugs in enhancing the treatment effect; the liquorice is used for harmonizing the medicines, so that the medicines are sweet in taste, bees like to eat, the honey promotes the propagation of the bees and improves the body resistance, the taste of the Chinese herbal medicine nutrient solution can be improved, the bees like to eat, and the royal jelly can promote the growth and development of bee larvae. The medicines are combined, the monarch and minister medicines cooperate with each other, and the assistant and guide medicines cooperate with each other to play the effects of promoting qi circulation, consolidating constitution, clearing away heat and toxic materials, preventing miscarriage and promoting bee reproduction and larva growth and development. The bee nutrient solution can effectively inhibit the development and propagation of pathogenic bacteria, enhance the disease resistance of bees, supplement nutrient components such as various vitamins, trace elements, amino acids and the like required by bee breeding, is suitable for treating diseases such as bee brood and the like, and simultaneously enhance the breeding and feeding capacity of the bees.
In addition, after the components of the nutrient solution are decocted, particularly the medicinal flavors of acanthopanax senticosus and polygonum cuspidatum are stimulated, so that the nutrient solution has very strong volatile medicinal flavors, like the cool feeling of mint, the nutrient solution can greatly promote bees to lick mutually, and further promote different bee colonies to have the same smell, thereby stimulating the mutual identification and combination of different bee colonies, promoting the intervention of queen bees, improving the reproductive capacity of the bee colonies, and providing reliable guarantee for later-stage honey collection.
A preparation method of a traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity comprises the following steps:
1) weighing the components according to the proportion, cleaning, crushing into medium-coarse powder, adding water and decocting for at least 2 times;
2) mixing the liquid medicines obtained after multiple times of decoction, filtering, standing and concentrating to obtain a traditional Chinese medicine preparation;
3) cooling the decocted Chinese medicinal preparation, adding honey and royal jelly, uniformly stirring, and freezing and storing at the temperature of between 15 ℃ below zero and 20 ℃ below zero.
Further, during the decoction in the step 1), the decoction is carried out for three times: a first boiling stage: decocting at 80-100 deg.C for 30-60min, and filtering to obtain medicinal liquid; a second boiling stage: decocting at 60-80 deg.C for 30-60min, and filtering to obtain medicinal liquid; a third boiling stage: decocting at 40-60 deg.C for 10-30min, and filtering to obtain medicinal liquid.
The invention is decocted for three times, and is decocted in a gradient manner from high temperature to low temperature, on one hand, the decocted medicine is prevented from scorching and becoming charcoal without scorching, so that the medicine can exert the effect at the fastest speed; on the other hand, the Chinese herbal medicines are boiled by small fire, the royal jelly and the honey are added, the mixture is uniformly stirred, and the mixture is placed into a refrigerator for freezing storage, so that the Chinese herbal medicines and the effective components in the royal jelly can be effectively stored for a long time, the medicinal components are highly matched with the honey and the royal jelly, higher medicinal effect is exerted, the bee is easy to absorb and digest, the nutrition is rich, the bee feeding capacity is greatly enhanced, the characteristics of the bee like eating are met, the production process is simple and reasonable, and the large-scale industrial production is easy.
Further, cooling the decocted traditional Chinese medicine preparation to below 40 ℃ in the step 3), then adding honey and royal jelly, uniformly stirring, and freezing and storing at the temperature of between 15 ℃ below zero and 20 ℃ below zero.
Application of a Chinese medicinal nutrient solution in preparing medicine for preventing and treating bee brood and enhancing bee feeding ability is provided.
A method for applying a traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity comprises the following steps: feeding or spraying the spleen with 40-50 g of Chinese medicinal nutrient solution every evening, feeding or spraying the spleen once every other day, and repeatedly feeding or spraying the spleen for 4-5 times.
After the traditional Chinese medicine nutrient solution is used, a smooth medicine fragrance is sprayed in the beehive, and the traditional Chinese medicine nutrient solution has a good effect of attracting bees, so that the traditional Chinese medicine nutrient solution can attract the bees to eat and can better exert the medicine effect on the bees.
Further, for already diseased bee colonies: the bee colony is tightened, the bee quantity is compacted, the bee colony is taken to a distant place for isolation, the diseased son spleen is extracted to be burnt out, and the queen bee is closed to prevent escape.
Example 1
A Chinese medicinal nutrient solution for preventing and treating bee brood, unifying group taste and enhancing feeding power comprises: 1000g of honey, 50g of royal jelly, 35g of acanthopanax senticosus, 20g of giant knotweed, 15g of folium isatidis, 10g of radix scutellariae, 10g of fructus forsythiae, 10g of sculellaria barbata and 10g of liquorice;
a preparation method of a traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity comprises the following steps: pulverizing the above Chinese medicinal materials into coarse powder; decocting in water for 3 times, mixing the medicinal liquids obtained in 3 times, filtering, precipitating, and making into Chinese medicinal preparation; cooling the decocted Chinese herbal preparation to below 40 deg.C, adding Mel and Lac Regis Apis, stirring, and freezing in refrigerator for storage; and (5) subpackaging for later use after the inspection is qualified.
A method for applying a traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity comprises the following steps: feeding or spraying 40g of Chinese medicinal nutrient solution to spleen at evening, feeding or spraying spleen once every other day, and repeatedly feeding or spraying spleen for 5 times; for already diseased bee colonies: the bee colony is tightened, the bee quantity is compacted, the bee colony is taken to a distant place for isolation, the diseased son spleen is extracted to be burnt out, and the queen bee is closed to prevent escape.
When the traditional Chinese medicine nutrient solution is used for treating bee brood, the traditional Chinese medicine nutrient solution is poured into a feeder of a sick bee colony, the amount of the traditional Chinese medicine nutrient solution corresponding to each frame of bees is 50g, and the feeding is carried out for 1 time every other day and for 5 times continuously.
When the traditional Chinese medicine nutrient solution is used for preventing bee brood, the traditional Chinese medicine nutrient solution is poured into a feeder of a bee colony, and the amount of the traditional Chinese medicine nutrient solution corresponding to each frame of bees is 40g, and the traditional Chinese medicine nutrient solution is used once a month.
When the traditional Chinese medicine nutrient solution is used for accelerating bee breeding, the traditional Chinese medicine nutrient solution is poured into a feeder of a bee colony, the amount of the traditional Chinese medicine nutrient solution corresponding to each frame of bees is 30g, and the feeding is carried out once every three days and is carried out for 5 times continuously.
Example 2
A Chinese medicinal nutrient solution for preventing and treating bee brood, unifying group taste and enhancing feeding power comprises: 1500g of honey, 100g of royal jelly, 30g of acanthopanax senticosus, 15g of giant knotweed, 20g of folium isatidis, 15g of radix scutellariae, 10g of fructus forsythiae, 10g of sculellaria barbata and 10g of liquorice;
the preparation method of the Chinese medicinal nutrient solution of the embodiment is the same as that of the embodiment 1.
The method of administering the nutrient solution of Chinese herbs in this example is the same as that in example 1.
Example 3
A Chinese medicinal nutrient solution for preventing and treating bee brood, unifying group taste and enhancing feeding power comprises: 750g of honey, 55g of royal jelly, 30g of acanthopanax senticosus, 10g of giant knotweed, 20g of folium isatidis, 15g of radix scutellariae, 15g of fructus forsythiae, 15g of sculellaria barbata and 15g of liquorice;
the preparation method of the Chinese medicinal nutrient solution of the embodiment is the same as that of the embodiment 1.
The method of administering the nutrient solution of Chinese herbs in this example is the same as that in example 1.
Example 4
A Chinese medicinal nutrient solution for preventing and treating bee brood, unifying group taste and enhancing feeding power comprises: 900g of honey, 50g of royal jelly, 40g of acanthopanax senticosus, 10g of giant knotweed, 20g of folium isatidis, 5g of radix scutellariae, 5g of fructus forsythiae, 5g of sculellaria barbata and 10g of liquorice;
the preparation method of the Chinese medicinal nutrient solution of the embodiment is the same as that of the embodiment 1.
The method of administering the nutrient solution of Chinese herbs in this example is the same as that in example 1.
Experimental example 1
(1) Test of efficacy in treating bee brood
Dividing Chinese bees suffering from the rotten son disease into four groups according to the number of the bees, wherein the four groups are respectively as follows: experimental group, comparative group 1, comparative group 2, blank group, group to group sufficient distance, will not be mutual infection.
Experimental groups: the group of bee colonies are fed with the traditional Chinese medicine nutrient solution of the embodiment 1, and the feeding method comprises the following steps: the Chinese medicinal nutrient solution of the embodiment 1 is poured into a feeder of sick bee colonies, the amount of each frame of bees corresponding to the Chinese medicinal nutrient solution of the embodiment 1 is 50g, the feeding is carried out for 1 time every other day, and the feeding is carried out for 3-5 times continuously.
Comparative group 1: the bee colony is fed according to a pure traditional Chinese medicine composition for treating the sacbrood disease of the bees, which is disclosed in Chinese patent No. CN201210549180.X.
Comparative group 2: the group of bee colonies are fed with chloramphenicol by the following feeding method: chloromycetin is added into white sugar water to attract bees to eat, and 0.25g of amoxicillin is fed to 20 swarm bees.
Blank group: the swarms of the group were not fed with any drug.
The number of deaths of four groups of worker bees was recorded after 15 consecutive days of observation, and the statistical data are shown in table 1.
TABLE 1 number of deaths from four groups of worker bees for the treatment of bee rotten son disease
As can be seen from table 1:
a. the number of deaths in the four groups at the beginning of drug use (day 2 of the test) was comparable, indicating that no drug effect was apparent.
b. In the middle stage of drug use (test day 5, test day 10), the drug effects of the test group, the control group 1 and the control group 2 are shown, the death number of the test group is suddenly reduced from 120 to 18, and the drug effects are excellent and remarkably effective compared with those of the control group and the blank group; although the death numbers of the comparative group 1 and the comparative group 2 are reduced slightly, the death numbers rise back to the 10 th day of the experiment, which shows that the traditional Chinese medicine composition of the comparative group 1 and the amoxicillin of the comparative group 2 are used, the symptoms are treated, the root causes are not treated, and the sick bee colony generates drug resistance.
c. By the end of the drug application (day 16 of the test), the test group had no longer produced dead worker bees, indicating a complete cure of the rotten son disease of the colony.
The data of the experimental group show that the death number of worker bees is continuously reduced from the 2 nd day of the experiment without rebounding, and the death number is reduced to 1/10 at the beginning by the 10 th day, which shows that the threat of the rotten son disease to the sick bee colony is relieved, and only the 15 th day is spent from the treatment to the cure, thereby showing that the traditional Chinese medicine nutrient solution has quick drug effect and good treatment effect. The more quickly the rotten son disease is treated, the more the bee colony can be preserved, the more the loss can be saved, and the cost is reduced. Compared with the prior art, by the 10 th day, the death number of the comparison group 1 is reduced more and more slowly, so that the drug effect is slow to play, even the drug effect is almost completely played, the treatment effect on the rotten son disease is not good, and the quick radical treatment cannot be realized; the number of deaths of the comparative group 2 is greatly increased, and the number of deaths is half of that of the blank group, so that the treatment effect is poor.
In conclusion, the Chinese medicinal nutrient solution for treating bee rotten son disease has quick response, stable and continuous medicinal effect and short curing time, adopts natural Chinese medicinal materials, has no medicament residue on bee products, and meets the production safety requirement of nuisanceless food.
Experimental example 2
(2) Test for efficacy in preventing bee brood
Four healthy Chinese bee fields are selected and divided into four groups, which are respectively: experimental group, comparative group 1, comparative group 2, blank group, group to group sufficient distance, will not be mutual infection.
Experimental groups: the bee space of the group is provided with a bee 39 spleen, and the bee colony is fed with the traditional Chinese medicine nutrient solution of the embodiment 2, and the feeding method comprises the following steps: the traditional Chinese medicine nutrient solution of the embodiment 2 is poured into a feeder of a bee colony, the amount of the traditional Chinese medicine nutrient solution put into each frame of bees is 30g, and the traditional Chinese medicine nutrient solution is put into the feeder for 1 time in one month.
Comparative group 1: the bee field is fed with a pure traditional Chinese medicine composition for treating the sacbrood disease of bees, which is disclosed in Chinese patent CN201210549180. X.
Comparative group 2: the bee field of the group is provided with a bee 40 spleen, and the bee colony is fed with amoxicillin by the feeding method: the amoxicillin is mixed into white sugar water with the ratio of 1:1 to attract bees to eat, and 0.25g of amoxicillin is fed to 12-splenic bees.
Blank group: the bee space of the group is provided with a bee 40 spleen, and the bee colony does not feed any medicine.
After continuously observing for 15 days, the number of the colonies with the rotten son disease of four groups of bees is recorded, and the statistical data are shown in table 2.
TABLE 2 number of four bee colonies suffering from rotten son's disease
As can be seen from table 2:
a. in the initial stage of the test (test day 2), only one diseased bee colony appears in the blank group, which shows that the traditional Chinese medicine nutrient solution of the example 1, the traditional Chinese medicine composition of the comparative group 1 and the amoxicillin of the comparative group 2 have the effect of preventing the rotten son disease.
b. In the middle period of the test (test day 5 and test day 10), the diseased bee colonies in the blank group are gradually enlarged and difficult to control, and the control group 1 and the control group 2 also generate a small amount of diseased bee colonies and have an increasing trend, which indicates that the traditional Chinese medicine composition in the control group 1 and the amoxicillin in the control group 2 can not effectively inhibit the occurrence of the rotten son disease. In contrast, no diseased bee colonies still appeared in the experimental group, which shows that the nutrient solution of the traditional Chinese medicine in the example 2 can continuously and effectively prevent the rotten son disease.
c. By the end of the test (day 16 of the test), no diseased bee colonies appear in the test group, which shows that the traditional Chinese medicine nutrient solution in the example 2 can continuously and effectively prevent the rotten son diseases. The diseased bee colonies are respectively surged to 14 spleens and 30 spleens by observing the contrast group 1, the contrast group 2 and the blank group, which shows that the prevention and cure of the traditional Chinese medicine composition of the contrast group 1 and the amoxicillin of the contrast group 2 are not good.
In conclusion, the Chinese medicinal nutrient solution can effectively prevent bee rotten son diseases, the medicinal effect is continuous and stable, and the Chinese medicinal nutrient solution adopts natural Chinese medicinal materials, has no medicament residue on bee products, and meets the production safety requirements of pollution-free food.
Experimental example 3
(3) Test for accelerating bee breeding effect
Three healthy swarms are selected to be divided into four groups in the honey lacking season, which are respectively: experiment group, comparison group 1, comparison group 2, blank group, have enough distance between the group, can not advance the wrong case each other.
Experimental groups: the bee colony of the group comprises 5000 bees, and the bee colony is fed with the traditional Chinese medicine nutrient solution of the embodiment 3, and the feeding method comprises the following steps: the Chinese medicine nutrient solution of the embodiment 3 is poured into the bee colony feeder, the amount of each frame of bees is 30g corresponding to the Chinese medicine nutrient solution of the embodiment 3, and the feeding is carried out once every three days and 5 times continuously.
Comparative group 1: the bee field is fed with a pure traditional Chinese medicine composition for treating the sacbrood disease of bees, which is disclosed in Chinese patent CN201210549180. X.
Comparative group 2: 5200 bees exist in the swarm of the group, and the swarm is fed with 1:1 common amoxicillin white sugar water, and the feeding method comprises the following steps: white sugar water is poured into the bee colony feeder for the bees to eat.
Blank group: 5100 bees are in the swarm of the group, and no food or medicine is fed to the swarm.
The number of healthy larvae fed by four groups of bees was recorded after 15 consecutive observations, and the statistical data is shown in table 3.
TABLE 3 healthy larva Nursery by four groups of bees
As can be seen from table 3:
a. in the initial period of using the medicine (test day 3), the hatching number of the larva of the swarms in the experimental group, the comparative group 1 and the comparative group 2 is slightly higher than that of the swarms which are not fed in the blank group.
b. In the middle period of using the medicine (6 th day of test and 9 th day of test), the stimulation advantages of the test group, the control group 1 and the control group 2 are shown, the number of the healthy bee larvae in the test group is increased from 1010 to 2300, and compared with the control group 1, the control group 2 and the blank group, the nutrition supplementing effect is obvious; although the number of the healthy bee larvae of the comparison group 1 and the comparison group 2 is increased slightly, by the 12 th day of the test, the number of the healthy larvae fed by the bees of the comparison group 1 is increased obviously compared with that of the experimental group, the number of the healthy larvae fed by the bees of the comparison group 2 is reduced, which shows that the traditional Chinese medicine composition of the comparison group 1 has poor effect on the healthy larvae fed by the bees, and the amoxicillin white sugar water is used for feeding the bees in the comparison group 2, so that the nutrition of the bees cannot be kept up, the fertility of the bees is insufficient, the bee colony also generates drug resistance, and the number of the healthy larvae is reduced on the contrary; the bees in the blank group are in a broken-son nest-cleaning state because the bees cannot be kept by nutrition.
The data of the experimental group show that from the 3 rd day of the experiment, the number of healthy larvae fed by bees is not continuously reduced along with the external honey deficiency state, but the bee colony is fed actively, and the bee colony is steadily developed and continuously grows.
Experimental example 4
(3) Test of the Effect of mutual mergers between bee colonies
6 healthy queen-free bee colonies were selected and divided into 3 groups, each of which was: experiment group, contrast group, blank group have sufficient distance between group and the group, can not advance wrong case each other, prepare 3 queen bees that will intervene the bee colony again.
Experimental groups: the two swarms of the group have 2000 bees and 2100 bees respectively, and the Chinese medicinal nutrient solution of the embodiment 3 is sprayed to the swarms, and the spraying method comprises the following steps: the traditional Chinese medicine nutrient solution in the embodiment 3 is added with a proper amount of water (the water can be added for spraying), each frame of bees is sprayed until the bees are slightly wet, then two groups of bees are gathered into one group, and then one queen bee is intervened.
Comparison group: the two groups of bees in the group are 2100 and 1900 bees respectively, and the two groups of bees are gathered into a group after the honey wine is sprayed, and then a queen bee is intervened.
Blank group: the two groups of bee colonies of the group are respectively provided with 2200 bees and 2100 bees, and the two groups of bees are directly grouped into a group by any treatment of the bee colonies and then intervened in a queen bee.
The observation was carried out at 1 hour, the number of remaining healthy bees of the three groups of bees was recorded, and the statistical data are shown in table 4.
TABLE 4 number of remaining healthy bees of three groups of bees
As can be seen from table 4:
a. the swarms were pooled after spraying the swarms with the drug (trial hour 2), and the swarms in the experimental and control groups were much higher in the number of healthy bees than the swarms in the blank group without spraying.
b. In the later stage (test hour 4) of the drug-combined bee colony, the advantages of the experimental group and the comparison group are shown, the number of healthy bee larvae in the experimental group is basically not lost, and the queen bee is normal; compared with a comparison group and a blank group, the effects of safe combination and safe intervention on queen bees are remarkable; and the healthy bee larva quantity of contrast group does not have reduction by a wide margin yet, but alcohol has certain stimulus to the honeybee, and honeybee mood is very unstable, and the honeybee still encloses the king after the alcohol volatilizees, bites the king phenomenon and leads to the queen bee wing to be bitten into bad. The bees in the blank group are mutually put on the shelf because the odor does not conform to the standard, and the queen bee is also killed.
The data from the experimental groups showed that from the 1 st hour of the experiment, the number of healthy bees did not decrease continuously, but instead the colony protected the spleen actively, the colony was quiet and stable, and the queen ran normally.
In conclusion, the traditional Chinese medicine nutrient solution for treating bee brood has quick response, stable and continuous drug effect and short curing time, can greatly enhance the feeding capacity of bees, and can be safely and effectively used for mutual combination of bee colonies and intervention of queen loss colonies in queen bees; and the invention adopts a natural Chinese herbal medicine formula, has no medicine residue on bee products, and meets the production safety requirement of pollution-free food.
Claims (8)
1. A traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing feeding capacity is characterized by comprising the following components in parts by weight: 75-85 parts of honey, 1-10 parts of royal jelly and 10-15 parts of a traditional Chinese medicine composition;
the traditional Chinese medicine composition comprises the following components in parts by weight: 30-40 parts of acanthopanax senticosus, 10-30 parts of polygonum cuspidatum, 10-20 parts of folium isatidis, 5-15 parts of scutellaria baicalensis, 5-15 parts of fructus forsythiae, 5-15 parts of sculellaria barbata and 5-15 parts of liquorice.
2. The traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing nursing power of claim 1, which comprises the following components in parts by weight: 80-85 parts of honey, 4-8 parts of royal jelly and 10-15 parts of a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 35-40 parts of acanthopanax senticosus, 15-25 parts of polygonum cuspidatum, 15-20 parts of folium isatidis, 5-10 parts of scutellaria baicalensis, 5-10 parts of fructus forsythiae, 5-10 parts of sculellaria barbata and 10-15 parts of liquorice.
3. The traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing nursing power of claim 1, which comprises the following components in parts by weight: 80 parts of honey, 5 parts of royal jelly and 15 parts of a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 35 parts of acanthopanax senticosus, 20 parts of polygonum cuspidatum, 15 parts of folium isatidis, 10 parts of scutellaria baicalensis, 10 parts of fructus forsythiae, 10 parts of sculellaria barbata and 10 parts of liquorice.
4. The traditional Chinese medicine nutrient solution for preventing and treating bee rotten son disease, unifying group taste and enhancing nursing power of claim 1, which comprises the following components in parts by weight: 80 parts of honey, 10 parts of royal jelly and 10 parts of a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 30 parts of acanthopanax senticosus, 15 parts of polygonum cuspidatum, 20 parts of folium isatidis, 15 parts of scutellaria baicalensis, 5 parts of fructus forsythiae, 10 parts of sculellaria barbata and 10 parts of liquorice.
5. Use of the Chinese medicinal nutrient solution according to any one of claims 1 to 4 in the preparation of a medicament for preventing and treating bee brood, enhancing bee feeding ability, mutual merging between bee colonies, and intervening queen bee colonies.
6. A method for preparing the Chinese medicinal nutrient solution according to any one of claims 1 to 4, comprising the following steps:
1) weighing the components according to the proportion, cleaning, crushing into medium-coarse powder, adding water and decocting for at least 2 times;
2) mixing the liquid medicines obtained after multiple times of decoction, filtering, standing and concentrating to obtain a traditional Chinese medicine preparation;
3) cooling the decocted Chinese medicinal preparation, adding honey and royal jelly, uniformly stirring, and freezing and storing at the temperature of between 15 ℃ below zero and 20 ℃ below zero.
7. The preparation method of the traditional Chinese medicine nutrient solution according to claim 6, wherein in the step 1), the decoction is carried out for three times: a first boiling stage: decocting at 80-100 deg.C for 30-60min, and filtering to obtain medicinal liquid; a second boiling stage: decocting at 60-80 deg.C for 30-60min, and filtering to obtain medicinal liquid; a third boiling stage: decocting at 40-60 deg.C for 10-30min, and filtering to obtain medicinal liquid.
8. The method for preparing the Chinese medicinal nutrient solution according to claim 6, wherein the decocted Chinese medicinal preparation is cooled to below 40 ℃ in the step 3), then the honey and the royal jelly are added and stirred uniformly, and the mixture is frozen and stored at the temperature of between 15 ℃ below zero and 20 ℃ below zero.
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