CN102782122B - 有效建立经诱导的多能干细胞的方法 - Google Patents

有效建立经诱导的多能干细胞的方法 Download PDF

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CN102782122B
CN102782122B CN201180009618.9A CN201180009618A CN102782122B CN 102782122 B CN102782122 B CN 102782122B CN 201180009618 A CN201180009618 A CN 201180009618A CN 102782122 B CN102782122 B CN 102782122B
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CN102782122A (zh
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山中伸弥
五岛直树
前川桃子
河村义史
望月宏美
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JAPAN BIOLOG INFORMATICS CONSO
National Institute of Advanced Industrial Science and Technology AIST
Kyoto University NUC
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Kyoto University NUC
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
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  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201180009618.9A 2010-02-16 2011-02-16 有效建立经诱导的多能干细胞的方法 Active CN102782122B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US30510710P 2010-02-16 2010-02-16
US61/305,107 2010-02-16
US37994910P 2010-09-03 2010-09-03
US61/379,949 2010-09-03
PCT/JP2011/053874 WO2011102531A1 (en) 2010-02-16 2011-02-16 Method of efficiently establishing induced pluripotent stem cells

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CN102782122A CN102782122A (zh) 2012-11-14
CN102782122B true CN102782122B (zh) 2015-02-25

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US (1) US8927277B2 (enExample)
EP (1) EP2536828B1 (enExample)
JP (1) JP5765746B2 (enExample)
KR (1) KR101857302B1 (enExample)
CN (1) CN102782122B (enExample)
CA (1) CA2789749C (enExample)
IL (1) IL221481A (enExample)
SG (1) SG183315A1 (enExample)
WO (1) WO2011102531A1 (enExample)

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CN105861447A (zh) * 2016-06-13 2016-08-17 广州市搏克生物技术有限公司 一种非病毒iPSCs诱导组合物及其试剂盒

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KR101764100B1 (ko) 2009-02-27 2017-08-02 고쿠리츠 다이가쿠 호진 교토 다이가쿠 신규한 핵 재프로그래밍 물질
WO2011016588A1 (en) 2009-08-07 2011-02-10 Kyoto University Method of efficiently establishing induced pluripotent stem cells
US9758765B2 (en) 2011-04-08 2017-09-12 Osaka University Modified laminin and use thereof
WO2012141181A1 (ja) * 2011-04-11 2012-10-18 国立大学法人京都大学 核初期化物質
EP2853592B1 (en) 2012-05-23 2019-02-20 Kyoto University Highly efficient method for establishing artificial pluripotent stem cell
EP2873727B1 (en) * 2012-07-12 2019-11-06 Kyoto Prefectural Public University Corporation Brown fat cells and method for preparing same
SG11201503167XA (en) * 2012-10-23 2015-05-28 Univ Kyoto Method of efficiently establishing induced pluripotent stem cells
AU2014324233B2 (en) * 2013-09-24 2020-07-23 Id Pharma Co., Ltd. Method for improving efficiency of inducing pluripotent stem cell
CN104017777A (zh) * 2014-01-07 2014-09-03 中国人民解放军第三军医大学第一附属医院 用于治疗视网膜疾病的试剂盒
EP3165603B1 (en) * 2014-07-03 2019-09-18 Juntendo Educational Foundation Pancreatic endocrine cells, method for producing same, and transdifferentiation agent
WO2017073740A1 (ja) 2015-10-29 2017-05-04 学校法人埼玉医科大学 膵内分泌細胞の製造方法、及び分化転換剤
US9936734B2 (en) 2016-03-11 2018-04-10 Altria Client Services, Llc. Personal carrying case for electronic vaping device
CN106086071B (zh) * 2016-06-13 2019-08-09 广州市搏克生物技术有限公司 一种非病毒iPSCs诱导方法及其诱导组合物、试剂盒及其获得的iPSCs
US11845960B2 (en) 2016-09-12 2023-12-19 President And Fellows Of Harvard College Transcription factors controlling differentiation of stem cells
CN106939299B (zh) * 2017-02-04 2022-11-15 滨州医学院 microRNA重编程体细胞为神经干细胞的制备方法及应用
WO2018232079A1 (en) 2017-06-14 2018-12-20 Daley George Q Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer
WO2019108894A1 (en) 2017-12-01 2019-06-06 President And Fellows Of Harvard College Methods and compositions for the production of oligodendrocyte progenitor cells
US11788131B2 (en) 2018-04-06 2023-10-17 President And Fellows Of Harvard College Methods of identifying combinations of transcription factors
US20220145255A1 (en) 2019-02-26 2022-05-12 Tohoku University Method for producing osteoblast cluster using ips cells
CN110079459A (zh) * 2019-05-15 2019-08-02 刘宝全 一种干细胞培养容器内气体平衡的调节方法
EP4060023A4 (en) 2019-11-12 2023-12-13 Juntendo Educational Foundation METHOD FOR DIRECT TRANSDIFFERENTIATION OF SOMATIC CELL
MX2022008648A (es) 2020-01-23 2022-12-15 The Children´S Medical Center Corp Diferenciacion de celulas t sin estroma a partir de celulas madre pluripotentes humanas.
CN116426573A (zh) * 2023-04-14 2023-07-14 臻赫医药(杭州)有限公司 重编程因子抗衰老表达系统、生物材料及用途

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105861447A (zh) * 2016-06-13 2016-08-17 广州市搏克生物技术有限公司 一种非病毒iPSCs诱导组合物及其试剂盒
CN105861447B (zh) * 2016-06-13 2017-12-19 广州市搏克生物技术有限公司 一种非病毒iPSCs诱导组合物及其试剂盒

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KR20120131180A (ko) 2012-12-04
KR101857302B1 (ko) 2018-05-11
EP2536828B1 (en) 2015-09-09
JP5765746B2 (ja) 2015-08-19
US20130029423A1 (en) 2013-01-31
WO2011102531A1 (en) 2011-08-25
US8927277B2 (en) 2015-01-06
IL221481A (en) 2016-06-30
CN102782122A (zh) 2012-11-14
CA2789749A1 (en) 2011-08-25
EP2536828A4 (en) 2014-01-15
IL221481A0 (en) 2012-10-31
EP2536828A1 (en) 2012-12-26
SG183315A1 (en) 2012-09-27
CA2789749C (en) 2018-09-04
JP2013519371A (ja) 2013-05-30

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