CN102765863A - Preparation prepared by adopting bacillus amyloliquefaciens for processing excrement in a livestock farm as well as preparation method and processing method thereof - Google Patents
Preparation prepared by adopting bacillus amyloliquefaciens for processing excrement in a livestock farm as well as preparation method and processing method thereof Download PDFInfo
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- CN102765863A CN102765863A CN2012102544858A CN201210254485A CN102765863A CN 102765863 A CN102765863 A CN 102765863A CN 2012102544858 A CN2012102544858 A CN 2012102544858A CN 201210254485 A CN201210254485 A CN 201210254485A CN 102765863 A CN102765863 A CN 102765863A
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Abstract
The invention discloses a preparation prepared by adopting bacillus amyloliquefaciens, as well as a preparation method and a processing method of the preparation. The preparation consists of 85-90 parts of bacillus amyloliquefaciens fermentation liquor, 4-6 parts of a biosurfactant and 2-4 parts of biological preservatives. The preparation method comprises the steps of preparing the bacillus amyloliquefaciens fermentation liquor and blending. The processing method comprises the step that the preparation and organic materials are mixed to ferment. The preparation, the preparation method and the processing method have the advantages that the method is low in cost, reliable in technology, and good in effect, the applicable range is wide, the cleanness level of a pig farm is improved comprehensively, the role of disease control is played, a part of resources can be recycled and utilized, and certain economic returns and promotional value are achieved.
Description
Technical field
The present invention relates to a kind of method of handling feces of farm, the specifically a kind of processing feces of farm preparation of bacillus amyloliquefaciens preparation and method of preparation and processing thereof of adopting belongs to the feces of farm process field.
Background technology
In recent years, the aquaculture of China is cultured from garden rapidly and is developed to intensification, mass-producing, commercialization direction, and particularly around big and medium-sized cities, livestock and poultry farm is more and more, and scale is increasing.The economic benefit that the fowl industry is educated in intensification, mass-producing is very remarkable.But the problem of environmental pollution order benefit of incident aquaculture waste is serious.Feces of farm has only minimum part through harmless processing, and is most unprocessed and directly discharging has caused serious pollution to surface water, underground water and environment.So take measures rapidly, solve the discharging and the problem of environmental pollution of feces of livestock and poultry, become a big problem that is related to rural economic development and 21 century Chinese Ecological Agriculture development.
The excrement of large-scale cultivation field is dirty to be handled, and uses environment protection from reasonable resources, as a kind of available resource, is much accounted of feces of livestock and poultry more and more.Along with the continuous expansion of plant's scale, the dissolving and discharging of ight soil purifies from the primary soil natural, develops into and utilizes various biopurification treatment processs now, to reduce and to stop the livestock and poultry public hazards.In the past the livestock and poultry feces treatment technology great majority of China be conceived to innoxious be devoted to the development of certain single equipment; As adopt the physical treatment process of solid-liquid separation compost, oven dry, firepower or electric drying; The biological treatment of anaerobically fermenting, aerobic aeration, oxidized sludge etc. etc., but effect and not obvious.
Summary of the invention
In order to address the above problem, the present invention has designed a kind of processing feces of farm preparation of bacillus amyloliquefaciens preparation and method of preparation and processing thereof of adopting, and this method cost is low, technology is reliable, effective; Applied widely, improved pig farm sanitation and hygiene level, because the existence of probiotics microorganism solution bacillus amyloliquefaciens comprehensively; And with pathogenic micro-organism competition living environment, make pathogenic micro-organism not have living space and can not breed in a large number, thereby play having an effect of control disease; Also without the chemostefilant sterilization, bedding and padding are piled up after 70 ℃ of fermentations of high temperature at every turn, the ability kill pathogenic bacteria; The disease that mosquitos and flies is propagated also significantly reduces, and improves pig house environment, has effectively reduced the generation of disease and spreads; But also a part of resource of recyclable utilization has certain economic repayment and promotional value.
Technical scheme of the present invention is:
A kind of processing feces of farm preparation that adopts the bacillus amyloliquefaciens preparation, described feces of farm preparation is grouped into by the one-tenth of following weight part:
85～90 parts of bacillus amyloliquefaciens fermented liquids, 4～6 parts of bio-surfactants, 2～4 parts of biological preservatives.
Preferably, 88 parts of bacillus amyloliquefaciens fermented liquids, 5 parts of bio-surfactants, 3 parts of biological preservatives.
Described bacillus amyloliquefaciens; Numbering Strain Number AS1.1099; Latin formal name used at school Latin Name Bacillus amyloliquefaciens (Fukumoto) Priest et al. bacillus amyloliquefaciens is a kind of and the very high bacterium of subtilis affinity; In the process of growth of himself, can produce a series of meta-bolites, these meta-bolitess make bacillus amyloliquefaciens can have the activity that suppresses fungi and bacterium widely.
Further, described bacillus amyloliquefaciens is YN-1 bacterial strain or HN06 bacterial strain.
Described tensio-active agent is an amino acid; Described amino acid is for contain the various aliphatics organic acids of one or more amino and carboxyl simultaneously; Find that through test through can significantly improving the ferment effect of bacillus amyloliquefaciens fermented liquid behind the interpolation amino acid, it is remarkable to handle the dirty effect of excrement.
Described biological preservative is a nisin, also claims that Nutrition 21 or Buddhist nun are hot, is a kind of peptide material that streptococcus acidi lactici produces, and is made up of 34 amino-acid residues.
Above-mentioned processing feces of farm preparation the preparation method, may further comprise the steps:
(1) bacillus amyloliquefaciens test tube kind is inoculated in the liquid nutrient medium; The loading amount volumetric ratio is 2/5; In pH6.5～8, temperature under 25～37 ℃ the condition, the shaking table shaking culture, rotating speed is 120rpm; With the centrifugal 20min of cultured fermented liquid 4000rpm, obtain supernatant and be the bacillus amyloliquefaciens fermented liquid after 2-15 days;
The weight percent of described liquid culture based formulas is: the Carnis Bovis seu Bubali cream of 2-10%, the peptone powder of 1-10%, the K2HP04 of 0.1-3%, the MgS04 of 0.1-1%, the NaCl of 1-5%, water surplus.
Be preferably: 8% Carnis Bovis seu Bubali cream, 8% peptone powder, 2% K2HP04,0.5% MgS04,3% NaCl, water surplus.
(2) the bacillus amyloliquefaciens fermented liquid that above-mentioned steps (1) is obtained mixes with, tensio-active agent and sanitas according to weight ratio and gets final product.
The treatment process of above-mentioned processing feces of farm preparation is: described processing feces of farm preparation is mixed with organic materialss such as husk, sawdusts; Add 1 kilogram of described processing feces of farm preparation in every cubic metre of organic materials; Place mat is in pig house then; Fermented 15 days, and can reach the purpose of deodorizing, purification.
The movement of pig infiltrates in above-mentioned organic bedding and padding; Microbial process through the bacillus amyloliquefaciens fermented liquid; By degraded rapidly, digestion; Utilize the arch of pig to turn over habit and carry out turning over of ight soil and bury, be convenient to new and old bedding and padding renewal and aerobic, anerobes alternately, thereby reach flushing-free swinery, no stink, zero release, realization environmental protection, nuisanceless breed purpose from the source.
Method of the present invention is theoretical according to little ecological theory and biological fermentation; Process mixing organism bedding and padding with compound microorganism ferments bacterium such as husk, sawdust, bacillus amyloliquefaciens and yeast; Place mat is in pig house; The movement of pig infiltrates organic bedding and padding,, is utilized the arch of pig to turn over habit and carries out turning over of ight soil and bury by rapidly degraded, digestion through microbial process; Be convenient to new and old bedding and padding renewal and aerobic, anerobes alternately, thereby reach flushing-free swinery, no stink, zero release, realization environmental protection, nuisanceless breed purpose from the source.In this process, the movement of pig is sneaked in organic bedding and padding, combines with bacillus amyloliquefaciens; Promptly can be made into the fine biological active organic fertilizer through fermentation; The agricultural wastes such as a large amount of straws, straw, wood chip of having dissolved promote the recycle of agricultural resource, can solve the problem of feces of livestock and poultry harmless treatment, crop straw burning again; Improve rural area, agroecological environment, form the ecological agriculture system that people and animal, natural harmony are got along.
The invention has the advantages that:
1, realizes fecaluria zero release truly
Raise pigs and need not wash swinery, the fecaluria of being discharged on pig house is biological fermentation bed through the mikrobe fully fermenting of bacillus amyloliquefaciens fermented liquid and glycolysis rapidly, digestion, thereby realize the zero release of pig manure urine.
2, improve pig farm sanitation and hygiene level comprehensively, improve pig house environment
Pig manure urine by rapid glycolysis digestion, is destitute of smell in the pig house at short notice, does not have fly; Needn't build the blowdown ditch, the mosquito environment of hatching of also not laying eggs, therefore; With fresh air in the pig house; The water and soil of pig farm periphery can be not contaminated, and the whole sanitation and hygiene level in pig farm improves comprehensively, and pig house environment is greatly improved.
3, done efficiency of feed utilization high
Owing to added bacillus amyloliquefaciens fermented liquid biotechnological formulation in the feed, reduced the pH value in the chitling road, strengthen the wriggling and the secretion of enteron aisle, protect digesting and assimilating of feed; Beneficial flora can produce different meta-bolitess and digestive ferment at the growth and breeding of enteron aisle, and these meta-bolitess will decompose the macromolecular substance in the feed, thereby improves the conversion of pig to feed.
4, effectively reduce the generation of disease and spreading
Theoretical according to microbial balance, use the probiotics mikrobe in the surging bacillus amyloliquefaciens fermented liquid to compete living environment with pathogenic micro-organism, make pathogenic micro-organism not have living space and can not breed in a large number, thereby play having an effect of control disease.Without the chemostefilant sterilization, bedding and padding are piled up after the fermentation of 70 ℃ of high temperature at every turn in the hurdle house, the ability kill pathogenic bacteria, and the disease that mosquitos and flies is propagated also significantly reduces.
5, reduce cost consumption such as water power
Culture and adopt free choice feeding, automatic water-drinking, do not need clear excrement, Xi Lan, save a large amount of water power costs without heating facilities winter.
6, reduce the input of fixed capital.
Owing to need not build the dirty treatment facility of excrement such as blowdown ditch, fermentation vat, reduce the input of fixed capital.
7, applied widely
Various breed scales, various region can be suitable for.In the raiser who raises family and various scales scattered, can promote, method is simple.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for explanation and explains the present invention, and be not used in qualification the present invention.
Embodiment 1
A kind of processing feces of farm preparation that adopts the bacillus amyloliquefaciens preparation, described feces of farm preparation is grouped into by the one-tenth of following weight part:
88 parts of bacillus amyloliquefaciens fermented liquids, 5 parts of bio-surfactants, 3 parts of biological preservatives.
Described bacillus amyloliquefaciens is YN-1 bacterial strain or HN06 bacterial strain.
Described tensio-active agent is an amino acid, and described amino acid is for contain the various aliphatics organic acids of one or more amino and carboxyl simultaneously.
Described biological preservative is a nisin, also claims that Nutrition 21 or Buddhist nun are hot, is a kind of peptide material that streptococcus acidi lactici produces, and is made up of 34 amino-acid residues.
Embodiment 2
A kind of processing feces of farm preparation that adopts the bacillus amyloliquefaciens preparation, described feces of farm preparation is grouped into by the one-tenth of following weight part:
85 parts of bacillus amyloliquefaciens fermented liquids, 4 parts of bio-surfactants, 2 parts of biological preservatives.
All the other are identical with embodiment 1.
Embodiment 3
A kind of processing feces of farm preparation that adopts the bacillus amyloliquefaciens preparation, described feces of farm preparation is grouped into by the one-tenth of following weight part:
90 parts of bacillus amyloliquefaciens fermented liquids, 6 parts of bio-surfactants, 4 parts of biological preservatives.
All the other are identical with embodiment 1.
Embodiment 4
The said processing feces of farm of the foregoing description 1-3 preparation the preparation method, may further comprise the steps:
(1) bacillus amyloliquefaciens test tube kind is inoculated in the liquid nutrient medium; The loading amount volumetric ratio is 2/5; In pH6.5～8, temperature under 25～37 ℃ the condition, the shaking table shaking culture, rotating speed is 120rpm; With the centrifugal 20min of cultured fermented liquid 4000rpm, obtain supernatant and be the bacillus amyloliquefaciens fermented liquid after 2-15 days;
Described liquid culture based formulas is: the Carnis Bovis seu Bubali cream of 2-10%, the peptone powder of 1-10%, the K2HP04 of 0.1-3%, the MgS04 of 0.1-1%, the NaCl of 1-5%, water surplus.
(2) the bacillus amyloliquefaciens fermented liquid that above-mentioned steps (1) is obtained mixes with, tensio-active agent and sanitas according to weight ratio and gets final product.
Embodiment 5
The treatment process of above-mentioned processing feces of farm preparation is: described processing feces of farm preparation is mixed with organic materialss such as husk, sawdusts; Add 1 kilogram of described processing feces of farm preparation in every cubic metre of organic materials; Place mat is in pig house then; Fermented 15 days, and can reach the purpose of deodorizing, purification.
Bacillus amyloliquefaciens described in the foregoing description is YN-1 bacterial strain or HN06 bacterial strain, is provided by national yield increasing fungus technical study and popularization center.
Except as otherwise noted, the percentage ratio that is adopted among the present invention is weight percentage.
What should explain at last is: the above is merely the preferred embodiments of the present invention; Be not limited to the present invention; Although the present invention has been carried out detailed explanation with reference to previous embodiment; For a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment put down in writing, and perhaps part technical characterictic wherein is equal to replacement.All within spirit of the present invention and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. processing feces of farm preparation that adopts bacillus amyloliquefaciens preparation is characterized in that described feces of farm preparation is grouped into by the one-tenth of following weight part:
85～90 parts of bacillus amyloliquefaciens fermented liquids, 4～6 parts of bio-surfactants, 2～4 parts of biological preservatives.
2. preparation according to claim 1 is characterized in that: described feces of farm preparation is grouped into by the one-tenth of following weight part:
88 parts of bacillus amyloliquefaciens fermented liquids, 5 parts of bio-surfactants, 3 parts of biological preservatives.
3. preparation according to claim 1 is characterized in that: described bacillus amyloliquefaciens is YN-1 bacterial strain or HN06 bacterial strain.
4. preparation according to claim 1 is characterized in that: described tensio-active agent is an amino acid.
5. preparation according to claim 1 is characterized in that: described biological preservative is a nisin.
A processing feces of farm preparation as claimed in claim 1 the preparation method, it is characterized in that, may further comprise the steps:
(1) bacillus amyloliquefaciens test tube kind is inoculated in the liquid nutrient medium; The loading amount volumetric ratio is 2/5; In pH6.5～8, temperature under 25～37 ℃ the condition, the shaking table shaking culture, rotating speed is 120rpm; With the centrifugal 20min of cultured fermented liquid 4000rpm, obtain supernatant and be the bacillus amyloliquefaciens fermented liquid after 2-15 days;
(2) the bacillus amyloliquefaciens fermented liquid that above-mentioned steps (1) is obtained mixes with tensio-active agent and sanitas according to weight ratio and gets final product.
7. preparation method according to claim 6 is characterized in that: described tensio-active agent is an amino acid.
8. preparation method according to claim 6 is characterized in that: described biological preservative is a nisin.
9. preparation method according to claim 6 is characterized in that, the weight percent of described liquid culture based formulas is: the Carnis Bovis seu Bubali cream of 2-10%; The peptone powder of 1-10%, the K2HP04 of 0.1-3%, the MgS04 of 0.1-1%; The NaCl of 1-5%, water surplus.
10. the treatment process of a processing feces of farm preparation as claimed in claim 1; It is characterized in that: described processing feces of farm preparation is mixed with husk and sawdust organic materials; Add 1 kilogram of described processing feces of farm preparation in every cubic metre of described organic materials; Place mat fermented 15 days in pig house then, got final product.
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| CN104355500A (en) * | 2014-11-18 | 2015-02-18 | 华中农业大学 | Piggery wastewater treatment and recycling method |
| CN107601680A (en) * | 2017-10-24 | 2018-01-19 | 临沂市畜牧站 | The dirty method of biofermentation harmless treatment excrement |
| CN110669811A (en) * | 2019-10-21 | 2020-01-10 | 天津大学 | Method for improving surfactant yield |
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Cited By (4)
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| CN110669811A (en) * | 2019-10-21 | 2020-01-10 | 天津大学 | Method for improving surfactant yield |
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Application publication date: 20121107 |