CN102753707A - 用随机引物和特异性引物的混合物等温扩增核酸 - Google Patents

用随机引物和特异性引物的混合物等温扩增核酸 Download PDF

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Publication number
CN102753707A
CN102753707A CN2011800087156A CN201180008715A CN102753707A CN 102753707 A CN102753707 A CN 102753707A CN 2011800087156 A CN2011800087156 A CN 2011800087156A CN 201180008715 A CN201180008715 A CN 201180008715A CN 102753707 A CN102753707 A CN 102753707A
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nucleic acid
amplification
dna
primer
primers
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Chinese (zh)
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J.R.奈尔逊
W.高
M.赵
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General Electric Co
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General Electric Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CN2011800087156A 2010-02-09 2011-02-08 用随机引物和特异性引物的混合物等温扩增核酸 Pending CN102753707A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/702,884 US20110195457A1 (en) 2010-02-09 2010-02-09 Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
US12/702,884 2010-02-09
PCT/US2011/023996 WO2011142861A2 (en) 2010-02-09 2011-02-08 Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof

Publications (1)

Publication Number Publication Date
CN102753707A true CN102753707A (zh) 2012-10-24

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CN2011800087156A Pending CN102753707A (zh) 2010-02-09 2011-02-08 用随机引物和特异性引物的混合物等温扩增核酸

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Country Link
US (1) US20110195457A1 (cg-RX-API-DMAC7.html)
EP (1) EP2534268A4 (cg-RX-API-DMAC7.html)
JP (1) JP2013518598A (cg-RX-API-DMAC7.html)
CN (1) CN102753707A (cg-RX-API-DMAC7.html)
AU (1) AU2011253427B2 (cg-RX-API-DMAC7.html)
BR (1) BR112012019396A2 (cg-RX-API-DMAC7.html)
CA (1) CA2788821A1 (cg-RX-API-DMAC7.html)
IN (1) IN2012DN06299A (cg-RX-API-DMAC7.html)
WO (1) WO2011142861A2 (cg-RX-API-DMAC7.html)

Cited By (5)

* Cited by examiner, † Cited by third party
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CN105992815A (zh) * 2013-10-28 2016-10-05 韩国科学技术研究院 多孔结构及其制造方法
CN106460040A (zh) * 2014-03-26 2017-02-22 通用电气公司 在低盐条件下的等温扩增
CN108350492A (zh) * 2015-11-05 2018-07-31 通用电气公司 连接酶辅助的核酸环化和扩增
CN110195056A (zh) * 2019-05-31 2019-09-03 汪雪 一种双引物及其应用
CN111334562A (zh) * 2018-12-19 2020-06-26 合肥铼科生物科技有限公司 一种含有修饰引物的核酸扩增方法和试剂盒

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JP2012050393A (ja) * 2010-09-02 2012-03-15 Sony Corp 核酸等温増幅方法
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
US9353393B2 (en) * 2012-02-15 2016-05-31 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
EP2877593B1 (en) * 2012-07-26 2018-07-18 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
JP6366896B2 (ja) * 2013-03-07 2018-08-01 国立研究開発法人農業・食品産業技術総合研究機構 Ｄｎａ親和性物質含有組成物、ｄｎａ親和性物質の精製方法、ｄｎａ親和性物質含有組成物の製造方法およびｄｎａ親和性物質を精製するためのキット
US9422535B2 (en) 2013-04-25 2016-08-23 Thermo Fisher Scientific Baltics Uab phi29 DNA polymerase mutants having increased thermostability and processivity
US9644232B2 (en) * 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9938568B2 (en) 2013-07-26 2018-04-10 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9410172B2 (en) * 2013-09-16 2016-08-09 General Electric Company Isothermal amplification using oligocation-conjugated primer sequences
WO2015084802A1 (en) * 2013-12-02 2015-06-11 Regents Of The University Of Minnesota Rna amplification and oligonucleotide library preparation
JP6666268B2 (ja) 2014-06-11 2020-03-13 サンプリクス アンパーツゼルスカブ 液滴ソートによるヌクレオチド配列排除富化（ｎｅｅｄｌｓ）
WO2016170179A1 (en) * 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
US11085073B2 (en) 2015-04-24 2021-08-10 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
JP2018525004A (ja) * 2015-08-17 2018-09-06 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 微小液滴ベースの多置換増幅（ｍｄａ）方法及び関連組成物
JP7056012B2 (ja) * 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたｄｎａライブラリーの作製方法
CN110506114B (zh) * 2017-06-20 2023-12-15 深圳华大智造科技股份有限公司 Pcr引物对及其应用
JP7748093B2 (ja) * 2021-11-10 2025-10-02 国立大学法人広島大学 鋳型ｄｎａの製造方法
DE102022211087A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches

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US20030207267A1 (en) * 2000-06-28 2003-11-06 Lasken Roger S. Multiply-primed amplification of nucleic acid sequences
US20040209298A1 (en) * 2003-03-07 2004-10-21 Emmanuel Kamberov Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
EP1482059A1 (en) * 2003-05-28 2004-12-01 Agilent Technologies, Inc. Comparative genomic hybridization assays using immobilized oligonucleotide targets
US20040248105A1 (en) * 2003-06-06 2004-12-09 Gyanendra Kumar Method of whole genome amplification with reduced artifact production
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WO2008118679A1 (en) * 2007-03-23 2008-10-02 Ge Healthcare Bio-Sciences Corp. Multiply-primed amplification of circular nucleic acid sequences
US20090035279A1 (en) * 2007-06-13 2009-02-05 Decode Genetics Efh Genetic variants on chr 15q24 as markers for use in diagnosis, prognosis and treatment of exfoliation syndrome and glaucoma
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US20100022412A1 (en) * 2008-07-02 2010-01-28 Roberto Rigatti Using populations of beads for the fabrication of arrays on surfaces

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US20030207267A1 (en) * 2000-06-28 2003-11-06 Lasken Roger S. Multiply-primed amplification of nucleic acid sequences
US20080128298A1 (en) * 2001-10-15 2008-06-05 Carole Bornarth Nucleic acid amplification
US20040209298A1 (en) * 2003-03-07 2004-10-21 Emmanuel Kamberov Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
EP1482059A1 (en) * 2003-05-28 2004-12-01 Agilent Technologies, Inc. Comparative genomic hybridization assays using immobilized oligonucleotide targets
US20040248105A1 (en) * 2003-06-06 2004-12-09 Gyanendra Kumar Method of whole genome amplification with reduced artifact production
WO2008118679A1 (en) * 2007-03-23 2008-10-02 Ge Healthcare Bio-Sciences Corp. Multiply-primed amplification of circular nucleic acid sequences
US20090035279A1 (en) * 2007-06-13 2009-02-05 Decode Genetics Efh Genetic variants on chr 15q24 as markers for use in diagnosis, prognosis and treatment of exfoliation syndrome and glaucoma
US20090203085A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Isothermal Nucleic Acid Amplification Methods and Compositions
US20100022412A1 (en) * 2008-07-02 2010-01-28 Roberto Rigatti Using populations of beads for the fabrication of arrays on surfaces

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105992815A (zh) * 2013-10-28 2016-10-05 韩国科学技术研究院 多孔结构及其制造方法
CN106460040A (zh) * 2014-03-26 2017-02-22 通用电气公司 在低盐条件下的等温扩增
CN106460040B (zh) * 2014-03-26 2021-01-08 通用电气公司 在低盐条件下的等温扩增
CN108350492A (zh) * 2015-11-05 2018-07-31 通用电气公司 连接酶辅助的核酸环化和扩增
CN111334562A (zh) * 2018-12-19 2020-06-26 合肥铼科生物科技有限公司 一种含有修饰引物的核酸扩增方法和试剂盒
CN110195056A (zh) * 2019-05-31 2019-09-03 汪雪 一种双引物及其应用

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Publication number Publication date
WO2011142861A9 (en) 2012-03-15
IN2012DN06299A (cg-RX-API-DMAC7.html) 2015-09-25
EP2534268A2 (en) 2012-12-19
WO2011142861A3 (en) 2012-02-02
JP2013518598A (ja) 2013-05-23
BR112012019396A2 (pt) 2017-06-06
CA2788821A1 (en) 2011-11-17
WO2011142861A2 (en) 2011-11-17
EP2534268A4 (en) 2013-09-04
AU2011253427A1 (en) 2012-08-23
US20110195457A1 (en) 2011-08-11
AU2011253427B2 (en) 2015-06-25

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Application publication date: 20121024