CN102630140A - 用于转化栅藻和杜氏藻叶绿体基因组的系统 - Google Patents

用于转化栅藻和杜氏藻叶绿体基因组的系统 Download PDF

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CN102630140A
CN102630140A CN2010800525438A CN201080052543A CN102630140A CN 102630140 A CN102630140 A CN 102630140A CN 2010800525438 A CN2010800525438 A CN 2010800525438A CN 201080052543 A CN201080052543 A CN 201080052543A CN 102630140 A CN102630140 A CN 102630140A
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nucleotide sequence
grid algae
algae
sequence
seq
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K·博施
S·塞卡
W·莱文
A·卡兰
B·奥奈尔
M·门德斯
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Sapphire Energy Inc
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source

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CN2010800525438A 2009-09-15 2010-09-14 用于转化栅藻和杜氏藻叶绿体基因组的系统 Pending CN102630140A (zh)

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US24273509P 2009-09-15 2009-09-15
US61/242735 2009-09-15
PCT/US2010/048828 WO2011034863A1 (en) 2009-09-15 2010-09-14 A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.

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US (1) US20120252054A1 (enExample)
EP (1) EP2477478B1 (enExample)
CN (1) CN102630140A (enExample)
AU (1) AU2010295739B2 (enExample)
CA (1) CA2773675A1 (enExample)
IN (1) IN2012DN01694A (enExample)
SG (1) SG179157A1 (enExample)
WO (1) WO2011034863A1 (enExample)

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CN107988129A (zh) * 2017-11-27 2018-05-04 浙江大学 改良螺旋藻关键酶基因提高生长固碳速率的方法
CN110747225A (zh) * 2019-12-24 2020-02-04 中国科学院烟台海岸带研究所 一种斜生栅藻叶绿体同源重组空载体及其应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法

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US9273298B2 (en) 2010-10-27 2016-03-01 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene
CA2822467A1 (en) 2010-12-22 2012-06-28 Danisco Us Inc. Compositions and methods for improved isoprene production using two types of ispg enzymes
US8691541B2 (en) 2010-12-22 2014-04-08 Danisco Us Inc. Biological production of pentose sugars using recombinant cells
US9175256B2 (en) 2010-12-23 2015-11-03 Exxonmobil Research And Engineering Company Production of fatty acids and fatty acid derivatives by recombinant microorganisms expressing polypeptides having lipolytic activity
AU2012249389A1 (en) 2011-04-29 2013-11-14 Danisco Us Inc. Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, HMG-CoA synthase and HMG-CoA reductase enzymatic activities
ES2699747T3 (es) 2011-04-29 2019-02-12 Danisco Us Inc Microorganismos recombinantes para la producción potenciada de mevalonato, isopreno e isoprenoides
CA2844064A1 (en) 2011-08-04 2013-02-07 Danisco Us Inc. Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase
US8993305B2 (en) 2011-10-07 2015-03-31 Danisco Us Inc. Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
US8975051B2 (en) 2011-12-23 2015-03-10 Danisco Us Inc. Enhanced production of isoprene using host cells having decreased ispA activity
WO2013096917A1 (en) 2011-12-23 2013-06-27 Danisco Us Inc. Production of isoprene under reduced oxygen inlet levels
US9315831B2 (en) 2012-03-30 2016-04-19 Danisco Us Inc. Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids
US8895277B2 (en) 2012-05-02 2014-11-25 Danisco Us Inc. Legume isoprene synthase for production of isoprene
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
US9328336B2 (en) 2012-10-16 2016-05-03 Exxonmobil Research And Engineering Company DGAT genes and methods of use for triglyceride production in recombinant microorganisms
MX2015006958A (es) * 2012-12-03 2016-02-05 Adi Zaltsman Autofijacion de nitrogeno en plantas imitando las vias procarioticas.
CA2893692C (en) 2012-12-04 2019-06-25 Exxonmobil Research And Engineering Company Tetraselmis promoters and terminators for use in eukaryotic cells
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WO2014088583A1 (en) 2012-12-06 2014-06-12 Exxonmobil Research And Engineering Company Dgat genes comprising pleckstrin homology domains and use in recombinant microorganisms
JP2016511630A (ja) 2012-12-21 2016-04-21 ダニスコ・ユーエス・インク 代案の下流メバロン酸経路を使用するイソプレン、イソプレノイド、およびイソプレノイド前駆体の生成
CN105555952A (zh) 2013-04-10 2016-05-04 丹尼斯科美国公司 用于乙酰辅酶a-衍生代谢物、异戊二烯、类异戊二烯前体和类异戊二烯的改善生产的磷酸解酮酶
DK3107993T3 (da) 2014-02-20 2020-11-23 Danisco Us Inc Rekombinante mikroorganismer til forbedret fremstilling af mevalonat, isopren, isoprenoide forløbere, isoprenoider og acetyl-coa-afledte produkter
FR3041351B1 (fr) * 2015-09-17 2020-01-24 Eranova Procede de preparation d'une poudre d'algues a teneur reduite en proteines et composition bioplastique formulee a partir d'une telle poudre
WO2018098001A1 (en) 2016-11-27 2018-05-31 Triton Algae Innovations, Inc. Method of purification of recombinant osteopontin from microalgae
KR20190120238A (ko) 2017-02-06 2019-10-23 지머젠 인코포레이티드 발효에 의한 티라민의 제조를 위해 조작된 생합성 경로
CN106755537B (zh) * 2017-03-02 2021-04-02 云南纳博生物科技有限公司 一种准确检测叶绿体转化同质化程度的方法
WO2019036140A1 (en) 2017-07-17 2019-02-21 Zymergen Inc. METALLO-ORGANIC STRESS MATERIALS
JP2020536993A (ja) 2017-10-05 2020-12-17 ザイマージェン インコーポレイテッド 光学的に透明なポリイミド
KR20200095534A (ko) 2017-12-07 2020-08-10 지머젠 인코포레이티드 발효에 의한 (6e)-8-히드록시제라니올의 제조를 위해 조작된 생합성 경로
KR20200111172A (ko) 2017-12-21 2020-09-28 지머젠 인코포레이티드 네페탈락톨 산화 환원 효소, 네페탈락톨 합성 효소, 및 네페탈락톤을 생산할 수 있는 미생물
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107988129A (zh) * 2017-11-27 2018-05-04 浙江大学 改良螺旋藻关键酶基因提高生长固碳速率的方法
CN107988129B (zh) * 2017-11-27 2020-07-14 浙江大学 改良螺旋藻关键酶基因提高生长固碳速率的方法
CN110747225A (zh) * 2019-12-24 2020-02-04 中国科学院烟台海岸带研究所 一种斜生栅藻叶绿体同源重组空载体及其应用
CN110747225B (zh) * 2019-12-24 2020-04-28 中国科学院烟台海岸带研究所 一种斜生栅藻叶绿体同源重组空载体及其应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
WO2024026923A1 (zh) * 2022-08-01 2024-02-08 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法

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AU2010295739B2 (en) 2016-02-25
SG179157A1 (en) 2012-05-30
EP2477478A1 (en) 2012-07-25
EP2477478A4 (en) 2013-02-13
IN2012DN01694A (enExample) 2015-06-05
CA2773675A1 (en) 2011-03-24
AU2010295739A1 (en) 2012-03-08
WO2011034863A1 (en) 2011-03-24
EP2477478B1 (en) 2016-11-30
US20120252054A1 (en) 2012-10-04

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Application publication date: 20120808