CN102630140A - 用于转化栅藻和杜氏藻叶绿体基因组的系统 - Google Patents

用于转化栅藻和杜氏藻叶绿体基因组的系统 Download PDF

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CN102630140A
CN102630140A CN2010800525438A CN201080052543A CN102630140A CN 102630140 A CN102630140 A CN 102630140A CN 2010800525438 A CN2010800525438 A CN 2010800525438A CN 201080052543 A CN201080052543 A CN 201080052543A CN 102630140 A CN102630140 A CN 102630140A
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nucleotide sequence
grid algae
algae
sequence
seq
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K·博施
S·塞卡
W·莱文
A·卡兰
B·奥奈尔
M·门德斯
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Sapphire Energy Inc
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source

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CN2010800525438A 2009-09-15 2010-09-14 用于转化栅藻和杜氏藻叶绿体基因组的系统 Pending CN102630140A (zh)

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US24273509P 2009-09-15 2009-09-15
US61/242735 2009-09-15
PCT/US2010/048828 WO2011034863A1 (en) 2009-09-15 2010-09-14 A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.

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CN102630140A true CN102630140A (zh) 2012-08-08

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US (1) US20120252054A1 (enExample)
EP (1) EP2477478B1 (enExample)
CN (1) CN102630140A (enExample)
AU (1) AU2010295739B2 (enExample)
CA (1) CA2773675A1 (enExample)
IN (1) IN2012DN01694A (enExample)
SG (1) SG179157A1 (enExample)
WO (1) WO2011034863A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107988129A (zh) * 2017-11-27 2018-05-04 浙江大学 改良螺旋藻关键酶基因提高生长固碳速率的方法
CN110747225A (zh) * 2019-12-24 2020-02-04 中国科学院烟台海岸带研究所 一种斜生栅藻叶绿体同源重组空载体及其应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI427149B (zh) 2009-06-17 2014-02-21 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
BR112013010551A8 (pt) 2010-10-27 2018-07-03 Danisco Us Inc variantes de isopreno sintase para produção aprimorada de isopreno
CN103476926A (zh) 2010-12-22 2013-12-25 丹尼斯科美国公司 使用两种类型的ispg酶改善异戊二烯产量的组合物和方法
US8691541B2 (en) 2010-12-22 2014-04-08 Danisco Us Inc. Biological production of pentose sugars using recombinant cells
WO2012087676A2 (en) 2010-12-23 2012-06-28 Exxonmobil Research And Enginnering Company Production of fatty acids and fatty acid derivatives by recombinant microorganisms expressing polypeptides having lipolytic activity
BR112013027555A2 (pt) 2011-04-29 2021-08-10 Danisco Us Inc. células recombinantes capazes de maior produção de isopreno, mevalonato e isoprenoides e método para produzir isopreno, mevalonato e isoprenoides
EP2702139A1 (en) 2011-04-29 2014-03-05 Danisco US Inc. Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities
SG2014007991A (en) 2011-08-04 2014-03-28 Danisco Us Inc Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase
JP6420147B2 (ja) 2011-10-07 2018-11-07 ダニスコ・ユーエス・インク メバロン酸、イソプレノイド前駆体、及びイソプレンの産生におけるホスホケトラーゼの使用
JP2015510392A (ja) 2011-12-23 2015-04-09 ダニスコ・ユーエス・インク 低減したｉｓｐａ活性を有する宿主細胞を用いるイソプレン産生増強法
WO2013096917A1 (en) 2011-12-23 2013-06-27 Danisco Us Inc. Production of isoprene under reduced oxygen inlet levels
US9315831B2 (en) 2012-03-30 2016-04-19 Danisco Us Inc. Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
US8895277B2 (en) 2012-05-02 2014-11-25 Danisco Us Inc. Legume isoprene synthase for production of isoprene
US9328336B2 (en) 2012-10-16 2016-05-03 Exxonmobil Research And Engineering Company DGAT genes and methods of use for triglyceride production in recombinant microorganisms
US20140196178A1 (en) * 2012-12-03 2014-07-10 Adi Zaltsman Plant self nitrogen fixation by mimicking prokaryotic pathways
CA2893692C (en) 2012-12-04 2019-06-25 Exxonmobil Research And Engineering Company Tetraselmis promoters and terminators for use in eukaryotic cells
WO2014088563A1 (en) 2012-12-05 2014-06-12 Exxonmobil Research And Engineering Company Nannochloropsis kozak consensus sequence
CA2893926A1 (en) 2012-12-06 2014-06-12 Exxonmobil Research And Engineering Company Dgat genes comprising pleckstrin homology domains and use in recombinant microorganisms
EP2935364A2 (en) 2012-12-21 2015-10-28 Danisco US Inc. Production of isoprene, isoprenoid, and isoprenoid precursors using an alternative lower mevalonate pathway
WO2014169144A2 (en) 2013-04-10 2014-10-16 Danisco Us Inc. Phosphoketolases foe improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precurosors, and isoprenoids
DK3107993T3 (da) 2014-02-20 2020-11-23 Danisco Us Inc Rekombinante mikroorganismer til forbedret fremstilling af mevalonat, isopren, isoprenoide forløbere, isoprenoider og acetyl-coa-afledte produkter
FR3041351B1 (fr) * 2015-09-17 2020-01-24 Eranova Procede de preparation d'une poudre d'algues a teneur reduite en proteines et composition bioplastique formulee a partir d'une telle poudre
WO2018098001A1 (en) 2016-11-27 2018-05-31 Triton Algae Innovations, Inc. Method of purification of recombinant osteopontin from microalgae
CN110494560A (zh) 2017-02-06 2019-11-22 齐默尔根公司 通过发酵产生酪胺的经改造的生物合成途径
CN106755537B (zh) * 2017-03-02 2021-04-02 云南纳博生物科技有限公司 一种准确检测叶绿体转化同质化程度的方法
WO2019036140A1 (en) 2017-07-17 2019-02-21 Zymergen Inc. METALLO-ORGANIC STRESS MATERIALS
JP2020536993A (ja) 2017-10-05 2020-12-17 ザイマージェン インコーポレイテッド 光学的に透明なポリイミド
CN111699247A (zh) 2017-12-07 2020-09-22 齐默尔根公司 通过发酵产生(6e)-8-羟基香叶醇的经改造的生物合成途径
BR112020012704A8 (pt) 2017-12-21 2022-03-03 Zymergen Inc Nepetalactol oxidorredutases, nepetalactol sintases, e micróbios capazes de produzir nepetalactona
US11718857B2 (en) * 2019-08-09 2023-08-08 Alliance For Sustainable Energy, Llc Broad host range genetic tools for engineering microalgae
EP4061936A1 (en) 2019-11-20 2022-09-28 Corbion Biotech, Inc. Sucrose invertase variants
CN115820780A (zh) * 2022-11-24 2023-03-21 云南爱尔发生物技术股份有限公司 一种利用褪黑素联合乙烯利促进栅藻积累叶黄素的方法
CN118475621A (zh) 2023-06-19 2024-08-09 杭州恩和生物科技有限公司 生产7-脱氢胆固醇的融合多肽及其使用方法
CN118691958B (zh) * 2024-06-11 2025-01-10 广东锐创生态科技有限公司 一种基于机器视觉的水产区域监控分析方法及相关装置

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007133558A2 (en) * 2006-05-09 2007-11-22 The Scripps Research Institute Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast
WO2008124526A1 (en) * 2007-04-03 2008-10-16 Saphire Andrew C S Methods for transforming eukaryotic algae
WO2009036385A2 (en) * 2007-09-12 2009-03-19 Kuehnle Agrosystems, Inc. Expression of nucleic acid sequences for production of biofuels and other products in algae and cyanobacteria
WO2009036067A2 (en) * 2007-09-11 2009-03-19 Sapphire Energy Molecule production by photosynthetic organisms
CN101479378A (zh) * 2006-06-29 2009-07-08 加利福尼亚大学董事会 使用经遗传工程改造的微观藻类、蓝细菌或细菌生产短链挥发烃类

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070065900A1 (en) * 2005-09-19 2007-03-22 Dicosimo Deana J Process for chromosomal expression of foreign genes in the cytochrome-C peroxidase (cytCP) region of a methylotrophic microbial host cell
US7947478B2 (en) * 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
GB2460351B (en) * 2007-10-05 2011-03-30 Sapphire Energy Inc System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts
WO2010051489A1 (en) * 2008-10-31 2010-05-06 Sapphire Energy, Inc. Animal feedstock comprising genetically modified algae
AU2009333021B2 (en) * 2008-12-31 2015-09-10 Sapphire Energy, Inc. Genetically engineered herbicide resistant algae
WO2010104763A1 (en) * 2009-03-11 2010-09-16 Sapphire Energy, Inc. Biofuel production in prokaryotes and eukaryotes
US8987433B2 (en) * 2009-03-27 2015-03-24 Sapphire Energy, Inc. Variant isoprenoid producing enzymes and uses thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007133558A2 (en) * 2006-05-09 2007-11-22 The Scripps Research Institute Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast
CN101479378A (zh) * 2006-06-29 2009-07-08 加利福尼亚大学董事会 使用经遗传工程改造的微观藻类、蓝细菌或细菌生产短链挥发烃类
WO2008124526A1 (en) * 2007-04-03 2008-10-16 Saphire Andrew C S Methods for transforming eukaryotic algae
WO2009036067A2 (en) * 2007-09-11 2009-03-19 Sapphire Energy Molecule production by photosynthetic organisms
WO2009036385A2 (en) * 2007-09-12 2009-03-19 Kuehnle Agrosystems, Inc. Expression of nucleic acid sequences for production of biofuels and other products in algae and cyanobacteria

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CONGPING TAN等: ""Establishment of a Micro-Particle Bombardment Transformation System for Dunaliella salina"", 《THE JOURNAL OF MICROBIOLOGY》 *
潘卫东等: ""杜氏盐藻叶绿体转化载体pDS16S-CAT的构建"", 《郑州大学学报（医学版）》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107988129A (zh) * 2017-11-27 2018-05-04 浙江大学 改良螺旋藻关键酶基因提高生长固碳速率的方法
CN107988129B (zh) * 2017-11-27 2020-07-14 浙江大学 改良螺旋藻关键酶基因提高生长固碳速率的方法
CN110747225A (zh) * 2019-12-24 2020-02-04 中国科学院烟台海岸带研究所 一种斜生栅藻叶绿体同源重组空载体及其应用
CN110747225B (zh) * 2019-12-24 2020-04-28 中国科学院烟台海岸带研究所 一种斜生栅藻叶绿体同源重组空载体及其应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
WO2024026923A1 (zh) * 2022-08-01 2024-02-08 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法

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WO2011034863A1 (en) 2011-03-24
SG179157A1 (en) 2012-05-30
AU2010295739A1 (en) 2012-03-08
EP2477478A1 (en) 2012-07-25
EP2477478A4 (en) 2013-02-13
CA2773675A1 (en) 2011-03-24
EP2477478B1 (en) 2016-11-30
IN2012DN01694A (enExample) 2015-06-05
US20120252054A1 (en) 2012-10-04

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Application publication date: 20120808