CA2773675A1 - A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. - Google Patents
A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. Download PDFInfo
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- CA2773675A1 CA2773675A1 CA2773675A CA2773675A CA2773675A1 CA 2773675 A1 CA2773675 A1 CA 2773675A1 CA 2773675 A CA2773675 A CA 2773675A CA 2773675 A CA2773675 A CA 2773675A CA 2773675 A1 CA2773675 A1 CA 2773675A1
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- scenedesmus
- nucleic acid
- nucleotide sequence
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- isolated
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- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C12N9/242—Fungal source
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| PCT/US2010/048828 WO2011034863A1 (en) | 2009-09-15 | 2010-09-14 | A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
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| CN115820780A (zh) * | 2022-11-24 | 2023-03-21 | 云南爱尔发生物技术股份有限公司 | 一种利用褪黑素联合乙烯利促进栅藻积累叶黄素的方法 |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201412988A (zh) | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造 |
| CA2816306A1 (en) | 2010-10-27 | 2012-05-03 | Danisco Us Inc. | Isoprene synthase variants for improved production of isoprene |
| WO2012088450A1 (en) | 2010-12-22 | 2012-06-28 | Danisco Us Inc. | Biological production of pentose sugars using recombinant cells |
| SG191235A1 (en) | 2010-12-22 | 2013-07-31 | Danisco Us Inc | Compositions and methods for improved isoprene production using two types of ispg enzymes |
| US9175256B2 (en) | 2010-12-23 | 2015-11-03 | Exxonmobil Research And Engineering Company | Production of fatty acids and fatty acid derivatives by recombinant microorganisms expressing polypeptides having lipolytic activity |
| CA2834379A1 (en) | 2011-04-29 | 2012-11-01 | The Goodyear Tire & Rubber Company | Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities |
| ES2699747T3 (es) | 2011-04-29 | 2019-02-12 | Danisco Us Inc | Microorganismos recombinantes para la producción potenciada de mevalonato, isopreno e isoprenoides |
| EP2739658A1 (en) | 2011-08-04 | 2014-06-11 | Danisco US Inc. | Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase |
| EP3339426B1 (en) | 2011-10-07 | 2022-08-24 | Danisco US Inc. | Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene |
| US8865442B2 (en) | 2011-12-23 | 2014-10-21 | Danisco Us Inc. | Production of isoprene under reduced oxygen inlet levels |
| SG11201403163QA (en) | 2011-12-23 | 2014-09-26 | Danisco Us Inc | Enhanced production of isoprene using host cells having decreased ispa activity |
| US9315831B2 (en) | 2012-03-30 | 2016-04-19 | Danisco Us Inc. | Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids |
| US9163263B2 (en) | 2012-05-02 | 2015-10-20 | The Goodyear Tire & Rubber Company | Identification of isoprene synthase variants with improved properties for the production of isoprene |
| US8895277B2 (en) | 2012-05-02 | 2014-11-25 | Danisco Us Inc. | Legume isoprene synthase for production of isoprene |
| EP2909304A4 (en) | 2012-10-16 | 2016-09-21 | Exxonmobil Res & Eng Co | DGAT GENE AND METHOD FOR USE IN TRIGLYCERIDE PRODUCTION IN RECOMBINANT MICROORGANISMS |
| BR112015013058A2 (pt) * | 2012-12-03 | 2017-09-12 | Zaltsman Adi | autofixação de nitrogênio em plantas por mimetização de vias procarióticas |
| CA2893692C (en) | 2012-12-04 | 2019-06-25 | Exxonmobil Research And Engineering Company | Tetraselmis promoters and terminators for use in eukaryotic cells |
| EP2931739A4 (en) | 2012-12-05 | 2016-08-03 | Exxonmobil Res & Eng Co | Nannochloropsis-KOZAK SEQUENCE |
| BR112015012811A2 (pt) | 2012-12-06 | 2017-11-14 | Exxonmobil Res & Eng Co | genes dgat compreendendo domínios de homologia de plecstrina e uso em micro-organismos recombinantes |
| EP2935364A2 (en) | 2012-12-21 | 2015-10-28 | Danisco US Inc. | Production of isoprene, isoprenoid, and isoprenoid precursors using an alternative lower mevalonate pathway |
| US10246694B2 (en) | 2013-04-10 | 2019-04-02 | Danisco Us Inc. | Phosphoketolases for improved production of acetyl coenzyme A-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids |
| WO2015127305A2 (en) | 2014-02-20 | 2015-08-27 | Danisco Us Inc. | Recombinant microorganisms for the enhanced production of mevalonate, isoprene, isoprenoid precursors, isoprenoids, and acetyl-coa-derived products |
| FR3041351B1 (fr) * | 2015-09-17 | 2020-01-24 | Eranova | Procede de preparation d'une poudre d'algues a teneur reduite en proteines et composition bioplastique formulee a partir d'une telle poudre |
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| WO2019113387A1 (en) | 2017-12-07 | 2019-06-13 | Zymergen Inc. | Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation |
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Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070065900A1 (en) * | 2005-09-19 | 2007-03-22 | Dicosimo Deana J | Process for chromosomal expression of foreign genes in the cytochrome-C peroxidase (cytCP) region of a methylotrophic microbial host cell |
| WO2007133558A2 (en) * | 2006-05-09 | 2007-11-22 | The Scripps Research Institute | Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast |
| CN101479378A (zh) * | 2006-06-29 | 2009-07-08 | 加利福尼亚大学董事会 | 使用经遗传工程改造的微观藻类、蓝细菌或细菌生产短链挥发烃类 |
| US7947478B2 (en) * | 2006-06-29 | 2011-05-24 | The Regents Of The University Of California | Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria |
| EP2142652B1 (en) * | 2007-04-03 | 2013-10-02 | Tabletop Energy | Methods for transforming eukaryotic algae |
| AU2008299020A1 (en) * | 2007-09-11 | 2009-03-19 | Sapphire Energy, Inc. | Molecule production by photosynthetic organisms |
| WO2009036385A2 (en) * | 2007-09-12 | 2009-03-19 | Kuehnle Agrosystems, Inc. | Expression of nucleic acid sequences for production of biofuels and other products in algae and cyanobacteria |
| CN101939423A (zh) * | 2007-10-05 | 2011-01-05 | 蓝宝石能源公司 | 用于捕获和修饰基因组dna大片段和用合成叶绿体构建生物体的系统 |
| WO2010051489A1 (en) * | 2008-10-31 | 2010-05-06 | Sapphire Energy, Inc. | Animal feedstock comprising genetically modified algae |
| WO2010078156A1 (en) * | 2008-12-31 | 2010-07-08 | Sapphire Energy, Inc. | Genetically engineered herbicide resistant algae |
| EP2406378A4 (en) * | 2009-03-11 | 2013-04-24 | Sapphire Energy Inc | BIOCARBURANT PRODUCTION IN PROCARYOTES AND EUKARYOTES |
| WO2010111707A1 (en) * | 2009-03-27 | 2010-09-30 | Sapphire Energy, Inc. | Variant isoprenoid producing enzymes and uses thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115820780A (zh) * | 2022-11-24 | 2023-03-21 | 云南爱尔发生物技术股份有限公司 | 一种利用褪黑素联合乙烯利促进栅藻积累叶黄素的方法 |
Also Published As
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| EP2477478A1 (en) | 2012-07-25 |
| EP2477478A4 (en) | 2013-02-13 |
| CN102630140A (zh) | 2012-08-08 |
| AU2010295739A1 (en) | 2012-03-08 |
| US20120252054A1 (en) | 2012-10-04 |
| WO2011034863A1 (en) | 2011-03-24 |
| IN2012DN01694A (enExample) | 2015-06-05 |
| SG179157A1 (en) | 2012-05-30 |
| AU2010295739B2 (en) | 2016-02-25 |
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