CN102549148A - 制备植物来源的vlp的方法 - Google Patents

制备植物来源的vlp的方法 Download PDF

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Publication number
CN102549148A
CN102549148A CN2010800423330A CN201080042333A CN102549148A CN 102549148 A CN102549148 A CN 102549148A CN 2010800423330 A CN2010800423330 A CN 2010800423330A CN 201080042333 A CN201080042333 A CN 201080042333A CN 102549148 A CN102549148 A CN 102549148A
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plant
vlps
fraction
protein
vlp
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Chinese (zh)
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路易斯-菲利普·韦齐纳
马农·科图雷
达尼·帕克特
米凯莱·达吉斯
马克-安德烈·德奥斯特
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Medicago Inc
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Medicago Inc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/517—Plant cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material

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  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Pulmonology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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CN2010800423330A 2009-09-22 2010-09-21 制备植物来源的vlp的方法 Pending CN102549148A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710164586.9A CN107129973A (zh) 2009-09-22 2010-09-21 制备植物来源的vlp的方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24478609P 2009-09-22 2009-09-22
US61/244,786 2009-09-22
PCT/CA2010/001488 WO2011035422A1 (en) 2009-09-22 2010-09-21 Method of preparing plant-derived vlps

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CN2010800423364A Pending CN102549008A (zh) 2009-09-22 2010-09-21 制备植物来源的蛋白质的方法
CN201710005957.9A Pending CN107090021A (zh) 2009-09-22 2010-09-21 制备植物来源的蛋白质的方法
CN2010800423330A Pending CN102549148A (zh) 2009-09-22 2010-09-21 制备植物来源的vlp的方法
CN201710164586.9A Pending CN107129973A (zh) 2009-09-22 2010-09-21 制备植物来源的vlp的方法

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CN2010800423364A Pending CN102549008A (zh) 2009-09-22 2010-09-21 制备植物来源的蛋白质的方法
CN201710005957.9A Pending CN107090021A (zh) 2009-09-22 2010-09-21 制备植物来源的蛋白质的方法

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EP (3) EP3354657B1 (enExample)
JP (2) JP5551780B2 (enExample)
KR (4) KR20180026562A (enExample)
CN (4) CN102549008A (enExample)
AR (2) AR078432A1 (enExample)
AU (2) AU2010300033B2 (enExample)
BR (2) BR112012006414B1 (enExample)
CA (2) CA2772964C (enExample)
DK (3) DK2480560T3 (enExample)
ES (3) ES2911037T3 (enExample)
HR (3) HRP20180509T1 (enExample)
HU (3) HUE036776T2 (enExample)
IL (4) IL218393A0 (enExample)
IN (2) IN2012DN02591A (enExample)
MX (2) MX2012003373A (enExample)
MY (2) MY164704A (enExample)
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PH (2) PH12012500566A1 (enExample)
PL (3) PL3354657T3 (enExample)
PT (3) PT2480560T (enExample)
RU (2) RU2579903C2 (enExample)
SG (2) SG178918A1 (enExample)
SI (3) SI2480658T1 (enExample)
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CN111727255A (zh) * 2017-11-30 2020-09-29 麦迪卡格公司 修饰后诺如病毒vp1蛋白和包含修饰后诺如病毒vp1蛋白的vlp

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CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
EP2238253B1 (en) 2007-11-27 2012-09-12 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
NZ597401A (en) 2009-06-24 2013-09-27 Medicago Inc Chimeric influenza virus-like particles comprising hemagglutinin
DK2480560T3 (en) * 2009-09-22 2018-04-16 Medicago Inc PROCEDURE FOR THE PREPARATION OF PLANT DERIVATIVE PROTEINS
TWI526539B (zh) 2010-12-22 2016-03-21 苜蓿股份有限公司 植物中生產類病毒顆粒(vlp)的方法及以該方法生產之vlp
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
KR101963792B1 (ko) * 2011-06-13 2019-07-31 메디카고 인코포레이티드 식물에서 광견병 바이러스 유사 입자 생성
US9441019B2 (en) * 2011-09-23 2016-09-13 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Influenza hemagglutinin protein-based vaccines
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
CN116218900A (zh) 2011-09-30 2023-06-06 麦迪卡格公司 增加植物中病毒样颗粒的产率
SG11201402657WA (en) * 2011-12-21 2014-09-26 Apse Llc Processes using vlps with capsids resistant to hydrolases
JP6406655B2 (ja) * 2012-02-22 2018-10-17 三菱商事フードテック株式会社 植物性食品素材の軟化方法、軟化製剤、軟化した植物性食品素材およびそれを用いた食品
WO2013142329A1 (en) * 2012-03-22 2013-09-26 Fraunhofer Usa, Inc. Virus -like particles comprising a matrix protein from a plant enveloped virus and uses thereof
MX364778B (es) 2012-09-05 2019-05-06 Medicago Inc Produccion de particulas similares a picornavirus en plantas.
US9617297B2 (en) * 2012-10-11 2017-04-11 The Regents Of The University Of California Apoplast wash fluid recovery for improved recombinant endoglucanase extraction in tabacco leaves
ES2803508T3 (es) * 2013-03-28 2021-01-27 Medicago Inc Producción de partículas similares al virus de la gripe en plantas
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
JP6358257B2 (ja) 2013-09-06 2018-07-18 三菱ケミカル株式会社 植物を用いたタンパク質の製造方法
EP3054971B1 (en) 2013-10-11 2021-03-10 The United States of America, represented by the Secretary, Department of Health and Human Services Epstein-barr virus vaccines
EP3092309B1 (en) 2014-01-10 2021-02-24 Medicago Inc. Cpmv enhancer elements
US11959088B2 (en) 2014-07-11 2024-04-16 Aramis Biotechnologies Inc. Modifying protein production in plants
SG11201705264WA (en) 2014-12-31 2017-07-28 The Usa As Represented By The Secretary Detp Of Health And Human Services Novel multivalent nanoparticle-based vaccines
JP2019129705A (ja) * 2016-03-31 2019-08-08 日本たばこ産業株式会社 植物に物質を導入する方法
EP3506938A1 (en) 2016-09-02 2019-07-10 The U.S.A. as represented by the Secretary, Department of Health and Human Services Stabilized group 2 influenza hemagglutinin stem region trimers and uses thereof
MX2020013957A (es) 2018-06-27 2021-03-09 Medicago Inc Mutantes de hemaglutinina del virus de la influenza.
JP7429684B6 (ja) * 2018-07-13 2024-03-15 メディカゴ インコーポレイテッド 改変ノロウイルスｖｐ１タンパク質および改変ノロウイルスｖｐ１タンパク質を含むｖｌｐ
CN109112093B (zh) * 2018-09-05 2021-11-09 上海交通大学 一种青蒿原生质体高效分离及瞬时转化的方法
MX2021012886A (es) * 2019-04-25 2022-01-04 Flagship Pioneering Innovations Vi Llc Composiciones y metodos relacionados con paquetes mensajeros de plantas.
CN110483616B (zh) * 2019-07-31 2021-06-04 中国农业科学院农产品加工研究所 从感染病原菌的植物组织中分离病原菌分泌的质外体效应蛋白的方法
CN111060696B (zh) * 2019-12-27 2023-09-08 湖南农业大学 一种降低植物小分子信号肽假阳性率的方法
CN111718394A (zh) * 2020-06-28 2020-09-29 广西壮族自治区农业科学院 一种基于bpp法的甘蔗组织变性蛋白提取方法
CN111826394A (zh) * 2020-08-07 2020-10-27 浙江华缔药业集团医药开发有限公司 植物瞬时表达载体的构建及应用
CN117479831A (zh) 2021-06-09 2024-01-30 南特知识产权控股有限责任公司 用于在植物中生产感兴趣的蛋白质的方法和系统
CN114934007B (zh) * 2022-07-01 2023-11-24 广东省农业科学院作物研究所 一种甘薯原生质体的制备方法
JP7745040B1 (ja) * 2024-06-21 2025-09-26 デンカ株式会社 目的タンパク質の回収方法及び製造方法
CN120082594B (zh) * 2025-04-07 2025-10-28 贵州大学 NbClpT2在调控植物抗PVY侵染中的应用

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