CN102449147B - 蛋白质间相互作用的高灵敏度检测方法 - Google Patents
蛋白质间相互作用的高灵敏度检测方法 Download PDFInfo
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- CN102449147B CN102449147B CN201080023492.6A CN201080023492A CN102449147B CN 102449147 B CN102449147 B CN 102449147B CN 201080023492 A CN201080023492 A CN 201080023492A CN 102449147 B CN102449147 B CN 102449147B
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Classifications
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- C—CHEMISTRY; METALLURGY
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
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- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
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- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009131481 | 2009-05-29 | ||
| JP2009-131481 | 2009-05-29 | ||
| JP2010-037921 | 2010-02-23 | ||
| JP2010037921A JP4849698B2 (ja) | 2009-05-29 | 2010-02-23 | タンパク質間相互作用の高感度検出方法 |
| PCT/JP2010/059160 WO2010137717A1 (ja) | 2009-05-29 | 2010-05-28 | タンパク質間相互作用の高感度検出方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102449147A CN102449147A (zh) | 2012-05-09 |
| CN102449147B true CN102449147B (zh) | 2014-11-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201080023492.6A Expired - Fee Related CN102449147B (zh) | 2009-05-29 | 2010-05-28 | 蛋白质间相互作用的高灵敏度检测方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US8470974B2 (enExample) |
| EP (1) | EP2436764B1 (enExample) |
| JP (1) | JP4849698B2 (enExample) |
| CN (1) | CN102449147B (enExample) |
| WO (1) | WO2010137717A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2016038750A1 (ja) * | 2014-09-10 | 2017-06-22 | オリンパス株式会社 | 分割型組換えルシフェラーゼ及びそれを用いた解析方法 |
| US10857228B2 (en) * | 2015-06-10 | 2020-12-08 | The University Of Tokyo | Adjuvant for vaccines, vaccine, and immunity induction method |
| CN104991072B (zh) * | 2015-06-16 | 2017-03-29 | 西北农林科技大学 | 一种昆虫体外蛋白质相互作用检测系统的制备方法及应用 |
| CN106916795B (zh) * | 2015-12-24 | 2021-12-10 | 中国科学院深圳先进技术研究院 | 一种可调节的荧光素酶分段融合蛋白、其制备方法及应用 |
| CA3016914C (en) * | 2016-03-15 | 2019-08-27 | Laboratory Corporation Of America Holdings | Methods of assessing protein interactions between cells |
| CN106928370B (zh) * | 2016-09-26 | 2021-02-02 | 华东师范大学 | 一种REGγ-20S蛋白酶体抑制剂的筛选系统及其应用 |
| CN116068198B (zh) * | 2022-11-30 | 2024-01-09 | 深圳湾实验室 | Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007027919A2 (en) * | 2005-09-01 | 2007-03-08 | Washington University In St. Louis | Complementation assays utilizing complexes of heteroproteins |
| EP1956086A1 (en) * | 2005-11-16 | 2008-08-13 | Toyo Boseki Kabushiki Kasisha | Luciferase gene optimized for use in imaging of intracellular luminescence |
| JP2008289475A (ja) * | 2007-04-27 | 2008-12-04 | National Institute Of Advanced Industrial & Technology | 最大発光波長がシフトした変異型ルシフェラーゼ |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7879540B1 (en) | 2000-08-24 | 2011-02-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| JP5083750B2 (ja) * | 2005-11-16 | 2012-11-28 | 独立行政法人産業技術総合研究所 | 細胞内発光イメージングのために最適化されたルシフェラーゼ遺伝子 |
| JP4648253B2 (ja) | 2006-06-22 | 2011-03-09 | 住友重機械テクノフォート株式会社 | 鍛造プレス |
| JP2009131481A (ja) | 2007-11-30 | 2009-06-18 | Ge Medical Systems Global Technology Co Llc | 外部操作パネルおよび超音波診断装置 |
| JP5258084B2 (ja) * | 2008-01-07 | 2013-08-07 | 株式会社ＰｒｏｂｅＸ | タンパク質間相互作用の検出方法 |
| JP2010004758A (ja) * | 2008-06-24 | 2010-01-14 | Probex Inc | ｃｙｃｌｉｃＧＭＰ検出方法 |
| JP2010037921A (ja) | 2008-08-04 | 2010-02-18 | Kadonaka Kogyo:Kk | 引戸の開閉装置 |
-
2010
- 2010-02-23 JP JP2010037921A patent/JP4849698B2/ja active Active
- 2010-05-28 CN CN201080023492.6A patent/CN102449147B/zh not_active Expired - Fee Related
- 2010-05-28 US US13/375,142 patent/US8470974B2/en not_active Expired - Fee Related
- 2010-05-28 WO PCT/JP2010/059160 patent/WO2010137717A1/ja not_active Ceased
- 2010-05-28 EP EP10780671.3A patent/EP2436764B1/en not_active Not-in-force
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2013
- 2013-05-31 US US13/906,475 patent/US20130323814A1/en not_active Abandoned
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2014
- 2014-01-31 US US14/169,750 patent/US9540678B2/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007027919A2 (en) * | 2005-09-01 | 2007-03-08 | Washington University In St. Louis | Complementation assays utilizing complexes of heteroproteins |
| EP1956086A1 (en) * | 2005-11-16 | 2008-08-13 | Toyo Boseki Kabushiki Kasisha | Luciferase gene optimized for use in imaging of intracellular luminescence |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN102449147A (zh) | 2012-05-09 |
| EP2436764B1 (en) | 2015-04-22 |
| EP2436764A1 (en) | 2012-04-04 |
| WO2010137717A1 (ja) | 2010-12-02 |
| US20120190824A1 (en) | 2012-07-26 |
| US9540678B2 (en) | 2017-01-10 |
| JP2011004734A (ja) | 2011-01-13 |
| JP4849698B2 (ja) | 2012-01-11 |
| US8470974B2 (en) | 2013-06-25 |
| EP2436764A4 (en) | 2012-11-28 |
| US20130323814A1 (en) | 2013-12-05 |
| US20140141416A1 (en) | 2014-05-22 |
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