CN102449147B - 蛋白质间相互作用的高灵敏度检测方法 - Google Patents

蛋白质间相互作用的高灵敏度检测方法 Download PDF

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Publication number
CN102449147B
CN102449147B CN201080023492.6A CN201080023492A CN102449147B CN 102449147 B CN102449147 B CN 102449147B CN 201080023492 A CN201080023492 A CN 201080023492A CN 102449147 B CN102449147 B CN 102449147B
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fusion rotein
seq
protein
sequence shown
aminoacid sequence
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Chinese (zh)
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CN102449147A (zh
Inventor
小泽岳昌
三泽直美
三浦研二
冈本将
西井重明
增田兼治
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Probex Inc
University of Tokyo NUC
Toyo Textile Co Ltd
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Probex Inc
University of Tokyo NUC
Toyo Textile Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN201080023492.6A 2009-05-29 2010-05-28 蛋白质间相互作用的高灵敏度检测方法 Expired - Fee Related CN102449147B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2009-131481 2009-05-29
JP2009131481 2009-05-29
JP2010037921A JP4849698B2 (ja) 2009-05-29 2010-02-23 タンパク質間相互作用の高感度検出方法
JP2010-037921 2010-02-23
PCT/JP2010/059160 WO2010137717A1 (ja) 2009-05-29 2010-05-28 タンパク質間相互作用の高感度検出方法

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CN102449147A CN102449147A (zh) 2012-05-09
CN102449147B true CN102449147B (zh) 2014-11-12

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US (3) US8470974B2 (enExample)
EP (1) EP2436764B1 (enExample)
JP (1) JP4849698B2 (enExample)
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WO (1) WO2010137717A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2016038750A1 (ja) * 2014-09-10 2017-06-22 オリンパス株式会社 分割型組換えルシフェラーゼ及びそれを用いた解析方法
EP3308800B1 (en) * 2015-06-10 2021-08-25 The University of Tokyo Adjuvant for vaccines, vaccine, and immunity induction method
CN104991072B (zh) * 2015-06-16 2017-03-29 西北农林科技大学 一种昆虫体外蛋白质相互作用检测系统的制备方法及应用
CN106916795B (zh) * 2015-12-24 2021-12-10 中国科学院深圳先进技术研究院 一种可调节的荧光素酶分段融合蛋白、其制备方法及应用
JP2019515252A (ja) * 2016-03-15 2019-06-06 ラボラトリー コーポレイション オブ アメリカ ホールディングス 細胞間のタンパク質相互作用を評価する方法
CN106928370B (zh) * 2016-09-26 2021-02-02 华东师范大学 一种REGγ-20S蛋白酶体抑制剂的筛选系统及其应用
CN116068198B (zh) * 2022-11-30 2024-01-09 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007027919A2 (en) * 2005-09-01 2007-03-08 Washington University In St. Louis Complementation assays utilizing complexes of heteroproteins
EP1956086A1 (en) * 2005-11-16 2008-08-13 Toyo Boseki Kabushiki Kasisha Luciferase gene optimized for use in imaging of intracellular luminescence
JP2008289475A (ja) * 2007-04-27 2008-12-04 National Institute Of Advanced Industrial & Technology 最大発光波長がシフトした変異型ルシフェラーゼ

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP5083750B2 (ja) * 2005-11-16 2012-11-28 独立行政法人産業技術総合研究所 細胞内発光イメージングのために最適化されたルシフェラーゼ遺伝子
JP4648253B2 (ja) 2006-06-22 2011-03-09 住友重機械テクノフォート株式会社 鍛造プレス
JP2009131481A (ja) 2007-11-30 2009-06-18 Ge Medical Systems Global Technology Co Llc 外部操作パネルおよび超音波診断装置
JP5258084B2 (ja) * 2008-01-07 2013-08-07 株式会社ＰｒｏｂｅＸ タンパク質間相互作用の検出方法
JP2010004758A (ja) * 2008-06-24 2010-01-14 Probex Inc ｃｙｃｌｉｃＧＭＰ検出方法
JP2010037921A (ja) 2008-08-04 2010-02-18 Kadonaka Kogyo:Kk 引戸の開閉装置

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007027919A2 (en) * 2005-09-01 2007-03-08 Washington University In St. Louis Complementation assays utilizing complexes of heteroproteins
EP1956086A1 (en) * 2005-11-16 2008-08-13 Toyo Boseki Kabushiki Kasisha Luciferase gene optimized for use in imaging of intracellular luminescence
JP2008289475A (ja) * 2007-04-27 2008-12-04 National Institute Of Advanced Industrial & Technology 最大発光波長がシフトした変異型ルシフェラーゼ

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Circularly Permutated Bioluminescent Probes for Illuminating Ligand-Activated Protein Dynamics;Sung Bae Kim, et al.;《Bioconjugate Chem.》;20081202;第19卷;2480-2486 *
High-Sensitivity Real-Time Imaging of Dual Protein-Protein Interactions in Living Subjects Using Multicolor Luciferases;Naoki Hida, et al.;《PLOS one》;20090612;第4卷(第6期);1-12 *
High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase;Sung Bae Kim, et al.;《PNAS》;20040810;第101卷(第32期);11542-11547 *
Imaging CXCR4 Signaling with Firefly Luciferase Complementation;Kathryn E. Luker, et al.;《Anal. Chem.》;20080606;第80卷(第14期);5565-5573 *
Rapid and High-Sensitivity Cell-Based Assays of Protein-Protein Interactions Using Split Click Beetle Luciferase Complementation: An Approach to the Study of G-Protein-Coupled Receptors;Naomi Misawa, et al.;《Anal. Chem.》;20100315;第82卷;2552-2560 *

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Publication number Publication date
WO2010137717A1 (ja) 2010-12-02
US9540678B2 (en) 2017-01-10
US20120190824A1 (en) 2012-07-26
US20140141416A1 (en) 2014-05-22
US8470974B2 (en) 2013-06-25
US20130323814A1 (en) 2013-12-05
EP2436764A1 (en) 2012-04-04
JP4849698B2 (ja) 2012-01-11
JP2011004734A (ja) 2011-01-13
EP2436764B1 (en) 2015-04-22
CN102449147A (zh) 2012-05-09
EP2436764A4 (en) 2012-11-28

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