PL450655A1 - System fragmentów lucyferazy z uniwersalnymi adapterami przeciwciał i białek do homogenicznej detekcji substancji w próbce lub testów interakcji molekularnych - Google Patents

System fragmentów lucyferazy z uniwersalnymi adapterami przeciwciał i białek do homogenicznej detekcji substancji w próbce lub testów interakcji molekularnych

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Publication number
PL450655A1
PL450655A1 PL450655A PL45065524A PL450655A1 PL 450655 A1 PL450655 A1 PL 450655A1 PL 450655 A PL450655 A PL 450655A PL 45065524 A PL45065524 A PL 45065524A PL 450655 A1 PL450655 A1 PL 450655A1
Authority
PL
Poland
Prior art keywords
samples
substances
molecular interaction
homogeneous detection
interaction assays
Prior art date
Application number
PL450655A
Other languages
English (en)
Inventor
Marzena KRZEK
Grażyna WASIAK-MACIEJEWSKA
Anna SZULC-KAPUŚCIŃSKA
Michał KOPCIAŁ
Piotr SKLEPKIEWICZ
Rafał Kozieł
Łukasz Krzemiński
Original Assignee
Echia Lab Spółka Z Ograniczoną Odpowiedzialnością
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Echia Lab Spółka Z Ograniczoną Odpowiedzialnością filed Critical Echia Lab Spółka Z Ograniczoną Odpowiedzialnością
Priority to PL450655A priority Critical patent/PL450655A1/pl
Priority to PCT/PL2025/050104 priority patent/WO2026135483A1/en
Publication of PL450655A1 publication Critical patent/PL450655A1/pl

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Plasma & Fusion (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia są nowe adaptery białkowe i ich fuzje z fragmentami enzymu reporterowego lucyferazy FLuc oraz sposoby ustalania obecności wykrywanej substancji w badanej próbce, a także badania interakcji dwóch molekuł.
PL450655A 2024-12-20 2024-12-20 System fragmentów lucyferazy z uniwersalnymi adapterami przeciwciał i białek do homogenicznej detekcji substancji w próbce lub testów interakcji molekularnych PL450655A1 (pl)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL450655A PL450655A1 (pl) 2024-12-20 2024-12-20 System fragmentów lucyferazy z uniwersalnymi adapterami przeciwciał i białek do homogenicznej detekcji substancji w próbce lub testów interakcji molekularnych
PCT/PL2025/050104 WO2026135483A1 (en) 2024-12-20 2025-12-22 Luciferase fragment system with universal antibody and protein adapters for homogeneous detection of substances in a sample or molecular interaction assays

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450655A PL450655A1 (pl) 2024-12-20 2024-12-20 System fragmentów lucyferazy z uniwersalnymi adapterami przeciwciał i białek do homogenicznej detekcji substancji w próbce lub testów interakcji molekularnych

Publications (1)

Publication Number Publication Date
PL450655A1 true PL450655A1 (pl) 2026-06-22

Family

ID=98868919

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450655A PL450655A1 (pl) 2024-12-20 2024-12-20 System fragmentów lucyferazy z uniwersalnymi adapterami przeciwciał i białek do homogenicznej detekcji substancji w próbce lub testów interakcji molekularnych

Country Status (2)

Country Link
PL (1) PL450655A1 (pl)
WO (1) WO2026135483A1 (pl)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009087967A1 (en) * 2008-01-07 2009-07-16 Probex Inc. Method for detecting a protein-protein interaction
EP2436764A1 (en) * 2009-05-29 2012-04-04 The University of Tokyo Method for highly sensitive detection of protein-protein interaction
WO2016038750A1 (ja) * 2014-09-10 2016-03-17 オリンパス株式会社 分割型組換えルシフェラーゼ及びそれを用いた解析方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009087967A1 (en) * 2008-01-07 2009-07-16 Probex Inc. Method for detecting a protein-protein interaction
EP2436764A1 (en) * 2009-05-29 2012-04-04 The University of Tokyo Method for highly sensitive detection of protein-protein interaction
WO2016038750A1 (ja) * 2014-09-10 2016-03-17 オリンパス株式会社 分割型組換えルシフェラーゼ及びそれを用いた解析方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
C. OKER-BLOM ET AL.: "Biotechniques. 1993 May; 14(5): 800-9", A BACULOVIRUS-EXPRESSED FUSION PROTEIN CONTAINING THE ANTIBODY-BINDING DOMAIN OF PROTEIN A AND INSECT LUCIFERASE. *
LI J-F ET AL.: "PLoS ONE, 2011; 6(11): e27364", LARGE-SCALE PROTEIN-PROTEIN INTERACTION ANALYSIS IN ARABIDOPSIS MESOPHYLL PROTOPLASTS BY SPLIT FIREFLY LUCIFERASE COMPLEMENTATION. *

Also Published As

Publication number Publication date
WO2026135483A1 (en) 2026-06-25