JP4849698B2 - タンパク質間相互作用の高感度検出方法 - Google Patents

タンパク質間相互作用の高感度検出方法 Download PDF

Info

Publication number
JP4849698B2
JP4849698B2 JP2010037921A JP2010037921A JP4849698B2 JP 4849698 B2 JP4849698 B2 JP 4849698B2 JP 2010037921 A JP2010037921 A JP 2010037921A JP 2010037921 A JP2010037921 A JP 2010037921A JP 4849698 B2 JP4849698 B2 JP 4849698B2
Authority
JP
Japan
Prior art keywords
fusion protein
protein
amino acid
acid sequence
complex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2010037921A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011004734A5 (enExample
JP2011004734A (ja
Inventor
岳昌 小澤
直美 三澤
研二 三浦
岡本　　将
重明 西井
兼治 増田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Probex
Original Assignee
Probex
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2010037921A priority Critical patent/JP4849698B2/ja
Application filed by Probex filed Critical Probex
Priority to PCT/JP2010/059160 priority patent/WO2010137717A1/ja
Priority to US13/375,142 priority patent/US8470974B2/en
Priority to CN201080023492.6A priority patent/CN102449147B/zh
Priority to EP10780671.3A priority patent/EP2436764B1/en
Publication of JP2011004734A publication Critical patent/JP2011004734A/ja
Publication of JP2011004734A5 publication Critical patent/JP2011004734A5/ja
Application granted granted Critical
Publication of JP4849698B2 publication Critical patent/JP4849698B2/ja
Priority to US13/906,475 priority patent/US20130323814A1/en
Priority to US14/169,750 priority patent/US9540678B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2010037921A 2009-05-29 2010-02-23 タンパク質間相互作用の高感度検出方法 Active JP4849698B2 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2010037921A JP4849698B2 (ja) 2009-05-29 2010-02-23 タンパク質間相互作用の高感度検出方法
US13/375,142 US8470974B2 (en) 2009-05-29 2010-05-28 Method for highly sensitive detection of protein-protein interaction
CN201080023492.6A CN102449147B (zh) 2009-05-29 2010-05-28 蛋白质间相互作用的高灵敏度检测方法
EP10780671.3A EP2436764B1 (en) 2009-05-29 2010-05-28 Method for highly sensitive detection of protein-protein interaction
PCT/JP2010/059160 WO2010137717A1 (ja) 2009-05-29 2010-05-28 タンパク質間相互作用の高感度検出方法
US13/906,475 US20130323814A1 (en) 2009-05-29 2013-05-31 Method for highly sensitive detection of protein-protein interaction
US14/169,750 US9540678B2 (en) 2009-05-29 2014-01-31 Method for highly sensitive detection of protein-protein interaction

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009131481 2009-05-29
JP2009131481 2009-05-29
JP2010037921A JP4849698B2 (ja) 2009-05-29 2010-02-23 タンパク質間相互作用の高感度検出方法

Publications (3)

Publication Number Publication Date
JP2011004734A JP2011004734A (ja) 2011-01-13
JP2011004734A5 JP2011004734A5 (enExample) 2011-09-22
JP4849698B2 true JP4849698B2 (ja) 2012-01-11

Family

ID=43222819

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010037921A Active JP4849698B2 (ja) 2009-05-29 2010-02-23 タンパク質間相互作用の高感度検出方法

Country Status (5)

Country Link
US (3) US8470974B2 (enExample)
EP (1) EP2436764B1 (enExample)
JP (1) JP4849698B2 (enExample)
CN (1) CN102449147B (enExample)
WO (1) WO2010137717A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2016038750A1 (ja) * 2014-09-10 2017-06-22 オリンパス株式会社 分割型組換えルシフェラーゼ及びそれを用いた解析方法
US10857228B2 (en) * 2015-06-10 2020-12-08 The University Of Tokyo Adjuvant for vaccines, vaccine, and immunity induction method
CN104991072B (zh) * 2015-06-16 2017-03-29 西北农林科技大学 一种昆虫体外蛋白质相互作用检测系统的制备方法及应用
CN106916795B (zh) * 2015-12-24 2021-12-10 中国科学院深圳先进技术研究院 一种可调节的荧光素酶分段融合蛋白、其制备方法及应用
CA3016914C (en) * 2016-03-15 2019-08-27 Laboratory Corporation Of America Holdings Methods of assessing protein interactions between cells
CN106928370B (zh) * 2016-09-26 2021-02-02 华东师范大学 一种REGγ-20S蛋白酶体抑制剂的筛选系统及其应用
CN116068198B (zh) * 2022-11-30 2024-01-09 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
WO2007027919A2 (en) * 2005-09-01 2007-03-08 Washington University In St. Louis Complementation assays utilizing complexes of heteroproteins
JP5083750B2 (ja) * 2005-11-16 2012-11-28 独立行政法人産業技術総合研究所 細胞内発光イメージングのために最適化されたルシフェラーゼ遺伝子
US8383797B2 (en) * 2005-11-16 2013-02-26 Toyo Boseki Kabushiki Kaisha Luciferase gene optimized for use in imaging of intracellular luminescence
JP4648253B2 (ja) 2006-06-22 2011-03-09 住友重機械テクノフォート株式会社 鍛造プレス
JP2008289475A (ja) * 2007-04-27 2008-12-04 National Institute Of Advanced Industrial & Technology 最大発光波長がシフトした変異型ルシフェラーゼ
JP2009131481A (ja) 2007-11-30 2009-06-18 Ge Medical Systems Global Technology Co Llc 外部操作パネルおよび超音波診断装置
JP5258084B2 (ja) * 2008-01-07 2013-08-07 株式会社ＰｒｏｂｅＸ タンパク質間相互作用の検出方法
JP2010004758A (ja) * 2008-06-24 2010-01-14 Probex Inc ｃｙｃｌｉｃＧＭＰ検出方法
JP2010037921A (ja) 2008-08-04 2010-02-18 Kadonaka Kogyo:Kk 引戸の開閉装置

Also Published As

Publication number Publication date
CN102449147A (zh) 2012-05-09
EP2436764B1 (en) 2015-04-22
EP2436764A1 (en) 2012-04-04
CN102449147B (zh) 2014-11-12
WO2010137717A1 (ja) 2010-12-02
US20120190824A1 (en) 2012-07-26
US9540678B2 (en) 2017-01-10
JP2011004734A (ja) 2011-01-13
US8470974B2 (en) 2013-06-25
EP2436764A4 (en) 2012-11-28
US20130323814A1 (en) 2013-12-05
US20140141416A1 (en) 2014-05-22

Similar Documents

Publication Publication Date Title
US12366572B2 (en) Activation of bioluminescence by structural complementation
JP4849698B2 (ja) タンパク質間相互作用の高感度検出方法
JP4955391B2 (ja) Ｇタンパク質共役受容体およびそのシグナル伝達経路の断片相補アッセイ
Wouters et al. Luminescence-and fluorescence-based complementation assays to screen for GPCR oligomerization: current state of the art
Rebois et al. Combining protein complementation assays with resonance energy transfer to detect multipartner protein complexes in living cells
US11360096B2 (en) Complex BRET technique for measuring biological interactions
JPWO2010119721A1 (ja) 超高輝度で安定な人工生物発光酵素
US8124424B2 (en) Single molecule-format bioluminescent probe
AU2013229528A1 (en) Membrane span-kinase fusion protein and the uses thereof
JP2009529893A (ja) タンパク質−タンパク質相互作用のアッセイ方法
WO2022245922A1 (en) Poly-adp ribose (par) tracker optimized split-protein reassembly par detection reagents
US20220404337A1 (en) Real-time g-protein coupled receptor (gpcr) linked bioluminescent sensing of biological targets and processes
JP6525199B2 (ja) インスリンの検出方法、および、インスリンの検出キット
JP2010178676A (ja) 生物発光・蛍光プローブ用アンカーペプチド
WO2005085439A1 (ja) 蛋白質核内移行検出用プローブとそれを用いた蛋白質核内移行の検出・定量方法
Kim et al. Circular Permutation Probes for Illuminating Phosphorylation of Estrogen Receptor

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110801

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20110801

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20110801

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20110801

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20110907

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20111011

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20111017

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

Ref document number: 4849698

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20141028

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250