CN102432352B - Biological synergist - Google Patents
Biological synergist Download PDFInfo
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- CN102432352B CN102432352B CN201110308064.4A CN201110308064A CN102432352B CN 102432352 B CN102432352 B CN 102432352B CN 201110308064 A CN201110308064 A CN 201110308064A CN 102432352 B CN102432352 B CN 102432352B
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Abstract
The invention discloses a biological synergist, and belongs to the field of biological organic fertilizers. The effective viable count of the biological synergist is more than or equal to 0.2 billion/gram, the miscellaneous bacterium rate is less than or equal to 15 percent, the PH value is 5 to 7, and the validity period is not shorter than half a year. The invention has the advantages that: the biological synergist comprises beneficial microorganisms such as nitrogen-fixing bacteria, spore bacteria, a liquid bacterial agent, a freeze-dried bacterial agent, an oil-dried bacterial agent and the like which have the functions of decomposing organic substances, phosphorus and potassium in soil and fertilizers to increase the nitrogen, phosphorus and potassium nutrition of crops, resisting crop diseases, and the like; and a multi-element double-season compound fertilizer or a biological compound fertilizer prepared by matching the beneficial microorganisms with organic fertilizers and inorganic fertilizers is a fully functional fertilizer, the utilization rate of the fertilizer can be improved, the yield of the crops is increased, the water and fertility retaining capacity of the soil can be improved, land using and land nourishing are combined, the application of pesticides and chemical fertilizers can be reduced, agricultural input is reduced, pollution to agricultural environment is reduced, the quality safety of agricultural products can be improved, and agricultural yield is further improved.
Description
Technical field
The present invention relates to a kind of biological organic fertilizer, particularly a kind of biological potentiating agent.
Background technology
Existing fertilizer, inorganic fertilizer, the utilization ratio of fertilizer is not high, can not guarantee crop yield, not strong for soil water-retaining, fertilizer-preserving ability, can not realize land used and combining of increasing soil fertility, if but use frequently, not only agricultural has high input, also very large to the pollution of agricultural environment, can not guarantee the quality safety of agricultural-food, can not guarantee the raising of farm output simultaneously.
Biological potentiating agent claims again bacterial manure or biological organic fertilizer, microorganism fertilizer, is the Agricultural products that a class contains live microorganism, take nutrition as main, take disease-resistantly as main, take degrading pesticide as main, also can be used for multiple effect dual-purpose simultaneously.Biological potentiating agent is that one or more microorganisms (vinelandii, sporeformer or some other bacterium) are favourable mutually, by its vital movement, make crop yield, each quasi-microorganism has in planting process the plant stimulin of self can refuse more anti-pathogenic bacterias numerous, reach the object, the especially soil-borne disease that suppress disease.Such as: shallow parasitosis, gaeumannomyces graminis disease, bacterial wilt, blight etc., some bacterium energy activating soils, fixing phosphorus, potassium element, make it to be absorbed by plants, rotten hot promotor, some microbial inoculums can accelerate the rotten heat of crop straw and promote organic waste fermentation, and three major polymers: cellulose decomposer forms, biological potentiating agent and organism are compound, and microorganism and inorganics are compound.
Summary of the invention
Object of the present invention is just to provide a kind of biological organic fertilizer, in this kind of biological potentiating agent, contain the beneficial microorganisms such as vinelandii, sporeformer, liquid bacterial agent, freeze-drying microbial inoculum, oily dry microbial inoculum, coordinating multielement two-shipper composite fertilizer or the biological compound fertilizer made from fertilizer, inorganic fertilizer, is that global function is fertile.
To achieve these goals, the technical solution used in the present invention is such: a kind of biological potentiating agent, and the living bacteria count that it contains >=200,000,000/gram, miscellaneous bacteria rate≤15%, pH value is 5-7, validity period is not less than half a year.
A preparation method who prepares described biological potentiating agent, comprises the following steps:
(1) prepare burden:
30 parts of rough rice shells, 30 parts of rice brans, 20 parts of wheat bran, 5 parts of careless carbon, 5 parts of weathered coals, 4-5 part beneficial microorganism activators are carried out disinfection respectively, then mix and be mixed evenly, obtain culture material;
The sterilization method that each raw material adopts is the ozonization producing with trioxide disinfecting machine or steams (6-10ml/m with formaldehyde
3) sterilization;
(2) regulate humidity and PH:
The sodium hydroxide of 0.3-0.4 part is fully dissolved in 4.5 parts of water, to be dissolved completely after, be sprayed on the culture material of gained in step (1), fully mix be mixed even, can not have wet group, with the paper that extensively shows of PH1-14, test that to make PH be 7, water content is 40-50%.
(3) pack:
By step (2) in cloth bag or woven bag charging for the culture material of gained, every packed 10kg, then with the tying of restricting;
(4) steaming sterilizing:
Adopt direct steaming method, use normal-pressure sterilization method or autoclaving to step (3) in the culture material of pack carry out sterilizing;
(5) cultivation and fermentation:
A, proving room are prepared: at least prepare two, require the airtight sterilization of energy, insulation, ventilative, the heat radiation of windowing again, before production, need to proving room sweep, the sterilization of dedusting, brush lime white, then the ozonization producing with trioxide disinfecting machine or steam (6-10ml/m with formaldehyde
3) sterilization; ;
B, one grade fermemtation: sterilized culture material is pressed to 10% of gained biological potentiating agent gross weight, put into an indirect fermentation indoor, 3-4 ℃ to be chilled to, paves material, inoculation 4-5 part beneficial microorganism activator, abundant and culture material mixes, heap fermentation, piles thick 20-25cm, and heap temperature remains on 40-45 ℃, ferment 24 hours, obtain one grade fermemtation bacterium;
C, the fermentation of secondary enlarged culturing: by remaining sterilized culture material, put into another proving room of having sterilized, treat that material temperature is down to 45 ℃, material is paved to the one grade fermemtation bacterium of gained in inoculation step (5) B, fully mix, fermentation reactor system, piles thick 20-25cm, and heap temperature remains on 40-45 ℃, fermentation 16-24 hour, obtains biological potentiating agent;
(6) discharging, dry:
Biological potentiating agent is spread on the place ventilating in solarization instrument or surrounding, make thinner, stir, dry, airing;
(7) pack, store:
Dried biological potentiating agent, by quantitatively making small packages with plastics bag, also available multiple film woven bag is made large packing, is stored in health, cool place, dry, ventilation.
As preferably, the normal-pressure sterilization method of described step in (4) refers to and in steamer, adds water, heating, utilizes the high-temperature steam producing to carry out sterilizing, in airtight steamer or food steamer, passes into steam, when vapor temperature reaches 100 ℃, starts timing, sterilizing 2 hours; Described autoclaving refers to puts into cultivation pocket in high-pressure sterilizing pot or sterilizing cabinet, at pressure, is 1 normal atmosphere/cm
2, sterilizing is 1 hour at 121 ℃.
The beneficial microorganism activator that wherein used contains the beneficial microorganisms such as vinelandii, sporeformer, liquid bacterial agent, freeze-drying microbial inoculum, oily dry microbial inoculum.
Compared with prior art, the invention has the beneficial effects as follows: in this kind of biological potentiating agent, contain the beneficial microorganisms such as vinelandii, sporeformer, liquid bacterial agent, freeze-drying microbial inoculum, oily dry microbial inoculum, they have organic substance, minute phosphorus decomposing, minute potassium decomposing of decomposing in soil and fertilizer, to increase nitrogen, phosphorus, the potassium nutrition of crop, the functions such as anti-crop pest.Therefore, they are coordinated to multielement two-shipper composite fertilizer or the biological compound fertilizer made from fertilizer, inorganic fertilizer, be that global function is fertile, can improve the utilization ratio of fertilizer, guarantee crop yield, can improve soil water-retaining, fertilizer-preserving ability again, realizing land used combines with increasing soil fertility, can also reduce agricultural chemicals, chemical fertilizer application, both reduce agriculture input, reduce again the pollution to agricultural environment.Can also improve the quality safety of agricultural-food, guarantee that again farm output further improves.
Through field, contrast, with single nitrogen, phosphorus, potassium, organic matter, compare with biological potentiating agent, biological potentiating agent can reduce agricultural and drop into (110 yuan every mu), mu volume increase 18-23%, reduced the pollution to agricultural environment, can improve the quality safety of agricultural-food again, biological potentiating agent is a kind of plant environmental protection enhanced fertilizer.
Accompanying drawing explanation
Fig. 1 is preparation method's of the present invention process flow sheet.
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described.
Process flow sheet as shown in Figure 1, a kind of biological potentiating agent, the living bacteria count that it contains >=200,000,000/gram, miscellaneous bacteria rate≤15%, pH value is 5-7, validity period is not less than half a year.
A preparation method who prepares described biological potentiating agent, comprises the following steps:
By the amount of producing 1000Kg, prepare:
(1) prepare burden:
300Kg rough rice shell, 300Kg rice bran, 200Kg wheat bran, 50Kg grass carbon, 50Kg weathered coal, 40-50Kg beneficial microorganism activator are carried out disinfection respectively, then mix and be mixed evenly, obtain culture material;
The sterilization method that each raw material adopts is the ozonization producing with trioxide disinfecting machine;
(2) regulate humidity and PH:
The sodium hydroxide of 3-4Kg is fully dissolved in 45Kg water, to be dissolved completely after, be sprayed on the culture material of gained in step (1), fully mix and be mixed evenly, can not have wet group, with the paper that extensively shows of PH1-14, test that to make PH be 7, water content is 40-50%, and general manual energy is agglomerating, looses one's grip loose;
When regulating the pH value and water content of culture material, the consumption of sodium hydroxide and water should be determined according to the dry wet and PH of raw material;
(3) pack:
By step (2) in cloth bag or woven bag charging for the culture material of gained, every packed 10kg, then with rope tying, when installing, should not fill too much, tension, in order to avoid affect sterilising effect;
(4) steaming sterilizing:
Adopt direct steaming method, use normal-pressure sterilization method to step (3) in the culture material of pack carry out sterilizing; Described normal-pressure sterilization method refers to and in steamer, adds water, heating, utilizes the high-temperature steam producing to carry out sterilizing, in airtight steamer or food steamer, passes into steam, when vapor temperature reaches 100 ℃, starts timing, sterilizing 2 hours;
The object of steaming is to make culture material thoroughly without miscellaneous bacteria, and accelerates the culture material conversion of becoming thoroughly decomposed, and to probiotics, creates good growing environment, so the important step that steaming sterilizing is bacterium chaff in producing;
(5) cultivation and fermentation:
A, proving room are prepared: at least prepare two, require the airtight sterilization of energy, insulation, ventilative, the heat radiation of windowing again, before production, need to proving room sweep, the sterilization of dedusting, brush lime white, then the ozonization producing with trioxide disinfecting machine or steam (6-10ml/m with formaldehyde
3) sterilization;
B, one grade fermemtation: sterilized culture material is pressed to 10% of gained biological potentiating agent gross weight, put into an indirect fermentation indoor, 3-4 ℃ to be chilled to, material is paved, inoculation 40-50Kg beneficial microorganism activator, abundant and culture material mixes, heap fermentation, pile thick 20-25cm, (summer can be thin, winter can be thick), when temperature is low, note heating up and cultivate, when temperature is high, note ventilating, cooling and moisturizing, after composting, approximately 4 hours temperature rise gradually, to 45 ℃ of left and right, start to produce fragrance, adopt the method for stirring to regulate temperature, heap temperature remains on 40-45 ℃, ferment 24 hours, obtain one grade fermemtation bacterium,
Ripe one grade fermemtation bacterium has peat-reek, very loose, soft, and this if find tart flavour, vinosity, stink, by living contaminants, can not use the bacterial classification as fermenting for the second time as bacterial classification;
C, the fermentation of secondary enlarged culturing: by remaining sterilized culture material, put into another proving room of having sterilized, treat that material temperature is down to 45 ℃, material is paved, and in inoculation step (5) B, the one grade fermemtation bacterium of gained, fully mixes, fermentation reactor system, pile thick 20-25cm, heap temperature remains on 40-45 ℃, but second order fermentation heats up comparatively fast, need duty to stir, when temperature is high, heap temperature is difficult to remain on 40-45 ℃, now needs door window to ventilate, breathe freely, reinforcement is stirred, to maintain the temperature at 40-45 ℃, fermentation 16-24 hour, obtains biological potentiating agent;
With regard to savory generation, ripe biological potentiating agent fragrance was denseer, was clamminess or had filament caking etc. in 6-7 hour for the normal general cultivation of fermentation;
(6) discharging, dry:
The biological potentiating agent of gained is spread on the place of solarization instrument or surrounding ventilation, make thinner, need duty to stir, dry, airing, make it to be dried, must guard against stacking, now desirable biological potentiating agent detects, survey effective bacteria containing amount and miscellaneous bacteria rate, its product index is: living bacteria count >=200,000,000/gram, miscellaneous bacteria rate≤15%, pH value is 5-7.
(7) pack, store:
Dried biological potentiating agent, by quantitatively making small packages with plastics bag, also available multiple film woven bag is made large packing, is stored in health, cool place, dry, ventilation, must guard against and exposes to the sun and rain.
When producing the agent of proliferation effect in order to prevent in air, the pollution of miscellaneous bacteria on object to culture material, so will be to culture material, culturing room in production process, the instrument using etc. carries out disinfection and sterilizing, producers also will carry out aseptic technique at work, comprise and wear working suit and footwear, the cap etc. of having sterilized.In order to guarantee beneficial microorganism, cultivate successfully, in production, must meet the living condition of these microorganisms, comprise nutrition, moisture content, PH, air, temperature, therefore, in process of production must accurate feed proportioning, regulate moisture, PH, at proving room, conscientiously perform that temperature control, control between yeast phase is wet, ventilation, ventilative management.
Industry is used and is produced fertilizer, composite fertilizer consumption 8-13 kilogram per ton; Agricultural application: grain is 2-3 kilogram for mu; Vegetables are 4-5 kilogram for mu; Fruit is 5-8 kilogram for mu; Can mix and use with phosphorus, potash fertilizer.
Through field, contrast, with single nitrogen, phosphorus, potassium, organic matter, compare with biological potentiating agent, biological potentiating agent can reduce agricultural and drop into (110 yuan every mu), mu volume increase 18-23%, reduced the pollution to agricultural environment, can improve the quality safety of agricultural-food again, biological potentiating agent is a kind of plant environmental protection enhanced fertilizer.
Claims (1)
1. a preparation method for biological potentiating agent, is characterized in that comprising the following steps:
(1) prepare burden:
30 parts of rough rice shells, 30 parts of rice brans, 20 parts of wheat bran, 5 parts of careless carbon, 5 parts of weathered coals, 4-5 part beneficial microorganism activators are carried out disinfection respectively, then mix and be mixed evenly, obtain culture material;
The sterilization method that each raw material adopts is the ozonization producing with trioxide disinfecting machine;
(2) regulate humidity and pH:
The sodium hydroxide of 0.3-0.4 part is fully dissolved in 4.5 parts of water, to be dissolved completely after, be sprayed on the culture material of gained in step (1), fully mix be mixed even, can not have wet group, test that to make pH be 7 with the extensive test paper of pH1-14, water content is 40-50%;
(3) pack:
By step (2) in cloth bag or woven bag charging for the culture material of gained, every packed 10kg, then with the tying of restricting;
(4) steaming sterilizing:
Adopt direct steaming method, use normal-pressure sterilization method to step (3) in the culture material of pack carry out sterilizing;
(5) cultivation and fermentation:
A, proving room prepare: at least prepare two, requiring can airtight sterilization, insulation, ventilative, the heat radiation of windowing again, before production, need to proving room sweep, dedusting, the sterilization of brush lime white, then the ozonization producing with trioxide disinfecting machine;
B, one grade fermemtation: sterilized culture material is pressed to 10% of gained biological potentiating agent gross weight, put into an indirect fermentation indoor, 3-4 ℃ to be chilled to, paves material, inoculation 4-5 part beneficial microorganism activator, abundant and culture material mixes, heap fermentation, piles thick 20-25cm, and heap temperature remains on 40-45 ℃, ferment 24 hours, obtain one grade fermemtation bacterium;
C, the fermentation of secondary enlarged culturing: by remaining sterilized culture material, put into another proving room of having sterilized, treat that material temperature is down to 45 ℃, material is paved to the one grade fermemtation bacterium of gained in inoculation step (5) B, fully mix, fermentation reactor system, piles thick 20-25cm, and heap temperature remains on 40-45 ℃, fermentation 16-24 hour, obtains biological potentiating agent;
(6) discharging, dry:
Biological potentiating agent is spread on the place of solarization instrument or surrounding ventilation, make thinner, stir, dry, dry;
(7) pack, store:
Dried biological potentiating agent, by quantitatively making small packages with plastics bag or doing to pack greatly with multiple film woven bag, is stored in health, cool place, dry, ventilation, wherein the living bacteria count of gained biological potentiating agent >=200,000,000/gram, miscellaneous bacteria rate≤15%, pH value is 5-7, validity period is not less than half a year.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110308064.4A CN102432352B (en) | 2011-10-12 | 2011-10-12 | Biological synergist |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110308064.4A CN102432352B (en) | 2011-10-12 | 2011-10-12 | Biological synergist |
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| CN102432352A CN102432352A (en) | 2012-05-02 |
| CN102432352B true CN102432352B (en) | 2014-04-16 |
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| CN104322597A (en) * | 2014-11-23 | 2015-02-04 | 青岛锐志农业技术服务有限公司 | Pesticide for preventing and controlling stored grain pests and preparation method thereof |
| CN104926537B (en) * | 2015-07-03 | 2018-06-19 | 东北农业大学 | A kind of corn seed manure specially containing double micro- ingredients |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1410397A (en) * | 2002-12-06 | 2003-04-16 | 赵鹏 | Biological organic and inorganic composite fertilizer and its production method |
| CN1490279A (en) * | 2003-08-21 | 2004-04-21 | 中国农业科学院油料作物研究所 | Fixed bacterial fertilizer and preparing method thereof |
| CN1834066A (en) * | 2006-04-04 | 2006-09-20 | 上海联业生物技术有限公司 | Biological orgamic fertilizer and prepn thereof |
| CN102134168A (en) * | 2010-01-26 | 2011-07-27 | 东莞市保得生物工程有限公司 | Microbial foliage synergist |
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2011
- 2011-10-12 CN CN201110308064.4A patent/CN102432352B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1410397A (en) * | 2002-12-06 | 2003-04-16 | 赵鹏 | Biological organic and inorganic composite fertilizer and its production method |
| CN1490279A (en) * | 2003-08-21 | 2004-04-21 | 中国农业科学院油料作物研究所 | Fixed bacterial fertilizer and preparing method thereof |
| CN1834066A (en) * | 2006-04-04 | 2006-09-20 | 上海联业生物技术有限公司 | Biological orgamic fertilizer and prepn thereof |
| CN102134168A (en) * | 2010-01-26 | 2011-07-27 | 东莞市保得生物工程有限公司 | Microbial foliage synergist |
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