CN1490279A - Fixed bacterial fertilizer and preparing method thereof - Google Patents
Fixed bacterial fertilizer and preparing method thereof Download PDFInfo
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- CN1490279A CN1490279A CNA031253075A CN03125307A CN1490279A CN 1490279 A CN1490279 A CN 1490279A CN A031253075 A CNA031253075 A CN A031253075A CN 03125307 A CN03125307 A CN 03125307A CN 1490279 A CN1490279 A CN 1490279A
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Abstract
An immobilized bacterial fertilizer is prepared from the sodium alginate, activated carbon, bacteria, CaCl2 and H2O. Its advantages are high resistance to ambient condition, great number of living bacteria, long storage time, and durable acting.
Description
Technical field
The present invention relates to a kind of microbial bacterial fertilizer.
Background technology
At present, there are 5 hang-ups in the production of microbial bacterial fertilizer and application, these problems are: 1. manufacture with wrapping process and must carry out under strict aseptic condition, carrying and the good condition of preservation process need, so production cost is high and be easy to pollution, and often bacterial manure becomes waste product in batch; 2. effectively preservation period is short, living bacteria count is few when using; 3. the bacterium number descended rapidly after the bacterial manure microorganism entered soil, was not easy to form advantage group, and bacterial manure effect validity period is short and effect is unstable; 4. need avoid agricultural measures such as agricultural chemicals, fertilizer when use in the field and be subjected to the restriction of environmental factor.5. southern high-temperature production and preserve the bacterial manure difficulty, autumn-sown crops such as rape are difficult to promote.
Summary of the invention
At above-mentioned deficiency, the purpose of this invention is to provide a kind of anti-envrionment conditions, living bacteria count height, can be in the room temperature prolonged preservation, use back little, thalline slowly-releasing affected by environment and at permanently effective immobilization bacterium fertilizer of growing surely of plant rhizosphere and preparation method thereof.
To achieve these goals, technical scheme of the present invention is: immobilization bacterium fertilizer is characterized in that: it is made up of sodium alginate, gac, bacterium thalline, U-Ramin MC, water, and the per-cent of the shared gross weight of each component is:
Sodium alginate 1.2-1.9, gac 10-16,
Bacterium thalline 0.7-1.2, U-Ramin MC 0.8-1.3
Water 81-86.
Described immobilization bacterium fertilizer, the optimized percentage of the shared gross weight of each component is:
Sodium alginate 1.57, gac 12.58,
Bacterium thalline 0.95, U-Ramin MC 1.05,
Water 83.85.
Described bacterium thalline is bacillus category (as Bacillus subtillis, Bacillus megatherium etc.) and nitrogen-fixing bacteria class (as rihizobium japonicum, peanut rhizobium, Bai Shi vinelandii etc.).
The preparation method of immobilization bacterium fertilizer is characterized in that: realized by following steps:
1). get sodium alginate and the water that weight percent is 1.2-1.9 and dissolve under 100-110 ℃ of temperature, making concentration is the 3.5-6.5% water solution A;
2). bacterium thalline and the water of getting weight percent and be 0.7-1.2 are made aqueous solution B, and the bacterium cell concentration is 2.0-4.0%;
3). getting weight percent is 0.8-1.3CaCl
2Make the aqueous solution C that concentration is 3.5-6.5% with water, stand-by;
4). weight percent for the 10-16 gac joins in the solution B, is shaken up; Add solution A again, shake up, become solution D;
5). the container that solution C will be housed is placed on the magnetic stirring apparatus and slowly stirs, and evenly drips solution D with dropper or peristaltic pump in container, and thalline is become particulate state by embedding gradually, forms immobilized cell particle, promptly gets the immobilization bacterium fertilizer of wet granular;
6). can use in the air-dry 1.5-2.5 of normal temperature days.
The invention solves 5 hang-ups that exist in present microbial bacteria fertilizer production and the application, these problems are: 1. manufacture with wrapping process and must carry out under strict aseptic condition, carrying and the good condition of preservation process need, so production cost is high and be easy to pollution, and often bacterial manure becomes waste product in batch; 2. effectively preservation period is short, living bacteria count is few when using; 3. the bacterium number descended rapidly after the bacterial manure microorganism entered soil, was not easy to form advantage group, and bacterial manure effect validity period is short and effect is unstable; 4. need avoid agricultural measures such as agricultural chemicals, fertilizer when use in the field and be subjected to the restriction of environmental factor.5. southern high-temperature production and preserve the bacterial manure difficulty, autumn-sown crops such as rape are difficult to promote.
With the sodium alginate is the embedding immobilization carrier of fixation of bacteria, gac is an absorption carrier, and U-Ramin MC is a fixing agent, and the three combines and adsorbs the bacterium thalline in a large number, and dry rapidly, play the effect that viable bacteria body, non-vulnerable to pollution environmental microorganism are preserved in rapid drying.Quick-drying thalline is in dormant state, not with external world's exchange material, but long-term surviving.The particulate state that forms has the effect of mechanical barrier, and protection bacterial manure bacterium avoids the influence of environment injurious factor, as the murder by poisoning of agricultural chemicals, protozoic eating and the antagonistic action of indigenous microorganism etc., can adapt to ecotope, forms group of local superiority.
The fixed particle has the effect that keeps bacterium, enters when meeting arid behind the soil, and bacterium continues dormancy and preserves; Meet and irrigate with when raining heavyly, particle can be avoided the bacterium leaching loss.After exsiccant fixation of bacteria particle enters soil, adsorption moisture continues to keep thalline, and discharges bacterium gradually in environment, plays the effect of slowly-releasing thalline, the high bacterium number that guarantee to continue and prolong the bacteria living time is in the rhizosphere sustainable development and form local superiority's monoid.
This immobilization bacterium fertilizer (hereinafter to be referred as bacterial manure) raw material is cheap, production process simply, does not need aseptic technique and packing, do not need specific conditions to preserve; The effective bacterium of this bacterial manure is counted height, genus bacillus can reach 10,000,000,000/more than the gram, the unit usage quantity reduces, and has reduced production and use cost; Can under the high temperature in 3 months summers of Wuhan, produce and preserve, solved the microbial-bacterial fertilizer production and the preservation problem of spring such as rape, wheat receipts summer sown crop.
Immobilization bacterium fertilizer is packed preservation with the exsiccant compound fertilizer granule, and mensuration bacterium number and validity are unaffected after 30 days.Be convenient to make composite bio-fertilizer.
The genus bacillus fertilizer of making of this method is deposited 60 days living bacteria counts and is surpassed 10 under Wuhan summer high-temperature room temperature in season
10Individual/gram, still had 10 in 90 days
9Individual/gram.And vermiculite, the peat composed of rotten mosses and organic solid that microbial fertilizer production is at present adopted and liquid bacterial fertilizer all are the bacterial manure of cultivating fundamental mode, and microorganism utilizes the breeding of nourishing and growing of carrier at the initial stage that enters carrier, reach just decline fast behind certain bacterium number.So the production program of existing bacterial manure will be decided according to the sowing time of plant, suitable product term is very short; Though and exist when dispatching from the factory the bacterium number high, the effective short problem of preservation period; The bacterial manure that is in vegetative activity phase and gnotobasis is pollution microbes very easily, low, the assorted bacterium rate height of living bacteria count when using to the field, and very difficult assurance reaches the general bacterial manure 10 of national requirements
8The standard of individual viable count.
Because particulate keeps and slow releasing function, after solidification bacterial fertilizer enters soil, progressively breeds at plant rhizosphere in preceding 10 days, the bacterium number reaches 7.2 * 10
9Individual/as more than the gram rhizosphere soil, still to surpass 1.9 * 10 in 30 days
8Individual/gram rhizosphere soil, surpass liquid bacterial fertilizer under the same terms more than 1000 times.Bacterium is measured in plant-growth after 3 months content still has 10
8Individual/gram rhizosphere soil, and the bacteria containing amount of inoculation liquid bacterial fertilizer is 10
2Individual/gram rhizosphere soil.During immobilization bacterium fertilizer placed into the soil, do not plant plant above, particle is held bacterium 10 after 3 months under field conditions (factors)
3Individual/gram rhizosphere soil.
With bacillus category and nitrogen-fixing bacteria class is the immobilization bacterium fertilizer that material is made, and the potted plant and field test through 3 years has proved above laboratory result.The result in 3 years is similar: initial stage bacterium number did not descend after bacterium entered soil, but rose gradually, reached the highlyest about 10 days, slowly descended afterwards, still had 10 to 3 months
8Individual/gram rhizosphere soil.The bacterial manure effect stability, volume increase in continuous 3 years is more than 11.9%, and the sclerotinia rot of colza preventive effect reaches 54.7%.
The present invention utilizes nontoxic, cheap sodium alginate to make embedding body, gac and calcium dioxide as absorption carrier and fixing agent, makes the new-type fertilizer of various genus bacillus and nitrogen-fixing bacteria, and suitable farm crop are cooked base fertilizer and fertilization strategy.Characteristics of the present invention are as follows: 1, the present invention hold very high effective bacterium number, genus bacillus can reach 10,000,000,000/more than the gram, surpass the living bacteria count of all bacterial manure at present.Room temperature storage contains the bacterium number and still remains on 10 after 3 months
10Individual/more than the gram.2, the anti-envrionment conditions of the present invention does not need aseptic technique and sterile packed, and production technique is easy, cost is low.3, the product of making can be preserved at normal temperatures, the available characteristic of high-and low-temperature resistance especially, be convenient to the spring gather in the autumn crops broadcast, the bio-feritlizer of crop such as autumn harvest spring sowing makes and uses.4, the immobilized cell fertilizer of making of present method can not have influence between the bacterial strain by suitable mixed in 2 weeks, be convenient to make and use composite microbic bacterial fertilizer.5, this kind fertilizer also can protect bacterium to avoid the infringement of soil microorganisms and injurious factor, and plays and hold the gentle effect that disengages bacterium of bacterium, and favourable bacterium is in the rhizosphere sustainable development and form local superiority, improves validity and extends the expiration date.
Description of drawings
Fig. 1 is wet granular figure of the present invention
Fig. 2 is the dried particle figure of the present invention
Embodiment
Embodiment 1:
Immobilization bacterium fertilizer, it is made up of sodium alginate, gac, bacterium thalline, U-Ramin MC, water, and the per-cent of the shared gross weight of each component is:
Embedding medium: sodium alginate 1.57,
Sorbent material: gac 12.58,
The bacterium thalline: Bacillus megatherium 0.95,
Fixing agent: U-Ramin MC 1.05,
Water 83.85.
The preparation method of immobilization bacterium fertilizer is characterized in that: realized by following steps:
1). getting weight percent and be 1.57 sodium alginate and weight percent is dissolving under 100-110 ℃ of temperature of 31.45 water, makes water solution A;
2). get weight percent and be 0.95 Bacillus megatherium and weight percent and be 31.45 water and make aqueous solution B;
3). getting weight percent is 1.05CaCl
2With weight percent be that 20.95 water is made aqueous solution C, stand-by;
4). with weight percent is that 12.58 gacs join in the solution B, shakes up; Add solution A again, shake up, become solution D;
5). the container that solution C will be housed is placed on the magnetic stirring apparatus and slowly stirs, and evenly drips solution D with dropper or peristaltic pump in container, and thalline is become particulate state by embedding gradually, forms immobilized cell particle, promptly gets the immobilization bacterium fertilizer of wet granular;
6). normal temperature air-dry (about 2 days) can use.
Embodiment 2:
Immobilization bacterium fertilizer, it is made up of sodium alginate, gac, bacterium thalline, U-Ramin MC, water, and the per-cent of the shared gross weight of each component is:
Embedding medium: sodium alginate 1.20,
Sorbent material: gac 11.3,
The bacterium thalline: Bacillus subtillis 0.7,
Fixing agent: U-Ramin MC 0.8,
Water 86.
The preparation method of immobilization bacterium fertilizer is characterized in that: realized by following steps:
1). getting weight percent and be 1.20 sodium alginate and weight percent is dissolving under 100-110 ℃ of temperature of 32.4 water, makes water solution A;
2). get weight percent and be 0.7 Bacillus subtillis and weight percent and be 32.4 water and make aqueous solution B;
3). getting weight percent is 0.8CaCl
2With weight percent be that 21.2 water is made aqueous solution C, stand-by;
4). with weight percent is that 11.3 gacs join in the solution B, shakes up; Add solution A again, shake up, become solution D;
5). the container that solution C will be housed is placed on the magnetic stirring apparatus and slowly stirs, and evenly drips solution D with dropper or peristaltic pump in container, and thalline is become particulate state by embedding gradually, forms immobilized cell particle, promptly gets the immobilization bacterium fertilizer of wet granular;
6). normal temperature can use in air-dry 2 days.
Embodiment 3:
Immobilization bacterium fertilizer, it is made up of sodium alginate, gac, bacterium thalline, U-Ramin MC, water, and the per-cent of the shared gross weight of each component is:
Embedding medium: sodium alginate 1.90,
Sorbent material: gac 14.6,
The bacterium thalline: rihizobium japonicum 1.2,
Fixing agent: U-Ramin MC 1.3,
Water 81.
The preparation method of immobilization bacterium fertilizer is characterized in that: realized by following steps:
1). getting weight percent and be 1.90 sodium alginate and weight percent is dissolving under 100-110 ℃ of temperature of 30.4 water, makes water solution A;
2). get weight percent and be 1.2 rihizobium japonicum and weight percent and be 30.4 water and make aqueous solution B;
3). getting weight percent is 1.3CaCl
2With weight percent be that 20.2 water is made aqueous solution C, stand-by;
4). with weight percent is that 14.6 gacs join in the solution B, shakes up; Add solution A again, shake up, become solution D;
5). the container that solution C will be housed is placed on the magnetic stirring apparatus and slowly stirs, and evenly drips solution D with dropper or peristaltic pump in container, and thalline is become particulate state by embedding gradually, forms immobilized cell particle, promptly gets the immobilization bacterium fertilizer of wet granular;
6). normal temperature can use in air-dry 1.5 days.
Embodiment 4:
Immobilization bacterium fertilizer, it is made up of sodium alginate, gac, bacterium thalline, U-Ramin MC, water, and the per-cent of the shared gross weight of each component is:
Embedding medium: sodium alginate 1.2,
Sorbent material: gac 16,
The bacterium thalline: Bai Shi vinelandii 1.0,
Fixing agent: U-Ramin MC 0.8,
Water 81.
The preparation method of immobilization bacterium fertilizer is characterized in that: realized by following steps:
1). getting weight percent and be 1.2 sodium alginate and weight percent is dissolving under 100-110 ℃ of temperature of 31.4 water, makes water solution A;
2). get weight percent and be 1.0 Bai Shi vinelandii and weight percent and be 31.4 water and make aqueous solution B;
3). getting weight percent is 0.8CaCl
2With weight percent be that 18.2 water is made aqueous solution C, stand-by;
4). with weight percent is that 16 gacs join in the solution B, shakes up; Add solution A again, shake up, become solution D;
5). the container that solution C will be housed is placed on the magnetic stirring apparatus and slowly stirs, and evenly drips solution D with dropper or peristaltic pump in container, and thalline is become particulate state by embedding gradually, forms immobilized cell particle, promptly gets the immobilization bacterium fertilizer of wet granular;
6). normal temperature can use in air-dry 2.5 days.
Claims (4)
1. immobilization bacterium fertilizer, it is characterized in that: it is made up of sodium alginate, gac, bacterium thalline, U-Ramin MC, water, and the per-cent of the shared gross weight of each component is:
Sodium alginate 1.2-1.9, gac 10-16,
Bacterium thalline 0.7-1.2, U-Ramin MC 0.8-1.3
Water 81-86.
2. immobilization bacterium fertilizer according to claim 1 is characterized in that: described immobilization bacterium fertilizer, and the optimized percentage of the shared gross weight of each component is:
Sodium alginate 1.57, gac 12.58,
Bacterium thalline 0.95, U-Ramin MC 1.05,
Water 83.85.
3. immobilization bacterium fertilizer according to claim 1 and 2 is characterized in that: described bacterium thalline is bacillus category or nitrogen-fixing bacteria class.
4. the preparation method of immobilization bacterium fertilizer is characterized in that: realized by following steps:
1). get sodium alginate and the water that weight percent is 1.2-1.9 and dissolve under 100-110 ℃ of temperature, making concentration is the 3.5-6.5% water solution A;
2). bacterium thalline and the water of getting weight percent and be 0.7-1.2 are made aqueous solution B, and the bacterium cell concentration is 2.0-4.0%;
3). getting weight percent is 0.8-1.3CaCl
2Make the aqueous solution C that concentration is 3.5-6.5% with water, stand-by;
4). weight percent for the 10-16 gac joins in the solution B, is shaken up; Add solution A again, shake up, become solution D;
5). the container that solution C will be housed is placed on the magnetic stirring apparatus and slowly stirs, and evenly drips solution D with dropper or peristaltic pump in container, and thalline is become particulate state by embedding gradually, forms immobilized cell particle, promptly gets the immobilization bacterium fertilizer of wet granular.
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| CN 03125307 CN1216014C (en) | 2003-08-21 | 2003-08-21 | Fixed bacterial fertilizer and preparing method thereof |
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| CN 03125307 CN1216014C (en) | 2003-08-21 | 2003-08-21 | Fixed bacterial fertilizer and preparing method thereof |
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| CN101519641B (en) * | 2009-04-03 | 2011-07-20 | 南京师范大学 | Method for producing mixed microorganism inoculum |
| CN102391042A (en) * | 2011-08-03 | 2012-03-28 | 青岛丰泰海洋生物科技有限公司 | Production method of seaweed biological liquid fertilizer |
| CN102409035A (en) * | 2011-05-06 | 2012-04-11 | 江南大学 | Preparation method and application of microecological bactericide for sustained release in water |
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| CN106754500A (en) * | 2016-12-14 | 2017-05-31 | 黑龙江省科学院大庆分院 | The preparation method and applications of alfalfa nodule bacteria research immobilization embedded particles formulation |
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2003
- 2003-08-21 CN CN 03125307 patent/CN1216014C/en not_active Expired - Fee Related
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| CN101519632B (en) * | 2009-04-01 | 2013-07-17 | 天津大学 | Device and technology for preparing engineering embedding fixing microorganism spheric bodies |
| CN101519641B (en) * | 2009-04-03 | 2011-07-20 | 南京师范大学 | Method for producing mixed microorganism inoculum |
| CN102409035A (en) * | 2011-05-06 | 2012-04-11 | 江南大学 | Preparation method and application of microecological bactericide for sustained release in water |
| CN102391042A (en) * | 2011-08-03 | 2012-03-28 | 青岛丰泰海洋生物科技有限公司 | Production method of seaweed biological liquid fertilizer |
| CN102432352A (en) * | 2011-10-12 | 2012-05-02 | 四川宏塔生物有机肥有限公司 | Biological synergist |
| CN102432352B (en) * | 2011-10-12 | 2014-04-16 | 四川宏塔生物有机肥有限公司 | Biological synergist |
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| CN102649655B (en) * | 2012-04-27 | 2015-04-01 | 中国科学院上海高等研究院 | Carbon-based compound fertilizer containing probiotic organisms and preparation method of carbon-based compound fertilizer |
| CN103979534A (en) * | 2014-05-14 | 2014-08-13 | 石家庄金太阳生物有机肥有限公司 | Active carbon preparing method by adopting walnut shell as raw material and application of the active carbon |
| CN105087541A (en) * | 2015-09-07 | 2015-11-25 | 博天环境集团股份有限公司 | Microbe immobilizing method |
| CN106754500A (en) * | 2016-12-14 | 2017-05-31 | 黑龙江省科学院大庆分院 | The preparation method and applications of alfalfa nodule bacteria research immobilization embedded particles formulation |
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| CN118239807A (en) * | 2024-03-25 | 2024-06-25 | 湖南昇阳富硒生物科技有限公司 | Preparation method of embedded selenium-enriched composite microbial fertilizer |
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