CN102428190B - 用于检测等位基因变体的方法、组合物和试剂盒 - Google Patents

用于检测等位基因变体的方法、组合物和试剂盒 Download PDF

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CN102428190B
CN102428190B CN201080021402.XA CN201080021402A CN102428190B CN 102428190 B CN102428190 B CN 102428190B CN 201080021402 A CN201080021402 A CN 201080021402A CN 102428190 B CN102428190 B CN 102428190B
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allele
specific
base
specific primers
pcr
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CN102428190A (zh
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C·陈
R·谭
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Life Technologies Corp
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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CN201080021402.XA 2009-03-27 2010-03-26 用于检测等位基因变体的方法、组合物和试剂盒 Active CN102428190B (zh)

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US16423009P 2009-03-27 2009-03-27
US61/164,230 2009-03-27
US18677509P 2009-06-12 2009-06-12
US61/186,775 2009-06-12
US25162309P 2009-10-14 2009-10-14
US61/251,623 2009-10-14
US25350109P 2009-10-20 2009-10-20
US61/253,501 2009-10-20
US25858209P 2009-11-05 2009-11-05
US61/258,582 2009-11-05
US12/641,321 2009-12-17
US12/641,321 US9534255B2 (en) 2008-12-17 2009-12-17 Methods, compositions, and kits for detecting allelic variants
PCT/US2010/028963 WO2010111682A2 (en) 2009-03-27 2010-03-26 Methods, compositions, and kits for detecting allelic variants

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Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9534255B2 (en) * 2008-12-17 2017-01-03 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
CN102428190B (zh) 2009-03-27 2014-02-26 生命技术公司 用于检测等位基因变体的方法、组合物和试剂盒
US8815515B1 (en) 2009-09-24 2014-08-26 University Of Utah Research Foundation Methods, compositions, and kits for rare allele detection
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US8825412B2 (en) 2010-05-18 2014-09-02 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
GB201100150D0 (en) 2011-01-06 2011-02-23 Epistem Ltd Mutational analysis
CA2824387C (en) 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
KR20210056458A (ko) * 2011-05-04 2021-05-18 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
EP2744916A4 (en) 2011-07-13 2015-06-17 Primeradx Inc MULTIMODAL PROCEDURE FOR THE SIMULTANEOUS DETECTION AND QUANTIFICATION OF MULTIPLE NUCLEIC ACIDS IN ONE SAMPLE
WO2013026027A1 (en) * 2011-08-18 2013-02-21 Nestec S.A. Compositions and methods for detecting allelic variants
US9260714B2 (en) * 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
EP2607495A1 (en) * 2011-12-23 2013-06-26 Genomica S.A.U. Method for detection of KRAS mutations
AU2013221642A1 (en) * 2012-02-15 2014-09-25 Janssen Diagnostics, Llc Highly sensitive method for detecting low frequency mutations
WO2013158215A1 (en) 2012-04-16 2013-10-24 Life Technologies Corporation Oligonucleotides and methods for the preparationof rna libraries
EP2841598B1 (en) 2012-04-25 2018-03-14 Ho, Tho Huu Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
CN103114146B (zh) * 2013-02-28 2015-03-11 张戈 一种检测K-ras基因突变的试剂盒及其检测方法
WO2014164874A2 (en) * 2013-03-13 2014-10-09 Becton, Dickinson And Company Methods and compositions for modulation of amplification efficiency
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物
KR102126031B1 (ko) * 2013-07-23 2020-07-08 삼성전자주식회사 유전적 변이를 함유하는 핵산 검출 방법
EP3083960B1 (en) * 2013-12-16 2021-02-03 Syddansk Universitet Ras exon 2 skipping for cancer treatment
EP3957749A1 (en) 2014-04-21 2022-02-23 Natera, Inc. Detecting tumour specific mutations in biopsies with whole exome sequencing and in cell-free samples
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
PH12016502542B1 (en) * 2014-05-19 2024-01-17 Univ Rice William M Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
CN107148481A (zh) * 2014-07-14 2017-09-08 苏黎世大学医学与自然科学院 用于确定患有braf‑阳性癌症的患者为braf抑制剂非响应者且为mapk/erk抑制剂响应者的工具和方法
CN105671137A (zh) * 2014-11-17 2016-06-15 武汉白原科技有限公司 运动心率反应的检测试剂盒和计算方法
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
CN104862401B (zh) * 2015-05-29 2017-10-31 沈阳优吉诺生物科技有限公司 检测kras基因热点突变位点的引物、试剂盒及其pcr方法
US20170327868A1 (en) * 2015-10-21 2017-11-16 Admera Health LLC Blocker based enrichment system and uses thereof
CN108291253A (zh) * 2015-11-25 2018-07-17 综合基因技术公司 用于变体检测的方法
JP7280044B2 (ja) 2016-04-15 2023-05-23 ナテラ， インコーポレイテッド 肺癌の検出方法
US11414686B2 (en) 2016-05-06 2022-08-16 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
JP7279633B2 (ja) * 2017-03-29 2023-05-23 東洋紡株式会社 核酸の検出方法
CN109306374A (zh) * 2017-09-08 2019-02-05 广州健天基因技术有限公司 用于检测人类pik3ca基因e545k突变的引物、检测方法及试剂盒
US12131803B2 (en) 2017-10-17 2024-10-29 The Broad Institute, Inc. Methods and systems for detection of somatic structural variants
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
EP3759118A4 (en) 2018-02-20 2022-03-23 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
EP3766992A4 (en) * 2018-03-15 2021-12-01 Eiken Kagaku Kabushiki Kaisha OLIGONUCLEOTIDE PROBE FOR DETECTION OF MONONUCLEOTIDIC POLYMORPHISM, AND PROCESS FOR DIFFERENTIATION OF CIS FORM AND TRANS FORM
US12024738B2 (en) 2018-04-14 2024-07-02 Natera, Inc. Methods for cancer detection and monitoring
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN110106063B (zh) * 2019-05-06 2022-07-08 臻和精准医学检验实验室无锡有限公司 基于二代测序的用于神经胶质瘤1p/19q联合缺失检测的系统
WO2020247263A1 (en) 2019-06-06 2020-12-10 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
CN114555829A (zh) * 2019-10-02 2022-05-27 新泽西鲁特格斯州立大学 检测稀有序列变体的测定方法和试剂盒
CN111607652B (zh) * 2020-05-29 2022-02-11 中国科学院南海海洋研究所 一种与马氏珠母贝生长性状关联的InDel标记、检测引物组及开发方法
CN114645077B (zh) * 2020-12-17 2025-06-03 厦门大学 一种检测受体样品中供体的存在或比例的方法和试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070184457A1 (en) * 2004-08-02 2007-08-09 Genevieve Pont-Kingdon Method for long range allele-specific PCR

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1008183A (en) 1911-07-17 1911-11-07 P & M Co Rail-anchor.
US5585481A (en) * 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
EP0472648A4 (en) 1989-05-18 1992-09-16 Microprobe Corporation Crosslinking oligonucleotides
US5451463A (en) * 1989-08-28 1995-09-19 Clontech Laboratories, Inc. Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5419966A (en) * 1991-06-10 1995-05-30 Microprobe Corporation Solid support for synthesis of 3'-tailed oligonucleotides
WO1993001315A1 (en) * 1991-07-01 1993-01-21 The Blood Center Of Southeastern Wisconsin PEN POLYMORPHISM OF HUMAN PLATELET MEMBRANE GLYCOPROTEIN IIIa AND DIAGNOSTIC AND THERAPEUTIC APPLICATION THEREOF
US5512677A (en) 1991-08-13 1996-04-30 National Science Council 3-substituted methyl-2,3-dihydroimidazo 1,2-c!quinazoline derivatives, the preparation and use thereof
GB9211979D0 (en) * 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5767259A (en) 1994-12-27 1998-06-16 Naxcor Oligonucleotides containing base-free linking groups with photoactivatable side chains
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
ATE198511T1 (de) * 1994-04-29 2001-01-15 Perkin Elmer Corp Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation
US5512441A (en) * 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method
US5801155A (en) * 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6312894B1 (en) * 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5736626A (en) * 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
US6852487B1 (en) * 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
CA2252048C (en) * 1996-04-12 2008-03-11 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
WO1997045559A1 (en) * 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6043070A (en) 1997-08-29 2000-03-28 The Perkin-Elmer Corporation Phosphoramidate-phosphodiester oligonucleotide chimera as primers
AU1366299A (en) * 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6485901B1 (en) * 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6303305B1 (en) * 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
US6383752B1 (en) 1999-03-31 2002-05-07 Hybridon, Inc. Pseudo-cyclic oligonucleobases
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US6727356B1 (en) * 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US7495070B2 (en) * 2000-04-24 2009-02-24 Yale University Protein binding miniature proteins
US6783934B1 (en) * 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
CN1293204C (zh) * 2000-08-30 2007-01-03 戴诺生物技术有限公司 等位基因的测定方法
US20030082549A1 (en) * 2000-08-30 2003-05-01 Xiangjun Liu Method for determining alleles
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
DE10112515B4 (de) 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
JP4502168B2 (ja) * 2001-07-06 2010-07-14 ルネサスエレクトロニクス株式会社 化学機械研磨装置および化学機械研磨方法
AU2002334307A1 (en) 2001-09-04 2003-03-18 Exiqon A/S Novel lna compositions and uses thereof
US6593091B2 (en) * 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US6589250B2 (en) * 2001-11-20 2003-07-08 Stephen A. Schendel Maxillary distraction device
US7253007B2 (en) * 2001-11-30 2007-08-07 Chemocentryx, Inc. Compositions and methods for detecting and treating diseases and conditions related to chemokine receptors
WO2003050304A1 (en) * 2001-12-08 2003-06-19 Seegene, Inc Annealing control primer system for regulating primer annealing specificity and its applications
ES2314093T3 (es) 2001-12-08 2009-03-16 Seegene, Inc. Cebador para el control de la reasociacion y sus usos.
US7228237B2 (en) * 2002-02-07 2007-06-05 Applera Corporation Automatic threshold setting and baseline determination for real-time PCR
US6818420B2 (en) * 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
EP3000899A1 (en) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplex amplification of polynucleotides
US7348146B2 (en) 2003-10-02 2008-03-25 Epoch Biosciences, Inc. Single nucleotide polymorphism analysis of highly polymorphic target sequences
WO2005098035A2 (en) 2004-04-06 2005-10-20 Epigenomics Ag Method for the quantification of methylated dna
US7517978B1 (en) 2004-04-14 2009-04-14 Applied Biosystems, Llc Modified oligonucleotides and applications thereof
DE102005011398A1 (de) * 2005-03-03 2006-09-14 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA
EP2477029A1 (en) 2005-06-02 2012-07-18 Fluidigm Corporation Analysis using microfluidic partitioning devices
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US9797005B2 (en) * 2005-11-23 2017-10-24 University Of Southern California High throughput method of DNA methylation haplotyping
WO2007106534A2 (en) * 2006-03-14 2007-09-20 Harbor-Ucla Research And Education Institute Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
WO2008111990A1 (en) * 2006-06-14 2008-09-18 Cellpoint Diagnostics, Inc. Rare cell analysis using sample splitting and dna tags
EP1905842A1 (en) 2006-09-26 2008-04-02 DiaSorin S.p.A. Method for detection of mutant alleles combining Real Time PCR and REMS-PCR
GB0703996D0 (en) 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
US20090053719A1 (en) * 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
WO2009135093A2 (en) 2008-04-30 2009-11-05 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers
US20100041048A1 (en) 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
US9534255B2 (en) * 2008-12-17 2017-01-03 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
US20110287424A1 (en) * 2009-03-27 2011-11-24 Life Technologies Corporation Methylation-specific competitive allele-specific taqman polymerase chain reaction (cast-pcr)
CN102428190B (zh) * 2009-03-27 2014-02-26 生命技术公司 用于检测等位基因变体的方法、组合物和试剂盒
DK2576832T3 (en) 2010-05-25 2016-06-20 Univ Johns Hopkins CONFIGURATIONS AND METHODS FOR DETECTION OF neoplasia
EP2400035A1 (en) 2010-06-28 2011-12-28 Technische Universität München Methods and compositions for diagnosing gastrointestinal stromal tumors
US20120214160A1 (en) * 2011-01-14 2012-08-23 Life Technologies Corporation Methods, compositions, and kits for detecting rare cells
KR20210056458A (ko) 2011-05-04 2021-05-18 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
WO2014004726A1 (en) 2012-06-26 2014-01-03 Caifu Chen Methods, compositions and kits for the diagnosis, prognosis and monitoring of cancer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070184457A1 (en) * 2004-08-02 2007-08-09 Genevieve Pont-Kingdon Method for long range allele-specific PCR

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
3"-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures;Igor V. Kutyavin et al;《nucleic acids research》;20000115;第28卷(第2期);摘要，655-656页 *
Mutation Detection by Real-Time PCR: A Simple, Robust and Highly Selective Method;MORLAN I.V. et al;《PLOS ONE》;20090225;第4卷;摘要，Introduction，Materials and Methods部分 *
萨姆布鲁克.第8章.《分子克隆实验指南 第三版》.2002, *

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