CN102388794B - Medicinal Chrysanthemum morifolium Ramat cuttage breeding method - Google Patents

Medicinal Chrysanthemum morifolium Ramat cuttage breeding method Download PDF

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CN102388794B
CN102388794B CN2011102206664A CN201110220666A CN102388794B CN 102388794 B CN102388794 B CN 102388794B CN 2011102206664 A CN2011102206664 A CN 2011102206664A CN 201110220666 A CN201110220666 A CN 201110220666A CN 102388794 B CN102388794 B CN 102388794B
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cuttage
chrysanthemum morifolium
plugged ear
rooting
morifolium ramat
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CN102388794A (en
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郭巧生
毛鹏飞
汪涛
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Nanjing Agricultural University
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Nanjing Agricultural University
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Abstract

The invention provides a medicinal Chrysanthemum morifolium Ramat cuttage breeding method, which can rapidly promote rooting, increase roots and promote root extension. The method is of great importance to the improvement of the quality of medicinal Chrysanthemum morifolium Ramat seedlings, the realization of the rapid breeding of the medicinal Chrysanthemum morifolium Ramat and the promotion of the standardized cultivation of the medicinal Chrysanthemum morifolium Ramat.

Description

A kind of method of Chrysanthemum morifolium cottage propagation
Technical field
The present invention relates to a kind of method of Chrysanthemum morifolium cuttage, belong to traditional Chinese medicine culture technique field.
Background technology
Chrysanthemum Chrysanthemum morifolium Ramat. is composite family Chrysanthemum herbaceos perennial, its dry capitulum is parts of generic medicinal plants, for " the contained kind of Chinese pharmacopoeia (version in 2010), ' red heart chrysanthemum ' wherein is a good cultivar of " Chrysanthemum morifolium " in the application.Have loose wind heat-clearing, flat liver makes eye bright, and is clearing heat and detoxicating, is used for anemopyretic cold, have a headache dizzy, and red eye, swell pain, eyes is dim-sighted, the effect of sore and toxic.Because the Chrysanthemum morifolium market demand increases gradually, area under cultivation constantly enlarges, and the seedling demand is large.The propagation method of Chrysanthemum morifolium has plant division and cottage propagation, due to plant division and the propagation by layering reproduction coefficient lower, branch stem cripetura and branch is many, flower is less, and that cottage propagation not only has is simple, keep the advantages such as merit of kind, and can overcome the deficiencies such as the seedling that plant division and propagation by layering causes is few, production declining, can also prune in conjunction with flower and carry out cuttage, the needs that seedling is produced can be reached, cost can be saved again.
There is no relevant report about the Chrysanthemum morifolium cottage method, view and admire chrysanthemum cottage method report more, compare with viewing and admiring the chrysanthemum cottage method, method provided by the present invention comparatively comprehensively and system, the somatotropin of comparative selection and matrix are more comprehensive, are more suitable for the cuttage in Chrysanthemum morifolium.
The invention provides a kind of method of Chrysanthemum morifolium cottage propagation, can promote fast it to take root, increase the number of taking root, promote the root elongation.For improving the Chrysanthemum morifolium seedling quality, realize the Fast-propagation of Chrysanthemum morifolium, promote that the Chrysanthemum morifolium standardized cultivation is significant.
Summary of the invention
Traditional Chrysanthemum morifolium cottage method is disunity and standard still, and cutting position, cutting medium and HORMONE TREATMENT etc. there is no uniform requirement, cause cuttage seedling quality level uneven.
The object of the present invention is to provide a kind of method of effective promotion Chrysanthemum morifolium cuttage root-taking, make its number of taking root bring up to 56 from common 17, root length significantly improves to more than 6.35cm from the common 3.02cm of germination rate.
The method of Chrysanthemum morifolium cottage propagation provided by the present invention comprises:
A. the selection of plugged ear: choose stem end spray and stem section as the plugged ear of cuttage, wherein the root system efficiency index of stem end spray is 3.13, is significantly higher than 1.4 of stem section.
B. the Hormones pretreatment of plugged ear: plugged ear is placed in the different solutions that heteroauxin (IAA), methyl α-naphthyl acetate (NAA), indolebutyric acid IBA be configured to respectively 250mg/L, 500mg/L, 750mg/L, 1000mg/L soaks 10min, wherein the IBA rooting efficiency of 750mg/L is best, has reached 17.68.
1), river sand C. the configuration of cutting medium: plugged ear is inserted in river sand, vermiculite, perlite, peat, river sand: vermiculite: perlite: peat (1: 1: 1:: vermiculite: perlite (2: 1: 1), peat: vermiculite (1: 1) and peat: in 8 kinds of different matrix of perlite (1: 1), be mixedly configured into river sand, vermiculite, perlite, peat wherein that (1: 1: 1: mixed-matrix rooting efficiency 1) was best, and the root system efficiency index has reached 8.84.
D. the cuttage of plugged ear: will through the plugged ear cuttage after Hormones pretreatment in matrix, irrigate and sterilize.
E. seedling management: will carry out disinfection after cuttage, and in time water and keep the skin wet.
The invention has the advantages that:
Chrysanthemum morifolium cuttage breeding method provided by the invention can significantly improve the Chrysanthemum morifolium seedling quality, realizes the Fast-propagation of Chrysanthemum morifolium, promotes that the Chrysanthemum morifolium standardized cultivation is significant.
Description of drawings
Fig. 1 is different, and the cuttage rooting effects compare, the tender tip cuttage of left stem end, right stem segment cuttage
Fig. 2 cuttage blank condition of rooting
Fig. 3 IAA250mg/L processes condition of rooting
Fig. 4 IAA 500mg/L processes condition of rooting
Fig. 5 IAA750mg/L processes condition of rooting
Fig. 6 IAA 1000mg/L processes condition of rooting
Fig. 7 IBA250mg/L processes condition of rooting
Fig. 8 IBA 500mg/L processes condition of rooting
Fig. 9 IBA750mg/L processes condition of rooting
Figure 10 IBA 1000mg/L processes condition of rooting
Figure 11 NAA250mg/L processes condition of rooting
Figure 12 NAA500mg/L processes condition of rooting
Figure 13 NAA 750mg/L processes condition of rooting
Figure 14 NAA 1000mg/L processes condition of rooting
Embodiment
Test example 1 Chrysanthemum morifolium cottage propagation test
One, the selection of plugged ear: choose stem end spray and stem section as the plugged ear of cuttage, insert in matrix.Result of the test sees as table 1, and is best with tender tip cuttage effect as can be seen from the table, and the number of taking root reaches 13, and root is long average 6.98, and the root system efficiency index is 3.13, is significantly higher than the stem segment cuttage.
The impact of the different cuttages of table 1 position on cuttage root-taking
Figure BSA00000549821000031
Two, the Hormones pretreatment of plugged ear: plugged ear is placed in the different solutions that heteroauxin (IAA), methyl α-naphthyl acetate (NAA), indolebutyric acid IBA be configured to respectively 250mg/L, 500mg/L, 750mg/L, 1000mg/L soaks 10min, result of the test sees Table 2.The hormone-treated root system successful of process is higher than the blank of not processing as can be seen from the table, wherein easily process rooting efficiency with the IBA of 750mg/L best, the number of on average taking root is 56, the long average out to 6.35 of root, the root system effect is 17.68, significantly higher than other processing.
The impact on cuttage root-taking of table 2 hormon and solubility thereof
Figure BSA00000549821000032
1), river sand three, the configuration of cutting medium: plugged ear is inserted in river sand, vermiculite, perlite, peat, river sand: vermiculite: perlite: (1: 1: 1:: vermiculite: perlite (2: 1: 1), peat: vermiculite (1: 1) and peat: in 8 kinds of different matrix of perlite (1: 1), result of the test was as shown in table 3 for peat.As can be seen from the table with river sand: vermiculite: perlite: (1: 1: 1: mixed-matrix rooting efficiency 1) was best, was significantly higher than other matrix cuttages for peat.
The impact of table 3 different substrates on cuttage root-taking
Figure BSA00000549821000033
Four, the cuttage of plugged ear: will through the plugged ear cuttage after Hormones pretreatment in matrix, irrigate and sterilize.
Five, seedling management: will carry out disinfection after cuttage, and in time water and keep the skin wet.
Specific embodiment 1 promotes the method for Chrysanthemum morifolium cuttage root-taking
A. the selection of plugged ear: choose stem end spray and stem section as the plugged ear of cuttage, wherein the root system efficiency index of stem end spray is 3.13, is significantly higher than 1.4 of stem section.
B. the Hormones pretreatment of plugged ear: plugged ear is placed in the different solutions that heteroauxin (IAA), methyl α-naphthyl acetate (NAA), indolebutyric acid IBA be configured to respectively 250mg/L, 500mg/L, 750mg/L, 1000mg/L soaks 10min, wherein the IBA rooting efficiency of 750mg/L is best, has reached 17.68.
1), river sand C. the configuration of cutting medium: plugged ear is inserted in river sand, vermiculite, perlite, peat, river sand: vermiculite: perlite: peat (1: 1: 1:: vermiculite: perlite (2: 1: 1), peat: vermiculite (1: 1) and peat: in 8 kinds of different matrix of perlite (1: 1), be mixedly configured into river sand, vermiculite, perlite, peat wherein that (1: 1: 1: mixed-matrix rooting efficiency 1) was best, and the root system efficiency index has reached 8.84.
D. the cuttage of plugged ear: will through the plugged ear cuttage after Hormones pretreatment in matrix, irrigate and sterilize.
E. seedling management: will carry out disinfection after cuttage, and in time water and keep the skin wet.

Claims (1)

1. the method for a Chrysanthemum morifolium Chrysanthemum morifolium Ramat. red heart chrysanthemum cottage propagation comprises:
A. the selection of plugged ear: the stem end spray of clip 8~10cm length is as the plugged ear of cuttage;
B. the Hormones pretreatment of plugged ear: the IBA solution that plugged ear is placed in 750mg/L soaks 10min;
C. the configuration of cutting medium: it is 1: 1: 1 that river sand, vermiculite, perlite, peat are hybridly prepared into weight ratio: 1 mixed-matrix;
D. the cuttage of plugged ear: will through the plugged ear cuttage after Hormones pretreatment in mixed-matrix, irrigate and sterilize;
E. seedling management: will carry out disinfection after cuttage, and in time water and keep the skin wet.
CN2011102206664A 2011-08-03 2011-08-03 Medicinal Chrysanthemum morifolium Ramat cuttage breeding method Expired - Fee Related CN102388794B (en)

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CN102792830B (en) * 2011-05-24 2014-04-16 江苏骏马农林科技股份有限公司 Time-saving and labour-saving cultivation method for chrysanthemum cut flower
CN102754560B (en) * 2012-08-06 2013-10-02 安徽菊泰滁菊草本科技有限公司 Method for high-quality chuzhou chrysanthemum hole tray cuttage seedling culture
CN103493717A (en) * 2013-08-29 2014-01-08 合肥市潜溪山庄农业生态园有限公司 Chrysanthemum soilless culture medium and preparation method thereof
CN103875380B (en) * 2013-12-26 2016-01-20 云南丰岛花卉有限公司 A kind of Cut Flower Chrysanthemum Morifolium seedling breeding, cultivation method
CN104472249A (en) * 2014-11-26 2015-04-01 中南林业科技大学 Inducing and domestication combined optimizing method for hydroponics flower root system
CN104756840B (en) * 2015-03-09 2017-10-03 嘉兴欣盛园林绿化景观工程有限公司 The cuttage technique and its breeding apparatus of a kind of round end Distylium racemosum
CN105493867A (en) * 2015-12-25 2016-04-20 南京农业大学 Technology for continuously culturing chrysanthemum morifolium ramat and prunella vulgaris in Subei coastal areas
CN106376321A (en) * 2016-08-30 2017-02-08 成都苗夫现代苗木科技有限公司 Echinacea purpurea cutting breeding method
CN109362371A (en) * 2018-12-25 2019-02-22 禹州市锦绣大地生态农业有限公司 A kind of method of golden chrysanthemum cuttage
CN109906788A (en) * 2019-05-06 2019-06-21 江苏润顺生态农业科技有限公司 A kind of Chrysanthemum morifolium intensive seedling production method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1430873A (en) * 2003-01-23 2003-07-23 南京农业大学 Method for breeding officinal white chrysantheman in species of white chrysanthemam in red heat
CN101647352A (en) * 2008-08-15 2010-02-17 王安合 Method for cultivating chrysanthemums

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1430873A (en) * 2003-01-23 2003-07-23 南京农业大学 Method for breeding officinal white chrysantheman in species of white chrysanthemam in red heat
CN101647352A (en) * 2008-08-15 2010-02-17 王安合 Method for cultivating chrysanthemums

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
不同基质对菊花扦插效果的影响;项玉英 等;《上海农业科技》;20061031(第5期);第138页 *
施忠辉.黄山贡菊花栽培及加工.《特种经济动植物》.1999,第 卷(第6期),26-27.
李凤玲.菊花嫩枝扦插繁殖新技术.《农村农业农民》.2004,第 卷(第1期),30-31.
菊花嫩枝扦插繁殖新技术;李凤玲;《农村农业农民》;20040131;第 卷(第1期);第30-31页 *
菊花扦插生根研究进展;黄熊娟 等;《广西农业科学》;20081031;第39卷(第5期);第668-671页 *
项玉英 等.不同基质对菊花扦插效果的影响.《上海农业科技》.2006,(第5期),138.
黄山贡菊花栽培及加工;施忠辉;《特种经济动植物》;19990630;第 卷(第6期);第26-27页 *
黄熊娟 等.菊花扦插生根研究进展.《广西农业科学》.2008,第39卷(第5期),668-671.

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