CN102388136B - 新颖的核重编程序物质 - Google Patents

新颖的核重编程序物质 Download PDF

Info

Publication number
CN102388136B
CN102388136B CN201080011401.7A CN201080011401A CN102388136B CN 102388136 B CN102388136 B CN 102388136B CN 201080011401 A CN201080011401 A CN 201080011401A CN 102388136 B CN102388136 B CN 102388136B
Authority
CN
China
Prior art keywords
cells
myc
sox2
oct3
ips
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201080011401.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN102388136A (zh
Inventor
山中伸弥
五岛直树
前川桃子
河村义史
望月宏美
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
JAPAN BIOLOG INFORMATICS CONSO
National Institute of Advanced Industrial Science and Technology AIST
Kyoto University NUC
Original Assignee
JAPAN BIOLOG INFORMATICS CONSO
National Institute of Advanced Industrial Science and Technology AIST
Kyoto University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by JAPAN BIOLOG INFORMATICS CONSO, National Institute of Advanced Industrial Science and Technology AIST, Kyoto University NUC filed Critical JAPAN BIOLOG INFORMATICS CONSO
Publication of CN102388136A publication Critical patent/CN102388136A/zh
Application granted granted Critical
Publication of CN102388136B publication Critical patent/CN102388136B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201080011401.7A 2009-02-27 2010-02-19 新颖的核重编程序物质 Active CN102388136B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US20885309P 2009-02-27 2009-02-27
US61/208853 2009-02-27
US61/208,853 2009-02-27
US27612309P 2009-09-08 2009-09-08
US61/276,123 2009-09-08
US61/276123 2009-09-08
PCT/JP2010/053024 WO2010098419A1 (en) 2009-02-27 2010-02-19 Novel nuclear reprogramming substance

Publications (2)

Publication Number Publication Date
CN102388136A CN102388136A (zh) 2012-03-21
CN102388136B true CN102388136B (zh) 2014-10-01

Family

ID=42665623

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080011401.7A Active CN102388136B (zh) 2009-02-27 2010-02-19 新颖的核重编程序物质

Country Status (9)

Country Link
US (1) US8951801B2 (OSRAM)
EP (2) EP2607476B1 (OSRAM)
JP (1) JP5553289B2 (OSRAM)
KR (1) KR101764100B1 (OSRAM)
CN (1) CN102388136B (OSRAM)
CA (1) CA2753845C (OSRAM)
IL (1) IL214833A (OSRAM)
SG (1) SG173876A1 (OSRAM)
WO (1) WO2010098419A1 (OSRAM)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010098419A1 (en) 2009-02-27 2010-09-02 Kyoto University Novel nuclear reprogramming substance
CA2789749C (en) * 2010-02-16 2018-09-04 Kyoto University Method of efficiently establishing induced pluripotent stem cells
WO2012074106A1 (ja) 2010-12-03 2012-06-07 国立大学法人京都大学 多能性幹細胞からの好酸球の製造方法
EP2647699B1 (en) * 2010-12-03 2020-04-01 Kyoto University Efficient method for establishing induced pluripotent stem cells
EP2695894B1 (en) 2011-04-08 2017-11-01 Osaka University Modified laminin and use thereof
WO2012141181A1 (ja) * 2011-04-11 2012-10-18 国立大学法人京都大学 核初期化物質
KR101334404B1 (ko) * 2011-04-28 2013-12-12 포항공과대학교 산학협력단 배아줄기세포 유래의 인공 마이크로베시클을 이용한 역분화 유도만능줄기세포의 제조방법
KR101791926B1 (ko) 2011-07-25 2017-11-20 고쿠리츠 다이가쿠 호진 교토 다이가쿠 유도 만능 줄기 세포 스크리닝 방법
US20140248698A1 (en) 2011-10-21 2014-09-04 Arkray, Inc. Method for culturing pluripotency-maintained singly dispersed cells by means of laminar flow
EP2784153B1 (en) 2011-11-25 2018-01-03 Kyoto University Method for culturing pluripotent stem cell
EP2852671B1 (en) * 2012-05-21 2018-08-22 The Regents of the University of California Generation of human ips cells by a synthetic self- replicative rna
US20160002599A1 (en) 2013-02-08 2016-01-07 Kyoto University Production methods for megakaryocytes and platelets
US9738861B2 (en) 2013-03-06 2017-08-22 Kyoto University Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells
US10519421B2 (en) 2013-03-21 2019-12-31 Kyoto University Induction of motor neurons from pluripotent stem cells
JP6473686B2 (ja) 2013-03-25 2019-02-20 公益財団法人神戸医療産業都市推進機構 細胞の選別方法
JP6461787B2 (ja) 2013-04-12 2019-01-30 国立大学法人京都大学 肺胞上皮前駆細胞の誘導方法
EP2998391B1 (en) 2013-05-14 2020-02-19 Kyoto University Efficient myocardial cell induction method
KR101870174B1 (ko) 2013-05-31 2018-06-22 아이하트 재팬 가부시키가이샤 하이드로겔을 포함하는 적층화된 세포 시트
MX2015017036A (es) 2013-06-11 2016-07-21 Univ Kyoto Metodo para producir celulas progenitoras renales y farmaco que comprende las mismas.
US10557120B2 (en) 2013-07-26 2020-02-11 Kyoto Prefectural Public University Corporation Osteoblast and method for preparing same
EP3031905A4 (en) 2013-08-07 2017-04-26 Kyoto University Method for producing pancreatic hormone-producing cell
CN121136933A (zh) 2013-09-05 2025-12-16 国立大学法人京都大学 由多能干细胞制造产多巴胺神经前体细胞的方法、产多巴胺神经前体细胞、帕金森病治疗剂和试剂盒
EP3064577B1 (en) 2013-11-01 2020-09-09 Kyoto University Novel chondrocyte induction method
EP3929302A1 (en) 2014-07-14 2021-12-29 Chugai Seiyaku Kabushiki Kaisha Method for identifying epitope on protein
CN107075484B (zh) 2014-07-18 2022-01-28 国立大学法人京都大学 从多能性干细胞诱导细胞免疫治疗用t细胞的方法
US10711249B2 (en) 2014-12-26 2020-07-14 Kyoto University Method for inducing hepatocytes
EP3081638A1 (en) 2015-04-16 2016-10-19 Kyoto University Method for producing pseudo-islets
WO2017059241A1 (en) 2015-10-02 2017-04-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Lentiviral protein delivery system for rna-guided genome editing
AU2017248985B2 (en) 2016-04-15 2023-01-12 Kyoto University Method for inducing antigen specific CD8 positive T cells
CN109072198B (zh) 2016-04-22 2022-08-26 国立大学法人京都大学 产多巴胺神经祖细胞的制备方法
KR102537361B1 (ko) 2016-12-27 2023-05-26 스미또모 가가꾸 가부시키가이샤 인공 다능성 줄기 세포의 평가 방법 및 선발 방법, 그리고 인공 다능성 줄기 세포의 제조 방법
CN110199017A (zh) 2017-01-20 2019-09-03 国立大学法人京都大学 CD8α+β+细胞毒性T细胞的制备方法
EP3575392A4 (en) 2017-01-26 2020-08-26 Osaka University MEDIUM INTENDED TO INDUCE DIFFERENTIATION OF STEM CELLS INTO MESODERMAL CELLS AND PROCESS INTENDED TO PRODUCE MESODERMAL CELLS
JP7171055B2 (ja) 2017-03-14 2022-11-15 国立大学法人京都大学 多能性幹細胞からヘルパーｔ細胞を製造する方法
JP7161775B2 (ja) 2017-05-25 2022-10-27 国立大学法人京都大学 中間中胚葉細胞から腎前駆細胞への分化誘導方法、および多能性幹細胞から腎前駆細胞への分化誘導方法
CN111164209A (zh) 2017-06-19 2020-05-15 公益财团法人神户医疗产业都市推进机构 多能干细胞的分化能力的预测方法和用于该预测方法的试剂
JP7140400B2 (ja) 2017-10-17 2022-09-21 国立大学法人京都大学 多能性幹細胞から人工神経筋接合部を得る方法
WO2020013315A1 (ja) 2018-07-13 2020-01-16 国立大学法人京都大学 γδＴ細胞の製造方法
JP7285015B2 (ja) 2018-07-19 2023-06-01 国立大学法人京都大学 多能性幹細胞由来の板状軟骨およびその製造方法
EP3828262A4 (en) 2018-07-23 2022-03-30 Kyoto University Novel renal progenitor cell marker and method for concentrating renal progenitor cells using same
WO2020116606A1 (ja) 2018-12-06 2020-06-11 キリンホールディングス株式会社 Ｔ細胞又はｎｋ細胞の製造方法、ｔ細胞又はｎｋ細胞の培養用培地、ｔ細胞又はｎｋ細胞の培養方法、未分化ｔ細胞の未分化状態を維持する方法及びｔ細胞又はｎｋ細胞の増殖促進剤
US12435311B2 (en) 2018-12-21 2025-10-07 The University Of Osaka Lubricin-localized cartilage-like tissue, method for producing same and composition comprising same for treating articular cartilage damage
CN113226475A (zh) 2018-12-26 2021-08-06 麒麟控股株式会社 改造tcr及其制造方法
JP7692610B2 (ja) 2019-02-26 2025-06-16 国立大学法人東北大学 ｉＰＳ細胞を用いた骨芽細胞塊の作製法
KR20220008894A (ko) 2019-05-15 2022-01-21 아지노모토 가부시키가이샤 신경제세포 또는 각막 상피 세포의 순화 방법
JPWO2020235319A1 (OSRAM) 2019-05-20 2020-11-26
JP7058431B2 (ja) 2019-12-12 2022-04-22 国立大学法人千葉大学 巨核球および血小板を含む凍結乾燥製剤
BR112022017216A2 (pt) 2020-02-28 2022-10-11 Takeda Pharmaceuticals Co Método para produzir células exterminadoras naturais a partir de células-tronco pluripotentes
WO2021256522A1 (ja) 2020-06-17 2021-12-23 国立大学法人京都大学 キメラ抗原受容体発現免疫担当細胞
US20230256024A1 (en) 2020-07-13 2023-08-17 Kyoto University Skeletal muscle precursor cells and method for purifying same, composition for treating myogenic diseases, and method for producing cell group containing skeletal muscle precursor cells
EP4180514A4 (en) 2020-07-20 2024-09-04 Aichi Medical University COMPOSITION FOR UNDIFFERENTIATED MAINTENANCE CULTURE OF PLURIPOTENT CELLS, MEDIUM FOR UNDIFFERENTIATED MAINTENANCE CULTURE OF PLURIPOTENT CELLS, METHOD FOR MAINTENANCE CULTURE IN UNDIFFERENTIATED STATE OF PLURIPOTENT CELLS, AND METHOD FOR PRODUCING PLURIPOTENT CELLS
JPWO2022039279A1 (OSRAM) 2020-08-18 2022-02-24
EP4310176A4 (en) 2021-03-17 2025-03-26 Astellas Pharma Inc. Pericyte with basic fibroblast growth factor (BFGF) gene inserted into it
CA3214490A1 (en) 2021-03-25 2022-09-29 Bluerock Therapeutics Lp Methods for obtaining induced pluripotent stem cells
US20240216440A1 (en) 2021-04-30 2024-07-04 Riken String-like aggregates of retinal pigment epithelial cells, device and production method for producing same, and therapeutic agent comprising said string-like aggregates
WO2022255489A1 (ja) 2021-06-04 2022-12-08 キリンホールディングス株式会社 細胞組成物、細胞組成物の製造方法及び細胞組成物を含む医薬組成物
WO2022259721A1 (ja) 2021-06-10 2022-12-15 味の素株式会社 間葉系幹細胞の製造方法
CA3222770A1 (en) 2021-06-15 2022-12-22 Takeda Pharmaceutical Company Limited Method for producing natural killer cells from pluripotent stem cells
WO2023286834A1 (ja) 2021-07-15 2023-01-19 アステラス製薬株式会社 血管内皮増殖因子（ｖｅｇｆ）高発現ペリサイト様細胞
WO2023286832A1 (ja) 2021-07-15 2023-01-19 アステラス製薬株式会社 血管内皮増殖因子（ｖｅｇｆ）高発現ペリサイト様細胞の製造方法
EP4386083A4 (en) 2021-08-11 2025-11-12 Univ Kyoto METHOD FOR PRODUCING RENAL INTERSTITIAL PROGENITOR CELLS, ERYTHROPOIETIN-PRODUCING CELLS AND METHOD FOR PRODUCING RENIIN-PRODUCING CELLS
CN118265792A (zh) 2021-11-11 2024-06-28 希里欧斯株式会社 基因修饰多能干细胞、来自其的免疫活性细胞、它们的制造方法和它们的用途
JPWO2023153464A1 (OSRAM) 2022-02-09 2023-08-17
WO2023210713A1 (ja) 2022-04-27 2023-11-02 国立大学法人京都大学 心外膜細胞再生促進剤および心外膜細胞の再生促進方法
US20250376727A1 (en) 2022-06-10 2025-12-11 Kyoto University Detection method and detection reagent for undifferentiated pluripotent stem cells
WO2024248017A1 (ja) 2023-05-29 2024-12-05 国立研究開発法人理化学研究所 心筋細胞を成熟させる方法及び成熟した心筋細胞の製造方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
EP1186667B1 (en) 1999-05-18 2007-07-18 Dnavec Research Inc. Envelope gene-deficient virus vector of paramyxoviridae
US8048999B2 (en) * 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
EP2137296A2 (en) * 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
CN101932705A (zh) * 2007-04-07 2010-12-29 怀特黑德生物医学研究所 体细胞重编程
JP2010528622A (ja) * 2007-05-30 2010-08-26 ザ ジェネラル ホスピタル コーポレイション 体細胞から多能性細胞を生成する方法
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
CA2660123C (en) 2007-10-31 2017-05-09 Kyoto University Nuclear reprogramming method
JPWO2010027062A1 (ja) 2008-09-04 2012-02-02 独立行政法人理化学研究所 Ｂ細胞由来ｉＰＳ細胞およびその用途
WO2010027094A1 (ja) 2008-09-08 2010-03-11 独立行政法人理化学研究所 NKT細胞由来iPS細胞およびそれ由来のNKT細胞
WO2010098419A1 (en) 2009-02-27 2010-09-02 Kyoto University Novel nuclear reprogramming substance
CN101613717B (zh) 2009-04-17 2012-01-11 中国科学院广州生物医药与健康研究院 用猪成纤维细胞生成诱导的多能性干细胞的方法
CA2770412C (en) 2009-08-07 2018-09-11 Kyoto University Method of efficiently establishing induced pluripotent stem cells
CA2789749C (en) 2010-02-16 2018-09-04 Kyoto University Method of efficiently establishing induced pluripotent stem cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Cloning and characterization of a novel bicoid-related homebox transcription fator gene,RIEG,involved in Rieger syndrome;Elena et al;《Nature genetics》;19961231;第14卷;附图2 *
Identification of Glis1, a Novel Gli-related, Kruppel-like Zinc Finger Protein Containing Transactivation and Repressor Functions;Yong et al;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;20020830;第227卷(第34期);摘要，附图1 *
NM_024335.1;Ogura et al;《Genbank》;20040209 *
Novel Paralogy Relations Among Human Chromosomes Support a Link between the Phylogeny of doublesex-Related Genes and the Evolution of Sex Determination;Chris Ottolenghi et al;《GENOMICS》;20020331;第79卷(第3期);摘要，表1 *
Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb;Bo Feng et al;《nature cell biology》;20091111;第11卷(第2期);197-203 *

Also Published As

Publication number Publication date
CA2753845A1 (en) 2010-09-02
IL214833A0 (en) 2011-11-30
CN102388136A (zh) 2012-03-21
IL214833A (en) 2016-07-31
JP2012518988A (ja) 2012-08-23
SG173876A1 (en) 2011-09-29
KR20120003867A (ko) 2012-01-11
EP2607476A1 (en) 2013-06-26
CA2753845C (en) 2019-10-29
WO2010098419A1 (en) 2010-09-02
EP2607476B1 (en) 2015-04-08
EP2401372A1 (en) 2012-01-04
US8951801B2 (en) 2015-02-10
US20120052583A1 (en) 2012-03-01
KR101764100B1 (ko) 2017-08-02
EP2401372A4 (en) 2012-09-19
JP5553289B2 (ja) 2014-07-16

Similar Documents

Publication Publication Date Title
CN102388136B (zh) 新颖的核重编程序物质
JP5765746B2 (ja) 効率的な人工多能性幹細胞の樹立方法
EP2526189B1 (en) Method for improving induced pluripotent stem cell generation efficiency
JP5888753B2 (ja) 効率的な人工多能性幹細胞の樹立方法
CN101848994A (zh) 有效建立诱导的多能干细胞的方法
EP2480657B1 (en) Method of efficiently establishing induced pluripotent stem cells
WO2011055851A1 (en) Method of efficiently establishing induced pluripotent stem cells
WO2012141181A1 (ja) 核初期化物質
WO2013031826A1 (ja) 核初期化物質

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant