CN102366478A - Composition for whitening skin and resisting senescence, preparation method and application thereof - Google Patents
Composition for whitening skin and resisting senescence, preparation method and application thereof Download PDFInfo
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- CN102366478A CN102366478A CN2011103591419A CN201110359141A CN102366478A CN 102366478 A CN102366478 A CN 102366478A CN 2011103591419 A CN2011103591419 A CN 2011103591419A CN 201110359141 A CN201110359141 A CN 201110359141A CN 102366478 A CN102366478 A CN 102366478A
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Abstract
The invention discloses a composition for whitening skin and resisting senescence, which comprises the components in parts by weight: 20 to 28 parts of peony, 32 to 40 parts of savia miltiorrhiza leaves, 19 to 27 parts of radix astragali and 13 to 21 parts of wolfberry fruit, preferred peony is obtained by eliminating benzoic acid contained in the peony. The invention also discloses a preparation method and application of the composition. According to the invention, four traditional Chinese medicines are adopted as constituents; the composition has the characteristics of simple formation, easiness in obtaining materials and low cost; and moreover, a synergic effect of delaying senescence and whitening skin is obtained. The composition provided by the invention is safe and controllable and does not have harm to health; and the preparation method is simple, convenient for commercial production and easy for popularization.
Description
Technical field
Invention relates to a kind of compositions, health food or the medicine that it is processed, its preparation method and application in the skin whitening slow down aging thereof of the skin whitening slow down aging that is formed by Chinese medicine composition.
Background technology
The personage of the world of medicine is total to knowledge, skin whitening, go the speckle skin care, remain youthful forever, wonderful dream that slow down aging is people, people are always thinking that various ways solve this problem.With advancing age, the ability of the natural free radical resisting of human body, that is the antioxidative ability constantly descends, and people's cell can be old and feeble, and the people also can produce problems such as pachylosis, pigmentation in appearance, and human tissue organ is aging rapidly also.Along with the raising of Chinese economic development and people's living standard, people have had higher requirement to quality of life, skin whitening, go the speckle skin care, remain youthful forever, this worldwide medical science problem of slow down aging also receives publicity in China day by day.
And a lot of Chinese medicines have the advantage of integration of edible and medicinal herbs, are widely used in slow down aging and skin whitening etc.Such as, Radix Paeoniae (
Paeonia lactiflora) peoniflorin, Hydroxy peoniflorin etc. in spending all have antioxidation, can remove free radical, suppress lipid peroxide and form, they all have speckle skin care, antidotal effect; As Radix Salviae Miltiorrhizae (
Salvia miltiorrhizaBge.) phenolic acids, chlorophyll etc. in the leaf has antioxidation, removes free machine, antidotal effect; The Radix Astragali (
Radix Astragali) in the flavones ingredient calycosin, the 3-hydroxyl-9 that contain; 10-dimethoxy Lignum pterocarpi indici alkane and Saponin class composition astragaloside I, V, III etc. all have stronger antioxidation; Can reduce lipid peroxide and liver lipofuscin content in the serum; Radix Astragali total flavones also has the active effect of the SOD of rise, can reduce MDA to biomembranous infringement, and they all can play speckle skin care, antidotal effect; Fructus Lycii (
Lycium barbarum L.) in a lot of polyphenoils of containing; Like rutin of vitamin E, vitamin C, carotene, betanin, flavonoid etc.; They all can suppress the generation of lipid peroxide well, have very strong anti-oxidation function, can play speckle skin care, function in delaying senility.
At present; Report about the compositions of slow down aging skin whitening is also arranged a lot; Disclose in these reports use above-mentioned one or more Chinese medicines preparation be used for defying age and the compositions of whitening etc.; Like publication number is the one Chinese patent application of 101797208A, and and for example publication number is the one Chinese patent application of 101744756A.
These compositionss of reporting at present; Often having a common feature also is its disadvantage; Being that constituent is complicated, is that the constituent of the one Chinese patent application of 101744756A reaches 20 kinds more than like publication number, and other prior composition also are more than at least seven kinds.Particularly, at first, the complicated component of Chinese medicine; A lot of important compositions do not understand fully that as yet too much Chinese medicine mixes, and each other complex reaction can take place; Increased the uncertain risk of Chinese medicine; Especially for being used for medicine or the health product that slow down aging and skin are whitened and used, often need take for a long time, thereby increase side reaction even the carcinogenic risk of pathogenic teratogenesis; Secondly, of a great variety, also bring difficulty for the collection of production cost, raw material, the manufacturer that especially owes to enrich for natural resources of Chinese medicinal materials has increased its production cost, directly influences the business economic benefit.
In addition, though there are some medicines to use flower of Paeonia lactiflora Pal1. at present, it is reported and according to the application inventor's checking; Generally contain the benzoic acid more than 1.5% in the flower of Paeonia lactiflora Pal1.; It has very strong Liver and kidney toxicity and carcinogenic danger, does not remove these benzoic acid, can cause very big harm to human body; Especially the slow down aging of taking for a long time for needs and the medicine of skin whitening more can strengthen the accumulation of harmful substance and cause the risk of Health cost.
For Radix Salviae Miltiorrhizae, on the one hand, there is report to adopt Radix Salviae Miltiorrhizae and other compatibilities as whitening anti-wrinkling; Such as publication number is the Chinese patent of CN101524320, and the component that this patent adopted is less relatively, is five kinds of compositions; But well known to a person skilled in the art: Radix Salviae Miltiorrhizae contains a large amount of tanshinone components, and this constituents has very strong cardiotonic; If take for a long time, can cause damage to cardiovascular, and as the medicine of whitening anti-wrinkling; Must take for a long time again, so will inevitably threaten health.On the other hand, at present Radix Salviae Miltiorrhizae has realized that the GAP kind plants, especially the Zhongjiang County, Sichuan Province at applicant place; As well-known Radix Salviae Miltiorrhizae GAP planting base, in the growth course of Radix Salviae Miltiorrhizae, can produce a large amount of Radix Salviae Miltiorrhizae leaves; Radix Salviae Miltiorrhizae leaf such as noted earlier; Comparatively similar with the chemical constituent of Radix Salviae Miltiorrhizae, different places is that mainly wherein tanshinone component content is few relatively a lot, and liposoluble ingredient content is more; Especially the Radix Salviae Miltiorrhizae GAP planting base of Zhongjiang County the Da Ye Radix Salviae Miltiorrhizaes of a large amount of plantations, the characteristics of this chemical constituent are particularly evident.At present for the Radix Salviae Miltiorrhizae leaf, generally be that it is discarded, also there is report to be made into Folium Camelliae sinensis, be the Chinese patent of CN101485374A such as publication number, this patent is only processed Folium Camelliae sinensis with the Radix Salviae Miltiorrhizae leaf, and the effect of its slow down aging of whitening is obviously not enough.
Summary of the invention
One of goal of the invention of the present invention is the problem to above-mentioned existence, and the compositions that a kind of constituent is simple, with low cost and the skin whitening defying age is effective is provided.
The technical scheme that the present invention adopts is such: the antidotal compositions of a kind of skin whitening, its component is by weight: 20～28 parts of flower of Paeonia lactiflora Pal1., 32～40 parts on Radix Salviae Miltiorrhizae leaf, 19～27 parts of the Radixs Astragali, 13～21 parts of Fructus Lycii.
The inventor is through a large amount of experiments; Flower of Paeonia lactiflora Pal1., Radix Salviae Miltiorrhizae leaf, the Radix Astragali, these four kinds of the most common Chinese medicines of Fructus Lycii have been screened; Form new compositions, and confirmed the proportion that it is suitable, and through experimental verification the effectiveness of its slow down aging skin whitening; The effect that proves its slow down aging skin whitening simultaneously be better than independent employing above-mentioned any one, the effect of two kinds, three kinds Chinese medicines, i.e. the present invention is the synergistic function that has the slow down aging skin whitening between the compositions.
As preferably: its component is by weight: 24 parts of flower of Paeonia lactiflora Pal1., 36 parts on Radix Salviae Miltiorrhizae leaf, 23 parts of the Radixs Astragali, 17 parts of Fructus Lycii.Through a large amount of experiments, obtained the ratio of the best slow down aging skin whitening effect of four kinds of components.
Two of goal of the invention of the present invention is to provide a kind of compositions of safer slow down aging skin whitening.
The technical scheme that adopts is: said flower of Paeonia lactiflora Pal1. removes the flower of Paeonia lactiflora Pal1. that wherein contained benzoic acid is handled for process.
As preferably: to be controlled at mass percent be below 0.075% to benzoic acid content in the said flower of Paeonia lactiflora Pal1..
Remove that benzoic method is in the flower of Paeonia lactiflora Pal1.:
Select the flower of Paeonia lactiflora Pal1. when half-open or in full bloom, clean with clear water, drying in the shade then comes off naturally or one touches the degree that promptly comes off to petal, removes impurity such as floral disc, pistil, sepal; Petal is placed green-keeping machine, keep the unimpeded of green-keeping machine upper air, lasting 50～70 second moment completes and removes benzoic acid under 285 ℃～315 ℃ temperature, takes out; Put coldly, then petal is put in the baking oven, keep the baking oven upper air unimpeded, under 200 ℃～220 ℃ temperature, toasted 2～3 minutes; Further to remove benzoic acid, take out, put cold; Put in the baking oven in 102 ℃～104 ℃ temperature dry by the fire to moisture content be 8%～12%, take out, put cold getting final product.
Three of the object of the invention is; A kind of method for preparing of above-mentioned composition is provided; The technical scheme that adopts is: prepare flower of Paeonia lactiflora Pal1., Radix Salviae Miltiorrhizae leaf, the Radix Astragali and Fructus Lycii respectively, mix getting final product then according to described part by weight, wherein processing of flower of Paeonia lactiflora Pal1. and preparation are as stated.
The preparation of Radix Salviae Miltiorrhizae leaf:
Select 80～180 days Radix Salviae Miltiorrhizae blade of growth; Clean with clear water, put evenly at the stand, the soft and broken degree of blade when holding blade of drying in the shade; Blade after in green-keeping machine, will drying continues 50～70 second moment and completes under 350 ℃～420 ℃ high temperature; Blade after completing is placed on the blade that eases back in the container rubs and shatter-proof degree, the blade that eases back is kneaded the compact bending of nature with kneading machine with blade till, put in the baking oven and after toasting 2～3 minutes under 130 ℃～140 ℃ the temperature, pour out; Naturally deposited 20～40 minutes; After toasting 2～4 minutes under 200 ℃～220 ℃ the temperature, pour out then, put and be chilled to room temperature, putting in the baking oven and being baked to water content in 50 ℃～60 ℃ temperature is to get final product in 10%～13% o'clock.
Radix Astragali preparation of sections:
Get the Radix Astragali, clean fast with clear water, place, let moisture content immerse Radix Astragali inside so that the Radix Astragali is moistening, be cut into the thin slice that thickness is 2～3mm, putting in the baking oven and being baked to water content in 102 ℃～105 ℃ temperature is 8%～12%, and sealing is preserved;
The preparation of Fructus Lycii:
Get Fructus Lycii, clean fast with clear water, place, dry the moisture content of surface adhesion, putting in the baking oven and being baked to water content in 102 ℃～105 ℃ temperature is 10%～14%, and sealing is preserved.
Four of the object of the invention is; The application of above-mentioned composition is provided; The technical scheme that adopts is: process tea through flower of Paeonia lactiflora Pal1., Radix Salviae Miltiorrhizae leaf, the Radix Astragali and the Fructus Lycii handled through the method for preparing tea with above; Perhaps the Radix Salviae Miltiorrhizae leaf perhaps all uses postcritical method to extract active ingredient respectively with all the other flower of Paeonia lactiflora Pal1. of diafiltration, the Radix Astragali and several kinds of methods with decocting of Fructus Lycii, adds suitable adjuvant then and processes suitable dosage form such as granule, tablet, syrup, oral liquid.
In sum, owing to adopted technique scheme, the invention has the beneficial effects as follows:
At first, adopted four kinds of Chinese medicines as constituent, formed simply, material is easy to obtain, and is with low cost, and obtained the cooperative synergism effect that the slow down aging skin is whitened;
Secondly, compositions safety of the present invention is controlled, is safe from harm for health.Particularly; On the one hand because material is a common Chinese medicine; Its contained chemical constituent is clear controlled, on the other hand, adopts simple method to remove in known harmful components benzoic acid; Further guaranteed to take for a long time the safety of this compositions; Specifically be the benzoic acid that utilizes in the flower of Paeonia lactiflora Pal1. to be contained be the characteristics that exist with the free state form and when temperature is 100 ℃ the characteristics of distillation rapidly, adopts method simple and convenient, with low cost that it is removed, and has kept peoniflorin wherein, Hydroxy peoniflorin etc. well and have antioxidation, can remove free radical, suppresses the composition of lipid peroxide formation; Through processed of the present invention, these useful compositions are dissolved out more easily; Again on the one hand,, both reduced even eliminated the health risk that the heart tonifying composition of Radix Salviae Miltiorrhizae brings, also previously discarded Radix Salviae Miltiorrhizae leaf had effectively been utilized, prevented the wasting of resources, produced bigger economic benefit and social benefit with the alternative Radix Salviae Miltiorrhizae of Radix Salviae Miltiorrhizae leaf;
At last, preparation method of composition of the present invention is simple, is convenient to suitability for industrialized production, and with low cost, is easy to promote.
The specific embodiment
Do detailed explanation in the face of the present invention down.
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further elaborated below in conjunction with embodiment.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in qualification the present invention.
Embodiment 1: prepare flower of Paeonia lactiflora Pal1., Radix Salviae Miltiorrhizae leaf, the Radix Astragali and Fructus Lycii respectively
The preparation of flower of Paeonia lactiflora Pal1.:
Select flower of Paeonia lactiflora Pal1. in full bloom, clean with clear water, put evenly at the stand; Drying in the shade comes off naturally or one touches and promptly to come off to petal, removes impurity such as floral disc, pistil, sepal, and petal is put in the green-keeping machine; Keep the green-keeping machine upper air unimpeded, lasting 60 second moment completes and removes benzoic acid under 300 ℃ temperature, the petal after completing placed the petal that eases back naturally rub and shatter-proof degree; With the petal that eases back with kneading machine knead nature compact curl till, the petal after kneading is put in the baking oven, keep the baking oven upper air unimpeded; Under 220 ℃ temperature the baking 2 minutes so that the petal molding with further remove benzoic acid, the taking-up; Put coldly, putting in the baking oven and being baked to moisture content in 105 ℃ temperature is to get final product in 10% o'clock.
The preparation of Radix Salviae Miltiorrhizae leaf:
Select 120 days Radix Salviae Miltiorrhizae blade of growth, clean with clear water, put evenly at the stand; Soft and the broken degree of blade when holding blade of drying in the shade, the blade after in green-keeping machine, will drying lasting 60 second moment under 385 ℃ high temperature completes, and the blade after completing is placed on the blade that eases back in the container rub and shatter-proof degree; Till the blade that eases back kneaded the compact bending of nature with kneading machine with blade; Put in the baking oven and pour out after 2 minutes, deposited naturally 30 minutes, pour out after 3 minutes in baking under 210 ℃ the temperature then in baking under 135 ℃ the temperature; Put and be chilled to room temperature, putting in the baking oven and being baked to water content in 55 ℃ temperature is to get final product in 12% o'clock.
Radix Astragali preparation of sections:
Get the Radix Astragali, clean fast with clear water, place, let moisture content immerse Radix Astragali inside so that the Radix Astragali is moistening, be cut into the thin slice that thickness is 3mm, putting in the baking oven and being baked to water content in 103 ℃ temperature is 10%, and sealing is preserved.
The preparation of Fructus Lycii:
Get Fructus Lycii, clean fast with clear water, place, dry the moisture content of surface adhesion, putting in the baking oven and being baked to water content in 103 ℃ temperature is 12%, and sealing is preserved.
With the above flower of Paeonia lactiflora Pal1. for preparing, Radix Salviae Miltiorrhizae leaf, the Radix Astragali and Fructus Lycii according to dry product weight meter, 20 parts of flower of Paeonia lactiflora Pal1., 40 parts on Radix Salviae Miltiorrhizae leaf, 19 parts of the Radixs Astragali, the ratio that Fructus Lycii is 21 parts, mix homogeneously, be distributed into every bag 10 the gram get final product.
Embodiment 2:
A kind of compositions of slow down aging skin whitening, its component is by weight: 20 parts of flower of Paeonia lactiflora Pal1., 40 parts on Radix Salviae Miltiorrhizae leaf, 19 parts of the Radixs Astragali, 21 parts of Fructus Lycii.The method for preparing of present embodiment and embodiment 1 are similar, and difference only is not remove benzoic acid.
Embodiment 3: the preparation of complete petal and blade tea
With the component uniform mixing of embodiment 2, be distributed into every bag of 10g and get final product.
Embodiment 4:
A kind of compositions of slow down aging skin whitening, its component is by weight: 28 parts of flower of Paeonia lactiflora Pal1., 32 parts on Radix Salviae Miltiorrhizae leaf, 27 parts of the Radixs Astragali, 13 parts of Fructus Lycii.The method for preparing of present embodiment and embodiment 1 are similar, and difference only is not remove benzoic acid.
Embodiment 5: the flower of Paeonia lactiflora Pal1. and the salvia root leaf tea that are ground into coarse powder
The component pulverize separately of embodiment 1 is become coarse powder, and mix homogeneously is distributed into every bag of 10g and gets final product then.
Embodiment 6:
A kind of compositions of slow down aging skin whitening, its component is by weight: 24 parts of flower of Paeonia lactiflora Pal1., 36 parts on Radix Salviae Miltiorrhizae leaf, 23 parts of the Radixs Astragali, 17 parts of Fructus Lycii.The method for preparing of present embodiment and embodiment 1 are similar, and difference only is not remove benzoic acid.
Embodiment 7:
A kind of compositions of slow down aging skin whitening, its component is by weight: 24 parts of flower of Paeonia lactiflora Pal1., 36 parts on Radix Salviae Miltiorrhizae leaf, 23 parts of the Radixs Astragali, 17 parts of Fructus Lycii.The method of the method for preparing of present embodiment and embodiment 1 is in full accord.
Embodiment 8: the granule that a kind of compositions of slow down aging skin whitening is processed
The preparation of flower of Paeonia lactiflora Pal1. extractum:
Select half-open flower of Paeonia lactiflora Pal1., clean with clear water, put evenly at the stand, and drying in the shade comes off to petal naturally; Remove impurity such as floral disc, pistil, sepal, petal is put in the green-keeping machine, keep the green-keeping machine upper air unimpeded, lasting 70 second moment completes and removes benzoic acid under 280 ℃ high temperature; Take out, put coldly, then petal is put in the baking oven, keep the baking oven upper air unimpeded; Baking is 2 minutes under 180 ℃ temperature, further to remove benzoic acid, pours out then; Put and coldly put in the baking oven that to be baked to moisture content in 106 ℃ temperature be 12%, take out, put cold.
Flower of Paeonia lactiflora Pal1. after getting above oven dry and removing benzoic acid, the decocting that adds 8 times of dry flower weight boiled 1 hour, filtered; Collect filtrate for later use, the decocting that flower of Paeonia lactiflora Pal1. adds 6 times of dry flower weight again boiled 0.5 hour, filtered; Collect filtrating,, leave standstill with the filtrating merging of front; Get supernatant, being concentrated into relative density is 1.18, promptly gets flower of Paeonia lactiflora Pal1. extractum.
The preparation of Radix Salviae Miltiorrhizae leaf extractum:
Get Da Ye Radix Salviae Miltiorrhizae leaf, be ground into coarse powder, put in the diafiltration jar; Compress, 30～70% the ethanol of getting 5 to 10 times of amounts of Radix Salviae Miltiorrhizae leaf weight adds in the diafiltration jar, just in time exceed 1～2 centimetre of Radix Salviae Miltiorrhizae leaf coarse powder to the ethanol liquid level till; Soak at room temperature 50～100 hours is emitted percolate with the speed of per minute 1～3ml by the tank bottoms end of diafiltration, collects subsequent use; To remain ethanol simultaneously adds from the top of diafiltration jar to emit the same speed of speed with the bottom; Till adding, continue to collect percolate, treat that percolate in the diafiltration jar drains till.The percolate of above collection is filtered, filtrate recycling ethanol, being concentrated into relative density is 1.18～1.22, promptly gets Radix Salviae Miltiorrhizae leaf extractum.
The preparation of Radix Astragali extractum:
Get the Radix Astragali, get the Radix Astragali, clean fast, place, let moisture content immerse Radix Astragali inside so that the Radix Astragali is moistening with clear water; Be cut into the thin slice that thickness is 2～3mm, the decocting that adds 10 times of astragalus weights boiled 1.5 hours, filtered, and collected filtrate for later use; The decocting that the Radix Astragali adds 7 times of weight again boiled 1 hour, filtered, and collected filtrating, with the filtrating merging of front; Leave standstill, get supernatant, being concentrated into relative density is 1.23, promptly gets Radix Astragali extractum.
The preparation of Fructus Lycii extractum:
Get Fructus Lycii, clean fast with clear water, the decocting that adds 12 times of Fructus Lycii weight boiled 1.5 hours, filtered; Collect filtrate for later use, the decocting that Fructus Lycii adds 7 times of weight again boiled 1 hour, filtered; Collect filtrating,, leave standstill with the filtrating merging of front; Get supernatant, being concentrated into relative density is 1.22, promptly gets Fructus Lycii extractum.
Get 1 part of flower of Paeonia lactiflora Pal1. extractum, 1 part of Radix Salviae Miltiorrhizae leaf extractum, 1 part of Radix Astragali extractum, 1 part of Fructus Lycii extractum adds 10 parts of cane sugar powders, processes granule, and drying is distributed into 10 gram/bags, and the lucifuge sealing is preserved and is got final product.
Embodiment 9: the tablet that a kind of compositions of slow down aging skin whitening is processed
Adopt the extractum of four kinds of compositions of embodiment 8 described method preparations, wherein get 1 part of flower of Paeonia lactiflora Pal1. extractum, 1 part of Radix Salviae Miltiorrhizae leaf extractum, 1 part of Radix Astragali extractum, 1 part of Fructus Lycii extractum adds 10 parts of cane sugar powders, processes granule, and drying is distributed into 10 gram/bags, and the lucifuge sealing is preserved and is got final product.
Embodiment 10: the benzoic acid content in the flower of Paeonia lactiflora Pal1. that the method among the embodiment 1 makes is measured:
Measure according to HPLC (" Chinese pharmacopoeia version appendix in 2010 VI D).
Chromatographic condition and system suitability test use octadecylsilane chemically bonded silica to be filler; With methanol-0.02mol/L ammonium acetate (5:95) is mobile phase; The detection wavelength is 230nm.Number of theoretical plate calculates by the benzoic acid peak should be not less than 2000.
The preparation precision of reference substance solution takes by weighing benzoic acid standard substance 20mg, puts in the 50ml measuring bottle, adds the small amount of methanol dissolving and is diluted with water to scale, shakes up, and promptly gets (every 1ml contains benzoic acid 0.4mg).
The preparation of need testing solution
Get flower of Paeonia lactiflora Pal1. and grind to form coarse powder, precision takes by weighing 1.5g, puts in the 50ml volumetric flask, adds the NaOH solution 0.5ml of 1.0mol/L, adds water 10ml, in ultrasonic cleaner, adds water to scale behind the sonic oscillation 10min.
Accurate respectively reference substance solution and each the 10 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, and promptly get.
Use above detection method, respectively to the flower of Paeonia lactiflora Pal1. after handling with method of the present invention and untreated flower of Paeonia lactiflora Pal1. each 3 samples, its benzoic content such as following table 1 as a result:
Table 1: benzoic acid testing result
Can find out that from above experimental data after processing method of the present invention was handled, the benzoic content in the flower of Paeonia lactiflora Pal1. reduced greatly, is removed fully basically, proves that the present invention is feasible.
Embodiment 11: measure Main Ingredients and Appearance peoniflorin, Hydroxy peoniflorin in the flower of Paeonia lactiflora Pal1.
Measure by HPLC (" Chinese pharmacopoeia version appendix in 2010 VI D).
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; With acetonitrile-0.1% phosphoric acid solution (14:86) is mobile phase; The detection wavelength is 230nm.Number of theoretical plate calculates by the peoniflorin peak should be not less than 2000.
The preparation precision of reference substance solution takes by weighing peoniflorin and the Hydroxy peoniflorin reference substance is an amount of, adds methanol and processes the solution that every 1ml contains peoniflorin 60 μ g and Hydroxy peoniflorin 30 μ g, promptly gets.
The preparation of need testing solution is got flower of Paeonia lactiflora Pal1. and is ground to form coarse powder, gets 0.2g, and accurate the title decides, and puts in the 100ml measuring bottle, adds Diluted Alcohol 50ml; Supersound process (power 240W, frequency 45kHz) 30 minutes is taken out, and puts coldly, adds methanol to scale; Shake up, filter, get subsequent filtrate, promptly get.
Accurate respectively reference substance solution and each the 10 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, and promptly get.
Table 2: peoniflorin, Hydroxy peoniflorin testing result
Can find out by above experimental data, after processing method of the present invention is handled, in the flower of Paeonia lactiflora Pal1. mainly contain the effective constituent peoniflorin, Hydroxy peoniflorin is not affected, and proves that method of the present invention is feasible.
Embodiment 12
The oxidation and removing free radicals activity experiment of compositions of the present invention:
One, the active experiment of restraint of tyrosinase
1, instrument and reagent
FA2004 electronic analytical balance (going up the flat Instr Ltd. of current chart)
UV762 ultraviolet-uisible spectrophotometer (Shanghai analytical tool factory)
Tryrosinase, Vc ethylether, arbutin are that Chengdu Delai Biology Science Co., Ltd provides.
2, the preparation of sample
Take by weighing flower of Paeonia lactiflora Pal1. 20g, Radix Salviae Miltiorrhizae leaf 40g, Radix Astragali 19g, Fructus Lycii 21g (flower of Paeonia lactiflora Pal1. is handled through the method for embodiment 1) adds water 1000ml; Put in the flask and heat, keep little and boiled 1.5 hours, filter, collect filtrate for later use, add water 1000ml again; Keep little and boiled 1 hour, filter, collect filtrating, with the filtrating merging of front; Leave standstill, get supernatant, be concentrated into 200ml, promptly get.
3, method of testing
Getting four test tubes puts on respectively No. 1, No. 2, No. 3, No. 4; Reagent dosage and concentration with reference to table 3; In vitro add respectively at four and respectively to be listed as listed respective reaction liquid, jolting is evenly mixed, in 37 C preheating 10min; In vitro add tryrosinase solution respectively at each then; In 37 C accurate response 5min, be transferred to rapidly in the cuvette, survey absorbance (A) in the 475nm place;
4, the result calculates and statistics
Be calculated as follows suppression ratio to tryrosinase:
Suppression ratio=[1-(A3-A4)/(A1-A2)] * 100%
In the formula: A1, A2, A3, the A4 absorbance that the reaction back records in the 475nm place in No. 1, No. 2, No. 3, No. 4 four test tubes in the representative table 3 respectively.
Table 3: the composition (unit: ml) of liquid to be measured
Characterize the effect of inhibitor with the inhibitor concentration (IC50) that causes tryrosinase vigor decline 50%.The active testing result of restraint of tyrosinase is seen table 4.
Table 4: each sample suppresses the active testing result of tyrosine
Above result shows that compositions extracting solution of the present invention has stronger tyrosinase activity to suppress ability, and its activity is also suitable with Vc ethylether, arbutin.
Simultaneously, carried out the contrast experiment
The laboratory sample that this contrast experiment adopts is following: (ratio is mass ratio), the quality of each sample of employing are all the same
No. 1: flower of Paeonia lactiflora Pal1.; No. 2: the Radix Salviae Miltiorrhizae leaf; No. 3: the Radix Astragali; No. 4: Fructus Lycii; No. 5: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf (1:1); No. 6: flower of Paeonia lactiflora Pal1.+Radix Astragali (1:1); No. 7: flower of Paeonia lactiflora Pal1.+Fructus Lycii (1:1); No. 8: Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1); No. 9: Radix Salviae Miltiorrhizae leaf+Radix Astragali (1:1); No. 10: Fructus Lycii+Radix Astragali (1:1); No. 11: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf+Radix Astragali (1:1:1); No. 12: flower of Paeonia lactiflora Pal1.+Radix Astragali+Fructus Lycii (1:1:1); No. 13: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1:1); No. 14: the Radix Astragali+Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1:1); No. 15: the compositions of embodiment 2; No. 16: the compositions of embodiment 4; No. 17: the compositions of embodiment 7;
The employing said method experimentizes, experimental result such as table 5:
Table 5: each sample suppresses the active testing result of tyrosine (unit: mg/ml)
Two, the removing of DPPH free radical is tested
1. instrument and reagent
UV762 ultraviolet-uisible spectrophotometer (Shanghai analytical tool factory)
FA2004 electronic analytical balance (going up the flat Instr Ltd. of current chart)
Free radical (DPPH), Vc ethylether, arbutin are that Chengdu Delai Biology Science Co., Ltd provides, and ethanol is that Kingsoft, Chengdu reagent company limited provides.
2. the preparation of sample
Take by weighing flower of Paeonia lactiflora Pal1. 20g, Radix Salviae Miltiorrhizae leaf 40g, Radix Astragali 19g, Fructus Lycii 21g (flower of Paeonia lactiflora Pal1. is handled through the method for embodiment 1) adds water 1000ml; Put in the flask and heat, keep little and boiled 1.5 hours, filter, collect filtrate for later use, add water 1000ml again; Keep little and boiled 1 hour, filter, collect filtrating, with the filtrating merging of front; Leave standstill, get supernatant, be concentrated into 200ml, promptly get.
3. non-oxidizability experiment
3.1 sample is to the removing experiment of DPPH free radical
The preparation of DPPH solution:
Accurately take by weighing DPPH7.88mg, place the 100ml volumetric flask, to scale, promptly getting concentration is the DPPH solution of 2 * 10-4mo1/l with anhydrous alcohol solution, and (0～4 ℃) keeps in Dark Place.
The preparation of test liquid:
Get the extracting solution 40ml of compositions of the present invention, place the 200ml volumetric flask, with ethanol dilution to scale.
Assay method:
Respectively extracting sample solution 5ml and 2 * 10-4mo1/l DPPH solution 5ml uniform mixing place 30min in the dark, with methanol be blank at the 525nm place its absorbance A of mensuration, and with its clearance rate of computes:
Clearance rate (%)=［ 1-(Ai-Aj)/Ac ］ * 100%
In the formula: Ac:5ml ethanol adds the 5mlDPPH solution absorbency;
Ai:5ml liquid to be measured adds the 5mlDPPH solution absorbency;
Aj:5ml liquid to be measured adds the alcoholic acid absorbance of 5ml.
Calculate clearance rate according to top formula.
Characterize the effect of free radical scavenger with the activity concentration (IC50) of removing DPPH free radical 50%.Anti-DPPH free radical activity testing result is seen table 6.
Table 6: the anti-DPPH free radical activity of each sample testing result
The result shows that compositions extracting solution of the present invention has stronger DPPH radical scavenging activity, and its active and Vc ethylether, arbutin are suitable.
Simultaneously, carried out the contrast experiment
The laboratory sample that this contrast experiment adopts is following: (ratio is mass ratio), the quality of each sample of employing are all the same,
No. 1: flower of Paeonia lactiflora Pal1.; No. 2: the Radix Salviae Miltiorrhizae leaf; No. 3: the Radix Astragali; No. 4: Fructus Lycii; No. 5: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf (1:1); No. 6: flower of Paeonia lactiflora Pal1.+Radix Astragali (1:1); No. 7: flower of Paeonia lactiflora Pal1.+Fructus Lycii (1:1); No. 8: Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1); No. 9: Radix Salviae Miltiorrhizae leaf+Radix Astragali (1:1); No. 10: Fructus Lycii+Radix Astragali (1:1); No. 11: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf+Radix Astragali (1:1:1); No. 12: flower of Paeonia lactiflora Pal1.+Radix Astragali+Fructus Lycii (1:1:1); No. 13: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1:1); No. 14: the Radix Astragali+Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1:1); No. 15: the compositions of embodiment 2; No. 16: the compositions of embodiment 4; No. 17: the compositions of embodiment 7;
The employing said method experimentizes, experimental result such as table 7:
Table 7: each sample suppresses the active testing result of tyrosine (unit: μ g/ml)
Three, melanocyte test
1, instrument and reagent
XSP-2C biological microscope Shanghai permanent magnetoelectricity scarabaeidae skill company limited
SK2200H ultrasonic cleaner (Shanghai High Kudos Science Instrument Co., Ltd.)
DHP-9052 constant incubator (Shanghai precision instrument company)
Enzyme-linked immunosorbent assay instrument (Shanghai precision instrument company)
LD4-2A centrifuge (Beijing Medical Centrifugal Machine Factory)
UV762 ultraviolet-uisible spectrophotometer (Shanghai analytical tool factory)
FA2004 electronic analytical balance (going up the flat Instr Ltd. of current chart)
Tryrosinase, Vc ethylether, arbutin are that Chengdu Delai Biology Science Co., Ltd provides.
2, the preparation of sample
Take by weighing flower of Paeonia lactiflora Pal1. 20g, Radix Salviae Miltiorrhizae leaf 40g, Radix Astragali 19g, Fructus Lycii 21g (flower of Paeonia lactiflora Pal1. is handled through the method for embodiment 1) adds water 1000ml; Put in the flask and heat, keep little and boiled 1.5 hours, filter, collect filtrate for later use, add water 1000ml again; Keep little and boiled 1 hour, filter, collect filtrating, with the filtrating merging of front; Leave standstill, get supernatant, be concentrated into 200ml, promptly get.
3, mice B16 melanocyte is cultivated
It is that the RPMI-1640 of 10% calf serum is cultivated the B16 melanocyte under 37 ℃, the condition of 5%CO2 that use contains mass fraction, and the cell inoculation amount is 3 * 106/L, and every 3d passes once generation.
4, the mensuration of cell proliferation rate
Select the B16 melanocyte of exponential phase, use 0.25% trypsinization, the adjustment cell concentration is 2 * 104/ml, is inoculated in 96 well culture plates, and every hole 180uL places cultivation 24h in 37 ℃, the incubator of 5%CO2.It is good that mirror is observed growth conditions down, adds respectively and tried thing 20ul/ hole, continues to cultivate 48h.4h adds the MTT30uL/ hole before finishing, and abandons supernatant behind the 4h, adds the DMSO150uL/ hole again, about vibration 10min, measures the absorbance of 475nm wavelength with enzyme-linked immunosorbent assay instrument.
Test result is the propagation almost all unrestraint effects of three kinds of active matters to melanocyte, and the suppression ratio of on cell proliferation all is lower than 10%, i.e. three kinds of basic no cytotoxicities of active matter.
5, intracellular tyrosine enzyme activity determination
Get the culture bottle that the B16 melanocyte is inoculated in 25 ㎝ 2, inoculum concentration is 2 * 105/bottle, is replaced with behind the cultivation 48h and is added with the culture medium of being tried thing, continues to cultivate 48h, not add active matter as contrast.After 0.25% trypsinization, blow and beat into cell suspension, abandon supernatant behind the low-temperature centrifugation, the harvesting agglomerate with the PBS (phosphate buffer) of pH6.8.The NaTDC that adds 1mL0.5%, ice bath 15min, the cell lysis preparation contains the tryrosinase extracting solution.Get this extracting solution 0.5ml, add 0.3% DOPA solution 0.5ml after the temperature in advance in 37 ℃, 37 ℃ of water-bath 5min measure absorbance with ultraviolet spectrophotometer at the 475nm place.
It is suitable to the suppression ratio of tryrosinase and Vc ethylether, arbutin that test result is a compositions extracting solution of the present invention.
Detect through above external activity, find: the whitening of compositions extracting solution of the present invention, activity of fighting against senium are suitable with Vc ethylether, arbutin.
" double-blind method " measure of merit of the present composition:
According to relevant health food check and the requirement of estimating, we have carried out following test:
1, materials and methods
Given the test agent and method for using:
Experimenter's former life, diet are constant, and matched group is taken placebo, and the test-meal group is taken the tea of processing according to the embodiment of the invention 7 described compositionss (every bag heavy 10g); The experimenter takes 3 bags for each person every day at random; Every bag with 150ml brewed in hot water 3 times, 450ml water altogether, drinking-water waste.The test-meal cycle is 3 months.
Detecting instrument:
Skin microscope (optical instrument factory, Shanghai)
Skin detection analytical system (Guangzhou Pu Tian economy and trade company limited)
Skin elasticity tester (permanent Instr Ltd. is built in Shanghai)
Skinanalysis apparatus (Hong Kong many power company limited)
2, study subject and grouping
Study subject:
The experimenter includes standard in:
Select the age at 28～60 years old, physical condition is good, does not have obvious brain, the heart, liver, lung, kidney, BLOOD DISEASE, does not have the history of taking medicine for a long time, and aspiration is tried to guarantee the crowd that cooperates.Experimenter crowd gets rid of following object: the age below 28 years old or more than 60 years old, gestation or women breast-feeding their children, to the health food allergy sufferers; Intentionally, serious disease persons such as liver, kidney and hemopoietic system; Take the article relevant in a short time, have influence on judgement person the result with being tried function.Participating in the case number before the test-meal is 90 examples, after the test-meal effectively the case number be 63 examples (wherein matched group 30 examples, test-meal 32 examples), losing and visiting the case number is 1 example.
Divide into groups:
Study subject is divided into test-meal group and matched group at random, and two groups all have harmony traditionally at sex, age, living and diet.
3, observation index and analysis
Everyone of test-meal group and matched group measured skin brightness flop, dermal melanin changes of contents with skinanalysis apparatus weekly, with skin elasticity tester test skin elasticity, the depth of testing the wrinkle of skin with skin microscope and skin detection analytical system.
As a result, take the second week beginning from study subject, the skin of test-meal group can detect the brightness increase, melanin descends, and around the, the skin of test-meal group can detect elasticity to be strengthened, and wrinkle shoals, and matched group does not change.Detect weekly later on, all useful improvement of above each item index of the skin of test-meal group, and matched group does not change.Since the 10th week, the variation of above each item index of the skin of test-meal group begins to reduce gradually until not measuring.Evidence compositions of the present invention has skin whitening, the effect of slow down aging.
The invention has the beneficial effects as follows and reach through the inhibitory action that free radical scavenging effect, tyrosinase inhibitory action, melanocyte melanin are produced; Test data and human experimentation checking result show that compositions of the present invention has the effect of defying age skin-whitening.
Claims (7)
1. antidotal compositions of skin whitening, its component is by weight: 20～28 parts of flower of Paeonia lactiflora Pal1., 32～40 parts on Radix Salviae Miltiorrhizae leaf, 19～27 parts of the Radixs Astragali, 13～21 parts of Fructus Lycii.
2. the antidotal compositions of skin whitening according to claim 1, it is characterized in that: its component is by weight: 24 parts of flower of Paeonia lactiflora Pal1., 36 parts on Radix Salviae Miltiorrhizae leaf, 23 parts of the Radixs Astragali, 17 parts of Fructus Lycii.
3. the antidotal compositions of skin whitening according to claim 1 is characterized in that: said flower of Paeonia lactiflora Pal1. removes the flower of Paeonia lactiflora Pal1. that wherein contained benzoic acid is handled for process.
4. the antidotal compositions of skin whitening according to claim 3 is characterized in that in the said flower of Paeonia lactiflora Pal1. that it is below 0.075% that benzoic acid content is controlled at mass percent.
5. any made tea of the antidotal compositions of described skin whitening of claim 1 to 4.
6. the dosage form processed of any antidotal compositions of described skin whitening of claim 1 to 4 and pharmaceutically acceptable excipient.
7. the antidotal preparation of compositions method of any described skin whitening of claim 1 to 4 is characterized in that: prepares flower of Paeonia lactiflora Pal1., Radix Salviae Miltiorrhizae leaf, the Radix Astragali and Fructus Lycii respectively, mixes getting final product then according to described part by weight, wherein,
The preparation of flower of Paeonia lactiflora Pal1.:
Select the flower of Paeonia lactiflora Pal1. when half-open or in full bloom, clean with clear water, drying in the shade then comes off naturally or one touches the degree that promptly comes off to petal, removes impurity such as floral disc, pistil, sepal; Petal is placed green-keeping machine, keep the unimpeded of green-keeping machine upper air, lasting 50～70 second moment completes and removes benzoic acid under 285 ℃～315 ℃ temperature, takes out; Put coldly, then petal is put in the baking oven, keep the baking oven upper air unimpeded, under 200 ℃～220 ℃ temperature, toasted 2～3 minutes; Further to remove benzoic acid, take out, put cold; Put in the baking oven in 102 ℃～104 ℃ temperature dry by the fire to moisture content be 8%～12%, take out, put cold getting final product;
The preparation of Radix Salviae Miltiorrhizae leaf:
Select 80～180 days Radix Salviae Miltiorrhizae blade of growth; Clean with clear water, put evenly at the stand, the soft and broken degree of blade when holding blade of drying in the shade; Blade after in green-keeping machine, will drying continues 50～70 second moment and completes under 350 ℃～420 ℃ high temperature; Blade after completing is placed on the blade that eases back in the container rubs and shatter-proof degree, the blade that eases back is kneaded the compact bending of nature with kneading machine with blade till, put in the baking oven and after toasting 2～3 minutes under 130 ℃～140 ℃ the temperature, pour out; Naturally deposited 20～40 minutes; After toasting 2～4 minutes under 200 ℃～220 ℃ the temperature, pour out then, put and be chilled to room temperature, putting in the baking oven and being baked to water content in 50 ℃～60 ℃ temperature is to get final product in 10%～13% o'clock;
Radix Astragali preparation of sections:
Get the Radix Astragali, clean fast with clear water, place, let moisture content immerse Radix Astragali inside so that the Radix Astragali is moistening, be cut into the thin slice that thickness is 2～3mm, putting in the baking oven and being baked to water content in 102 ℃～105 ℃ temperature is 8%～12%, and sealing is preserved;
The preparation of Fructus Lycii:
Get Fructus Lycii, clean fast with clear water, place, dry the moisture content of surface adhesion, putting in the baking oven and being baked to water content in 102 ℃～105 ℃ temperature is 10%～14%, and sealing is preserved.
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| CN103263536A (en) * | 2013-02-04 | 2013-08-28 | 四川逢春制药有限公司 | Skin-whitening freckle-removing anti-aging composite and preparation method and application thereof |
| CN103316150A (en) * | 2013-06-21 | 2013-09-25 | 四川逢春制药有限公司 | Composition for preventing aging and whitening skin as well as preparation method and application thereof |
| CN103989164A (en) * | 2014-05-09 | 2014-08-20 | 深圳市皇都御颜养生科技有限责任公司 | Preparation method of anti-aging health food |
| CN104840380A (en) * | 2015-01-21 | 2015-08-19 | 青岛科技大学 | Natural substance composition and application of same in whitening cosmetic |
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