CN102366478B - Composition for whitening skin and resisting senescence, preparation method and application thereof - Google Patents
Composition for whitening skin and resisting senescence, preparation method and application thereof Download PDFInfo
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- CN102366478B CN102366478B CN2011103591419A CN201110359141A CN102366478B CN 102366478 B CN102366478 B CN 102366478B CN 2011103591419 A CN2011103591419 A CN 2011103591419A CN 201110359141 A CN201110359141 A CN 201110359141A CN 102366478 B CN102366478 B CN 102366478B
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Abstract
The invention discloses a composition for whitening skin and resisting senescence, which comprises the components in parts by weight: 20 to 28 parts of peony, 32 to 40 parts of savia miltiorrhiza leaves, 19 to 27 parts of radix astragali and 13 to 21 parts of wolfberry fruit, preferred peony is obtained by eliminating benzoic acid contained in the peony. The invention also discloses a preparation method and application of the composition. According to the invention, four traditional Chinese medicines are adopted as constituents; the composition has the characteristics of simple formation, easiness in obtaining materials and low cost; and moreover, a synergic effect of delaying senescence and whitening skin is obtained. The composition provided by the invention is safe and controllable and does not have harm to health; and the preparation method is simple, convenient for commercial production and easy for popularization.
Description
Technical field
Invention relates to a kind of compositions, health food or the medicine that it is made, its preparation method and application in the skin whitening slow down aging thereof of the skin whitening slow down aging that is formed by Chinese medicine composition.
Background technology
The personage of the world of medicine is in common knowledge, skin whitening, go the speckle skin care, remain youthful forever, wonderful dream that slow down aging is people, people are always thinking that various ways solve this problem.With advancing age, the ability of the natural free radical resisting of human body, that is the antioxidative ability constantly descends, and people's cell can be old and feeble, and the people also can produce the problems such as pachylosis, pigmentation in appearance, and human tissue organ is rapidly aging also.Along with the raising of China's expanding economy and people's living standard, people have had higher requirement to quality of life, skin whitening, go the speckle skin care, remain youthful forever, this worldwide medical science problem of slow down aging also receives publicity day by day in China.
And a lot of Chinese medicines have the advantage of integration of edible and medicinal herbs, are widely used in slow down aging and skin whitening etc.Such as, Radix Paeoniae (
Paeonia lactiflora) peoniflorin, Hydroxy peoniflorin etc. in spending all have antioxidation, can remove free radical, suppress lipid peroxide and form, they all have speckle skin care, antidotal effect; As Radix Salviae Miltiorrhizae (
Salvia miltiorrhizaBge.) phenolic acids, chlorophyll etc. in the leaf has antioxidation, removes free machine, antidotal effect; The Radix Astragali (
Radix Astragali) in the flavones ingredient calycosin, the 3-hydroxyl-9 that contain, 10-dimethoxy Lignum pterocarpi indici alkane and Saponin class composition astragaloside I, V, III etc. all have stronger antioxidation, can reduce lipid peroxide and liver lipofuscin content in the serum, Radix Astragali total flavones also has the effect of SOD activity, can reduce lipid peroxide to biomembranous infringement, they all can play speckle skin care, antidotal effect; Fructus Lycii (
Lycium barbarum L.) in a lot of polyphenoils of containing, such as rutin of vitamin E, vitamin C, carotene, betanin, flavonoid etc., they all can suppress the generation of lipid peroxide well, have very strong anti-oxidation function, can play the effect of speckle skin care, slow down aging.
At present, report about the compositions of slow down aging skin whitening is also arranged a lot, the compositions that is used for defying age and whitening etc. of using above-mentioned one or more Chinese drug preparations is disclosed in these reports, be the Chinese patent application of 101797208A such as publication number, and for example publication number is the Chinese patent application of 101744756A.
These compositionss of reporting at present, often having a common feature also is its disadvantage, being that constituent is complicated, is that the constituent of the Chinese patent application of 101744756A reaches 20 kinds more than such as publication number, and other existing compositionss also are more than at least seven kinds.Particularly, at first, the complicated component of Chinese medicine, a lot of important compositions understand that not yet fully too much Chinese medicine mixes, and each other complex reaction can occur, increased the uncertain risk of Chinese medicine, especially for the medicine or the health product that are used for slow down aging and skin whitening use, often need long-term taking, thereby increased side reaction even the carcinogenic risk of pathogenic teratogenesis; Secondly, of a great variety, also bring difficulty for the collection of production cost, raw material, the manufacturer that especially owes to enrich for natural resources of Chinese medicinal materials has increased its production cost, directly affects Business Economic Benefit.
In addition, although being arranged at present, some medicines use flower of Paeonia lactiflora Pal1., but it is reported and according to the present application people's checking, generally contain the benzoic acid more than 1.5% in the flower of Paeonia lactiflora Pal1., it has very strong Liver and kidney toxicity and carcinogenic danger, does not remove these benzoic acid, can cause very large harm to human body, especially for the medicine of the slow down aging that needs long-term taking and skin whitening, more can strengthen the accumulation of harmful substance and cause the risk of Health cost.
For Radix Salviae Miltiorrhizae, on the one hand, there is report to adopt Radix Salviae Miltiorrhizae and other compatibilities as whitening anti-wrinkling, be the Chinese patent of CN101524320 such as publication number, the component that this patent adopts is relatively less, be five kinds of compositions, but, well known to a person skilled in the art: Radix Salviae Miltiorrhizae contains a large amount of tanshinone components, and this constituents has very strong cardiotonic, if long-term taking, can cause damage to cardiovascular, and as the medicine of whitening anti-wrinkling, again must long-term taking, so will inevitably threaten to health.On the other hand, at present Radix Salviae Miltiorrhizae has realized that the GAP kind plants, especially the Zhongjiang County, Sichuan Province at applicant place, as well-known Radix Salviae Miltiorrhizae GAP planting base, in the growth course of Radix Salviae Miltiorrhizae, can produce a large amount of Radix Salviae Miltiorrhizae leaves, the Radix Salviae Miltiorrhizae leaf as previously described, comparatively similar to the chemical constituent of Radix Salviae Miltiorrhizae, different places is that mainly wherein tanshinone component content is relatively few a lot, and liposoluble ingredient content is more, especially the Radix Salviae Miltiorrhizae GAP planting base of Zhongjiang County the Da Ye Radix Salviae Miltiorrhizaes of a large amount of plantations, the characteristics of this chemical constituent are particularly evident.At present for the Radix Salviae Miltiorrhizae leaf, generally be that it is discarded, also there is report to be made into Folium Camelliae sinensis, be the Chinese patent of CN101485374A such as publication number, this patent is only made Folium Camelliae sinensis with the Radix Salviae Miltiorrhizae leaf, and the effect of its whitening slow down aging is obviously not enough.
Summary of the invention
One of goal of the invention of the present invention is the problem for above-mentioned existence, and the compositions that a kind of constituent is simple, with low cost and the skin whitening defying age is effective is provided.
The technical solution used in the present invention is such: the antidotal compositions of a kind of skin whitening, its component is by weight: 20～28 parts of flower of Paeonia lactiflora Pal1., 32～40 parts on Radix Salviae Miltiorrhizae leaf, 19～27 parts of the Radixs Astragali, 13～21 parts of Fructus Lycii.
The inventor is through great many of experiments, flower of Paeonia lactiflora Pal1., Radix Salviae Miltiorrhizae leaf, the Radix Astragali, these four kinds of the most common Chinese medicines of Fructus Lycii have been screened, form new compositions, and determined the proportion that it is suitable, and verified by experiment the effectiveness of its slow down aging skin whitening, the effect that proves simultaneously its slow down aging skin whitening be better than independent employing above-mentioned any one, the effect of two kinds, three kinds Chinese medicines, i.e. the present invention is the synergistic function that has the slow down aging skin whitening between the compositions.
As preferably: its component is by weight: 24 parts of flower of Paeonia lactiflora Pal1., 36 parts on Radix Salviae Miltiorrhizae leaf, 23 parts of the Radixs Astragali, 17 parts of Fructus Lycii.By great many of experiments, obtained the ratio of the best slow down aging skin whitening effect of four kinds of components.
Two of goal of the invention of the present invention is to provide a kind of compositions of safer slow down aging skin whitening.
The technical scheme that adopts is: described flower of Paeonia lactiflora Pal1. removes the wherein flower of Paeonia lactiflora Pal1. of contained benzoic acid processing for process.
As preferably: to be controlled at mass percent be below 0.075% to benzoic acid content in the described flower of Paeonia lactiflora Pal1..
Remove that benzoic method is in the flower of Paeonia lactiflora Pal1.:
Select the flower of Paeonia lactiflora Pal1. when half-open or in full bloom, clean with clear water, then dry in the shade to the degree that petal comes off naturally or a shake-up namely comes off, remove floral disc, pistil, the impurity such as sepal place green-keeping machine with petal, keep the unimpeded of green-keeping machine upper air, lasting 50～70 second moment completes and removes benzoic acid under 285 ℃～315 ℃ temperature, take out, let cool, then petal is put in the baking oven, keep the baking oven upper air unimpeded, under 200 ℃～220 ℃ temperature, toasted 2～3 minutes, further to remove benzoic acid, take out, let cool, put in the baking oven in 102 ℃～104 ℃ temperature dry by the fire to moisture content be 8%～12%, take out, let cool and get final product.
Three of purpose of the present invention is, a kind of preparation method of above-mentioned composition is provided, the technical scheme that adopts is: prepare respectively flower of Paeonia lactiflora Pal1., Radix Salviae Miltiorrhizae leaf, the Radix Astragali and Fructus Lycii, then mix getting final product according to described part by weight, wherein the processing of flower of Paeonia lactiflora Pal1. and preparation are as mentioned above.
The preparation of Radix Salviae Miltiorrhizae leaf:
Select 80～180 days Radix Salviae Miltiorrhizae blade of growth, clean with clear water, spread evenly, soft and the broken degree of blade when holding blade of drying in the shade, blade after will drying in green-keeping machine continues 50～70 second moment and completes under 350 ℃～420 ℃ high temperature, blade after completing is placed on the blade that eases back in the container to be rubbed and not broken degree, till the blade that eases back kneaded the compact bending of nature with kneading machine with blade, put in the baking oven and after toasting 2～3 minutes under 130 ℃～140 ℃ the temperature, pour out, natural storage 20～40 minutes, then after toasting 2～4 minutes under 200 ℃～220 ℃ the temperature, pour out, let cool to room temperature, put in the baking oven to be baked in 50 ℃～60 ℃ temperature and get final product when water content is 10%～13%.
Radix Astragali preparation of sections:
Get the Radix Astragali, clean fast with clear water, place, allow moisture content immerse Radix Astragali inside so that the Radix Astragali is moistening, be cut into the thin slice that thickness is 2～3mm, putting in the baking oven and being baked to water content in 102 ℃～105 ℃ temperature is 8%～12%, and sealing is preserved;
The preparation of Fructus Lycii:
Get Fructus Lycii, clean fast with clear water, place, dry the moisture content of surface adhesion, putting in the baking oven and being baked to water content in 102 ℃～105 ℃ temperature is 10%～14%, and sealing is preserved.
Four of purpose of the present invention is, the application of above-mentioned composition is provided, the technical scheme that adopts is: make tea by flower of Paeonia lactiflora Pal1., Radix Salviae Miltiorrhizae leaf, the Radix Astragali and the Fructus Lycii processed by the method for preparing tea with above, perhaps the Radix Salviae Miltiorrhizae leaf is several with the method for decocting or all use postcritical method to extract respectively active ingredient with all the other flower of Paeonia lactiflora Pal1. of diafiltration, the Radix Astragali and Fructus Lycii, then adds the suitable dosage form such as suitable adjuvant granulation agent, tablet, syrup, oral liquid.
In sum, owing to adopted technique scheme, the invention has the beneficial effects as follows:
At first, adopted four kinds of Chinese medicines as constituent, formed simply, material is easy to obtain, and is with low cost, and obtained the cooperative synergism effect of slow down aging skin whitening;
Secondly, compositions safety of the present invention is controlled, is safe from harm for health.Particularly, on the one hand because material is common Chinese medicine, its contained chemical constituent is clear controlled, on the other hand, adopt simple method to remove in known harmful components benzoic acid, further guaranteed the safety of this compositions of long-term taking, specifically utilizing benzoic acid contained in the flower of Paeonia lactiflora Pal1. is the characteristics that exist take the free state form and in temperature rapid characteristics of distillation during as 100 ℃, adopt simple and convenient, method with low cost removes it, and kept well peoniflorin wherein, Hydroxy peoniflorins etc. have antioxidation, can remove free radical, suppress the composition that lipid peroxide forms, by processed of the present invention, useful composition is easier is dissolved out for these; Again on the one hand, substitute Radix Salviae Miltiorrhizae with the Radix Salviae Miltiorrhizae leaf, both reduced even eliminated the health risk that the heart tonifying composition of Radix Salviae Miltiorrhizae brings, also previously discarded Radix Salviae Miltiorrhizae leaf is effectively utilized, prevented the wasting of resources, produced larger economic benefit and social benefit;
At last, preparation method of composition of the present invention is simple, is convenient to suitability for industrialized production, and with low cost, is easy to promote.
The specific embodiment
Below the present invention is described in detail.
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1: prepare respectively flower of Paeonia lactiflora Pal1., Radix Salviae Miltiorrhizae leaf, the Radix Astragali and Fructus Lycii
The preparation of flower of Paeonia lactiflora Pal1.:
Select flower of Paeonia lactiflora Pal1. in full bloom, clean with clear water, spread evenly, dry in the shade and naturally come off or one touch and namely to come off to petal, remove floral disc, pistil, the impurity such as sepal, petal is put in the green-keeping machine, keep the green-keeping machine upper air unimpeded, lasting 60 second moment completes and removes benzoic acid under 300 ℃ temperature, petal after completing is placed the petal that eases back naturally rubs and not broken degree, the petal that eases back is kneaded compact curling of nature with kneading machine till, the petal after kneading is put in the baking oven, keep the baking oven upper air unimpeded, baking is 2 minutes under 220 ℃ temperature, so that petal molding and further remove benzoic acid, taking-up, let cool, put in the baking oven to be baked in 105 ℃ temperature and get final product when moisture content is 10%.
The preparation of Radix Salviae Miltiorrhizae leaf:
Select 120 days Radix Salviae Miltiorrhizae blade of growth, clean with clear water, spread evenly, soft and the broken degree of blade when holding blade of drying in the shade, blade after will drying in green-keeping machine continues 60 second moment and completes under 385 ℃ high temperature, blade after completing is placed on the blade that eases back in the container to be rubbed and not broken degree, till the blade that eases back kneaded the compact bending of nature with kneading machine with blade, put in the baking oven and pour out after 2 minutes in baking under 135 ℃ the temperature, natural storage 30 minutes, then pour out after 3 minutes in baking under 210 ℃ the temperature, let cool to room temperature, put in the baking oven to be baked in 55 ℃ temperature and get final product when water content is 12%.
Radix Astragali preparation of sections:
Get the Radix Astragali, clean fast with clear water, place, allow moisture content immerse Radix Astragali inside so that the Radix Astragali is moistening, be cut into the thin slice that thickness is 3mm, putting in the baking oven and being baked to water content in 103 ℃ temperature is 10%, and sealing is preserved.
The preparation of Fructus Lycii:
Get Fructus Lycii, clean fast with clear water, place, dry the moisture content of surface adhesion, putting in the baking oven and being baked to water content in 103 ℃ temperature is 12%, and sealing is preserved.
With the above flower of Paeonia lactiflora Pal1. for preparing, Radix Salviae Miltiorrhizae leaf, the Radix Astragali and Fructus Lycii according to the dry product weighing scale, 20 parts of flower of Paeonia lactiflora Pal1., 40 parts on Radix Salviae Miltiorrhizae leaf, 19 parts of the Radixs Astragali, the ratio that Fructus Lycii is 21 parts, mix homogeneously, be distributed into every bag 10 the gram get final product.
Embodiment 2:
A kind of compositions of slow down aging skin whitening, its component is by weight: 20 parts of flower of Paeonia lactiflora Pal1., 40 parts on Radix Salviae Miltiorrhizae leaf, 19 parts of the Radixs Astragali, 21 parts of Fructus Lycii.The preparation method of present embodiment is similar to Example 1, and difference only is not remove benzoic acid.
Embodiment 3: the preparation of integral petal and blade tea
The component of embodiment 2 is evenly mixed, be distributed into every bag of 10g and get final product.
Embodiment 4:
A kind of compositions of slow down aging skin whitening, its component is by weight: 28 parts of flower of Paeonia lactiflora Pal1., 32 parts on Radix Salviae Miltiorrhizae leaf, 27 parts of the Radixs Astragali, 13 parts of Fructus Lycii.The preparation method of present embodiment is similar to Example 1, and difference only is not remove benzoic acid.
Embodiment 5: the flower of Paeonia lactiflora Pal1. and the salvia root leaf tea that are ground into coarse powder
The component of embodiment 1 is ground into respectively coarse powder, and then mix homogeneously is distributed into every bag of 10g and gets final product.
Embodiment 6:
A kind of compositions of slow down aging skin whitening, its component is by weight: 24 parts of flower of Paeonia lactiflora Pal1., 36 parts on Radix Salviae Miltiorrhizae leaf, 23 parts of the Radixs Astragali, 17 parts of Fructus Lycii.The preparation method of present embodiment is similar to Example 1, and difference only is not remove benzoic acid.
Embodiment 7:
A kind of compositions of slow down aging skin whitening, its component is by weight: 24 parts of flower of Paeonia lactiflora Pal1., 36 parts on Radix Salviae Miltiorrhizae leaf, 23 parts of the Radixs Astragali, 17 parts of Fructus Lycii.The method of the preparation method of present embodiment and embodiment 1 is in full accord.
Embodiment 8: the granule that a kind of compositions of slow down aging skin whitening is made
The preparation of flower of Paeonia lactiflora Pal1. extractum:
Select half-open flower of Paeonia lactiflora Pal1., clean with clear water, spread evenly, drying in the shade comes off naturally to petal, removes the impurity such as floral disc, pistil, sepal, and petal is put in the green-keeping machine, keep the green-keeping machine upper air unimpeded, lasting 70 second moment completes and removes benzoic acid under 280 ℃ high temperature, takes out, and lets cool, then petal is put in the baking oven, keep the baking oven upper air unimpeded, baking is 2 minutes under 180 ℃ temperature, further to remove benzoic acid, then pour out, let cool and put in the baking oven that to be baked to moisture content in 106 ℃ temperature be 12%, take out, let cool.
Flower of Paeonia lactiflora Pal1. after getting above oven dry and removing benzoic acid, the decocting that adds 8 times of dry flower weight boiled 1 hour, filtered, and collected filtrate for later use, the decocting that flower of Paeonia lactiflora Pal1. adds 6 times of dry flower weight again boiled 0.5 hour, filter, collect filtrate, with the filtrate merging of front, leave standstill, get supernatant, being concentrated into relative density is 1.18, namely gets flower of Paeonia lactiflora Pal1. extractum.
The preparation of Radix Salviae Miltiorrhizae leaf extractum:
Get Da Ye Radix Salviae Miltiorrhizae leaf, be ground into coarse powder, put in the diafiltration tank, compress, 30～70% the ethanol of getting 5 to 10 times of amounts of Radix Salviae Miltiorrhizae leaf weight adds in the diafiltration tank, till just in time exceeding 1～2 centimetre of Radix Salviae Miltiorrhizae leaf coarse powder to the ethanol liquid level, soak at room temperature 50～100 hours, speed with per minute 1～3ml is emitted percolate by the tank bottoms end of diafiltration, collect for subsequent use, will remain simultaneously ethanol from the top of diafiltration tank adding with the same speed of bottom emitting rate, till adding, continue to collect percolate, till the percolate in the filter tank for seep drains.The percolate of above collection is filtered, filtrate recycling ethanol, being concentrated into relative density is 1.18～1.22, namely gets Radix Salviae Miltiorrhizae leaf extractum.
The preparation of Radix Astragali extractum:
Get the Radix Astragali, get the Radix Astragali, clean fast with clear water, place, allow moisture content immerse Radix Astragali inside so that the Radix Astragali is moistening, be cut into the thin slice that thickness is 2～3mm, the decocting that adds 10 times of astragalus weights boiled 1.5 hours, filtered, and collected filtrate for later use, the decocting that the Radix Astragali adds 7 times of weight again boiled 1 hour, filtered, and collected filtrate, merge with the filtrate of front, leave standstill, get supernatant, being concentrated into relative density is 1.23, namely gets Radix Astragali extractum.
The preparation of Fructus Lycii extractum:
Get Fructus Lycii, clean fast with clear water, the decocting that adds 12 times of Fructus Lycii weight boiled 1.5 hours, filter, collect filtrate for later use, the decocting that Fructus Lycii adds 7 times of weight again boiled 1 hour, filtered, collect filtrate, merge with the filtrate of front, leave standstill, get supernatant, being concentrated into relative density is 1.22, namely gets Fructus Lycii extractum.
Get 1 part of flower of Paeonia lactiflora Pal1. extractum, 1 part of Radix Salviae Miltiorrhizae leaf extractum, 1 part of Radix Astragali extractum, 1 part of Fructus Lycii extractum adds 10 parts of cane sugar powders, granulation, drying is distributed into 10 g/bags, and the lucifuge sealing is preserved and is got final product.
Embodiment 9: the tablet that a kind of compositions of slow down aging skin whitening is made
Adopt embodiment 8 described methods to prepare the extractum of four kinds of compositions, wherein get 1 part of flower of Paeonia lactiflora Pal1. extractum, 1 part of Radix Salviae Miltiorrhizae leaf extractum, 1 part of Radix Astragali extractum, 1 part of Fructus Lycii extractum adds 10 parts of cane sugar powders, granulation, drying is distributed into 10 g/bags, and the lucifuge sealing is preserved and is got final product.
Embodiment 10: the benzoic acid content in the flower of Paeonia lactiflora Pal1. that the method among the embodiment 1 makes is measured:
Measure according to high performance liquid chromatography (" Chinese pharmacopoeia version appendix in 2010 VI D).
Chromatographic condition and system suitability octadecylsilane chemically bonded silica are filler; Take methanol-0.02mol/L ammonium acetate (5:95) as mobile phase; The detection wavelength is 230nm.Number of theoretical plate calculates by the benzoic acid peak should be not less than 2000.
The preparation precision of reference substance solution takes by weighing benzoic acid standard substance 20mg, puts in the 50ml measuring bottle, adds a small amount of dissolve with methanol and is diluted with water to scale, shakes up, and namely gets (every 1ml contains benzoic acid 0.4mg).
The preparation of need testing solution
Get flower of Paeonia lactiflora Pal1. and grind to form coarse powder, precision takes by weighing 1.5g, puts in the 50ml volumetric flask, adds the NaOH solution 0.5ml of 1.0mol/L, adds water 10ml, adds water to scale behind the sonic oscillation 10min in ultrasonic cleaner.
Algoscopy is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
Use above detection method, respectively to the flower of Paeonia lactiflora Pal1. after processing with method of the present invention and untreated flower of Paeonia lactiflora Pal1. each 3 samples, as a result its benzoic content such as following table 1:
Table 1: benzoic acid testing result
Can find out that from above experimental data after processing method of the present invention was processed, the benzoic content in the flower of Paeonia lactiflora Pal1. reduced greatly, substantially is completely removed, and proves that the present invention is feasible.
Embodiment 11: measure Main Ingredients and Appearance peoniflorin, Hydroxy peoniflorin in the flower of Paeonia lactiflora Pal1.
Measure by high performance liquid chromatography (" Chinese pharmacopoeia version appendix in 2010 VI D).
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filler; Take acetonitrile-0.1% phosphoric acid solution (14:86) as mobile phase; The detection wavelength is 230nm.Number of theoretical plate calculates by the peoniflorin peak should be not less than 2000.
The preparation precision of reference substance solution takes by weighing peoniflorin and the Hydroxy peoniflorin reference substance is an amount of, adds methanol and makes the solution that every 1ml contains peoniflorin 60 μ g and Hydroxy peoniflorin 30 μ g, and get final product.
The preparation of need testing solution is got flower of Paeonia lactiflora Pal1. and ground to form coarse powder, gets 0.2g, and is accurately weighed, puts in the 100ml measuring bottle, adds Diluted Alcohol 50ml, supersound process (power 240W, frequency 45kHz) 30 minutes is taken out, and lets cool, and adds methanol to scale, shake up, filter, get subsequent filtrate, and get final product.
Algoscopy is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
Table 2: peoniflorin, Hydroxy peoniflorin testing result
Can be found out by above experimental data, after processing method of the present invention is processed, in the flower of Paeonia lactiflora Pal1. mainly contain the effective constituent peoniflorin, Hydroxy peoniflorin is not affected, and proves that method of the present invention is feasible.
Embodiment 12
The oxidation and removing free radicals activity experiment of compositions of the present invention:
One, the experiment of restraint of tyrosinase activity
1, instrument and reagent
FA2004 electronic analytical balance (upper flat Instr Ltd. of current chart)
UV762 ultraviolet-uisible spectrophotometer (Shanghai analytical tool factory)
Tryrosinase, Vc ethylether, arbutin provide for Chengdu Delai Biology Science Co., Ltd.
2, the preparation of sample
Take by weighing flower of Paeonia lactiflora Pal1. 20g, Radix Salviae Miltiorrhizae leaf 40g, Radix Astragali 19g, Fructus Lycii 21g (flower of Paeonia lactiflora Pal1. is processed through the method for embodiment 1) adds water 1000ml, puts in the flask and heats, keep little and boiled 1.5 hours, filter, collect filtrate for later use, add again water 1000ml, keep little and boiled 1 hour, filter, collect filtrate, with the filtrate merging of front, leave standstill, get supernatant, be concentrated into 200ml, and get final product.
3, method of testing
Getting four test tubes puts on respectively No. 1, No. 2, No. 3, No. 4; Reagent dosage and concentration with reference to table 3, in vitro add respectively at four and respectively to be listed as listed respective reaction liquid, jolting is evenly mixed, in 37 C preheating 10min, then in vitro add respectively tryrosinase solution at each, in 37 C accurate response 5min, be transferred to rapidly in the cuvette, survey absorbance (A) in the 475nm place;
4, the result calculates and statistics
Be calculated as follows the suppression ratio to tryrosinase:
Suppression ratio=[1-(A3-A4)/(A1-A2)] * 100%
In the formula: the absorbance that A1, A2, A3, A4 record in the 475nm place after the reaction in No. 1, No. 2, No. 3, No. 4 four test tubes in the representative table 3 respectively.
Table 3: the composition (unit: ml) of liquid to be measured
Characterize the effect of inhibitor with the inhibitor concentration (IC50) that causes tryrosinase vigor decline 50%.The active testing result of restraint of tyrosinase sees Table 4.
Table 4: each sample suppresses the active testing result of tyrosine
Above result shows that compositions extracting solution of the present invention has stronger tyrosinase activity to suppress ability, and its activity is also suitable with Vc ethylether, arbutin.
Simultaneously, carried out the contrast experiment
The laboratory sample that this contrast experiment adopts is as follows: (ratio is mass ratio), the quality of each sample of employing are all the same
No. 1: flower of Paeonia lactiflora Pal1.; No. 2: the Radix Salviae Miltiorrhizae leaf; No. 3: the Radix Astragali; No. 4: Fructus Lycii; No. 5: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf (1:1); No. 6: flower of Paeonia lactiflora Pal1.+Radix Astragali (1:1); No. 7: flower of Paeonia lactiflora Pal1.+Fructus Lycii (1:1); No. 8: Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1); No. 9: Radix Salviae Miltiorrhizae leaf+Radix Astragali (1:1); No. 10: Fructus Lycii+Radix Astragali (1:1); No. 11: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf+Radix Astragali (1:1:1); No. 12: flower of Paeonia lactiflora Pal1.+Radix Astragali+Fructus Lycii (1:1:1); No. 13: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1:1); No. 14: the Radix Astragali+Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1:1); No. 15: the compositions of embodiment 2; No. 16: the compositions of embodiment 4; No. 17: the compositions of embodiment 7;
Adopt said method to test experimental result such as table 5:
Table 5: each sample suppresses the active testing result of tyrosine (unit: mg/ml)
Two, the removing of DPPH free radical is tested
1. instrument and reagent
UV762 ultraviolet-uisible spectrophotometer (Shanghai analytical tool factory)
FA2004 electronic analytical balance (upper flat Instr Ltd. of current chart)
Free radical (DPPH), Vc ethylether, arbutin provide for Chengdu Delai Biology Science Co., Ltd, and ethanol provides for Kingsoft, Chengdu reagent company limited.
2. the preparation of sample
Take by weighing flower of Paeonia lactiflora Pal1. 20g, Radix Salviae Miltiorrhizae leaf 40g, Radix Astragali 19g, Fructus Lycii 21g (flower of Paeonia lactiflora Pal1. is processed through the method for embodiment 1) adds water 1000ml, puts in the flask and heats, keep little and boiled 1.5 hours, filter, collect filtrate for later use, add again water 1000ml, keep little and boiled 1 hour, filter, collect filtrate, with the filtrate merging of front, leave standstill, get supernatant, be concentrated into 200ml, and get final product.
3. non-oxidizability experiment
3.1 sample is to the removing experiment of DPPH free radical
The preparation of DPPH solution:
Accurately take by weighing DPPH7.88mg, place the 100ml volumetric flask, to scale, namely getting concentration is the DPPH solution of 2 * 10-4mo1/l with anhydrous alcohol solution, and (0～4 ℃) keeps in Dark Place.
The preparation of test liquid:
Get the extracting solution 40ml of compositions of the present invention, place the 200ml volumetric flask, with ethanol dilution to scale.
Assay method:
Extracting sample solution 5ml evenly mixes with 2 * 10-4mo1/l DPPH solution 5ml respectively, places in the dark 30min, measures its absorbance A take methanol at the 525nm place as blank, and calculates its clearance rate with following formula:
Clearance rate (%)=1-(Ai-Aj)/Ac ］ * 100%
In the formula: Ac:5ml ethanol adds the absorbance of 5mlDPPH solution;
Ai:5ml liquid to be measured adds the absorbance of 5mlDPPH solution;
Aj:5ml liquid to be measured adds the absorbance of 5ml ethanol.
Calculate clearance rate according to top formula.
Characterize the effect of free radical scavenger with the activity concentration (IC50) of removing DPPH free radical 50%.The radical scavenging activities testing result sees Table 6.
Table 6: each sample radical scavenging activities testing result
The result shows that compositions extracting solution of the present invention has stronger DPPH radical scavenging activity, and its active and Vc ethylether, arbutin are suitable.
Simultaneously, carried out the contrast experiment
The laboratory sample that this contrast experiment adopts is as follows: (ratio is mass ratio), the quality of each sample of employing are all the same,
No. 1: flower of Paeonia lactiflora Pal1.; No. 2: the Radix Salviae Miltiorrhizae leaf; No. 3: the Radix Astragali; No. 4: Fructus Lycii; No. 5: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf (1:1); No. 6: flower of Paeonia lactiflora Pal1.+Radix Astragali (1:1); No. 7: flower of Paeonia lactiflora Pal1.+Fructus Lycii (1:1); No. 8: Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1); No. 9: Radix Salviae Miltiorrhizae leaf+Radix Astragali (1:1); No. 10: Fructus Lycii+Radix Astragali (1:1); No. 11: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf+Radix Astragali (1:1:1); No. 12: flower of Paeonia lactiflora Pal1.+Radix Astragali+Fructus Lycii (1:1:1); No. 13: flower of Paeonia lactiflora Pal1.+Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1:1); No. 14: the Radix Astragali+Radix Salviae Miltiorrhizae leaf+Fructus Lycii (1:1:1); No. 15: the compositions of embodiment 2; No. 16: the compositions of embodiment 4; No. 17: the compositions of embodiment 7;
Adopt said method to test experimental result such as table 7:
Table 7: each sample suppresses the active testing result of tyrosine (unit: μ g/ml)
Three, melanocyte test
1, instrument and reagent
XSP-2C biological microscope Shanghai permanent magnetic electronic Science and Technology Ltd.
SK2200H ultrasonic cleaner (Shanghai High Kudos Science Instrument Co., Ltd.)
DHP-9052 constant incubator (Shanghai precision instrument company)
Enzyme-linked immunosorbent assay instrument (Shanghai precision instrument company)
LD4-2A centrifuge (Beijing Medical Centrifugal Machine Factory)
UV762 ultraviolet-uisible spectrophotometer (Shanghai analytical tool factory)
FA2004 electronic analytical balance (upper flat Instr Ltd. of current chart)
Tryrosinase, Vc ethylether, arbutin provide for Chengdu Delai Biology Science Co., Ltd.
2, the preparation of sample
Take by weighing flower of Paeonia lactiflora Pal1. 20g, Radix Salviae Miltiorrhizae leaf 40g, Radix Astragali 19g, Fructus Lycii 21g (flower of Paeonia lactiflora Pal1. is processed through the method for embodiment 1) adds water 1000ml, puts in the flask and heats, keep little and boiled 1.5 hours, filter, collect filtrate for later use, add again water 1000ml, keep little and boiled 1 hour, filter, collect filtrate, with the filtrate merging of front, leave standstill, get supernatant, be concentrated into 200ml, and get final product.
3, mice B16 melanocyte is cultivated
Be that the RPMI-1640 of 10% calf serum is cultivated the B16 melanocyte under 37 ℃, the condition of 5%CO2 with containing mass fraction, the cell inoculum concentration is 3 * 106/L, and every 3d passes once generation.
4, the mensuration of cell proliferation rate
Select the B16 melanocyte of exponential phase, use 0.25% trypsinization, adjusting cell concentration is 2 * 104/ml, is inoculated in 96 well culture plates, and every hole 180uL places cultivation 24h in 37 ℃, the incubator of 5%CO2.The Microscopic observation growth conditions is good, adds respectively tested material 20ul/ hole, continues to cultivate 48h.4h adds the MTT30uL/ hole before finishing, and abandons supernatant behind the 4h, adds the DMSO150uL/ hole again, about vibration 10min, measures the absorbance at 475nm wavelength place with enzyme-linked immunosorbent assay instrument.
Test result is three kinds of active matters to almost all unrestraint effects of propagation of melanocyte, and the suppression ratio of on cell proliferation all is lower than 10%, i.e. three kinds of basic no cytotoxicities of active matter.
5, intracellular tyrosine enzyme activity determination
Get the culture bottle that the B16 melanocyte is inoculated in 25 ㎝ 2, inoculum concentration is 2 * 105/bottle, is replaced with the culture medium that is added with tested material behind the cultivation 48h, continues to cultivate 48h, not add active matter in contrast.After 0.25% trypsinization, with the PBS(phosphate buffer of pH6.8) blow and beat into cell suspension, abandon supernatant behind the low-temperature centrifugation, the harvesting agglomerate.The NaTDC that adds 1mL0.5%, ice bath 15min, the cell lysis preparation contains the tryrosinase extracting solution.Get this extracting solution 0.5ml, add 0.3% DOPA solution 0.5ml after 37 ℃ of pre-temperature, 37 ℃ of water-bath 5min measure absorbance with ultraviolet spectrophotometer at the 475nm place.
It is suitable to the suppression ratio of tryrosinase and Vc ethylether, arbutin that test result is compositions extracting solution of the present invention.
Detect by above external activity, find: the whitening of compositions extracting solution of the present invention, activity of fighting against senium are suitable with Vc ethylether, arbutin.
" double-blind method " measure of merit of the present composition:
According to relevant health food check and the requirement of estimating, we have carried out following test:
1, materials and methods
Given the test agent and using method:
Experimenter's former life, diet are constant, and matched group is taken placebo, and the test-meal group is taken the tea (every bag heavy 10g) of making according to the embodiment of the invention 7 described compositionss, the experimenter takes 3 bags for each person every day at random, every bag with 150ml brewed in hot water 3 times, 450ml water altogether, drinking-water waste.The test-meal cycle is 3 months.
Detecting instrument:
Skin microscope (optical instrument factory, Shanghai)
Skin detection analytical system (Guangzhou Pu Tian economy and trade company limited)
Skin elasticity tester (permanent Instr Ltd. is built in Shanghai)
Skinanalysis apparatus (Hong Kong many power company limited)
2, study subject and grouping
Study subject:
Experimenter's inclusive criteria:
Select the age at 28～60 years old, physical condition is good, without obvious brain, the heart, liver, lung, kidney, hematologic disease, without the Long-term taking medicine history, volunteers the crowd that tested assurance cooperates.Tested crowd gets rid of following object: the age below 28 years old or more than 60 years old, gestation or women breast-feeding their children, to the health food allergy sufferers; Intentionally, the serious disease persons such as liver, kidney and hemopoietic system; Take in a short time the article relevant with tested function, have influence on the judgement person to the result.Participating in the case number before the test-meal is 90 examples, after the test-meal effectively the case number be 63 examples (wherein matched group 30 examples, test-meal 32 examples), losing and visiting the case number is 1 example.
Grouping:
Study subject is divided into test-meal group and matched group at random, and two groups all have harmony on sex, age, Diet lifestyle.
3, observation index and analysis
Everyone of test-meal group and matched group measured with skinanalysis apparatus weekly skin brightness changes, the dermal melanin changes of contents, with skin elasticity tester test skin elasticity, with the depth of the wrinkle of skin microscope and skin detection analytical system test skin.
As a result, from study subject was taken second week, the skin of test-meal group can detect the brightness increase, melanin descends, and from 4th week, the skin of test-meal group can detect elasticity to be strengthened, and wrinkle shoals, and matched group does not change.Detect weekly later on, the useful improvement of above indices of the skin of test-meal group, and matched group does not change.Since the 10th week, the variation of the above indices of the skin of test-meal group begins to reduce gradually until can not measure.Evidence compositions of the present invention has skin whitening, the effect of slow down aging.
The invention has the beneficial effects as follows and reach by the inhibitory action that free radical scavenging effect, tyrosinase inhibitory action, melanocyte melanin are produced, test data and human experimentation the result show that compositions of the present invention has the effect of defying age skin-whitening.
Claims (6)
1.
The antidotal compositions of a kind of skin whitening, its component is by weight: 20～28 parts of flower of Paeonia lactiflora Pal1., 32～40 parts on Radix Salviae Miltiorrhizae leaf, 19～27 parts of the Radixs Astragali, 13～21 parts of Fructus Lycii.
2. the antidotal compositions of skin whitening according to claim 1, it is characterized in that: its component is by weight: 24 parts of flower of Paeonia lactiflora Pal1., 36 parts on Radix Salviae Miltiorrhizae leaf, 23 parts of the Radixs Astragali, 17 parts of Fructus Lycii.
3. the antidotal compositions of skin whitening according to claim 1 is characterized in that: the flower of Paeonia lactiflora Pal1. of described flower of Paeonia lactiflora Pal1. for processing through removing contained benzoic acid wherein.
4. the antidotal compositions of skin whitening according to claim 3 is characterized in that in the described flower of Paeonia lactiflora Pal1. that it is below 0.075% that benzoic acid content is controlled at mass percent.
5. the made tea of the antidotal compositions of the described skin whitening of claim 1 to 4 any one.
6. the dosage form made of the antidotal compositions of the described skin whitening of claim 1 to 4 any one and pharmaceutically acceptable excipient.
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| CN103263536A (en) * | 2013-02-04 | 2013-08-28 | 四川逢春制药有限公司 | Skin-whitening freckle-removing anti-aging composite and preparation method and application thereof |
| CN103316150B (en) * | 2013-06-21 | 2015-06-03 | 四川逢春制药有限公司 | Composition for preventing aging and whitening skin as well as preparation method and application thereof |
| CN105326686B (en) * | 2014-04-09 | 2018-02-09 | 广州重生化妆品实业有限公司 | A kind of skin whitening, moisturizing facial treatment mask |
| CN103989164A (en) * | 2014-05-09 | 2014-08-20 | 深圳市皇都御颜养生科技有限责任公司 | Preparation method of anti-aging health food |
| FR3026947B1 (en) * | 2014-10-10 | 2017-12-08 | Basf Beauty Care Solutions France Sas | ACTIVITY DEGLYCATION OF A COMBINATION OF AN EXTRACT OF SALVIA MILTIORRHIZA AND NIACIN AND / OR NIACINAMIDE |
| CN104840380B (en) * | 2015-01-21 | 2018-03-16 | 青岛科技大学 | A kind of natural materials composition and its application in skin-lightening cosmetic |
| CN105169064A (en) * | 2015-10-11 | 2015-12-23 | 淄博齐鼎立专利信息咨询有限公司 | Medicinal preparation for whitening skin |
| CN111329812A (en) * | 2017-11-24 | 2020-06-26 | 郑家伟 | A whitening skin care product containing natural plant extract |
| CN108324647B (en) * | 2018-04-17 | 2021-07-27 | 广州市帝臣日化科技有限公司 | Skin care emulsion and preparation method thereof |
| CN108245469B (en) * | 2018-04-17 | 2021-06-11 | 深圳市中道美生物科技有限公司 | Anti-aging skin care product |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1457818A (en) * | 2002-05-16 | 2003-11-26 | 杨文志 | Poison expelling and astheniz resisting balance pill and its preparing method |
| CN1660408A (en) * | 2003-12-31 | 2005-08-31 | 赵恒� | Medicine to be taken after being mixed in liquor of possessing bearutified and-faced effects |
-
2011
- 2011-11-14 CN CN2011103591419A patent/CN102366478B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1457818A (en) * | 2002-05-16 | 2003-11-26 | 杨文志 | Poison expelling and astheniz resisting balance pill and its preparing method |
| CN1660408A (en) * | 2003-12-31 | 2005-08-31 | 赵恒� | Medicine to be taken after being mixed in liquor of possessing bearutified and-faced effects |
Non-Patent Citations (4)
| Title |
|---|
| 国内抗衰老中药概述;林远凤;《现代医药卫生》;20081015;第24卷(第19期);2927-2928 * |
| 李建喜等.活性氧自由基在动物机体内的生物学作用.《动物医学进展》.2006,第27卷(第10期), |
| 林远凤.国内抗衰老中药概述.《现代医药卫生》.2008,第24卷(第19期), |
| 活性氧自由基在动物机体内的生物学作用;李建喜等;《动物医学进展》;20061020;第27卷(第10期);33-36 * |
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