CN107308226A - A kind of blue or green bur extract and its preparation method and application - Google Patents
A kind of blue or green bur extract and its preparation method and application Download PDFInfo
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- CN107308226A CN107308226A CN201610270265.2A CN201610270265A CN107308226A CN 107308226 A CN107308226 A CN 107308226A CN 201610270265 A CN201610270265 A CN 201610270265A CN 107308226 A CN107308226 A CN 107308226A
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- green bur
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- 239000000284 extract Substances 0.000 title claims abstract description 67
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 122
- 238000010828 elution Methods 0.000 claims abstract description 44
- 239000002904 solvent Substances 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000000287 crude extract Substances 0.000 claims abstract description 20
- 210000002950 fibroblast Anatomy 0.000 claims abstract description 13
- 230000003712 anti-aging effect Effects 0.000 claims abstract description 12
- 239000008367 deionised water Substances 0.000 claims abstract description 11
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 11
- 239000011347 resin Substances 0.000 claims abstract description 10
- 229920005989 resin Polymers 0.000 claims abstract description 10
- 239000002250 absorbent Substances 0.000 claims abstract description 7
- 230000002745 absorbent Effects 0.000 claims abstract description 7
- 239000002537 cosmetic Substances 0.000 claims abstract description 6
- 238000011068 loading method Methods 0.000 claims abstract description 3
- 229930003944 flavone Natural products 0.000 claims description 11
- 235000011949 flavones Nutrition 0.000 claims description 11
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims description 9
- 150000002212 flavone derivatives Chemical class 0.000 claims description 9
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 9
- 230000001737 promoting effect Effects 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 6
- 150000003254 radicals Chemical class 0.000 claims description 4
- 238000002137 ultrasound extraction Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 239000000706 filtrate Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 239000012458 free base Substances 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 239000003921 oil Substances 0.000 abstract description 22
- 230000000694 effects Effects 0.000 abstract description 11
- 230000007760 free radical scavenging Effects 0.000 abstract description 11
- 238000000034 method Methods 0.000 abstract description 9
- 238000005292 vacuum distillation Methods 0.000 abstract description 3
- 239000000419 plant extract Substances 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 23
- 241000727169 Prinsepia utilis Species 0.000 description 14
- 238000002835 absorbance Methods 0.000 description 7
- 235000013399 edible fruits Nutrition 0.000 description 7
- 230000035755 proliferation Effects 0.000 description 6
- 241001092483 Prinsepia Species 0.000 description 5
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 4
- 230000002087 whitening effect Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 235000008997 Prinsepia uniflora Nutrition 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 230000003064 anti-oxidating effect Effects 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 2
- 150000002213 flavones Chemical class 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- VKOBVWXKNCXXDE-UHFFFAOYSA-N icosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCC(O)=O VKOBVWXKNCXXDE-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000001294 propane Substances 0.000 description 2
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 2
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 2
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 2
- 235000005493 rutin Nutrition 0.000 description 2
- 229960004555 rutoside Drugs 0.000 description 2
- 235000010288 sodium nitrite Nutrition 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 1
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- OCZVHBZNPVABKX-UHFFFAOYSA-N 1,1-diphenyl-2-(2,4,6-trinitrophenyl)hydrazine;ethanol Chemical compound CCO.[O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1NN(C=1C=CC=CC=1)C1=CC=CC=C1 OCZVHBZNPVABKX-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 240000000400 Balanites maughamii Species 0.000 description 1
- 208000010445 Chilblains Diseases 0.000 description 1
- 206010008528 Chillblains Diseases 0.000 description 1
- 208000034656 Contusions Diseases 0.000 description 1
- 241000256113 Culicidae Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000009429 Ginkgo biloba extract Substances 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000594592 Lanugo Species 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- 241000632869 Pachira glabra Species 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 240000002834 Paulownia tomentosa Species 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 241001199649 Senegalia erubescens Species 0.000 description 1
- 206010040849 Skin fissures Diseases 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 208000010040 Sprains and Strains Diseases 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 230000001153 anti-wrinkle effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000006214 castanha do maranho Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 description 1
- 235000012734 epicatechin Nutrition 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 210000000630 fibrocyte Anatomy 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 229940068052 ginkgo biloba extract Drugs 0.000 description 1
- 235000020686 ginkgo biloba extract Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 229930013686 lignan Natural products 0.000 description 1
- 150000005692 lignans Chemical class 0.000 description 1
- 235000009408 lignans Nutrition 0.000 description 1
- -1 linoleic acid, leukotrienes Chemical class 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Dermatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Birds (AREA)
- Chemical & Material Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to plant extracts technical field, a kind of blue or green bur extract and its preparation method and application is disclosed.Blue or green bur adds water the preparation method of blue or green bur extract of the present invention or ethanol is extracted, and obtains blue or green bur crude extract；Blue or green bur crude extract concentration, loading is to macroporous absorbent resin, eluted successively with deionized water, 30% ethanol solution, 70% ethanol solution and 95% ethanol solution, vacuum distillation behind each elution position is collected respectively and removes solvent, is correspondingly made available blue or green bur water elution position, 30% ethanol solution elution position, 70% ethanol solution elution position and 95% ethanol solution elution position.The present invention is using blue or green bur as raw material, extracted by proper method and obtain the non-oils extract of blue or green bur, the blue or green bur extract extracted has significant free radical scavenging activity and promotes the effect of human fibroblasts propagation, can be used in skin anti-aging and related cosmetics.
Description
Technical field
The present invention relates to plant extracts technical field, particularly relate to a kind of blue or green bur extract and
Its preparation method and application.
Background technology
Blue or green bur, is rose family Prinsepia Royle (PrinsepiaRoyle) plant Prinsepia utilis
(PrinsepiautilisRoyle) fruit, also known as himalayan prinsepia leaf, gun barrel fruit, milk beat, sponge cake, plum
Flower thorn, bullet fruit, A Nasi, Mengzi Prinsepia utilis.Blue or green bur is mainly distributed on Yunnan, Guizhou, Sichuan
Deng provinces and regions, the contamination-freely band in more than Lijiang, yunnan height above sea level 2000m is abounded with.The fruiting period of blue or green bur is 4~5
Month, ripe fresh blue or green bur aubergine, surface is smooth, and dry fruit is dark brown or black, pericarp shrinkage patch
In on fruit stone, fruit stone is non-breakable, section yellow-white, seed be rich in grease, oil yield 30% or so,
Can be edible and daily.Blue or green bur can be used as medicine, and Naxi of Yunnan Province, Bai nationality, the Yi nationality, distributed over Yunnan, Sichuan and Guizhou, Mo Suo races etc. are few
The applicating history of existing more than one thousand years during number is national.According to modern information, blue or green bur cures mainly many tears of mesh screen,
Indigestion etc..Domestic and international medical personal separates from the plant, to identify flavones ingredient left-handed-
Epicatechin；Lignan component puncture tip alcohol；Fatty acid composition palmitic acid, stearic acid, arachidic acid,
Oleic acid, linoleic acid, leukotrienes etc..In eighties of last century the '20s, American Austria botanist Joseph
Charles Francis doctors Rock are during Lijiang, yunnan is investigated, it may be found that the blue or green bur of magical plant disclose
In generation, and the document saved in Harvard University's Arnold botanical garden then on blue or green bur is recorded and plant
Sample.Prinsepia utilis royle oil is recorded in the Dongba scripture of mystery earliest, in terms of long hair care and skin care
Traditional applicating history and culture, such as prinsepia utilis royle oil is applied on skin, can prevent bite by mosquitos or
The lump of bruises and sprains formation is eliminated, chapped skin, itch is prevented and cures pernio；Wiped with prinsepia utilis royle oil
Hair can suppress epilation, play the role of to make white hair blackening；Human body skin localized burn and scald, are used
Prinsepia utilis royle oil, which is applied to affected part, can prevent wound infection, accelerate wound healing and preventing from scar；Use prinsepia utilis royle oil
The skin of ewborn infant is smeared, makes the dry and comfortable lubrication of skin, and coming off for baby lanugo can be promoted；Blue or green bur
It is referred to as the king of lucky tree and all sorts of flowers.
The research of comprehensive bur blue or green at present, focuses primarily upon the research of prinsepia utilis royle oil, on blue or green bur non-oil
The research of constituents is less, and application of the green grass or young crops bur in terms of cosmetics at present is also main using prinsepia utilis royle oil
Research object.Chinese patent CN104523479A discloses application of the himalayan prinsepia leaf extract in whitening articles for use
And whitening articles for use, it is that the rare research on the non-oil component of blue or green bur is reported, it discloses green grass or young crops thorn
The extract of sharp total polyphenols enrichment, application of the himalayan prinsepia leaf ester alcohol particularly therein in whitening articles for use；Separately
Only one China patent CN103720635A discloses a kind of anti-wrinkle eye cream, and it is with wild paulownia extract, blue or green bur
Extract, Ganodenna Lucidum P.E and ginkgo biloba extract are constituent, wherein blue or green bur extract using propane as
Extraction solvent.But, at the two in the prior art, the former only there is provided the non-oils of blue or green bur and extracts
Application of the thing in terms of whitening, and the latter, using propane as Extraction solvent, what is obtained is the non-pole of prinsepia utilis royle oil class
Property composition, can not come out the larger constituents extraction of flavones isopolarity.Not yet find that blue or green bur is non-at present
The purposes and report of oils extract anti-aging aspect in cosmetic field.
The content of the invention
In view of this, it is an object of the invention to provide a kind of blue or green bur extract and preparation method thereof and should
With, make the blue or green bur extract have significant free radical scavenging activity or/and promote human fibroblasts
The effect of propagation, and then skin ageing can be prevented, the skin state of young healthy is maintained.
For achieving the above object, the present invention provides following technical scheme：
A kind of preparation method of blue or green bur extract, including：
Step 1, blue or green bur add water or ethanol is extracted, and obtains blue or green bur crude extract；
Step 2, blue or green bur crude extract concentration, loading to macroporous absorbent resin, successively with deionized water, 30%
Ethanol solution, 70% ethanol solution and the elution of 95% ethanol solution, collect depressurized behind each elution position respectively
Solvent is distilled off, blue or green bur water elution position, 30% ethanol solution elution position, 70% is correspondingly made available
Ethanol solution elutes position and 95% ethanol solution elution position；
Step 3, the blue or green bur crude extract, which are evaporated, obtains blue or green bur crude extract, blue or green bur water elution position,
30% ethanol solution elution position, 70% ethanol solution elution position, 95% ethanol solution elution position are
Obtained blue or green bur extract.
Blue or green bur extract of the present invention can be any of which or their any kinds
Mixture.
Preferably, the preparation method step 1 is：
Blue or green bur presses 1g:20mL ratios addition deionized water or ethanol, room temperature ultrasonic extraction 2 times, every time
1 hour, centrifugation, supernatant liquid filtering collected all filtrates, obtained blue or green bur crude extract.It is of the present invention
Blue or green bur crude extract corresponds to according to the difference of Extraction solvent and is divided into blue or green bur water crude extract and blue or green bur ethanol
Crude extract, is only blue or green bur crude extract after being evaporated, and correspondence is divided into blue or green bur alcohol extract and blue or green bur water extract.
Preferably, ethanol described in step 1 is 70% ethanol solution.
Preferably, the macroporous absorbent resin is AB-8 type macroporous absorbent resins.
Meanwhile, the present invention also provides the blue or green bur extracted by the invention described above any one preparation method and extracted
Thing, the blue or green bur extract is the blue or green bur extract of non-oils, contains more general flavone, it is preferable that
The blue or green bur extractive total flavone content is 0.04-2.97%.
Preparation method of the present invention is obtained into the survey that blue or green bur extract carries out DPPH method free radical scavenging activities
It is fixed, as a result show that the blue or green bur extract has a higher free radical scavenging activity, and commercially available blue or green bur
The free radical scavenging activity of oil is relatively low, and both compare significant difference；In addition, in anti-aging active detection,
The facilitation bred to human fibroblasts is detected, blue or green bur alcohol extract of the present invention is as a result shown
And its 30% ethanol solution elution position there is the effect for promoting human fibroblasts propagation, and commercially available blue or green thorn
Fruit oil and other extracts have no facilitation.Based on above beneficial effect, the present invention proposes described
Blue or green bur extract in anti-aging and the application in preparing anti-aging cosmetics, also have remove free radical and/
Or promote human fibroblasts propagation, and preparing the free base product of removing and/or promoting human desmocyte thin
Application in born of the same parents' propagation product.
As the concrete mode of above-mentioned application, the invention provides a kind of skin preparations for extenal use of anti-aging, bag
Containing the blue or green bur extract prepared by above-mentioned any one preparation method of the invention.
From above technical scheme, the present invention is extracted by proper method and obtained using blue or green bur as raw material
The blue or green non-oils extract of bur is obtained, the blue or green bur extract extracted has significant free radical scavenging activity
With the effect for promoting human fibroblasts propagation, it can be used in skin anti-aging and related cosmetics.
Embodiment
The embodiment of the invention discloses a kind of blue or green bur extract and its preparation method and application.This area skill
Art personnel can use for reference present disclosure, be suitably modified technological parameter realization.In particular, institute
There is similar replacement and change apparent to those skilled in the art, they are considered as bag
Include in the present invention.The product of the present invention and application are described by preferred embodiment, relevant people
Member can substantially not depart from present invention, product as described herein and method carried out in spirit and scope
Change or suitably change with combining, to realize and apply the technology of the present invention.
For a further understanding of the present invention, a kind of blue or green bur provided with reference to embodiment the present invention carries
Thing and its preparation method and application is taken to be described in detail.
Embodiment 1：The preparation of blue or green bur extract of the present invention
70% ethanol 100mL is added in the commercially available blue or green burs of 50g, in room temperature ultrasonic extraction 2 times, every time 1
Hour, the extract solution that merging is 2 times obtains blue or green bur ethanol crude extract.
Blue or green bur ethanol crude extract is divided into two parts, takes 50ml to be directly evaporated and obtains blue or green bur alcohol extract,
Remaining 50ml is concentrated under reduced pressure into 25ml, with AB-8 resin columns adsorb, then successively with deionized water,
30% ethanol, 70% ethanol and 95% ethanol elution, vacuum distillation removes solvent after collecting respectively, correspondingly
Obtain blue or green bur water elution position A, 30% alcohol elution B, 70% alcohol elution C and 95%
Alcohol elution D；
Blue or green bur alcohol extract obtained above and blue or green bur water elution position A, 30% alcohol elution B,
70% alcohol elution C and 95% alcohol elution D are blue or green bur extract of the present invention.
Embodiment 2：The preparation of blue or green bur extract of the present invention
Deionized water 100mL is added in the commercially available blue or green burs of 50g, in room temperature ultrasonic extraction 2 times, every time 1
Hour, the extract solution that merging is 2 times obtains blue or green bur water crude extract.
Blue or green bur water crude extract is divided into two parts, takes 50ml to be directly evaporated and obtains blue or green bur water extract,
Remaining 50ml is concentrated under reduced pressure into 25ml, with AB-8 resin columns adsorb, then successively with deionized water,
30% ethanol, 70% ethanol and 95% ethanol elution, vacuum distillation removes solvent after collecting respectively, correspondingly
Obtain blue or green bur water elution position E, 30% alcohol elution F, 70% alcohol elution G and 95%
Alcohol elution H；
Blue or green bur water extract obtained above and blue or green bur water elution position E, 30% alcohol elution F,
70% alcohol elution G and 95% alcohol elution H are blue or green bur extract of the present invention.
Embodiment 3：Determination of total flavonoids in blue or green bur extract
Using each blue or green bur extract of embodiment 1 and embodiment 2 as detection object, according to Chinese people's republicanism
State's pharmacopeia (2010 editions) method, using rutin as standard items, determines general flavone content in extract.Determine
Step is：1) standard curve making：Precision measure reference substance solution 1ml, 2ml, 3ml, 4ml, 5ml,
6ml, puts in 25ml measuring bottles respectively, respectively adds 30% ethanol to 6.0ml, plus 5% sodium nitrite solution 1ml.
Mix, place 6 minutes, then add 10% aluminum nitrate solution 1ml, shake up, place 6 minutes.Hydrogenation oxidation
Sodium test solution 10ml, then add 30% ethanol to scale, shake up, place 15 minutes, using corresponding reagent as blank,
With UV-VIS spectrophotometry, absorbance is determined at 510nm wavelength, using absorbance as ordinate,
Concentration is abscissa, draws standard curve.2) a certain amount of sample is taken to be placed in 25ml volumetric flasks, it is other
According to standard curve preparation process, from " plus sodium nitrite solution 1ml ", absorbance is determined in accordance with the law, together
When take need testing solution 3ml, in addition to sodium hydroxide test solution is not added with, remaining is ibid operated, be used as blank, meter
Calculate general flavone content in extract (in terms of rutin).It the results are shown in Table 1.
General flavone content in the blue or green bur extract of table 1
| Sample ID | General flavone content % |
| Blue or green bur alcohol extract | 1.35 |
| Blue or green bur solvent elutes position A | 0.04 |
| Blue or green bur solvent elutes position B | 0.89 |
| Blue or green bur solvent elutes position C | 2.71 |
| Blue or green bur solvent elutes position D | 0.93 |
| Blue or green bur water extract | 1.46 |
| Blue or green bur solvent elutes position E | 0.05 |
| Blue or green bur solvent elutes position F | 0.93 |
| Blue or green bur solvent elutes position G | 2.97 |
| Blue or green bur solvent elutes position H | 1.10 |
As shown in Table 1, blue or green bur extract described in the present invention contains more general flavone, through excessive
After the resin separation of hole, general flavone content is significantly improved, and activity is also remarkably reinforced.
Embodiment 4：The measure (measure of DPPH method free radical scavenging activities) of antioxidation activity
Each blue or green bur extract prepared by embodiment 1 and embodiment 2, its removing is determined with DPPH methods
Free radical activity.Determination step is：Sample is configured to the aqueous solution with deionized water respectively, 2mL is pipetted
In 10mL tool plug test tubes, plus people's 2mL DPPH ethanol solutions (2*10-4Mol/L), fully mix,
It is stored at room temperature, the absorbance A of spectrophotometric determination 517nm wavelength is used after 30min517(using ethanol as ginseng
Than)；2mL DPPH solution and the mixed absorbance A of 2mL ethanol are determined simultaneouslyc, and 2mL carries
Take liquid and the mixed absorbance A of 2mL ethanol solutions0.Parallel determination three times, averages, according to
Lower formula calculates free radical scavenging activity, records a demerit and is shown in Table 2.
Free radical scavenging activity=(1- (A517-A0)/Ac) * 100%.
The measurement result of the DPPH method free radical scavenging activities of table 2
| Sample ID | Sample concentration mg/ml | Free radical scavenging activity % |
| Prinsepia utilis royle oil (commercially available) | 5 | 25 |
| Blue or green bur alcohol extract | 0.05 | 74 |
| Blue or green bur solvent elutes position A | 0.05 | 56 |
| Blue or green bur solvent elutes position B | 0.05 | 88 |
| Blue or green bur solvent elutes position C | 0.05 | 84 |
| Blue or green bur solvent elutes position D | 0.05 | 67 |
| Blue or green bur water extract | 0.05 | 81 |
| Blue or green bur solvent elutes position E | 0.05 | 73 |
| Blue or green bur solvent elutes position F | 0.05 | 96 |
| Blue or green bur solvent elutes position G | 0.05 | 91 |
| Blue or green bur solvent elutes position H | 0.05 | 85 |
As shown in Table 2, heretofore described each blue or green bur extract is respectively provided with excellent radicals scavenging and made
With, can be as antioxidant, and antioxidation activity is significantly larger than commercially available prinsepia utilis royle oil.Therefore, may be used
With by each blue or green bur extract alone or in combination in skin preparations for extenal use, as preventing skin ageing, maintenance
The antioxidation activity thing of the skin state of young healthy is used.
Embodiment 5：The measure of anti-aging active (facilitation of human fibroblasts propagation is determined)
Each blue or green bur extract prepared according to embodiment 1 and embodiment 2 is configured to quality with deionized water
Percentage is 0.01% solution, is added in human fibroblasts nutrient solution, with the deionized water without sample
For blank control.After culture 48 hours, take a part of cell sample, after cell dyeing, use enzyme at random
Mark instrument and determine absorbance at 475nm, assess the effect for promoting human fibroblasts propagation, blank control
Proliferation rate is 100%, if proliferation rate is by promotion proliferation function to cell higher than 100%, is higher than 120%
Proliferation function is remarkably promoted to cell, 3 are the results are shown in Table.
The anti-aging active of table 3 is tested
| Sample ID | Human fibroblasts proliferation rate % |
| Prinsepia utilis royle oil (commercially available) | 99.03±1.69 |
| Blue or green bur alcohol extract | 104±1.37 |
| Blue or green bur solvent elutes position A | 83.3±6.83 |
| Blue or green bur solvent elutes position B | 138.63±3.71 |
| Blue or green bur solvent elutes position C | 96.77±6.08 |
| Blue or green bur solvent elutes position D | 94.63±5.03 |
| Blue or green bur water extract | 97.35±3.14 |
| Blue or green bur solvent elutes position E | 88.11±2.85 |
| Blue or green bur solvent elutes position F | 89.42±5.58 |
| Blue or green bur solvent elutes position G | 98.82±8.71 |
| Blue or green bur solvent elutes position H | 92.25+7.72 |
| Blank control | 100 |
As shown in Table 3, in the present invention blue or green bur alcohol extract and 30% alcohol elution B to people into
The promotion proliferation function that fibrocyte has, wherein the blue or green alcohol elution B of bur 30% promotes propagation to make
It is more notable with effect, and commercially available prinsepia utilis royle oil is contrasted, have no the work for promoting human fibroblasts propagation
With.Therefore, it can by blue or green bur extract coordinate in skin preparations for extenal use, as prevent skin ageing,
The age resister of the skin state of young healthy is maintained to use.
The explanation of above example is only intended to the method and its core concept for helping to understand the present invention.Should
Point out, for those skilled in the art, under the premise without departing from the principles of the invention,
Some improvement and modification can also be carried out to the present invention, these are improved and modification also falls into right of the present invention and wanted
In the protection domain asked.
Claims (9)
1. a kind of preparation method of blue or green bur extract, it is characterised in that including：
Step 1, blue or green bur add water or ethanol is extracted, and obtains blue or green bur crude extract；
Step 2, blue or green bur crude extract concentration, loading to macroporous absorbent resin, successively with deionized water, 30%
Ethanol solution, 70% ethanol solution and the elution of 95% ethanol solution, collect depressurized behind each elution position respectively
Solvent is distilled off, blue or green bur water elution position, 30% ethanol solution elution position, 70% is correspondingly made available
Ethanol solution elutes position and 95% ethanol solution elution position；
Step 3, the blue or green bur crude extract are evaporated the blue or green bur crude extract of acquisition, with blue or green bur water elution position,
30% ethanol solution elution position, 70% ethanol solution elution position, 95% ethanol solution elution position are
Obtained blue or green bur extract.
2. preparation method according to claim 1, it is characterised in that step 1 is：
Blue or green bur presses 1g:20mL ratios addition deionized water or ethanol, room temperature ultrasonic extraction 2 times, every time
1 hour, centrifugation, supernatant liquid filtering collected all filtrates, obtained blue or green bur crude extract.
3. preparation method according to claim 1, it is characterised in that the macroporous absorbent resin is
AB-8 type macroporous absorbent resins.
4. preparation method according to claim 1, it is characterised in that ethanol described in step 1 is 70%
Ethanol solution.
5. blue or green bur extract prepared by preparation method described in claim 1-4 any one.
6. blue or green bur extract according to claim 5, it is characterised in that general flavone content is
0.04-2.97%.
7. application of the blue or green bur extract described in claim 5 in anti-aging and in preparing anti-aging cosmetics.
8. blue or green bur extract described in claim 5 is removing free radical and/or is promoting human fibroblasts to increase
Grow, and preparing the application in removing free base product and/or promoting human fibroblasts propagation product.
9. a kind of skin preparations for extenal use of anti-aging, it is characterised in that include blue or green bur described in claim 5
Extract.
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| CN107955393A (en) * | 2017-11-22 | 2018-04-24 | 云南农业大学 | A kind of extraction process of green grass or young crops bur pigment |
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| CN109222099A (en) * | 2018-10-09 | 2019-01-18 | 昆明理工大学 | The purposes of green bur pericarp and pulp extract |
| CN109364132A (en) * | 2018-12-20 | 2019-02-22 | 文山合丰生物科技有限公司 | The extracting method of effective component and green bur extract gel in tattoo fruit |
| CN114921284A (en) * | 2022-06-16 | 2022-08-19 | 广东丸美生物技术股份有限公司 | Preparation method of prinsepia utilis royle oil and cosmetic |
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| CN107955393A (en) * | 2017-11-22 | 2018-04-24 | 云南农业大学 | A kind of extraction process of green grass or young crops bur pigment |
| CN108553527A (en) * | 2018-06-25 | 2018-09-21 | 昆明贝泰妮生物科技有限公司 | A kind of preparation method of blueness bur benevolence extractive of general flavone |
| CN108553527B (en) * | 2018-06-25 | 2021-08-27 | 云南贝泰妮生物科技集团股份有限公司 | Preparation method of total flavone extract of prinsepia utilis royle |
| CN109222099A (en) * | 2018-10-09 | 2019-01-18 | 昆明理工大学 | The purposes of green bur pericarp and pulp extract |
| CN109364132A (en) * | 2018-12-20 | 2019-02-22 | 文山合丰生物科技有限公司 | The extracting method of effective component and green bur extract gel in tattoo fruit |
| CN109364132B (en) * | 2018-12-20 | 2021-04-27 | 文山合丰生物科技有限公司 | Method for extracting effective components from prinsepia utilis royle |
| CN114921284A (en) * | 2022-06-16 | 2022-08-19 | 广东丸美生物技术股份有限公司 | Preparation method of prinsepia utilis royle oil and cosmetic |
| CN115040445A (en) * | 2022-06-22 | 2022-09-13 | 广东丸美生物技术股份有限公司 | Preparation method of prinsepia utilis royle extract and cosmetics |
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