CN115040445A

CN115040445A - Preparation method of prinsepia utilis royle extract and cosmetics

Info

Publication number: CN115040445A
Application number: CN202210713207.8A
Authority: CN
Inventors: 聂艳峰; 郭朝万; 王丽华; 胡露; 蒲艳; 王娟
Original assignee: Guangdong Marubi Biological Technology Co Ltd
Current assignee: Guangdong Marubi Biological Technology Co Ltd
Priority date: 2022-06-22
Filing date: 2022-06-22
Publication date: 2022-09-13

Abstract

The application relates to the technical field of cosmetics, and relates to a preparation method of prinsepia utilis royle extract and cosmetics. The application provides a preparation method of prinsepia utilis royle extracting solution, which comprises the following steps: extracting Prinsepia utilis Royle by using an extractant solution, and removing an oil-soluble part of the Prinsepia utilis Royle at the upper layer; then adding quaternary ammonium base and lytic enzyme for continuous extraction; centrifuging and taking supernatant; the extractant solution is at least one of polydimethylsiloxane solution, isohexadecane solution, and hexyldecanol solution. Polydimethylsiloxane, isohexadecane solution and hexyldecanol are generally used as emulsifiers in the field, and the application creatively adopts polydimethylsiloxane as an extracting agent to extract the prinsepia utilis royle, so that the effect of emulsification dual-phase extraction can be realized, the oil-soluble part of the prinsepia utilis royle is removed, impurities can be effectively removed, the instability caused by the oil-soluble part is reduced, and the stability of the obtained prinsepia utilis royle extracting solution is good. And effectively promotes the precipitation of flavone and improves the yield of flavone.

Description

Preparation method of prinsepia utilis royle extract and cosmetics

Technical Field

The application relates to the technical field of cosmetics, in particular to a preparation method of a prinsepia utilis royle extract and the cosmetics.

Background

The Prinsepia utilis Royle is fruit of Rosaceae plant semen Panici Miliacei.

The Prinsepia utilis Royle has effects of permeability, cutin softening, moisture keeping, anticracking, antiinflammatory, allergy relieving, ultraviolet resisting, circulation improving, free radical resisting, and peroxidation and aging state improving.

Disclosure of Invention

The embodiment of the application aims to provide a novel preparation method of a prinsepia utilis royle extract and cosmetics, and the obtained prinsepia utilis royle extract is good in stability and high in flavone content.

In a first aspect, the present application provides a method for preparing a prinsepia utilis royle extract, comprising:

extracting Prinsepia utilis Royle with extractant solution, and removing oil-soluble part of Prinsepia utilis Royle; then adding quaternary ammonium base and lytic enzyme for continuous extraction;

centrifuging and taking supernatant;

the extractant solution is at least one of polydimethylsiloxane solution, isohexadecane solution, and hexyldecanol solution.

Polydimethylsiloxane, isohexadecane solution and hexyldecanol are generally used as emulsifiers in the field, and the application creatively adopts polydimethylsiloxane as an extracting agent to extract the prinsepia utilis royle, so that the effect of emulsification dual-phase extraction can be realized, the oil-soluble part of the prinsepia utilis royle is removed, impurities can be effectively removed, the instability caused by the oil-soluble part is reduced, and the stability of the obtained prinsepia utilis royle extracting solution is good. And effectively promotes the precipitation of flavone and improves the yield of flavone.

In other embodiments of the present application, the polydimethylsiloxane solution, the isohexadecane solution, or the hexyldecanol solution all comprise 20% to 50% of the prinsepia utilis royle by volume percentage.

In other embodiments of the present application, the weight of water in the extractant solution is 5 times to 30 times the weight of the prinsepia utilis royle.

In other embodiments of the present application, extracting the prinsepia utilis royle with the extractant solution comprises:

the mixed solution of the prinsepia utilis royle powder and the extractant solution is stirred and extracted for 0.5 to 2.0 hours at the temperature of between 37 and 45 ℃.

In another embodiment of the present application, the step of preparing the mixture of prinsepia utilis royle powder and the extractant solution includes:

adding water into the prinsepia utilis royle powder; and

at least one of polydimethylsiloxane, isohexadecane and hexyldecanol.

In other embodiments herein, the quaternary ammonium base comprises at least one of betaine, berberine, or magnoline.

In another embodiment of the present application, the quaternary ammonium hydroxide is added in an amount of 0.1 to 1.0% by mass.

In another embodiment of the present application, the amount of the lytic enzyme is 0.5 to 1.0% by mass.

In other embodiments of the present application, removing the upper prinsepia utilis royle oil-soluble portion comprises:

centrifuging the mixture of Prinsepia utilis Royle powder and the extractant solution to remove the uppermost turbid liquid.

In a second aspect, the present application provides a cosmetic comprising the extract of prinsepia utilis prepared by the method for preparing the extract of prinsepia utilis according to any one of the preceding methods.

The cosmetics prepared from the prinsepia utilis royle extracting solution can effectively delay the loss of collagen and achieve the anti-wrinkle effect.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the detailed description of the embodiments of the present application provided below is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the present application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

Some embodiments of the present application provide a method for preparing a prinsepia utilis royle extract, comprising the steps of:

and step S1, preparing the raw material to be extracted.

In some embodiments of the present application, Prinsepia utilis Royle is dried in the sun and then pulverized to obtain Prinsepia utilis Royle powder.

Further optionally, in some embodiments of the present application, the particle size of the prinsepia utilis royle powder is crushed to be within a range of 30-60 meshes.

By pulverizing Prinsepia utilis Royle ex Palustre into powder, extraction efficiency can be improved.

And step S2, preparing an extractant solution.

In some embodiments of the present application, the extractant solution is at least one of a polydimethylsiloxane solution, an isohexadecane solution, and a hexyldecanol solution.

At least one of polydimethylsiloxane solution, isohexadecane solution, and hexyldecanol solution can be used as extractant solution

In some embodiments of the present application, the extractant solution is a polydimethylsiloxane solution.

In some embodiments of the present application, the extractant solution is an isohexadecane solution.

In some embodiments of the present application, the extractant solution is a hexyldecanol solution.

Furthermore, dimethyl silicone oil in the chemical state of polydimethylsiloxane has the appearance from colorless and transparent volatile liquid to extremely high-viscosity liquid or silica gel according to the difference of relative molecular mass, is tasteless and high in transparency, and has physiological inertia and good chemical stability.

Polydimethylsiloxane is generally used as an emulsifier in the field, and the polydimethylsiloxane is creatively adopted as an extracting agent to extract the prinsepia utilis royle, so that the emulsification dual-phase extraction effect can be realized, the oil-soluble part of the prinsepia utilis royle is removed, impurities can be effectively removed, the instability caused by the oil-soluble part is reduced, and the unsaturated fatty acid is easy to oxidize and deteriorate.

Further, isohexadecane is a highly branched alkane having a cetane number of 15.

Isohexadecane, which is commonly used as an emollient and solvent in the art, serves to add texture to the product, is insoluble in water, and can act as a barrier to the skin like paraffin wax to prevent water loss. Meanwhile, isohexadecane is easily biodegradable, has no residual feeling, refreshing skin feeling, stable property, silky smooth skin feeling, and is suitable for skin care and sunscreen products. Due to the multi-branched structure, the spreadability is obviously increased, the water retention is good, the skin feel of the product is fresher than that of a product using paraffin oil, the greasy feel of cream and emulsion products is greatly reduced, and the product is particularly suitable for a water-in-oil system and has extremely low chemical reactivity, so that the stability is better and the compatibility is excellent.

According to the method, the prinsepia utilis royle is creatively extracted by taking isohexadecane as an extracting agent, and the effect of emulsification two-phase extraction can be realized, so that the oil-soluble part of the prinsepia utilis royle is removed, impurities can be effectively removed, the instability caused by the oil-soluble part is reduced, and the content of the oil-soluble part of the prinsepia utilis royle can reach 30%.

Further, hexyldecanol is a polyol.

Hexyldecanol is commonly used in the art as a cosmetic oil ingredient or emulsifier.

The prinsepia utilis royle oil-soluble part is removed, impurities can be effectively removed, instability caused by the oil-soluble part is reduced, and the content of the prinsepia utilis royle oil-soluble part can reach 30%.

In some embodiments of the present application, the extractant solution is a mixed solution of a polydimethylsiloxane solution and an isohexadecane solution.

In some embodiments of the present application, the extractant solution is a mixed solution of a polydimethylsiloxane solution and a hexyldecanol solution.

In some embodiments of the present application, the extractant solution is a mixture of a polydimethylsiloxane solution and an isohexadecane solution, a hexyldecanol solution.

In some embodiments of the present application, the polydimethylsiloxane solution, the isohexadecane solution, or the hexyldecanol solution are each present in an amount of 20% to 50% by weight, by volume, of the prinsepia utilis royle.

Further optionally, the polydimethylsiloxane solution, the isohexadecane solution or the hexyldecanol solution are contained in an amount of 21% -49% of the prinsepia utilis royle by weight percentage and volume percentage. Further optionally, the polydimethylsiloxane solution, the isohexadecane solution or the hexyldecanol solution are all 25% -45% of the prinsepia utilis royle by weight percentage and volume percentage.

Illustratively, the polydimethylsiloxane solution, the isohexadecane solution, or the hexyldecanol solution each comprise 26%, 28%, 30%, 32%, 35%, 38%, 40%, 42%, or 43% by weight of the prinsepia utilis royle.

In some embodiments of the present application, the weight of water in the extractant solution is 5 times to 30 times the weight of the prinsepia utilis royle.

Further optionally, the weight of water in the extractant solution is 6-29 times the weight of the prinsepia utilis royle. Further optionally, the weight of water in the extractant solution is 8-25 times the weight of the prinsepia utilis royle.

Illustratively, the weight of water in the extractant solution is 10 times, 12 times, 15 times, 18 times, 20 times, or 24 times the weight of the prinsepia utilis royle.

And step S3, extracting the prinsepia utilis royle fruits by adopting an extractant solution.

Adding the extractant solution prepared in the step S2 to the prinsepia utilis royle powder prepared in the step S1.

In some embodiments of the present application, extracting the prinsepia utilis royle with the extractant solution comprises:

mixing the prinsepia utilis royle powder with the extractant solution prepared in the step S2.

In some embodiments of the present application, the step of preparing the mixture of prinsepia utilis royle powder and the extractant solution comprises:

adding water into the prinsepia utilis royle powder; and

at least one of polydimethylsiloxane, isohexadecane and hexyldecanol.

In other alternative embodiments of the present application, the extractant solution may not be prepared separately. The extraction agent can be added into Prinsepia utilis Royle powder to be mixed for extraction. Illustratively, water is added to the Prinsepia utilis Royle powder, followed by at least one of polydimethylsiloxane, isohexadecane, and hexyldecanol.

And step S4, removing the oil-soluble part of the Prinsepia utilis Royle.

In some embodiments of the present application, the removal of the oil-soluble portion of the Prinsepia utilis Royle from the upper layer can be achieved by centrifuging the mixed solution obtained in the step S3 to remove the uppermost layer of turbid liquid.

By removing the oil-soluble part of the Prinsepia utilis Royle at the upper layer, not only can impurities be effectively removed, but also more importantly, the instability brought by the oil-soluble part can be effectively reduced, so that the stability of the Prinsepia utilis Royle extracting solution is effectively improved.

And step S5, adding quaternary ammonium base and lytic enzyme to continue extraction.

The content of the main active substance flavone in the obtained prinsepia utilis royle extract can be improved by adding the lytic enzyme.

The above-mentioned lytic enzyme may be commercially available.

Further, by adding quaternary ammonium base, the pH of the whole system can be adjusted, thereby improving the dissolving effect of the lytic enzyme.

The pH value of the solution can be adjusted by adding quaternary ammonium base, the polarity of the solvent can be adjusted at the same time, and the solubility of the alcohol-soluble substance can be improved. Can be used for synergism, improving the activity and selectivity of enzyme, reducing the use of organic solvent and reducing the pollution in the production process.

Further, in some embodiments of the present application, the quaternary ammonium base includes at least one of betaine, berberine, or magnoline.

Illustratively, in some embodiments herein, the quaternary ammonium base described above is betaine.

In some embodiments of the present application, the quaternary ammonium base is berberine.

In some embodiments of the present application, the quaternary ammonium hydroxide is magnolia base.

In some embodiments of the present application, the quaternary ammonium base is a mixture of betaine and berberine.

In some embodiments of the present application, the quaternary ammonium hydroxide is a mixture of betaine and magnoline.

In some embodiments of the present application, the quaternary ammonium base is a mixture of berberine and magnoline.

In some embodiments of the present application, the quaternary ammonium base is a mixture of betaine, berberine and magnoline.

Further, in some embodiments herein, the quaternary ammonium hydroxide is added in an amount of 0.1 to 1.0% by mass.

Further optionally, in some embodiments herein, the quaternary ammonium hydroxide is added in an amount of 0.2 to 0.9% by mass.

Further optionally, in some embodiments herein, the quaternary ammonium hydroxide is added in an amount of 0.3 to 0.8% by mass.

Illustratively, in some embodiments herein, the quaternary ammonium hydroxide is added in an amount of 0.4%, 0.5%, 0.6%, 0.7%, or 0.8% by mass.

Further, in some embodiments of the present application, the amount of the lytic enzyme is 0.5 to 1.0% by mass.

Further optionally, in some embodiments of the present application, the amount of lytic enzyme added is 0.55% to 0.95% by mass. Further optionally, in some embodiments of the present application, the amount of lytic enzyme added is 0.6% to 0.9% by mass.

Illustratively, the amount of lytic enzyme added is 0.65%, 0.70%, 0.75%, 0.80%, or 0.85% by mass.

In some embodiments of the present application, adding a quaternary ammonium base, and dissolving the enzyme to continue the extraction comprises:

adding quaternary ammonium hydroxide and lytic enzyme into the mixed solution of the prinsepia utilis royle powder and the extractant solution, and stirring and extracting for 0.5-2.0 hours at 37-45 ℃.

Further optionally, adding quaternary ammonium base and lytic enzyme to continue extracting comprises:

adding quaternary ammonium hydroxide and lytic enzyme into the mixed solution of the prinsepia utilis royle powder and the extractant solution, and stirring and extracting for 0.7 to 1.8 hours at the temperature of between 38 and 43 ℃.

adding quaternary ammonium hydroxide and lytic enzyme into the mixed solution of the prinsepia utilis royle powder and the extractant solution, and stirring and extracting for 0.8-1.6 hours at the temperature of 39-42 ℃.

Illustratively, adding quaternary ammonium base, lysing enzyme and continuing the extraction includes:

adding quaternary ammonium hydroxide and lytic enzyme into the mixture of Prinsepia utilis Royle powder and extractant solution, and extracting at 39 deg.C, 40 deg.C or 41 deg.C under stirring for 0.9 hr, 1.0 hr, 1.2 hr or 1.4 hr.

And step S6, centrifuging, taking the supernatant, and concentrating.

The extraction mixture obtained in step S5 is centrifuged, and the supernatant is collected and concentrated.

The material volume can be reduced by concentrating, and the material is easy to store.

Some embodiments of the present application provide a prinsepia utilis royle extract, which is prepared by the method for preparing the prinsepia utilis royle extract provided in any one of the embodiments.

Some embodiments of the present application provide a cosmetic, which includes the prinsepia utilis royle extract prepared by the preparation method of the prinsepia utilis royle extract provided in any one of the embodiments.

The features and properties of the present application are described in further detail below with reference to examples.

Example 1

Providing a prinsepia utilis royle extracting solution, and preparing the prinsepia utilis royle extracting solution according to the following preparation steps:

(1) taking the Prinsepia utilis Royle, and crushing to obtain Prinsepia utilis Royle seed powder.

(2) Adding water 20 times the weight of the Prinsepia utilis Royle powder in the step (1) and adding 35% (volume ratio) hexyldecanol into the Prinsepia utilis Royle powder.

(3) Centrifuging to remove the uppermost layer of turbid liquid, adding betaine 0.5 wt% of the Prinsepia utilis Royle powder in (1), adding lytic enzyme 0.75 wt% of the Prinsepia utilis Royle powder in (1), stirring at 37 deg.C for 1.0 h.

(4) Centrifuging, collecting supernatant, and concentrating until the weight of the Prinsepia utilis Royle seed powder is 8 times of the weight of the Prinsepia utilis Royle seed powder.

Example 2

(2) Adding water 20 times the weight of the Prinsepia utilis Royle powder in the step (1), and adding polydimethylsiloxane 35% (volume ratio) of the weight of the Prinsepia utilis Royle powder.

(3) Centrifuging to remove the uppermost layer of turbid liquid, adding berberine 0.8% of the weight of the Prinsepia utilis Royle seed powder in (1), adding lytic enzyme 0.8% of the weight of the Prinsepia utilis Royle seed powder in (1), stirring at 37 deg.C for 2.0 h.

(4) Centrifuging, collecting supernatant, and concentrating until the weight of the Prinsepia utilis Royle seed powder is 8 times that of the Prinsepia utilis Royle seed powder.

Example 3

(1) taking Prinsepia utilis Royle, and crushing to obtain Prinsepia utilis Royle seed powder.

(2) Adding water which is 25 times of the weight of the Prinsepia utilis Royle ex Fr et George powder in the step (1), and adding isohexadecane which is 40% (volume ratio) of the weight of the Prinsepia utilis Royle ex Fr et George powder in the step (1).

(3) Centrifuging to remove the uppermost layer of turbid liquid, adding magnoline 0.9 wt% of the seed powder of Prinsepia utilis Royle of (1), adding lytic enzyme 0.5 wt% of the seed powder of Prinsepia utilis Royle of (1), stirring at 37 deg.C for 1.5 h.

Comparative example 1

(2) Adding water 20 times the weight of the Prinsepia utilis Royle powder in the step (1), adding betaine 0.5% of the weight of the Prinsepia utilis Royle powder in the step (1), adding lytic enzyme 0.75% of the weight of the Prinsepia utilis Royle powder in the step (1), stirring at 37 deg.C for 1.5 h.

(3) Centrifuging, collecting supernatant, and concentrating until the weight of the Prinsepia utilis Royle seed powder is 8 times that of the Prinsepia utilis Royle seed powder.

Comparative example 2

(2) Adding water 20 times the weight of the Prinsepia utilis Royle powder in the step (1), and adding hexyldecanol 35% (by volume) of the weight of the Prinsepia utilis Royle powder in the step (1).

(3) Centrifuging to remove the uppermost layer of turbid liquid, adding 0.75 wt% of lytic enzyme of Prinsepia utilis Royle seed powder in (1), stirring at 37 deg.C for 1.0 h.

Comparative example 3

Comparative example 4

(2) Adding water which is 20 times of the weight of the prinsepia utilis royle seed powder in the step (1).

Experimental example 1

The flavone content in the prinsepia utilis royle extract solutions prepared in examples 1 to 3 and comparative examples 1 to 4 were tested.

The total flavone is measured by sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method, samples with the same volume of each example (comparative example) are transferred, added with water to supplement 2.4ml, and then respectively added with 5 percent NaNO ₂ 0.4mL of solution is shaken and mixed evenly and is kept stand for 6 min; then adding 10% Al (NO) ₃ ) ₃ 0.4mL of solution is shaken and mixed evenly and is kept stand for 6 min; then adding 4.0mL of 4% NaOH solution, adding water to a constant volume of 10mL (adding 2.8mL), shaking, mixing, standing for 15min, adjusting to zero with corresponding solvent blank, and measuring absorbance at 510 nm. And drawing a standard curve by taking the concentration of rutin as a horizontal coordinate and taking the absorbance of 510nm as a vertical coordinate.

The flavone yield calculation formula is as follows:

flavone yield (%) ═ determination sample flavone mass (g)/determination sample medicinal material mass (g) × 100

The results are shown in Table 1:

TABLE 1

	Flavone yield (%)
		Example 1	1.21
Example 2	1.18
		Example 3	1.17
Comparative example 1	0.61
		Comparative example 2	0.59
Comparative example 3	0.52
		Comparative example 4	0.39

As can be seen from Table 1, the highest flavone yield of the Prinsepia utilis Royle extract prepared in example 1 was 1.21. While comparative examples 1 are all below 1%. Therefore, the preparation method of the Prinsepia utilis Royle extract can increase the precipitation of flavone in the Prinsepia utilis Royle extract and effectively improve the yield by using emulsification two-phase extraction to remove the oil-soluble part of the Prinsepia utilis Royle.

Experimental example 2:

the extracts of Prinsepia utilis obtained in examples 1 to 3 and comparative examples 1 to 4 were tested for collagenase inhibition.

(1) 50. mu.L of the Prinsepia utilis Royle extract obtained in examples 1 to 3 and comparative examples 1 to 4 was used as 7 samples, and each of the samples was incubated with 20. mu. L1.1U/mL of collagenase solution and 60. mu.L of glycine buffer solution at 37 ℃ for 20 minutes.

(2) To each sample was added 50. mu.L of a 1mmol/L FALGPA solution to initiate the reaction. For the sample blank, glycine buffer was added instead of FALGPA solution. After incubation at 37 ℃ for 30 minutes, the absorbance was measured at 335 nm.

Calculating an inhibition rate:

in the formula:

a0: adding FALGPA without adding sample to be tested

A1: no sample to be tested, no FALGPA

B0: adding the sample to be tested, adding FALGPA

B1: adding the sample to be tested without adding FALGPA

The results are shown in Table 2:

TABLE 2

	Inhibition ratio (%)
		Example 1	95.6
Example 2	94.3
		Example 3	93.9
Comparative example 1	75.3
		Comparative example 2	68.4
Comparative example 3	64.9
		Comparative example 4	41.6

As can be seen from table 2, the highest collagenase inhibition rate of the prinsepia utilis royle extract prepared in example 1 was 95.6%. The rates of inhibition of collagenase in the prinsepia utilis royle extracts prepared in the comparative examples 1-4 were all low, less than 90%. Therefore, the Prinsepia utilis royle extract preparation method disclosed by the application can be used for removing the oil-soluble part of the Prinsepia utilis royle by using emulsification dual-phase extraction, so that the precipitation of collagenase in the Prinsepia utilis royle extract can be increased, and the inhibition rate can be effectively improved.

Collagen is a biopolymer, the main component in the connective tissue of animals, and is also a functional protein with the largest content and the widest distribution in mammals. The collagen can support the skin structure and reduce the generation of wrinkles. The Prinsepia utilis royle extract has the function of strongly inhibiting collagenase, and can delay the loss of collagen.

Therefore, the cosmetics prepared from the prinsepia utilis royle extracting solution can effectively delay the loss of collagen and achieve the anti-wrinkle effect.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A preparation method of a prinsepia utilis royle extracting solution is characterized by comprising the following steps:

centrifuging and taking supernatant;

the extractant solution is at least one of polydimethylsiloxane solution, isohexadecane solution and hexyldecanol solution.

2. The method of producing the prinsepia utilis royle extracting solution according to claim 1,

according to the volume percentage, the content of the polydimethylsiloxane solution, the content of the isohexadecane solution or the content of the hexyldecanol solution are 20-50% of that of the prinsepia utilis royle.

3. The method of producing the prinsepia utilis royle extracting solution according to claim 1,

the weight of water in the extractant solution is 5-30 times of that of the prinsepia utilis royle.

4. The method of producing the prinsepia utilis royle extracting solution according to claim 1,

the step of adding quaternary ammonium base and dissolving enzyme to continue extraction comprises the following steps:

5. The method of producing the Prinsepia utilis Royle extract according to claim 4, wherein the Prinsepia utilis Royle extract is extracted from the Prinsepia utilis Royle extract,

the extraction of the prinsepia utilis royle by adopting the extractant solution comprises the following steps:

adding water into the prinsepia utilis royle powder; and

at least one of polydimethylsiloxane, isohexadecane and hexyldecanol.

6. The method of producing the prinsepia utilis royle extracting solution according to any one of claims 1 to 5,

the quaternary ammonium base comprises at least one of betaine, berberine or magnoline.

7. The method of producing the prinsepia utilis royle extracting solution according to claim 6,

the addition amount of the quaternary ammonium hydroxide is 0.1-1.0% by mass percent.

8. The method of producing the prinsepia utilis royle extracting solution according to claim 1 or 7,

according to the mass percentage, the adding amount of the lytic enzyme is 0.5-1.0%.

9. The method of producing the prinsepia utilis royle extracting solution according to claim 1,

the removing of the oil-soluble part of the Prinsepia utilis Royle comprises the following steps:

10. A cosmetic comprising the extract of prinsepia utilis royle obtained by the method for producing the extract of prinsepia utilis royle according to any one of claims 1 to 9.