CN105641028A - Process for producing total flavonoids of astragalus membranaceus by utilizing alkaliextraction technology - Google Patents
Process for producing total flavonoids of astragalus membranaceus by utilizing alkaliextraction technology Download PDFInfo
- Publication number
- CN105641028A CN105641028A CN201610017088.7A CN201610017088A CN105641028A CN 105641028 A CN105641028 A CN 105641028A CN 201610017088 A CN201610017088 A CN 201610017088A CN 105641028 A CN105641028 A CN 105641028A
- Authority
- CN
- China
- Prior art keywords
- crude extract
- radix astragali
- total flavones
- ethanol
- astragalus membranaceus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention relates to the technical field of astragalus membranaceus pharmaceutical extraction, and particularly relates to a process for producing total flavonoids of astragalus membranaceus by utilizing an alkali extraction technology. The method comprises the following steps: washing, drying and grinding dried radix astragalus membranaceus, adding the ground radix astragalus membranaceus into alkaline hydrolysate, and soaking at constant temperature for 2-3 hours; repeatedly extracting for three times, mixing the extracting liquids, standing and cooling; adding a natural herbal extract clarifying agent KBT-ZTC; standing, performing rotary distillation and concentrating on crude extract to obtain an extract crude product; adding ethanol into the extract crude product, stirring, standing and precipitating; and centrifugally removing supernatant to obtain precipitate; carrying out separation purification technology sequentially through a macroporous adsorption resin column, a silica column, an ODS open column and a medium-pressure column to obtain a pure product total flavonoids of astragalus membranaceus. According to the process, the traditional Chinese herbal clarification technology is introduced based on a conventional water-extraction alcohol-precipitation method, so that high-quality total flavonoids of astragalus membranaceus can be extracted and purified, the extraction yield of the active ingredient in the total flavonoids of astragalus membranaceus can be improved, and the extraction rate of total flavonoids of astragalus membranaceus is 1.3-1.9mg/g by improving the method.
Description
Technical field
The invention belongs to Radix Astragali extract drugs technical field, particularly relate to the technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones.
Background technology
The Radix Astragali is fabaceous, sweet in the mouth, and slightly warm in nature returns lung, spleen channel, there is tonifying Qi and lifting yang, benefit taste consolidating superficial resistance, inducing diuresis to remove edema, promote the production of body fluid nourish blood, expelling pus and toxin by strengthening QI, the effect such as expelling pus and promoting granulation. Radix Astragali total flavones is one of secondary metabolite of formation in Radix Astragali growth course, has various biological activity; In recent years, food safety and health food enjoy the favor of people, and Radix Astragali total flavones is increasingly becoming studied focus. The chemical composition analysis of the Radix Astragali shows to mainly contain flavonoid and soaping agents, and studies and show the regulatory function that total flavones is useful to human physiological metabolism, illustrates bright prospects for its exploitation. Along with the increase of social pressure, the sharply outburst of cardiovascular and cerebrovascular disease, and flavone has suppression tumor, anti-inflammation detumescence, reduces the functions such as blood fat, is therefore valued by the people gradually and studies.
Current water extraction is the traditional method extracting Chinese medicine flavone, and water extraction method is simple, easy to operation, but energy consumption is relatively big, and extracting method is unstable, extracts the flavone purity obtained not high, contains the impurity such as protein, nucleic acid, starch more. The present invention is to solve that the Radix Astragali flavone yield that above-mentioned water extraction extracts is not high, extract the big problem of energy consumption, according in Radix Astragali total flavones major part effective ingredient physicochemical property and in order to save cost, introduce clarifier dedoping step and multiple stage separation adsorption, effectively remove the impurity in ingredient, reduce the energy consumption that extraction process produces because needing pyroprocess, and making extractive technique technological process tend towards stability, method is reproducible.
Summary of the invention
It is an object of the invention to overcome the shortcomings such as existing medicinal extraction Radix Astragali total flavones purity, extraction ratio are low, it is provided that a kind of technique utilizing water to carry technology production Radix Astragali total flavones.
The technical scheme that realizes of the present invention is, a kind of technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones, with the Radix Astragali for raw material, it is characterised in that the method step includes:
1) crude extract obtains: dry astragalus root raw material 1kg, clean, dry, pulverize after, adds 2 ~ 3 times of alkali solution liquids, after constant temperature soaks 2 ~ 3 hours under 40 ~ 50 DEG C of conditions, repeats to extract 3 times, merge alkali solution liquid liquid standing and cool, obtain crude extract;
2) concentration and evaporation: adding natural botanical extraction liquid clarifier KBT-ZTC in the crude extract that step 1) obtains, addition is 5 ~ 10g/L, after standing 20 ~ 30min, rotary evaporation concentrates crude extract, obtains extractum crude product;
3) remove impurity is dissolved: in step 2) the extractum crude product that obtains adds ethanol, stirring extractum dissolves, and staticly settles, and centrifugal segregation supernatant is precipitated thing, and precipitate adds and stands after ethanol is stirred, centrifugal collecting precipitate;
4) macroporous adsorptive resins separation purification: macroporous adsorptive resins on precipitate step 3) obtained, carries out eluting with ethanol water, collects eluent and concentrating under reduced pressure is dried, obtain crude extract I;
5) silica gel column separating purification: step 4) is dried upper silicagel column after crude extract I ethanol obtained dissolves, carries out eluting with the chloroformic solution containing methanol, collect yellow green eluent and air-dry, obtain crude extract II;
6) with the open column separating purification of ODS: crude extract II ethanol step 5) obtained is the open post of upper ODS after dissolving, and carries out eluting with ethanol water, collect yellow green eluent and concentrating under reduced pressure is dried, obtain crude extract III;
7) pressure column separating purification in using: the upper compression leg of crude extract III step 6) obtained, uses ethanol water eluting, starts to collect eluent from going out peak, stops collection, eluent concentrating under reduced pressure drying, obtain crude extract IV after declining to peak;
8) filtering for crystallizing: the crude extract IV step 7) obtained dissolves with methanol, crystallization is carried out with being slowly added to water after membrane filtration, stir while adding and stop stirring to when yellow floccule occurs, stand 20-50min, filter paper filtering, the yellow medicine that will be left on filter paper volatilizes, and collects the yellow medicine after volatilizing both for Radix Astragali total flavones.
As the further qualifications of the present invention, described cleaning is for using clean water 2 ~ 3 times; Described pulverizing is that astragalus membranaceus powder is broken to 50 ~ 100 orders.
As the further qualifications of the present invention, described rotary evaporation concentration is the extractum of 1.0 ~ 1.50 for crude extract is evaporated to water proportion d.
As the further qualifications of the present invention, in described step 3), the addition of ethanol is 2 ~ 5 times of volumes of extractum.
As the further qualifications of the present invention, described alkali solution liquid is the mixed solution of sodium carbonate, sodium sulfite, calcium chloride, sodium carbonate, sodium sulfite, the calcium chloride content respectively 15 ~ 30%, 15 ~ 30%, 10 ~ 40% in leaching solution.
As the further qualifications of the present invention, described natural botanical extraction liquid clarifier KBT-ZTC is the clarifier that You Ke Lier bio tech ltd produces.
As the further restriction of the present invention, in described step 5), the chloroformic solution containing methanol is the chloroformic solution containing 1 ~ 5% methanol.
The ethanol water that ethanol water is 60 ~ 80% as the further restriction of the present invention, in described step 4), step 6), step 7).
As the further restriction of the present invention, the described filter sizes in step 9) is 0.35 ~ 0.5 ��m.
The present invention achieves following substantial advance and prominent effect:
Start with medicinal Astragalis, high-quality Radix Astragali total flavones is extracted through series of processes, on the basis adding alkali and carrying alcohol deposition method, introduced plant Chinese medicine clarification technique realizes the extraction purification of high-quality Radix Astragali total flavones, the clarification dedoping step introduced can efficiently remove the protein in Radix Astragali total flavones, nucleic acid, the macromole impurity such as starch, and in conjunction with macroporous adsorptive resins, silicagel column, the open post of ODS, middle compression leg separating and purifying technology, improve extracts active ingredients productivity in Radix Astragali total flavones, method Radix Astragali total flavones extraction ratio after improvement is 1.3 ~ more than 1.9mg/g, the Radix Astragali total flavones obtained can be used in veterinary clinic, animal immune being regulated, antiviral, the chemoprophylaxis treatments such as antibiosis.
Radix Astragali total flavones of the present invention is admixed in the feed for nursing of animal according to certain ratio, three meals in a day are fed a meal, the immunity of animal can be significantly improved, sickness rate has been reduced to 20.5% from initial 32%, realize the healthy raising of animal, reduce the treatment of antibiotic, hormonal medicaments, it is achieved ecological raising, improve livestock-raising economic worth.
Detailed description of the invention
Following example describe the present invention and utilize alkalinity extraction technology to produce the technique of Radix Astragali total flavones, and these descriptions are not that present invention is further limited, and all reagent used in following example all can be bought by commercial means.
Embodiment 1
The present embodiment is with the dry Radix Astragali for raw material, and preparation method step includes:
1) crude extract obtains: dry astragalus root raw material 1kg, clean, dry, pulverize after add 5 times of water and (clean as by clean water 3 times, pulverize and be broken to 50 orders for astragalus membranaceus powder), add 2 times of alkali solution liquids, under 50 DEG C of conditions, constant temperature leaches alkali solution liquid after soaking 2 hours, repeating to extract 3 times, united extraction liquid stands and cools, and obtains crude extract; Alkali solution liquid is the mixed solution of sodium carbonate, sodium sulfite, calcium chloride, sodium carbonate, sodium sulfite, the calcium chloride content respectively 30%, 15%, 40% in leaching solution.
2) concentration and evaporation: add the natural botanical extraction liquid clarifier KBT-ZTC produced by Ke Lier bio tech ltd in the crude extract that step 1) obtains, addition is 5g/L, after standing 30min, rotary evaporation concentrates crude extract, is the extractum of 1.50 to water proportion d, obtains extractum crude product;
3) remove impurity is dissolved: in step 2) the extractum crude product that obtains adds ethanol, the addition of ethanol is 5 times of volumes of extractum, stirring extractum dissolves, staticly settle, centrifugal segregation supernatant is precipitated thing, precipitate adds and stands after ethanol is stirred, and centrifugal collecting precipitate obtains sterling Radix Astragali total flavones;
4) macroporous adsorptive resins separation purification: macroporous adsorptive resins on precipitate step 3) obtained, carries out eluting with ethanol water, collects eluent and concentrating under reduced pressure is dried, obtain crude extract I;
5) silica gel column separating purification: step 4) is dried upper silicagel column after crude extract I ethanol obtained dissolves, is that the chloroformic solution containing 15% methanol carries out eluting with the chloroformic solution containing methanol, collects yellow green eluent and air-dry, obtain crude extract II;
6) with the open column separating purification of ODS: crude extract II ethanol step 5) obtained is the open post of upper ODS after dissolving, and carries out eluting with ethanol water, collect yellow green eluent and concentrating under reduced pressure is dried, obtain crude extract III;
7) pressure column separating purification in using: the upper compression leg of crude extract III step 6) obtained, uses ethanol water eluting, starts to collect eluent from going out peak, stops collection, eluent concentrating under reduced pressure drying, obtain crude extract IV after declining to peak;
8) f filtering for crystallizing: the crude extract IV step 7) obtained dissolves with methanol, crystallization is carried out with being slowly added to water after membrane filtration, stir while adding and stop stirring to when yellow floccule occurs, stand 20min, filter paper filtering, the yellow medicine that will be left on filter paper volatilizes, and collects the yellow medicine after volatilizing both for Radix Astragali total flavones.
Ethanol water in step 4), step 6), step 7) is the ethanol water of 60%, and the filter sizes in step 8) is 0.4 ��m.
Originally be the extraction ratio of embodiment Radix Astragali total flavones it is 1.3mg/g.
Embodiment 2
1) crude extract obtains: dry astragalus root raw material 1kg, clean, dry, pulverize after add 10 times of water and (clean as by clean water 2 times, pulverize and be broken to 100 orders for astragalus membranaceus powder), add 3 times of alkali solution liquids, under 40 DEG C of conditions, constant temperature leaches alkali solution liquid after soaking 3 hours, repeating to extract 3 times, united extraction liquid stands and cools, and obtains crude extract; Alkali solution liquid is the mixed solution of sodium carbonate, sodium sulfite, calcium chloride, sodium carbonate, sodium sulfite, the calcium chloride content respectively 15%, 30%, 10% in leaching solution.
2) concentration and evaporation: add the natural botanical extraction liquid clarifier KBT-ZTC produced by Ke Lier bio tech ltd in the crude extract that step 1) obtains, addition is 10g/L, after standing 20min, rotary evaporation concentrates crude extract, is the extractum of 1.0 to water proportion d, obtains extractum crude product;
3) remove impurity is dissolved: in step 2) the extractum crude product that obtains adds ethanol, the addition of ethanol is 2 times of volumes of extractum, and stirring extractum dissolves, staticly settle, centrifugal segregation supernatant is precipitated thing, and precipitate adds and stands after ethanol is stirred, centrifugal collecting precipitate;
4) macroporous adsorptive resins separation purification: macroporous adsorptive resins on precipitate step 3) obtained, carries out eluting with ethanol water, collects eluent and concentrating under reduced pressure is dried, obtain crude extract I;
5) silica gel column separating purification: step 4) is dried upper silicagel column after crude extract I ethanol obtained dissolves, is that the chloroformic solution containing 1% methanol carries out eluting with the chloroformic solution containing methanol, collects yellow green eluent and air-dry, obtain crude extract II;
6) with the open column separating purification of ODS: crude extract II ethanol step 5) obtained is the open post of upper ODS after dissolving, and carries out eluting with ethanol water, collect yellow green eluent and concentrating under reduced pressure is dried, obtain crude extract III;
7) pressure column separating purification in using: the upper compression leg of crude extract III step 6) obtained, uses ethanol water eluting, starts to collect eluent from going out peak, stops collection, eluent concentrating under reduced pressure drying, obtain crude extract IV after declining to peak;
8) f filtering for crystallizing: the crude extract IV step 7) obtained dissolves with methanol, crystallization is carried out with being slowly added to water after membrane filtration, stir while adding and stop stirring to when yellow floccule occurs, stand 50min, filter paper filtering, the yellow medicine that will be left on filter paper volatilizes, and collects the yellow medicine after volatilizing both for Radix Astragali total flavones.
Ethanol water in step 4), step 6), step 7) is the ethanol water of 80%. The described filter sizes in step 8) is 0.5 ��m.
Originally be the extraction ratio of embodiment Radix Astragali total flavones it is 1.6mg/g.
Embodiment 3
1) crude extract obtains: dry astragalus root raw material 1kg, clean, dry, pulverize after add 8 times of water and (clean as by clean water 3 times, pulverize and be broken to 75 orders for astragalus membranaceus powder), add 3 times of alkali solution liquids, under 45 DEG C of conditions, constant temperature leaches alkali solution liquid after soaking 3 hours, repeating to extract 3 times, united extraction liquid stands and cools, and obtains crude extract; Alkali solution liquid is the mixed solution of sodium carbonate, sodium sulfite, calcium chloride, sodium carbonate, sodium sulfite, the calcium chloride content respectively 20%, 20%, 30% in leaching solution.
2) concentration and evaporation: add the natural botanical extraction liquid clarifier KBT-ZTC produced by Ke Lier bio tech ltd in the crude extract that step 1) obtains, addition is 7.5g/L, after standing 25min, rotary evaporation concentrates crude extract, is the extractum of 1.20 to water proportion d, obtains extractum crude product;
3) remove impurity is dissolved: in step 2) the extractum crude product that obtains adds ethanol, the addition of ethanol is 3 times of volumes of extractum, and stirring extractum dissolves, staticly settle, centrifugal segregation supernatant is precipitated thing, and precipitate adds and stands after ethanol is stirred, centrifugal collecting precipitate;
4) macroporous adsorptive resins separation purification: macroporous adsorptive resins on precipitate step 3) obtained, carries out eluting with ethanol water, collects eluent and concentrating under reduced pressure is dried, obtain crude extract I;
5) silica gel column separating purification: step 4) is dried upper silicagel column after crude extract I ethanol obtained dissolves, is that the chloroformic solution containing 3% methanol carries out eluting with the chloroformic solution containing methanol, collects yellow green eluent and air-dry, obtain crude extract II;
6) with the open column separating purification of ODS: crude extract II ethanol step 5) obtained is the open post of upper ODS after dissolving, and carries out eluting with ethanol water, collect yellow green eluent and concentrating under reduced pressure is dried, obtain crude extract III;
7) pressure column separating purification in using: the upper compression leg of crude extract III step 6) obtained, uses ethanol water eluting, starts to collect eluent from going out peak, stops collection, eluent concentrating under reduced pressure drying, obtain crude extract IV after declining to peak;
8) f filtering for crystallizing: the crude extract IV step 7) obtained dissolves with methanol, crystallization is carried out with being slowly added to water after membrane filtration, stir while adding and stop stirring to when yellow floccule occurs, stand 30min, filter paper filtering, the yellow medicine that will be left on filter paper volatilizes, and collects the yellow medicine after volatilizing both for Radix Astragali total flavones.
Ethanol water in step 4), step 6), step 7) is the ethanol water of 70%. The described filter sizes in step 8) is 0.35 ��m.
Originally be the extraction ratio of embodiment Radix Astragali total flavones it is 1.9mg/g.
Application Example 1
Radix Astragali total flavones is admixed in the feed for nursing of pig according to certain ratio and (is added 6g Radix Astragali total flavones in the feedstuff of 1kg), three meals in a day are fed a meal, the immunity of animal can be significantly improved, sickness rate has been reduced to 18.5% from initial 30%, realize the healthy raising of animal, reduce the treatment of antibiotic, hormonal medicaments, it is achieved ecological raising, improve livestock-raising economic worth.
Application Example 2
Radix Astragali total flavones is admixed in the feed for nursing of cattle according to certain ratio and (is added 12g Radix Astragali total flavones in the feedstuff of 1kg), three meals in a day are fed a meal, the immunity of animal can be significantly improved, sickness rate has been reduced to 20.5% from initial 32%, realize the healthy raising of animal, reduce the treatment of antibiotic, hormonal medicaments, it is achieved ecological raising, improve livestock-raising economic worth.
The above embodiment of the present invention scheme is only the description of the invention and can not limit the present invention, claim indicates the scope of the present invention, and the scope of the present invention is not pointed out in above-mentioned explanation, therefore, any change in the implication suitable with claims of the present invention and scope, all will be understood that in the scope being included in claims.
The present invention extracts processing staff's long-term work experience accumulation through multidigit Radix Astragali total flavones, and created by creative work, it is in the extraction process of actual Radix Astragali total flavones, the method can obtain Radix Astragali total flavones extraction ratio for for 1.3 ~ more than 1.9mg/g, and the Radix Astragali total flavones obtained can be used in veterinary clinic chemoprophylaxis treatments such as animal immune adjustment, antiviral, antibiosis.
Claims (9)
1. utilize alkalinity extraction technology to produce a technique for Radix Astragali total flavones, with the Radix Astragali for raw material, it is characterised in that the method step includes:
1) crude extract obtains: dry astragalus root raw material 1kg, clean, dry, pulverize after, adds 2 ~ 3 times of alkali solution liquids, under 40 ~ 50 DEG C of conditions, constant temperature leaches alkali solution liquid after soaking 2 ~ 3 hours, repeats to extract 3 times, merges alkali solution liquid liquid standing and cools, obtains crude extract;
2) concentration and evaporation: adding natural botanical extraction liquid clarifier KBT-ZTC in the crude extract that step 1) obtains, addition is 5 ~ 10g/L, after standing 20 ~ 30min, rotary evaporation concentrates crude extract, obtains extractum crude product;
3) remove impurity is dissolved: in step 2) the extractum crude product that obtains adds ethanol, stirring extractum dissolves, and staticly settles, and centrifugal segregation supernatant is precipitated thing, and precipitate adds and stands after ethanol is stirred, centrifugal collecting precipitate;
4) macroporous adsorptive resins separation purification: macroporous adsorptive resins on precipitate step 3) obtained, carries out eluting with ethanol water, collects eluent and concentrating under reduced pressure is dried, obtain crude extract I;
5) silica gel column separating purification: step 4) is dried upper silicagel column after crude extract I ethanol obtained dissolves, carries out eluting with the chloroformic solution containing methanol, collect yellow green eluent and air-dry, obtain crude extract II;
6) with the open column separating purification of ODS: crude extract II ethanol step 5) obtained is the open post of upper ODS after dissolving, and carries out eluting with ethanol water, collect yellow green eluent and concentrating under reduced pressure is dried, obtain crude extract III;
7) pressure column separating purification in using: the upper compression leg of crude extract III step 6) obtained, uses ethanol water eluting, starts to collect eluent from going out peak, stops collection, eluent concentrating under reduced pressure drying, obtain crude extract IV after declining to peak;
8) filtering for crystallizing: the crude extract IV step 7) obtained dissolves with methanol, crystallization is carried out with being slowly added to water after membrane filtration, stir while adding and stop stirring to when yellow floccule occurs, stand 20-50min, filter paper filtering, the yellow medicine that will be left on filter paper volatilizes, and collects the yellow medicine after volatilizing both for Radix Astragali total flavones.
2. the technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones according to claim 1, it is characterised in that described cleaning is for using clean water 2 ~ 3 times; Described pulverizing is that astragalus membranaceus powder is broken to 50 ~ 100 orders.
3. the technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones according to claim 1, it is characterised in that described rotary evaporation concentration is the extractum of 1.0 ~ 1.50 for crude extract is evaporated to water proportion d.
4. the technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones according to claim 1, it is characterised in that in described step 3), the addition of ethanol is 2 ~ 5 times of volumes of extractum.
5. the technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones according to claim 1, it is characterized in that, described alkali solution liquid is the mixed solution of sodium carbonate, sodium sulfite, calcium chloride, sodium carbonate, sodium sulfite, the calcium chloride content respectively 15 ~ 30%, 15 ~ 30%, 10 ~ 40% in leaching solution.
6. the technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones according to claim 1, it is characterised in that described natural botanical extraction liquid clarifier KBT-ZTC is the clarifier that You Ke Lier bio tech ltd produces.
7. the technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones according to claim 1, it is characterised in that in described step 5), the chloroformic solution containing methanol is the chloroformic solution containing 1 ~ 5% methanol.
8. the technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones according to claim 1, it is characterised in that the ethanol water that ethanol water is 60 ~ 80% in described step 4), step 6), step 7).
9. the technique utilizing alkalinity extraction technology to produce Radix Astragali total flavones according to claim 1, it is characterised in that the described filter sizes in step 9) is 0.35 ~ 0.5 ��m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610017088.7A CN105641028A (en) | 2016-01-12 | 2016-01-12 | Process for producing total flavonoids of astragalus membranaceus by utilizing alkaliextraction technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610017088.7A CN105641028A (en) | 2016-01-12 | 2016-01-12 | Process for producing total flavonoids of astragalus membranaceus by utilizing alkaliextraction technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105641028A true CN105641028A (en) | 2016-06-08 |
Family
ID=56484147
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610017088.7A Pending CN105641028A (en) | 2016-01-12 | 2016-01-12 | Process for producing total flavonoids of astragalus membranaceus by utilizing alkaliextraction technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105641028A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115040445A (en) * | 2022-06-22 | 2022-09-13 | 广东丸美生物技术股份有限公司 | Preparation method of prinsepia utilis royle extract and cosmetics |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104958349A (en) * | 2015-06-29 | 2015-10-07 | 兰捷 | Method for extracting high-purity total flavanone from stragalus membranaceus |
-
2016
- 2016-01-12 CN CN201610017088.7A patent/CN105641028A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104958349A (en) * | 2015-06-29 | 2015-10-07 | 兰捷 | Method for extracting high-purity total flavanone from stragalus membranaceus |
Non-Patent Citations (2)
| Title |
|---|
| 区海燕: "黄芪总黄酮的提取分离及其指纹图谱和生物活性的研究", 《中国优秀硕士学位论文全文数据库（电子期刊）》 * |
| 陈玉昆: "《中药提取生产工艺学上册》", 31 March 1992 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115040445A (en) * | 2022-06-22 | 2022-09-13 | 广东丸美生物技术股份有限公司 | Preparation method of prinsepia utilis royle extract and cosmetics |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102816251B (en) | Preparation method of high-activity astragalus polysaccharide and pharmaceutical composition of high-activity astragalus polysaccharide | |
| CN103432193A (en) | Microwave-assisted aqueous two-phase extraction and separation method of kudzu root total flavones | |
| CN112870236B (en) | Flavone effective part of abelmoschus manihot and preparation method and application thereof | |
| CN101991165B (en) | Sweet potato leaf-perilla leaf composite beverage and preparation method thereof | |
| CN104258193A (en) | Drug composition for treating neurasthenia and preventing senile dementia | |
| CN106490608A (en) | A kind of Herba Dendrobii enhancing immunity oral liquid, electuary, capsule, tablet | |
| CN105497109A (en) | Process for producing milkvetch root total flavones through microbial fermentation technology | |
| CN105504079A (en) | Process for producing astragalus polysaccharide by using ultrasonic technology | |
| CN102211980A (en) | Method for preparing resveratrol extract by using waste residue from grape winery as raw material | |
| CN103735633A (en) | Preparation method of instant gardenia powder | |
| CN100451013C (en) | Producing technique for extracting extract products of amentoflavone | |
| CN102416071A (en) | Medicinal composition with effect of treating insomnia, preparation method and application of medicinal composition | |
| CN105399852A (en) | Technology for producing radix astragali polysaccharide by utilization of alkaline process extraction technology | |
| CN106491720A (en) | A kind of method for extracting plant flavone from common fig leaf juice | |
| CN105641028A (en) | Process for producing total flavonoids of astragalus membranaceus by utilizing alkaliextraction technology | |
| CN1806831B (en) | Medicine for treating psoriasis and its preparing process | |
| CN106860489A (en) | A kind of extracting method of myrica rubra leaf polyphenol | |
| CN103059008B (en) | A kind of method simultaneously preparing puerarin and daidzein | |
| CN101879208A (en) | Method for extracting total flavonoids from mung bean shell | |
| CN105535080A (en) | Process for producing total flavonoids of radix astragali with microwave technology | |
| CN102477105A (en) | Method for combined preparation of polysaccharide and flavone by using pumpkin stems and leaves as raw material | |
| CN105440148A (en) | Process for producing astragalus polysaccharide by using water extraction technology | |
| CN107982310A (en) | The preparation method of compound kudzu root flavones preparation | |
| CN106317005A (en) | Method for extracting rose flavones from rose flower residue water | |
| CN106074630A (en) | A kind of Flavones from Ginkgo Leaves extracting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160608 |
|
| WD01 | Invention patent application deemed withdrawn after publication |