CN107982310A - The preparation method of compound kudzu root flavones preparation - Google Patents
The preparation method of compound kudzu root flavones preparation Download PDFInfo
- Publication number
- CN107982310A CN107982310A CN201711139559.2A CN201711139559A CN107982310A CN 107982310 A CN107982310 A CN 107982310A CN 201711139559 A CN201711139559 A CN 201711139559A CN 107982310 A CN107982310 A CN 107982310A
- Authority
- CN
- China
- Prior art keywords
- preparation
- flavones
- kudzu root
- pueraria lobata
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention discloses a kind of preparation method of compound kudzu root flavones preparation, pretreatment, the hot dipping of method including pueraria lobata carry, isolates and purifies and the preparation of preparation.Have the beneficial effect that：Method is simple, during flavones recovery rate it is high, extraction time is short, and the compound kudzu root flavones preparation obtained has excellent anti-fatigue effect；The movement rate of molecule is big during hot dipping carries, and improves flavones to extracellular stripping quantity, and the not mutability, and the flavones in pueraria lobata can be fully dissolved out at such a temperature of the flavones with certain reproducibility.
Description
Technical field
The present invention relates to active components of plants extractive technique field, more particularly, to the preparation side of compound kudzu root flavones preparation
Method.
Background technology
Pueraria lobata be the perennial defoliation liana Pueraria lobota of pulse family dry root, belong to the Ministry of Public Health announcement be both food and medicine
The article of product.The principle active component of pueraria lobata is the isoflavone compounds such as Puerarin, daidzein, daidzin.Flavones is in vivo
The effect such as phosphoric acid and arachidonic metabolism, the phosphorylation of protein, the transfer of calcium ion, the removing of free radical is participated in, it is right
Treatment cardiovascular and cerebrovascular disease, anticancer, diastole smooth muscle, improve blood circulation, relieving the effect of alcohol and detoxifying etc. has direct or indirect effect.Pueraria lobota
Root flavones is a kind of natural biological flavone, and molecular weight is small, can be absorbed rapidly by human body, can be by blood-brain barrier, it should
Had an optimistic view of with prospect by market.Pueraria lobata is averaged flavones content about 2%, flavones content about 3% wherein in Pueraria lobota skin, content of starch about 7%, Pueraria lobota
Flavones content about 1.2% in meat, content of starch about 70%.Therefore, it is always the concern of the industry how to efficiently use kudzu root flavone
Technical problem.
The content of the invention
It is an object of the invention to provide a kind of preparation method of compound kudzu root flavones preparation, method is simple, during it is yellow
The recovery rate of ketone is high, and extraction time is short, and the compound kudzu root flavones preparation of preparation has excellent anti-fatigue effect.
The present invention is directed to the problem of being mentioned in background technology, and the technical solution taken is：The system of compound kudzu root flavones preparation
Preparation Method, comprises the following steps：
The pretreatment of pueraria lobata：Pueraria lobata is taken, is crushed with medicinal herb grinder, petroleum ether is added, soaks 9-11h at 25-30 DEG C, filter,
By filter residue in 52-58 DEG C of dry 13-16h, degreasing pueraria lobata crushed material is obtained, ungrease treatment is carried out to pueraria lobata, avoids lipid to Huang
The influence of ketone purity, improves the quality of flavones, the bioactivity of product is improved, and adds the economic valency of technique
Value；
Hot dipping carries：Extracting degreasing pueraria lobata crushed material, adds ethanol water, refluxing extraction, must extract by solid-liquid ratio 2-3g/50mL
Liquid, under the conditions of being somebody's turn to do, the recovery rate of flavones is high, and the quality of the flavones extracted is good；
Isolate and purify：Leaching liquor filters, and gained flavone extractive is concentrated in vacuo 20-25min at 35-40 DEG C, dry Pueraria lobota
Root chromocor extract, under the conditions of being somebody's turn to do, further increases the purity of flavones in extract, so as to add the anti-tired of the extract
Labor effect；
The preparation of preparation：By Kudzu Flavonoids Extracts, poly- three pear ester -80 and glycerine 1-2/5.7-13.2/25 in mass ratio
Mixing, obtains compound kudzu root flavones preparation, prepared compound kudzu root flavones preparation has excellent anti-fatigue effect.
Preferably, the mass fraction of ethanol water is 65%-85% during hot dipping carries.Under the conditions of being somebody's turn to do, ethanol water
The polarity of solution is weak, is conducive to the dissolution of the less flavone compound of polarity, and the impurity dissolution such as pigment is few in pueraria lobata, increase
The recovery rate of flavones.
Preferably, reflux temperature is 70-90 DEG C during hot dipping carries.Under the conditions of being somebody's turn to do, the movement rate of molecule is big, improves
Flavones is to extracellular stripping quantity, and the not mutability at such a temperature of the flavones with certain reproducibility.
Preferably, return time is 1.5-2.3h during hot dipping carries.Under the conditions of being somebody's turn to do, the flavones in pueraria lobata can be abundant
Dissolution, while the molecular structure of flavones is not influenced.
Compared with prior art, the advantage of the invention is that：The preparation method of compound kudzu root flavones preparation of the present invention includes
Pretreatment, the hot dipping of pueraria lobata carry, isolates and purifies and the preparation of preparation, and method is simple, and the recovery rate of flavones is high, extraction time
Short, the flavones purity obtained is big；The movement rate of molecule is big during hot dipping carries, and improves flavones to extracellular dissolution
Amount, and the not mutability, and the flavones in pueraria lobata can be fully dissolved out at such a temperature of the flavones with certain reproducibility.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
The preparation method of compound kudzu root flavones preparation, comprises the following steps：
1) pretreatment of pueraria lobata：Pueraria lobata is taken, is crushed with medicinal herb grinder, petroleum ether is added, soaks 9h at 25 DEG C, filter, will filter
Slag obtains degreasing pueraria lobata crushed material, carries out ungrease treatment to pueraria lobata, avoid shadow of the lipid to flavones purity in 52 DEG C of dry 13h
Ring, improve the quality of flavones, the bioactivity of product is improved, add the economic value of technique；
2) hot dipping carries：Extracting degreasing pueraria lobata crushed material, the ethanol water that mass fraction is 65% is added by solid-liquid ratio 2g/50mL,
In 70 DEG C of refluxing extraction 1.5h, leaching liquor is obtained, under the conditions of being somebody's turn to do, the recovery rate of flavones is high, and the quality of the flavones extracted is good；
3) isolate and purify：Leaching liquor filters, and gained flavone extractive is concentrated in vacuo 20min at 35 DEG C, dry that pueraria lobata is yellow
Ketone extract, under the conditions of being somebody's turn to do, further increases the purity of flavones in extract, so as to add the antifatigue work(of the extract
Effect；
4) preparation of preparation：Kudzu Flavonoids Extracts, poly- three pear ester -80 and glycerine in mass ratio 1/5.7/25 are mixed,
Compound kudzu root flavones preparation is obtained, prepared compound kudzu root flavones preparation has excellent anti-fatigue effect.
Embodiment 2：
The preparation method of compound kudzu root flavones preparation, comprises the following steps：It is the pretreatment of pueraria lobata first：Pueraria lobata is taken, uses Chinese medicine
Pulverizer crushes, and adds petroleum ether, soaks 11h at 30 DEG C, filters, and by filter residue in 58 DEG C of dry 16h, obtains the crushing of degreasing pueraria lobata
Thing；Carried followed by hot dipping：Extracting degreasing pueraria lobata crushed material, it is water-soluble to add the ethanol that mass fraction is 85% by solid-liquid ratio 3g/50mL
Liquid, in 90 DEG C of refluxing extraction 2.3h；Followed by being to isolate and purify：Leaching liquor is filtered, by gained flavone extractive at 40 DEG C
It is concentrated in vacuo 25min, dry Kudzu Flavonoids Extracts；It is finally the preparation of preparation：By Kudzu Flavonoids Extracts, poly- three pears
Ester -80 and glycerine in mass ratio 2/13.2/25 mix, and obtain compound kudzu root flavones preparation, prepared compound kudzu root flavones
Preparation has excellent anti-fatigue effect.
Embodiment 3：
The preparation method of compound kudzu root flavones preparation, comprises the following steps：
1) pretreatment of pueraria lobata：Pueraria lobata is taken, is crushed with medicinal herb grinder, petroleum ether is added, soaks 10h at 26 DEG C, filter, will filter
Slag obtains degreasing pueraria lobata crushed material, carries out ungrease treatment to pueraria lobata, avoid shadow of the lipid to flavones purity in 54 DEG C of dry 15h
Ring, improve the quality of flavones, the bioactivity of product is improved, add the economic value of technique；
2) hot dipping carries：Extracting degreasing pueraria lobata crushed material, it is water-soluble to add the ethanol that mass fraction is 67% by solid-liquid ratio 2.5g/50mL
Liquid, adds 3, the 5- dichlorobenzoic acids for accounting for degreasing pueraria lobata crushed material quality 0.1% and accounts for degreasing pueraria lobata crushed material quality
0.14% 2- iodo-benzoic acids, in 75 DEG C of refluxing extraction 1.7h, obtain leaching liquor, and under the conditions of being somebody's turn to do, the recovery rate of flavones is high, is extracted
Flavones quality it is good, 3,5- dichlorobenzoic acids, 2- iodo-benzoic acids and the ethanol added has synergistic effect, changes thin
The molecule arranging structure of after birth, makes selectivity add the transmission speed of flavones through being changed into whole-permeable, shorten extraction when
Between, improve the recovery rate of flavones；
3) isolate and purify：Leaching liquor is filtered, gained flavone extractive is concentrated in vacuo 22min at 36 DEG C, dry pueraria lobata
Chromocor extract, under the conditions of being somebody's turn to do, further increases the purity of flavones in extract, so as to add the antifatigue of the extract
Effect；
4) preparation of preparation：Kudzu Flavonoids Extracts, poly- three pear ester -80 and glycerine 1.5/5.9/25 in mass ratio are mixed
Close, obtain compound kudzu root flavones preparation, prepared compound kudzu root flavones preparation has excellent anti-fatigue effect.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail in embodiment described above, it should be understood that the above is only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (6)
1. the preparation method of compound kudzu root flavones preparation, it is characterised in that：It the described method comprises the following steps：
1) pretreatment of pueraria lobata：Pueraria lobata is taken, is crushed with medicinal herb grinder, petroleum ether is added, soaks 9-11h, mistake at 25-30 DEG C
Filter, by filter residue in 52-58 DEG C of dry 13-16h, obtains degreasing pueraria lobata crushed material；
2) hot dipping carries：Extracting degreasing pueraria lobata crushed material, adds ethanol water, refluxing extraction, obtains leaching liquor；
3) isolate and purify：Leaching liquor filters, and gained flavone extractive is concentrated in vacuo, dry Kudzu Flavonoids Extracts；
4) preparation of preparation：By Kudzu Flavonoids Extracts, poly- three pear ester -80 and glycerine 1-2/5.7-13.2/ in mass ratio
25 mixing, obtain compound kudzu root flavones preparation.
2. the preparation method of compound kudzu root flavones preparation according to claim 1, it is characterised in that：Taken off in the step 2)
The solid-liquid ratio of fat pueraria lobata crushed material and ethanol water is 2-3g/50mL.
3. the preparation method of compound kudzu root flavones preparation according to claim 1, it is characterised in that：Second in the step 2)
The mass fraction of alcohol solution is 65%-85%.
4. the preparation method of compound kudzu root flavones preparation according to claim 1, it is characterised in that：Returned in the step 2)
It is 70-90 DEG C to flow temperature.
5. the preparation method of compound kudzu root flavones preparation according to claim 1, it is characterised in that：Returned in the step 2)
The stream time is 1.5-2.3h.
6. the preparation method of compound kudzu root flavones preparation according to claim 1, it is characterised in that：It is true in the step 3)
The temperature of sky concentration is 35-40 DEG C, time 20-25min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711139559.2A CN107982310A (en) | 2017-11-16 | 2017-11-16 | The preparation method of compound kudzu root flavones preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711139559.2A CN107982310A (en) | 2017-11-16 | 2017-11-16 | The preparation method of compound kudzu root flavones preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107982310A true CN107982310A (en) | 2018-05-04 |
Family
ID=62031493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711139559.2A Withdrawn CN107982310A (en) | 2017-11-16 | 2017-11-16 | The preparation method of compound kudzu root flavones preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107982310A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111743157A (en) * | 2020-07-12 | 2020-10-09 | 恩施硒禾生物科技有限公司 | Selenium flavone composition and preparation method thereof |
| CN111848297A (en) * | 2020-07-31 | 2020-10-30 | 陕西科技大学 | Flavonoid insect-proof antibacterial humic acid selenium fertilizer and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1726956A (en) * | 2005-07-27 | 2006-02-01 | 南京海陵中药制药工艺技术研究有限公司 | Extract product of general flavone of kudzuvine root, preparation method and application |
| CN101143005A (en) * | 2007-09-28 | 2008-03-19 | 武汉市石门山天然食品有限责任公司 | Method for preparing instant pueraria flavone powder granule |
| CN101570581A (en) * | 2009-06-10 | 2009-11-04 | 华中农业大学 | Method for comprehensively extracting pueraria polysaccharide and flavone |
| CN105954450A (en) * | 2016-03-23 | 2016-09-21 | 广东药学院 | HPLC fingerprint spectrum of pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla and building method and application of HPLC fingerprint spectrum |
-
2017
- 2017-11-16 CN CN201711139559.2A patent/CN107982310A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1726956A (en) * | 2005-07-27 | 2006-02-01 | 南京海陵中药制药工艺技术研究有限公司 | Extract product of general flavone of kudzuvine root, preparation method and application |
| CN101143005A (en) * | 2007-09-28 | 2008-03-19 | 武汉市石门山天然食品有限责任公司 | Method for preparing instant pueraria flavone powder granule |
| CN101570581A (en) * | 2009-06-10 | 2009-11-04 | 华中农业大学 | Method for comprehensively extracting pueraria polysaccharide and flavone |
| CN105954450A (en) * | 2016-03-23 | 2016-09-21 | 广东药学院 | HPLC fingerprint spectrum of pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla and building method and application of HPLC fingerprint spectrum |
Non-Patent Citations (2)
| Title |
|---|
| 刘强等: "《中药新产品开发》", 31 March 2013, 中国医药科技出版社 * |
| 杨树平等: ""葛渣总黄酮提取及葛根素含量测定"", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111743157A (en) * | 2020-07-12 | 2020-10-09 | 恩施硒禾生物科技有限公司 | Selenium flavone composition and preparation method thereof |
| CN111848297A (en) * | 2020-07-31 | 2020-10-30 | 陕西科技大学 | Flavonoid insect-proof antibacterial humic acid selenium fertilizer and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103172685B (en) | Method for extracting hesperidin and synephrine from immature bitter oranges | |
| CN104667021A (en) | Compound product containing bamboo leaf flavonoid and preparation method thereof | |
| CN102675139A (en) | Method for extracting ceramide from konjak flying powder | |
| CN105713058A (en) | Method for synchronously preparing chlorogenic acid and cynaroside from Lonicera japonica leaves | |
| CN102746362A (en) | Method for extracting refined astragaloside from astragaliradix | |
| CN110448590B (en) | Oriental millettia root extract with sexual function enhancing effect, and preparation method and application thereof | |
| CN103480178A (en) | Method for extracting active ingredients from coreopsis tinctoria by subcritical water | |
| CN107982310A (en) | The preparation method of compound kudzu root flavones preparation | |
| CN103704414B (en) | Herbal tea and preparation method thereof | |
| CN104940280A (en) | Method for extracting total flavones from radix puerariae employing enzyme preparation | |
| CN101223995B (en) | Preparing method for garlic and onion compound essential oil | |
| CN102031116A (en) | New method for preparing rosemary natural antioxidant | |
| CN104211690B (en) | Method for separating and purifying mangiferin from aquilaria sinensis leaves | |
| CN103059008B (en) | A kind of method simultaneously preparing puerarin and daidzein | |
| CN105732762A (en) | Method for extracting perilla oil and ursolic acid from perilla seeds | |
| CN109568214A (en) | A kind of passion fruit shell extract preparation method | |
| CN108450567A (en) | A kind of preparation method of preventing hypertension tea oil | |
| CN106074630A (en) | A kind of Flavones from Ginkgo Leaves extracting method | |
| CN103408619B (en) | A kind of method of comprehensive utilization of sophora bud resource | |
| CN107383152A (en) | The preparation method of golden flower Tea Saponins | |
| CN101623312A (en) | Method for continuously extracting active substances from Tibet inula root | |
| CN106317005A (en) | Method for extracting rose flavones from rose flower residue water | |
| CN106518653A (en) | Membrane extraction process for hydroxycitric acid | |
| CN109908090A (en) | A kind of jasmine flavones instant powder and preparation method thereof | |
| CN108210876A (en) | A kind of ginseng oral liquid of nourishment for vitality and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180504 |
|
| WW01 | Invention patent application withdrawn after publication |