JP5171206B2 - Moisturizer and external preparation for skin - Google Patents

Description

The present invention relates to a moisturizing agent and an external preparation for skin, in which the moisturizing effect is synergistically improved and exhibits a high moisturizing effect. More specifically, the present invention relates to a moisturizing agent and an external preparation for skin in which the moisture retention function of the skin is synergistically improved by using a combination of α-D-glucopyranosylglycerol and an extract of Hermanus ( Rosa rugosa chumb.).

  In order to provide moisturizing ingredients that have been effective in improving skin symptoms such as rough skin, fine lines, and dullness, and have improved skin texture and improved skin transparency, various active ingredients have been searched for and studied. Has been made. In particular, naturally-derived components are known to have various pharmacological and cosmetic effects, and so far, applications of extracts such as plants and fungi to skin external preparations have been studied.

  For example, as a moisturizing agent excellent in moisturizing effect and safety, an extract of Julianidaceae plant (see Patent Document 1), an extract of Brassicaceae Pydium genus plant (see Patent Document 2), an extract of an Asteraceae genus Camelidae plant ( Patent Document 3), Maple plant extract (see Patent Document 4), Hyssop plant extract (see Patent Document 5), Novanoceae plant extract (see Patent Document 6), Amber plant extract (See Patent Document 7) and the like are known.

JP-A-11-209223 JP 2001-39854 A JP 2002-20262 A JP 2003-1113068 A JP 2004-262862 A JP 2006-69939 A JP 2007-77072 A

  Thus, various substances have been applied as moisturizing ingredients so far. However, there are many substances whose effects are not yet known, and the development of an active ingredient having an excellent moisturizing action has been expected.

As a result of studying various substances, the present inventors have found that the moisture retention function of the skin is synergistic by using a combination of α-D-glucopyranosylglycerol and a hermanus ( Rosa rugosa chumb.) Extract. As a result, the present invention has been completed.

That is, the present invention relates to a moisturizing agent containing α-D-glucopyranosylglycerol and hermanus ( Rosa rugosa chumb.) Extract as active ingredients.

Another invention relates to an external preparation for skin, characterized by containing α-D-glucopyranosylglycerol and an extract of Hermanus ( Rosa rugosa Thunb.).

ADVANTAGE OF THE INVENTION According to this invention, the moisturizer which has the outstanding effect can be provided by using (alpha) -D-glucopyranosyl glycerol and a Hermanus ( Rosa rugosa Thunb.) Extract as an active ingredient.

  Combining these moisturizers with external preparations for skin provides excellent effects in improving skin symptoms such as rough skin, fine lines, and dullness, provides various compositions that improve skin texture and enhance skin transparency. can do.

  Α-D-glucopyranosylglycerol used in the present invention includes (2R) -1-O-α-D-glucopyranosylglycerol (Chemical Formula 1) and (2S) -1-O-α-D. -Three components of glucopyranosyl glycerol (chemical formula 2) and 2-O-α-D-glucopyranosyl glycerol (chemical formula 3) are known, and one or a mixture of two or more thereof should be used. Can do.

α−Ｄ−グルコピラノシルグリセロールを得る方法としては、カビ類のα−グルコシダーゼをグリセロール溶液中で糖類の基質に作用させる方法、清酒、味噌、みりん等の醸造
物から抽出、精製する方法、イソマルトース、マルチトールなどを四酢酸鉛や過ヨウ素酸塩でグリコール開裂したものを還元する方法、あるいはＫｏｅｎｉｇｓ−Ｋｎｏｒｒ反応により合成したβ−グルコシドをアノメリデーションした後、β−グルコシダーゼでβ−グルコシドを加水分解する方法などが挙げられるが、カビ類のα−グルコシダーゼをグリセロール溶液中で糖類の基質に作用させる方法が最も効率が良く特に好ましい。

As a method for obtaining α-D-glucopyranosylglycerol, a method of allowing fungal α-glucosidase to act on a saccharide substrate in a glycerol solution, a method of extracting and purifying from a brew such as sake, miso, mirin, A method of reducing glycol cleavage of isomaltose, maltitol, etc. with lead tetraacetate or periodate, or β-glucoside synthesized by Koenigs-Knorr reaction and then β-glucoside with β-glucosidase The method of allowing fungal α-glucosidase to act on a saccharide substrate in a glycerol solution is the most efficient and particularly preferred.

  α-D-Glucopyranosylglycerol can be used as it is, but it can be diluted with water or a polar solvent, decolorized or deodorized within a range without denaturation or decomposition, column chromatography, etc. You may use after performing the fractionation process by. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

On the other hand, Hermanus ( Rosa rugosa Thumb.) Is a plant belonging to the genus Rosaceae, widely growing in the temperate and subarctic regions of eastern Asia, and in Japan it is a deciduous shrub plant that grows a lot in Hokkaido and in coastal sands.

  When using a hermanus, there is no restriction | limiting in particular in the use site | part, Arbitrary parts, such as a root, a stem, a trunk, a leaf, a petal, can be used. A plurality of parts may be used in combination.

  They can be pulverized as they are, but it is preferable to use an extract from those parts.

  For extraction, any part of the genus Hermanus may be used, but for easy use, whole grass, roots, leaves, petals, etc. may be used. In that case, you may make it obtain an extract using a some site | part. Further, two or more kinds of extracts extracted using different solvents may be mixed and used.

  In the extraction, the plant may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization.

  The extraction can be performed by immersing in an arbitrary extraction solvent for a predetermined time. The extraction solvent may be heated as necessary. Alternatively, extraction can be performed using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, stirring or homogenization in an extraction solvent may be performed. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 1 hour to 14 days.

  As an extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether Solvents such as esters; esters such as butyl acetate and ethyl acetate; ketones such as acetone and ethyl methyl ketone can be used. These may be used alone or in combination of any two or more. Saline, phosphate buffer, phosphate buffered saline, and the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical liquids and subcritical liquids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  Hermanus extract with the above solvent can be used as it is, but it can be left to mature for a certain period of time, or it can be used after being concentrated and dried again in water or a polar solvent. You can also Alternatively, it may be used after performing purification treatment such as decolorization, deodorization, desalting, etc., or fractionation treatment by column chromatography or the like, as long as these physiological functions are not impaired. The above extract of Hermanus, its processed product and fractions can be freeze-dried after each treatment and fractionation and dissolved in a solvent before use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

  A moisturizing agent containing α-D-glucopyranosylglycerol and hermanus extract as an active ingredient has an excellent effect on improving skin symptoms such as rough skin, fine lines and dullness, and improves skin texture. And exerts excellent moisturizing action.

  As long as the moisturizing agent of the present invention contains α-D-glucopyranosyl glycerol and a hermanus extract as active ingredients, the form and presence / absence of other ingredients are not limited. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected according to its use and the like, vehicle (excipient), solvent, Other general additives (antioxidants, colorants, dispersants, etc.) can optionally be included.

  α-D-Glucopyranosylglycerol and Hermanus extract have an excellent moisturizing action, and by combining this, it exhibits an excellent effect in improving skin symptoms such as rough skin, fine lines, dullness, Various external preparations for skin can be provided and skin transparency can be improved.

  Here, the external preparation for skin means all external compositions for external use on the skin or hair, such as cosmetics, quasi-drugs, and external medicines.

  The dosage form of the external preparation for skin is arbitrary, and can be provided, for example, as a solubilizing system such as lotion, a dispersion system such as calamine lotion, or an emulsifying system such as cream or emulsion. Further, it can be provided in various dosage forms such as an aerosol form, an ointment, and a poultice filled with a propellant.

  Specifically, various cosmetics such as emulsions, creams, lotions, lotions, packs, cosmetic liquids, cleaning agents, makeup cosmetics, etc .; liquids, ointments, powders, granules, aerosols, patches, poultices, etc. Various forms of cosmetics, quasi drugs, topical medicines, and the like can be exemplified.

  In addition to the extract of α-D-glucopyranosylglycerol and hermanus, the topical preparation for skin includes pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics and cleansing agents as necessary and necessary. Any component that is usually blended in, such as water, oily components, powder, pigments, emulsifiers, solubilizers, gelling agents, detergents, UV absorbers, anti-inflammatory agents, thickeners, pH adjusters, Chelating agents, drugs (medicinal ingredients), fragrances, resins, antibacterial and antifungal agents, antioxidants, alcohols and the like can be appropriately blended. Furthermore, in the range which does not impair the effect of this invention, combined use with another moisturizer is also possible.

  The blending amount of α-D-glucopyranosylglycerol in the external preparation for skin can be adjusted depending on the purpose and the like, but from the viewpoint of the effect and stability, the total amount is 0.01 to 50.0. % By mass is preferable, and more preferably 0.1 to 25.0% by mass.

  The blending amount of the Hermanus extract into the external preparation for skin can be adjusted depending on the purpose and the like, but is preferably 0.001 to 10.0% by mass, more preferably 0. 01 to 5% by mass.

  Hereinafter, production examples of α-D-glucopyranosylglycerol and hermanus extract, test methods for evaluating the moisturizing effect, formulation examples as external preparations for skin, and use tests will be described in more detail. The technical scope is not limited at all by this.

[Method 1 for producing α-D-glucopyranosylglycerol]
Aspergillus niger of 0.125 U / mL (1 U: pH 5.0, 37 ° C., amount of enzyme releasing 1 μmol of p-NP per minute from 5 mM p-NPG) in 1000 mL of an aqueous solution containing 5% by mass of maltose and 35% by mass of glycerol. Α-glucosidase (transglucosidase L-Amano, manufactured by Amano Enzyme), an enzyme derived from the enzyme, was allowed to react for 24 hours under the conditions of 40 ° C. and reaction pH 5.0, and then maltose was continuously added and reacted 10 times. A reaction solution was obtained. When the obtained α-D-glucopyranosylglycerol was confirmed by GC-MS analysis, (2R) -1-O-α-D-glucopyranosylglycerol (Chemical Formula 1), (2S) -1-O -Α-D-glucopyranosylglycerol (Chemical Formula 2), 2-O-α-D-glucopyranosylglycerol (Chemical Formula 3).

[Method 2 for producing α-D-glucopyranosylglycerol]
1000 mL of sake was fractionated by a Shim-pack SCR-101 (N) (7.9 × 300 mm) column (column temperature: 50 ° C., eluent: water, flow rate: 0.6 mL / min), and α-D-gluco Pyranosylglycerol was obtained. When the obtained α-D-glucopyranosylglycerol was confirmed by GC-MS analysis, (2R) -1-O-α-D-glucopyranosylglycerol (Chemical Formula 1), (2S) -1-O -Α-D-glucopyranosylglycerol (Chemical Formula 2), 2-O-α-D-glucopyranosylglycerol (Chemical Formula 3).

[Method 3 for producing α-D-glucopyranosylglycerol]
To 1 mL of 4% maltitol aqueous solution, 10 mL of 2% by mass of periodic acid was added and reacted at room temperature for 4 minutes. After completion of the reaction, barium chloride was added, and the resulting barium periodate precipitate was filtered and removed. Furthermore, after desalting with an ion exchange column, reduction with sodium borohydride was performed, and fractionation purification was performed by activated carbon chromatography and HPLC to obtain 2-O-α-D-glucopyranosylglycerol (Chemical Formula 3). .

[Method 1 of producing hermanus extract]
2.0 kg of a 50 mass% aqueous ethanol solution was added to 100 g of dried pulverized petals of Hermanus ( Rosa rugosa Thunb.), And the mixture was extracted for 2 hours while stirring at room temperature. The extract was collected by filtration, and after removing the solvent, it was freeze-dried to obtain a Hermanus extract.

[Manufacturing method 2 of Hermanus extract]
2.0 kg of a 50 mass% aqueous ethanol solution was added to 100 g of dried ground crumbs of Hermanus grass and extracted for 2 hours with stirring at room temperature. The extract was collected by filtration, and after removing the solvent, it was freeze-dried to obtain a Hermanus extract.

[Method 3 for producing hermanus extract]
100 g of purified water was added to 5 g of dried dried pulverized petals of Hermanus and extracted with an autoclave (120 ° C., 20 minutes). The extract was collected by filtration, and after removing the solvent, it was freeze-dried to obtain a Hermanus extract.

[Method 4 for producing Hermanus extract]
100 g of purified water was added to 5 g of dried and ground Hermanus grass, and the mixture was extracted with an autoclave (120 ° C., 20 minutes). The extract was collected by filtration, and after removing the solvent, it was freeze-dried to obtain a Hermanus extract.

[Manufacturing method 5 of Hermanus extract]
100 g of dried crushed herbaceous grass was put into a supercritical extraction apparatus, and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under an atmospheric pressure of 15 Mpa. The extract was collected to obtain a Hermanus extract.

  Using the above-mentioned α-D-glucopyranosylglycerol and hermanus extract, the moisturizing effect was evaluated. Note that * and ** described in each evaluation result indicate that the significance probability P value in the t-test is less than 5% (P <0.05) with a significance probability of *, and less than 1% significance probability (P <0.01). ) Is represented by **.

<Evaluation of moisturizing effect>
The evaluation of the moisturizing effect of α-D-glucopyranosyl glycerol and the Hamanus extract was performed by the method shown below. As a sample, α-D-glucopyranosylglycerol of Production Method 1 and a Hermanus extract of Production Method 1 were used.

24 μL of the samples shown in Table 1 (Examples and Comparative Examples) were applied to the forearm, 3 × 4 cm ² range, and the keratin moisture content was measured before application, 15, 30, 60, and 120 minutes after application. The keratin water content was measured at 5 points from each application site using SKICON-200 (manufactured by IBI S Co., Ltd.). The average value of the five-point measured values is defined as the keratin moisture content, and the relative values are shown in Table 1 as the keratin moisture content 1 before application.

  From the results shown in Table 2, Example 1 in which α-D-glucopyranosyl glycerol and Hermanus extract are used in combination is Comparative Examples 1 and 2 in which α-D-glucopyranosyl glycerol and Hermanus extract are blended alone. A significant moisturizing effect was observed. From this, it became clear that it has a synergistic moisturizing effect by using (alpha) -D-glucopyranosyl glycerol and a Hermanus extract together.

  Then, the formulation example of the skin external preparation which mix | blended (alpha) -D-glucopyranosyl glycerol obtained by said each manufacturing method and the Hermanus extract is shown.

[Example 2] Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) 1,3-butylene glycol 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 100 (11) Arginine (1% by weight aqueous solution) 20.0
(12) α-D-Glucopyranosylglycerol [Production Method 1] 5.0
(13) Hermanus extract [Production method 1] 3.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification, cooling is started, and (11) to (13) are sequentially added at 50 ° C. and mixed uniformly.

[Example 3] Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 100 (5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) α-D-Glucopyranosylglycerol [Production Method 1] 25.0
(10) Hermanus extract [Production method 2] 0.1
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (10) are sequentially added, and then sufficiently stirred and mixed uniformly.

[Example 4] Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) 1,3-butylene glycol 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Remainder 100 (11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) α-D-Glucopyranosylglycerol [Production Method 1] 5.0
(13) Hermanus extract [Production method 3] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. (11) is added after completion | finish of emulsification, cooling is started, (12), (13) is added at 50 degreeC, and it mixes uniformly.

[Example 5] Cosmetic liquid (1) The balance (mass%) of purified water 100
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (derived from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) α-D-Glucopyranosylglycerol [Production Method 1] 5.0
(17) Hermanus extract [Production method 4] 1.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Further cool to 40 ° C., add (16) and (17), and mix uniformly.

[Example 6] Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) The remainder (3) made into purified water 100 (3) Sodium hydroxide (10 mass% aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) α-D-Glucopyranosylglycerol [Production Method 1] 0.5
(8) Hermanus extract [Production method 1] 0.1
(9) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, (6) to (9) previously mixed are added and stirred and mixed uniformly.

[Example 7] Cleansing fee (1) Squalane 81.0 (mass%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 100 (4) α-D-glucopyranosylglycerol [Production method 1] 0.1
(5) Hermanus extract [Production method 5] 0.01
Manufacturing method: (1) and (2) are uniformly dissolved. To this, (3) to (5) are sequentially added and mixed uniformly.

[Example 8] Face-wash foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 25.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) The balance (8) α-D-glucopyranosylglycerol [Production Method 1] 0.5 with purified water 100
(9) Hermanus extract [Production method 3] 0.1
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) and (9) are added at 40 ° C. and mixed uniformly.

[Example 9] Make-up base cream (1) Squalane 10.2 (% by mass)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) The balance (100) of purified water 100 Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) α-D-Glucopyranosylglycerol [Production Method 1] 1.0
(13) Hermanus extract [Production method 3] 1.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after emulsification, and the components (11) to (13) are added at 40 ° C. and mixed uniformly.

[Example 10] Emulsion foundation (1) Methylpolysiloxane 2.0 (mass%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Remainder water 100 (11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) α-D-Glucopyranosylglycerol [Production Method 1] 3.0
(18) Hermanus extract [Production method 4] 0.1
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the water phase components (7) to (10) are mixed, dissolved by heating at 75 ° C., the pigments (11) to (15) are added thereto, and the mixture is uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and the components (16) to (18) are sequentially added at 40 ° C. and mixed uniformly.

[Example 11] Water-in-oil emollient cream (1) Liquid paraffin 34.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) α-D-Glucopyranosylglycerol [Production Method 1] 3.0
(11) Hermanus extract [Production method 1] 1.0
(12) Balance of purified water 100 (13) Fragrance 0.1
Manufacturing method: (5) and (6) are dissolved in a part of (12) to 50 ° C., and gradually added to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (11) are added to the remainder of (12) with heating and dissolution at 70 ° C. while stirring, and emulsified with a homomixer. Cooling is started after completion of emulsification, and (13) is added at 40 ° C. and mixed uniformly.

[Example 12] Pack (1) The balance (mass%) of purified water 100
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 9.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) α-D-Glucopyranosylglycerol [Production Method 1] 5.0
(7) Hermanus extract [Production method 3] 0.5
(8) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C., add (6) to (8) and mix uniformly.

[Example 13] Bath agent (1) Fragrance 0.3 (mass%)
(2) α-D-Glucopyranosylglycerol [Production Method 1] 1.0
(3) Hermanus extract [Production method 4] 0.1
(4) Sodium bicarbonate 50.0
(5) Remainder manufacturing method which makes sodium sulfate 100: (1)-(5) is mixed uniformly.

[Example 14] Hair wax (1) Stearic acid 3.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 100 (11) α-D-glucopyranosylglycerol [Production Method 1] 1.0
(12) Hermanus extract [Production method 1] 0.1
(13) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after emulsification, and the components (11) to (13) are added at 40 ° C. and mixed uniformly.

[Example 15] Hair tonic (1) Ethanol 50.0 (mass%)
(2) Purified water 100 (3) α-D-glucopyranosylglycerol [Production Method 1] 1.0
(4) Hermanus extract [Production method 2] 0.01
(5) Fragrance 0.1
Production method: Components (1) to (5) are mixed and homogenized.

<Use test 1>
A use test using the external preparation for skin of Examples 2 and 4 was performed, and the moisture retention of the external preparation for skin containing α-D-glucopyranosylglycerol and a hermanus extract was evaluated. At that time, the total amount of α-D-glucopyranosyl glycerol and hermanus extract blended in Example 2 was replaced with α-D-glucopyranosyl glycerol alone blended as Comparative Example 3, Hermanus extract alone What was changed to the blending was Comparative Example 4, and the total amount of α-D-glucopyranosylglycerol blended in Example 4 and the Hermanus extract was replaced with the blending of α-D-glucopyranosylglycerol alone. A comparative test was conducted in the same manner as Comparative Example 6 except that Comparative Example 5 was replaced with the Hermanus extract alone.

  A group of 15 male and female panelists were allowed to use each sample blindly for one month, and evaluated in three stages: “feel”, “cannot say”, and “do not feel”. Table 3 shows the number of panelists that obtained each evaluation.

  From Table 3, the example use group using α-D-glucopyranosyl glycerol and the hermanus extract in combination is clearer than the comparative example use group containing α-D-glucopyranosyl glycerol and hermanus extract alone. It was found that a good moisturizing property was felt. From this, it became clear that the skin external preparation which mix | blended (alpha) -D-glucopyranosyl glycerol and the Hermanus extract has the outstanding moisturizing effect.

<Use test 2>
The use test using the skin external preparations of Examples 2 and 4 and Comparative Examples 3, 4, 5, and 6 was performed, and the improvement effect of these symptoms was evaluated with respect to rough skin, skin texture, and skin transparency.

  For each sample, a group of 20 male and female panelists in their 20s to 50s who are worried about rough skin, skin texture, and skin transparency are used for 1 month each blind, and changes in skin condition before and after use. Observed and evaluated. As indicators of skin condition, rough skin, skin texture, and skin transparency were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. The rough skin and the transparency of the skin were visually evaluated, and the texture of the skin was observed using a microscope. Table 4 shows the number of panelists that obtained each evaluation.

  From Table 4, the example use group using α-D-glucopyranosylglycerol and the hamanas extract together is clearer than the comparative example use group containing α-D-glucopyranosylglycerol and the hamanas extract alone. Improvement of skin roughness, skin texture and skin transparency was observed. From this, it was found that the external preparation for skin containing α-D-glucopyranosylglycerol and hermanus extract has an excellent effect on improving skin symptoms in rough skin, skin texture, and skin transparency. .

Claims (2)

A moisturizing agent containing α-D-glucopyranosylglycerol and an extract of Hermanus ( Rosa rugosa chumb.) as active ingredients. An external preparation for skin, comprising α-D-glucopyranosylglycerol and an extract of Hermanus ( Rosa rugosa Thunb.).