KR20230052700A - Anti-pollution cosmetic composition containing camellia extract, aurea helianthus extract, carpinus tschonoskii extract, silkworm extract and centella asiatica extract - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a Camellia japonica extract, an Aurea helianthus extract, a Carpinus tschonoskii extract, a silkworm extract, and an Asiatic pennywort extract as active ingredients, and an anti-pollution cosmetic composition with excellent antioxidant and anti-wrinkle effects. The anti-pollution cosmetic composition of the present invention comprises a Camellia japonica extract, an Aurea helianthus extract, a Carpinus tschonoskii extract, a silkworm extract, and an Asiatic pennywort extract as active ingredients and thus can cause minor skin irritation and can provide an anti-pollution effect against contaminants, thereby protecting the skin. The anti-pollution cosmetic composition of the present invention has excellent antioxidant and anti-wrinkle effects, thereby exhibiting the excellent activity of scavenging active oxygen that causes skin troubles, inhibiting the activity of collagen-decomposing enzymes that cause skin wrinkles, and increasing the expression level of collagen.

Description

Anti-Pollution Cosmetic Composition Containing Camellia Extract, Geumhwagyu Extract, Gaseo Fish Extract, Silkworm Extract and Centella Asiatica Extract as Active Ingredients EXTRACT}

The present invention relates to a cosmetic composition containing camellia extract, geumhwagyu extract, fish extract, silkworm extract and centella asiatica extract as active ingredients, and an anti-pollution cosmetic composition with excellent antioxidant and anti-wrinkle effects.

Particulate Matter (PM) refers to small dust with a diameter of 10 μm or less floating in the air, and causes various diseases such as respiratory and skin diseases. It is an air pollutant that includes sulfur dioxide, nitrogen oxides, lead, nitrogen dioxide, ozone, and carbon monoxide, and is generated from automobiles, factories, and cooking processes.

When exposed to fine dust for a long time, immunity is rapidly lowered, and respiratory diseases such as colds, asthma, and bronchitis, as well as cardiovascular diseases, skin diseases, and eye diseases may be exposed to various diseases. Heavy metals such as mercury, lead, and cadmium contained in fine dust and first-class carcinogens such as dioxin and black carbon cause various skin inflammations when they come into direct contact with the skin.

Fine dust has a greater impact on health as the particle size is smaller, and in particular, dust with a diameter of 2.5 μm or less is called ultrafine dust. Ultrafine dust, which is smaller than pores, has very small particles and sticks well, so it penetrates deeply into pores and causes various troubles, causing dermatitis such as atopy. Fine dust that penetrates the skin produces active oxygen in mitochondria, which damages mitochondria, reducing collagen activity and increasing decomposition, causing skin aging.

As it is revealed that fine dust has a serious effect on skin aging, various anti-pollution cosmetics are being developed to protect the skin from fine dust. Anti-pollution cosmetics are products that protect the skin from harmful environments, and are cosmetics that block or remove harmful ingredients. It has the effect of protecting the skin from harmful environments before going out and removing the remaining fine dust from the skin after going out.

Accordingly, the present invention is intended to provide an anti-pollution cosmetic composition containing extracts of natural substances as an active ingredient to reduce skin irritation, protecting the skin from contaminants such as fine dust, and having excellent antioxidant and anti-wrinkle effects.

The present invention is to provide an anti-pollution cosmetic composition having excellent antioxidant and anti-wrinkle effects, containing camellia extract, geumhwagyu extract, sage extract, silkworm extract and centella asiatica extract as active ingredients.

The anti-pollution cosmetic composition of the present invention contains camellia extract, geumhwagyu extract, fish extract, silkworm extract and centella asiatica extract as active ingredients, and may be characterized by having antioxidant and anti-wrinkle effects.

The manufacturing method of the anti-pollution cosmetic composition of the present invention includes: ① pulverizing the prepared camellia flower with a grinder; ② extracting by adding an extraction solvent to the pulverized camellia flower, and filtering through a filter; ③ concentrating the filtered solution in step ② to prepare a powdered camellia extract; ④ Proceeding the above ① to ③ processes with Geumhwagyu flowers to prepare Geumhwagyu extract; ⑤ Proceeding the above steps ① to ③ with resora leaves to prepare an extract of resosa; ⑥ Proceeding the above steps ② to ③ with silkworm powder to produce silkworm extract; ⑦ Proceeding the above steps ① to ③ with centella asiatica to prepare centella asiatica extract; and ⑧ mixing the camellia extract, geumhwagyu extract, fish extract, silkworm extract, and centella asiatica extract at a predetermined ratio, wherein the extraction solvent is water, alcohol having 1 to 10 carbon atoms, and a mixture thereof. At least one selected from, and the camellia extract, geumhwagyu extract, fish extract, silkworm extract, and centella asiatica extract may be blended in a ratio of 3: 2: 3: 1: 1.

The anti-pollution cosmetic composition of the present invention contains camellia extract, geumhwagyu extract, fish extract, silkworm extract and centella asiatica extract as active ingredients, so it has less skin irritation and anti-pollution effect against pollutants such as fine dust. Provides protection for the skin.

The anti-pollution cosmetic composition of the present invention has excellent anti-oxidation and anti-wrinkle effects, so it is excellent in scavenging active oxygen that causes skin trouble, inhibits the activity of collagenase that causes skin wrinkles, and reduces the amount of collagen expression. has an increasing effect.

1 is a graph showing the results of the antioxidant test (DPPH assay) of the present invention.
Figure 2 is a graph showing the DPPH radical scavenging ability evaluation results of the present invention.
Figure 3 is a graph showing the cytotoxicity evaluation results of the present invention.
Figure 4 is a graph showing the results of analysis of skin fibroblast wrinkle factor expression of the present invention.

Below, the present invention will be described in more detail through specific details for carrying out the present invention, <Examples> and <Test Examples>, but the embodiments of the present invention can be modified into various other forms, so the The scope is not limited to the embodiments described below.

Throughout the specification of the present invention, when a certain component is said to "include", it means that it may further include other components, not excluding other components unless otherwise stated. Throughout the specification of the present invention, when a step is said to be located "on" or "before" another step, this means that a step is in a direct time-series relationship with another step, as well as the mixing step after each step. As such, the order of the two steps may include the same rights as in the case of an indirect time-series relationship in which the time-series order may change.

The terms "about", "substantially", etc., of degrees used throughout the specification of the present invention are used at or approximating that value when manufacturing and material tolerances inherent in the stated meaning are given, and the present invention Accurate or absolute figures are used to prevent unfair use by unscrupulous infringers of the disclosed disclosures mentioned for the sake of understanding. The term "step of (doing)" or "step of" used throughout the present specification does not mean "step for".

The anti-pollution cosmetic composition of the present invention is characterized in that it contains camellia extract, geumhwagyu extract, fish extract, silkworm extract and centella asiatica extract as active ingredients.

Camellia is an evergreen broad-leaved arboreous tree of the tea tree family native to China, Japan and Korea. It is said that the name camellia came from the fact that it blooms in winter. It is known that flowers, leaves, and fruits all contain many useful and medicinal ingredients, so there is nothing to throw away.

Plants and flowers are used for ornamental purposes, fruits are used for medicinal purposes or oil is squeezed to make hair oil and lamp oil, and wood is used as a craft material. Seeds are collected from camellia fruits to extract camellia oil, which contains useful components such as oleic acid. Since it does not evaporate well, does not go rancid, has little odor, and is harmless to the human body, it has been used for various purposes since ancient times. It is famous for being used as a hair oil for women, and is also used to prevent corrosion of metal or wooden objects.

Aurea Helianthus is a perennial herbaceous plant belonging to the genus Autumn Sunflower in the Malvaceae family, and is a medicinal plant that is beneficial to health from stem to leaf and root as well as flowers. It contains flavone, vitamin E, hyperoside, plant estrogen, unsaturated fatty acid, ursolic acid, vegetable collagen, phenol compound, lectin, etc. Including anti-melanin effect, it has excellent effects on reducing blood fat, improving diabetes, and improving vascular diseases. Geumhwagyu flowers are rich in vegetable collagen and are known to be effective in preventing aging, anti-cancer, and adult diseases, and are used in cosmetics, flower tea, and liquor.

CARPINUS TSCHONOSKII is a deciduous tree that grows in the south of Gangwon-do and has a height of 15m and a diameter of 70cm. The bark is gray, small branches have hairs, white bark is scattered, and young leaves and petioles have hairs. The leaves are 2 lines with sharp ends, the bottom of the leaves is round or pointed, 4~8cm long, and has thin and sharp double teeth. There are hairs lying on the veins on the surface and back, and the petiole is 8-12mm long. The flower is unisexual, and the male flower runs one by one in one bract, and there are 4 to 8 stamens. Female flower inflorescence has a stalk, and 2 female flowers run in each bract scale, and each has 1 fat and 2 styles. The flowering period is from April to May, the fruiting period is in October, and the wood is used as raw wood for shiitake. In folk remedies, the sap collected in early spring was called gyeonpunggeon and used for the treatment of osteoporosis, and it was confirmed that the extract of the japonica tree has the effect of protecting cells from harmful substances such as fine dust.

Silkworm (SILKWORM) is a larva of silkworm moth belonging to Lepidoptera Silkworm family. The color of the body is milky white, and it is covered with a soft chitinous shell to give a soft touch. It consists of 13 nodes and has black patterns on its body. When it comes out of the egg, it has black hair, then sheds the hair on the back and turns gray. Every four times they sleep, they take off their eyelids and grow about 8 cm for about 25 days, then vomit the threads to build a cocoon. It pupates in a cocoon and then emerges again as a moth.

Silkworms were raised to produce silk in the past, but are now being produced as raw materials for processing into health supplements. Before silkworms become pupae and make thread bags, they are dried in the sun on the 3rd day of fifth instar (19 days after silkworm shedding), which is the period when they have the most nutrients, made into powder with a grinder, sterilized by quick freezing, and dried to make silkworm powder or silkworm pills. make it Silkworms are known to be highly effective in curing and preventing adult diseases such as diabetes, high blood pressure, liver function improvement, blood sugar lowering, stroke prevention, brain nerve activation, and gastrointestinal diseases.

CENTELLA ASIATICA is a perennial plant belonging to the umbel family Apiaceae. It is distributed in Korea, Japan, China, etc., and is traditionally used in the treatment of various diseases in China and Southeast Asian countries. In Indian and Sri Lankan traditional medicine, it is used to treat skin diseases, syphilis, rheumatism, psychosis, epilepsy, hysteria, dehydration and leprosy, and in Southeast Asian countries, it is used to treat diarrhea, eye diseases, infections, asthma and hypertension.

Centella asiatica mainly contains triterpenoid saponin, triterpene and flavonoid components, protects the gastric mucosa, and has antiviral, anti-inflammatory, antidepressant and antitumor effects. It is used as skin lotion, hair color, anti-dandruff, organic shampoo, hair oil, hair gel, conditioner, skin cream, skin toner, mask pack, cleansing balm, skin moisturizer, and skin serum.

The present invention is an anti-pollution cosmetic composition with excellent anti-oxidation and anti-wrinkle effects, containing camellia extract, geumhwagyu extract, fish extract, silkworm extract and centella asiatica extract as active ingredients.

The prepared camellia flower is pulverized using a grinder. The grinding size is not particularly limited, and may be pulverized to a size of 1 to 3 cm. An extraction solvent of 20 times the weight was added to the pulverized camellia flower and mixed with shaking at 100 rpm for 24 hours at 25 ° C. This is filtered using a filter, and a powdered camellia extract from which the extraction solvent has been removed is prepared using a concentrator.

The extraction solvent is one or more selected from the group consisting of water, alcohol having 1 to 10 carbon atoms, and mixtures thereof.

According to the above method, extracts of sagebrush, sage extract, silkworm extract, and centella asiatica extracts are prepared from sage flowers, leaves, silkworm powder, and centella asiatica.

An anti-pollution cosmetic composition is prepared by combining the camellia extract, geumhwagyu extract, fish extract, silkworm extract, and centella extract in a ratio of 3: 2: 3: 1: 1.

The anti-pollution cosmetic composition containing the camellia extract, geumhwagyu extract, fish extract, silkworm extract and centella asiatica extract as active ingredients can be prepared in any formulation conventionally prepared in the field. The formulation is a solution, suspension, emulsion, paste, gel, cream, lotion, essence, powder, lotion, patch, mask sheet, soap, surfactant-containing cleansing, cleansing cream, cleansing foam, oil, body oil, powder foundation , It may be any one or more selected from the group consisting of emulsion foundation, wax foundation and spray, but is not limited thereto.

<Example 1> Preparation of camellia extract

① Prepare camellia flowers, wash, and pulverize using a grinder.

② 50 g of the pulverized camellia flower was put into 1 L of ethanol. After shaking and mixing this at 25 ° C. for 24 hours at 100 rpm, a filter was used.

③ The filtered solution was prepared using a vacuum rotary concentrator (N-1000S) to prepare a powdered camellia extract from which ethanol was removed.

<Example 2> Preparation of Geumhwagyu extract

① Geumhwagyu flowers were prepared, washed, and then pulverized using a grinder.

② 50 g of the pulverized product of the gold flower was put into 1 L of ethanol. After shaking and mixing this at 25 ° C. for 24 hours at 100 rpm, a filter was used.

③ The filtered solution was prepared using a vacuum rotary concentrator (N-1000S) to prepare a powdered Geumhwagyu extract from which ethanol was removed.

<Example 3> Preparation of resora extract

① The leaves were prepared, washed, and then pulverized using a grinder.

② 50 g of the crushed leaves were put into 1 L of ethanol. After shaking and mixing this at 25 ° C. for 24 hours at 100 rpm, a filter was used.

③ The filtered solution was prepared using a vacuum rotary concentrator (N-1000S) to prepare a powdered extract of ethanol from which ethanol was removed.

<Example 4> Preparation of silkworm extract

① 50g of silkworm powder was put into 1L of ethanol. After shaking and mixing this at 25 ° C. for 24 hours at 100 rpm, a filter was used.

② Powdered silkworm extract from which ethanol was removed was prepared using the filtered solution using a vacuum rotary concentrator (N-1000S).

<Example 5> Centella asiatica extract preparation

① Centella asiatica was prepared, washed, and then pulverized using a grinder.

② 50 g of the centella pulverized material was put into 1 L of ethanol. After shaking and mixing this at 25 ° C. for 24 hours at 100 rpm, a filter was used.

③ Centella asiatica extract in powder form from which ethanol was removed was prepared using the filtered solution using a vacuum rotary concentrator (N-1000S).

<Example 6> Preparation of anti-pollution cosmetic composition

An anti-pollution cosmetic composition was prepared by combining the camellia extract, geumhwagyu extract, fish extract, silkworm extract, and centella asiatica extract prepared in <Example 1> to <Example 6> in an appropriate ratio.

Anti-oxidation and anti-wrinkle tests were conducted on the anti-pollution cosmetic composition of the present invention.

<Test Example 1> Antioxidant test (DPPH assay)

Antioxidation means to suppress or alleviate the progress of oxidation by removing active oxygen. Organisms, including humans, obtain energy such as ATP through a process called respiration. In this process, 2% of the inhaled oxygen is inevitably converted into active oxygen. This active oxygen refers to oxygen with free radicals, and free radicals cause problems such as cell membrane oxidation, acceleration, aging, and DNA modification.

DPPH antioxidant activity measurement is to measure the degree to which DPPH free radicals are reduced by the reaction between DPPH free radicals and antioxidants. DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a stable free radical. It has unshared electrons (N·) in the molecule, but reacts with electrons or free radicals to form a stable structure (N-H). DPPH is purple in aqueous solution, but when free radicals are scavenged, it becomes light yellow with electron donating ability. At this time, the decreasing absorbance value is measured at 515 to 517 nm using a UV / Vis spectrophotometer to measure the free radical scavenging ability.

1-1. sample preparation

① Camellia extract, Geumhwagyu extract, fish extract, silkworm extract, and Centella asiatica extract were stored at 4 ° C, and four types of samples were prepared by combining them in the ratios shown in <Table 1> below.

camellia Geumhwagyu written language silkworm Centella Asiatica combination 1 4 3 2 One - Combination 2 3 2 3 One One combination 3 2 4 2 One One combination 4 2 One One 4 2

② The four types of samples mixed above are serially diluted in 100% ethanol as shown in <Table 2> below.

One 2 3 4 5 6 density(%) 0.8 0.4 0.2 0.1 0.05 0.025

③ Gallic acid is used as a positive control for equivalent value calculation. Gallic acid was dissolved in 100% ethanol at a concentration of 10 mg/mL, and serial dilution was performed in 100% ethanol as shown in Table 3 below. As a negative control, prepare 100% ethanol (expressed as Gallic acid 0 concentration).

One 2 3 4 5 6 (negative control group) Concentration (ug/mL) 240 120 90 60 30 0

④ For samples with color, the absorbance is measured without adding DPPH as a DPPH negative control, and then corrected from the absorbance value of the sample with DPPH added.

1-2. Test Methods

① Add gallic acid and samples to a 96 well plate in three repetitions.

② React at room temperature in a dark room for 30 minutes and invert (a technique of carefully inverting a test tube or tube).

③ Measure the absorbance of the reaction solution at 517 nm using a microplate reader.

1-3. Test result

[Figure 1] is a graph showing the results of the antioxidant test (DPPH assay) of the present invention.

For gallic acid equivalent (GAE), a standard curve was drawn using the absorbance value (y) for each gallic acid concentration (x), and the x value corresponding to the absorbance (y) of the sample was calculated using the equation calculated from the trend line.

Radical scavenging activity was calculated using the following [Equation 1].

EC50 (effective concentration of 50%) draws a curve using the inhibition rate (y) for each concentration (x) of the sample and substitutes 50 for the inhibition rate (y) of the sample using the equation calculated from the trend line to obtain the corresponding x value Calculated.

[Figure 2] is a graph showing the DPPH radical scavenging ability evaluation results of the present invention. As a result of the DPPH test, combination 2 showed the highest antioxidant activity.

An anti-wrinkle test was conducted using the sample of Combination 2 having the highest antioxidant activity.

<Test Example 2> Cytotoxicity evaluation

Dermal fibroblasts are cells that make the fibers that make up the skin. As a cytotoxicity test, there is MTT assay, and the cell viability is confirmed in vitro by processing the sample in CCD-986SK cell, which is a human skin fibroblast.

The MTT assay measures the metabolic activity of cells by color change. The NAD(P)H-dependent oxidoreductase enzyme is present in many cells, and this enzyme is an insoluble form of the dye MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], a tetrazolium dye. reduced with formazan. Originally, MTT reagent is a yellow aqueous solution, but formazan is a blue-purple water-insoluble crystal. Therefore, by dissolving formazan in an organic solvent such as DMSO and measuring the concentration, the cell's dehydrogenase reducing ability can be measured. The activity of dehydrogenase present in the mitochondria of living cells can be measured, and the drug is treated and whether the cells are alive (with dehydrogenase activity) is measured.

In the case of drug treatment, the degree of formation of formazan varies depending on the concentration gradient, and through this, the concentration at which cells are alive at about 50% can be determined, which is called #IC50 value.

2-1. sample preparation

① Samples were prepared using IMDM (Iscove Modified Dulbecco Media) growth media (10% FBS, 1% penicillin/streptomycin) as shown in Table 4 below.

Treatment group name Treatment concentration (%) 1. control - 2. Fine dust compounds 0.2 3. Fine dust compound + combination 2 fine dust 0.2 Combination 2 0.02 4. Combination 2 0.02

② For no-treatment, use IMDM growth media without any treatment.

2-2. Test Methods

① CCD-986SK cells were suspended in IMDM growth media, and 6 × 10 ³ cells were dispensed in three repetitions of 200 μl per well in a 96-well microplate, followed by incubation in a CO ₂ incubator (37℃, 5% CO ₂ ) for 24 hours.

② After culturing for 24 hours, remove the culture medium from the plate.

③ Process 200 μl of sample in each well.

④ After sample processing, incubate for 48 hours in a CO ₂ incubator (37℃, 5% CO ₂ ).

⑤ Remove the treated culture solution/sample.

⑥ MTT medium (50% MTT reagent in serum-free IMDM) is treated with 50 μl of each well and reacted in a CO ₂ incubator for 2 hours.

⑦ Add 150 μl of MTT solvent and shake incubation for 15 minutes.

⑧ Measure the absorbance of the reaction-completed medium at 590 nm reference wavelength using a Microplate reader.

2-3. Test result

[Figure 3] is a graph showing the results of the skin fibroblast cytotoxicity test of the present invention.

Cell viability was calculated using the following [Equation 2].

While cytotoxicity appeared in the fine dust compound treatment group, cytotoxicity tended to recover when co-treated with Combination 2. No cytotoxicity was observed when the combination 2 was treated alone.

Expression levels of wrinkle-related factors were evaluated at the same concentration.

<Test Example 3> Anti-wrinkle test (evaluation of human skin fibroblast anti-wrinkle-related factor expression level)

Samples are processed in CCD-986SK cells, which are skin fibroblasts, to measure the expression of skin elasticity-related enzymes (gelatinase, collagenase, matrix metalloproteinase) and Type 1 collagen (COL1A) genes.

The enzyme of collagenase that degrades collagen or the enzyme that degrades the extracellular matrix in vitro is compared with the blank sample solution to confirm the degree of inhibition of collagenase or the enzyme that degrades the extracellular matrix. Measure the degree of increased synthesis of type 1 collagen, which is greatly reduced in the extracellular matrix of the dermal layer due to

3-1. sample preparation

① Samples are prepared using IMDM growth media (10% FBS, 1% penicillin/streptomycin) as shown in <Table 5> below.

Treatment group name Treatment concentration (%) 1. control - 2. Fine dust compounds 0.2 3. Fine dust compound + combination 2 fine dust 0.2 Combination 2 0.02 4. Combination 2 0.02

② For no-treatment, use IMDM growth media without any treatment.

3-2. Test Methods

① CCD-986SK cells are suspended in IMDM growth media, and 1 × 10 ⁵ cells are dispensed by 2mL per well in a 6-well microplate, and then cultured in a CO ₂ incubator (37℃, 5% CO ₂ ) for 24 hours.

② After culturing for 24 hours, remove the culture medium from the plate.

③ Process 2mL of sample in each well.

⑤ After sample treatment, incubate for 48 hours in a CO ₂ incubator (37℃, 5% CO ₂ ).

⑥ After removing the treatment culture medium, the cells are harvested and RNA is extracted.

⑦ Synthesize cDNA using 4 μg of RNA.

⑧ The primer sequence and PCR conditions of the target gene used for qRT-PCR are shown in <Table 6> and <Table 7> below.

Gene Primer sequence Annealing temp.(℃) GAPDH F-TGCACCACCACCTGCTTAGC
R-GGCATGGACTGTGGTCATGAG 56.9 COL1A F-AGAAACACGTCTGGCTAGGAG
R-GCATGAAGGCAAGTTGGGTAG 56.9 MMP-1 F-AAGGTTAGCTTACTGTCACACGCTT
R-CGACTCTAGAAACACAAGAGCAAGA 60.6

1 cycle 30 cycles 1 cycle 95℃ 95℃ Annealing temp 72℃ 72℃ 3 minutes 30 seconds 30 seconds 1 min 5 minutes

3-3. Test result

Figure 4 is a graph showing the results of analysis of skin fibroblast wrinkle factor expression of the present invention.

For glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which is a control gene (housekeeping gene), the difference (ΔCt) from the threshold cycle Ct value was calculated, and then the △ΔCt value, which is the difference from the △Ct of the untreated group, was calculated. , Finally, the 2 ^-ΔΔCt value was evaluated and analyzed as the expression level of the gene.

There was no change in collagen expression when the control gene was treated with fine dust compounds, but the expression of collagenase was statistically significantly increased. In Combination 2 treatment, the amount of collagen expression increased regardless of whether or not fine dust compound was treated, and the expression of collagenase decreased significantly in Combination 2 treatment.

As described above, the anti-pollution cosmetic composition of the present invention contains natural extracts as an active ingredient, so there is little skin irritation, excellent antioxidant and anti-wrinkle efficacy, and can effectively protect the skin from pollutants such as fine dust. there is.

As described above, an optimal embodiment has been disclosed in the specification. Although specific terms have been used herein, they are only used for the purpose of describing the present invention and are not used to limit the scope of the present invention described in the claims. Therefore, those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. Therefore, the true technical protection scope of the present invention should be determined by the technical spirit of the appended claims.

Claims (11)

An anti-pollution cosmetic composition comprising camellia extract, geumhwagyu extract, fish extract, silkworm extract and centella asiatica extract as active ingredients.
According to claim 1,
The camellia extract is extracted at 100 rpm for 24 hours at 25 ° C. by adding an extraction solvent of 20 times the weight to the pulverized camellia flower, and then filtered and concentrated to remove the extraction solvent. Anti-pollution cosmetic composition, characterized in that the powder state.
According to claim 1,
The gold flower extract is extracted at 100 rpm for 24 hours at 25 ℃ by adding an extraction solvent of 20 times the weight to the ground gold flower, and then filtered and concentrated to remove the extraction solvent Anti-pollution cosmetic composition, characterized in that the powder state .
According to claim 1,
The extract is extracted at 100 rpm for 24 hours at 25 ° C. by adding an extraction solvent of 20 times the weight to the pulverized leaves of the leaves, and then filtered and concentrated to remove the extraction solvent. Anti-pollution cosmetic, characterized in that the powder state composition.
According to claim 1,
The silkworm extract is an anti-pollution cosmetic composition, characterized in that the extracting solvent is added to silkworm powder, extracted at 100 rpm for 24 hours at 25 ° C., filtered and concentrated to remove the extraction solvent.
According to claim 1,
The Centella Asiatica extract is extracted at 100 rpm for 24 hours at 25 ° C. by adding an extraction solvent of 20 times the weight to the pulverized Centella asiatica extract, and then filtered and concentrated to remove the extraction solvent. Anti-pollution cosmetic composition, characterized in that the powder state.
According to claims 2 to 6,
Anti-pollution cosmetic composition, characterized in that the extraction solvent is at least one selected from the group consisting of water, alcohols having 1 to 10 carbon atoms, and mixtures thereof.
According to claim 1,
An anti-pollution cosmetic composition, characterized in that the camellia extract, geumhwagyu extract, fish extract, silkworm extract and centella extract are blended in a ratio of 3: 2: 3: 1: 1.
According to claim 1,
An anti-pollution cosmetic composition characterized by having antioxidant and anti-wrinkle effects.
According to claim 1,
The anti-pollution cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, essence, powder, lotion, patch, mask sheet, soap, surfactant-containing cleansing, cleansing cream, cleansing foam, oil, body An anti-pollution cosmetic composition, characterized in that it is any one or more formulations selected from the group consisting of oil, powder foundation, emulsion foundation, wax foundation and spray.
① pulverizing the prepared camellia flower with a grinder;
② extracting by adding an extraction solvent to the pulverized camellia flower, and filtering through a filter;
③ concentrating the filtered solution in step ② to prepare a powdered camellia extract;
④ Proceeding the above ① to ③ processes with Geumhwagyu flowers to prepare Geumhwagyu extract;
⑤ Proceeding the above steps ① to ③ with resora leaves to prepare an extract of resosa;
⑥ Proceeding the above steps ② to ③ with silkworm powder to produce silkworm extract;
⑦ Proceeding the above steps ① to ③ with centella asiatica to prepare centella asiatica extract; and
⑧ step of blending the camellia extract, geumhwagyu extract, fish extract, silkworm extract and centella asiatica extract in a predetermined ratio;
Method for producing an anti-pollution cosmetic composition comprising a.

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