CN108245469B

CN108245469B - Anti-aging skin care product

Info

Publication number: CN108245469B
Application number: CN201810340299.3A
Authority: CN
Inventors: 刘瑞芳
Original assignee: Shenzhen Zhongdaomei Biotechnology Co ltd
Current assignee: Shenzhen Zhongdaomei Biotechnology Co ltd
Priority date: 2018-04-17
Filing date: 2018-04-17
Publication date: 2021-06-11
Anticipated expiration: 2038-04-17
Also published as: CN108245469A

Abstract

The invention discloses an anti-aging skin care product which comprises, by mass, 20-24% of a wolfberry fruit extract, 16-18% of an ophiopogon root extract, 10-12% of a quince extract, 5-7% of an oat bran extract, 1-2% of guar gum, 4-5% of polyvinylpyrrolidone, 4-5% of polyethylene glycol and 32-35% of base oil, wherein the base oil is selected from rose fruit oil or aloe oil, and has the advantage of anti-aging.

Description

Anti-aging skin care product

Technical Field

The invention relates to an anti-aging skin care product.

Background

Aging refers to the progressive functional and organic degenerative changes that occur over time in an organism. The skin, like other organs of the human body, ages gradually with age, and there are a series of biological changes in the natural aging process of the skin, such as the reduction of cellular components of the skin and subcutaneous tissues and the change of the immune system. The fundamental change of skin aging is the appearance of wrinkles.

Fructus Lycii is mature fruit of Lycium barbarum L. of Solanaceae, and has effects of caring skin, removing speckle, moistening skin, and reducing weight. Mainly contains alkaloids (betaine, etc.), flavonoids, polysaccharides, pigments (carotene, xanthophyll), volatile oils, etc. The wolfberry fruit polysaccharide can eliminate oxygen free radicals, reduce lipofuscin content, delay senility, reduce blood fat, reduce fat in body, reduce fat cell volume and reduce weight, and the beta-carotene and vitamins contained in the wolfberry fruit polysaccharide have anti-aging effect. In the prior art, the medlar is absorbed in an edible way, and the development of other use forms of the medlar has certain production and application values.

Disclosure of Invention

The invention aims to provide an anti-aging skin care product which has the advantage of anti-aging.

The technical purpose of the invention is realized by the following technical scheme:

an anti-aging skin care product comprises, by mass, 20-24% of a wolfberry fruit extract, 16-18% of a dwarf lilyturf root extract, 10-12% of a quince extract, 5-7% of an oat bran extract, 1-2% of guar gum, 4-5% of polyvinylpyrrolidone, 4-5% of polyethylene glycol and 32-35% of base oil; the base oil is selected from rose hip oil or aloe vera oil.

Fructus Lycii is mature fruit of Lycium barbarum L. belonging to Solanaceae, is sweet and neutral, and enters liver and kidney channels, and has effects of nourishing liver and kidney, nourishing liver and improving eyesight. Modern medical research shows that the health care product contains carotene, betaine, multiple vitamins, calcium, phosphorus, iron and the like, has pharmacological effects of increasing cell activity and promoting stem cell regeneration, and also can reduce blood pressure, blood sugar and blood fat.

Radix Ophiopogonis is root tuber of Ophiopogon japonicus (Ophiopogon japonicus, Linn. f.) Ker-Gawl.) belonging to Liliaceae. It has effects in nourishing yin, promoting fluid production, moistening lung, and clearing heart fire, and can be used for treating dry cough due to lung dryness, cough due to consumptive disease, thirst due to body fluid consumption, vexation, insomnia, internal heat, diabetes, constipation due to intestinal dryness, and diphtheria due to pharynx. Modern medicine shows that it contains saponins (ophiopogonin A, B, C, D, etc.), flavones (ophiopogon flavanone A, B, methyl ophiopogon flavanone A, B, etc.), saccharides (monosaccharides and oligosaccharides), volatile oil, beta-sitosterol and stigmasterol.

Quince, quince Cydonia oblonga mill, a quince of the family rosaceae, is administered as a fruit. It has effects of eliminating dampness, relieving summer-heat, relaxing muscles and tendons, and activating collaterals, and can be used for treating sunstroke, emesis, diarrhea, dyspepsia, arthralgia, and gastrocnemius spasm. Modern medicine shows that it contains sugar (mainly fructose), tannin, protopectin, organic acids (malic acid, tartaric acid, citraconic acid) and volatile oil.

Oat bran contains water-soluble dietary fiber, insoluble dietary fiber, linoleic acid, etc.

By adopting the technical scheme, the base oil is used as a carrier, so that the situations of skin burn and the like caused by overhigh concentration of the extract are reduced; the combination of guar gum, polyvinylpyrrolidone and polyethylene glycol is utilized to uniformly disperse the various extracts, so that the phenomena of layering and the like are reduced; utilize mild stimulation of quince extract to the capillary, oat bran extract to the tightening effect of skin and polyvinylpyrrolidone and polyethylene glycol to the softening of skin top layer, realize opening and shutting of skin pore, promote the active ingredient in wolfberry fruit extract, other extracts, guar gum gets into the skin, the ophiopogon root extract can assist the condition that plays the skin allergy of reduction skin opening and shutting in-process simultaneously, consequently, the absorption condition of the product of this application is superior to solitary wolfberry fruit extract far away, have better emollient, moisturizing and anti-aging effect.

More preferably: the beta-carotene in the medlar extract is less than 0.05wt% of the total amount of the anti-aging skin care product.

Modern medical research shows that the beta-carotene contained in the medlar also has the anti-aging effect. However, in this scheme, the reduction of β -carotene can improve the anti-aging effect, which is also the new point of this application. The medlar in the prior art is mainly used in a form of eating, and researches show that the beta-carotene in the medlar can improve the anti-aging effect; however, in the present application, acting directly on the skin in the form of a skin care product, it is speculated that the skin has a hindering effect on its absorption, causing a phenomenon which is contrary to the teaching in the prior art.

More preferably: the wolfberry fruit extract is prepared by the following method:

(1) taking fresh fructus Lycii, air drying until the water content of fructus Lycii is not higher than 10 wt%;

(2) taking the dried medlar, adding water, and mechanically crushing to obtain medlar water slurry; the ratio of the dry weight of the medlar to the amount of the water added in the step (2) is 1: 2.0-2.2 by mass;

(3) transferring the wolfberry fruit water slurry into a reflux device, adding water, heating to 88-92 ℃, carrying out heat preservation reaction for 3.0-3.2 hours, and naturally cooling to below 30 ℃; filtering with water system microporous membrane to obtain fructus Lycii filtrate; the ratio of the dry weight of the medlar to the amount of the water added in the step (3) is 1: 1.2-1.5 by mass;

(4) heating the wolfberry fruit filtrate to 45-50 ℃, dropwise adding diethyl ether while stirring, uniformly mixing, cooling to a temperature lower than 30 ℃, standing for layering, and taking a water layer; repeating for 2-3 times; the dosage ratio of the wolfberry fruit filtrate to the ether added each time is 1: 0.05-0.10 by mass;

(5) taking the water layer, freeze-drying at-30 to-35 ℃ and under 20 to 25Pa, and concentrating to 50 to 55wt% of the initial water layer to obtain the wolfberry fruit extract.

By adopting the technical scheme, the content of beta-carotene in the obtained wolfberry extract is low, and the yield is ensured.

More preferably: in the step (3) of preparing the wolfberry fruit extract, adding microcrystalline cellulose and polyvinylpyrrolidone while adding water, heating to 75-78 ℃, and reacting for 1.3-1.5 hr under heat preservation; the weight ratio of the dry weight of the medlar and the amount of the water, the microcrystalline cellulose and the polyvinylpyrrolidone in the step (3) is 1: 1.2-1.5: 0.022-0.025: 0.030-0.033.

By adopting the technical scheme, the reaction temperature is reduced, the heat preservation time is shortened, the possibility of increasing byproducts is reduced, and the yield and/or the purity are/is improved.

More preferably: in the step (4) of preparing the medlar extract, diethyl ether is added for the first time, and sodium chloride and sodium bicarbonate are added at the same time; the dosage ratio of the ethyl ether, the sodium chloride and the sodium bicarbonate added into the medlar filtrate each time is 1: 0.05-0.10: 0.002-0.003 by mass.

By adopting the technical scheme, the micro-emulsification phenomenon during standing separation is reduced, the separation difficulty is reduced, and the yield and/or purity are/is improved.

More preferably: the radix ophiopogonis extract is prepared by the following method:

taking dried tuber roots of radix ophiopogonis, crushing, and sieving with a 50-100-mesh sieve; putting the dried root tuber powder of the dwarf lilyturf tuber into a reflux device, adding water, heating to 88-90 ℃, and reacting for 40-45 min under the condition of heat preservation; cooling to 60-62 ℃ in an ice water bath, adding ethanol, reacting for 25-30 min under heat preservation, and filtering with an organic microporous filter membrane to obtain a radix ophiopogonis extracting solution; taking the radix ophiopogonis extracting solution, freeze-drying at-10 to-15 ℃ and 10-15 Pa, and concentrating to 33-35 wt% of the amount of the initial radix ophiopogonis extracting solution to obtain the radix ophiopogonis extract;

the usage ratio of the dried root tuber of the dwarf lilyturf tuber, water and ethanol is 1: 4.5-4.8: 1.2-1.3 by mass.

More preferably: the oat bran extract is prepared by the following method:

taking dry oat bran, putting the dry oat bran into 85-97% RH, absorbing water for 12-15 hr, and extruding at 120-125 ℃ to obtain pasty oat bran; putting the pasty oat bran into a reflux device, adding water and ethanol, heating to 75-77 ℃, carrying out reflux reaction for 2.5-2.8 hr, and filtering with an organic microporous filter membrane to obtain an oat bran extract; freeze-drying oat bran extract at-10 to-15 ℃ and 10-15 Pa, and concentrating to 40-45 wt% of the amount of the initial oat bran extract to obtain an oat bran extract;

the usage ratio of the dry oat bran, water and ethanol is 1: 2.0-2.2: 3.6-3.8 by mass.

More preferably: the quince extract is prepared by the following method:

taking quince dried fruits, crushing, and sieving with a 50-100-mesh sieve; putting quince dried fruit powder into a reflux device, adding water, polyamide and polyvinylpyrrolidone, heating to 88-90 ℃, and reacting for 90-100 min in a heat preservation manner; cooling to 68-70 ℃ in an ice water bath, adding ethanol, reacting for 20-22 min under heat preservation, and filtering with an organic microporous filter membrane to obtain a quince extracting solution; taking quince extract, freeze-drying at-10 to-15 ℃ and 10-15 Pa, and concentrating until the content of the quince extract is 33-40 wt% of the initial amount to obtain quince extract;

according to the mass ratio of the quince dried fruits, the water, the polyamide, the polyvinylpyrrolidone and the ethanol is 1: 2.5-2.8: 0.020-0.022: 0.028-0.029: 3.0-3.2.

In conclusion, the invention has the following beneficial effects:

the capacity of removing free radicals is strong, and the oxidation resistance is strong; the skin wrinkle is improved obviously, and the anti-aging effect is better.

Detailed Description

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the present invention.

Example 1 a: a fructus Lycii extract is prepared by the following method:

(1) taking fresh medlar, and airing until the water content of the medlar is 5 wt%;

(2) taking the dried medlar, adding water, and mechanically crushing to obtain medlar water slurry; the dry weight of the medlar and the dosage of the water added in the step (2) are 1:2.0 by mass;

(3) transferring the fructus Lycii water slurry to a reflux device, adding water, microcrystalline cellulose and polyvinylpyrrolidone, heating to 75 deg.C, reacting for 1.5hr, and naturally cooling to 20 deg.C; filtering with 0.22 μm water system microporous membrane to obtain fructus Lycii filtrate; the use amount ratio of the dry weight of the medlar, the water added in the step (3), the microcrystalline cellulose and the polyvinylpyrrolidone is 1:1.5:0.022:0.030 by mass;

(4) heating fructus Lycii filtrate to 45 deg.C, adding diethyl ether while stirring, mixing, cooling to 25 deg.C, standing for layering, and collecting water layer; repeating for 2 times, adding diethyl ether for the first time, and adding sodium chloride and sodium bicarbonate; according to the mass ratio of the wolfberry fruit filtrate to the added diethyl ether, sodium chloride and sodium bicarbonate every time is 1:0.05:0.003: 0.003;

(5) freeze-drying the water layer at-30 deg.C and 25Pa, and concentrating to 50wt% of the initial water layer to obtain fructus Lycii extract.

Example 1 b: a fructus Lycii extract is prepared by the following method:

(1) taking fresh medlar, and airing until the water content of the medlar is 8 wt%;

(2) taking the dried medlar, adding water, and mechanically crushing to obtain medlar water slurry; according to the mass, the dry weight of the medlar and the dosage of the water added in the step (2) are 1: 2.2;

(3) transferring the fructus Lycii water slurry to a reflux device, adding water, microcrystalline cellulose and polyvinylpyrrolidone, heating to 78 deg.C, reacting for 1.3hr, and naturally cooling to below 28 deg.C; filtering with 0.45 μm water system microporous membrane to obtain fructus Lycii filtrate; the dry weight of the medlar and the dosage ratio of the water, the microcrystalline cellulose and the polyvinylpyrrolidone in the step (3) are 1:1.2:0.025:0.033 by mass;

(4) heating fructus Lycii filtrate to 50 deg.C, adding diethyl ether while stirring, mixing, cooling to 28 deg.C, standing for layering, and collecting water layer; repeating the steps for 3 times, and adding sodium chloride and sodium bicarbonate while adding diethyl ether for the first time; according to the mass ratio of the added ether, sodium chloride and sodium bicarbonate to the wolfberry fruit filtrate every time is 1:0.10:0.002: 0.002;

(5) freeze drying the water layer at-35 deg.C under 20Pa, and concentrating to 55wt% of the original water layer to obtain fructus Lycii extract.

Example 1 c: a kind of Lycium barbarum fruit extract is different from that in example 1a in that in step (4), diethyl ether is added for the first time and sodium chloride or sodium bicarbonate is not added. Compared with the example 1a, the microemulsion phenomenon appears when the step (4) is kept stand for separation, and the microemulsion part is difficult to separate, thereby influencing the yield and/or the purity.

Example 1 d: a kind of fructus Lycii extract, different from example 1a, in step (3), add water and not add microcrystalline cellulose or polyvinylpyrrolidone, heat up to 88 deg.C, keep warm and react for 3.2 hr. Compared to example 1a, the reaction temperature is increased, the incubation time is prolonged, and there is a possibility that by-products are increased, affecting the yield and/or purity.

Example 1 e: a kind of fructus Lycii extract, different from example 1b, in step (3), add water and not add microcrystalline cellulose or polyvinylpyrrolidone, heat up to 92 deg.C, keep warm and react for 3.0 hr. Compared to example 1b, the reaction temperature is increased, the incubation time is prolonged, and there is a possibility that by-products are increased, affecting the yield and/or purity.

Example 1 f: a fructus Lycii extract is prepared from 997.2g of the fructus Lycii extract of example 1a and 2.8g of beta-carotene by mixing.

Example 2 a: an ophiopogon root extract is prepared by the following method:

taking dried tuber of dwarf lilyturf tuber, crushing and sieving by a 100-mesh sieve; placing radix Ophiopogonis dried root tuber powder into a reflux device, adding water, heating to 88 deg.C, and reacting for 45min under heat preservation; cooling to 60 deg.C in ice water bath, adding ethanol, reacting for 30min under heat preservation, and filtering with 0.22 μm organic microporous membrane to obtain radix Ophiopogonis extract; freeze-drying radix Ophiopogonis extract at-15 deg.C under 15Pa, and concentrating to 35wt% of the original radix Ophiopogonis extract to obtain radix Ophiopogonis extract; the dosage ratio of the dried root tuber of the dwarf lilyturf tuber, water and ethanol is 1:4.5:1.2 by mass.

Example 2 b: an ophiopogon root extract is prepared by the following method:

taking dried tuber of dwarf lilyturf tuber, crushing and sieving by a 50-mesh sieve; placing radix Ophiopogonis dried root tuber powder into a reflux device, adding water, heating to 90 deg.C, and reacting for 40 min; cooling to 62 deg.C in ice water bath, adding ethanol, reacting for 25min under heat preservation, and filtering with 0.45 μm organic microporous membrane to obtain radix Ophiopogonis extract; freeze-drying radix Ophiopogonis extract at-10 deg.C under 10Pa, and concentrating to 33wt% of the original radix Ophiopogonis extract to obtain radix Ophiopogonis extract; the dosage ratio of the dried root tuber of the dwarf lilyturf tuber, water and ethanol is 1: 4.8: 1.3 by mass.

Example 3 a: an oat bran extract prepared by the following method:

taking dried oat bran, placing into 85% RH, absorbing water for 15hr, and squeezing at 120 deg.C to obtain paste oat bran; placing the paste oat bran into a reflux device, adding water and ethanol, heating to 75 deg.C, reflux reacting for 2.8hr, and filtering with 0.22 μm organic microporous membrane to obtain oat bran extract; freeze drying oat bran extract at-15 deg.C under 15Pa, and concentrating to 40wt% of original oat bran extract to obtain oat bran extract; the dosage ratio of the dry oat bran, the water and the ethanol is 1:2.0:3.6 by mass.

Example 3 b: an oat bran extract prepared by the following method:

taking dried oat bran, placing into 97% RH, absorbing water for 12hr, and squeezing at 125 deg.C to obtain paste oat bran; placing the paste oat bran into a reflux device, adding water and ethanol, heating to 77 deg.C, reflux reacting for 2.5hr, and filtering with 0.45 μm organic microporous membrane to obtain oat bran extract; freeze drying oat bran extract at-10 deg.C under 10Pa, and concentrating to 45wt% of original oat bran extract to obtain oat bran extract; the dosage ratio of the dry oat bran, the water and the ethanol is 1:2.2: 3.8 by mass.

Example 4 a: a quince extract prepared by the following method:

taking quince dried fruits, crushing, and sieving with a 100-mesh sieve; putting quince dried fruit powder into a reflux device, adding water, polyamide and polyvinylpyrrolidone, heating to 88 deg.C, and reacting for 100 min; cooling to 68 deg.C in ice water bath, adding ethanol, reacting for 22min under heat preservation, and filtering with 0.22 μm organic microporous filter membrane to obtain Cydonia oblonga extractive solution; freeze drying the quince extract at-10 deg.C and 10Pa, and concentrating to 33wt% of the original amount of quince extract to obtain quince extract; the amount ratio of the quince dried fruit, water, polyamide, polyvinylpyrrolidone and ethanol was 1:2.5:0.020:0.028:3.0 by mass.

Example 4 b: a quince extract prepared by the following method:

taking quince dried fruits, crushing, and sieving with a 50-mesh sieve; putting quince dried fruit powder into a reflux device, adding water, polyamide and polyvinylpyrrolidone, heating to 90 ℃, and reacting for 90min under heat preservation; cooling to 70 deg.C in ice water bath, adding ethanol, reacting for 20min under heat preservation, and filtering with 0.45 μm organic microporous filter membrane to obtain fructus Cydoniae Oblongae extractive solution; freeze drying the quince extract at-15 deg.C and 15Pa, and concentrating to 40wt% of the original amount of quince extract to obtain quince extract; the amount ratio of the quince dried fruit, water, polyamide, polyvinylpyrrolidone and ethanol was 1:2.8:0.022:0.029:3.2 by mass.

Example 5: an anti-aging skin care product is prepared by uniformly mixing the following components by mass:

wolfberry extract (from example 1 a) 20%, ophiopogon root extract (from example 2 a) 18%, quince extract (from example 4 a) 12%, oat bran extract (from example 3 a) 5%, guar 1%, polyvinylpyrrolidone 5%, polyethylene glycol 4% and base oil (rose oil, commercially available from guangzhou yao cosmetics limited) 35%.

Example 6: an anti-aging skin care product is prepared by uniformly mixing the following components by mass:

wolfberry extract (from example 1 b) 24%, ophiopogon root extract (from example 2 b) 16%, quince extract (from example 4 b) 10%, oat bran extract (from example 3 b) 7%, guar gum 2%, polyvinylpyrrolidone 4%, polyethylene glycol 5% and base oil (aloe vera, commercially available from Jiangxi Thailand Natural perfumes, Inc.) 32%.

Example 7: an anti-aging skin care product, which is different from example 5 in that the extract of Lycium barbarum is derived from example 1 b.

Example 8: an anti-aging skin care product, which differs from example 5 in that the extract of Lycium barbarum is derived from example 1 f.

Example 9: an anti-aging skin care product, which is different from example 5 in that the extract of Lycium barbarum is commercially available from Ci-Anthuri Biotech Co.

Example 10: an anti-aging skin care product, which is different from the anti-aging skin care product in example 5, wherein the ophiopogon root extract is commercially available from Shanxi rattan biotech GmbH.

Example 11: an anti-aging skin care product, which is different from example 5 in that quince extract is commercially available from biotechnological limited fruits in leyu, guangzhou.

Example 12: an anti-aging skin care product, which is different from the skin care product obtained in example 5, wherein the oat bran extract is commercially available from Shaanxi Fuwa Natural products Co.

Concentration test of Lycium barbarum pigment (referenced as beta-carotene) in Lycium barbarum extract

(1) Selection of wavelength

Precisely weighing 5.1mg of beta-carotene, dissolving with ethanol, fixing the volume to 100ml, and shaking up to prepare a standard solution; taking 1ml of standard solution, using ethanol to fix the volume to 10ml, shaking up, using ethanol as a blank control, scanning within the wavelength range of 200-600 nm, and determining the maximum absorption peak.

(2) Drawing of standard curve

Respectively transferring 0.1 ml, 0.2 ml, 0.3 ml, 0.4 ml and 0.5ml of the standard solution into a 10ml volumetric flask, metering the volume with ethanol, shaking up, and measuring the absorbance under the maximum absorption peak; taking the concentration of beta-carotene as abscissa and the absorbance under the maximum absorption peak as ordinate, making standard curve and fitting linearly to obtain regression equation, R of the standard curve²Is 0.9994.

(3) Sample testing

The solvent of the test object (examples 1a to 1f, commercially available) was used as a blank control, and the absorbance of the test object at the maximum absorption peak was measured to determine the concentration of β -carotene according to the regression equation.

The test results are shown in table 1. Table 1 shows that the beta-carotene is much reduced, essentially negligible, in examples 1a-1e compared to commercially available wolfberry extracts and example 1f with the addition of beta-carotene to example 1 a.

In vitro antioxidant capacity determination

The determination principle is as follows: diphenylbitter acyl radical (DPPH, C)₁₈H₁₂N₅O₆) Is a stable free radical with nitrogen as the center, and if the tested substance can remove it, it indicates that the tested substance has the function of removing hydroxyl free radical, alkyl free radical or peroxy free radical, etc. The aqueous ethanol DPPH solution is dark blue and strongly absorbs at 520nm, and the absorbance of the solution can be measured by a spectrophotometer, and after the sample is added, the absorbance is reduced due to the removal of DPPH. The ability of the test substance to scavenge DPPH can be evaluated based on the change in absorbance.

And (3) sample determination: 4ml of a pH value 6.88 standard phosphate buffer solution and 4ml of 0.1777mol/l DPPH solution are sequentially added into a 10ml test tube and are uniformly mixed; secondly, adding the sample solution to be detected, supplementing the volume to 10.00ml with distilled water, and fully and uniformly mixing; ③ measuring the absorbance at 520nm after 10 min; each group of samples was measured three times repeatedly and the average value was taken; (iv) clearance of DPPH free radical = [1- (a1-a2)/A3] × 100%; a1 represents the absorbance of DPPH solution after the reaction of adding a sample to be detected; a2 represents the absorbance of a solution containing a sample to be tested and water without DPPH; a3 represents the absorbance of a solution of DPPH and water alone, without the test sample.

The results are shown in Table 2. Table 2 shows that (1) examples 5-12 have a higher DPPH radical scavenging rate than commercially available wolfberry extracts and example 1a, preferably in combination with the components of the present application; ② example 5 has higher DPPH free radical scavenging rate compared to example 8 and example 9, which shows that under the formulation of the present application, the beta-carotene in the medlar extract is preferably less than 0.05wt% (0.056 wt% in example 8, 0.1902wt% in example 9) of the total amount of an anti-aging skin care product; ③ example 5 has a higher DPPH radical scavenging rate than examples 10-12, indicating that the ophiopogon root extract, quince extract and oat bran extract prepared according to the present invention are preferably used in the formulation according to the present application.

Measurement of wrinkles on human skin

The important characteristic of skin aging in the formation of wrinkles, and the measurement of skin wrinkles, is important for judging the degree of skin aging. The present application employs profilometry (indirect measurement).

The test principle is as follows: firstly, a silicone film is made of silicone liquid, and an inverse copy of a skin wrinkle with a specific shape on the skin of a subject is obtained, wherein the part with the wrinkle on the film is convex, and the part without the wrinkle is concave. When a beam of light with specific wavelength irradiates the film, the light transmittance of the convex part is small, the light transmittance of the concave part is large, and the degree of wrinkles is judged and quantified according to the light transmittance. During measurement, light signals of different parts of the membrane are collected by a CCD camera lens, a three-dimensional image of the skin can be obtained through photoelectric and digital processing, and then the parameters of different skin wrinkles can be obtained through analysis by special software.

The testing steps are as follows: firstly, selecting effective volunteers with the age of 25-35 years (except for pregnant or lactating women), wherein the test environment temperature is 20-22 ℃, and the test environment humidity is 40% -60%; selecting a skin wrinkle tester SV600 produced by German CK company and correcting the tester; thirdly, before the experiment, a test subject uses a uniform mild detergent to clean a test part (face), a square experiment area with the size of 3cm multiplied by 3cm and symmetrical position is marked on the tested part, the experiment area is numbered, and a test area and a blank contrast are randomly marked; fourthly, coating siloxane liquid on the skin to prepare a siloxane membrane to obtain an inverse copy of the skin wrinkles of the test area and the blank control area on the skin of the detected person; irradiating the membrane with light with certain wavelength by using an image analysis tester, collecting optical signals of different parts of the membrane by using a CCD camera lens, performing photoelectric and digital processing to obtain a three-dimensional image of the skin, and analyzing by using self-contained software to obtain the average roughness (Rz) and the maximum roughness (Rm). Smearing samples in the test area respectively, and testing the data of using the samples for 0 day, 30 days and 180 days, wherein each smearing is 2ml, and each smearing is performed once a day; each sample corresponds to 10 subjects and the results are averaged.

The results are shown in Table 3. Table 3 shows that (i) both the average roughness (Rz) and the maximum roughness (Rm) were increased using example 1a, and (ii) both the average roughness (Rz) and the maximum roughness (Rm) were decreased using examples 5-12, indicating that the present application can function to reduce wrinkles; ② example 5 has a greater reduction in average roughness (Rz) and maximum roughness (Rm) than example 8 and example 9, indicating that under the formulations of the present application, preferably β -carotene in wolfberry extract is less than 0.05wt% of the total amount of an anti-aging skin care product (0.056 wt% in example 8, 0.1902wt% in example 9); ③ example 5 had a greater reduction in average roughness (Rz) and maximum roughness (Rm) than in examples 10-12, indicating that the ophiopogon root extract, quince extract and oat bran extract prepared herein are preferred for use in the formulations herein.

Claims

1. An anti-aging skin care product is characterized by comprising 20-24% of a wolfberry fruit extract, 16-18% of an ophiopogon root extract, 10-12% of a quince extract, 5-7% of an oat bran extract, 1-2% of guar gum, 4-5% of polyvinylpyrrolidone, 4-5% of polyethylene glycol and 32-35% of base oil by mass; the base oil is selected from rose hip oil or aloe oil;

the beta-carotene in the medlar extract is less than 0.05wt percent of the total weight of the anti-aging skin care product; the wolfberry fruit extract is prepared by the following method:

(1) taking fresh fructus Lycii, air drying until the water content of fructus Lycii is not higher than 10 wt%; (2) taking the dried medlar, adding water, and mechanically crushing to obtain medlar water slurry; the ratio of the dry weight of the medlar to the amount of the water added in the step (2) is 1: 2.0-2.2 by mass; (3) transferring the wolfberry fruit water slurry into a reflux device, adding water, heating to 88-92 ℃, carrying out heat preservation reaction for 3.0-3.2 hours, and naturally cooling to below 30 ℃; filtering with water system microporous membrane to obtain fructus Lycii filtrate; the ratio of the dry weight of the medlar to the amount of the water added in the step (3) is 1: 1.2-1.5 by mass; (4) heating the wolfberry fruit filtrate to 45-50 ℃, dropwise adding diethyl ether while stirring, uniformly mixing, cooling to a temperature lower than 30 ℃, standing for layering, and taking a water layer; repeating for 2-3 times; the dosage ratio of the wolfberry fruit filtrate to the ether added each time is 1: 0.05-0.10 by mass; (5) taking a water layer, freeze-drying at-30 to-35 ℃ and under 20-25 Pa, and concentrating to 50-55 wt% of the initial water layer to obtain a wolfberry fruit extract;

the radix ophiopogonis extract is prepared by the following method: taking dried tuber roots of radix ophiopogonis, crushing, and sieving with a 50-100-mesh sieve; putting the dried root tuber powder of the dwarf lilyturf tuber into a reflux device, adding water, heating to 88-90 ℃, and reacting for 40-45 min under the condition of heat preservation; cooling to 60-62 ℃ in an ice water bath, adding ethanol, reacting for 25-30 min under heat preservation, and filtering with an organic microporous filter membrane to obtain a radix ophiopogonis extracting solution; taking the radix ophiopogonis extracting solution, freeze-drying at-10 to-15 ℃ and 10-15 Pa, and concentrating to 33-35 wt% of the amount of the initial radix ophiopogonis extracting solution to obtain the radix ophiopogonis extract; the using amount ratio of the dried root tuber of the dwarf lilyturf to water to ethanol is 1: 4.5-4.8: 1.2-1.3 by mass;

the oat bran extract is prepared by the following method: taking dry oat bran, putting the dry oat bran into 85-97% RH, absorbing water for 12-15 hr, and extruding at 120-125 ℃ to obtain pasty oat bran; putting the pasty oat bran into a reflux device, adding water and ethanol, heating to 75-77 ℃, carrying out reflux reaction for 2.5-2.8 hr, and filtering with an organic microporous filter membrane to obtain an oat bran extract; freeze-drying oat bran extract at-10 to-15 ℃ and 10-15 Pa, and concentrating to 40-45 wt% of the amount of the initial oat bran extract to obtain an oat bran extract; according to the mass ratio of the dry oat bran to the water to the ethanol is 1: 2.0-2.2: 3.6-3.8;

the quince extract is prepared by the following method: taking quince dried fruits, crushing, and sieving with a 50-100-mesh sieve; putting quince dried fruit powder into a reflux device, adding water, polyamide and polyvinylpyrrolidone, heating to 88-90 ℃, and reacting for 90-100 min in a heat preservation manner; cooling to 68-70 ℃ in an ice water bath, adding ethanol, reacting for 20-22 min under heat preservation, and filtering with an organic microporous filter membrane to obtain a quince extracting solution; taking quince extract, freeze-drying at-10 to-15 ℃ and 10-15 Pa, and concentrating until the content of the quince extract is 33-40 wt% of the initial amount to obtain quince extract; according to the mass ratio of the quince dried fruits, the water, the polyamide, the polyvinylpyrrolidone and the ethanol is 1: 2.5-2.8: 0.020-0.022: 0.028-0.029: 3.0-3.2.

2. The anti-aging skin care product according to claim 1, wherein in the step (3) of preparing the wolfberry fruit extract, water is added, microcrystalline cellulose and polyvinylpyrrolidone are added, the temperature is raised to 75-78 ℃, and the reaction is carried out for 1.3-1.5 hr; the weight ratio of the dry weight of the medlar and the amount of the water, the microcrystalline cellulose and the polyvinylpyrrolidone in the step (3) is 1: 1.2-1.5: 0.022-0.025: 0.030-0.033.

3. The anti-aging skin care product according to claim 1 or 2, wherein in the step (4) of preparing the wolfberry fruit extract, the diethyl ether is added for the first time and the sodium chloride and the sodium bicarbonate are added at the same time; the dosage ratio of the ethyl ether, the sodium chloride and the sodium bicarbonate added into the medlar filtrate each time is 1: 0.05-0.10: 0.002-0.003 by mass.